JP2023089690A - Mite control agent - Google Patents

Abstract

To provide a control agent for Balaustium murorum (Hermann).SOLUTION: A control agent for Balaustium murorum (Hermann) contains ant trail pheromone as an active ingredient.SELECTED DRAWING: None

Description

Applied for application of Article 30, Paragraph 2 of the Patent Act (Part 1) Date of publication on website September 13, 2021 Website address https://acarology-japan. org/taikai/30-2021/ja/https://acarology-japan. org/taikai/30-2021/ja/program/https://acarology-japan. org/taikai/30-2021/images/30th_program_F. pdf (Part 2) Date September 17, 2021 to September 19, 2021 (Released on September 18, 2021) Meeting name, venue The 30th Annual Meeting of the Japanese Society of Ticks (online (Zoom))

The present disclosure relates to acaricides and the like.

Balaustium murorum (Hermann), also called red mites, is known as a nuisance pest that occurs from spring to summer because it moves around quickly. Recommended methods for exterminating Takara mites include washing with water, wiping with adhesive, filling gaps in the concrete where the mites are generated, removing pollen that feeds on them (removing flowers), and using insecticides. The method suffers from the fact that it may be impractical and its effect is temporary. In addition, although miticides are said to have little adverse effect on useful insects such as natural enemy insects and pollinator insects, the problem is that the dead traces of the miticide killed by miticide treatment remain as red stains. Therefore, the development of an effective control method that repels ticks without killing them is desired.

Japanese Patent Publication No. 2011-516484 JP-A-11-140357

Janssen et al. (1997) Nakamura et al. (2019)

An object of the present invention is to provide a control agent for Takara mite.

The present inventors have found that the Takara mite is a signpost pheromone of the mystic ant (Pristomyrmex punctatus), 6-amyl-2-pyrone (Non-Patent Document 1), and a signpost pheromone of the Japanese brown ant (Tetramorium tsushimae), 6- We found that it repels methyl methylsalicylate (Non-Patent Document 2), and made further improvements.

The present disclosure includes subject matter described, for example, in the following sections.
Section 1.
Takara mite control agent containing ant signpost pheromone as an active ingredient.
Section 2.
The ant is at least one species of ant selected from the group consisting of the following: Item 1. Takara mite control agent according to item 1.
Item 3.
Item 3. The Takara mite control agent according to Item 1 or 2, wherein the ants are Myrophila ants and/or Japanese brown ants.
Section 4.
The ant signpost pheromone is 6-amyl-2-pyrone, methyl 6-methylsalicylate, 2,5-dimethylpyrazine, 3-ethyl-2,5-dimethylpyrazine, 2,3,5-trimethylpyrazine, 2- methylpyrazine, 3-butyl-6-methyloctahydroindolizine, 2(5′-hexenyl)-5-pentylpyrrolidine, 4-methyl-3-heptanone, 1-phenylethanol, 4-methylpyrrole-2-carboxylic acid , 2-phenylacetic acid, Z-9-hexadecenal, dolichodial, and melein, the Takara mite control agent according to any one of items 1 to 3, which is at least one selected from the group consisting of.
Item 5.
A Takara mite control agent containing at least one selected from the group consisting of 6-amyl-2-pyrone and methyl 6-methylsalicylate.

A Takara mite control agent is provided.

FIG. 2 shows a T-shaped filter paper with a stem length and arm length of 35 mm and a width of 4 mm, which was used for verification of repellent activity. FIG. 3 is a diagram showing a glass petri dish on which octopus thread is stretched and a T-shaped filter paper placed in the same petri dish used for verification of repellent activity. The number of individuals reaching the end of the arm (sample side) coated with 6-amyl-2-pyrone and the end of the arm (control side) coated with solvent is shown. * in the figure indicates statistical significance at a significance level of 5%, and *** indicates statistical significance at a significance level of 0.1%. The number of individuals reaching the end of the arm (sample side) coated with methyl 6-methylsalicylate and the end of the arm (control side) coated with solvent is shown. ** indicates statistical significance at a significance level of 1% and *** indicates statistical significance at a significance level of 0.1%.

Each embodiment included in the present disclosure will be described in further detail below.
The Takara mite control agent included in the present disclosure contains an ant signpost pheromone as an active ingredient. In this specification, the control agent may be referred to as "the control agent of the present disclosure".

As used herein, the term “Takara mite” means a tick classified into the family Acarinae, Acarinae, Suborder Acarinae. As the Takara mite, ticks belonging to the genus Anatacari are preferred, and Balaustium murorum (Hermann) is more preferred.

As used herein, a "signpost pheromone" is secreted from an exocrine gland present in the abdomen or leg of an ant, and guides a large number of nest-mate ants to a feeding ground by moving them all at once, and directs them in a certain direction. A pheromone that promotes movement.
Signpost pheromones are secreted from the fugal glands at the tips of the legs of ants, and are different in composition and ecological function from substances mainly composed of long-chain hydrocarbons (footprint substances).

