JP2512426B2 - Polymer for liquid separation - Google Patents
Polymer for liquid separationInfo
- Publication number
- JP2512426B2 JP2512426B2 JP61040898A JP4089886A JP2512426B2 JP 2512426 B2 JP2512426 B2 JP 2512426B2 JP 61040898 A JP61040898 A JP 61040898A JP 4089886 A JP4089886 A JP 4089886A JP 2512426 B2 JP2512426 B2 JP 2512426B2
- Authority
- JP
- Japan
- Prior art keywords
- polymer
- liquid separating
- separating agent
- liquid
- copolymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は液体分離剤に関する。詳しくは、血清又は血
漿の分離のように、液体中に共存する特定の成分と他の
成分とをその比重差を利用して分離する際、両成分の中
間の比重を有し、両成分の間に間隔を形成することによ
って、両成分の分離操作を容易にする目的に使用される
液体分離用ポリマーを含有する液体分離剤に関する。TECHNICAL FIELD The present invention relates to a liquid separating agent. Specifically, when separating a specific component and another component coexisting in a liquid by utilizing the difference in specific gravity, such as separation of serum or plasma, it has an intermediate specific gravity of both components, The present invention relates to a liquid separating agent containing a liquid separating polymer used for the purpose of facilitating the separation operation of both components by forming a space therebetween.
従来、この種の液体分離剤用のポリマーとしては、シ
リコーン油、塩素化ポリブテン油或いはα−オレフィン
とマレイン酸ジエステルとの共重合物などが知られてい
る。これらに疎水性微粉末シリカや疎水性スメクタイト
粘土などの無機微粉末からなるチクソトロピー化剤を分
離混練することにより適度な強度のゲル状物としたもの
が、遠心分離操作時には流動性を示すが、その他の場合
は非流動性状態を保つような液体分離剤として用いられ
ている。Heretofore, as a polymer for this type of liquid separating agent, silicone oil, chlorinated polybutene oil, a copolymer of an α-olefin and a maleic acid diester, and the like have been known. What is made into a gel of appropriate strength by separating and kneading a thixotropic agent consisting of an inorganic fine powder such as hydrophobic fine powder silica and hydrophobic smectite clay into these shows fluidity during centrifugation operation, In other cases, it is used as a liquid separating agent that maintains a non-flowing state.
しかし、ポリマーとしてシリコーン油を主体とした液
体分離剤は、分離器内壁の撥水性汚染や電離放射線によ
る滅菌工程での劣化或いは高価であるので汎用の使い捨
て製品は経済的に不向きであるなどの欠点を有してい
る。However, the liquid separating agent mainly composed of silicone oil as a polymer is water-repellent on the inner wall of the separator or deteriorates in the sterilization process due to ionizing radiation or is expensive, so that general-purpose disposable products are economically unsuitable. have.
また、塩素化ポリブテン油あるいはα−オレフィンと
マレイン酸ジエステルとの共重合物を主体とした液体分
離剤では、チクソトロピー化剤との親和性が不充分であ
ることから、加熱処理や長期保存或いは遠心分離操作な
どの条件下で相分離が生じ、ゲルの物性変化による隔壁
機能の低下や油分の分離による分離成分への汚染などを
起こしやすいといった欠点や、耐熱性が不充分なので、
調製、充填、滅菌工程での分解物の発生と、これによる
分離成分への汚染を起こしやすいといった欠点を有して
いる。Further, a liquid separating agent composed mainly of a chlorinated polybutene oil or a copolymer of an α-olefin and a maleic acid diester has insufficient affinity with a thixotropic agent, and therefore heat treatment or long-term storage or centrifugation. Phase separation occurs under conditions such as separation operation, and there are drawbacks such as deterioration of the partition function due to changes in the physical properties of the gel and contamination of separated components due to oil separation, and insufficient heat resistance.
It has the drawback that decomposition products are generated during the preparation, filling, and sterilization steps and that the separated components are easily contaminated.
本発明の目的は、これらの欠点の無い、実用的に優れ
たポリマーを含有する液体分離剤を提供することにあ
る。An object of the present invention is to provide a liquid separating agent containing a polymer that is practically excellent and does not have these drawbacks.
本発明者らは、かかる目的を達成するための鋭意検討
を重ねた結果、特定の範囲のスチレン類とα，β−不飽
和ジカルボン酸ジエステルとの共重合物が、所期の目的
の液体分離剤用のポリマーとして極めて優れていること
を見出して本発明に到達した。The present inventors have conducted extensive studies to achieve such an object, and as a result, a copolymer of styrenes in a specific range and an α, β-unsaturated dicarboxylic acid diester was found to be a target liquid separation product. The present invention has been achieved by finding that they are extremely excellent as polymers for agents.
