JP2612001B2 - Granules containing useful intestinal bacteria and method for producing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a granule containing useful intestinal bacteria which is effective when applied to health foods or medicines, and a method for producing the same.

[Conventional technology]

Numerous bacteria are resident in the human intestine, and their activities are involved in the chemical reactions of substances in the human intestinal tract, and have a significant effect on intestinal function and, consequently, human health. well known.

Among these bacteria, bihidobacteria and lactic acid bacteria do not have a harmful effect by themselves, and when they proliferate in the intestine, the proliferation of other harmful bacteria is suppressed. Since the generation of harmful products due to abnormal intestinal fermentation or the like is prevented, it is considered that it is desirable to grow the above-mentioned bacteria and lactic acid bacteria in the intestine for maintaining health.

By the way, humans are supplied with amino sugars such as N-acetylglucosamine from mother's milk, a component called so-called behidas factor, during infancy. As a result, more than 90% of the endogenous bacteria are occupied by these useful bacteria,
It is said that extremely good bacterial flora is formed in the intestine.

However, when the supply of bihidas factor decreases with the growth and cessation of ingestion of breast milk, the useful intestinal bacteria decrease and the bacterial flora deteriorates. Attempts have been made to improve the flora, and tablets and lactic acid bacteria-containing tablets and foods and beverages containing bihidobacteria for the purpose are commercially available.

[Problems to be solved by the invention]

Generally, when useful enteric bacteria such as lactic acid bacteria and Viridus bacteria are orally administered in tablets and foods and drinks, a considerable amount of viable bacteria will be killed by gastric juice. Concentration must be included, for example,
It is said that a concentration of about 1 × 10 ¹⁰ viable bacteria per gram is required for behydobacteria.

However, the conventional lactic acid bacterium tablets and behidas bacterium-containing beverages have a drawback that it is difficult to stably store high-concentration viable bacteria.

That is, in the case of tablets, there is a problem that most viable bacteria are killed because strong pressure or heat is applied in the tableting process.

On the other hand, in the case of foods and drinks containing water, the live bacteria in the food and drink are activated by the water, and there is a problem that they die when there is no nutrient. Foods and drinks containing nutrients of live bacteria are also provided, but this reduces the concentration of live bacteria in food and drink, and kills live bacteria due to lactic acid produced by the activity of live bacteria In any case, it is difficult to store a high concentration of viable bacteria in these foods and drinks for a long period of time.

Further, powdered foods and foods called so-called soft granules are provided as fixed foods containing useful intestinal bacteria, but powdered foods are not suitable as foods. There is a disadvantage that it is inconvenient to eat casually.

In addition, soft granules are a mixture of powdered bacteria and a granular taste, etc., but if it is going to contain viable bacteria at a high concentration, it will inevitably be close to powder. It has the same disadvantages as the powdered food.

In addition, since these fixed foods are all subjected to pressure or heat during the manufacturing process, or water is used as a solvent for the binder, there is a problem that the concentration of viable bacteria is reduced as in the case of the tablets. is there.

In this way, a product containing useful intestinal bacteria that can store viable bacteria at a high concentration and is easy to take has not yet been provided.

The present invention has been made in view of the above problems, and an object of the present invention is to provide useful enteric bacteria-containing granules effective for use in health foods or medicines and a method for producing the same.

[Means for solving the problem]

The granules containing useful enteric bacteria according to the present invention are obtained by granulating useful enteric bacteria or a powder carrying the useful enteric bacteria using shellac as a binder.

Examples of useful intestinal bacteria that can be used in the present invention include B.
bifidum, B.longum, B.adolescentis, B.breve, B.infantis
Bifidobacterium; L. acidophilus,
Lacto culm bacteria such as L.lactis, L.casei, L.bulgaricus (Lacto
bacillus); or Streptococcus lactis.

These bacteria are used as fungal powder or powder supported on skim milk powder, starch, etc., and the bacteria contained in the granules may be one kind or a mixture of many kinds. Is also good.

