JP2514665B2 - Filtration jig - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention is used for holding a filter and performing a trapping treatment, a staining treatment, a washing treatment, etc. on a material such as cells injected on the filter. Filter jig.

[Conventional technology]

The applicant has previously applied for a filter holder (Japanese Patent Application No. 61-250188) in order to perform a rapid capture treatment, staining treatment, washing treatment, etc. on a large amount of material such as cells. The filtration of the solution, cleaning liquid, etc. by the holder of the filter body 10 is performed by applying the suction force from the suction device to the check valve provided on the lower surface of the filter body by applying the solution or the like injected on the upper surface of the filter body to the check valve. It is achieved by suction force.

[Problems to be solved by the invention]

The above-mentioned conventional technique has an excellent effect that a large amount of material can be rapidly processed, but depending on the material, it may be deformed by suction force for solution filtration, or a material having high adhesiveness such as sputum. In the case of dyeing or washing treatment, there is a problem that it is strongly sucked into the fine pores of the filter, or the solution or washing solution is rapidly sucked and easily dries and sticks to the filter surface. It was easy.

The present invention has been conceived in view of the above-mentioned problems, and it is possible to rapidly process a large amount of material without deforming a material that is easily deformed or a material having high adhesiveness, or fixing the material to the filter surface. The object is to provide a filtration jig that can be used.

[Means for solving problems]

Therefore, it is composed of the assembly body 1, the main body 2, the pressing body 3, the elevating body 4, the nozzle body 5, and the water absorbing member 6.

The assembly body 1 mounts a filter body 10 composed of a frame body 8 and a filter piece 9 stretched on the frame body 8 on the upper end surface of an inner collar piece 7 formed on the upper end portion of the inner peripheral surface. This is the mounting surface 11.

The main body 2 has a substantially cylindrical shape in which the outer peripheral surface is attached to the inner peripheral surface of the assembly body 1, the pressing body 3 has a substantially cylindrical shape having an injection opening 12, and the filter body 10 is placed on the mounting surface in the lower part. 11 It is pressed on and pinched. The elevating body 4 is assembled between the upper end surface of the main body 2 and the inner collar piece 7 of the assembling body 1 so as to be vertically movable.

The upper end of the nozzle body 5 is assembled to the lifting body 4 and is located inside the main body 2. The water absorbing member 6 is attached to the upper part of the elevating body 4, and when the elevating body 4 rises, it comes into close contact with the filter piece 9.

[Action]

 A description will be given with reference to FIGS. 1 and 2.

FIG. 1 shows a state other than the filtration state (suction state). In this state, the assembly of the water absorbing member 6, the elevating body 4, and the nozzle body 5 is lowered by its own weight, and the water absorbing member 6 and the filter piece 9 are separated from each other. Therefore, the solution in the suction opening 12 is not filtered.

FIG. 2 shows the filtration state. In this case, the suction device is attached to the nozzle opening 21 of the nozzle body 5, and the nozzle body 5 is pushed upward by this assembling force so that the water absorbing member 6 comes into close contact with the lower surface of the filter piece 9.
Since the water absorbing member 6 has good water absorption and water permeability, the solution on the filter body 10 is filtered by the filter element 9 due to the capillary phenomenon.
Of the water absorbing member 6 and is absorbed by the water absorbing member 6. And
The solution absorbed by the water absorbing member 6 reaches the lower portion of the water absorbing member 6, and the solution reaching the lower portion is absorbed by the suction force from the suction device assembled to the nozzle body 5.

That is, the solution in the injection opening 12 is not filtered by the suction force from the suction device, but is filtered by the capillary action of the water absorbing member 6. Then, the solution that has been filtered and absorbed by the water absorbing member 6 is sucked by the suction device.

Of course, when the material is not deformed or adhered to the filter piece 9, the suction force from the suction device, the suction force from the suction device, or the like in the state where the water absorbing member 6 is in close contact with the lower surface of the filter piece 9, It is also possible to filter by a pressure from a pressure device that can be attached to the pressing body 3.
Depending on the material, the capillarity, the suction force of the suction device, and the pressing force of the pressurizer can be used together.

The attachment and detachment of the filter body 10 can be achieved by releasing the assembling of the pressing body 3 and the assembling body 1. Also, the water absorbing member 6 is attached and detached by the main body 2
After removing the assembling of the assembly body 1 and the assembly body 1, the lifting body 4 is taken out from the assembly body 1.

