JP2024007997A - Composition for glucose metabolism activation, and product including composition for glucose metabolism activation - Google Patents

Abstract

To provide a new composition for glucose metabolism activation.SOLUTION: A composition for glucose metabolism activation contains ultrafine wire bubbles at a concentration of 1×108/mL or more as an active ingredient.SELECTED DRAWING: Figure 2

Description

The present disclosure relates to a composition for activating sugar metabolism and a product containing the composition for activating sugar metabolism.

Patent Document 1 discloses a composition for improving sugar metabolism containing a specific compound as an active ingredient.

International Publication No. 2012/014524

Conventionally, there are various substances that promote sugar metabolism, but there are concerns about side effects. In addition, methods to improve the reaction efficiency of glycolysis, citric acid cycle, etc. include the use of amino acids, sugars, and enzymes, but all of these methods require expensive materials.

The present disclosure has been made in view of the above problems, and aims to provide a new composition for activating sugar metabolism. The present disclosure can be realized as the following forms.

The composition for activating sugar metabolism of the present disclosure contains ultrafine bubbles having a concentration of 1×10 ⁸ bubbles/mL or more as an active ingredient.

According to the present disclosure, a new composition for activating sugar metabolism can be provided.

FIG. 1 is a diagram illustrating the presumed mechanism by which sugar metabolism is activated. FIG. 2 is a graph showing the relationship between UFB concentration and glucose concentration in Test 1. FIG. 3 is a graph showing the relationship between UFB concentration and glucose concentration in Test 2.

Here, a desirable example of the present disclosure will be shown.
- The composition for activating sugar metabolism contains at least one member selected from the group consisting of yeast extract, glucose, sodium chloride, dipotassium phosphate, peptone, casein-sui digested peptone, and soybean-papain digested peptone. good.
- The composition for activating sugar metabolism is selected from the group consisting of impregnation, injection, dialysis, eye drops, ear drops, enema, drinking, gargling, oral mucosal use, skin external use, nasal absorption, and inhalation. It is preferable that the dosage form is as follows.
- The composition for activating sugar metabolism can be used to treat diseases or symptoms related to sugar metabolism selected from the group consisting of disorders of sugar metabolism, diabetes, hyperglycemia, obesity, brain function, fatigue, arteriosclerosis, and immune function. It may be used to prevent, treat, or improve function.
・Products containing the composition for activating sugar metabolism are products containing the above-mentioned composition for activating sugar metabolism, and include foods, health foods, beverages, alcoholic beverages, feed, fodder, pet food, aquaculture water, It is preferably selected from the group consisting of plant growing water, culture solution, cosmetics, pharmaceuticals, and quasi-drugs.

The present disclosure will be described in detail below. In addition, in this specification, descriptions using "-" for numerical ranges include the lower limit value and the upper limit value, unless otherwise specified. For example, the description "10-20" includes both the lower limit value "10" and the upper limit value "20". That is, "10-20" has the same meaning as "10 or more and 20 or less".

1. Composition for Activating Sugar Metabolism The composition for activating sugar metabolism contains ultra fine bubbles (UFB) with a concentration of 1×10 ⁸ bubbles/mL or more as an active ingredient.

(1) Ultra-fine bubbles Ultra-fine bubbles are bubbles with a diameter of less than 1 μm (ISO 20298-1). Ultra-fine bubbles can be prepared according to known methods for producing nano-sized microbubbles. For example, it can be produced by a micropore method, a gas-liquid mixing shear method, a static mixer method, a venturi method, a cavitation method, a vapor condensation method, an ultrasonic method, a swirling flow method, a pressurized melting method, or the like. Among these, from the viewpoint of the usefulness of the obtained composition for activating sugar metabolism, a microporous method is preferable, and a microporous method using a ceramic porous material selected from silica, alumina (γ-alumina), zeolite, etc. method is more preferable.

