JP2024041706A - Method of predicting oxytocin levels of infants - Google Patents
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Abstract
Description
本発明は、乳児の定常状態のオキシトシンレベルを予測する方法に関する。 The present invention relates to a method for predicting steady state oxytocin levels in infants.
オキシトシンは養育者と子どもとの間の愛着形成に関与する、脳由来のペプチドホルモンである（非特許文献１）。オキシトシンは乳幼児の社会性の発達向上に関与するため（非特許文献２、非特許文献３）、乳幼児期にオキシトシンを上昇させることは、社会性向上に伴う、より良い家族・社会の形成に繋がり、養育者の育児ストレス軽減にも寄与すると考えられる。 Oxytocin is a brain-derived peptide hormone that is involved in the formation of attachment between caregivers and children (Non-Patent Document 1). Oxytocin is involved in improving the social development of infants (Non-Patent Document 2, Non-Patent Document 3), so increasing oxytocin in early childhood will lead to the formation of better families and society as a result of improved sociability. It is thought that this also contributes to reducing childcare stress for caregivers.
これまで成人女性において、手の平（掌）で布生地に触れた後の触感評価値と、触れた前後のオキシトシン変化率が相関を示すことが明らかになっている(特許文献１)。また、オキシトシンの分泌にはオートクラインシステム(自己分泌調節機構)が存在し、数週間、継続してオキシトシンが上昇する刺激が行われると、定常状態(ベースライン)のオキシトシンレベルが上昇することが知られている(特許文献２、非特許文献４)。 It has been revealed that in adult women, the tactile evaluation value after touching a cloth with the palm of the hand correlates with the rate of change in oxytocin before and after touching (Patent Document 1). In addition, an autocrine system (self-crine regulation mechanism) exists for the secretion of oxytocin, and if a stimulus that increases oxytocin is applied continuously for several weeks, the steady state (baseline) oxytocin level will increase. known (Patent Document 2, Non-Patent Document 4).
乳児は、おむつを始め、衣類、タオル、寝具類など、一定の繊維性物品に継続的に接触して生活しており、当該繊維性物品による継続的な接触刺激が定常状態のオキシトシンレベルに影響を及ぼす可能性が高いと考えられる。したがって、当該乳児の定常状態のオキシトシンレベルを把握することは、乳児がおかれている生活環境が適切か否かを客観的に判断することに繋がり有用である。 Infants live in continuous contact with certain fibrous items such as diapers, clothing, towels, and bedding, and the continuous contact stimulation from these fibrous items affects steady-state oxytocin levels. It is considered that there is a high possibility that Therefore, understanding the steady-state oxytocin level of the infant is useful for objectively determining whether or not the infant's living environment is appropriate.
しかしながら、乳児におけるオキシトシンの測定は一般的に唾液や尿で行われることから、必要なタイミングでサンプルを採取することは難しい。そのため、簡便に乳児のオキシトシンレベルを把握する方法が望まれている。 However, since oxytocin in infants is generally measured in saliva or urine, it is difficult to collect samples at the required timing. Therefore, there is a need for a method for easily determining oxytocin levels in infants.
本発明は、乳児のオキシトシンレベルを予測する方法を提供することに関する。 The present invention relates to providing a method for predicting oxytocin levels in infants.
本発明者らは、乳幼児が日常で使用している日用品(紙おむつ、自宅の洗剤、柔軟剤で洗濯し、乾燥した後の繊維性物品（衣類とタオル）に母親が触れた触感と、それに触れて生活している乳幼児のオキシトシンの関連性を解析したところ、母親の当該物品に対する触感評価値(なめらかさ、やわらかさ)及び母親による乳児の肌状態の評価値が当該乳児の唾液中オキシトシン量にのみ有意に正相関し、斯かる触感評価値又は肌状態評価値、更にはこれらを組み合わせて利用することにより乳児のオキシトシンレベルが予測可能であることを見出した。斯かる結果は、養育者と子どものオキシトシンの相関性については相反する報告（Feldman et al., Horm. Behav., 58, 669-676, 2010、Fujiwara et al., Psychoneuroendocrinology., 102, 172-181, 2019)があることを考えると、意外なことである。 The present inventors investigated the tactile sensation of a mother's touch on daily necessities that infants and young children use on a daily basis (disposable diapers, fibrous articles (clothing and towels) that have been washed and dried with household detergent and fabric softener, and When we analyzed the relationship between oxytocin in infants living in the home, we found that the mother's tactile evaluation of the object (smoothness, softness) and the mother's evaluation of the infant's skin condition were associated with the amount of oxytocin in the infant's saliva. They found that the oxytocin level of infants can be predicted by using the tactile evaluation value or the skin condition evaluation value, or even a combination of these. There are conflicting reports regarding the correlation of oxytocin in children (Feldman et al., Horm. Behav., 58, 669-676, 2010, Fujiwara et al., Psychoneuroendocrinology., 102, 172-181, 2019). When you think about it, this is surprising.
すなわち、本発明は、以下の１）～５）に係るものである。
１）乳児のオキシトシンレベルを予測する方法であって、以下の工程（Ａ）～（Ｃ）を含む方法：
（Ａ）所定の繊維性物品に触れて生活している乳児の母親の皮膚に、前記繊維性物品を接触させる工程、
（Ｂ）前記繊維性物品との接触による触感を評価する工程、
（Ｃ）前記触感評価値から、前記乳児のオキシトシン量の予測値を算出する工程。
２）乳児のオキシトシンレベルを予測する方法であって、以下の工程（Ｄ）～（Ｅ）を含む方法：
（Ｄ）乳児の母親が、当該乳児の肌状態を評価する工程、
（Ｅ）前記肌状態評価値から、前記乳児のオキシトシン量の予測値を算出する工程。
３）１）の方法において得られる触感評価値と、２）の方法において得られる肌状態評価値を用いて乳児のオキシトシン量の予測値を算出する、乳児のオキシトシンレベルを予測する方法。
４）１）の方法により予測されたオキシトシンレベルを用いる、当該乳児が使用する繊維性物品、又は当該繊維性物品の加工又は処理手段の分類又は評価方法。
５）２）の方法により予測されたオキシトシンレベルを用いる、乳児が使用するスキンケア品の分類又は評価方法。
That is, the present invention relates to the following 1) to 5).