Ants from which signpost pheromones are derived include, for example,
Amyme ants such as Pristomex punctatus;
Wrinkled ants such as Tetramorium tsushimae from Japan, Tetramorium caespitum, Tetramorium impurum, Tetramorium caespitum, and Tetramorium meridionale;
Hymearius such as Monomorium pharaonis;
Myrmica rubra, Myrmica ruginodis, Myrmica rugulosa, Myrmica sabuleti, Myrmica scabrinodis, Myrmica sulcinodis, etc.;
Aphaenogaster albisetosus, Aphaenogaster cockerelli, etc.;
Pheidole pallidula, etc.;
leaf-cutting ants such as Atta laevigara;
Argentine ants, such as Argentine ants (Linepithema humile);
Porcupine spp. such as Formica rufa;
European black ants (Lasius fuliginosus), Japanese black ants (Lasius fuji), etc., and the like. Among them, ants that prey on Takara mite are preferable. More specifically, the genus Pistomyrmex and the genus Pistomyelia are preferred, and Pistomyrmex punctatus and Tetramorium tsushimae from Japan are more preferred.
The ant from which the signpost pheromone is derived may be of one species alone or in combination of two or more species.

６－メチルサリチル酸メチルは、日本産トビイロシワアリ（Ｔｅｔｒａｍｏｒｉｕｍ ｔｓｕｓｈｉｍａｅ）、及びＴｅｔｒａｍｏｒｉｕｍ ｉｍｐｕｒｕｍの道しるべフェロモンとして知られている。

２，５－ジメチルピラジン（ＤＭＰ）は、ヨーロッパトビイロシワアリ（Ｔｅｔｒａｍｏｒｉｕｍ ｃａｅｓｐｉｔｕｍ）、Ｔｅｔｒａｍｏｒｉｕｍ ｃａｅｓｐｉｔｕｍ、及びＴｅｔｒａｍｏｒｉｕｍ ｍｅｒｉｄｉｏｎａｌｅの道しるべフェロモンとして知られている。

３－エチル－２，５－ジメチルピラジン（ＥＤＭＰ）は、Ｔｅｔｒａｍｏｒｉｕｍ ｃａｅｓｐｉｔｕｍ、Ｔｅｔｒａｍｏｒｉｕｍ ｍｅｒｉｄｉｏｎａｌｅ、キイロクシケアリ（Ｍｙｒｍｉｃａ ｒｕｂｒａ）、Ｍｙｒｍｉｃａ ｒｕｇｉｎｏｄｉｓ、Ｍｙｒｍｉｃａ ｒｕｇｕｌｏｓａ、Ｍｙｒｍｉｃａ ｓａｂｕｌｅｔｉ、Ｍｙｒｍｉｃａ ｓｃａｂｒｉｎｏｄｉｓ、Ｍｙｒｍｉｃａ ｓｕｌｃｉｎｏｄｉｓ、及びＰｈｅｉｄｏｌｅ ｐａｌｌｉｄｕｌａの道しるべフェロモンとして知られている。

２，３，５－トリメチルピラジン（ＴＭＰ）は、Ｔｅｔｒａｍｏｒｉｕｍ ｍｅｒｉｄｉｏｎａｌｅの道しるべフェロモンとして知られている。

２－メチルピラジン（ＭＰ）は、Ｔｅｔｒａｍｏｒｉｕｍ ｍｅｒｉｄｉｏｎａｌｅの道しるべフェロモンとして知られている。

３－ブチル－６－メチルオクタヒドロインドリジン（モノモリンＩとも称される）は、イエヒメアリ（Ｍｏｎｏｍｏｒｉｕｍ ｐｈａｒａｏｎｉｓ）の道しるべフェロモンとして知られている。

２（５’－ヘキセニル）－５－ペンチルピロリジン（モノモリンＩＩＩとも称される）は、イエヒメアリ（Ｍｏｎｏｍｏｒｉｕｍ ｐｈａｒａｏｎｉｓ）の道しるべフェロモンとして知られている。

