JP2023125051A - Medical equipment for injecting cell suspension into target site - Google Patents

Abstract

To provide medical equipment capable of appropriately administering cells to a target site with a simple mechanism and easy operation.SOLUTION: There is provided medical equipment for injecting a cell suspension into a target site, which includes a support part for supporting a puncture part perpendicularly to a body surface, and a puncture part for injecting a cell suspension, and is configured so that the puncture part can be deviated perpendicularly to the body surface.SELECTED DRAWING: Figure 1

Description

The present invention relates to a medical device for injecting a cell suspension into a target site.

In recent years, attempts have been made to transplant various cells to repair damaged tissues and the like. For example, use of fetal cardiomyocytes, skeletal myoblasts, mesenchymal stem cells, cardiac stem cells, ES cells, iPS cells, etc. to repair myocardial tissue damaged by ischemic heart diseases such as angina pectoris and myocardial infarction. has been attempted (Non-Patent Document 1).
As part of such efforts, cell structures formed using scaffolds and sheet-shaped cell cultures in which cells are formed into sheets have been developed (Non-Patent Document 2). Furthermore, another example of repairing damaged tissues, etc. by transplantation of cells, etc. includes CD34-positive cell therapy for severe lower limb diseases. In such treatment, CD34-positive cells are dissolved in physiological saline and injected intramuscularly into dozens of locations in both legs that are in a severe ischemic state (Non-Patent Document 3).

Patent Document 1 describes a liquid injection device. Such a liquid injection device includes a cylindrical body whose inner cavity contains a liquid, a hollow needle disposed at the distal end of the cylindrical body, communicating with the cylindrical body, and having a sharp needle tip at the distal end thereof; an inner structure having a gasket that can slide within the cylinder; a pusher connected to the gasket to move and operate the gasket along the longitudinal direction of the cylinder; on the outer peripheral side of the inner structure; A protector is arranged to be movable along the axial direction with respect to the inner structure and has a tubular shape with an open tip, and by pressing the tip of the protector against the target area, the protector moves in the proximal direction. It is described that the tip of the hollow needle moves, the needle tip of the hollow needle is exposed, the engagement between the pusher and the elastic piece is released, and the pusher pushes out the liquid in the inner cavity and injects it.

Patent No. 6038020

Haraguchi et al., Stem Cells Transl Med. 2012 Feb;1(2):136-41 Sawa et al., Surg Today. 2012 Jan;42(2):181-4 Fujita et al., Circulation Journal Vol.78, February 2014: 490-501

When administering cells into a tissue as described above, the operations of appropriately positioning and administering the cells are repeated at each administration site. As a result, the time required to administer a sufficient amount of cells becomes longer, and the long-term administration operation places a great burden on the recipient. Therefore, an object of the present invention is to provide a medical device that can solve these problems and appropriately administer cells to a target site with a simple mechanism and simple operations.

While conducting intensive research to solve the above problems, the present inventor discovered that the above problems could be solved by providing a medical device equipped with a support section that positions the puncture by the puncture section, and based on this knowledge, As a result of further research based on the above, the present invention has been completed.

That is, the present invention relates to the following.
[1] A medical device for injecting a cell suspension into a target site,
a puncture part for injecting a cell suspension, and a support part that supports the puncture part perpendicularly to the body surface;
The medical device, wherein the puncture section is configured to be displaceable in a direction perpendicular to the body surface.
[2] The medical device according to [1], further including an elastic body, and the elastic body is configured to return the puncture portion displaced in a direction perpendicular to the body surface to its original position.
[3] The medical device according to [1] or [2], further comprising a storage section that stores a cell suspension, and the storage section is configured to deliver the cell suspension to the puncture site. .
[4] The medical device according to [3], further comprising a supply section in fluid communication with the reservoir, the supply section being configured to supply the cell suspension to the reservoir.

