JP2754201B2 - Feeding stimulants for the scarab stink bug - Google Patents
Feeding stimulants for the scarab stink bugInfo
- Publication number
- JP2754201B2 JP2754201B2 JP8166642A JP16664296A JP2754201B2 JP 2754201 B2 JP2754201 B2 JP 2754201B2 JP 8166642 A JP8166642 A JP 8166642A JP 16664296 A JP16664296 A JP 16664296A JP 2754201 B2 JP2754201 B2 JP 2754201B2
- Authority
- JP
- Japan
- Prior art keywords
- stink bug
- feeding
- active ingredient
- scarab
- stimulating activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Description
【０００１】[0001]
【技術分野】本発明は、（Ｅ）−３,７,１１,１５−テ
トラメチル−２−ヘキサデセン−１−オールを活性成分
とするハリクチブトカメムシの摂食刺激剤に関する。TECHNICAL FIELD The present invention relates to a feeding stimulant for a stink bug, (E), which comprises (E) -3,7,11,15-tetramethyl-2-hexadecene-1-ol as an active ingredient.
【０００２】[0002]
【背景技術】近年、環境問題への関心の高まりにつれ
て、害虫防除においては殺虫剤散布に代わる防除手段の
開発が社会的な要望になっている。ハリクチブトカメム
シ（学名：Eocanthecona furcellata）は、沖縄や東南
アジアに広く生息する捕食性カメムシであり、鱗翅目、
鞘翅目、膜翅目などの昆虫を捕食する。本種のような捕
食性カメムシは、自然生態系において植食性昆虫の密度
制御要因として重要な機能を果たしていると考えられて
いる。そこで、これらの捕食性カメムシを利用する害虫
防除法の開発について精力的な研究が行われつつある
（四国植防，第28号：103〜108（1993）参照）。2. Description of the Related Art In recent years, as interest in environmental problems has increased, there has been a social demand for the development of control means in place of insecticide spraying in pest control. The stink bug, Eocanthecona furcellata, is a predatory stink bug that widely inhabits Okinawa and Southeast Asia.
Prey on insects such as Coleoptera and Hymenoptera. It is thought that predatory stink bugs like this species play an important role as a factor controlling the density of herbivorous insects in natural ecosystems. Therefore, vigorous research is being conducted on the development of a method for controlling pests using these predatory stink bugs (see Shikoku Planting Control, No. 28: 103-108 (1993)).
【０００３】一方、捕食性カメムシの人工飼育が進めら
れ、その飼料としては、主に生きた鱗翅目昆虫の幼虫や
冷凍保存した幼虫が用いられているが（Rostria，24：1
35〜140（1975）参照）、これらの幼虫の十分な量の確
保が困難なため人工飼料の開発もされつつある（四国植
防，第29号：133〜136（1994）参照）。[0003] On the other hand, artificial breeding of predatory stink bugs has been promoted, and live larvae of live Lepidopteran insects and larvae frozen and stored are mainly used as feeds thereof (Rostria, 24: 1).
35-140 (1975)), and it is difficult to secure a sufficient amount of these larvae, so artificial feeds are being developed (see Shikoku Planting Defense No. 29: 133-136 (1994)).
【０００４】[0004]
【発明の開示】本発明者らは、上記の如き事情に鑑み、
ハリクチブトカメムシの捕食行動の研究を行った結果、
ハスモンヨトウの幼虫からハリクチブトカメムシに摂食
行動（口吻伸長行動）を引き起こす活性成分を見出し、
かかる活性成分を抽出、単離し、この成分と別途化学合
成による化合物とを照合し、ハリクチブトカメムシに摂
食行動を引き起こす活性成分物質を同定した。本発明
は、これらの知見に基づいて完成されたものである。DISCLOSURE OF THE INVENTION In view of the circumstances described above, the present inventors have
As a result of studying the predatory behavior of the beetle,
An active ingredient that causes feeding behavior (mouth prolonging behavior) in the tortoise stink bug from the larva of Spodoptera litura,
The active ingredient was extracted and isolated, and this ingredient was compared with a separately synthesized compound to identify an active ingredient substance that causes feeding behavior in the stink bug, Stinkbug. The present invention has been completed based on these findings.
【０００５】すなわち、本発明は、（Ｅ）−３,７,１
１,１５−テトラメチル−２−ヘキサデセン−１−オー
ルを活性成分として含有することを特徴とする半翅目異
翅亜目カメムシ科に属するハリクチブトカメムシの摂食
刺激剤を提供するものである。That is, the present invention provides (E) -3,7,1
An object of the present invention is to provide a feeding stimulant for a stink bug, Stinkbugs belonging to the family Hemiptera: Heteroptera, Stink bug, comprising 1,15-tetramethyl-2-hexadecene-1-ol as an active ingredient.
【０００６】以下、本発明を詳細に説明する。Hereinafter, the present invention will be described in detail.