即ち、本発明の要旨は、一般式： で示される構造を有する、スチレン類とα，β−不飽和
ジカルボン酸ジエステルとの液状の共重合体を含有する
液体分離剤に存する。That is, the gist of the present invention is the general formula: A liquid separating agent containing a liquid copolymer of styrene and an α, β-unsaturated dicarboxylic acid diester having a structure of
以下、本発明について詳細に説明する。 Hereinafter, the present invention will be described in detail.
本発明の液体分離剤用のポリマーは、分離されるべき
液体の粘度や液体中の二成分の比重およびその比重差に
合わせて、幅広い範囲で自由に粘度および比重を微調整
したポリマーを容易に製造することができる。The polymer for the liquid separating agent of the present invention can easily be a polymer whose viscosity and specific gravity are finely adjusted in a wide range in accordance with the viscosity of the liquid to be separated, the specific gravity of the two components in the liquid and the difference in their specific gravities. It can be manufactured.
即ち、比重の調整には、炭化水素基R3，R4の炭素数の
調整及び共重合モル比ｍの調整により行なうことができ
る。一般にR3、R4の合計炭素数を小さくすること、また
共重合比ｍを大きくすることによって比重を高めること
ができる。また、粘度の調整には、重合度ｎの調整が最
も効果的といえる。That is, the specific gravity can be adjusted by adjusting the carbon number of the hydrocarbon groups R 3 and R 4 and adjusting the copolymerization molar ratio m. Generally, the specific gravity can be increased by decreasing the total carbon number of R 3 and R 4 and increasing the copolymerization ratio m. Further, it can be said that the adjustment of the degree of polymerization n is most effective for adjusting the viscosity.
具体的に粘度、比重を制御した液体分離用ポリマーの
製造方法としては、ラジカル開始剤の存在下にスチレン
類とα，β−不飽和ジカルボン酸ジエステルとの共重合
反応を行なうに際して使用するα，β−不飽和ジカルボ
ン酸ジエステルの炭化水素基R3、R4の炭素数の選択や、
仕込みモル比の選択により共重合比ｍの調整が、また重
合温度の設定や重合溶剤の選択あるいはラジカル転移剤
の使用などにより重合度ｎの調整が可能で容易に特定の
粘度、比重を有する液体分離用ポリマーを製造すること
ができる。Specifically, as a method for producing a polymer for liquid separation in which viscosity and specific gravity are controlled, α, which is used when a copolymerization reaction of styrenes with α, β-unsaturated dicarboxylic acid diester in the presence of a radical initiator, is performed. Selection of the carbon number of the hydrocarbon group R 3 , R 4 of the β-unsaturated dicarboxylic acid diester,
It is possible to adjust the copolymerization ratio m by selecting the charged molar ratio, and the polymerization degree n by setting the polymerization temperature, selecting the polymerization solvent or using a radical transfer agent, etc. Separation polymers can be produced.
血清分離剤用のポリマーとしては、温度25℃における
比重が1.00〜1.08、好ましくは1.03〜1.06、また粘度が
1000〜150万cp、好ましくは１万〜60万cpの範囲が好適
に使用される。The polymer for the serum separating agent has a specific gravity at a temperature of 25 ° C of 1.00 to 1.08, preferably 1.03 to 1.06, and a viscosity of
A range of 1000 to 1.5 million cp, preferably 10,000 to 600,000 cp is preferably used.
この液体分離用ポリマーを液体分離剤として使用する
には、一般に知られている疎水性微粉末シリカや疎水性
スメクタイト粘土などのチクソトロピー化剤を添加分散
することにより、適度のチクソトロピー性を付与させ、
遠心力等の応力を加えることにより流動化を示し、通常
の状態では安定した非流動性を示すようなゲルを形成さ
せることによって使用される。To use the liquid separating polymer as a liquid separating agent, by adding and dispersing a generally known hydrophobic fine powder silica or a thixotropic agent such as hydrophobic smectite clay, a suitable thixotropic property is imparted,
It is used by forming a gel which exhibits fluidization by applying a stress such as centrifugal force and which exhibits stable non-fluidity under normal conditions.
次に本発明を実施例により更に具体的に説明するが、
本発明は、その要旨を超えない限り以下の実施例によっ
て限定されるものではない。Next, the present invention will be described more specifically with reference to Examples.
The present invention is not limited to the following examples unless it exceeds the gist.