４－メチル－３－ヘプタノンは、Ａｐｈａｅｎｏｇａｓｔｅｒ ａｌｂｉｓｅｔｏｓｕｓの道しるべフェロモンとして知られている。

１－フェニルエタノールは、Ａｐｈａｅｎｏｇａｓｔｅｒ ｃｏｃｋｅｒｅｌｌｉの道しるべフェロモンとして知られている。

４－メチルピロール－２－カルボン酸は、Ａｔｔａ ｌａｅｖｉｇａｒａの道しるべフェロモンとして知られている。

２－フェニル酢酸は、Ａｔｔａ ｌａｅｖｉｇａｒａの道しるべフェロモンとして知られている。

Ｚ－９－ヘキサデセナールは、アルゼンチンアリ（Ｌｉｎｅｐｉｔｈｅｍａ ｈｕｍｉｌｅ）の道しるべフェロモンとして知られている。

ドリコジアールは、アルゼンチンアリ（Ｌｉｎｅｐｉｔｈｅｍａ ｈｕｍｉｌｅ）の道しるべフェロモンとして知られている。

メレインは、アカヤマアリ（Ｆｏｒｍｉｃａ ｒｕｆａ）及びヨーロッパクロクサアリ（Ｌａｓｉｕｓ ｆｕｌｉｇｉｎｏｓｕｓ）の道しるべフェロモンとして知られている。

本開示の防除剤は、道しるべフェロモンである上述した成分を含むことが好ましい。これらの成分は、１種単独で又は２種以上を組み合わせて用いることができる。中でも、６－アミル－２－ピロン、６－メチルサリチル酸メチルが好ましい。 6-Amyl-2-pyrone is known as a beacon pheromone for mysid ants (Pristomyrmex punctatus).

Methyl 6-methylsalicylate is known as a beacon pheromone for Japanese brown ants (Tetramorium tsushimae) and Tetramorium impurum.

2,5-Dimethylpyrazine (DMP) is known as a beacon pheromone for Tetramorium caespitum, Tetramorium caespitum, and Tetramorium meridionale.

3-Ethyl-2,5-dimethylpyrazine (EDMP) is a product of Tetramorium caespitum, Tetramorium meridionale, Myrmica rubra, Myrmica ruginodis, Myrmica rugulosa, Myrmica sabuleti, M Known as a signpost pheromone for yrmica scabrinodis, Myrmica sulcinodis, and Pheidole pallidula It is

2,3,5-trimethylpyrazine (TMP) is known as a landmark pheromone in Tetramorium meridionale.

2-Methylpyrazine (MP) is known as a landmark pheromone in Tetramorium meridionale.

3-Butyl-6-methyloctahydroindolizine (also called Monomorin I) is known as a guide pheromone for the house ant (Monomorium pharaonis).

2(5′-hexenyl)-5-pentylpyrrolidine (also called monomorin III) is known as a guide pheromone for the house ant (Monomorium pharaonis).

4-Methyl-3-heptanone is known as a landmark pheromone for Aphaenogaster albisetosus.

1-Phenylethanol is known as the trailblazer pheromone of Aphaenogaster cockerelli.

4-Methylpyrrole-2-carboxylic acid is known as the trailblazer pheromone of Atta laevigara.

2-Phenylacetic acid is known as the signpost pheromone of Atta laevigara.

Z-9-Hexadecenal is known as a beacon pheromone for Argentine ants (Linepithema humile).

Dolichodiale is known as the beacon pheromone of the Argentine ant (Linepithema humile).

Mellaine is known as a beacon pheromone for the red wood ant (Formica rufa) and the European black ant (Lasius fuliginosus).

Control agents of the present disclosure preferably include the aforementioned components that are signpost pheromones. These components can be used individually by 1 type or in combination of 2 or more types. Among them, 6-amyl-2-pyrone and methyl 6-methylsalicylate are preferred.

The components mentioned above may be of synthetic origin or may be of natural origin.

The content of the signpost pheromone contained in the control agent of the present disclosure is not particularly limited, and can be appropriately set, for example, in the range of 0.01 to 100% by mass.

Control agents of the present disclosure contain the components described above and may further contain other components. Such other components include, for example, pharmaceutically acceptable bases, carriers, additives (e.g., solvents, dispersants, emulsifiers, buffers, stabilizers, excipients, binders, lubricants, antioxidants, agents, preservatives, etc.) and the like. These components can be used individually by 1 type or in combination of 2 or more types.

The control agent of the present disclosure can be prepared by a conventional method by combining the components described above and, if necessary, other components.

The form of the control agent of the present disclosure is not particularly limited, and includes solid forms such as powder, granules, tablets, and capsules; semi-solid or liquid forms such as liquid, emulsion, and gel. be able to.

The control agent of the present disclosure can be used to control Takara mite. In addition, since the control agent of the present disclosure has a repellent effect on Takara mite, it can be more suitably used as a Takara mite repellent. Therefore, the control agent of the present disclosure can control Takara mite, which is a nuisance pest, without imposing a large burden on the environment.

In general, mites have a short life history and quickly acquire resistance to control agents, so many of them do not maintain the effects of control agents. The control agent of the present disclosure contains a guidepost pheromone for ants that prey on Takara mite as an active ingredient (more specifically, a repellent ingredient). more likely to be predated by human ants. Therefore, it is predicted that drug resistance to the same component is difficult to develop. Therefore, it is expected that long-term use and repeated use will become possible.
Footprints are left in the footprints of ants, but Takara ticks have not been confirmed to avoid walking tracks (including footprints) of predators, mysid ants. Since Takara ticks move quickly, pheromones that indicate the risk of encountering a large number of ants, rather than footprints that show traces of their activity, are pheromones that indicate the risk of encountering a large number of ants. It is considered effective as an active ingredient in repellents).