The gas in the ultra fine bubble is not particularly limited. The gas in the ultra-fine bubble is preferably selected from the group consisting of air, water vapor, hydrogen, tritium, rare gas, oxygen, carbon dioxide, nitrogen, and fluoride gas. Examples of the rare gas include helium (He), neon (Ne), argon (Ar), krypton (Kr), xenon (Xe), and radon (Rn). Examples of the fluoride gas include CF ₄ , C ₂ F ₆ , C ₃ F ₈ , C ₄ F ₈ , CHF ₃ , SF ₆ , NF ₃ and the like. The technology of the present disclosure can be said to be a highly versatile technology that can be applied regardless of the type of gas. The gas in the ultra-fine bubbles is preferably oxygen or water vapor from the viewpoint of the usefulness of the resulting composition for activating sugar metabolism.

The liquid used in the composition for activating sugar metabolism is not particularly limited as long as it can maintain ultra-fine bubbles and is physiologically acceptable (less harmful to cells). Examples of such liquids include pure water, tap water, rainwater, fresh water, seawater, artificial seawater, physiological saline, cosmetics, drinking liquids, seasonings, etc., depending on the use of the composition for activating sugar metabolism. Alcohol, culture fluids (glucose-containing liquid, albumin-containing liquid, antibody-containing liquid, vitamin-containing liquid, serum-containing liquid, plasma-containing liquid), infusion preparations (sugar solution, hypotonic electrolyte solution (starting solution (No. 1 solution), maintenance Infusion solution (No. 3 solution)), injection solution, extracellular fluid replenisher (lactated Ringer's solution, acetic Ringer's solution), artificial colloid solution, artificial blood, buffer solution (acetate buffer, phosphate buffer, citrate buffer, citric acid) Phosphate buffer, borate buffer, tartrate buffer, Tris buffer, phosphate buffer saline, McIlvaine buffer), etc. can be used.

The ultra-fine bubble concentration (hereinafter also referred to as UFB concentration) of the composition for activating sugar metabolism is not particularly limited. The UFB concentration of the composition for activating sugar metabolism is 1×10 ⁸ cells/mL or more, preferably 1×10 ⁸ cells/mL or more and 1×10 ¹⁴ cells/mL or less. UFB concentration can be measured using a nanoparticle analysis system. An example of a nanoparticle analysis system is NanoSight (manufactured by Spectris PLC, NS-300, hereinafter simply referred to as NanoSight).

(2) Application of Composition for Activating Glucose Metabolism In the present disclosure, the activating effect on sugar metabolism refers to the effect of taking sugar (for example, glucose) into cells and promoting its consumption as an energy source. Glucose metabolism is a metabolic pathway essential for the survival of many organisms, and is composed of sugar metabolic pathways centered on glycolysis.
The technology of the present disclosure is mainly based on new findings obtained from tests using E. coli. In a test using E. coli, when the UFB concentration in the medium was 1 x 10 ⁸ cells/mL or more, the glucose concentration in the medium after culturing for a predetermined time was lower than when it was less than 1 x 10 ⁸ cells/mL. It was confirmed that this decreases. It was also confirmed that the decrease in the glucose concentration of the medium was not dependent on the dissolved oxygen concentration but on the UFB concentration. The decrease in glucose concentration in the medium is thought to be due to E. coli taking up glucose and consuming it as an energy source. Based on these findings, the present inventors discovered that ultrafine bubbles act through a mechanism different from that of dissolved gas in activating the sugar metabolism of E. coli, and developed the technology of the present disclosure. Ta.
Escherichia coli is known as a model organism that has a glycolytic system and a citric acid cycle. Many organisms share many common sugar metabolic pathways and the basic structure of their cell membranes (membrane transport proteins, liposomes, etc.). The technology of the present disclosure is applicable to microorganisms such as E. coli, and is also considered to be a highly versatile technology that can be applied to all living things including cells having sugar metabolic pathways.

