JP2684059B2 - Bacteria liquid preparation equipment - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a bacterial liquid preparation instrument that is widely used in the fields of medicine, biology, biochemistry and the like.

[Background Art] In various technical fields dealing with bacteria, it is necessary to quantitatively inoculate the same amount of bacterial cells in order to test the properties of the bacteria or to test the susceptibility of the bacteria to a drug. However, when the bacterial cells of bacteria are directly used, the amount of the bacterial cells varies, so that the bacterial cells are often used as a bacterial solution.

Therefore, in this case, the bacterial liquid must have the bacterial cells uniformly dispersed in the liquid.

By the way, among the bacteria, mycobacteria include tubercle bacillus as a typical one, but since the bacteria easily adhere to each other and have a property of being difficult to separate when adhered, they are uniformly dispersed in a liquid. Has difficult characteristics.

Therefore, when preparing a bacterial suspension of such bacteria, unlike the preparation of bacterial suspensions of other general bacteria, physical force is not applied to the bacterial cells placed in the liquid to disperse the bacterial cells. However, a homogeneous bacterial solution cannot be prepared.

Conventionally, a glass homogenizer method, a hand shaking method, etc. have been used for the preparation of the bacterial solution in such a case, but each of these conventional methods has some problems.

That is, the glass homogenizer method is composed of a test tube whose inside is ground glass, and an outer cylinder which is inserted into this test tube and whose outside is ground glass, and puts the bacterial mass in the above test tube, and then Insert the above cylinder,
This is a method of crushing the bacterial mass by rubbing the ground glass parts of both, but this method is extremely undesirable in hygiene management because bacteria may jump out of the test tube because of the opening operation.

Next, the hand gesture method is 50 ml containing a 7-8 mm crystal ball.
The method is to put the bacterial mass in a thick round-bottomed flask, shake it by hand, and crush the bacterial mass by rotating the crystal ball, but since this method uses a round-bottomed flask, the flask is , In addition to having an unstable shape for standing, the bacteria may fly out of the flask due to the opening operation, which is also extremely undesirable in hygiene control.

DISCLOSURE OF THE INVENTION The present invention provides a device for preparing a bacterial liquid, which solves the drawbacks of the conventional methods and enables homogeneous dispersion of bacterial cells.

That is, the present invention provides an instrument for preparing a bacterial solution, which comprises a test tube with a cap that contains a small amount of distilled water and a plurality of spherical solid materials. Hereinafter, the present invention will be described in detail.

The material of the test tube used in the device for preparing a bacterial liquid of the present invention is not specified, but it is recommended to use a synthetic resin material, preferably polypropylene.

The cap may be attached to the test tube in any form as long as it can seal the test tube. For example, the plug type and the lid type are preferably applied, but the screw type and the push cap type are also applicable to the lid type.

The material of the spherical solid material is not specified, but either an organic material such as a synthetic resin or an inorganic material such as glass or crystal can be used. Preferably, glass balls are used. A plurality of spherical solid materials are used, for example, 3 to 6, and the size thereof is such that they can be contacted and rotated in a test tube.

Normally, in a test tube with an inner diameter of 14 mm to 17 mm, the ball size is 2 mm to 7 mm in diameter.

A small amount of distilled water is stored, and usually 0.1 ml to 1 ml is preferable for a test tube having an inner diameter of 14 mm to 17 mm.

The device for preparing a bacterial solution according to the present invention is used, for example, as follows.

Remove the cap and insert the fungus mass into the test tube using a platinum loop. At this time, the bacterial mass is made to reach the sterile distilled water contained therein, and the bacterial mass is separated from the platinum loop. Attach the cap to the test tube, seal the test tube, and mix with a test tube mixer for 20 to 30 seconds. By this mixing, the bacterial mass is dispersed by the movement of the glass beads in the test tube. By adding 3 to 5 ml of sterilized distilled water and mixing again lightly, a safe and quick homogeneous bacterial solution can be obtained.

The device for preparing a bacterial liquid according to the present invention has the following characteristic advantages.

1. Since the bacterial solution is prepared in a closed system in a test tube, the bacteria do not fly off when the bacterial solution is prepared, which is safe for hygiene management.

2. Since the instrument can be sterilized in advance, the procedure for preparing the bacterial solution can be performed easily.

3. The operation is simpler than the conventional method, and a homogeneous bacterial solution can be obtained in a short time.

4. The use of polypropylene material for test tubes and caps provides the following additional advantages.

 There is little danger because it does not break even if dropped.

 Disposable products are provided.

Since it can be sterilized by autoclave at the time of product manufacturing, there is no residue of toxic substances and no influence on the sample.

[Brief description of the drawings]

FIG. 1 is an explanatory view showing an embodiment of a device for preparing a bacterial liquid of the present invention, 1 is a test tube containing a ball 3 and distilled water 4, and 2 is a cap.

Claims (2)

(57) [Claims] 1. A device for preparing a bacterial solution comprising a test tube with a cap, which contains a small amount of distilled water and a plurality of spherical solid materials. 2. The instrument for preparing a bacterial solution according to claim 1, wherein the capped test tube is made of polypropylene material.