JP2632636B2 - Disinfection method and apparatus using electrolytically activated water - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and an apparatus for disinfecting pathogenic microorganisms such as viruses and bacteria.

[0002]

2. Description of the Related Art Virucidal and bacterial methods have been known for a long time to prevent infection by viruses and bacteria. Physical methods such as heat treatment, ultraviolet irradiation,
There are methods such as irradiation of radiation, and as a chemical method,
A method using a heavy metal compounds and a halogen compound as a disinfectant.

[0003]

Among these methods, the method by heat treatment is a method of sterilizing with high-pressure steam. However, since high-temperature, high-pressure steam is used, there is a danger of burns if the equipment is mishandled. There is. Another problem is that the material of the device is deteriorated by heating.

[0004] The method using ultraviolet irradiation has a problem in that only the surface to be irradiated is disinfected and the back surface is not disinfected. The method using radiation irradiation has problems in that the equipment is not widely used and handling is difficult.

The chemical method is a method for killing viruses or disinfecting by utilizing the protein denaturing effect of a heavy metal compound or a halogen-based disinfectant, and is mainly used in medical institutions. The problem with this method is that when a disinfectant is administered to blood, body fluids, pus, sputum or other lumps, the surface of the lumps is denatured with protein and has a disinfection effect, but the disinfectant does not penetrate into the lumps,
The inside cannot be disinfected. Furthermore, since heavy metal compounds and halogen compounds are pollutants, the use of these disinfectants should be suppressed from the viewpoint of environmental protection.

[0006] Therefore for the present invention is to solve such problems, handling is easy, provided without adversely affecting the etc. human body and the environment, and sterilization, the sterilizing method and apparatus of superior pathogenic microorganisms virucidal effect The purpose is to do.

[0007]

In the present invention, a strongly acidic liquid obtained by electrolyzing water such as tap water, well water and natural water is used as a disinfectant for pathogenic microorganisms. This liquid
An oxidation-reduction potential obtained from the anode chamber is 900 mV or more by adding an electrolyte such as salt to water and performing electrolysis in an electrolytic cell having a diaphragm separating the cathode and the anode.
H is 3 or less strongly acidic water (hereinafter, referred to as electrolytically activated water). Electrolytically activated water can be continuously produced by performing electrolysis while supplying water containing an electrolyte such as salt to an electrolytic cell partitioned by a diaphragm.
The pH can be adjusted by the amount of water or electrolyte supplied and the voltage used for electrolysis.

[0008]

The above-mentioned electrolytically activated water has an excellent effect of killing viruses, bacteria, fungi and the like. In addition, since the composition is water, it can penetrate and dissolve blood, body fluids, pus, sputum, and other lumps into the interior, so unlike conventional heavy metal or halogen-based disinfectants, it completely disinfects the interior of the lumps it can.
The above points were confirmed by experiments. The experiment was performed using various electrolytically activated waters having different pHs from the oxidation-reduction potential, and it has been confirmed that when the oxidation-reduction potential is 900 mV or more and the pH is 3 or less, virus killing and bactericidal effects are obtained. The results of experiments using electrolytically activated water having an oxidation-reduction potential of 1000 mV and a pH of 2.6 will be described below.

Virus killing effect of electrolytically activated water (1-1) Effect on herpes simplex virus type 1 herpes simplex virus type 1 (herpes simpl)
ex virus type 1: hereinafter referred to as HSV-1 virus).

In order to examine the virucidal effect of electrolytically active water, it is sufficient to add electrolytically active water to the virus solution and see how the number of viruses contained in the virus solution changes. In general, the number of viruses contained in a virus solution is determined as follows. Cells that are susceptible to infection with the virus under investigation are selected, and various virus solutions with different dilutions are applied to the cells to determine the dilution at which the probability that the virus will infect the cells is 50%. That is, a virus solution as it is, a stock solution diluted 10 times,
Make 100-fold dilutions, 1000-fold dilutions, etc., and check whether the virus infects cells. Check the probability that the virus will infect cells by 50%.
To find the dilution. The dilution at which the virus has a 50% probability of infecting cells is referred to as a 50% tissue culture cell infectious titer (hereinafter, infectious titer). For example, the probability of infection when diluted 1000-fold if 50% are infectious titer 10 ^3. The probability of transmission of one virus is almost 50
%, The infection titer is 10 ³ in the above example, which is 10 3.
Will be 00 times the virus viral solution was diluted're one, the number of viruses in the original virus stock of pre-dilution is 10 ^3. That is, the infectious titer may be considered to represent the number of viruses, and if the infectious titer is determined by the above method, the number of viruses contained in the original virus stock solution can be determined.