The composition for activating sugar metabolism may be an ultra-fine bubble solution containing components other than ultra-fine bubbles. The composition for activating sugar metabolism can be obtained by forming gas into fine bubbles, and is revolutionary in that ultra-fine bubbles and other solutes can be used together.

Components other than ultra-fine bubbles in the composition for activating sugar metabolism are not particularly limited as long as they are physiologically acceptable components. From the viewpoint of activating sugar metabolism, the composition for activating sugar metabolism preferably contains components useful for the growth of the target organism. The composition for activating sugar metabolism contains at least one member selected from the group consisting of yeast extract, glucose, sodium chloride, dipotassium phosphate, peptone, casein-sui digested peptone, and soybean-papain digested peptone. Preferably.

In the composition for activating sugar metabolism, ultrafine bubbles may promote the uptake into cells of sugars such as glucose that are degraded during sugar metabolism. From this point of view, it is preferable that the composition for activating sugar metabolism contains sugar that is decomposed by sugar metabolism. Examples of sugars decomposed by sugar metabolism include glucose, galactose, fructose, mannose, and pentose. Note that even if the composition for activating sugar metabolism does not contain sugar, it is thought that ultrafine bubbles can act on sugar in the body and activate sugar metabolism. That is, as long as the composition for activating sugar metabolism contains at least ultra-fine bubbles, it does not necessarily need to contain sugars that are decomposed by sugar metabolism.

The subject to which the composition for activating sugar metabolism can be administered may be any organism that contains cells having a sugar metabolism pathway, such as microorganisms, animals, plants, and the like. When the subject to be administered is an animal, it may be a mammal or a human. The human subject to administration may be healthy or may be suffering from a disease related to sugar metabolism. When treatment of a disease is intended, subjects to whom the composition for activating glucose metabolism is typically administered include subjects suffering from or at risk of suffering from the disease. Diseases related to sugar metabolism include glucose metabolism disorders, diabetes, hyperglycemia, obesity, arteriosclerosis, and the like.

The composition for activating sugar metabolism is a disease, symptom, or function related to sugar metabolism selected from the group consisting of glucose metabolism disorder, diabetes, hyperglycemia, obesity, brain function, fatigue, arteriosclerosis, and immune function. can be used to prevent, treat, or improve. Diseases related to obesity include metabolic syndrome. To prevent or improve obesity, the composition for activating sugar metabolism can be used for dieting purposes.

The dosage form of the composition for activating sugar metabolism is not particularly limited. The composition for activating sugar metabolism can be expected to have an effect of activating the sugar metabolism of cells by administering the ultrafine bubbles in a manner that allows them to come into contact with cells. The composition for activating sugar metabolism is, for example, from the group consisting of impregnation, injection, dialysis, eye drops, ear drops, enema, drinking, gargling, oral mucosal use, skin external use, nasal absorption, and inhalation. The dosage form may be selected.
The state of the composition for activating sugar metabolism is not particularly limited. The state of the composition for activating sugar metabolism is assumed to be liquid, gel, or mist depending on the dosage form.

(3) Presumed mechanism The reason why the composition for activating sugar metabolism of the present disclosure can activate sugar metabolism is not clear, but it is presumed as follows. Note that the technology of the present disclosure is not interpreted to be limited by this reason for speculation.
Membrane transport proteins with pore diameters of several tens of nanometers are present in the cell membranes of living organisms (see Figure 1). Glucose dispersed in extracellular fluid is mainly taken into cells by passive transport via membrane transport proteins. Ultrafine bubbles are charged to a negative zeta potential and are known to adsorb positively charged substances. On the other hand, glucose and liposomes, which are the main components of cell membranes, are known to be positively charged substances. Therefore, it is thought that the following steps promote the uptake of glucose into cells.
Step 1: Glucose adsorbs on the UFB surface.
Step 2: UFB wrapped in glucose is adsorbed to the cell membrane surface.
Step 3: Glucose and UFB wrapped in glucose are taken into cells via membrane transport proteins.
Since sugar metabolism is a chemical reaction, it is presumed that when the amount of glucose uptake (substance amount) increases, glucose consumption is accelerated and sugar metabolism is activated.

