JP2742137B2 - Disease control agent and control method for useful plants of Gramineae - Google Patents

Description

Description TECHNICAL FIELD [0001] The present invention relates to a disease control agent and a method for controlling useful plants of the Poaceae family used for microorganisms belonging to the genus Exelohirum.

BACKGROUND OF THE INVENTION In rice cultivation, rice blast is the most frequent and causes a decrease in yield, so it is necessary to spray fungicides once to three or four times a year.

However, in recent years, due to the problem of environmental pollution caused by chemical pesticides, development of a crop disease control agent that does not rely on chemical pesticides and the use thereof have been desired.

Therefore, a method of controlling crop diseases using microorganisms has been considered, but the means can be roughly divided into two. One is a method of inducing rice disease resistance by inoculating a rice cultivar with a non-affinity blast fungus to control blast. As such a method, for example, Tohoku Agricultural Experiment Station Research Report No. 75-27-39
(1987), and the method described in the Japanese Society of Plant Pathology Vol. 56, No. 2, pp. 273-275 (1990). In a similar manner, rice disease resistance is induced by inoculating rice isolates showing weak pathogenicity against rice,
There are also methods to control blast. For example, there is a method described in Kita-Japan Pest Research Bulletin No. 30, pp. 53-55 (1979).

The other is a method for controlling rice blast fungi using a microorganism that exhibits an antagonistic action. As such a method, for example, Japanese Society for Plant Pathology Vol. 58, No. 3, 38
There are a method described on page 0-385 (1992), a method described in JP-A-2-35076, a method described in JP-A-5-65209, and the like.

Problems to be Solved by the Invention However, the above-mentioned method for inducing disease resistance by the incompatible blast fungus has the following problems.
In other words, the property of non-affinity is a relative property between the race of the pathogenic fungus and the cultivar of the crop. May be indicated. For this reason, the method of spraying the rice blast fungus itself on the rice cultivated land may cause blast to varieties showing affinity. In particular, in regions where various different varieties are cultivated, there is a high possibility that blast will occur if there is an affinity rice cultivar. Further, since the non-affinity may be mutated to the affinity, there is a risk that the bacteria exhibiting the affinity may be mixed with the bacteria exhibiting the non-affinity to be sprayed.

In the method for inducing disease resistance by the fungus isolated from rice, Helminthos porium oryzae (Bipolaris or
yzae) has a very high control effect on blast, but it cannot be sprayed on rice cultivated land because it is a causal agent of rice sesame leaf blight.

The above-mentioned method using a microorganism having an antagonistic action against blast fungus generally has a low control effect in many cases. For example, in the method described in the Japanese Society of Plant Pathology, Vol. 58, No. 3, the control effect against blast is only about 50%.
In addition, the microorganism used in this method is often a pathogenic fungus of a useful plant, and has a risk of harming other useful crops. For example, in the method described in JP-A-2-35076, a gladiolus soft-rot bacterial bacterium is used.
In the method described in Japanese Patent Publication No. 9, rice idiot disease fungus is used.

For the above reasons, at present, methods for controlling crop diseases using microorganisms are hardly used despite high social demands.

The present invention has been made in view of the above circumstances, and has as its object to provide means for controlling crop diseases such as blast without affecting the growth of useful plants.

DISCLOSURE OF THE INVENTION As a result of repeated studies on weed pathogens that do not affect the growth of useful plants, the present inventors have found that a strain belonging to the genus Exerohilum spp. The present inventors have found that they exhibit a high control effect, and have completed the present invention.

That is, the first aspect of the present invention is a disease control agent for useful plants of the Gramineae family, which comprises, as an active ingredient, a microorganism belonging to the genus Exelohirum having the ability to control diseases of the Gramineous plants. Here, the microorganisms belonging to the genus Exelohilum include Exelohilum monoceras (Ex
serohilum monoceras), and a more preferable strain is Exerohilum monoceras B026.
Strain, Exero Hilum Monoceras B276, Exero Hilum Monoceras B232, Exero Hilum Monoceras B2
63 strains, Exelohirum monoceras B267, Exerohirum monoceras JTB-159, Exerohirum monoceras JTB-215, Exerohirum monoceras JTB-240
Exelohirum monoceras JTB-264, Exerohirum monoceras JTB-277, Exerohirum monoceras JTB-281, Exerohirum monoceras JTB-6
73 shares, ExeroHirm Monoceras JTB-674, Exerno Hierm Monoceras JTB-675, ExeroHirm Monoceras JTB-676, ExeroHirum Monoceras JTB
-677 strains, Exelohirum monoceras JTB-678 strains, Exerohirum monoceras JTB-679 strains, and Exerohirum monoceras JTB-680.