When shellac is used as a binder when granulating the above-mentioned bacteria-containing granules, the storage stability of the bacteria is specifically improved, which has been found by the present inventors, and is an edible binder. When using natural gums such as zein, cyclodextrin, starch, fructooligosaccharides, xanthan gum, locust bean gum, and polyvinyl acetate resin as conventionally used, the reason is unknown, but useful intestinal bacteria Storage stability is significantly reduced.

Further, when granules are granulated using the above shellac as a binder, if water is contained in a solution of the binder, the bacteria may be killed.In the present invention, an anhydrous ethanol solution of shellac is used. Therefore, there is no fear of killing the bacteria.

The concentration of bacteria contained in the granules is, for example, 5 × 10 ⁹ or more, preferably 10 ¹⁰
Pcs / g or more.

As the granulation method, it is necessary to adopt a method that does not excessively apply pressure or heat, and specifically, an extrusion granulation method using a porous cylinder, a rolling granulation method, a fluidized granulation method, or the like is preferable. .

The granules containing useful intestinal bacteria obtained in this way are hardly subjected to pressure, heat and moisture during granulation, so that they contain a high concentration of viable bacteria and, furthermore, use shellac as a binder to produce granules. The storage stability of the bacteria becomes extremely good.

In addition, other nutrients, flavors, flavors, components useful for the propagation of bacteria and the like may be added as long as the bacterial concentration in the granules does not fall below about 10 ¹⁰ cells / g. A useful intestinal bacteria-containing granule useful as a health food that can be easily eaten is obtained.

Furthermore, the intestinal useful bacteria-containing granules of the present invention can also be used as pharmaceuticals such as intestinal medicine or diarrhea treatment.For example, the granules obtained by the above-mentioned production method are charged into a fluid coating apparatus, and cellulose acetate phthalate, hydroxy Propyl methylcellulose phthalate,
By applying an enteric coating such as carboxymethylethylcellulose on the surface of the granules to prevent the killing of viable bacteria by stomach acid, it is possible to obtain a drug that effectively exerts its medicinal effect in the intestine.

〔The invention's effect〕

The intestinal useful bacteria-containing granules of the present invention granulated using shellac dissolved in absolute ethanol contain a high concentration of viable bacteria, and furthermore, by using shellac as a binder, the storage stability of viable bacteria is improved. The properties are extremely good.

In addition, since it is formed into granules, it can be easily taken.

Therefore, the intestinal useful bacteria-containing granules of the present invention are extremely useful as health foods or pharmaceuticals, and the purpose of maintaining healthy by growing intestinal useful bacteria such as bihidobacteria and lactic acid bacteria in the intestine is unfortunately regrettable. Can be demonstrated.

Hereinafter, the present invention will be described in detail with reference to examples,
The present invention is not limited to the following embodiment.

[Example 1] Commercially available Vihidas powder "Mill Freeze" (trade name: manufactured by Japan Freeze Drying Laboratory Co., Ltd.) was used. Moreover, "A-30" (trade name: manufactured by Gifu Shellac Mfg. Co., Ltd.) was used as shellac, and an anhydrous ethanol solution having a shellac concentration of 8% was prepared.

10 ml of an anhydrous ethanol solution of shellac was added to 100 g of the above “Mill Freeze” and granulated using a cylindrical extrusion granulator (pore size: 24 mesh).
MINI "(trade name: manufactured by Freund Corporation)
Drying was performed at room temperature for 20 minutes to obtain good granules.

The number of viable bacteria in 1 g of granules calculated from the above mixing ratio and the number of viable bacteria actually measured by analyzing the granules immediately after granulation are shown in Table 1 below. In addition, the obtained granules are packaged in a sealed state by 2 g each, and
Table 1 also shows the time-dependent changes in the number of viable bacteria (actually measured values) when left in a room at ℃.

[Example 2] An 80% ethanol solution having a shellac concentration of 8% was used instead of the anhydrous ethanol solution having a shellac concentration of 8%.
Granulation was performed in the same manner as in Example 1 to obtain granules.