〔Example〕

 One embodiment is shown in FIG. 1 to FIG.

This holds a circular filter body 10 by itself as shown in FIG. 6, and the main body 2, the assembly body 1, and the pressing body 3 are substantially cylindrical. Body 2 and assembly 1,
The assembling of the pressing body 3 and the assembling body 1 is achieved by screwing.

The water absorbing member 6 is formed of a water absorbing piece 14 made of a material having good water absorbing and water retaining properties, and a suction body 16 having an upper surface assembled to the lower surface of the water absorbing piece 14 and having a communication hole 15 in the vertical direction. In this case, the solution or the like absorbed by the water absorbing piece 14 is sucked through the communication hole 15 of the suction body 16 by the suction force of the suction device. The assembly of the water absorbing member 6 is
This is achieved by placing the water absorbing piece 14 peripheral end portion in a peripheral groove formed on the upper end peripheral portion of the lifting body 4 and inserting the pressing member 19 into the concave groove.

A packing 20 is provided on the peripheral end of the filter body 10 to prevent the solution or the like on the filter piece 9 from leaking.

The lower end opening of the pressing body 3 communicates with the lower end of the injection opening 12, and a detection hole 17 for detecting the liquid level is formed.

Since the liquid level in the detection hole 17 is naturally at the same level as the liquid level in the injection opening 12, it is possible to know the liquid level in the injection opening 12 by detecting the liquid level in the detection hole 17. I can. Various methods are conceivable for detecting the liquid surface, and it is possible to detect the liquid surface by the light reflected on the liquid surface in the injection opening 12. However, if the detection hole 17 is provided as in the present embodiment, more accurate detection is possible. Liquid level detection is possible, and depending on the material, such accurate liquid level detection is necessary.

For example, in the case of dyeing or washing highly sticky material such as sputum, if time passes after filtering until there is no solution on the filter, the highly sticky material dries and sticks to the filter. In that case, when the liquid level is detected and the solution reaches, for example, about 2 to 3 mm on the filter, the material is dried and the filter is injected by injecting the next solution or cleaning solution. It is possible to solve the problem of sticking to.

Of course, this liquid level can be detected visually, but when processing a large amount of material, it is necessary to perform automatic detection by a level sensor or the like. By doing so, it is possible to automatically inject or stop the infusion of a solution or the like.

Further, a flow piece 18 floating on the liquid surface is provided in the detection hole 17, and the liquid surface can be detected by, for example, whether or not the light emitted from the level sensor is blocked by the float piece 18. It is possible to further facilitate surface detection.

FIG. 5 shows a plan view of the lifting body 4, in which many vertical grooves 13 are formed on the circumferential surface of the lifting body 4, and by doing so, The contact area is small so that it can be moved up and down easily.

FIG. 7 shows another embodiment. The water absorbing member 6 here is
Only a slightly thicker water-absorbing piece 14 made of a material having a good water-absorbing property and a water-retaining property is constituted.

In this embodiment, similarly to the embodiment shown in FIGS. 1 to 5, a step 22 is formed on the inner peripheral surface of the upper end portion of the pressing body 3, and a mounting portion 23 (here, a thread) is formed on the outer peripheral surface of the upper end portion. Is forming. The step 22 is for assembling a filter piece 24 having pores coarser than the fine holes of the filter piece 9,
With this, multiple filtration is performed. Also, the mounting part
A cap 25 is attached to 23 to accelerate the filtration of the solution or the like in the injection opening 12 by the capillarity by the pressurizing force from the pressurizing device, or to filter by the pressurizing force itself.

8 and 9 show still another embodiment. This is a filter jig for one sample, which holds a relatively large rectangular filter body 10, as in the previous two embodiments. In order to filter the solution on the large filter body 10, a plurality of (here, two) nozzle bodies 5 are provided.

10 and 11 show yet another embodiment. This holds a filter body 10 in which a plurality of (four in this case) circular filter pieces 9 are stretched around a frame body 8. A plurality of independent injection openings 12 are formed in the pressing body 3, and the nozzle body 5 and other members necessary for filtration are provided below each of them. The filtration jig shown in this embodiment is for multiple samples, which has the advantage that different types of materials can be processed at once.