In addition to the above-mentioned speculation mechanism, ultra-fine bubbles are electrically charged, which causes nutritional components to be electrically adsorbed to the bubble surface, causing nutrients to be locally condensed. The dispersibility in the liquid improves due to the influence of electrical charge, and the concentration distribution of nutrients becomes balanced. When cells take in nutrients through ion channels, they also take in air bubbles, and the air bubbles contribute to metabolic reactions. You can also think of an order.

2. Method for producing a composition for activating sugar metabolism The composition for activating sugar metabolism can be produced by generating ultra-fine bubbles and obtaining a liquid containing ultra-fine bubbles at a concentration of 1 x 10 ⁸ bubbles/mL or more. . When the composition for activating sugar metabolism is a solution, the composition for activating sugar metabolism can be produced by mixing a liquid containing ultra-fine bubbles at a predetermined concentration or higher and a solution containing other solutes. .

The method for producing ultra-fine bubbles is not particularly limited. Ultra-fine bubbles can be generated using, for example, a device equipped with a porous structure (element). Examples of devices equipped with a porous structure (element) include the device described in JP2018-86632A, the device described in International Publication No. 2020/004653, the device described in JP2021-154262A, etc. , the contents of which are incorporated herein.

3. Field of Application The field of industrial application of the composition for activating sugar metabolism is not particularly limited. For example, when plants are to be administered, fields such as agriculture and forestry can be mentioned. When animals are to be administered, fields such as aquaculture and livestock farming can be mentioned. When microorganisms are to be administered, examples include fields such as alcoholic beverages, fermented foods, culture, and biopharmaceuticals. In addition, when the subject is to be administered to humans, fields such as medicine, healthcare, and sports can be mentioned. In addition, cultivation applications include fields such as fermentation processes for foods and alcoholic beverages, biopharmaceuticals, and bioprocesses for regenerative medicine. In addition, for marine applications, it can be applied to aquaculture (growth promotion) of various types of fish. For agricultural and fishery purposes, it can be applied to hydroponic cultivation (growth promotion), etc. Applications to humans include treatment, beauty, health, slimming, training, and other applications that benefit from promoting sugar metabolism. Since ultrafine bubbles can be produced at low cost, they are expected to be used in various fields as compositions for activating sugar metabolism.

Products containing the composition for activating sugar metabolism are not particularly limited. The composition for activating sugar metabolism is expected to have the effect of activating the sugar metabolism of cells when used as a liquid that comes into contact with cells, such as blood, infusions, aquaculture tanks, agricultural water, and culture fluids, or when added to these liquids. can. Products containing the composition for activating sugar metabolism include foods, health foods, beverages, alcoholic beverages, feed, feedstuffs, pet foods, aquaculture water, plant cultivation water, culture solutions, cosmetics, pharmaceuticals, and quasi-drugs. You can choose from the following groups. Products containing the composition for activating sugar metabolism are expected to be used as products that are inexpensive and have the added value of activating sugar metabolism. In addition, the ultra-fine bubbles themselves are not thought to accumulate in the body, and are therefore promising as a product with fewer concerns about side effects.

The dosage form and effective dosage of pharmaceuticals and quasi-drugs using the composition for activating sugar metabolism of the present disclosure depend on the subject of administration, dosage form, patient condition, and physician's judgment. Yes, but not limited. For example, for an adult weighing 60 kg, 50 mL to 800 mL of ultra-fine bubble liquid can be administered per dose. The frequency of administration is preferably once every 8 hours to 5 days, for example.

1. Test 1
(1) Preparation of culture medium (culture solution) Using a device equipped with a porous ceramic element, ultra-fine bubbles of oxygen gas are generated in pure water using a micropore method, and water containing ultra-fine bubbles is was created.