[5] The storage part is configured to deliver the cell suspension to the puncture part by being pressed, and to supply the cell suspension from the supply part by weakening the pressure, [4] Medical devices listed in .
[6] The medical device according to any one of [1] to [5], wherein the target site is tissue surrounding a blood vessel.
[7] A method of injecting a cell suspension into a target site, comprising:
The puncture part includes a puncture part for injecting a cell suspension and a support part that supports the puncture part perpendicularly to the body surface, and the puncture part is configured to be displaceable in the direction perpendicular to the body surface. The step of providing medical equipment that has been
The method described above, comprising: bringing the tip of the support into contact with the body surface; and displacing the puncture in a direction perpendicular to the body surface.

According to the present invention, the simple operation of bringing the tip of the support part into contact with the body surface located directly above the target area to properly position the part and displacing the puncture part in a direction perpendicular to the body surface The cell suspension can then be properly injected.
Further, according to the present invention, after the cell suspension is injected into the target site, preparations for reinjection can be easily made, so that it is possible to continuously inject the cell suspension into a plurality of target sites. As a result, the time required for the entire injection operation of the cell suspension can be shortened, and the burden of the injection operation of the target cell suspension can also be reduced.

FIG. 1 is a conceptual diagram of a medical device according to a first embodiment of the present invention. FIG. 2 is a conceptual diagram showing an example of use of the medical device according to the first embodiment of the present invention.

In one aspect, the present invention includes a support part that supports the puncture part perpendicularly to the body surface, and a puncture part for injecting a cell suspension, and the puncture part is displaceable perpendicularly to the body surface. The present invention relates to a medical device for injecting a cell suspension into a target site, which is configured as follows.

In the present invention, the term "cell suspension" refers to cells suspended in any medium. The cells may be in the form of a single cell, or the cells may be connected to each other via an intervening substance. A cell may contain any gene or a viral vector containing any gene, or the like. Moreover, the cell may be a cell culture. In the present invention, the cell culture refers to what is obtained through a cell culture step, and includes, but is not limited to, spheroids, sheet-shaped cell cultures, and fragments of sheet-shaped cell cultures.
In the present invention, media for suspending cells include, but are not limited to, water, physiological saline, media, buffers, diluents, cell preservation solutions, and swelling bodies such as gels.

In the present invention, the term "sheet-shaped cell culture" refers to a cell culture in which cells are connected to each other to form a sheet. Cells may be linked to each other directly (including through cellular elements such as adhesion molecules) and/or via intervening substances. The intervening substance is not particularly limited as long as it can connect cells at least physically (mechanically), and examples thereof include an extracellular matrix. The intervening substance is preferably of cellular origin, in particular of cells constituting a cell culture. The cells are at least physically (mechanically) linked, but may be further functionally, eg, chemically or electrically linked. Sheet-shaped cell cultures may be composed of one cell layer (monolayer) or two or more cell layers (laminated (multilayer), e.g., two-layer, three-layer, etc.). 4 layers, 5 layers, 6 layers, etc.). Further, the sheet-like cell culture may have a three-dimensional structure in which the cells do not exhibit a clear layered structure and have a thickness exceeding the thickness of one cell. For example, in a vertical section of a sheet-shaped cell culture, the cells may not be uniformly aligned in the horizontal direction but may be present in a non-uniformly (eg, mosaic) arrangement.

Sheet cell cultures are preferably scaffold-free. Scaffolds may be used in the art to attach cells onto and/or within their surfaces and maintain the physical integrity of sheet-like cell cultures, such as polyvinylidene difluoride ( PVDF) membranes are known, but the sheet-like cell culture of the present invention can maintain its physical integrity even without such scaffolds. Moreover, the sheet-shaped cell culture of the present invention preferably consists only of substances derived from cells constituting the sheet-shaped cell culture, and does not contain any other substances.

In the present invention, the term "crushed pieces of a sheet-shaped cell culture" refers to pieces that are divided into a plurality of crushed pieces by crushing a sheet-shaped cell culture. Although the size of the fragments is not limited to this, it is preferable that most of the fragments are of a size that does not enter the microvessel and can pass through the inside of the injection needle. For example, the average length of the diagonal line of the sheet-shaped plane is 30 μm to 1000 μm, preferably 100 μm to 500 μm. In the present invention, disruption can be carried out by any known method as long as it is possible to obtain crushed pieces of the sheet-shaped cell culture, for example, by using a syringe and needle or pipette, etc. This can be done by suspending the medium.

