JP2650222B2 - Disease control agent by Rhizoctonia - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an agent for controlling disease caused by Rhizoctonia bacterium, and more particularly to an agent effective for controlling diseases caused by brown patches and large patches on turf, in addition to diseases on vegetables.

[0002]

2. Description of the Related Art Various pesticides are used to control plant pests. For example, thiuram, captan, thiophanate methyl, benomyl, mepronil, iprodione, chloroneb, chloroneb, turf phos-methyl, and isoprothiolane furtranil for large patches of turf. , Captan, iprodione and the like are known.

[0003]

However, all of these pesticides are chemicals, and these pesticides are more or less toxic to humans or animals due to their use or residual concentrations (respiratory disorders, skin disorders, germs, etc.). (E.g., cancer, teratogenicity) and fish toxicity in wastewater. However, the use of pesticides has been limited due to environmental pollution due to residual toxicity. In particular, the regulations of local governments are becoming stricter, especially for golf courses.

[0004] For controlling plant diseases using microorganisms,
In recent years, in addition to traditional fermentation-type applications, the use of antagonistic microorganisms that directly act on pests has recently been used, but their practical fields are still limited.

[0005] Further, for example, a product of a bacterium belonging to Bacillus thuringiensis is used as an insecticide for insects of the order Lepidoptera, but its use is restricted due to toxicity to silkworms.

The present invention provides a culture solution of a specific microorganism whose use has not been known so far as a pesticidal agent.

[0007]

DISCLOSURE OF THE INVENTION The present inventors have discovered Bacillus sphaericus T-1 bacteria earlier and used the spore-containing culture to prevent the generation of flies and foul odors generated in the dung of livestock. Due to the effect of
No. 00182 (Japanese Patent Publication No. 3-20) filed an application as a feed additive for livestock, and Japanese Patent Application No. 60-46082 (Japanese Patent Publication No. 63-404) applied as a nematicide, Thereafter, various experiments were conducted on the effective use of the Bacillus, and as a result, it was found that the Bacillus had an effective control action against various diseases of turf.

That is, the present invention is a disease control agent comprising a spore-containing culture of Bacillus sphaericus T-1.

The Bacillus sphaericus T-1 bacterium used in the present invention has been deposited with the Institute of Microbial Industry and Technology as No. 7820 (FERM P-7820). No. 59-200182 (Japanese Patent Publication No. 3-20).

The term "culture" according to the present invention means a culture itself obtained by culturing Bacillus sphaericus T-1 in a liquid medium, or a concentrate or dried product thereof, wherein the culture is a culture containing cells. It means both the liquid and the culture liquid from which the cells have been removed by filtration or the like.

The above-mentioned liquid medium is usually a liquid medium in which bacteria of the genus Bacillus grows, and is not particularly limited. Usually, broth or tryptoisobuillon is suitable.

The culture of the present invention is harmless to humans and animals that can be used as feed for livestock such as poultry raising and pig raising, as described in the specification of Japanese Patent Publication No. 3-20 / 1991. It is effective in controlling pests. For example, turf Rhizoc
Effective for controlling brown patches and Rhizoctonia large patches by tonia solani Kuehn.

It is also effective for the application of PCNB, whose use is being regulated recently due to its teratogenicity and environmental pollution. For example, it is effective for the above club brassicae and potato black bruises. It is considered to be particularly effective against diseases such as wilt, root rot, black bruise, bud wilt, stem rot and the like of seeds and standing seedlings.

The culture of the present invention is applied as it is, or a liquid obtained by appropriately diluting a culture solution or a dry powder thereof, and is usually applied to soil or has a cell count of 10 ⁷ to ^{10 7} / m 2.
l of the culture is diluted 10-1000-fold and applied by spraying or spraying. The amount of application is 10 to 100 ml / m ² in terms of a stock solution. For example, a 10 to 50-fold diluted solution is applied at a rate of 1 l / m ² and applied several times as needed. It is also effective to soak the roots in a 10-fold diluted solution at the time of planting of seedlings.

In addition, it is also possible to spray directly on the leaves of the plant, but in this case, it is preferable to spray water to wash the surface of the leaves.

The culture of the present invention can be used alone or in combination with various fertilizers.

[0017]

The mechanism of action of the culture of the present invention for controlling various pests and insect pests is not always clear. However, Bacillus sp. Bacteria are known, and it is presumed that the Bacillus sphaericus T-1 bacterium of the present invention also has such properties. However, unlike Bacillus thuringiensis, there is no harm to the silkworm, and there is no fish poison. It has been confirmed that there is no. It is harmless to humans, mammals and birds.
It has been confirmed that there is no harm to earthworms such as daphnia and earthworms, which are the feeds for appreciation fish, and their usefulness is very remarkable.

[0018]

EXAMPLES Next, the effects of the present invention will be described with reference to examples, but the present invention is not limited to the following examples.

The spore-containing culture solution of Bacillus sphaericus T-1 of the present invention (hereinafter simply referred to as culture solution) used in the following examples of controlling various plants was produced by the method according to Example 1. .

Example 1 (Preparation of culture solution) Bacillus sphaericus T-1 was cultivated in a slope on a normal agar culture medium to obtain a preservation strain, and one platinum loop was added to the broth (pH 7.2) 10 ml from the slope culture. Inoculate the
After culturing at 35 ° C for 48 hours, the same normal broth 200m as above
transferred to l incubated for 48 hours under the same conditions, the culture was further transferred to the same nutrient broth 4000ml and above, 35 ° C. in a small jar fermenter, for 48 hours under aeration culture, bacterial number 10 ⁸ -
^{A 10} / ml culture was obtained.

Example 2 (Control of turf blight on turf of golf course) Opening in Gunma Prefecture 1
At a golf course in 2010, a 9-hole course brown patch and Rhizoctonia large patch were controlled by spraying 1 l / m ² using a 50-fold dilution of a culture solution (the number of bacteria was about 10 ⁷ / ml).

For green, in May-June 1993
When sprayed three times every 10 days and three times in August-September, no disease was found in either Koryo or Bent. The other 9-hole control greens became ill and were treated with pesticide treatment.

When spot treatment was performed only on the diseased site except for green, the spread of the disease was stopped in about 10 days after the treatment, and the elongation of the leaves was observed in about 10% of the diseased area.
He recovered in 15 days, with the remaining 10% restraining the spread of the disease but not recovering.

[0024]

Industrial Applicability The culture of the present invention has a wide-ranging control effect on the disease caused by Rhizoctonia on turf and vegetables, is harmless to humans and animals, has no toxicity to fish, and has no risk of environmental pollution.

Claims (1)

(57) [Claims] 1. Bacillus sphlicus (Bacillus sph)
aericus) An agent for controlling disease caused by Rhizoctonia bacterium comprising a culture containing spores of T-1 bacteria.