A twice-concentrated TBS medium was prepared. The components of the TBS medium are as follows.
Components of TBS medium: casein-sui digested peptone 34.0g/L, soybean-papain digested peptone 6.0g/L, sodium chloride 10.0g/L, dipotassium phosphate 5.0g/L, glucose 5.0g/L L

Water containing ultra-fine bubbles and twice the concentration TBS medium were mixed in equal amounts to prepare media for samples 1-2 and 1-3. The culture media of samples 1-2 and 1-3 are examples of compositions for activating sugar metabolism.

A medium for sample 1-1 was prepared in the same manner as for samples 1-2 and 1-3, except that pure water was used instead of water containing ultra-fine bubbles. The medium of Sample 1-1 is a comparative example (Blank).

Optical sterilization was performed on the culture medium of each sample 1-1, 1-2, and 1-3 using UV light (wavelength: 250 nm, output: 6 W).

(2) E. coli test At Kitasato Environmental Science Center, a test was conducted to culture E. coli using the above medium. The outline of the test is as follows.
Escherichia coli (NBRC331) was inoculated into the medium of each sample at a concentration of 1×10 ⁶ CFU/mL, and was statically cultured at 36±2° C. A closed type culture container without a vent in the lid was used. 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, and 7 hours after the start of culture, the glucose concentration (mg/dL) of the medium, the total amount of adenylate contained in the medium and E. coli ( The total amount of ATP, ADP, and AMP) was measured.

2. Exam 2
(1) Preparation of medium (culture solution) The medium of sample 2-1 was prepared by aerating the medium obtained in the same manner as the medium of sample 1-1 with a diffuser tube as a treatment to increase the dissolved oxygen concentration. The medium of Sample 2-1 is a comparative example (Blank).

The medium for Sample 2-2 was prepared by vacuum degassing the medium obtained in the same manner as the medium for Samples 1-2 and 1-3 as a treatment to lower the dissolved oxygen concentration. The medium of Sample 2-2 is an example of a composition for activating sugar metabolism.

The medium for sample 2-3 was prepared in the same manner as the medium for samples 1-2 and 1-3. That is, the culture medium of Sample 2-3 was not subjected to any treatment to adjust the dissolved oxygen concentration. The medium of Sample 2-3 is an example of a composition for activating sugar metabolism.

The culture medium of each sample 2-1, 2-2, and 2-3 was sterilized by filtration using a syringe filter (membrane type: polypropylene) with a pore size of 0.22 μm. The culture medium of each sample 2-1, 2-2, and 2-3 was sealed in an aluminum pouch container and brought to the testing institution in order to suppress fluctuations in dissolved gas.

(2) E. coli test At Kitasato Environmental Science Center, a test was conducted to culture E. coli using the above medium. The outline of the test is as follows.
Escherichia coli (NBRC331) was inoculated into the culture medium of each sample at a concentration of 1×10 ⁶ CFU/mL, and was statically cultured at 36±2° C. The culture container used was an open type with a vent in the lid. 2 hours, 4 hours, 5 hours, 6 hours, 7 hours, and 24 hours after the start of culture, the glucose concentration (mg/dL) of the medium, the total amount of adenylate contained in the medium and E. coli ( The total amount of ATP, ADP, and AMP) was measured.

2. Analysis (1) Dissolved oxygen concentration The dissolved oxygen concentration of the medium of each sample before the start of culture was measured. A dissolved oxygen meter (TOA DKK DO-31P) was used for the measurement. This dissolved oxygen meter is a device that detects the dissolved oxygen concentration in a solution using a diaphragm electrode method.
(2) UFB concentration The UFB concentration (UFB cells/ml) in the medium of each sample before the start of culture was measured. A NanoSight NS-300 was used for the measurement. The measurement conditions were as follows.
[Measurement condition]
Frequency: 405nm
Camera Level: 15
Number of captures: 5
Capture duration:60
Detection Threshold: 4

(3) Glucose concentration After a predetermined time from the start of culture, the glucose concentration of the medium was measured. A glucose monitor (GlucCell manufactured by Wakenbee Tech) was used for the measurement. GlucCell is a device that detects the glucose concentration in a dropped liquid using an electrochemical biosensor (enzyme electrode method).

