JP2506349B2 - Spreaded food - Google Patents
Spreaded foodInfo
- Publication number
- JP2506349B2 JP2506349B2 JP61256621A JP25662186A JP2506349B2 JP 2506349 B2 JP2506349 B2 JP 2506349B2 JP 61256621 A JP61256621 A JP 61256621A JP 25662186 A JP25662186 A JP 25662186A JP 2506349 B2 JP2506349 B2 JP 2506349B2
- Authority
- JP
- Japan
- Prior art keywords
- whey protein
- protease
- neutral
- food
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- Dairy Products (AREA)
Description
【発明の詳細な説明】 産業上の利用分野 本発明は、乳清より得られる乳清蛋白質を原料とする
スプレッド状(ゲル状)の物性をもった新規な食品に関
する。TECHNICAL FIELD OF THE INVENTION The present invention relates to a novel food product, which is made of whey protein obtained from whey and has a spread-like (gel-like) physical property.
従来の技術・発明が解決しようとする課題 乳清は乳からチーズを製造するさいに副生する黄色の
液体部分をさし、乳糖、乳清蛋白質、水溶性ビタミンお
よびミネラルを含んでいる。これらの乳清成分の中で栄
養的に最も価値が高いものは、乳清蛋白質である。すな
わち、各種の動物性および植物性蛋白質のうちで乳清蛋
白質は、アミノ酸バランス幼動物の成長促進効果か
らみて最も優れた蛋白質である。Wingord W.H.の報告に
よると体重70kgの成人男子の一日最低タン白必要量の比
較では、乳清蛋白質はわずか14.5gであるのに対し、代
表的な動物性蛋白の牛肉では19.3g、小麦蛋白では33.5g
を必要とする。食用としての乳清蛋白質は、飲料むけの
蛋白強化、肉製品の増量および組織改良、ドレッシング
用の乳化剤あるいは強い界面活性による気泡形成を利用
した菓子類、ケーキなどに用いられてきた。乳製品につ
いてはアイスクリームのコストダウン、チーズの組織改
良、育児用粉乳の調整など多くの用途が知られている。Problems to be Solved by the Related Art and Invention Whey refers to a yellow liquid part that is a by-product during the production of cheese from milk and contains lactose, whey protein, water-soluble vitamins and minerals. The most nutritionally valuable of these whey components is whey protein. That is, among various animal and plant proteins, whey protein is the most excellent protein in view of the growth promoting effect of amino acid-balanced larvae. According to a report by Wingord WH, whey protein is only 14.5 g in the daily minimum protein requirement of an adult male weighing 70 kg, whereas beef, a typical animal protein, has a protein content of 19.3 g and wheat protein. Then 33.5g
Need. Edible whey protein has been used for protein strengthening for beverages, increasing the amount of meat products and improving texture, dressing emulsifiers and confectioneries and cakes utilizing bubble formation due to strong surface activity. With regard to dairy products, many uses are known, such as cost reduction of ice cream, texture improvement of cheese, and preparation of powdered milk for childcare.
しかし乳清蛋白質を主成分とする食品は、一部のリコ
ッタ、ミゾストなどに限定されてきたのが現状である。However, at present, foods containing whey protein as a main component have been limited to some ricotta and mizost.
良質な蛋白源である畜肉および卵などは、程度の差こ
そあれすべてが動物性脂肪を含んだかたちで食用に供さ
れる。しかし、畜肉および卵に多量に含まれる飽和脂肪
酸およびコレステロールは、成人病を発症させる引きが
ねになることはよく知られている。従って、乳清蛋白の
ように脂肪を含まない良質蛋白は食料資源として貴重で
ある。All of the high-quality protein sources, such as meat and eggs, are served to the extent that they contain animal fat to varying degrees. However, it is well known that saturated fatty acids and cholesterol, which are contained in large amounts in meat and eggs, lead to the development of adult diseases. Therefore, high-quality protein containing no fat such as whey protein is valuable as a food resource.
