JP2024020094A - Use of low-dose glutathione as a skin brightening agent - Google Patents

Abstract

An object of the present invention is to improve skin brightness using glutathione. SOLUTION: The present inventors have discovered a method of improving skin brightness even with low doses of glutathione, and have completed the present invention. [Selection diagram] None

Description

The present invention relates to a glutathione-containing composition suitably used for whitening and beautifying the skin and improving skin brightness, and a method for whitening and beautifying the skin and improving skin brightness.

Although the mechanisms behind the development of skin pigmentation, age spots, and freckles are still unclear, one reason is that melanin pigments are formed due to hormonal abnormalities and stimulation from ultraviolet rays, and this is deposited within the skin. It is considered. Tyrosinase is an enzyme that catalyzes the oxidation of tyrosine or dopa, etc., and inhibiting tyrosinase activity suppresses the production of melanin pigment. It is used as an anti-browning agent.

Glutathione is a tripeptide consisting of amino acids such as cysteine, glutamic acid, and glycine, and is known as a drug that improves liver detoxification. Ingestion of glutathione is known to improve non-alcoholic fatty liver disease, and also functions as a kokumi substance, and is used in food materials, medicines, etc.

In addition, when glutathione is orally ingested, the intake is generally 100 mg/day to 300 mg/day, and Non-Patent Document 1 states that by taking 250 mg or more, the amount of glutathione in the blood increases after 6 months. It is reported that the number has increased by 17%. In Non-Patent Document 2, 500 mg/day was taken for 4 weeks and the effect on skin melanin was observed.

Furthermore, it has been reported that glutathione can be used in combination with other materials to improve skin conditions. Patent Document 1 reports that α-lipoic acid and reduced glutathione inhibit tyrosinase activity.

JP2009-51768

Richie JP Jr, Nichenametla S, Neidig W, et al. Randomized controlled trial of oral glutathione supplementation on body stores of glutathione. Eur J Nutr. 2015;54(2):251-263. Arjinpathana, Nutthavuth, Glutathione as an oral whitening agent: A randomized, double-blind, placebo-controlled study. Journal of Dermatological Treatment 2012;23(2): 97-102

An object of the present invention is to improve skin brightness using glutathione.

The present inventors have discovered a method for improving skin brightness even at low doses of glutathione, and have completed the present invention.

The present invention is as follows.
(1) Oral agent for improving skin brightness containing glutathione as an active ingredient;
(2) The oral preparation for improving skin brightness according to (1) above, which has a glutathione content of 100 mg or less;
(3) the oral preparation for improving skin brightness according to (1) or (2) above, wherein the glutathione is glutathione contained in yeast extract;
(4) A method for improving skin brightness by orally ingesting 100 mg/day or less of glutathione;
(5) The method for improving skin brightness according to (4) above, wherein the glutathione is glutathione contained in yeast extract.

According to the present invention, skin brightness can be improved even if the intake of glutathione is 100 mg/day or less.

Arm brightness measurement results

Improving skin brightness in the present invention refers to increasing the brightness of the skin, and generally refers to bright skin, whitening effect, prevention of melanin pigmentation, improvement of melanin pigmentation, etc.

Glutathione as used in the present invention refers to reduced glutathione, oxidized glutathione, or a mixture thereof. Reduced glutathione is a tripeptide with the structure γ-L-Glu-L-Cys-Gly, and oxidized glutathione is two molecules of reduced glutathione bound together through an S-S bond. It is. The form of glutathione may be any form as long as it contains glutathione as an active ingredient.

The glutathione of the present invention is not particularly limited as long as it can be obtained by a method that can be used as a medicine or food. Usually, methods are obtained from microorganisms such as yeast (JP 59-151894), methods produced by synthesis using enzymes (JP 2020-72), and methods produced by chemical synthesis (Bull. Chem., Soc. Jpn., 1980, Vol. 53, p. 2529), and there are no limitations on the glutathione used in the present invention. Moreover, glutathione and oxidized glutathione can be purchased, and any purchased products may be used as long as they can be used as foods, medicines, etc.

The glutathione content in the composition of the present invention is arbitrary, but since the daily intake is 100 mg or less, the content in the composition is preferably 100 mg or less. Furthermore, when the composition of the present invention is in the form of a tablet, for example, the content per tablet may be 100 mg, or, for example, the content per tablet may be 20 mg, and the composition of the present invention may be used for 5 tablets. . The same applies to granules, liquids, etc.

The glutathione used in the glutathione-containing composition of the present invention is not particularly limited as long as it is in a form containing glutathione, such as a purified product, a crudely purified product, or a mixture. The mixture may be an extract containing glutathione, such as yeast extract. Examples of yeast containing glutathione include "Haithion Cobo MG" (Mitsubishi Corporation Life Sciences), and yeast extracts containing glutathione include "Haithion Extract YH" (Mitsubishi Corporation Life Sciences).