In addition, the second aspect of the present invention is a method for controlling disease of a useful grass plant, comprising treating a microorganism belonging to the genus Exserohilum having the ability to control disease against a useful grass plant into a useful grass plant. It is.

Furthermore, the third of the present invention is a strain having a disease control ability against useful plants of the Gramineae family, Exerohirum monoceras B026 strain, Exerohirum monoceras B276 strain, Exerohirum monoceras B232 strain, Exerohirum monoceras B263 strain, Exerohirum strain. Monoceras B267 shares,
ExeroHilum Monoceras JTB-159, Exero Hilum Monoceras JTB-215, Exero Hilum Monoceras JTB-240, Exero Hilum Monoceras JTB-264
Strain, ExeroHilum Monoceras JTB-277, Exero Hilum Monoceras JTB-281, Exero Hilum Monoceras JTB-673, Exero Hilum Monoceras JTB-6
74 shares, ExeroHirm Monoceras JTB-675 shares, Exerno Hilum Monoceras JTB-676 shares, Exerno Hirum Monoceras JTB-677 shares, Exero Hirum Monoceras JTB
The strain is selected from the group consisting of -678 strains, Exelohilum monoceras JTB-679 and Exerohilum monoceras JTB-680.

 Hereinafter, the present invention will be described in detail.

The microorganism used in the present invention is not particularly limited as long as it is a microorganism belonging to the genus Exelohirum, but preferably, a microorganism belonging to Exerohilum monoceras is used. Also, preferred strains include Exelohirum monoceras B026 strain, Exerohilum monoceras B276 strain, Exerohilum monoceras B232 strain, Exerohilum monoceras B263 strain, Exerohilum monoceras B267 strain, Exerohilum monoceras JTB-159 Hirumu, Exerohilum monoceras JTB-159 Himuroserox
Monoceras JTB-215 strain, Exerohirum Monoceras JT
B-240 strain, Exerohirum monoceras JTB-264 strain, Exerohirum monoceras JTB-277 strain, Exerohirum monoceras JTB-281 strain, Exerohirum monoceras
JTB-673 strain, Exerohirum monoceras JTB-674 strain,
Exerohirum monoceras JTB-675, Exerohirum monoceras JTB-676, Exerohirum monoceras JTB-677, Exerohirum monoceras JTB-678
Strains, Exerohilum monoceras JTB-679, Exerohilum monoceras JTB-680, and the like.

These strains have been deposited with the National Institute of Advanced Industrial Science and Technology under the following numbers.

Exerohirum monoceras B026 strain: FERM BP-4215 (Original deposit date: March 5, 1993) Exerohirum monoceras B276 strain: FERM BP-4220 (Original deposit date: March 5, 1993) Exerohirum monoceras B232 strain: FERM BP-4217 (Original deposit date: March 5, 1993) Exelohirum monoceras B263 strain: FERM BP-4218 (Original deposit date: March 5, 1993) Exerohilum monoceras B267 strain: FERM BP-4219 (Original Deposit date: March 5, 1993 Exerohirum monoceras JTB-159 strain: FERM BP-4812 (Original deposit date: October 3, 1994) Exelohilum monoceras JTB-215 strain: FERM BP-4814 (Original deposit date) Exelohirum monoceras JTB-240 strain: FERM BP-4814 (Original deposit date: October 3, 1994) Exelohilum monoceras JTB-264 strain: FERM BP-4815 (Original deposit date 1994) October 3) Exero Hilum Monoceras JTB-277 strain: FERM BP-4816 (Original deposit date: October 3, 1994) Exerohirum monoceras JTB-281 strain: FERM BP-4817 (Original deposit date: October 3, 1994) Exerohirum monoceras JTB- 673 strains: FERM BP-4915 (Original deposit date: December 8, 1994) Exerohirum monoceras JTB-674 strain: FERM BP-4916 (Original deposit date: December 8, 1994) Exelohirum monoceras JTB-675 strain : FERM BP-4917 (Original deposit date: December 8, 1994) Exerohirum monoceras JTB-676 strain: FERM BP-4918 (Original deposit date: December 8, 1994) Exerohilum monoceras JTB-677 strain: FERM BP-4919 (Original deposit date: December 8, 1994) Exelohirum monoceras JTB-678 strain: FERM BP-420 (Original deposit date: December 8, 1994) Exerohilum monoceras JTB-679 strain: FERM BP- 4921 (Original deposit date: December 8, 1994) Exelohirum monoceras JTB-680 strain: FERM B P-4922 (Original deposit date: December 8, 1994) These strains were isolated from diseased weeds and their bacteriological properties are as follows.