さらに乳清蛋白は脂肪と高い親和性を持っているの
で、多量の脂肪を水中に乳化させることができる。この
ような物性は、水中に脂肪を乳化させた食品、例えば、
チーズ、チーズスプレッドなどの製造にあたっては貴重
な性質である。事実、多くのチーズスプレッドは蛋白質
のほかにスプレッド状の物性を持たせるため、バター、
クリームなどの脂肪、安定剤の添加をおこなっている。
しかも乳清蛋白をスプレッド状食品に利用するさいは、
動脈硬化の発症と相関を有する動物性脂肪の添加は避け
て、リノール酸、エイコサペンタエン酸などのような必
須脂肪酸を多量に含む油脂を任意に添加することができ
ることが大きな特徴である。Furthermore, whey protein has a high affinity for fats, so that a large amount of fats can be emulsified in water. Such physical properties are foods obtained by emulsifying fat in water, for example,
It is a valuable property in the production of cheese and cheese spread. In fact, many cheese spreads have butter, because they have spread-like properties in addition to protein.
Fats such as cream and stabilizers are added.
Moreover, when using whey protein for spread foods,
A major feature is that oils and fats containing a large amount of essential fatty acids such as linoleic acid and eicosapentaenoic acid can be added while avoiding the addition of animal fats that correlates with the onset of arteriosclerosis.
本発明者らは、乳清蛋白質を酵素によって部分分解す
ることによって製造したタン白質部分分解物をベースと
し、必要に応じて調味料、寒天、アルギン酸、グルコマ
ンナン、トラガカンドゴム、アラビアゴムなどの粘質多
糖、動物性ないし植物性油脂などを混合することによっ
て優れた風味を有する新規ゲル状食品の開発に成功し本
発明を完成した。The present inventors based on a protein partially decomposed product produced by partially decomposing whey protein with an enzyme, and if necessary, seasonings, agar, alginic acid, glucomannan, tragacanth gum, gum arabic, etc. The present invention has been completed by succeeding in developing a novel gel food having an excellent flavor by mixing a polysaccharide, an animal or vegetable oil and fat, and the like.
課題を解決するための手段 本発明において乳清蛋白質とは、チーズを製造する際
にに副生する新鮮乳清より低分子化合物を除き適当量ま
で濃縮したものかまたは市販の乳清蛋白濃縮粉末が用い
られる。この乳清蛋白の水溶液、好ましくは水分含量60
乃至90%、乳清蛋白含量10乃至20%のものを蛋白分解酵
素で部分分解することにより、スプレッド状の物性を有
する食品を製造することができる。Means for Solving the Problems In the present invention, whey protein is a whey protein concentrate powder or a commercially available whey protein concentrate that is concentrated to an appropriate amount excluding low molecular weight compounds from fresh whey that is a by-product when producing cheese. Is used. An aqueous solution of this whey protein, preferably with a water content of 60
It is possible to produce a food having spread-like physical properties by partially decomposing whey protein content of 90 to 90% and whey protein content of 10 to 20% with a protease.
本発明において用いる蛋白分解酵素は、プロチン、タ
カミネFPC、タシナーゼおよびパンチダーゼNP−2から
選択される。これらの蛋白分解酵素はそれぞれ以下の微
生物起源の酵素である。プロチンは、アスペルギルス属
(Aspergillus)起源の酸性プロテアーゼ、アスペルギ
ルス属起源の中性プロテアーゼ、枯草菌(Bacillus sub
tilis)起源の中性プロテアーゼまたは枯草菌起源のア
ルカリプロテアーゼであっていずれか1種または任意の
2種以上を混合して用いることができる。タカミネFPC
は、麹菌(Aspergillus oryzae)起源の酸性、中性およ
びアルカリ性プロテアーゼの混合物である。タシナーゼ
は放線菌(ストレプトミセス)起源の中性プロテアーゼ
である。パンチダーゼNP−2は、麹菌(Aspergillus or
yzae)起源のプロテアーゼである。次表にこれらの酵素
を使用し乳清蛋白水溶液を部分分解した結果を示す。The proteolytic enzyme used in the present invention is selected from protin, Takamine FPC, tacinase and punchidase NP-2. These proteolytic enzymes are the following microbial origin enzymes, respectively. Protin is an acidic protease derived from the genus Aspergillus, a neutral protease derived from the genus Aspergillus, and Bacillus subtilis.