CV-1 cells (monkey liver cells) are HVS
-1 Very susceptible to virus. Then, in order to examine the virucidal effect of the electrolytically activated water on the HVS-1 virus, CVS-1 cells were used and the HVS-1 virus was obtained by the above method.
The virus infectivity was determined.

(A) Virucidal effect when the concentration of electroactive water is 90% 0.1 ml of HVS-1 virus solution and 0.9 m of electroactive water
1 at room temperature to make the concentration of electrolysis activated water 90%,
After 1, 3, 5, 10 and 15 minutes, the infectivity titer of the HVS-1 virus was measured, and the virucidal effect of electrolytically activated water was examined. In this experiment, 10 6.3 / ml of virus were used.
The squared number is included. (Infectious titer / ml = 10 ^6.3 ) The HVS-1 virus solution before the treatment was used. The “electrolytically active water concentration” is the ratio of “electrolytically active water” to “total amount of virus solution and electrolytically active water”. In this experiment, as described above, 0.1 ml of virus solution and 0.9 ml of electrolytically active water were used. And the concentration of electrolytically activated water is 90%.

FIG. 1 shows the experimental results. The horizontal axis in FIG. 1 shows the processing time (minutes), and the vertical axis shows the infectious titer per ml as a logarithmic value. In the experiment, three types of electrolytically activated water having different days after generation were used. Ex shown in FIG.
p. 1, Exp. 2 and Exp. 3 is generated by husband and wife 3
These are the results using electrolytically activated water after 5 days, 5 days, and 7 days. Exp. According to the result of No. 1, H
In the VS-1 virus solution, there were 10 6.3 viruses, and when the electrolyzed water 3 days after the generation was added, the infectivity of the HVS-1 virus was 1 minute later. It is so severe that the virus is completely inactivated. Exp. 2, the infectious titer decreases to some extent one minute after mixing. Not as much as one. However, it drops considerably after 3 minutes. Exp. No. 3, the infectious titer was 1 minute after Exp. 2, but the infectious titer decreases slowly thereafter and does not decrease so much after 15 minutes. From the above, it can be seen that as the number of days after the generation of electrolytically activated water elapses, the virucidal effect decreases.

Regarding the effect of the concentration of electrolytically active water on the virucidal effect and the toxicity of electrolytically active water, the virus fluid is suspended in a body fluid having a chemical composition close to that of the body fluid. Thus, an HSV-1 virus solution having an infectious value / ml of 10 ^6.3 ,
Three days after the production, the electroactive water is mixed with the electroactive water so that the concentration of the electroactive water becomes 90%, 75%, 50%, and 25%. It was measured using cells. An experiment was also conducted to examine the toxicity to CV-1 cells at that time. The experimental results are shown in Table 1 below.

[0015]

[Table 1]

From the results shown in Table 1, it can be seen that the 90% concentration has a virucidal effect, while the other concentrations have no virucidal effect. It can also be seen that any concentration of electrolyzed water is not toxic to CV-1 cells. The reason why the virus-killing effect is not observed in the electrolyzed water having a concentration of 75% or less is considered that the oxidation-reduction potential of the electrolyzed water is neutralized by the liquid in which the virus is floating.

(C) Influence of infectious titer of virus solution Next, in order to see what effect the infectious titer of the HSV-1 virus solution has on the virucidal effect of electrolytically activated water, various infectious titers were examined. An experiment was performed using a virus solution having a valency. The used electrolyzed activated water is 90% on the 5th day after generation
Concentration. This was reacted with HSV-1 virus solutions having various infectious titers for 3 minutes at room temperature, and the remaining H
The infectious titer per 1 ml of the SV-1 virus solution was measured.
The results are shown in Table 2 below. “<(10 ⁰⁾ ” in Table 2
Indicates that the infection titer / ml is less than 1.

[0018]

[Table 2]

From the results shown in Table 2, it can be seen that the HSV-1 virus having an infectious titer of 10 ^5.3 / ml or less was completely inactivated.

(1-2) Effect on Other Viruses In order to examine whether there is a virus-killing effect on viruses other than the HSV-1 virus, electrolytic activity was measured against the five viruses shown in Table 3 below. Water was applied. Among these viruses, the mouse hepatitis virus (hepatiti)
sB virus: HBV causes serum hepatitis and the AIDS virus (human immunodef
Because of the cause of AIDS, the virus is also feared by healthcare professionals and the general public. One day after the generation, the electroactive water was mixed with each virus solution to a concentration of 90%, and after mixing, 3 minutes later,
The virucidal effect of the electrolytically activated water was examined by measuring the infectious titer of the remaining virus solution. The results are shown in Table 3 below.
As shown in Table 2, no infectious titer was observed for any of the viruses, indicating that these viruses were completely inactivated by treatment with electrolytically activated water.