1) A method for predicting an infant's oxytocin level, comprising the steps of:
(A) contacting a specified textile article with the skin of a mother of an infant who lives in contact with the textile article;
(B) evaluating the tactile sensation upon contact with the fibrous article;
(C) A process of calculating a predicted value of the infant's oxytocin amount from the tactile evaluation value.
2) A method for predicting an infant's oxytocin level, comprising the steps of:
(D) a step in which the mother of the infant evaluates the skin condition of the infant;
(E) A step of calculating a predicted value of the infant's oxytocin amount from the skin condition evaluation value.
3) A method for predicting an infant's oxytocin level, comprising calculating a predicted value of the infant's oxytocin amount using the tactile evaluation value obtained by the method in 1) and the skin condition evaluation value obtained by the method in 2).
4) A method for classifying or evaluating a textile article used by the infant, or a processing or treatment method for the textile article, using the oxytocin level predicted by the method of 1).
5) A method for classifying or evaluating skin care products for use by infants, using the oxytocin level predicted by the method in 2).
本発明の方法によれば、乳児の定常状態のオキシトシンレベルを、測定の手間をかけずに簡易に予測できる。斯かるオキシトシンレベルは乳児が日常使用する繊維製物品、又は当該繊維性物品の加工又は処理手段を分類又は評価するため、或いは乳児が日常使用するスキンケア品を分類又は評価するための指標となり得る。 According to the method of the present invention, an infant's steady-state oxytocin level can be easily predicted without the hassle of measurement. Such oxytocin levels can be an indicator for classifying or evaluating textile articles used daily by infants, or processing or treatment means for such textile articles, or skin care products used daily by infants.
後述する実施例に示すように、３－１１ヶ月齢児（乳児）が常時使用する紙おむつ、自宅の洗剤及び柔軟剤処理した繊維性物品（衣類とタオル）に対する母親の触感評価値(なめらかさ、やわらかさ)と当該乳児の唾液中オキシトシン量には有意な正相関が認められた。
また、乳児の母親によって評価された当該乳児の肌状態の評価値と当該乳児の唾液中オキシトシン量には有意に正相関が認められた。
これらの結果は、所定の繊維性物品に触れて生活している乳児の母親の皮膚に当該繊維性物品を接触させた場合の触感評価値から、当該乳児のオキシトシンレベルが予測可能であることを示し、また、乳児の母親による当該乳児の肌状態評価値から、当該乳児のオキシトシンレベルが予測可能であることを示す。
As shown in the examples below, mothers' tactile evaluation values (smoothness, A significant positive correlation was observed between the amount of oxytocin in the infant's saliva (softness) and the amount of oxytocin in the infant's saliva.
Furthermore, a significant positive correlation was observed between the evaluation value of the infant's skin condition evaluated by the infant's mother and the amount of oxytocin in the infant's saliva.
These results demonstrate that the oxytocin level of an infant can be predicted from the tactile evaluation value when the fibrous article is brought into contact with the skin of the mother of the infant who lives in contact with the fibrous article. It also shows that the oxytocin level of an infant can be predicted from the skin condition evaluation value of the infant by the infant's mother.
本発明の乳児のオキシトシンレベルを予測する方法の一態様は、以下の工程（Ａ）～（Ｃ）により実行される。
（Ａ）所定の繊維性物品に触れて生活している乳児の母親の皮膚に、前記繊維性物品を接触させる工程
（Ｂ）前記繊維性物品との接触による触感を評価する工程
（Ｃ）前記触感評価値から、前記乳児のオキシトシン量の予測値を算出する工程
One embodiment of the method for predicting oxytocin levels in infants of the present invention is carried out by the following steps (A) to (C).
(A) A step of bringing the fibrous article into contact with the skin of the mother of an infant who lives in contact with a predetermined fibrous article.(B) A step of evaluating the tactile sensation caused by contact with the fibrous article.(C) A step of the above. Calculating a predicted value of the amount of oxytocin in the infant from the tactile evaluation value
本発明において、オキシトシンレベルの予測対象である乳児は３－１１ヶ月齢児である。
乳児は所定の繊維性物品に触れて生活しているが、工程（Ａ）における、所定の繊維性物品とは、乳児が日常生活において継続して肌に触れることが多い繊維性物品であり、例えば、紙おむつ、タオル、肌着等の衣類、寝具のシーツ、毛布、掛け布団カバー、枕ケース、タオルケット等が挙げられ、好ましくは紙おむつ、タオル、肌着である。なお、斯かる繊維性物品には、洗剤又は洗剤及び柔軟剤のような繊維処理剤で処理された繊維性物品、例えば洗濯・乾燥後の繊維性物品が包含される。
In the present invention, infants whose oxytocin levels are to be predicted are 3-11 months old.
Infants live their lives by touching predetermined fibrous articles, and in step (A), the predetermined fibrous articles are fibrous articles that infants often come into contact with on a continuous basis in their daily lives; Examples include clothing such as paper diapers, towels, and underwear, bedding sheets, blankets, duvet covers, pillow cases, towel blankets, etc., and paper diapers, towels, and underwear are preferred. Note that such fibrous articles include fibrous articles treated with a detergent or a fiber treatment agent such as a detergent and a fabric softener, for example, fibrous articles after washing and drying.