（共重合体の製造例） 製造例１ １の４つ口フラスコに、マレイン酸ジブチルエステ
ル228g（１モル）を仕込み、窒素雰囲気下、160℃に加
熱し、次いでこれに撹拌下、スチレン104g（１モル）及
びジ−tert−ブチルペルオキシド8.76g（0.06モル）の
混合物を連続的に５時間かけて滴下し、添加後さらに同
温度で１時間、反応を行なわせた。反応終了後、１〜3m
mHgの減圧下に、反応混合物中に含まれる軽沸分および
未反応モノマーを留去して除き、スチレン−マレイン酸
ジブチルエステル共重合体309g（収率93％）を得た。得
られた共重合体は、無色透明な液体であり、25℃におけ
る粘度は30,000cp、比重は1.058であった。(Production Example of Copolymer) 228 g (1 mol) of maleic acid dibutyl ester was placed in a four-necked flask of Production Example 1 and heated to 160 ° C. under a nitrogen atmosphere, and then 104 g of styrene (under stirring). A mixture of 1 mol) and 8.76 g (0.06 mol) of di-tert-butyl peroxide was continuously added dropwise over 5 hours, and after the addition, the reaction was carried out at the same temperature for 1 hour. 1-3m after the reaction
The light-boiling components and unreacted monomers contained in the reaction mixture were distilled off under reduced pressure of mHg to obtain 309 g of styrene-maleic acid dibutyl ester copolymer (yield 93%). The obtained copolymer was a colorless transparent liquid, and had a viscosity at 25 ° C of 30,000 cp and a specific gravity of 1.058.
製造例２ マレイン酸ジブチルエステル１モルをフマル酸ジブチ
ルエステル160g（0.7モル）及びフマル酸ジ−２−エチ
ルヘキシルエステル102g（0.3モル）にかえたこと以外
は実施例１と同様にして、反応を行なわせて、スチレン
−フマル酸ジエステル共重合体329g（収率99％）を得
た。得られた共重合体は、無色透明な液体であり、25℃
における粘度は、90,000cp、比重は1.042であった。Production Example 2 The reaction was performed in the same manner as in Example 1 except that 1 mol of maleic acid dibutyl ester was replaced with 160 g (0.7 mol) of fumaric acid dibutyl ester and 102 g (0.3 mol) of fumaric acid di-2-ethylhexyl ester. Thus, 329 g of styrene-fumaric acid diester copolymer (yield 99%) was obtained. The obtained copolymer is a colorless transparent liquid at 25 ° C.
The viscosity was 90,000 cp and the specific gravity was 1.042.
製造例３〜５ 表１に示すα，β−不飽和ジカルボン酸ジエステルを
用い、表１に表わした反応条件とする他は、製造例１と
同様な方法で重合を行なった。得られた共重合体の性状
を製造例１〜２と併せて表１に示す。Production Examples 3 to 5 Polymerization was carried out in the same manner as in Production Example 1 except that the α, β-unsaturated dicarboxylic acid diester shown in Table 1 was used and the reaction conditions shown in Table 1 were used. The properties of the obtained copolymer are shown in Table 1 together with Production Examples 1 and 2.
（分離剤の製造） 実施例１ 製造例２で得られた共重合体100重量部とアエロジル
Ｒ−972（疎水性シリカ微粉末、日本アエロジル社製）
３重量部とを三本ロールにて十分に混練し、液体分離剤
を製造した。同様に表２に示す組成で液体分離剤を製造
した。これらの液体分離剤はいずれも適度なチクソトロ
ピー性を有し、遠心力等の応力を加えることにより流動
性を示し、通常の静置した状態ではゲルを形成した。 (Production of Separating Agent) Example 1 100 parts by weight of the copolymer obtained in Production Example 2 and Aerosil R-972 (hydrophobic silica fine powder, manufactured by Nippon Aerosil Co., Ltd.)
3 parts by weight was sufficiently kneaded with a triple roll to produce a liquid separating agent. Similarly, a liquid separating agent having the composition shown in Table 2 was produced. All of these liquid separating agents had an appropriate thixotropy property, exhibited fluidity by applying a stress such as centrifugal force, and formed a gel in a normal stationary state.
