JP2023547298A - クラスターごとの強度補正及びベースコールのためのシステム及び方法 - Google Patents

クラスターごとの強度補正及びベースコールのためのシステム及び方法 Download PDF

Info

Publication number
JP2023547298A
JP2023547298A JP2022580968A JP2022580968A JP2023547298A JP 2023547298 A JP2023547298 A JP 2023547298A JP 2022580968 A JP2022580968 A JP 2022580968A JP 2022580968 A JP2022580968 A JP 2022580968A JP 2023547298 A JP2023547298 A JP 2023547298A
Authority
JP
Japan
Prior art keywords
intensity
current
channel
specific
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2022580968A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023547298A5 (https=
Inventor
エリック・ジョン・オジャード
アブデ・アリ・フナイド・カガルワーラ
ラミ・メヒオ
ニティン・ウドパ
ギャヴィン・デレク・パーナビー
ジョン・エス・ヴィエチェリ
Original Assignee
イルミナ インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イルミナ インコーポレイテッド filed Critical イルミナ インコーポレイテッド
Publication of JP2023547298A publication Critical patent/JP2023547298A/ja
Publication of JP2023547298A5 publication Critical patent/JP2023547298A5/ja
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06FELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00Pattern recognition
    • G06F18/20Analysing
    • G06F18/24Classification techniques
    • G06F18/241Classification techniques relating to the classification model, e.g. parametric or non-parametric approaches
    • G06F18/2411Classification techniques relating to the classification model, e.g. parametric or non-parametric approaches based on the proximity to a decision surface, e.g. support vector machines
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06FELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00Pattern recognition
    • G06F18/20Analysing
    • G06F18/21Design or setup of recognition systems or techniques; Extraction of features in feature space; Blind source separation
    • G06F18/213Feature extraction, e.g. by transforming the feature space; Summarisation; Mappings, e.g. subspace methods
    • G06F18/2135Feature extraction, e.g. by transforming the feature space; Summarisation; Mappings, e.g. subspace methods based on approximation criteria, e.g. principal component analysis
    • G06F18/21355Feature extraction, e.g. by transforming the feature space; Summarisation; Mappings, e.g. subspace methods based on approximation criteria, e.g. principal component analysis nonlinear criteria, e.g. embedding a manifold in a Euclidean space
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/10Segmentation; Edge detection
    • G06T7/187Segmentation; Edge detection involving region growing; involving region merging; involving connected component labelling
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00Arrangements for image or video recognition or understanding
    • G06V10/40Extraction of image or video features
    • G06V10/50Extraction of image or video features by performing operations within image blocks; by using histograms, e.g. histogram of oriented gradients [HoG]; by summing image-intensity values; Projection analysis
    • G06V10/507Summing image-intensity values; Histogram projection analysis
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/30Unsupervised data analysis
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B45/00ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks

Landscapes

  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Data Mining & Analysis (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medical Informatics (AREA)
  • Artificial Intelligence (AREA)
  • Evolutionary Computation (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioethics (AREA)
  • Software Systems (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Databases & Information Systems (AREA)
  • Signal Processing (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Telephonic Communication Services (AREA)
  • Apparatus For Radiation Diagnosis (AREA)
  • Nuclear Medicine (AREA)
  • Complex Calculations (AREA)
JP2022580968A 2020-10-27 2021-10-26 クラスターごとの強度補正及びベースコールのためのシステム及び方法 Pending JP2023547298A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063106256P 2020-10-27 2020-10-27
US63/106,256 2020-10-27
PCT/US2021/056711 WO2022093865A1 (en) 2020-10-27 2021-10-26 Systems and methods for per-cluster intensity correction and base calling

Publications (2)

Publication Number Publication Date
JP2023547298A true JP2023547298A (ja) 2023-11-10
JP2023547298A5 JP2023547298A5 (https=) 2024-12-06

Family

ID=78822676

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022580968A Pending JP2023547298A (ja) 2020-10-27 2021-10-26 クラスターごとの強度補正及びベースコールのためのシステム及び方法

Country Status (10)