オキシトシンは、９個のアミノ酸残基からなるペプチドホルモンであり、大脳の視床下部の室傍核や視索上核に存在する大細胞性神経細胞で合成され、脳下垂体後葉から血中に放出されることが知られている。
オキシトシンの分泌にはオートクラインシステム(自己分泌調節機構)が存在し、数週間、継続してオキシトシンが上昇する刺激が行われると、定常状態(ベースライン)のオキシトシンレベルが上昇することが知られている(前記特許文献２、非特許文献４)。
本発明において予測されるオキシトシンレベルは、所定の繊維性物品に触れて日常生活を送っている乳児において認められる定常状態のオキシトシンレベルである。
ここで、オキシトシンレベルとは、放出されたオキシトシン量を意味するが、具体的には血中オキシトシン量、唾液中オキシトシン量、尿中オキシトシン量が挙げられ、好ましくは唾液中オキシトシン量である。
Oxytocin is a peptide hormone consisting of nine amino acid residues, synthesized by magnocellular neurons located in the paraventricular nucleus and supraoptic nucleus of the hypothalamus of the cerebrum, and released into the blood from the posterior pituitary gland. It is known that
It is known that an autocrine system (self-crine regulation mechanism) exists in the secretion of oxytocin, and that when a stimulus that increases oxytocin is applied continuously for several weeks, the steady-state (baseline) oxytocin level increases. (Patent Document 2, Non-Patent Document 4).
The oxytocin levels predicted in the present invention are the steady state oxytocin levels observed in infants who interact with a given fibrous article and go about their daily lives.
Here, the oxytocin level refers to the amount of oxytocin released, and specifically includes the amount of oxytocin in blood, the amount of oxytocin in saliva, and the amount of oxytocin in urine, and preferably the amount of oxytocin in saliva.
工程（Ａ）において、母親の皮膚と繊維性物品の接触は、母親自身が自発的に行ってもよく、強制的に行ってもよいが、自発的に行うのが好ましい。接触部としては、皮膚であればよく、例えば、顔、手指や掌、手の甲等の手、上腕、肘、下腕、脚、足裏等が挙げられるが、無毛部（手指、掌、足裏）が好ましく、より好ましくは手指や掌である。
好ましい接触の態様としては、母親自身が手の指先と掌で当該物品に触れる態様が挙げられ、具体的には手の指先と掌を前後左右に動かして当該物品に触れる態様が挙げられる。
In step (A), the mother's skin may be brought into contact with the fibrous article either voluntarily or by force, but it is preferably done voluntarily. The contact area may be skin, such as the face, fingers, palms, hands such as the back of the hands, upper arms, elbows, lower arms, legs, soles of the feet, etc. The back) is preferred, and the fingers and palm are more preferred.
A preferred mode of contact includes a mode in which the mother herself touches the article with her fingertips and a palm of her hand, and specifically a mode in which she touches the article by moving her fingertips and palm back and forth and from side to side.
繊維性物品への接触時間及び接触回数等の接触態様は適宜設定することができ、連続的又は断続的に行ってもよいが、一定時間試験物品に接触させた後に接触を解除して安静状態とし、再び接触させる動作を何回か繰り返して行う態様が挙げられる。例えば、試験物品に３０秒間触れた後に、試験物品から手を離し、何も触れずに３０秒間安静にする動作を数回（例えば、３～５回）繰り返すことが挙げられる。 The contact mode such as the contact time and the number of times of contact with the fibrous article can be set as appropriate, and it may be done continuously or intermittently, but after contacting the test article for a certain period of time, the contact is released and the test article is in a resting state. An example of this is a mode in which the operation of making contact again is repeated several times. For example, after touching the test article for 30 seconds, removing the hand from the test article and resting for 30 seconds without touching anything may be repeated several times (for example, 3 to 5 times).
工程（Ｂ）における、繊維性物品との接触による触感評価は、当該繊維性物品が持つなめらかさ、やわらかさ、あたたかさ、ふんわり感等の、幸福・満足感、リラックス、リフレッシュ等の感情を喚起させる触感（風合い）が評価されるが、このうち、なめらかさ又はやわらかさを評価するのが好ましく、なめらかさ及びやわらかさを併せて評価するのがより好ましい。 The tactile evaluation by contact with the fibrous article in step (B) is based on the smoothness, softness, warmth, fluffiness, etc. of the fibrous article that evokes feelings of happiness, satisfaction, relaxation, refreshment, etc. The tactile feel (texture) of the product is evaluated, and among these, it is preferable to evaluate smoothness or softness, and it is more preferable to evaluate smoothness and softness together.
触感評価の手法は、例えば、当該繊維性物品に触れた時の触感（例えば、なめらかさ、やわらかさ、あたたかさ）を１０ｃｍのＶｉｓｕａｌ Ａｎａｌｏｇ Ｓｃａｌｅ（ＶＡＳ）を用いて、感覚量の程度を示す方法が挙げられる。
ＶＡＳ法では、直線の左端を「例えば、なめらかでない、やわらかくない、又はあたたかくない」、右端を「例えば、非常になめらか、非常にやわらかい、又は非常にあたたかい」とし、被験者である母親が該当する箇所に線を引き、左端から線を引いた箇所までの距離（ｃｍ）を各触感評価値とされる。
The tactile evaluation method is, for example, a method of expressing the tactile sensation (e.g., smoothness, softness, warmth) when touching the fibrous article using a 10 cm Visual Analog Scale (VAS). can be mentioned.
In the VAS method, the left end of the straight line is defined as "for example, not smooth, soft, or warm," and the right end is defined as "for example, very smooth, very soft, or very warm." A line is drawn on the screen, and the distance (cm) from the left end to the point where the line is drawn is taken as each tactile evaluation value.
次いで、前記触感評価値から、前記乳児のオキシトシン量の予測値が算出される（工程（Ｃ））。
後述する実施例に示すように、日常で使用中の繊維性物品の母親の触感評価値（なめらかさ評価値、やわらかさ評価値）と当該乳児の唾液中オキシトシン量は、有意な正相関を示す。
したがって、母親の前記繊維性物品との接触により得られた触感評価値を、乳児のオキシトシン量予測式に代入することにより、乳児のオキシトシン量の予測値を算出することができる。予測式は、１つの触感評価値（例えば、なめらかさ評価値又はやわらかさ評価値）を使用する場合、当該触感評価値とオキシトシン量との散布図（図１、３）から単回帰分析を利用することにより、以下のように作成することができる。
Next, a predicted value of the amount of oxytocin in the infant is calculated from the tactile evaluation value (step (C)).
