JP2728566B2 - How to grow plant seedlings - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for growing plant seedlings. More specifically, shoots obtained by tissue culture of plant shoot tips, petals, etc. often include a phenomenon in which the leaves and stem tissues of a plant become water-immersed (generally referred to as "water-immersed shoots"). This is a major bottleneck for growing large numbers of healthy seedlings. The present invention relates to a method for growing a plant seedling for recovering the water-immersed shoot.

[0002]

2. Description of the Related Art Conventionally, as a method for suppressing the occurrence of water-immersed shoots in tissue culture, an increase in the concentration of agar or gellan gum, which is a support for a tissue culture medium, for example,
"Physiol Plant", Vol. 53, 18
1 to 187, published in 1981), improvement of aeration of the culture vessel (for example, see "Breeding Journal", Vol. Changes (e.g., "Handbook o
f Plant Cell Culture ", Vo
l. 4, 574-611, published in 1986) and the like are reported to be effective. However, in some plants (for example, carnations), the occurrence of water-immersed shoots cannot be completely suppressed by these methods, and it is very difficult to recover the water-immersed shoots once generated. It is.

[0003]

SUMMARY OF THE INVENTION The present inventors have found that water-immersed shoots occur at an extremely high rate in plant tissue culture, and have tried to acclimate these water-immersed shoots, but failed in all cases and died. As a result of diligent studies in an attempt to solve the problems leading to the problem, it was surprisingly found that most of them can be recovered by transplanting the water-immersed shoots into a peptone-containing medium. In addition, it was found that shoots that had recovered from water immersion significantly improved the acclimatization rate, and that healthy plant seedlings were obtained. The present inventors have further studied based on such findings, and as a result, have completed the present invention.

[0004]

The gist of the present invention is to provide a method of growing a plant seedling, which comprises transplanting a water-immersed shoot of a plant produced by tissue culture into a medium containing peptone. The present invention also provides a method for growing a plant seedling, characterized in that the composition of the medium containing peptone is peptone, hyponex, sucrose, and agar. Furthermore, the gist of the present invention is a method for growing a plant seedling, wherein the amount of peptone added to the peptone-containing medium is 1 to 10 g / l.

[0005] Shoots are grown with or without passing through the callus, by cutting out the growing points, petals and the like of the plant and placing it on a tissue culture medium. The shoots thus grown often become water-immersed shoots. By transplanting the water-immersed shoots into a peptone-supplemented medium, normal shoots are recovered at a high rate. The peptone-supplemented medium used in the method of the present invention is obtained by adding peptone to a generally known various tissue culture medium in an amount of 1 to 10 g / p.
It is adjusted so as to be 1.

[0006]

EXAMPLES Hereinafter, the method of the present invention will be described in more detail based on examples, but the method of the present invention is not limited thereto. Example 1, Example of carnation shoot apex culture 1) Generation of water soaked shoots by shoot apex culture A well-growing mother strain (cultivar: Silver) cultivated in a greenhouse
y Pink, Albisole, and Odeon), ears including the tip of the stem were collected, and the length was 5 to 10 c.
m. Next, under a stereoscopic microscope (magnification: 10 to 40 times) in a clean bench, young leaves or primordium were sequentially removed from the outside with a scalpel to expose the shoot apical growth points. When the growing point is exposed, the shoot apical tissue containing about 2 to 4 juvenile discs and having a height of about 0.3 to 0.5 mm is separated,
The medium was placed on an H1 medium (medium composition is as described below).

[0007] Note) Hyponex is the United States The Hyponex
CO. , INC. Made. After placing the shoot apex tissue, at a temperature of 25 ° C,
Illuminance of about 2,000 lux. For 12 hours per day. After a few months, a shoot about 2 cm high was obtained. The occurrence rate of water-immersed shoots in the obtained shoots varied between 26.9% and 84.6% depending on the variety.

2) Recovery of water immersion chute The water immersion chute obtained in 1) is cut from the base and has a length of 1.5 to 2.
It was set to 0 cm, and it was transplanted to a peptone-containing medium (medium composition is as described below) in a tube bottle. Note) Peptone (Peptone) used is US DI
FCO LABORATORIES product name “BA
CTO-PEPTONE ". The results of an investigation of the recovery of water-immersed shoots 30 days after transplanting to a medium containing peptone were added.

The results are shown in Table 1.

As is evident from Table 1, more than 80% of the water-immersed shoots were recovered by transplantation into the medium containing peptone.

Example 2, Example of petal culture of carnation 1) Generation of water-immersed shoots by petal culture A well-growing parent strain (variety: Scanni) cultivated in a greenhouse
From b), buds before flowering were collected. Next, after immersing the buds in sodium hypochlorite solution (effective chlorine 1%) for 5 minutes,
The surfaces were sterilized by washing with sterile water. Peel off the sepals in the clean bench, peel off the petals one by one, and
rage & Skoog medium (hereinafter simply referred to as “MS
Abbreviated as “medium”. The medium composition is as follows. ). After placing the petals, the temperature was 25 ° C. and the illuminance was about 2,000 lux.
For 12 hours per day. Three months later,
A shoot about 2 cm high was obtained. All the obtained shoots were water-immersed shoots.

2) Recovery of water-immersed chute The water-immersed chute obtained in 1) is cut from the base to have a length of 1.5 to 2.0 cm, and is cut into a peptone-containing medium (other than peptone) in a tube bottle. (See Table 2 above). The amount of peptone added to the peptone-added medium was 1 g / l in the non-added section, 2 g / l in the added section, 3 g / l in the peptone-added section.
1 and 5 g / l were respectively adjusted. The results of investigating the recovery of water-immersed shoots two months after transplanting them into each of the 25 peptone-supplemented cultures in each plot

The results are shown in Table 2.

As is clear from Table 2, recovery of the water-immersed shoots was observed by transplantation into the medium containing peptone. The amount of added peptone showed the highest recovery rate in the 3 g / l-added group, but there was a tendency for the growth degree to slightly decrease when the added amount of peptone was further increased.

The recovered shoots obtained in Examples 1 and 2 are cut from the base and are 1.5 to 2.0 cm long.
And transplanted it into a rooting medium (medium composition is as follows) in a tube bottle. Four weeks after transplantation to the rooting medium, the rooting seedlings were removed from the tube bottle and the agar was washed off. This young plant is planted in a pot containing perlite prepared in advance,
Acclimation was performed in a greenhouse at 25 to 35 ° C. under high humidity and light shielding. As a result, the acclimation success rate was 90% or more. On the other hand, when the water-immersed shoots were transplanted to a rooting medium without recovery, and the obtained rooting seedlings were planted in pots containing pearlite prepared in the same manner, all could not be acclimated.

[0012]

As described above, a water-immersed shoot generated when a plant is tissue-cultured can be restored to a normal shoot by transplanting it into a peptone-added medium. The shoots recovered in this way have a high success rate of subsequent acclimation, and are extremely effective for growing a large number of healthy seedlings of plants.

[Table 1]

[Table 2]

Claims (3)

(57) [Claims] 1. A method for growing a plant seedling, comprising transplanting a water-immersed shoot of a plant produced by tissue culture into a medium containing peptone. 2. The method for growing a plant seedling according to claim 1, wherein the composition of the peptone-containing medium is peptone, hyponex, sucrose, or agar. 3. A method for growing a plant seedling, wherein the amount of peptone added to the peptone-containing medium according to claim 1 or 2 is 1 to 10 g / l.