【０００７】本発明に係る摂食刺激剤の活性成分物質で
ある（Ｅ）−３,７,１１,１５−テトラメチル−２−ヘ
キサデセン−１−オール（一般名：Ｅ−フィトール）
は、単独でハリクチブトカメムシに対して摂食行動を引
き起こす。この活性成分物質は、そのまま、又はアセト
ンなどの有機溶媒に溶かして適宜濃度の溶液とし、これ
を飼料に塗布しあるいは飼料に添加して液状または固形
状の各種形態の人工飼料とすることができる。The active ingredient substance of the feeding stimulant according to the present invention, (E) -3,7,11,15-tetramethyl-2-hexadecene-1-ol (generic name: E-phytol)
Singly cause feeding behavior on the beetle stink bugs. This active ingredient substance can be used as it is or dissolved in an organic solvent such as acetone to prepare a solution having an appropriate concentration, and this solution can be applied to a feed or added to a feed to prepare various forms of liquid or solid artificial feed. .
【０００８】次に、本発明に係る活性成分物質のハスモ
ンヨトウの幼虫からの単離・同定法並びにその摂食刺激
活性の確認試験について詳細に説明する。Next, a method for isolating and identifying the active ingredient substance of the present invention from the larva of Spodoptera litura and a test for confirming its feeding stimulating activity will be described in detail.
【０００９】[0009]
１．摂食刺激活性の確認法 試料をパスツールピペットにとり空気と混合する。この
試料を混合した気体をカメムシの口先約１から２cmのと
ころに噴出せしめて、カメムシが口吻を伸長するかどう
かにより摂食刺激活性の有無を判断した。 ２．活性成分物質の単離 ハスモンヨトウの幼虫をインゲン豆粉末を主成分とする
人工試料を餌として飼育し、６齢幼虫２０頭を用いてヘ
キサンとアセトンの１：２混合溶媒で３０分間抽出し、
粗抽出物を得た。粗抽出物には明確な摂食刺激活性が認
められた。この粗抽出物をシリカゲルを充填したカラム
クロマトグラフィーにより分画した。溶出溶媒としてヘ
キサン、５％エーテルを含んだヘキサンおよび１５％エ
ーテルを含んだヘキサン溶媒を用いて順次溶出した。得
られた各画分について摂食刺激活性を評価したところ、
１５％エーテル／ヘキサン画分にのみ明確な摂食刺激活
性が認められたが、他の画分には活性がほとんど認めら
れなかった。さらに、この１５％エーテル／ヘキサン画
分を溶媒抽出法を用いて酸性、中性、塩基性画分に分画
したところ、中性画分に強い摂食刺激活性が認められ
た。 ３．活性成分物質の同定 上記摂食刺激活性が認められた中性画分をガスクロマト
グラフ直結型質量分析計（ＧＣ−ＭＳ）を用いて分析し
たところ、炭素数２０個のアルコール化合物を主成分と
していることが明らかにされた。この成分のマススペク
トルを後掲図１に示す。この成分のマススペクトルが別
途化学合成した（Ｅ）−３,７,１１,１５−テトラメチ
ル−２−ヘキサデセン−１−オール（Ｅ−フィトール）
のマススペクトルと全く一致した。さらにガスクロマト
グラフ分析においてこの成分の保持時間が別途化学合成
したＥ−フィトールの保持時間と一致することを確認し
た。この結果から、上記の１５％エーテル／ヘキサン画
分中に含まれる活性成分物質は、Ｅ−フィトールである
と同定された。なお、活性成分物質のハスモンヨトウの
６齢幼虫１頭当たりからの抽出量は約４８０ngであっ
た。 ４．Ｅ−フィトール標品 別途化学合成により得られるフィトールは、Ｅ−及びＺ
−異性体の混合物であるので、これをアセチル化してフ
ィトール アセテートとした後、硝酸銀を含有させたシ
リカゲルカラムクロマトグラフィーを用いて両異性体の
分離・精製を行い、それぞれ加水分解してＥ−及びＺ−
フィトールとしたものを精製して、各標品とした。 ５．摂食刺激活性の確認試験 ハリクチブトカメムシに対する合成Ｅ−及びＺ−フィト
ールと上記１５％エーテル／ヘキサン画分の摂食刺激活
性を上記１．記載の確認法により試験した。この摂食刺
激活性の試験の結果を表１に示す。1. Method for confirming feeding stimulating activity Take a sample in a Pasteur pipette and mix with air. The gas mixed with this sample was ejected at about 1-2 cm from the tip of the stink bug, and the presence or absence of feeding stimulating activity was determined based on whether the stink bug extended its snout. 2. Isolation of Active Ingredient Substance The larva of Spodoptera litura is bred as an artificial sample containing kidney bean powder as a main ingredient, and extracted with a mixed solvent of hexane and acetone for 30 minutes using 20 6th instar larvae,
A crude extract was obtained. A clear feeding stimulating activity was observed in the crude extract. This crude extract was fractionated by column chromatography packed with silica gel. Elution was carried out sequentially using hexane, hexane containing 5% ether and hexane solvent containing 15% ether as elution solvents. When the feeding stimulation activity was evaluated for each of the obtained fractions,
Clear feeding stimulating activity was observed only in the 15% ether / hexane fraction, but almost no activity was observed in the other fractions. Furthermore, when this 15% ether / hexane fraction was fractionated into acidic, neutral and basic fractions using a solvent extraction method, a strong feeding stimulating activity was observed in the neutral fraction. 3. Identification of Active Ingredients When the neutral fraction in which the above-mentioned feeding stimulating activity was observed was analyzed using a gas chromatograph direct-coupled mass spectrometer (GC-MS), an alcohol compound having 20 carbon atoms was the main component. It was revealed. The mass spectrum of this component is shown in FIG. The mass spectrum of this component was separately chemically synthesized to give (E) -3,7,11,15-tetramethyl-2-hexadecene-1-ol (E-phytol).
