JP2024517104A - Ｃａｒｅエレメントを用いたｄｎａ増幅方法 - Google Patents

Ｃａｒｅエレメントを用いたｄｎａ増幅方法 Download PDF

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Publication number
JP2024517104A
JP2024517104A JP2023564038A JP2023564038A JP2024517104A JP 2024517104 A JP2024517104 A JP 2024517104A JP 2023564038 A JP2023564038 A JP 2023564038A JP 2023564038 A JP2023564038 A JP 2023564038A JP 2024517104 A JP2024517104 A JP 2024517104A
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Japan
Prior art keywords
aav
nucleic acid
gene
nucleotide sequence
host cell
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Pending
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JP2023564038A
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English (en)
Japanese (ja)
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JP2024517104A5 (https=
JPWO2022223954A5 (https=
Inventor
ケイウッド、ライアン
ブライティ、デイビッド
ギルマン、アダム
スー、ウェイヘン
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Oxford Genetics Ltd
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Oxford Genetics Ltd
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Publication date
Priority claimed from GBGB2105581.9A external-priority patent/GB202105581D0/en
Priority claimed from GBGB2110922.8A external-priority patent/GB202110922D0/en
Priority claimed from GBGB2113603.1A external-priority patent/GB202113603D0/en
Priority claimed from GBGB2116571.7A external-priority patent/GB202116571D0/en
Application filed by Oxford Genetics Ltd filed Critical Oxford Genetics Ltd
Publication of JP2024517104A publication Critical patent/JP2024517104A/ja
Publication of JP2024517104A5 publication Critical patent/JP2024517104A5/ja
Publication of JPWO2022223954A5 publication Critical patent/JPWO2022223954A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023564038A 2021-04-19 2022-04-14 Ｃａｒｅエレメントを用いたｄｎａ増幅方法 Pending JP2024517104A (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GB2105581.9 2021-04-19
GBGB2105581.9A GB202105581D0 (en) 2021-04-19 2021-04-19 DNA amplification method
GB2110922.8 2021-07-29
GBGB2110922.8A GB202110922D0 (en) 2021-07-29 2021-07-29 DNA amplification method
GBGB2113603.1A GB202113603D0 (en) 2021-09-23 2021-09-23 DNA amplification method
GB2113603.1 2021-09-23
GB2116571.7 2021-11-17
GBGB2116571.7A GB202116571D0 (en) 2021-11-17 2021-11-17 Dna amplification method
PCT/GB2022/050957 WO2022223954A1 (en) 2021-04-19 2022-04-14 Dna amplification method using care elements

Publications (3)

Publication Number Publication Date
JP2024517104A true JP2024517104A (ja) 2024-04-19
JP2024517104A5 JP2024517104A5 (https=) 2025-04-21
JPWO2022223954A5 JPWO2022223954A5 (https=) 2025-04-21

Family

ID=81386652

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023564038A Pending JP2024517104A (ja) 2021-04-19 2022-04-14 Ｃａｒｅエレメントを用いたｄｎａ増幅方法

Country Status (4)

Country Link
US (1) US20250066811A1 (https=)
EP (1) EP4326881A1 (https=)
JP (1) JP2024517104A (https=)
WO (1) WO2022223954A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201901571D0 (en) 2019-02-05 2019-03-27 Oxford Genetics Ltd Inducible AAV sysyem
AU2022373653A1 (en) 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides
CN119842820A (zh) * 2023-10-16 2025-04-18 深圳市深研生物科技有限公司 用于生产重组腺相关病毒载体的方法及包装/生产细胞
WO2026073032A1 (en) * 2024-09-27 2026-04-02 Shape Therapeutics Inc. Constructs for improved aav production

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100008979A1 (en) * 2004-06-10 2010-01-14 Saint Louis University Delivery of therapeutic agents to the bone

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002240890A1 (en) 2000-12-07 2002-06-18 Universite De Nantes Inducible highly productive raav packaging cell-lines
US9523132B2 (en) * 2013-03-15 2016-12-20 Ut-Battelle, Llc Rapid and highly fieldable viral diagnostic
CN110892064A (zh) 2017-07-25 2020-03-17 牛津遗传学有限公司 腺病毒载体

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100008979A1 (en) * 2004-06-10 2010-01-14 Saint Louis University Delivery of therapeutic agents to the bone

Also Published As

Publication number Publication date
EP4326881A1 (en) 2024-02-28
WO2022223954A1 (en) 2022-10-27
US20250066811A1 (en) 2025-02-27

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