Country Link
US (5) US11361194B2 (https=)
EP (2) EP4459625A3 (https=)
JP (1) JP2023547298A (https=)
KR (1) KR20230098097A (https=)
CN (1) CN115803817A (https=)
AU (1) AU2021369495B2 (https=)
CA (1) CA3183529A1 (https=)
IL (1) IL298884A (https=)
MX (1) MX2022016354A (https=)
WO (1) WO2022093865A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11361194B2 (en) * 2020-10-27 2022-06-14 Illumina, Inc. Systems and methods for per-cluster intensity correction and base calling
KR20250073048A (ko) 2022-09-16 2025-05-27 일루미나, 인코포레이티드 클러스터 분할 및 조건부 염기 호출
WO2024124379A1 (zh) * 2022-12-12 2024-06-20 深圳华大智造科技股份有限公司 一种多模板核酸同步测序的方法及其应用
KR102949918B1 (ko) 2023-11-21 2026-04-07 전남대학교산학협력단 Dna 저장장치의 오류 정정을 위한 실시간 염기서열 자동 분석 장치 및 방법
US20250210137A1 (en) * 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
WO2025190902A1 (en) 2024-03-13 2025-09-18 Illumina, Inc. Improving base calling quality scores

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180195953A1 (en) * 2017-01-06 2018-07-12 Illumina, Inc. Phasing correction
US20210310065A1 (en) * 2013-12-03 2021-10-07 Illumina, Inc. Methods and systems for analyzing image data

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2073908A (en) 1930-12-29 1937-03-16 Floyd L Kallam Method of and apparatus for controlling rectification
WO2000063437A2 (en) 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6865301B1 (en) 2000-02-28 2005-03-08 Adobe Systems Incorporated Reducing aliasing artifacts when shaping a digital image
CA2309002A1 (en) 2000-05-23 2001-11-23 Jonathan Martin Shekter Digital film grain reduction
US6778692B1 (en) 2000-08-11 2004-08-17 General Electric Company Image processing method and apparatus including image improving circuit
US6598013B1 (en) 2001-07-31 2003-07-22 University Of Maine Method for reducing cross-talk within DNA data
US7664326B2 (en) 2004-07-09 2010-02-16 Aloka Co., Ltd Method and apparatus of image processing to detect and enhance edges
WO2006015251A2 (en) 2004-07-29 2006-02-09 The Research Foundation Of State University Of New York System and method for cross-talk cancellation in a multilane fluorescence detector
US9445025B2 (en) 2006-01-27 2016-09-13 Affymetrix, Inc. System, method, and product for imaging probe arrays with small feature sizes
CA2689626C (en) 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
US8703422B2 (en) 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2009142856A1 (en) * 2008-04-18 2009-11-26 Trustees Of Boston University Direction sensitive neutron detector
US8407012B2 (en) 2008-07-03 2013-03-26 Cold Spring Harbor Laboratory Methods and systems of DNA sequencing
US20100034444A1 (en) 2008-08-07 2010-02-11 Helicos Biosciences Corporation Image analysis
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
US20120015825A1 (en) 2010-07-06 2012-01-19 Pacific Biosciences Of California, Inc. Analytical systems and methods with software mask
EP2643783A2 (en) * 2010-11-22 2013-10-02 Life Technologies Corporation Model-based residual correction of intensities
WO2013040583A2 (en) 2011-09-16 2013-03-21 Complete Genomics, Inc Determining variants in a genome of a heterogeneous sample
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
EP3836149A1 (en) 2011-11-07 2021-06-16 QIAGEN Redwood City, Inc. Methods and systems for identification of causal genomic variants
EP3366348B1 (en) 2012-01-16 2023-08-23 Greatbatch Ltd. Emi filtered co-connected hermetic feedthrough, feedthrough capacitor and leadwire assembly for an active implantable medical device
US8906320B1 (en) 2012-04-16 2014-12-09 Illumina, Inc. Biosensors for biological or chemical analysis and systems and methods for same
US20140235456A1 (en) 2012-12-17 2014-08-21 Virginia Tech Intellectual Properties, Inc. Methods and Compositions for Identifying Global Microsatellite Instability and for Characterizing Informative Microsatellite Loci
US9665694B2 (en) 2013-01-31 2017-05-30 Codexis, Inc. Methods, systems, and software for identifying bio-molecules with interacting components
WO2014182630A1 (en) 2013-05-06 2014-11-13 Pacific Biosciences Of California , Inc. Real-time electronic sequencing
DK3017065T3 (en) 2013-07-01 2018-11-26 Illumina Inc Catalyst-free Surface functionalization and polymer grafting
EP3084002A4 (en) 2013-12-16 2017-08-23 Complete Genomics, Inc. Basecaller for dna sequencing using machine learning
CN106462670B (zh) 2014-05-12 2020-04-10 豪夫迈·罗氏有限公司 超深度测序中的罕见变体召集
AU2015332389A1 (en) 2014-10-16 2017-04-20 Myriad Women's Health, Inc. Variant caller
WO2016066586A1 (en) 2014-10-31 2016-05-06 Illumina Cambridge Limited Novel polymers and dna copolymer coatings
JP2019505884A (ja) 2015-12-10 2019-02-28 キアゲン ゲーエムベーハー デジタル画像内の少なくとも1つのオブジェクトの全体的明るさを判定するための方法
US10038862B2 (en) 2016-05-02 2018-07-31 Qualcomm Incorporated Methods and apparatus for automated noise and texture optimization of digital image sensors
US10467749B2 (en) 2016-10-10 2019-11-05 Genemind Biosciences Company Limited Method and system for processing an image comprising spots in nucleic acid sequencing
NL2018852B1 (en) 2017-05-05 2018-11-14 Illumina Inc Optical distortion correction for imaged samples
US11288576B2 (en) * 2018-01-05 2022-03-29 Illumina, Inc. Predicting quality of sequencing results using deep neural networks
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
US11210554B2 (en) * 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
US12062417B2 (en) 2019-05-01 2024-08-13 New York University System, method and computer accessible-medium for multiplexing base calling and/or alignment
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2021081129A1 (en) 2019-10-21 2021-04-29 Illumina, Inc. Increased calculation efficiency for structured illumination microscopy
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
US11361194B2 (en) * 2020-10-27 2022-06-14 Illumina, Inc. Systems and methods for per-cluster intensity correction and base calling
CN118511225A (zh) * 2021-09-02 2024-08-16 华盛顿大学 多重时间分辨分子信号记录器及相关方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210310065A1 (en) * 2013-12-03 2021-10-07 Illumina, Inc. Methods and systems for analyzing image data
US20180195953A1 (en) * 2017-01-06 2018-07-12 Illumina, Inc. Phasing correction