The cells contained in the cell suspension of the present invention are not particularly limited as long as they produce humoral factors, and include, for example, adherent cells (adherent cells). Adherent cells include, for example, adhesive somatic cells. Examples of somatic cells include myoblasts (e.g., skeletal myoblasts, etc.), muscle satellite cells, mesenchymal stem cells (e.g., bone marrow, adipose tissue, peripheral blood, skin, hair roots, muscle tissue, intrauterine cells, etc.). membranes, placenta, umbilical cord blood-derived, etc.), tissue stem cells such as cardiomyocytes, fibroblasts, and cardiac stem cells, embryonic stem cells, pluripotent stem cells such as iPS (induced pluripotent stem) cells, synovial cells, and chondrocytes. , epithelial cells (e.g., oral mucosal epithelial cells, retinal pigment epithelial cells, nasal mucosal epithelial cells, etc.), endothelial cells (e.g., vascular endothelial cells, etc.), hepatocytes (e.g., hepatic parenchymal cells, etc.), pancreatic cells (e.g., pancreatic islet cells, etc.), renal cells, adrenal gland cells, periodontal ligament cells, gingival cells, periosteal cells, skin cells, etc. Somatic cells may be those differentiated from iPS cells (iPS cell-derived cells), such as iPS cell-derived cardiomyocytes, fibroblasts, myoblasts, epithelial cells, endothelial cells, hepatocytes, pancreatic cells, and kidney cells. Examples include cells, adrenal gland cells, periodontal ligament cells, gingival cells, periosteal cells, skin cells, synovial cells, and chondrocytes. Cells include floating cells. Examples of floating cells include T lymphocytes and B lymphocytes.
Preferably, the cells contained in the cell suspension of the present invention are cells capable of promoting angiogenesis, for example cells capable of secreting factors promoting angiogenesis, for example cytokines such as VEGF.

In the present invention, the term "target site" refers to a site in a target tissue into which a cell suspension is injected. Examples of the target site in the tissue include a site where damage (wound) is present in a living body and the vicinity thereof. Damage (wound) includes, but is not limited to, damage to blood vessels, stenosis, and in such cases, the target site in the tissue is in the tissue adjacent to the blood vessel, preferably in the wall of the blood vessel. and the surrounding organization. In one embodiment, the site of interest in the tissue is a vascular sheath around the vascular tissue. The target site in the tissue may be determined in advance by ultrasonic examination, CT examination, etc. before injection.

In the present invention, the cell suspension is placed within a tissue of a subject, preferably within a perivascular tissue of a subject, more preferably within a perivascular tissue near a lesion site of a subject having a lower extremity disease. injected into. In one embodiment, the cell suspension is injected into the tissue surrounding the vessels in a patient with peripheral arterial disease at a lesion site where blood flow is impaired, such as in the adductor femoralis muscle group of the lower extremity. In one embodiment, the cell suspension is injected into the perivascular tissue near the lesion site to increase blood flow, e.g., by 35%, 40%, 45% compared to a healthy state, e.g., 7 days after injection. Flow rate can be improved. In one embodiment, the cell suspension is injected into the perivascular tissue near the lesion site to increase blood flow by, for example, about 1.5 times, about 2 times as compared to a control saline solution. , it can be improved by about 2.5 times.