The assembling of the assembling body 1 and the pressing body 3 is achieved by engagement. In this case, if both are hinge-coupled, the assembling operation can be easily performed.

〔The invention's effect〕

As described above, according to the present invention, the water absorbing member 6 is brought into contact with the lower surface of the filter piece 9 at the time of filtration so that the solution is filtered by the capillary phenomenon. It will not be deformed by being suddenly sucked on the piece 9, and even if it is a material with high adhesiveness such as sputum, it will be strongly sucked on the filter piece 9 or it will be dried.
It does not stick to the filter piece 9. Therefore, the filtration jig according to the present invention exhibits an excellent effect that it is possible to quickly and surely process such a material that is easily deformed or a material having high adhesiveness.

[Brief description of drawings]

FIG. 1 is a sectional view showing a non-filtered state in one embodiment, FIG. 2 is a sectional view showing its filtered state, FIG. 3 is its plan view, FIG. 4 is a front view, and FIG. FIG. 6 is a plan view of the body 4, and FIG. 6 is a plan view showing the filter body 10 held by the body 4. FIG. 7 is a sectional view showing another embodiment. FIG. 8 is a sectional view showing still another embodiment, and FIG. 9 is a plan view thereof. FIG. 10 is a sectional view showing still another embodiment, and FIG. 11 is a plan view thereof. Explanation of symbols 1: Assembly body, 2: Main body, 3: Pressing body, 4: Lifting body, 5: Nozzle body, 6: Water absorbing member, 7: Inner flange piece, 8: Frame body, 9: Filter piece, 10 : Filter body, 11: Mounting surface, 12: Injection opening, 13: Vertical groove, 14: Water absorption piece, 15: Communication hole, 16: Suction body, 17: Detection hole, 18: Float piece, 19: Pressing material , 20: Packing, 21: Nozzle mouth, 22: Step, 23: Mounting part, 24: Filter piece, 25: Cap.

Claims (8)

(57) [Claims] 1. A frame body (8) and a filter piece (9) stretched on the frame body (8) at an upper end surface of an inner flange piece (7) formed at an upper end portion of an inner peripheral surface of a cylindrical body. Filter body consisting of and (10)
An assembly (1) serving as a mounting surface (11) for mounting, an approximately cylindrical main body (2) having an outer peripheral surface assembled to an inner peripheral surface of the assembly (1), and an injection opening A pressing body (3) having a substantially tubular shape having (12) and pressing the filter body (10) onto the mounting surface (11) at the lower part, and an upper end surface of the main body (2) and the assembly. Attached body (1) Inner collar piece (7)
An ascending / descending body (4) that is vertically movable between the upper and lower parts, a nozzle body (5) whose upper end is assembled to the ascending / descending body (4) and is located in the main body (2), A filtration jig comprising a water-absorbing member (6) having a good water-absorbing property and a water-retaining property, which is attached to the upper part of the body (4) and comes into close contact with the filter piece (9) when the elevating body (4) rises. 2. The filtering jig according to claim 1, wherein a large number of vertical grooves (13) are formed on the outer peripheral surface of the elevating body (4). 3. The filtration jig according to claim 1, wherein the water absorbing member (6) is formed of a water absorbing piece (14) made of a material having good water absorbing and water retaining properties. 4. The water absorbing member (6) is provided with a water absorbing piece (14) made of a material having good water absorbing and water retaining properties, and a lower surface of the water absorbing piece (14) is assembled with an upper surface thereof and a communication hole (15) is provided in a vertical direction. The filter jig according to claim 1 or 2, wherein the filter jig is formed with the suction body (16). 5. The invention according to claim 1, wherein the injection opening (12) is a single piece, and a plurality of the nozzle bodies (5) are provided below the injection opening (12). The filtration jig according to item 3 or item 4. 6. The method according to claim 1, wherein a plurality of the injection openings (12) are formed and the nozzle body (5) is provided below each injection opening (12). The filtration jig according to item 3 or item 4. 7. A detection hole (17) for detecting a liquid level in the injection opening (12) by connecting a lower end opening of the pressing body (3) to the lower end of the injection opening (12). ) Is formed, the filter jig according to claim 1, claim 2, claim 3, claim 4, claim 5, or claim 6. 8. The filtration jig according to claim 7, wherein a float piece (18) floating on a liquid surface is provided in the detection hole (17).