As shown in the examples described below, there is a significant positive correlation between the mother's tactile evaluation value (smoothness evaluation value, softness evaluation value) of fibrous articles used on a daily basis and the amount of oxytocin in the infant's saliva. .
Therefore, by substituting the tactile evaluation value obtained by the mother's contact with the fibrous article into the formula for predicting the amount of oxytocin in the infant, it is possible to calculate the predicted value of the amount of oxytocin in the infant. When using one tactile evaluation value (e.g., smoothness evaluation value or softness evaluation value), the prediction formula uses a simple regression analysis from the scatter diagram of the tactile evaluation value and the amount of oxytocin (Figures 1 and 3). By doing so, it can be created as follows.
１）紙おむつのなめらかさ評価値による予測式
（数１）
ｙ＝４．５３ｘ＋１４．７８
（ｙ：オキシトシン量、ｘ：紙おむつのなめらかさ評価値、ｒ＝０．４７、ｐ＜０．０５）
２）紙おむつのやわらかさ評価値による予測式
（数２）
ｙ＝４．９５ｘ＋１０．１３
（ｙ：オキシトシン量、ｘ：紙おむつのやわらかさ評価値、ｒ＝０．４７、ｐ＜０．１） ３）洗剤及び柔軟剤処理繊維性物品（衣類とタオル）のなめらかさ評価値による予測式（数３）
ｙ＝８．２３ｘ－０．４０
（ｙ：オキシトシン量、ｘ：洗剤及び柔軟剤処理繊維性物品（衣類とタオル）のなめらかさ評価値、ｒ＝０．６０、ｐ＜０．０５）
４）洗剤及び柔軟剤処理繊維性物品（衣類とタオル）のやわらかさ評価値による予測式（数４）
ｙ＝６．６２ｘ＋５．６３
（ｙ：オキシトシン量、ｘ：洗剤及び柔軟剤処理繊維性物品（衣類とタオル）のやわらかさ評価値、ｒ＝０．４９、ｐ＜０．０５）
1) Prediction formula based on the smoothness evaluation value of disposable diapers (Equation 1)
y=4.53x+14.78
(y: oxytocin amount, x: smoothness evaluation value of disposable diaper, r=0.47, p<0.05)
2) Prediction formula based on the softness evaluation value of disposable diapers (Equation 2)
y=4.95x+10.13
(y: oxytocin amount, x: softness evaluation value of disposable diapers, r = 0.47, p < 0.1) 3) Prediction formula based on smoothness evaluation values of detergent and softener treated fibrous articles (clothing and towels) (Number 3)
y=8.23x-0.40
(y: amount of oxytocin, x: evaluation value of smoothness of fibrous articles (clothing and towels) treated with detergent and fabric softener, r=0.60, p<0.05)
4) Prediction formula (Equation 4) based on the softness evaluation value of detergent and softener treated fibrous articles (clothing and towels)
y=6.62x+5.63
(y: oxytocin amount, x: softness evaluation value of detergent and fabric softener treated fibrous articles (clothing and towels), r=0.49, p<0.05)
また、複数の触感評価値（例えば、なめらかさ評価値及びやわらかさ評価値）を使用して乳児のオキシトシン量予測式を作成するには重回帰分析を利用することができる。一例として、２つの触感評価値と乳児のオキシトシン量との散布図（図１、３）から、以下のような予測式を導くことができる。 Moreover, multiple regression analysis can be used to create a formula for predicting the amount of oxytocin in an infant using a plurality of tactile evaluation values (for example, a smoothness evaluation value and a softness evaluation value). As an example, the following prediction formula can be derived from a scatter diagram (FIGS. 1 and 3) between two tactile evaluation values and the infant's oxytocin amount.
洗剤及び柔軟剤処理繊維性物品（衣類とタオル）のなめらかさ、やわらかさ評価値による予測式
（数５）
ｙ＝７．５６ｘ１＋０．８７ｘ２－１．８２
（ｙ：オキシトシン量、ｘ１：洗剤及び柔軟剤処理繊維性物品（衣類とタオル）のなめらかさ評価値、ｘ２：洗剤及び柔軟剤処理繊維性物品（衣類とタオル）のやわらかさ評価値、自由度調整済み決定係数：０．２８、ｐ＜０．０５）
Prediction formula (Equation 5) based on smoothness and softness evaluation values of detergent and softener treated fibrous articles (clothing and towels)
y=7.56x1+0.87x2-1.82
(y: Oxytocin amount, x1: Smoothness evaluation value of detergent and fabric softener treated fibrous articles (clothing and towels), x2: Softness evaluation value of detergent and fabric softener treated fibrous articles (clothing and towels), degree of freedom Adjusted coefficient of determination: 0.28, p<0.05)
本発明の乳児のオキシトシンレベルを予測する方法の別の一態様は、以下の工程（Ｄ）～（Ｅ）により実行される。
（Ｄ）工程において、乳児の母親による当該乳児の肌状態の評価としては、例えば、“これまで「皮膚が弱い」、「敏感」、「あれやすい」、「肌トラブルが起きやすい」と感じたことがあるか”という設問に対して、５件法（１：あてはまる、２：ややあてはまる、３：どちらともいえない、４：ややあてはまらない、５：あてはまらない）で評価することが挙げられる。
Another embodiment of the method of predicting oxytocin levels in infants of the present invention is carried out by the following steps (D) to (E).
In the (D) process, the infant's mother's evaluation of the infant's skin condition includes, for example, ``I have always felt that my skin is weak,''``sensitive,'' ``prone to irritation,'' and ``prone to skin problems.'' In response to the question "Do you have any experience with this?", you can evaluate it using a 5-point scale (1: Yes, 2: Somewhat applies, 3: Can't say either way, 4: Somewhat doesn't apply, 5: Doesn't apply).