（評価−血清分離テスト） 実施例１で製造した各液体分離剤2ccと採血した全血
試料とを各スピッツ管に入れ、放置した。血液凝固が進
行し、血清と血餅とに分離したのち、3,000rpmで10分間
遠心分離したところ、いずれの液体分離剤についても、
血清と血餅の中間に液体分離剤のゲルが形成された。血
清はデカンテーションにより容易にスピッツ管から取り
出すことができた。 (Evaluation-Serum Separation Test) 2 cc of each liquid separating agent prepared in Example 1 and the collected whole blood sample were put into each Spitz tube and left to stand. After blood coagulation progressed and separated into serum and blood clot, centrifugation was performed at 3,000 rpm for 10 minutes.
A liquid separating agent gel was formed between the serum and the clot. Serum could be easily removed from the Spitz tube by decantation.
（評価−安定性） シリコーンオイル（比重d4 250.992、粘度100cp（25
℃））100重量部と疎水性シリカ微粉末（アエロジルＲ
−972、日本アエロジル社製）３重量部とを三本ロール
にて十分に混練し、チクソトロピー性のあるゲルを調製
した。(Evaluation-Stability) Silicone oil (specific gravity d 4 25 0.992, viscosity 100 cp (25
100 parts by weight and hydrophobic silica fine powder (Aerosil R)
-972, manufactured by Nippon Aerosil Co., Ltd.) and 3 parts by weight were sufficiently kneaded with a triple roll to prepare a gel having thixotropy.
（比較例） このゲルと、実施例１で製造した液体分離剤とを各2c
cずつ各スピッツ管に入れ、40℃で１週間保存してゲル
の安定性を見た。結果を表３に示す。(Comparative Example) This gel and the liquid separating agent prepared in Example 1 were each added to 2c.
Each c was placed in each Spitz tube and stored at 40 ° C for 1 week to check the stability of the gel. The results are shown in Table 3.
〔発明の効果〕 本発明の液体分離剤は、それに含まれる共重合体とチ
クソトロピー化剤との親和性が高く、また耐熱性が高い
ので、相分離による油分の分離成分への汚染や熱分解物
などによる分離成分への汚染などのトラブルを起こさ
ず、極めて実用上有用な特性を有している。 [Effect of the invention] The liquid separating agent of the present invention has a high affinity for the copolymer and the thixotropic agent contained therein, and also has a high heat resistance, so that the oil component due to phase separation is contaminated or pyrolyzed. It has extremely practically useful properties without causing troubles such as contamination of separated components due to things.
Claims (1)
ジカルボン酸ジエステルとの液状の共重合体を含有する
液体分離剤。1. The following general formula: A liquid separating agent containing a liquid copolymer of styrene and an α, β-unsaturated dicarboxylic acid diester having a structure represented by:
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61040898A JP2512426B2 (en) | 1986-02-26 | 1986-02-26 | Polymer for liquid separation |
| US07/013,340 US4828720A (en) | 1986-02-25 | 1987-02-11 | Liquid separating agent and liquid separating method |
| DE19873704854 DE3704854A1 (en) | 1986-02-25 | 1987-02-16 | LIQUID SEPARATORS AND METHOD FOR SEPARATING LIQUIDS |
| GB8703896A GB2188642B (en) | 1986-02-25 | 1987-02-19 | Agent for and method of separating components of a liquid |
| FR8702380A FR2598330B1 (en) | 1986-02-25 | 1987-02-24 | SEPARATING AGENT FOR CONSTITUENTS OF A LIQUID AND METHOD USING SUCH AN AGENT |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61040898A JP2512426B2 (en) | 1986-02-26 | 1986-02-26 | Polymer for liquid separation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62198759A JPS62198759A (en) | 1987-09-02 |
| JP2512426B2 true JP2512426B2 (en) | 1996-07-03 |
Family
ID=12593328
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61040898A Expired - Lifetime JP2512426B2 (en) | 1986-02-25 | 1986-02-26 | Polymer for liquid separation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2512426B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4504593B2 (en) * | 2001-06-05 | 2010-07-14 | 積水化学工業株式会社 | Serum or plasma separation composition |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58201814A (en) * | 1982-05-19 | 1983-11-24 | Nippon Oil & Fats Co Ltd | Synthesis of novel copolymer |
| JPS6128513A (en) * | 1984-07-19 | 1986-02-08 | Nippon Oil & Fats Co Ltd | Optical resin and its production |
| JPS6142320A (en) * | 1984-08-06 | 1986-02-28 | Nippon Oil & Fats Co Ltd | Oxygen permeable polymer molded body |
| JPS61211316A (en) * | 1985-03-16 | 1986-09-19 | Nippon Oil & Fats Co Ltd | Aromatic vinyl/fumaric diester copolymer |
-
1986
- 1986-02-26 JP JP61040898A patent/JP2512426B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62198759A (en) | 1987-09-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |