JP2023553983A - 二重鎖シーケンシングのための方法 - Google Patents

二重鎖シーケンシングのための方法 Download PDF

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Publication number
JP2023553983A
JP2023553983A JP2023535673A JP2023535673A JP2023553983A JP 2023553983 A JP2023553983 A JP 2023553983A JP 2023535673 A JP2023535673 A JP 2023535673A JP 2023535673 A JP2023535673 A JP 2023535673A JP 2023553983 A JP2023553983 A JP 2023553983A
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Japan
Prior art keywords
dna
sequencing
duplex
strand
adapter
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Pending
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JP2023535673A
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English (en)
Japanese (ja)
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JP2023553983A5 (https=
Inventor
アダルステインソン，ヴィクター，エー．
ベイ，ジン
リウ，ルオリン
マクリジョーゴス，ジェラッシモス
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Broad Institute Inc
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Broad Institute Inc
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Publication of JP2023553983A publication Critical patent/JP2023553983A/ja
Publication of JP2023553983A5 publication Critical patent/JP2023553983A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023535673A 2020-12-11 2021-12-10 二重鎖シーケンシングのための方法 Pending JP2023553983A (ja)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US202063124696P 2020-12-11 2020-12-11
US63/124,696 2020-12-11
US202163143334P 2021-01-29 2021-01-29
US63/143,334 2021-01-29
US202163208951P 2021-06-09 2021-06-09
US63/208,951 2021-06-09
US202163217232P 2021-06-30 2021-06-30
US63/217,232 2021-06-30
US202163239920P 2021-09-01 2021-09-01
US63/239,920 2021-09-01
PCT/US2021/062966 WO2022125997A1 (en) 2020-12-11 2021-12-10 Method for duplex sequencing

Publications (2)

Publication Number Publication Date
JP2023553983A true JP2023553983A (ja) 2023-12-26
JP2023553983A5 JP2023553983A5 (https=) 2024-12-18

Family

ID=81974016

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023535673A Pending JP2023553983A (ja) 2020-12-11 2021-12-10 二重鎖シーケンシングのための方法

Country Status (4)

Country Link
US (1) US20240052342A1 (https=)
EP (1) EP4259820A4 (https=)
JP (1) JP2023553983A (https=)
WO (1) WO2022125997A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024206328A1 (en) * 2023-03-28 2024-10-03 Accuragen Holdings Limited Methods for duplex sequencing
WO2024200193A1 (en) * 2023-03-31 2024-10-03 F. Hoffmann-La Roche Ag Methods and compositions for dna library preparation and analysis
KR20260026601A (ko) * 2023-04-13 2026-02-26 아니링 에스엘. Dna 시퀀싱 방법
EP4709879A1 (en) * 2023-05-12 2026-03-18 Roche Sequencing Solutions, Inc. Enzymatic conversion of methylated nucleic acids for sequencing
KR102630597B1 (ko) 2023-08-22 2024-01-29 주식회사 지놈인사이트테크놀로지 종양 정보를 활용한 미세 잔존 질환 탐지 방법 및 장치

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018501776A (ja) * 2014-10-17 2018-01-25 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
JP2019532090A (ja) * 2016-10-31 2019-11-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ キメラ生成物の同定のためのバーコードを付けられた環状ライブラリーの構築
US20200115752A1 (en) * 2010-10-01 2020-04-16 Life Technologies Corporation Nucleic acid adaptors and uses thereof
JP2020511966A (ja) * 2017-03-23 2020-04-23 ユニヴァーシティ オブ ワシントン エラーが訂正された核酸配列決定への適用を伴う標的化核酸配列濃縮のための方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003298733B2 (en) 2002-11-27 2009-06-18 Agena Bioscience, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
WO2009103068A1 (en) 2008-02-15 2009-08-20 Life Technologies Corporation Methods and compositions for shearing of polymers by sonication
US20180121599A1 (en) * 2009-09-05 2018-05-03 Vito Nv (Vlaamse Instelling Voor Technologisch Onderzoek) Methods and systems for detecting a nucleic acid in a sample by analyzing hybridization
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
WO2020185967A1 (en) * 2019-03-11 2020-09-17 Red Genomics, Inc. Methods and reagents for enhanced next generation sequencing library conversion and insertion of barcodes into nucleic acids.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200115752A1 (en) * 2010-10-01 2020-04-16 Life Technologies Corporation Nucleic acid adaptors and uses thereof
JP2018501776A (ja) * 2014-10-17 2018-01-25 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
JP2019532090A (ja) * 2016-10-31 2019-11-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ キメラ生成物の同定のためのバーコードを付けられた環状ライブラリーの構築
JP2020511966A (ja) * 2017-03-23 2020-04-23 ユニヴァーシティ オブ ワシントン エラーが訂正された核酸配列決定への適用を伴う標的化核酸配列濃縮のための方法

Also Published As

Publication number Publication date
WO2022125997A1 (en) 2022-06-16
US20240052342A1 (en) 2024-02-15
EP4259820A1 (en) 2023-10-18
EP4259820A4 (en) 2024-12-11

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