In the present invention, the glutathione content in a mixture such as yeast extract is measured using high performance liquid chromatography (HPLC). The measurement conditions are as follows.
・Column: Mighty Sil RP-18GP (Kanto Chemical) 4.6mm-150cm
・Mobile phase: 5% diammonium hydrogen phosphate (adjusted to pH 3.2 with phosphoric acid)
・Measurement wavelength: 210nm

Furthermore, the total amount of glutathione including GSH and GSSG is determined according to the method of Tietze ("Analytical Biochemistry" Vol. 27, p. 502, 1969). Note that the glutathione content in the present invention refers to the total glutathione content including reduced and oxidized forms.

In the present invention, glutathione has an effect even if it is a purified product if taken in low doses, but it is even better if it is a glutathione-containing yeast extract. As the yeast extract, it is particularly preferable to use a yeast extract with a high content of glutathione. Particularly preferred is a yeast extract containing 8% by weight or more of glutathione, and more preferably a yeast extract containing 10% by weight or more. Particularly preferred is an enzyme extract containing 15% by weight or more.

Other substances may be added to the composition of the present invention as long as the effects of the present invention are not impaired. Things that can be added include regular supplements, feed, excipients added to medicines, various vitamins, inorganic substances such as calcium, magnesium, iron, and zinc, organic acids such as lactic acid, citric acid, and malic acid, proteins, and lactic acid bacteria. Organic substances such as extracts, polyphenols, and plant extracts may be added as appropriate. In addition, acidulants, sweeteners, flavoring agents, stabilizers, and the like that are added to general foods can also be added.

The method of administering the product of the present invention is not particularly limited, and can include oral administration, parenteral administration such as intravenous, intraperitoneal, or subcutaneous administration. Specifically, oral preparations such as tablets, powders, granules, pills, suspensions, emulsions, infusions/decoctions, capsules, syrups, solutions, elixirs, extracts, tinctures, and liquid extracts; Or parenteral preparations such as injections, drops, creams, and suppositories may be used. Conventional methods can be used to prepare these tablets, etc., and there are no limitations in the present invention.

The dosage of the present invention is effective at low doses. When administered to humans, the intake amount and frequency of administration vary depending on the dosage form, age and weight of the recipient, but adults should ingest 100 mg or less of glutathione per day. Usually, the amount is taken once to several times a day in an amount of 10 mg to 100 mg, preferably 20 mg to 80 mg, particularly preferably 30 mg to 50 mg. When using yeast extract containing glutathione, measure the glutathione content in the yeast extract and adjust the intake amount to meet the dosage in this paragraph.

The composition of the present invention can be ingested not only as a medicine but also as a functional food or a nutritional supplement, and in that case, the composition should be ingested to the same amount as the previous intake amount. The composition of the present invention can be used as a composition for improving, preventing, and treating lifestyle-related diseases such as metabolic syndrome.

The invention of the present application will be explained in more detail below. However, the present invention is not limited by the following explanation.

(Test method)
In a randomized, placebo-controlled, double-blind, parallel-group comparative intervention study, healthy adults aged 20 to 65 years were asked to consume test foods for 12 weeks, and the effect on skin brightness was examined.

(Test food)
- Yeast extract A is a yeast extract containing 15% by weight of glutathione, and the glutathione content in test food A is 80 mg of glutathione.

- The amount of yeast extract A in test food B is half the amount of test food A, and the glutathione content in test food B is 40 mg of glutathione.
- Yeast extract B used in the control food is a yeast extract that does not contain glutathione, so the glutathione content in the control food is 0 mg.
(Test period)
December 2021 to March 2022 (Dosage)
- The first low-dose group will orally ingest test food A, and the daily intake of each ingredient will be 80 mg of glutathione.
-The second low-dose group orally ingested test food B, and the daily intake of each component was 40 mg of glutathione.
- The placebo group orally ingested the control food, and the ingredients other than glutathione were the same as Test Foods 1 and 2.

(Skin brightness measurement)
The brightness of the same spot on the face was measured over time at 0, 4, 8, and 12 weeks using a spectrophotometer (CM-26d (Konica Minolta Japan, Inc.)).

The results are shown in Figure 1 and Table 1. As a result of measuring the brightness of the arm, a significant improvement in brightness was observed in the low-dose groups 1 and 2 compared to the time at the start of intake. Furthermore, regarding the second low-dose group, the skin brightness improved even with a low dose of glutathione, which was significantly improved compared to the placebo group.

Claims (5)

An oral preparation for improving skin brightness that contains glutathione as an active ingredient. The oral preparation for improving skin brightness according to claim 1, wherein the glutathione content is 100 mg or less. The oral preparation for improving skin brightness according to claim 1 or 2, wherein the glutathione is glutathione contained in yeast extract. A method for improving skin brightness by orally ingesting 100mg/day or less of glutathione. 5. The method for improving skin brightness according to claim 4, wherein the glutathione is glutathione contained in yeast extract.