[0021]

[Table 3]

(2) Bactericidal Effect of Electrolytic Activated Water The effects of electrolytic activated water on viruses have been described above. Here, the effects of electrolytic activated water on bacteria and fungi will be described.

For bacteria and fungi, the bactericidal effect of the electrolytically activated water was investigated. Three bacteria and one fungus were investigated. These bacteria are in a broth culture (nutrient b).
roth). Each of the bacterial liquids is 0.
1 ml and 0.9 ml of electrolytically active water were mixed to obtain a 90% concentration of electrolytically active water, treated at room temperature for 3 minutes, and the number of remaining bacteria was examined by colony formation. The results are shown in Table 4 below, and it was confirmed that all the bacteria were completely inactivated by the 90% concentration of the electrolyzed water.

[0024]

[Table 4]

Next, 90% concentration of old electrolytically activated water on the 12th day after production was mixed with the four kinds of bacterial liquids described in Table 4,
, 5 minutes and 10 minutes, the number of remaining bacteria was examined. The result is shown in FIG. The horizontal axis in FIG. 2 represents the processing time (minutes), and the vertical axis represents the number of bacteria. From FIG. 2, it can be seen that even with the old electrolytically activated water, a considerable bactericidal effect is exhibited with a treatment time of 1 minute for any of the bacteria.

Penetration Effect of Electrolytic Activated Water When a human blood clot (about 5 mm in diameter) and gauze to which blood adhered were immersed in electrolytic activated water, the blood clot and blood adhering to the gauze were completely dissolved after 30 minutes.

(4) Conclusion From the above description, it has been clarified that electrolytically activated water has an excellent viricidal and bactericidal effect on various viruses, bacteria and fungi. In addition, the penetration effect of the electrolytically activated water was also confirmed, and it was found that unlike the conventional heavy metal or halogen-based disinfectants, the use of electrolytically activated water can completely disinfect the inside of the lump.

Electrolytically activated water has a pH value over a period of days after generation.
Does not change, but the oxidation-reduction potential decreases. And, as mentioned above, the virus killing effect and the bactericidal effect of the electrolytically activated water vary depending on the concentration of the electrolytically activated water, the number of days after generation, and the like.
It is considered that the virus killing effect and the bactericidal effect of the electrolytically activated water are based not only on the acidity and alkalinity of the electrolytically activated water but also on the high oxidation-reduction potential. Electrolytically activated water has been confirmed to be non-toxic, but when electrolyzed water comes in contact with body fluids, including viruses and sterilization, its oxidation-reduction potential and pH rapidly change, and in a short time ordinary water is not used. It is thought to be to return to. As described above, the electrolytically activated water has no adverse effect on the human body and the environment, and is easy to handle.

[0029]

Embodiments of the present invention will be described below.

(First Embodiment) The first embodiment relates to a method of disinfecting all articles used in medical and dental medical activities.

[0031] The blood or body fluid of the patient often adheres to the articles used in the medical practice, and the HB is transmitted through the blood or body fluid.
There is a risk that the virus, HC virus, AIDS virus, ATL virus, etc. will infect healthcare workers and other patients. In addition, pus adhering due to treatment of suppuration patients may contain MRSA (methicillin-resistant Staphylococcus aureus), which is feared by medical workers and patients in recent years.

The mode of the first embodiment will be described below for each object to be disinfected. (1) Scalpels, scissors, tweezers and other solids Immerse in a water tank filled with electrolyzed water for at least 5 minutes. (2) White robes, gloves, masks, gauze, absorbent cotton, bandages and other cloth-like materials Immerse in a water bath soaked with electrolytically activated water for at least 15 minutes. (3) Those containing water, such as blood, body fluids, pus, and sputum.
/ 10 or less, and soak for at least 30 minutes.

(Second Embodiment) The second embodiment relates to a method for disinfecting pathogenic microorganisms contained in blood and body fluid splashed by a turbine used for dental treatment.

When cutting teeth in dental treatment, a turbine is used. FIG. 3 shows a perspective view of the tip of the turbine. When the operation switch of the turbine is turned on, air compressed by a compressor (not shown) passes through the air supply pipe 11,
The air is supplied to the blades in the head 16 from the air supply pipe tip 15, rotates the rotor 14, and the cutting tool 17 attached to the tip of the rotor 14 cuts teeth.