tilis) -derived neutral protease or Bacillus subtilis-derived alkaline protease, and any one kind or a mixture of two or more kinds can be used. Takamine FPC
Is a mixture of acidic, neutral and alkaline proteases of Aspergillus oryzae origin. Tacinase is a neutral protease of Streptomyces origin. Punchidase NP-2 is an Aspergillus or
yzae) is a protease of origin. The following table shows the results of partial decomposition of an aqueous whey protein solution using these enzymes.
反応条件45℃、添加量3,000pu/g。発酵処理還元乳清
蛋白濃縮物(全固形物28.1%、脂肪1.35%、蛋白21.4%
を使用した。なお、表中「パンチダーゼ」はパンチダー
ゼNP−2を意味する。 Reaction conditions 45 ° C, addition amount 3,000 pu / g. Fermentation reduced whey protein concentrate (total solids 28.1%, fat 1.35%, protein 21.4%
It was used. In the table, "punchidase" means punchdase NP-2.
表から明らかなようにいずれの蛋白分解酵素を使用し
ても、作用時間が30分では苦味が感じられる。90分処理
ではチーズフレーバー様な風味が生じた。また、使用し
た酵素ではタシナーゼのみがチーズとは異質のレバー様
の臭みがあるフレーバーとなった。この場合のレバー臭
とは風味効果が劣っていることを示すものではなく、こ
のレバー様の臭みは、例えば、フォアグラ様の食品等に
利用することができるものである。そのほかの酵素を分
解したさいは大差がない結果が得られた。As is clear from the table, no matter which proteolytic enzyme is used, bitterness is felt when the action time is 30 minutes. A 90-minute treatment produced a cheese flavor-like flavor. Moreover, among the enzymes used, tacinase alone had a liver-like flavor that was different from cheese. The liver odor in this case does not indicate that the flavor effect is inferior, and this lever-like odor can be utilized for, for example, foie gras-like food. When the other enzymes were decomposed, the result was almost the same.
次に酵素の最適添加量を求めるために、添加量を段階
的に変化させて検討した。具体的には、例えば酵素とし
てプロチンを用い、乳酸発酵を平行させるため乳酸菌の
スターターを同時に添加し、反応条件は温度32℃、6時
間、基質は還元乳清蛋白濃縮物(pH6.1)を用いて行っ
た結果、酵素添加量0.01乃至0.02%ではほとんど苦味が
なくうまみを感じ、酵素を0.04%に増量すると苦味を感
ずるようになることが判明した。酵素の最適添加量は約
0.01乃至0.02%程度と考えられる。Next, in order to obtain the optimum addition amount of the enzyme, the addition amount was changed stepwise and examined. Specifically, for example, using protin as an enzyme, a lactic acid bacterium starter was added at the same time to parallel lactic acid fermentation, the reaction conditions were a temperature of 32 ° C. for 6 hours, and the substrate was a reduced whey protein concentrate (pH 6.1). As a result, it was found that when the amount of enzyme added was 0.01 to 0.02%, almost no bitterness was felt and umami was felt, and when the amount of enzyme was increased to 0.04%, bitterness was felt. The optimum amount of enzyme added is approximately
It is considered to be about 0.01 to 0.02%.