In one embodiment, the subject to whom the cell suspension is injected by the medical device of the invention has a lower extremity disease. Diseases of the lower extremities include any diseases that affect the lower extremities. Examples of such diseases include, but are not limited to, peripheral artery disease, varicose veins, deep vein thrombosis, and diabetic foot lesions. Preferably, the lower extremity disease in the present invention is a disease in which blood vessels in the lower extremities are narrowed or occluded, resulting in worsening blood flow in the lower extremities, such as peripheral artery disease. In the present invention, peripheral arterial diseases include, but are not limited to, lower extremity arteriosclerosis obliterans, Buerger's disease, collagen disease, etc., and in particular severe lower extremity arteriosclerosis obliterans are treated as severe lower extremity ischemia. It is called.
Without being bound by a particular theory, the effect of the cell suspension injected by the medical device of the present invention on lower limb diseases is due to the fact that it is contained in the liquid containing humoral factors produced by the administered cells. This is thought to be due to cytokines such as VEGF acting directly and/or indirectly on cells at the ischemic site to promote angiogenesis.

In the present invention, the "puncture section" refers to a member for puncturing the body surface and injecting a cell suspension into a target site. The puncture part is a needle-like member having a lumen through which the cell suspension can pass.
In the present invention, the "support part" refers to a member that supports the puncture part perpendicularly to the body surface. The support part has a cylindrical structure on the distal end side that can fix the position of the support part with respect to the body surface. For example, the support part has a planar structure on the distal end side, and by bringing this surface into contact with the body surface, the position of the support part can be fixed with respect to the body surface. Thereby, the support part can be positioned so that the puncture part, which is supported perpendicularly to the body surface, is located directly above the target site. The support part may not directly support the puncture part, but may indirectly support the puncture part via another member. In order to properly support the puncture part against the body surface, one puncture part may be supported by a plurality of support parts. Thereby, even if the body surface has a curved surface such as an arm or a leg, the support section can appropriately support the puncture section perpendicularly to the body surface directly above the target site. In the present invention, the body surface directly above the target site refers to the position on the body surface where the puncture section is inserted perpendicularly to the body surface and the tip of the puncture section reaches the target site or the vicinity of the target site when advanced. means. In this disclosure, the target site side of the medical device of the present invention is referred to as the distal end side, and the operator side is referred to as the proximal end side.

In one aspect, the support part of the present invention is displaceable relative to other members in a direction perpendicular to the body surface. By displacing the support portion in such a direction, the tip of the puncture portion can be brought closer to or separated from the body surface while the tip of the support portion is in contact with the body surface. Moreover, by displacing the support part in such a direction, it is possible to puncture the body surface with the tip of the support part, or to remove the punctured tip of the support part. An example of such a variable structure is a structure in which the support part has a double cylindrical structure and one tube (inner tube) can be slid into the other tube (outer tube). can be mentioned. In such a structure, an elastic body can be disposed between the upper end of the lumen of the outer cylinder and the upper outer end of the inner cylinder. In this case, by applying force so that the elastic body is strained and sliding the inner tube into the outer tube, shortening the overall length of the support section, the puncture section can be directed toward the body surface. It can be displaced in the direction perpendicular to . Furthermore, by weakening the force applied to the elastic body, the elastic body tends to return to its original shape, and the inner tube is slid and pushed out from inside the outer tube by the elastic body. Thereby, the total length of the support part is increased, and the puncture part can be displaced away from the body surface in a direction perpendicular to the body surface.

In the present invention, the term "reservoir" refers to a member that stores a cell suspension. The storage section can be in fluid communication with the lumen of the puncture section on the proximal side of the puncture section, and can deliver the cell suspension stored in the storage section to the puncture section. Examples of the material for the storage section include a flexible material that can change shape. Such materials include, but are not particularly limited to, flexible resins such as polyvinyl chloride, polyethylene, and polypropylene. In one embodiment, the reservoir section can deliver the cell suspension to the puncture section by being deformed by applying force, such as by pressing. The reservoir may have a valve structure that prevents backflow of liquid from the puncture.

In the present invention, the "supply section" refers to a member that supplies the cell suspension to the storage section. The supply is in aseptic fluid communication with the reservoir and can deliver the cell suspension from the supply to the reservoir. The supply section is not particularly limited as long as it has a shape that can accommodate the fluid; for example, the supply section has a bag-like shape. When the storage section is bag-shaped, the material may be, for example, a flexible material that can change shape. Such materials include, but are not particularly limited to, flexible resins such as polyvinyl chloride, polyethylene, and polypropylene. In such a case, the supply section can supply the cell suspension to the storage section by being pressed. The supply section may have a valve structure that prevents backflow of liquid from the storage section.