The number of viable bacteria in 1 g of granules calculated from the above mixing ratio and the number of viable bacteria actually measured by analyzing the granules immediately after granulation are shown in Table 1 below. In addition, the obtained granules are packaged in a sealed state by 2 g each, and
Table 1 also shows the time-dependent changes in the number of viable bacteria (actually measured values) when left in a room at ℃.

[Comparative Example] The viable cell count in 1 g of granules obtained by granulating in the same manner as in Example 1 except that the binder and the solvent were changed, and the granules obtained by analyzing the granules immediately after granulation were measured. The number of bacteria is shown in Table 2 below. In addition, the obtained granules are packaged in a sealed state by 2 g each, and
Table 2 also shows the time-dependent changes in the number of viable bacteria (actually measured values) when left in a room at ℃.

[Example 3] With respect to 9 parts by weight of the Bididas bacterial powder "Mill Freeze",
Skim milk 1 part by weight / Wax coated L-ascorbic acid 0.2 part by weight / Sucrose powder 2 parts by weight / Sitevia 0.0
Good granules were obtained by granulating in the same manner as in Example 1 except that a powder obtained by mixing 1 part by weight of a flavorant consisting of 2 parts by weight was used.

Table 3 below shows the viable cell count in 1 g of the granules calculated from the above mixing ratio and the viable cell count actually measured by analyzing the granules immediately after granulation. In addition, the obtained granules are divided into 2 g each in a sealed state,
Viable cell count when left indoors at ℃ and 35 ℃ (actual measurement)
Table 3 also shows the change with time.

Example 4 To 20 g of commercially available lactic acid bacterium powder (L. bulgaricus), 2 ml of an anhydrous ethanol solution having a shellac (“A-30”) concentration of 8% was added, and the above-mentioned cylindrical extrusion granulator (pore size: 24 mesh) was used. It was granulated and immediately dried under reduced pressure at room temperature for 16 hours to obtain good granules.

The number of viable bacteria in 1 g of granules calculated from the above mixing ratio and the number of viable bacteria measured by analyzing the granules immediately after granulation are shown in Table 4 below. In addition, the obtained granules are divided into 2 g each in a sealed state,
Viable cell count when left indoors at ℃ and 35 ℃ (actual measurement)
Table 4 also shows the time-dependent changes of.

Example 5 Bifidobacterium powder "Mill Freeze" used in Example 1
150 g of the fluidized-bed granulator “FL-MINI” (trade name: manufactured by Freund Corporation) is charged with an anhydrous ethanol solution having a shellac (“A-30”) concentration of 15% while flowing at room temperature.
While spraying 56 ml, the mixture was granulated and dried to obtain good granules.

The number of viable bacteria in 1 g of granules calculated from the above mixing ratio and the number of viable bacteria actually measured by analyzing the granules immediately after granulation are shown in Table 5 below. In addition, the obtained granules are divided into 2 g each in a sealed state,
Viable cell count when placed in a room at ℃ and 35 ℃ (actual measurement)
Table 5 also shows the time-dependent changes of.

As described above, it was found that the intestinal useful bacteria-containing granules of Examples 1 to 5 contained a high concentration of viable bacteria, and also had extremely good storage stability of viable bacteria.

Claims (5)

(57) [Claims] 1. Granules containing useful enteric bacteria, which are obtained by granulating useful enteric bacteria or a powder carrying said useful enteric bacteria using shellac as a binder. 2. The granule containing useful enteric bacteria according to claim 1, wherein the useful enteric bacteria is a bihidobacterium. 3. The granule containing useful enteric bacteria according to claim 2, wherein 1 g of the granule contains approximately 1 × 10 ¹⁰ viridus bacteria. 4. An intestinal useful bacterium characterized in that granules are obtained by using an anhydrous ethanol solution of shellac when granulating the useful enteric bacteria or the powder carrying the useful enteric bacteria to obtain granules. Production method of contained granules. 5. The method according to claim 4, wherein the granulation is performed by an extrusion granulation method, a tumbling granulation method or a fluidized granulation method.
The method for producing intestinal useful bacteria-containing granules according to the above item.