本発明のスプレッド状食品には、調味料、アルギン
酸、ブルコマンナン、トラガンドゴム、アラビアゴムな
どの粘質多糖類および動物性ないし植物性油脂等を適宜
加えることにより一層優れた風味を有するものとするこ
とができる。また風味を改良するために蛋白分解酵素に
よる部分分解と同時に乳酸発酵処理することも可能であ
る。The spread-like food of the present invention is to have a more excellent flavor by appropriately adding a seasoning, alginic acid, bruccomannan, gum tragacanth, viscous polysaccharides such as gum arabic and animal or vegetable fats and oils. You can Further, in order to improve the flavor, it is also possible to carry out the lactic acid fermentation treatment simultaneously with the partial decomposition by the protease.
実施例1 ベースミックスとしては粉末乳清蛋白20%(W/V,以下
同様)、植物油脂15%、乳糖2.0%、食塩2.0%を水に懸
濁し、混合溶解させた。次に温度を60℃まで上昇させて
から均質機にかけ150kg/cm2でホモジナイズした。この
間液の温度は63〜68℃に維持して、予備的に殺菌する。Example 1 As a base mix, 20% powdered whey protein (W / V, the same applies hereinafter), 15% vegetable oil and fat, 2.0% lactose, and 2.0% salt were suspended in water and mixed and dissolved. Next, the temperature was raised to 60 ° C., and the mixture was homogenized with a homogenizer at 150 kg / cm 2 . During this period, the temperature of the liquid is maintained at 63 to 68 ° C to sterilize it preliminarily.
実施例2 実施例1によって作成したベースミックス9部に対
し、粉末乳清蛋白質20%を水に懸濁し、63℃に30分間保
持して殺菌したのち、45℃に冷却した。45℃に保持した
混合液にプロチンFNの液1.2%を添加して90分間、加水
分解を行った。90分後にキサンタンガム、グアガム、ゼ
ラチンを各々0.1%ずつ添加し、80℃、10分間攪拌しつ
つ加熱してチーズ状スプレッド食品を得た。Example 2 To 9 parts of the base mix prepared in Example 1, 20% of powdered whey protein was suspended in water, kept at 63 ° C for 30 minutes for sterilization, and then cooled to 45 ° C. 1.2% of Protin FN solution was added to the mixed solution kept at 45 ° C., and hydrolysis was performed for 90 minutes. After 90 minutes, 0.1% of xanthan gum, 0.1% of guar gum, and 0.1% of gelatin were added, respectively, and heated at 80 ° C. for 10 minutes with stirring to obtain a cheese-like spread food.
実施例3 実施例1によって製造したベースミックスを40℃まで
冷却し、40℃に保持しつつ乳酸菌スターター、0.5%お
よびプロチンFNを0.02%添加し、6時間処理した。この
処理によって液のpHは4.5〜5.0になる。その時点で実施
例2に示す安定剤を0.5%添加し、80℃に10分間加熱し
てチーズ状スプレッド食品を得た。Example 3 The base mix produced according to Example 1 was cooled to 40 ° C., and while being kept at 40 ° C., a lactic acid bacterium starter, 0.5% and protin FN 0.02% were added and treated for 6 hours. This treatment brings the pH of the liquid to 4.5 to 5.0. At that time, 0.5% of the stabilizer shown in Example 2 was added and heated at 80 ° C. for 10 minutes to obtain a cheese-like spread food product.
実施例4. チーズから回収した乳清を限外濾過によって35倍に濃
縮してから得られた乳清蛋白質濃縮物(蛋白として50
%)、68部に生クリーム30部および食塩2部を混合し、
63℃に30分間保持して殺菌したのち、40℃に冷却した。
この混合物に乳酸菌スターター5.0%およびプロチンFN
0.02%を添加し、液のpHが4.5〜5.0に低下するまで5〜
6時間、40℃で保持した。次いで80℃で10分間攪拌しつ
つ加熱してチーズ状スプレッド食品を得た。Example 4. Whey protein concentrate obtained by concentrating whey recovered from cheese 35 times by ultrafiltration (50% as protein)
%), 68 parts mixed with 30 parts fresh cream and 2 parts salt,
After holding at 63 ° C for 30 minutes for sterilization, it was cooled to 40 ° C.