前記肌状態評価値から、前記乳児のオキシトシン量の予測値の算出（工程Ｅ）は、母親による乳児の肌状態評価スコアと、その乳児の唾液中オキシトシン量とが有意な正相関することから、前述の工程（Ｃ）と同様に、乳児の母親による当該乳児の肌状態評価値を、乳児のオキシトシン量予測式に代入することにより、乳児のオキシトシン量の予測値を算出することができる。
予測式は、肌状態評価スコアとオキシトシン量との散布図（図５（Ａ））から単回帰分析を利用することにより、以下のように作成することができる。
（数６）
ｙ＝１１．４３ｘ＋４．４３
（ｙ：オキシトシン量、ｘ：乳児肌状態評価値、ｒ＝０．５２、ｐ＜０．０５）
Calculation of the predicted value of the amount of oxytocin in the infant from the skin condition evaluation value (step E) is performed because there is a significant positive correlation between the skin condition evaluation score of the infant by the mother and the amount of oxytocin in the infant's saliva. Similarly to the above step (C), by substituting the infant's skin condition evaluation value by the infant's mother into the formula for predicting the amount of oxytocin in the infant, the predicted value of the amount of oxytocin in the infant can be calculated.
The prediction formula can be created as follows by using a simple regression analysis from a scatter diagram of the skin condition evaluation score and the amount of oxytocin (FIG. 5(A)).
(Number 6)
y=11.43x+4.43
(y: oxytocin amount, x: infant skin condition evaluation value, r=0.52, p<0.05)
また、上記の工程（Ｂ）により得られる触感評価値と、工程（Ｄ）により得られる肌状態評価値を用いて乳児のオキシトシン量の予測値を算出することにより、オキシトシンレベルの予測精度の向上を図ることができる。例えば、後述する実施例に示すように、母親による紙おむつのなめらかさ評価値と母親による乳児の肌状態評価値を用いることによって、紙おむつのなめらかさ評価値のみによる予測に比べて高い精度で乳児の唾液中オキシトシン量を予測することが可能となる。 Furthermore, by calculating the predicted value of the infant's oxytocin amount using the tactile evaluation value obtained in step (B) and the skin condition evaluation value obtained in step (D), the prediction accuracy of the oxytocin level is improved. can be achieved. For example, as shown in the example below, by using the mother's evaluation value of the smoothness of the disposable diaper and the mother's evaluation value of the infant's skin condition, the baby's skin condition can be predicted with higher accuracy than the prediction based only on the diaper smoothness evaluation value. It becomes possible to predict the amount of oxytocin in saliva.
斯くして、予測された乳児のオキシトシンレベルによれば、乳児がおかれている生活環境が適切か否かを客観的に把握することができる。すなわち、工程（Ａ）～（Ｃ）によって予測された乳児のオキシトシンレベルは、例えば、乳児が日常使用する繊維性物品、特に紙おむつや、洗濯・乾燥後の繊維性物品、具体的には洗剤又は洗剤及び柔軟剤処理した身の周りの繊維性物品(衣類・タオル)が当該乳児に適しているか又は適するか否かの分類或いは評価の他、乳児に適した繊維性物品の加工又は処理手段、例えば乳児に適した繊維の織り方、乳児に適した繊維処理剤を乳児の視点から分類或いは評価するために使用することができる。また、工程（Ｄ）～（Ｅ）によって予測された乳児のオキシトシンレベルは、例えば、乳児に使用するスキンケア品が当該乳児に適しているか又は適するか否かの分類或いは評価に使用することができる。ここで、乳児に使用するスキンケア品とは、所謂乳児用スキンケア化粧品が挙げられ、マイルドで低刺激性、保湿に優れるという特徴や機能を持つ肌洗浄剤、ローション、乳液、クリーム、化粧水、ジェル等が挙げられる。
したがって、本発明の方法は、乳児のオキシトシンレベルを的確に向上させることに貢献し、ひいては子どもの社会性・協調性の発達向上に寄与する環境づくりに貢献できる。
According to the predicted oxytocin level of the infant, it is possible to objectively determine whether the living environment in which the infant is placed is appropriate. That is, the infant's oxytocin level predicted by steps (A) to (C) is, for example, fibrous articles that infants use on a daily basis, especially disposable diapers, fibrous articles after washing and drying, specifically detergents, In addition to classifying or evaluating whether household fibrous articles (clothing/towels) treated with detergents and softeners are suitable for infants, processing or treatment methods for fibrous articles suitable for infants; For example, it can be used to classify or evaluate fiber weaving methods suitable for infants and fiber treatment agents suitable for infants from the infant's perspective. In addition, the infant's oxytocin level predicted by steps (D) to (E) can be used, for example, to classify or evaluate whether a skin care product for use on an infant is suitable or not suitable for the infant. . Here, skin care products used for infants include so-called skin care cosmetics for infants, including skin cleansers, lotions, milky lotions, creams, lotions, and gels that have the characteristics and functions of being mild, hypoallergenic, and highly moisturizing. etc.
Therefore, the method of the present invention can contribute to accurately increasing oxytocin levels in infants and, in turn, contribute to creating an environment that contributes to improving the development of social and cooperative skills in children.
以下、実施例に基づき本発明をさらに詳細に説明するが、本発明はこれに限定されるものではない。
試験例１ 所定の繊維性物品に対する母親の触感評価値を用いた乳児の唾液中オキシトシンレベルの予測
１. 方法
（１）試験参加者
３－２４ヶ月齢の乳幼児と、その母親６４組を解析の対象者とした。
Hereinafter, the present invention will be explained in more detail based on Examples, but the present invention is not limited thereto.