It is an aerobic bacterium and the colonies are dark green or black on potato sucrose agar medium. Also, white or gray aerial hyphae may be seen. Conidia (conidia) are dark, multivesicular, with 2-8 internal and external septa,
Spindle-shaped, widest at center, narrowing toward both sides. In addition, a navel (hilum) protrudes from the base end. The size of conidia is around 40-150 × 10-25 μm.

Based on the above results, "GRAMINICOLOUS SPECIES" by A.SIVANESAN
OF BIPOLARIS, CURVULARIA, DRECHSLERA, EXSEROHILUM AN
D THEIR TELEOMORPHS ”(Mycological Papers, No.158, p
-21, Nov. 1987.), and "Kamiyama, Tsuda, and Nishihara", "Scientific Names of Hermint Sporium Disease Fungi" (Plant Protection, Vol. 32, No. 9, 361-368, 1978). As a result, the above strain was transformed into Exelohilum monoceras (Exserum) for reasons such as the same colony shape and conidial morphology.
rohilum monoceras).

In addition, the genus name of the genus Exerohirum is
It follows the classification of Tsuda / Nishihara and A.SIVANESAN. LUTTRELL (Revue de Mycologie 41, 271-279,197
7) and ALCORN (Mycotaxon 8, 411-414, 1978) also support this classification. Ellis (Dematioceous
Hyphomycetes, CMI, Kew, 608, 1971) changed the genus Exerohirum and the genus Bipolaris to the genus Drexlera (D
rechslera). However, in recent years, no researchers other than Ellis have found the genus Exerohirum and the genus Bipolaris, so it is considered appropriate to employ the genus Exerohirum.
Since the complete generation of the genus Exerohirum is known to be of the genus Setosphaeria, microorganisms classified as the genus Setosphaeria are also included in the technical scope of the present invention.

No special method is required for culturing the above strain of the genus Exelohirum, and a method similar to that of a known strain belonging to the genus Exerohirum can be used. As the medium, any synthetic medium or natural medium can be used as long as the medium appropriately contains assimilable carbon sources, nitrogen sources, inorganic substances, and necessary growth promoting substances. Specific examples of the medium include an oatmeal sucrose agar medium, an oatmeal agar medium, a potato sucrose agar medium, a V-8 juice agar medium, a Tupec-Docs agar medium, and the like. During culturing, the temperature is 10 to 40 ° C., preferably
It is desirable to maintain the pH at 15-28 ° C, more preferably 20-25 ° C, and the pH at 3-9, preferably 5-8. When culturing is performed for about 7 to 14 days under the above conditions, a sufficient amount of conidia is formed on the surface of the medium.

When the present strain is used in the control method, microbial cells (conidia, ascospores, hyphae, etc.) are used, and preferably, conidia produced by the present strain are used. Conidia can be collected by pouring sterile water onto the microbiota and scraping the surface of the medium with a brush. By this method, 1 × 10 ⁷ to 10 ⁸ conidia can be obtained per petri dish having a diameter of 9 cm.

The disease control agent of the present invention may be used directly as a microorganism, but generally, it is mixed with a solid carrier or a liquid carrier usable for agricultural chemicals, and is used as a solution, wettable powder, powder, granule, emulsion, or oil. , Capsules and the like.