Lactic acid bacteria starter 5.0% and Protin FN in this mixture
Add 0.02%, until the pH of the solution drops to 4.5-5.0
Hold at 40 ° C for 6 hours. Then, the mixture was heated at 80 ° C. for 10 minutes with stirring to obtain a cheese-like spread food product.
Claims (3)
ペルギルス属(Aspergillus)起源の酸性プロテアー
ゼ、アスペルギルス属起源の中性プロテアーゼ、枯草菌
(Bacillus subtilis)起源の中性プロテアーゼおよび
枯草菌起源のアルカリプロテアーゼ、(2)麹菌(Aspe
rgillus oryzae)起源の酸性、中性およびアルカリ性プ
ロテアーゼの混合物、(3)放線菌(ストレプトミセ
ス)起源の中性プロテアーゼ並びに(4)麹菌(Asperg
illus oryzae)起源のプロテアーゼからなる群より選択
される蛋白分解酵素によって処理して得られるスプレッ
ド状食品。1. A liquid containing whey protein as a main component (1) an acidic protease of Aspergillus origin, a neutral protease of Aspergillus origin, a neutral protease of Bacillus subtilis and Bacillus subtilis Original alkaline protease, (2) Aspergillus (Aspe
rgillus oryzae) -derived mixture of acidic, neutral and alkaline proteases, (3) Neutral proteases of Streptomyces and (4) Asperg
illus oryzae) -derived foodstuff processed by a proteolytic enzyme selected from the group consisting of proteases.
%を主成分とする液を用いる特許請求の範囲第1項記載
の食品。2. Whey protein 10 to 20 with water content 60 to 90%
The food product according to claim 1, wherein a liquid containing 100% as a main component is used.
処理することによって得られる特許請求の範囲第1項ま
たは第2項記載の食品。3. A proteolytic enzyme is added in an amount of about 0.01 to 0.02%,
The food according to claim 1 or 2, which is obtained by processing.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61256621A JP2506349B2 (en) | 1986-10-28 | 1986-10-28 | Spreaded food |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61256621A JP2506349B2 (en) | 1986-10-28 | 1986-10-28 | Spreaded food |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS63109737A JPS63109737A (en) | 1988-05-14 |
JP2506349B2 true JP2506349B2 (en) | 1996-06-12 |
Family
ID=17295158
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP61256621A Expired - Lifetime JP2506349B2 (en) | 1986-10-28 | 1986-10-28 | Spreaded food |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2506349B2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2663014B2 (en) * | 1989-05-22 | 1997-10-15 | 雪印乳業株式会社 | Cheese curd containing whey protein and method for producing cheese using the same as raw material |
JP2683492B2 (en) * | 1993-09-07 | 1997-11-26 | 雪印乳業株式会社 | Micellar whey protein, solution thereof, powder thereof, and method for producing micellar whey protein |
JP2607344B2 (en) * | 1993-09-30 | 1997-05-07 | 雪印乳業株式会社 | Method for producing gelled whey protein and method for producing processed food using whey protein |
US20060057131A1 (en) * | 2001-12-20 | 2006-03-16 | Eric Simard | Malleable protein matrix and uses thereof |
DK2409575T3 (en) | 2008-10-17 | 2017-04-03 | Nestec Sa | Process for Preparation of Whey Protein Compositions |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5347560A (en) * | 1976-10-13 | 1978-04-28 | Pfizer | Functioned protein and method of producing same |
-
1986
- 1986-10-28 JP JP61256621A patent/JP2506349B2/en not_active Expired - Lifetime
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5347560A (en) * | 1976-10-13 | 1978-04-28 | Pfizer | Functioned protein and method of producing same |
Also Published As
Publication number | Publication date |
---|---|
JPS63109737A (en) | 1988-05-14 |
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