Completely matched the mass spectrum. Furthermore, it was confirmed by gas chromatographic analysis that the retention time of this component coincided with the retention time of separately synthesized E-phytol. From this result, the active ingredient contained in the 15% ether / hexane fraction was identified as E-phytol. The amount of the active ingredient extracted from the sixth instar larva of the cutworm, Spodoptera litura was about 480 ng. 4. E-Phytol standard Phytol separately obtained by chemical synthesis is E- and Z-
Since it is a mixture of isomers, it is acetylated to phytolacetate, and both isomers are separated and purified using silica gel column chromatography containing silver nitrate, and hydrolyzed to form E- and Z-
Phytol was purified and used as each sample. 5. Confirmation test of feeding stimulating activity The feeding stimulating activity of the synthetic E- and Z-phytol and the 15% ether / hexane fraction on the beetle, Streptomyces stigmata, was measured as described in 1. The test was performed according to the described confirmation method. Table 1 shows the results of the test on the feeding stimulating activity.
【００１０】[0010]
【表１】 上記の試験の結果、（Ｅ）−３,７,１１,１５−テトラ
メチル−２−ヘキサデセン−１−オール（Ｅ−フィトー
ル）に１５％エーテル／ヘキサン画分と同等のハリクチ
ブトカメムシの摂食刺激活性が認められた。[Table 1] As a result of the above test, (E) -3,7,11,15-tetramethyl-2-hexadecene-1-ol (E-phytol) was consumed by a stink bug, which was equivalent to a 15% ether / hexane fraction. Stimulating activity was observed.
【００１１】[0011]
【発明の効果】以上説明したとおり、本発明によりハス
モンヨトウ幼虫に存在する捕食性カメムシに摂食刺激を
示す活性成分物質は（Ｅ）−３,７,１１,１５−テトラ
メチル−２−ヘキサデセン−１−オールであることが同
定された。したがって、当該活性物質は、捕食性カメム
シであるハリクチブトカメムシの人工飼料への添加剤とAs described above, according to the present invention, the active ingredient substance which stimulates the predatory stink bug, which is present in the larvae of Spodoptera litura, to eat (E) -3,7,11,15-tetramethyl-2-hexadecene- It was identified as 1-ol. Therefore, the active substance can be used as an additive to artificial feed for the predatory stink bug, Stinging stink bug
して極めて有用なものである。人It is extremely useful. Man
【図１】 実施例の２で得られた強い摂食刺激活性が
認められた中性画分のマススペクトル図である。FIG. 1 is a mass spectrum diagram of a neutral fraction obtained in Example 2 and having a strong feeding stimulating activity.
Claims (1)
ル−２−ヘキサデセン−１−オールを活性成分として含
有することを特徴とする半翅目異翅亜目カメムシ科に属
するハリクチブトカメムシの摂食刺激剤。The present invention relates to a crabworm belonging to the family Hemiptera Heteroptera, Stinkbug, which comprises (E) -3,7,11,15-tetramethyl-2-hexadecene-1-ol as an active ingredient. Stink bug feeding stimulant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8166642A JP2754201B2 (en) | 1996-06-07 | 1996-06-07 | Feeding stimulants for the scarab stink bug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8166642A JP2754201B2 (en) | 1996-06-07 | 1996-06-07 | Feeding stimulants for the scarab stink bug |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH09322717A JPH09322717A (en) | 1997-12-16 |
| JP2754201B2 true JP2754201B2 (en) | 1998-05-20 |
Family
ID=15835065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8166642A Expired - Lifetime JP2754201B2 (en) | 1996-06-07 | 1996-06-07 | Feeding stimulants for the scarab stink bug |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2754201B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103315155B (en) * | 2013-06-21 | 2014-07-30 | 华南农业大学 | Application of phytol to preparation of pig feed additive and pig feed |
-
1996
- 1996-06-07 JP JP8166642A patent/JP2754201B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH09322717A (en) | 1997-12-16 |
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