Test Example 1 Prediction of salivary oxytocin levels in infants using mothers' tactile evaluation values for specified fibrous articles 1. Method (1) Test Participants 64 pairs of infants aged 3 to 24 months and their mothers were analyzed. The target audience was
（２）母親の日用品の触感評価値
乳幼児と、その母親が日常で使用している紙おむつ、または自宅の洗剤及び柔軟剤で洗濯し、乾燥した後の繊維性物品(衣類とタオル)に掌で触れた時の触感（なめらかさ、やわらかさ、あたたかさ）を１０ｃｍのＶｉｓｕａｌ Ａｎａｌｏｇ Ｓｃａｌｅ（ＶＡＳ；一定の長さの直線上に感覚量の程度を示す方法。直線の左端を「なめらかでない、やわらかくない、または、あたたかくない」、右端を「非常になめらか、非常にやわらかい、または、非常にあたたかい」とし、該当する箇所に線を引く）で、各母親が評価した。左端から線を引いた箇所までの距離（ｃｍ）を各触感評価値とした。なお日常で使用している日用品の触感評価を行うため、日常使用の紙おむつ、洗剤／柔軟剤の銘柄・ブランドは測定１ヶ月前から変更禁止とした。
(2) Tactile evaluation value of mothers' daily necessities Infants and their mothers touch paper diapers that they use on a daily basis, or fibrous items (clothing and towels) that have been washed with household detergent and fabric softener and dried. The tactile sensation (smoothness, softness, warmth) when touched is measured using a 10cm Visual Analog Scale (VAS; a method of indicating the degree of sensation on a straight line of a certain length. Each mother evaluated the texture using the following criteria: ``Very smooth, very soft, or not very warm,'' and ``Very smooth, very soft, or very warm,'' on the far right, and draw a line under the appropriate area. The distance (cm) from the left end to the point where the line was drawn was defined as each tactile evaluation value. In order to perform a tactile evaluation of everyday items used on a daily basis, the brands and brands of disposable diapers and detergents/softeners used on a daily basis were not allowed to be changed for one month prior to the measurement.
（３）乳幼児からの唾液採取
１５－２０時の間に、１回／日、３日間連続で乳幼児から唾液を採取した。Ｓａｌｉｍｅｔｒｉｃｓ Ｉｎｆａｎｔ’ｓ Ｓｗａｂを乳幼児に約１－２分間咥えさせることを複数回繰り返して（最大１０分間）、Ｓｗａｂに唾液を染み込ませた。各Ｓｗａｂから唾液を回収して、複数日で採取した唾液は、等量ずつ混合してオキシトシン測定用のサンプルとし、－８０℃で保存した。なお唾液採取の１時間前から、授乳、水（湯）以外の飲食、歯磨きを禁止とした。
(3) Saliva collection from infants Saliva was collected from infants once a day for three consecutive days between 15:00 and 20:00. The Salimetrics Infant's Swab was held in the infant's mouth for approximately 1-2 minutes multiple times (up to 10 minutes) to saturate the Swab with saliva. Saliva was collected from each Swab, and the saliva collected over multiple days was mixed in equal amounts to form a sample for oxytocin measurement, and stored at -80°C. For 1 hour before saliva collection, the subjects were prohibited from breastfeeding, eating or drinking anything other than water (hot water), and brushing their teeth.
（４）唾液中オキシトシン測定
回収した唾液（０．１－１．０ｍｌ）と等量の０．１％（ｖ／ｖ）トリフルオロ酢酸（ＴＦＡ）を混和し、遠心分離（３，０００ｒｐｍ、３０ｍｉｎ）した後の上清を、Ｓｅｐ－ｐａｋ Ｃ１８カラム（２００ｍｇ、３ｃｃ、Ｗａｔｅｒｓ）に供し、抽出を行った。 まず、Ｃ１８カラムに１．０ｍｌの１００％アセトニトリル（ＡＣＮ）、次いで１０ｍｌの０．１％ＴＦＡ溶液（ｖ／ｖ）を通し、その後で０．１％ＴＦＡ溶液（ｖ／ｖ）と混和した唾液サンプル（１．０－６．０ｍｌ）を通し、１０ｍｌの０．１％ＴＦＡ溶液（ｖ／ｖ）で洗浄した後、３．０ｍｌの（９５％ＡＣＮ／５％（０．１％ＴＦＡ溶液））（ｖ／ｖ）で溶出させた。溶出した溶液のＡＣＮを窒素ガスで揮発させ、残った溶液を凍結乾燥に供し、凍結乾燥品をＯｘｙｔｏｃｉｎ ＥＬＩＳＡ ｋｉｔ（Ｅｎｚｏ）中のＡｓｓａｙ Ｂｕｆｆｅｒの２５０μｌで溶解して、前記キットを用いて定量した。Ｂｉｏ－ｒ
ａｄ Ｐｒｏｔｅｉｎ ａｓｓａｙ（ＢＩＯ－ＲＡＤ）で、ウシ血清アルブミンで作成した検量線を基に、抽出前の唾液中のタンパク質濃度（ｍｇ／ｍｌ）を定量し、単位タンパク量当たりのオキシトシン量を算出した。
(4) Measurement of oxytocin in saliva The collected saliva (0.1-1.0 ml) was mixed with an equal amount of 0.1% (v/v) trifluoroacetic acid (TFA), and centrifuged (3,000 rpm, 30 min). ), the supernatant was applied to a Sep-pak C18 column (200 mg, 3 cc, Waters) for extraction. First, 1.0 ml of 100% acetonitrile (ACN), then 10 ml of 0.1% TFA solution (v/v) was passed through the C18 column, and then saliva mixed with 0.1% TFA solution (v/v) was passed through the C18 column. The sample (1.0-6.0 ml) was passed through and washed with 10 ml of 0.1% TFA solution (v/v), followed by 3.0 ml of (95% ACN/5% (0.1% TFA solution) ) (v/v). ACN in the eluted solution was volatilized with nitrogen gas, the remaining solution was subjected to lyophilization, the lyophilized product was dissolved in 250 μl of Assay Buffer in the Oxytocin ELISA kit (Enzo), and quantified using the kit. Bio-r
Using ad protein assay (BIO-RAD), the protein concentration (mg/ml) in saliva before extraction was determined based on a calibration curve prepared with bovine serum albumin, and the amount of oxytocin per unit amount of protein was calculated.