As a microorganism, conidia are usually used. The concentration of conidia is 10 ³ to 10 ⁷ / ml, preferably 10 ⁴ to 10
^It is appropriate to use ⁶ / ml.

When the treatment is carried out in an actual field, it is desirable that the treatment is carried out so that the number of conidia of microorganisms is 10 ⁸ to 10 ¹² , preferably 10 ⁹ to 10 ¹¹ per 10 ares of the field.

Diseases to be controlled according to the present invention include rice blast, rice sesame leaf blight, rice white blight, rice sheath blight, corn sesame leaf blight, corn smut, corn soybean, various types of wheat Diseases of useful grasses such as smut can be mentioned.

Examples (Example 1) B026, B232, B263, B267, B276
Selection of strains etc. Diseased weeds were collected from all over Japan, and pathogenic bacteria were separated from the lesions. Then, the control effect on rice blast was examined by the following method.

Weed pathogens were treated on healthy rice (variety Koshihikari), allowed to stand in a greenhouse for an arbitrary period, and then inoculated with a rice blast fungus exhibiting affinity for the rice. About one month later, the disease index of blast was objectively evaluated, and the most effective Nobie pathogens (B026, B232, B263, B267, B267, B276
Shares, JTB-159, JTB-215, JTB-240, JTB-264
Strains, JTB-277, JTB-281, JTB-673, JTB-674
Strains, JTB-675, JTB-676, JTB-677, JTB-678
Strain, JTB-679 strain and JTB-680 strain).

(Example 2) Rice blast control effect of 19 strains of Nobies pathogen Bacterial seeds of rice (variety Koshihikari) were sown in a pot of 1 / 10,000 are and grown in a greenhouse for about 4 weeks, and then 1%
An aqueous solution of ammonium sulfate was given as fertilizer. About a week later,
The rice was allowed to stand in a greenhouse, and the rice at the 6-leaf stage was used in the control effect test.

19 Nobies pathogens shown in Table 2 were each cultured on oatmeal sucrose agar medium at 25 ° C. for 2 weeks to form conidia. After the conidia were suspended in a 0.02% Tween 20 aqueous solution, a suspension of 10 ⁵ or 10 ⁶ conidia / ml was prepared and used as a treatment liquid. The conidia suspension was sprayed on rice at 1 ml per pot using a sprayer, and then placed in an inoculation box maintained at 25 ° C. and 100% humidity for 20 hours. Each test plot was treated in three pots.

After standing in a greenhouse for 2 days, the rice blast fungus (Pyricularia oryzae race 00
After conidia suspension of 7) (10 ⁵ conidia / ml · 0.02% Tween20 aqueous solution) was sprayed and inoculated (1ml per pot), 25 ° C., 1
Placed in an inoculation box maintained at 00% humidity for 20 hours.

One week after the inoculation, the average number of lesions per leaf of the sixth leaf was examined, and the control value was calculated by the following formula.

As shown in Table 1, all the strains were found to have high blast control effects.

(Example 3) Persistence of rice blast control effect Seeds of rice (variety Koshihikari) were sown in a pot of 1 / 10,000 are and grown at room temperature for about 3 weeks, and then 1%
An aqueous solution of ammonium sulfate was given as fertilizer. The rice was allowed to stand still in a greenhouse for about one week, and the rice at the 4th and 5th leaf stages was used in the control effect test.

The B026 strain of Nobies pathogen was cultured on oatmeal sucrose agar medium for 2 weeks to form conidia. This conidium
After suspending in sterile water containing 0.02% Tween 80, a suspension of 10 ⁵ or 10 ⁶ conidia / ml was prepared and used as a treatment solution. Using a sprayer, this conidia suspension was added to rice per pot.
After spraying 2 ml, they were placed in an inoculation box kept at 25 ° C. and 100% humidity for 18 hours. Each test plot was treated in two pots.

After standing in a greenhouse for 2, 3 or 9 days, the rice blast fungus (Pyricularia) showing affinity for Koshihikari
oryzae Race 013 conidia suspension) (10 conidia ⁵ minutes /ml,0.0
Spray inoculation of 2% Tween80 aqueous solution (1ml per pot)
After that, they were placed in an inoculation box maintained at 25 ° C. and 100% humidity for 18 hours.

Approximately one month after the inoculation, the disease index of blast was subjectively evaluated (evaluated by considering the degree of plant growth, blast lesions, etc., assuming 0 as healthy and 100 as complete withering), and calculating as shown below. The control value was calculated by the formula.

As shown in Table 2, a high control effect on rice blast was observed in the test plots treated at any concentration or regardless of the period after the treatment with the Nobie pathogen.

(Example 4) Control effect of rice blast by seedling box treatment After seeding rice (variety Koshihikari) seeds in a seedling box (12 × 12 × 3 cm), the seedlings are grown in a greenhouse to control 2-3 leaf stage rice. Used for testing. The conidia suspension of JTB-277 strain was sprayed with 10 ⁵ or 10 ⁶ conidia / ml (0.02% Tween20 aqueous solution), and then placed in an inoculation box kept at 25 ° C and 100% humidity.
Left for 0 hours. After standing in a greenhouse for 7 days, five rice plants were transplanted into 1/1000 ares pots in which paddy soil was put into paddy.

Fourteen days after transplantation, the blast fungus (Pyricularia oryzae race 00) showing affinity for Koshihikari, which has reached about the 5 leaf stage.
7) After inoculation, 18 inoculation box kept at 25 ℃ and 100% humidity
Time left. Seven days after inoculation, the average number of lesions per leaf of the fifth leaf was investigated, and the control value was calculated by the following formula. As a result, as shown in Table 3, the seedling box treatment of the disease controlling agent of the present invention resulted in a high control effect on rice blast.

Control value (%) = (1−average number of lesions in treated section / average number of lesions in untreated section) × 100 (Example 5) Control effect on rice blast by foliage treatment 4 m per plot in a field where rice (cultivar Koshihikari) was mechanically transplanted
^A total of twelve test plots, ^two test plots and three test plots (test plots I to IV), were set, and cultivation management was performed as usual. Conidium concentration 10
^The test zone I was sprayed once with a conidium / ml suspension of JTB-678 strain (0.02% Tween20 aqueous solution) at a rate of 100 liters per 10 are using a small pressurized sprayer.
II was sprayed twice, test plot III was sprayed three times, and test plot IV was sprayed four times.

After the flag leaves were completely developed, the disease area ratio of leaf blast in each of the 24 test plots was investigated, and the control value was calculated by the following formula. Two weeks before the harvest, the incidence of all ear blasts in each of the eight test plots was examined by grade, and the control value was calculated by the following generally used formula. As a result, as shown in Table 4, a high control effect on leaf blast and panicle blast was obtained by foliar treatment of the disease controlling agent of the present invention.

Control value of leaf blast = (1-diseased area ratio of treated group / diseased area ratio of untreated section) x 100 Control value of ear blast = (1- diseased degree of treated section ^* / diseased degree of untreated section ^* ) × 100 A: Number of diseased ears of spikelet blast B: Number of diseased ears of branch blasts of 2/3 or more C: Number of diseased ears of branch blasts of 1/3 or more and less than 2/3 D: 1/3 or more of branch blasts Number of diseased ears of rice blast (Example 6) Control effect of rice blast by ear treatment 4 m per plot in a field where rice (cultivar Koshihikari) was mechanically transplanted
Cultivation management was carried out as usual by setting up a test group of ^two or three repetitions. A suspension of JTB-159 strain with a conidium concentration of 10 ⁶ conidia / ml was applied to rice ears once per 10 ares using a small pressurized atomizer.
Sprayed at a rate of 100 liters. Two weeks before the harvest, as in Example 5, the disease incidence of all the ear blasts of the eight strains in each group was investigated by degree and the degree of disease was calculated, and the control value was calculated by the formula shown in Example 5. As a result, as shown in Table 5, a high control effect against ear blast was obtained by the ear treatment of the disease controlling agent of the present invention.

(Example 7) Control effect on rice sesame leaf blight A rice (cultivar Koshihikari) seed was sowed in a pot of 1 / 10,000 are and grown in a greenhouse, and rice at the 4th and 5th leaf stages was used in the control effect test. JTB-679 strain and conidial suspensions JTB-680 strain (0.02% Tween20 aqueous solution) 10 ⁵ or 10 ⁶
After spraying conidia / ml on rice, the rice was placed in an inoculation box maintained at 25 ° C and 100% humidity for 20 hours. After standing in a greenhouse for one day, the rice plant was spray-inoculated with rice sesame leaf blight (Bipolaris oryzae), and further placed in an inoculation box maintained at 25 ° C. and 100% humidity for 20 hours. Seven days after inoculation, the average number of lesions per leaf was examined, and the control value was calculated by the formula shown in Example 4. As shown in Table 6, the disease control agent of the present invention controlled the occurrence of rice sesame leaf blight.

Usage example Formulation example 1 (solution) Conidia of Exerohirum monoceras B026 strain (2 × 10
⁹ ) and Tween80 (4 g) were added to 20 L of sterile water and mixed to prepare a liquid preparation. This solution was sprayed on a 1 R paddy field where blast is predicted to occur.

Formulation Example 2 (Wettable Powder) 10% maltose was suspended clay 1% Water 90% mixture per conidia 1 ml (JTB-680 strain) 10 ⁷ a. After air-drying, the dried product was mixed and pulverized to prepare a wettable powder.

Formulation Example 3 (Wettable powder) Lactose 9%, zeolite 1%, water 90% mixed solution 1m
10 ⁷ conidia (B263 strain) were suspended per l. After air-drying, the dried product was mixed and pulverized to prepare a wettable powder.

Formulation Example 4 (Wettable powder) Conidia (JTB) per 1 g of a mixture of 15% diatomaceous earth, 77% kaolin, and 8% polyoxyethylene alkylphenyl ether
-674 shares) were suspended 10 ⁷ a. After air-drying, the dried product was mixed and pulverized to prepare a wettable powder.

Formulation Example 5 (Wettable powder) diatomaceous earth 33%, carboxymethylcellulose 0.33%, water
66.67% mixture per conidia 1 ml (JTB-159 strain) were suspended 10 ⁷ a. After air-drying, the dried product was mixed and pulverized to prepare a wettable powder.

Formulation Example 6 (powder) 14% hydroxypropyl-β-cyclodextrin,
White carbon 12%, mixture conidia per 1g of clay 74% (B276 strain) 10 ⁷ were mixed. After drying, the powder was uniformly ground to prepare a powder.

Formulation Example 7 (granules) 10 ⁷ conidia (JTB-281 strain) per 1 g of a mixture of 15% β-cyclodextrin, 2% starch, 18% bentonite, 36% calcium carbonate, and 29% water were added and kneaded. Thereafter, granules were prepared by granulating with a granulator and drying.

Formulation Example 8 (emulsion) Polyoxyethylene nonyl phenyl ether ammonium phosphate 18%, polyoxyethylene nonyl phenyl ether 6%, triethyl phosphate 29%, tributyl phosphate
Conidia per 47% of the mixture 1 g (JTB-675 strain) 10 ⁷ were then added to the uniformly suspended was adjusted emulsion.

Formulation Example 9 (oil) Spindle oil 95%, castor oil 4%, conidia in a mixture 1ml of 1% silicone oil (JTB-277 strain) 10 ⁷ were suspended was adjusted oil.

Formulation Example 10 (Dry flowable formulation) 12% sodium alkylbenzene sulfonate, was suspended conidia (JTB-215 strain) 10 ⁷ a in polyethylene glycol ether 88% of the composition 1 ml, was adjusted dry flowable formulation.

Formulation Example 11 (capsule) Conidia (JTB-679) in 1 ml of a mixture of 0.7% sodium alginate, 5% kaolin, 15% glycerin and 79.3% water
Ltd.) 10 ⁷ were suspended to give a capsule form product was dropped into 0.2 molar calcium acetate solution. After chopping this, it was sieved and air-dried to prepare a capsule.

Formulation Example 12 (capsule) Conidia (JTB-678 strain) 1 in 1 ml of a mixture of 0.7% sodium alginate, 5% diatomaceous earth, 15% glycerin and 79.3% water
0 ⁷ was suspended to give a capsule form product was dropped into 0.2 molar calcium chloride solution. After chopping this, it was sieved and air-dried to prepare a capsule.