When cutting a tooth using a turbine, the affected part around the tooth being cut often bleeds, and the blood, saliva and the like are brought into an aerosol state by the cutting tool 17 and the rotor 14 rotating at high speed. Scatter. In a conventional turbine, a water supply port 13 shown in FIG.
Water is sprayed onto the rotor 14 to suppress the scattering of blood and saliva of the patient.

However, when the operation of the turbine is stopped, the bleeding blood and saliva in the oral cavity of the patient flow back into the water supply pipe 12 from the water inlet 13 and the blood and saliva of the patient before flowing back to the next patient are jetted. Will be done. That is, conventional turbines carry pathogenic microorganisms that are transmitted through blood or body fluids. Also, considering that patients receiving dental treatment have a higher antibody titer of HB virus than ordinary people, it cannot be denied that they are infected with pathogenic microorganisms mediated by blood or body fluids of patients.

Therefore, in the second embodiment, virus contained in blood or saliva of a patient in the turbine is used by using electrolytically activated water having a viricidal effect instead of water jetted from the water inlet 13 of the turbine. Inactivates.

(Third Embodiment) A third embodiment relates to a method of disinfecting a site infected with a pathogenic microorganism in dentistry. Many dental diseases are due to bacterial or fungal infections, and oral mucosal patients are due to viral, bacterial or fungal infections. Therefore, to treat these diseases, it is effective to remove viruses, bacteria or fungi that are the source of infection.

Therefore, in the third embodiment, the infectious source is inactivated by spraying electrolytically active water to the infected site by utilizing the viricidal, bactericidal, and fungicidal effects of the electrolytically active water. This is a method of cleaning the site.

(Fourth Embodiment) The fourth embodiment relates to a method for disinfecting contamination of food or tableware by pathogenic microorganisms.

By utilizing the bactericidal effect and the virucidal effect of the electrolytically activated water, foods or dishes contaminated with putrefactive bacteria or pathogenic microorganisms are disinfected with the electrolytically activated water. Electrolytically activated water is harmless to the human body, and is also suitable for disinfecting tableware.

In the fourth embodiment, in the case of food, a food which is 1/10 or less of the weight of the electrolytically active water to be used is mixed with the electrolytically active water for at least 10 minutes, or washed with the electrolytically active water. How to In the case of tableware, it is a method of immersing in a water tank filled with electrolytically active water for at least 5 minutes or washing the tableware with electrolytically active water.

[0043] The Other Embodiments Other embodiments, pacifier, which put in the mouth, such as toothbrushes, tables, desks, which touches by hand, such as chairs, one body, such as toilet seat This is a method of disinfecting the parts that come into contact with electrolytically activated water.

[0044]

EFFECTS OF THE INVENTION The electrolytically activated water has an excellent virucidal effect,
Since it has a bactericidal effect, it is suitable for disinfecting pathogenic microorganisms.
In addition, since the composition of electrolytically activated water is water, blood,
It dissolves lumps such as body fluids, pus, and sputum, and can completely disinfect the interior of the lumps, unlike conventional heavy metal or halogen-based germicides, which disinfect only the surface due to protein denaturation. Electrolytically activated water returns to normal water in a short time due to contact with viruses, bacteria and other foreign substances, so that it has no adverse effect on the human body and environment and is easy to handle.

[Brief description of the drawings]

1 is a diagram showing an inactivation effect of simple herpes virus type 1 by the electrolytic activated water (HSV-1 virus).

FIG. 2 is a diagram showing the inactivating action of bacteria and fungi by electrolytically activated water.

FIG. 3 is a perspective view of a dental turbine tip.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 11 Air supply pipe 12 Water supply pipe 13 Water inlet 14 Rotor 15 Air supply pipe tip 16 Head 17 Cutting tool

 ────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Soichiro Kuniyoshi 2-26 Grace Takahashi B-102 14-26 Aramakihonzawa, Aoba-ku, Sendai City, Miyagi Prefecture (56) References JP-A-4-339934 (JP, A)

Claims (2)

(57) [Claims] 1. A direct-current voltage is applied between the cathode and anode electrodes of an electrolytic cell having an ion-permeable diaphragm partitioning between an anode and a cathode, into which a cathode electrode is inserted, and an anode chamber, into which an anode electrode is inserted. , well water, when performing electrolysis of water such as natural water, it is produced in the anode chamber, and the pH in the oxidation-reduction potential 900mV or more using the 3 following electrolytic activated water, adhesion pathogenic microorganisms is attached A method for disinfecting a body and a medical device or an adherent and a medical device to which pathogenic microorganisms may adhere. 2. A dental medical turbine comprising a cutting tool for shaving teeth and a means for jetting cleaning water near a rotor for rotating the cutting tool, wherein the disinfection using electrolytically activated water according to claim 1 as said cleaning water. apparatus.