JP2827649B2 - Channel filters used in liquid chromatographs for blood components - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a flow path filter applicable to a liquid chromatograph for blood components.

[0002]

2. Description of the Related Art When trace components in blood are directly analyzed by high performance liquid chromatography, blood cell membranes, fibers,
Lipid kilomicrons, solid suspended matter, and the like are adsorbed to the separation column, which significantly shortens the life of the column. Since the separation column for high performance liquid chromatography is expensive, the analysis cost per sample increases. To prevent such column contamination, Graham Ellis et al., (Clinical Chemistry, Vol. 30, p. 1746-1752).
As shown in the article of 1984, an off-line centrifugation operation and a filtration operation using a disposable syringe-type filter are generally used.

[0003]

The operation of the above-mentioned known example is complicated, and is not suitable for a case where a quick measurement is required. There is also a method in which a guard column is placed in front of the separation column, but the use of expensive packing materials increases the cost. Metal screens and sintered filters are sometimes used in high-performance liquid chromatography as flow path filters, but these are coarse and contain fine particles, such as hemolyzed blood samples, and are complex and diverse. In many cases, such substances pass through, and do not fulfill a sufficient filtering function. Ma
Also, the flow spreads vertically when passing through the flow path filter.
When it falls, it diffuses in the flow direction. Thus, the expansion in the flow direction
If it is scattered, the analysis accuracy will decrease.

An object of the present invention is to provide an efficient flow path filter.
An object of the present invention is to make it possible to use blood chromatographs to analyze trace components in blood with high accuracy and high accuracy .

[0005]

[MEANS FOR SOLVING THE PROBLEMS] To achieve the above object
In the present invention, the housing having a flat surface,
A first passage provided through the interior of the housing;
A cartridge having a flat surface;
A second passage penetrating therethrough, and the housing
The flat surface of the cartridge and the flat surface of the cartridge.
Having a glass fiber filter paper sandwiched and enclosed,
The eluent guided to the opening of the passage 1 is the glass fiber filter paper.
Through the opening of the second passage, and
The first opening and the second opening are mutually separated by the glass fiber filter paper.
The housing and the cart so that
The ridge was arranged.

[0006]

[Effect] Since it is configured as described above, first,
Use liquid fiber filter paper and apply liquid chromatography in the paper surface direction.
Since the mobile phase of the graph flows, the fine particles in the blood
Contaminants can be removed efficiently. Furthermore, the eluent is on paper
Flow direction, so it is restricted by the thickness of the fiber filter paper,
Since the diffusion in the direction is suppressed, the analysis accuracy is improved.

As described above, the flow path in the cartridge is
If it is displaced from the flow path, the
The sample will flow through the filter for a longer time.
As a result, filtration efficiency and filter life can be significantly improved.

Preferably, an organic silane is added to the glass fiber filter paper.
Upon contact with the compound, the silanol group is modified and
The surface charge changes. This process is suitable for the substance to be measured
Pollutants can be removed. For example, aminosi
When using a run compound, the surface of the treated glass fiber filter paper
Is positively charged at a pH near neutrality. Then it becomes negatively charged
Molecules are more likely to be adsorbed. Therefore charged contaminants
It is more effective in removing quality.

[0009]

Embodiments of the present invention will be described below with reference to the drawings.

First, as shown in FIG. 1, the basic structure of the filter is such that a cartridge body 4 in which porous filters 6a and 6b are sealed with a filter cap 5 is housed in a housing, and is connected to a flow path pipe. To use. FIG. 1A shows a configuration diagram when the cartridge is housed in a housing. FIG. 1B shows a configuration diagram in a state where the housing is disassembled and the cartridge is exposed. The cartridge retainer 2 is separated from the cover housing 1 so that the piping can be prevented from being twisted. FIG.
Indicates the configuration of the cartridge portion. Next, the features of the present invention
The part is shown in FIG. FIG. 3 is a schematic longitudinal sectional view of a cartridge in which the flow path of the cartridge is different from the flow path of the piping system. A glass fiber filter paper (made by Advantech Toyo) cut out in a circular shape is sealed as porous filters 6a and 6b. This cartridge was mounted in a housing connected to a flow channel tube and used. In FIG. 3, since the flow path of the cartridge is different from the flow path of the piping system, the liquid containing the sample flows through the filter. Therefore, the surface area of the passing filter was 10 times or more, and the filtration efficiency was increased. Figure 4 is a schematic diagram of a configuration of a high performance liquid chromatograph using a channel filter. The flow path filter 13 is placed between the injection port 12 and the separation column 14 to prevent the separation column 14 from deteriorating. Hereinafter, an example of a glycohemoglobin analyzer in whole blood will be described.