(4) Total amount of adenylate A predetermined time after the start of culture, the total amount of adenylate contained in the culture medium and E. coli was measured. The measurement was performed by ATP test (A3 method) using Kikkoman Lumitester PD-30. The ATP test (A3 method) is a method for detecting the total amount of ATP, ADP, and AMP. More specifically, after ADP and AMP are converted to ATP by enzymes, the total amount of ATP, ADP, and AMP contained in the sample is detected by the amount of light emitted when they react with luciferase (RLU, Relative Light Unit). do. Measurements were performed three times for each specimen.

3. Results (1) Results of Test 1 Table 1 shows the dissolved oxygen concentration (mg/mL) and UFB concentration (units/mL) of the medium of each sample before the start of culture. The UFB concentration of sample 1-2 was 1.05×10 ⁸ cells/mL. The UFB concentration of sample 1-3 was 4.44×10 ⁸ cells/mL.

Glucose concentration was measured 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, and 7 hours after the start of culture. After 6 hours, sample 1-2 and sample 1-3 The difference between the average value and sample 1-1 (Blank) was the largest. Table 1 shows the glucose concentration (mg/dL) and the turbidity of the medium (OD ₆₆₀ ) 6 hours after the start of culture.

The relationship between UFB concentration and glucose concentration 6 hours after the start of culture is shown in FIG. 2. The dotted line is a linear approximate curve obtained by the least squares method. The equation of the linear approximation curve is y=-4E-08x+110.99, and the correlation coefficient is R ² =0.9526.

6 hours after the start of culture, that is, at the time when the difference between the average value of Sample 1-2 and Sample 1-3 and the glucose concentration between Sample 1-1 (Blank) and that of Sample 1-1 (Blank) is maximum, the UFB concentration and glucose concentration are determined. It was found that there is a correlation between In addition, since the turbidity (OD ₆₆₀ ) of the culture medium of samples 1-1, 1-2, and 1-3 after 6 hours from the start of culture is the same, samples 1-1, 1-2, and 1- It is thought that the number of E. coli in sample No. 3 is approximately the same. In other words, the uptake of glucose into each of the E. coli bacteria in samples 1-1 and 1-2 was promoted more than the uptake of glucose into each of the E. coli bacteria in sample 1-3 during the period from the start of culture to 6 hours. It is inferred.

The total adenylate amount (RLU) of Sample 1-2 and Sample 1-3 6 hours after the start of culture was higher than the total adenylate amount (RLU) of Sample 1-1 (Blank). The increase in the total amount of adenylate contained in the medium and E. coli is presumed to be due to E. coli consuming glucose as an energy source and producing ATP.

Further, sample 1-3 has a higher UFB concentration than sample 1-2. The glucose concentration of sample 1-3 was lower than that of sample 1-2. Since the dissolved oxygen concentrations of Samples 1-2 and 1-3 were equivalent, it was suggested that the glucose concentration decreased not depending on the dissolved oxygen concentration but depending on the UFB concentration.

(2) Results of Test 2 Table 2 shows the dissolved oxygen concentration (mg/mL) and UFB concentration (units/mL) of the culture medium of each sample before the start of culture. The UFB concentration of sample 2-2 was 7.33×10 ⁸ cells/mL. The UFB concentration of sample 2-3 was 6.97×10 ⁸ cells/mL.