Effects of the Invention The present invention provides a novel disease control agent and a method for controlling useful plants of the Poaceae family. The controlling agent and the controlling method of the present invention use pathogenic bacteria of weeds, so that there is no concern about environmental pollution,
It does not adversely affect useful plants unlike conventional microorganism control agents. Furthermore, the control agent of the present invention is capable of controlling the disease of useful plants of the Gramineae by one treatment, and also has an effect of controlling the diseases occurring in all the growing seasons of the useful plants of the Gramineae. Therefore, it is extremely useful industrially.

 ──────────────────────────────────────────────────の Continued on the front page Accession number of microorganism FERM BP-4215 Accession number of microorganism FERM BP-4217 Accession number of microorganism FERM BP-4218 Accession number of microorganism FERM BP-4219 Accession number of microorganism FERM BP-4220 Accession of microorganism No. FERM BP-4915 Accession number of microorganisms FERM BP-4916 Accession number of microorganisms FERM BP-4917 Accession number of microorganisms FERM BP-4918 Accession number of microorganisms FERM BP-4919 Accession number of microorganisms FERM BP-4920 Microorganism accession number BP-4921 Microorganism accession number FERM BP-4922 (72) Inventor Fumiki Tsutsumi 6-2 Umegaoka, Aoba-ku, Yokohama-shi, Nippon Tobacco Industry Co., Ltd. Plant Development Research Institute Yokohama Center (72) Inventor Masao Yamada 6-2 Umedaoka, Aoba-ku, Yokohama-shi, Kanagawa Pref. (72) Inventor Tsuneo Ikui 23-8 Higashiharacho, Tsuruoka City, Yamagata Prefecture

Claims (5)

(57) [Claims] 1. A disease controlling agent for useful grasses, comprising a microorganism belonging to the genus Exserohilum having an ability to control diseases of grasses useful as an active ingredient. 2. A microorganism belonging to the genus Exserohilum having an ability to control disease on useful plants of the Poaceae family, wherein the microorganism belongs to the genus Exserohilum monocelas.
ras), the disease controlling agent for useful plants of the grass family according to claim 1, characterized in that it is ras). 3. Exerohilum monoceras (Exserohilu)
m monoceras), Exerohirum monoceras B026
Strain, Exero Hilum Monoceras B276, Exero Hilum Monoceras B232, Exero Hilum Monoceras B2
63 strains, Exelohirum monoceras B267, Exerohirum monoceras JTB-159, Exerohirum monoceras JTB-215, Exerohirum monoceras JTB-240
Exelohirum monoceras JTB-264, Exerohirum monoceras JTB-277, Exerohirum monoceras JTB-281, Exerohirum monoceras JTB-6
73 shares, ExeroHirm Monoceras JTB-674, Exerno Hierm Monoceras JTB-675, ExeroHirm Monoceras JTB-676, ExeroHirum Monoceras JTB
The strain according to claim 2, wherein the strain is selected from the group consisting of -677 strains, Exelohirum monoceras JTB-678 strains, Exerohirum monoceras JTB-679 strains, and Exerohirum monoceras JTB-680 strains. Disease control agent for useful plants of Gramineae. 4. A method for controlling disease of useful grasses, which comprises treating a microorganism belonging to the genus Exserohilum having the ability to control disease with respect to useful plants of grasses. 5. A strain having an ability to control diseases against useful plants of the Poaceae family, wherein the strain is Exelohirum monoceras B026,
Exerohirum monoceras B276 strain,
Monoceras B232 strains, Excelo Hirum Monoceras B263
Strain, Exelohirum monoceras B267, Exerohirum monoceras JTB-159, Exerohirum monoceras JTB-215, Exerohirum monoceras JTB-240
Exelohirum monoceras JTB-264, Exerohirum monoceras JTB-277, Exerohirum monoceras JTB-281, Exerohirum monoceras JTB-6
73 shares, ExeroHirm Monoceras JTB-674, Exerno Hierm Monoceras JTB-675, ExeroHirm Monoceras JTB-676, ExeroHirum Monoceras JTB
-A strain selected from the group consisting of 677 strains, Exelohilum monoceras JTB-678 strain, Exerohilum monoceras JTB-679 strain, and Exerohilum monoceras JTB-680 strain.