[0011]

[Table 1]

Table 1 shows the relative values of the hemolyzed filtrate obtained using the filter of FIG. 1 (in each case using one and two porous filters) and the hemolyzed filtrate obtained using the filter of FIG. The experimental result which compared the scattered light intensity with the hemolytic filtrate relative scattered light intensity when the conventional metal channel filter was used and when the filter was not used is shown . It can be seen that the effect is particularly improved when the filter shown in FIG. 3 and the two porous filters 6 shown in FIG. 1 are used. Further, the glass fiber filter paper is immersed in an aqueous solution of an organic silane compound (for example, 3- (2-aminoethylaminopropyl) trimethoxysilane) for 1 hour, heated at about 100 ° C. for 2 hours, cut out, and used as a filter. As a result, in the glycated hemoglobin analyzer in whole blood, the pressure rise quality of the separation column was reduced by about 30% as compared with the untreated filter. Although such aminosilation was effective in the measurement of glycated hemoglobin,
It is considered that another silane compound is effective in measuring other components. FIG. 5 shows a chromatogram of glycated hemoglobin in blood. When a conventional metal filter is used (FIG. 5)
(A)) and a case using a glass fiber filter (FIG. 5)
A similar chromatogram is obtained in (b)) .

[0013]

According to the present invention, as described above,
Can efficiently remove particulate contaminants contained in blood
In addition, the analysis accuracy can be improved by suppressing the diffusion in the flow direction.
You.

[Brief description of the drawings]

[1] channel basic configuration diagram of a filter embodiment.

FIG. 2 is a configuration diagram of a filter cartridge.

FIG. 3 is a longitudinal sectional view of an embodiment of a filter having a step in a flow channel according to the present invention.

FIG. 4 is a configuration diagram of a high-performance liquid chromatograph for blood components.

FIG. 5 shows the results of blood glycohemoglobin analysis.

[Explanation of symbols]

1. Cover housing 2. Cartridge holder 3.
... sealing sheet, 4 ... cartridge body,
5a, 5b: cartridge cap, 6a, 6b: porous filter, 7: storage housing, 8: tube connector, 9: piping tube, 10: switching valve, 11
... pump, 12 ... injection port, 13 ... flow path filter, 1
4: separation column, 15: detector, 16: display,
17 ... Eluent

──────────────────────────────────────────────────続 き Continuing from the front page (72) Inventor Yoshinori Takada 882 Ma, Katsuta-shi, Ibaraki Hitachi, Ltd.Measurement Instruments Division (72) Inventor Mitsuo Ito 882 Ma, Katsuta-shi, Katsuta-shi, Ibaraki Hitachi, Ltd. Measuring Instruments Division (56) References JP-A-2-22654 (JP, A) JP-A-56-150353 (JP, A) JP-A 1-162661 (JP, U) JP-A 59-108269 (JP) , U) Jikai 58-19716 (JP, U) (58) Field surveyed (Int. Cl. ⁶ , DB name) G01N 30/26

Claims (2)

(57) [Claims] A housing having a flat surface;
A first passage extending through the interior of the jing;
Cartridge having a flexible surface and the inside of the cartridge
A second passage provided through the housing;
Hold the flat surface of the cartridge in contact with the flat surface of the cartridge.
Having a glass fiber filter paper embedded and enclosed therein, wherein the first
The eluent guided to the opening of the passage passes through the glass fiber filter paper.
And guided to the opening of the second passage, and the first
An opening and the second opening are mutually separated by the glass fiber filter paper;
The housing and the cartridge so as to be shifted in the plane direction.
Liquid chromatograph for blood components characterized by disposing
Channel filter used for graph. 2. The glass fiber filter according to claim 1, wherein
Blood formation characterized by contacting with an organosilane compound
A flow path filter used for shared liquid chromatography.