Also Published As

Publication number Publication date
US20220129711A1 (en) 2022-04-28
WO2022093865A1 (en) 2022-05-05
EP4238098C0 (en) 2024-08-21
EP4459625A2 (en) 2024-11-06
CN115803817A (zh) 2023-03-14
IL298884A (en) 2023-02-01
US20240220584A1 (en) 2024-07-04
CA3183529A1 (en) 2022-05-05
US11593595B2 (en) 2023-02-28
EP4238098A1 (en) 2023-09-06
US20230259588A1 (en) 2023-08-17
EP4238098B1 (en) 2024-08-21
AU2021369495B2 (en) 2026-04-02
US12216741B2 (en) 2025-02-04
US11853396B2 (en) 2023-12-26
US20250265313A1 (en) 2025-08-21
MX2022016354A (es) 2023-01-24
AU2021369495A1 (en) 2023-02-02
EP4459625A3 (en) 2025-01-22
US20220300772A1 (en) 2022-09-22
KR20230098097A (ko) 2023-07-03
US11361194B2 (en) 2022-06-14

Similar Documents

Publication Publication Date Title
JP2023547298A (ja) クラスターごとの強度補正及びベースコールのためのシステム及び方法
JP7767672B2 (ja) ベースコールのための等化器ベースの強度補正
KR20240022490A (ko) 뉴클레오티드 염기 호출 및 염기 호출 품질을 결정하기 위한 신호-대-잡음비 메트릭
JP2025526537A (ja) フローセル傾斜に対する塩基コーリングの依存性
EP4483372B1 (en) Calibration sequences for nucelotide sequencing
HK40114649A (en) Systems and methods for per-cluster intensity correction and base calling
HK40090027A (en) Systems and methods for per-cluster intensity correction and base calling
HK40090027B (en) Systems and methods for per-cluster intensity correction and base calling
US20240266003A1 (en) Determining and removing inter-cluster light interference
US20240177807A1 (en) Cluster segmentation and conditional base calling
US20250210137A1 (en) Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
WO2025240924A1 (en) Blind equalization systems for base calling applications

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241028

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20241028

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241128

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20251104

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260204