In another aspect, the invention includes a puncture section for injecting a cell suspension, and a support section that supports the puncture section perpendicularly to the body surface, the puncture section being perpendicular to the body surface. providing a medical device configured to be displaceable, contacting a distal end of the support with a body surface, and displacing the puncture perpendicular to the body surface to a target site; Concerning a method for injecting a cell suspension.
The medical device provided in the step of providing a medical device of the method of the invention may be any medical device described herein. Therefore, the method of the present invention includes the steps of: injecting the cell suspension stored in the storage section from the tip of the puncture section; returning the puncture section that has been displaced in a direction perpendicular to the body surface to its original position; The method may include steps such as supplying a cell suspension from a supply to a reservoir. The injection of the cell suspension in the present invention may be continuous injection of the cell suspension into a plurality of target sites.

Hereinafter, a medical device 1 according to a preferred embodiment of the present invention will be described. FIG. 1 shows a conceptual diagram of a first embodiment of the present invention. Note that in each figure in the present disclosure, the size of each member is appropriately emphasized for ease of explanation, and the illustrated member does not indicate its actual size.

As shown in FIG. 1, the medical device 1 includes a puncture section 2 and a support section 3 that supports the puncture section 2. The support part 3 is configured to support the puncture part 2 in a direction perpendicular to the body surface, and by bringing the tip of the support part 3 into contact with the body surface, the tip of the puncture part 2 is brought into contact with the body surface. can be used to puncture the body surface from a vertical direction. The support part 3 can have a double cylindrical structure so that it can be displaced in a direction perpendicular to the body surface, and this structure allows the support part 3 to be displaced over its entire length. As a result, even when the support section 3 supports the puncture section 2 such that the distal end of the puncture section 2 is located on the proximal side of the distal end of the support section 3, the overall length of the support section 3 can be shortened. This allows the tip of the puncture section 2 to appear closer to the tip than the tip of the support section 3. An elastic body (A) 8 can be placed inside the cylindrical portion of the support portion 3 . Thereby, the overall length of the support portion 3 can be adjusted by applying force to strain the elastic body (A) 8 and by releasing the force to restore the strain in the elastic body (A) 8.

The support section 3 can support the puncture section 2 via the elastic body (B) 9. That is, as shown in FIG. 1, the elastic body (B) 9 is connected to the proximal end of the puncture section 2 and the proximal end of the support section 3 so as to be strained only in the direction perpendicular to the body surface. The puncture section 2 makes a wound through the elastic body (B) 9 so that it can slide only in a direction perpendicular to the body surface. As a result, the puncture section 2 applies force to strain the elastic body (B) 9, and releases the force to return the strain in the elastic body (B) 9, thereby causing the puncture section 2 to move against the support section 3. It can be displaced in a direction perpendicular to the body surface.

Medical device 1 can further include reservoir 4 . The storage section 4 is in fluid communication with the lumen of the puncture section 2 on the proximal end side thereof, and is arranged so that the cell suspension stored in the storage section 4 can be delivered to the puncture section 2. There is. For example, when the storage section 4 is pressed, it changes its shape and delivers the cell suspension to the puncture section 2 . As shown in FIG. 1, the storage section 4 can have an elastic body (C) 10 disposed on its proximal end side. In such a case, the storage section 4 can be pressed and delivered the cell suspension to the puncture section 2 by applying force to and straining the elastic body (C) 10. To make the elastic body (C) 10 more resistant to distortion than the elastic body (A) 8 and the elastic body (B) 9, for example, the elastic body (A) 8 and the elastic body (B) 9 can be used as the material of the elastic body (C) 10. By using a spring with a larger spring coefficient than the above, the medical device 1 is configured so that the elastic body (C) 10 is strained and the storage section 4 is pressed only after the tip of the puncture section 2 reaches the target site. be able to.