２．結果
（１）紙おむつ、洗剤及び柔軟剤処理繊維性物品(衣類とタオル)の触感評価値と乳幼児のオキシトシンとの関連性解析
日常で使用中の紙おむつの母親の触感評価値と乳幼児の唾液中オキシトシン量との関連性解析を行った。その結果、乳児の母親のなめらかさ、及びやわらかさ評価値と、その乳児の唾液中オキシトシン量とが有意な正相関を示した（図１；なめらかさ：ｒ＝０．６０，ｐ＜０．０５、やわらかさ：ｒ＝０．５１，ｐ＜０．０５、あたたかさ：ｒ＝０．３４，ｐ＝０．１６、Spearmanの順位相関係数）。一方で、幼児の母親の紙おむつの触感評価値と、その幼児の唾液中オキシトシン量とでは有意な相関は認められなかった（図２；なめらかさ：ｒ＝０．１８，ｐ＝０．２４、やわらかさ：ｒ＝０．０２，ｐ＝０．８７、あたたかさ：ｒ＝０．０７，ｐ＝０．６６、Spearmanの順位相関係数）。
2. Results (1) Analysis of the relationship between the tactile evaluation values of disposable diapers, detergent- and softener-treated fibrous articles (clothing and towels) and oxytocin in infants Mother's tactile evaluation value of disposable diapers in daily use and salivary oxytocin of infants We conducted an analysis of the relationship with the amount. As a result, there was a significant positive correlation between the smoothness and softness evaluation values of the infant's mother and the amount of oxytocin in the infant's saliva (Figure 1; smoothness: r=0.60, p<0. 05, Softness: r=0.51, p<0.05, Warmth: r=0.34, p=0.16, Spearman's rank correlation coefficient). On the other hand, there was no significant correlation between the infant's mother's tactile evaluation of the disposable diaper and the amount of oxytocin in the infant's saliva (Figure 2; smoothness: r = 0.18, p = 0.24, Softness: r=0.02, p=0.87, Warmth: r=0.07, p=0.66, Spearman's rank correlation coefficient).
（２）また、日常で使用中の洗剤及び柔軟剤で処理した繊維性物品(衣類とタオル)の母親の触感評価値と乳幼児の唾液中のオキシトシン量との関連性解析を行った結果、乳児の母親のなめらかさ、及びやわらかさ評価値と、その乳児の唾液中オキシトシン量とが有意な正相関を示した（図３；なめらかさ：ｒ＝０．６２，ｐ＜０．０５、やわらかさ：ｒ＝０．５１，ｐ＜０．０５、あたたかさ：ｒ＝０．４４，ｐ＝０．０７、Spearmanの順位相関係数）。一方で、幼児の母親の洗剤及び柔軟剤処理繊維性物品(衣類とタオル)の触感評価値と、その幼児の唾液中オキシトシン量とでは、有意な相関は認められなかった（図４；なめらかさ：ｒ＝０．０６，ｐ＝０．６７、やわらかさ：ｒ＝０．０４，ｐ＝０．７６、あたたかさ：ｒ＝０．０８，ｐ＝０．６１、Spearmanの順位相関係数）。
（３）これらのことから日常で使用中の紙おむつ、及び洗剤及び柔軟剤処理繊維性物品(
衣類とタオル)の、乳児の母親による、なめらかさ・やわらかさ評価値で、その乳児のオ
キシトシンが評価可能であることが示された。
(2) In addition, as a result of an analysis of the relationship between mothers' evaluation of the tactile sensation of fibrous articles (clothing and towels) treated with detergents and fabric softeners in everyday use and the amount of oxytocin in infants' saliva, There was a significant positive correlation between mothers' smoothness and softness evaluation values and their infants' salivary oxytocin levels (Figure 3; smoothness: r = 0.62, p < 0.05, softness : r=0.51, p<0.05, warmth: r=0.44, p=0.07, Spearman's rank correlation coefficient). On the other hand, there was no significant correlation between the infant's mother's tactile evaluation of detergent- and fabric softener-treated fibrous articles (clothing and towels) and the amount of oxytocin in the infant's saliva (Figure 4; : r=0.06, p=0.67, softness: r=0.04, p=0.76, warmth: r=0.08, p=0.61, Spearman's rank correlation coefficient) .
(3) For these reasons, disposable diapers and detergent- and softener-treated fibrous articles (
It has been shown that oxytocin in infants can be evaluated based on the infant's mother's evaluation of the smoothness and softness of clothing (clothing and towels).
試験例２ 母親による乳児の肌状態価値を用いた乳児の唾液中オキシトシンレベルの予測
１. 方法
（１）試験参加者
３－２４ヶ月齢の乳幼児と、その母親６４組を解析の対象者とした。
Test Example 2 Prediction of oxytocin levels in infants' saliva using mothers' scores on infants' skin condition 1. Method (1) Test participants Sixty-four pairs of infants aged 3-24 months and their mothers were subjects of the analysis.
（２）母親による乳幼児の肌状態評価
乳幼児の肌状態を、その母親が評価した。“これまで「皮膚が弱い」、「敏感」、「あれやすい」、「肌トラブルが起きやすい」と感じたことがあるか”という設問に対して、５件法で評価した（１：あてはまる、２：ややあてはまる、３：どちらともいえない、４：ややあてはまらない、５：あてはまらない）。
(2) Mother's evaluation of infant's skin condition The infant's skin condition was evaluated by the mother. In response to the question, “Have you ever felt that your skin is weak, sensitive, prone to irritation, or prone to skin problems?”, a 5-item scale was used to evaluate the response (1: Yes, 2: Somewhat applicable, 3: Can't say either way, 4: Somewhat not applicable, 5: Not applicable).