Glucose concentration was measured 2 hours, 4 hours, 5 hours, 6 hours, 7 hours, and 24 hours after the start of culture. After 7 hours, sample 2-2 and sample 2-3 The difference between the average value and sample 2-1 (Blank) was the largest. Table 2 shows the glucose concentration (mg/dL) and the turbidity of the medium (OD ₆₆₀ ) 7 hours after the start of culture.

FIG. 3 shows the relationship between UFB concentration and glucose concentration 7 hours after the start of culture. The dotted line is a linear approximate curve obtained by the least squares method. The equation of the linear approximation curve is y=-1E-08x+38.277, and the correlation coefficient is R ² =0.9189.

Seven hours after the start of culture, that is, at the time when the difference between the average value of Sample 2-2 and Sample 2-3 and the glucose concentration between Sample 2-1 (Blank) and that of Sample 2-1 (Blank) is maximum, the UFB concentration and glucose concentration are It was found that there is a correlation between In addition, since the turbidity (OD ₆₆₀ ) of the culture medium of samples 2-1, 2-2, and 2-3 after 7 hours from the start of culture is the same, samples 2-1, 2-2, and 2- It is thought that the number of E. coli in sample No. 3 is approximately the same. In other words, during the period from the start of culture to 7 hours, the uptake of glucose into each of the E. coli bacteria in samples 2-1 and 2-2 was promoted more than the uptake of glucose into each of the E. coli bacteria in sample 2-3. It is inferred.

The total adenylate amount (RLU) of Sample 2-2 and Sample 2-3 7 hours after the start of culture was higher than the total adenylate amount (RLU) of Sample 2-1 (Blank). The increase in the total amount of adenylate contained in the medium and E. coli is presumed to be due to E. coli consuming glucose as an energy source and producing ATP.

Further, sample 2-2 has a lower dissolved oxygen concentration and higher UFB than sample 2-3. The glucose concentration of sample 2-2 was lower than that of sample 2-3. This suggested that the glucose concentration decreased not depending on the dissolved oxygen concentration but depending on the UFB concentration.

From the above results, in a medium containing ultrafine bubbles at a concentration of 1 x 10 ⁸ cells/mL or more, glucose uptake into cells is promoted and sugar metabolism is activated, depending on the UFB concentration. It was suggested.

4. Effects of Examples As described above, according to this example, it was possible to provide a new composition for activating sugar metabolism, which contains ultrafine bubbles with a concentration of 1×10 ⁸ bubbles/mL or more as an active ingredient.

It should be noted that the embodiments disclosed herein are illustrative in all respects and should not be considered restrictive. The scope of the present invention is not limited to the embodiments disclosed herein, and includes all modifications within the scope indicated by the claims or within the scope equivalent to the claims. is intended.

Claims (5)

A composition for activating sugar metabolism, which contains ultrafine bubbles having a concentration of 1×10 ⁸ bubbles/mL or more as an active ingredient. The sugar metabolic activity according to claim 1, which contains at least one selected from the group consisting of yeast extract, glucose, sodium chloride, dipotassium phosphate, peptone, casein-sui digested peptone, and soybean-papain digested peptone. Composition for chemical treatment. Claim 1, wherein the administration form is selected from the group consisting of impregnation, injection, dialysis, eye drops, ear drops, enema, drinking, gargling, oral mucosal use, skin external use, nasal absorption, and inhalation. Or the composition for activating sugar metabolism according to claim 2. Preventing, treating, or improving diseases, symptoms, or functions related to glucose metabolism selected from the group consisting of glucose metabolism disorders, diabetes, hyperglycemia, obesity, brain function, fatigue, arteriosclerosis, and immune function. The composition for activating sugar metabolism according to claim 1 or 2, which is used for. A product containing the composition for activating sugar metabolism according to claim 1 or 2, which is a food, health food, beverage, alcoholic beverage, feed, fodder, pet food, culture water, plant growth water, culture solution. A product containing a composition for activating sugar metabolism selected from the group consisting of cosmetics, pharmaceuticals, and quasi-drugs.

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