The medical device 1 can further include a supply section 5 . The supply section 5 is in fluid communication with the reservoir section 4 and is arranged so that the cell suspension can be supplied from the supply section 5 to the reservoir section 4 . For example, when an elastic body (C) is connected to the proximal end of the storage section 4, the storage section 4 that is pressed by the strain of the elastic body (C) returns to its original state when the strain of the elastic body (C) returns. The cell suspension can be configured to be supplied from the supply section 5 to the storage section 4 by utilizing the change in the internal pressure of the storage section 4 at that time when the cell suspension tries to return to the shape of the cell suspension.

The medical device 1 can further include a grip part 6 and a main body part 7 to facilitate operation. The grip part 6 is located on the proximal end side of the medical device 1, and is configured such that the puncture part 2 can be inserted into or removed from the body surface by operating the grip part 6. Moreover, the grip part 6 is configured so that force can be applied to all of the elastic body (A) 8, elastic body (B) 9, and elastic body (C) 10 through each member by pushing it. You can also do that. In such a case, by adjusting the strain susceptibility of the elastic body (A) 8, elastic body (B) 9, and elastic body (C) 10, for example, the spring coefficient of the material of each elastic body, the strain of the elastic body can be reduced. The order can be determined. For example, by setting the spring coefficient of the elastic body (A) 8<the spring coefficient of the elastic body (B)9<the spring coefficient of the elastic body (C)10, the tip of the puncture section 2 approaches the body surface. It is possible to realize the process of puncturing the surface and arriving at the target site, and administering the cell suspension from the tip. The same effect can also be achieved by setting the spring coefficient of the elastic body (B) 9<the spring coefficient of the elastic body (A) 8<the spring coefficient of the elastic body (C) 10. Further, in the medical device 1, the depth at which the puncture section 2 punctures can be adjusted by adjusting the degree of distortion of the elastic body (A) 8 and the elastic body (B) 9 and the overall length of the support section 3. In particular, when injecting a cell suspension into tissue surrounding a blood vessel using the medical device 1, the medical device 1 can puncture the body surface with the puncture part 2 in a direction perpendicular to the body surface. By measuring the distance from to the vicinity of the target blood vessel and adjusting the puncture depth of the puncture section 2 so as to puncture only that distance, the tip of the puncture section 2 can easily reach the target site. The main body part 7 is arranged so as to connect the inside of the main body part to each member or via another member so that each member can be displaced in a direction perpendicular to the body surface. The supply section 5 may be configured not to be housed in the main body section 7 but to be connected to the storage section 4 in a sterile manner only when necessary.

In order to inject a cell suspension into a target site using the medical device 1 shown in FIG. 1, first, as shown in FIG. Touch the tip of the device to the body surface directly above the target area. Next, by pushing the gripping part 6 toward the target area, the elastic body (A) 8 is strained in the direction perpendicular to the body surface, and the inner cylinder of the support part 3 is moved to the outer cylinder, as shown in FIG. 2b. By sliding into the support part 3 and shortening the overall length of the support part 3, the puncture part 2 is displaced in a direction perpendicular to the body surface, and the tip of the puncture part 2 approaches the body surface directly above the target site.
By further pushing the gripping part 6 toward the target site, the elastic body (B) 9 is strained in a direction perpendicular to the body surface, and the puncture part 2 is further displaced in a direction perpendicular to the body surface, as shown in FIG. 2c. do. Thereby, the puncture section 2 punctures the body surface, and the tip of the puncture section 2 reaches the target site. After the tip of the puncture section 2 reaches the target site, by further pushing the grip section 6, the elastic body (C) 10 is distorted in a direction perpendicular to the body surface, as shown in FIG. 2d. Thereby, the force applied to the elastic body (C) 10 is transmitted to the storage section 4 connected to the elastic body (C) 10, and the storage section 4 is pressed. The storage section 4 is deformed by pressure to deliver the cell suspension stored therein to the puncture section 2, and the cell suspension is injected from the tip of the puncture section 2 that has reached the target site.