（３）乳幼児からの唾液採取
試験例１（３）と同様に、乳幼児から唾液を採取した。
(3) Saliva collection from infants Saliva was collected from infants in the same manner as Test Example 1 (3).
（４）唾液中オキシトシン測定
試験例１（４）と同様に、採取した唾液中のオキシトシン量を測定した。
(4) Measurement of oxytocin in saliva The amount of oxytocin in the collected saliva was measured in the same manner as in Test Example 1 (4).
２．結果
母親が評価した乳幼児の肌状態評価スコアと、その乳幼児の唾液中オキシトシン量との関連性解析を行った結果、母親による乳児の肌状態評価スコアと、その乳児の唾液中オキシトシン量とが有意な正相関を示した（図５（Ａ）；ｒ＝０．５５，ｐ＜０．０５）。また単回帰分析を実施した結果、有意差が認められた（ｙ＝１１．４３ｘ＋４．４３，ｒ＝０．５２，ｐ＜０．０５，ｙ：乳児唾液中オキシトシン量，ｘ：乳児肌状態評価スコア）。
一方で、母親が評価した幼児の肌状態評価スコアと、その幼児の唾液中オキシトシン量とでは有意な相関は認められず（図５（Ｂ）；ｒ＝－０．０３，ｐ＝０．８３）、単回帰分析でも有意差は認められなかった（ｙ＝－１．１６ｘ＋４１．５３，ｒ＝－０．０６，ｐ＝０．６９，ｙ：幼児唾液中オキシトシン量，ｘ：幼児肌状態評価スコア）。
2. Results An analysis of the relationship between the infant's skin condition evaluation score evaluated by the mother and the amount of oxytocin in the infant's saliva revealed that there was a significant correlation between the infant's skin condition evaluation score by the mother and the amount of oxytocin in the infant's saliva. A positive correlation was shown (FIG. 5(A); r=0.55, p<0.05). In addition, as a result of simple regression analysis, a significant difference was observed (y = 11.43x + 4.43, r = 0.52, p < 0.05, y: amount of oxytocin in infant saliva, x: infant skin condition evaluation Score).
On the other hand, there was no significant correlation between the skin condition evaluation score of the infant evaluated by the mother and the amount of oxytocin in the infant's saliva (Figure 5 (B); r = -0.03, p = 0.83 ), no significant difference was observed in simple regression analysis (y = -1.16x + 41.53, r = -0.06, p = 0.69, y: oxytocin amount in infant saliva, x: infant skin condition evaluation Score).
試験例３ 乳児の母親の紙おむつのなめらかさ評価値、及び乳児の肌状態評価スコアによる、乳児の唾液中オキシトシン量の予測
乳児の母親による紙おむつのなめらかさ評価値と、乳児の肌状態評価スコアを説明変数に、乳児の唾液中オキシトシンレベルを目的変数に設定して重回帰分析を実施した結果、有意差が認められ（ｙ＝４．１２ｘ１＋１０．５１ｘ２＋１０．５１，ｒ＝０．６３，ｒ２＝０．３９，ｐ＜０．０５，ｙ：乳児唾液中オキシトシン量，ｘ１：紙おむつのなめらかさ評価値，ｘ２：肌状態評価スコア）、紙おむつのなめらかさ評価値のみによる予測（ｙ＝４．５３ｘ＋１４．７８，ｒ＝０．４７，ｒ２＝０．２２，ｐ＜０．０５，ｙ：乳児唾液中オキシトシン量，ｘ：紙おむつのなめらかさ評価値）より、決定係数が高い値を示した。
このことから、紙おむつのなめらかさ評価値のみより、紙おむつのなめらかさ評価値と乳児の肌状態評価スコアで乳児の唾液中オキシトシン量を予測する方が高い精度を示すことが明らかになった。
Test Example 3 Prediction of the amount of oxytocin in an infant's saliva based on the infant's mother's evaluation of the smoothness of a disposable diaper and the infant's skin condition evaluation score. As a result of conducting multiple regression analysis with the infant's salivary oxytocin level set as the explanatory variable and objective variable, a significant difference was observed (y = 4.12x 1 + 10.51x 2 + 10.51, r = 0.63 , r2=0.39, p<0.05, y: amount of oxytocin in infant saliva, x 1 : smoothness evaluation value of the disposable diaper, x 2 : skin condition evaluation score), prediction based only on the smoothness evaluation value of the disposable diaper ( y = 4.53x + 14.78, r = 0.47, r2 = 0.22, p < 0.05, y: amount of oxytocin in infant saliva, x: evaluation value of smoothness of disposable diaper). showed that.
From this, it became clear that predicting the amount of oxytocin in an infant's saliva using the diaper's smoothness evaluation value and the infant's skin condition evaluation score was more accurate than using the diaper's smoothness evaluation value alone.
Claims (8)
（Ａ）所定の繊維性物品に触れて生活している乳児の母親の皮膚に、前記繊維性物品を接触させる工程、
（Ｂ）前記繊維性物品との接触による触感を評価する工程、
（Ｃ）前記触感評価値から、前記乳児のオキシトシンレベルの予測値を算出する工程。 A method of predicting oxytocin levels in an infant, the method comprising the following steps (A) to (C):
(A) a step of bringing the fibrous article into contact with the skin of the mother of an infant who lives in contact with a predetermined fibrous article;
(B) a step of evaluating the tactile sensation upon contact with the fibrous article;
(C) calculating a predicted value of the infant's oxytocin level from the tactile evaluation value;
（Ｄ）乳児の母親が、当該乳児の肌状態を評価する工程、
（Ｅ）前記肌状態評価値から、前記乳児のオキシトシン量の予測値を算出する工程。 A method for predicting oxytocin levels in infants, the method comprising the following steps (D) to (E):
(D) a step in which the infant's mother evaluates the infant's skin condition;
(E) Calculating a predicted value of the amount of oxytocin in the infant from the skin condition evaluation value.
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