When the injection of the cell suspension is finished, by weakening the force pushing the gripping part 6, the elastic body (C) 10, the elastic body (B) 9, and the elastic body (A) 8 are strained in this order. is returning. As a result, first of all, the pressure on the storage section 4 is removed, and the cell suspension is no longer delivered from the storage section 4 to the puncture section 2. Next, since the force pressing the puncture part 2 against the body surface is removed, the puncture part 2 is displaced away from the target site in a direction perpendicular to the body surface as the elastic body (B) 9 returns. , its tip is removed from the body surface. Finally, as the elastic body (A) 8 returns, the inner tube of the support section 3 slides so as to be pushed out from the outer tube, and the total length of the support section 3 becomes longer, so that the puncture section 2 is attached to the body surface. 2a, and is displaced away from the body surface in a direction perpendicular to the object, returning to the state shown in FIG. 2a.
Furthermore, the medical device 1 may further include a supply section 5. In such a case, the cell suspension is supplied from the supply part 5 to the storage part 4 when the medical device 1 returns to the state of FIG. 2a.

That is, use of the medical device of the present invention can be performed sequentially through the following steps.
(1) Provide medical device 1.
(2) Bring the tip of the support part 3 into contact with the body surface directly above the target area.
(3) Displace the puncture section 2 in a direction perpendicular to the body surface and puncture the body surface with the tip of the puncture section 2.
(4) Inject the cell suspension into the target site from the tip of the puncture section 2.
(5) Remove the tip of the puncture section 2 from the body surface.
In the above procedure, step (2) involves displacing the puncture part 2 in a direction perpendicular to the body surface to bring the tip of the puncture part 2 close to the body surface, and then moving the puncture part 2 to the body surface. The distal end of the puncture section 2 may be further displaced in a direction perpendicular to the above, and the tip of the puncture section 2 may be punctured into the body surface. The method may further include steps such as returning the puncture section 2 that has been displaced in a direction perpendicular to the body surface to its original position, and supplying the cell suspension from the supply section 5 to the storage section 4 .

As described above, according to the medical device of the present invention, the distal end of the support part is brought into contact with the body surface located directly above the target site for proper positioning, and the puncture part is displaced in a direction perpendicular to the body surface. The cell suspension can be properly injected by a simple operation. Further, according to the present invention, after the cell suspension is injected into the target site, preparations for reinjection can be easily made, so that it is possible to continuously inject the cell suspension into a plurality of target sites. As a result, the time required for the entire injection operation of the cell suspension can be shortened, and the burden of the injection operation of the target cell suspension can also be reduced.

1 Medical device 2 Puncture section 3 Support section 4 Storage section 5 Supply section 6 Grip section 7 Main body section 8 Elastic body (A)
9 Elastic body (B)
10 Elastic body (C)

Claims (7)

A medical device for injecting a cell suspension into a target site,
a puncture part for injecting a cell suspension, and a support part that supports the puncture part perpendicularly to the body surface;
The medical device, wherein the puncture section is configured to be displaceable in a direction perpendicular to the body surface. The medical device according to claim 1, further comprising an elastic body, the elastic body being configured to return the puncture portion displaced in a direction perpendicular to the body surface to its original position. The medical device according to claim 1 or 2, further comprising a reservoir for storing a cell suspension, the reservoir being configured to deliver the cell suspension to the puncture site. 4. The medical device of claim 3, further comprising a supply in fluid communication with the reservoir, the supply configured to supply the cell suspension to the reservoir. According to claim 4, the storage part is configured to deliver the cell suspension to the puncture part when pressed, and to be supplied from the supply part when the pressure is weakened. Medical equipment. The medical device according to any one of claims 1 to 5, wherein the target site is tissue surrounding a blood vessel. A method of injecting a cell suspension into a target site, the method comprising:
The puncture part includes a puncture part for injecting a cell suspension and a support part that supports the puncture part perpendicularly to the body surface, and the puncture part is configured to be displaceable in the direction perpendicular to the body surface. The step of providing medical equipment that has been
The method described above, comprising: bringing the tip of the support into contact with the body surface; and displacing the puncture in a direction perpendicular to the body surface.