The control agent of the present disclosure is applied to, for example, plants such as crops or fruit trees, soils for cultivating them, facility materials (for example, vinyl houses and glass greenhouses) that serve as migration routes for Takara mite, house walls, concrete surfaces, etc. be able to.

The control agent of the present disclosure may be applied as it is, or may be applied after being suspended in a solvent such as water.

The application method of the control agent of the present disclosure is not particularly limited, and can be appropriately set according to the form of the control agent of the present disclosure. Examples thereof include spraying on plants, soil, facility materials, house walls, concrete surfaces, and the like.

The application amount of the control agent of the present disclosure is not particularly limited, and can be appropriately set according to the form, application target, etc. of the control agent of the present disclosure.

In this specification, the term "comprising" includes "consisting essentially of" and "consisting of." Also, the present disclosure encompasses any and all combinations of the constituent elements described herein.

Also, the various characteristics (property, structure, function, etc.) described for each of the embodiments of the disclosure described above may be combined in any way to identify subject matter encompassed by the disclosure. That is, the present disclosure encompasses all subject matter consisting of any and all possible combinations of the features described herein.

The contents of the present disclosure will be specifically described using the following experimental examples. However, the present disclosure is by no means limited to these. In the following, experiments are conducted under atmospheric pressure and normal temperature conditions, unless otherwise specified.

Individuals of Balaustium murorum (Hermann; hereinafter abbreviated as Takara mite) living in concrete areas such as the sides of buildings in the Saga Campus of Kyoto Institute of Technology were captured using a very fine face brush. To prevent escape, the rats were placed in a plastic container whose inner wall surface was coated with a Teflon (registered trademark) suspension solution: Fluon made by Asahi Glass, kept in a fasting state, and used for experimental verification within 6 hours after capture.

The chemicals used as validation samples were 6-amyl-2-pyrone (Tokyo Kasei), a guidepost pheromone for Mystic ants (Pristomyrmex punctatus), and 6-methylsalicylate (Tetramorium tsushimae), a guidepost pheromone from Japan. Synthetic products).

To verify the repellent activity against Takara mite, a T-shaped filter paper with a total width of 2 mm, a stem length and an arm length of 35 mm was prepared (Fig. 1), and 5 µL of a hexane solution containing a test sample was placed on one arm (sample side), and 5 µL on the other arm. 5 μL of solvent hexane was applied to the arm (control side), air-dried, and then used for the repellency test.

After three black threads were stretched around a glass petri dish with a diameter of 90 mm (Fig. 2, left), a T-shaped filter paper was placed on the threads after adjusting so that the ends of the stem and arms protruded by about 2 mm (Fig. 2). right). Using an ultra-fine brush, a Takara tick was placed on the end of the stem, its behavior was observed, and selection behavior at the branch point of the arm was recorded. Those that reached the end of the arm were determined as selected individuals. The application amount (absolute amount) of the test sample is set based on the content of each signpost pheromone per individual in Amyme ants and Tobiiroshiwa ants, and 6-amyl-2-pyrone per arm (17.5 mm length) of T-shaped filter paper was 200 pg (1 solid equivalent (AE)), 2 ng (10 solid equivalent (AE)); methyl 6-methylsalicylate was 300 pg (0.1 solid equivalent (AE)), 3 ng (1 solid equivalent (AE)) . The results are shown in FIG. 3 (6-amyl-2-pyrone) and FIG. 4 (methyl 6-methylsalicylate).

As shown in FIGS. 3 and 4, it was confirmed that Takara mite repels 6-amyl-2-pyrone and methyl 6-methylsalicylate.

In addition, it was not confirmed that Takara ticks tend to avoid footprints of mysid ants.

Claims (5)

Takara mite control agent containing ant signpost pheromone as an active ingredient. The ant is at least one species of ant selected from the group consisting of the following: Takara mite control agent according to claim 1. 3. The Takara mite control agent according to claim 1 or 2, wherein the ants are Amyme ants and/or Japanese brown ants. The ant signpost pheromone is 6-amyl-2-pyrone, methyl 6-methylsalicylate, 2,5-dimethylpyrazine, 3-ethyl-2,5-dimethylpyrazine, 2,3,5-trimethylpyrazine, 2- methylpyrazine, 3-butyl-6-methyloctahydroindolizine, 2(5′-hexenyl)-5-pentylpyrrolidine, 4-methyl-3-heptanone, 1-phenylethanol, 4-methylpyrrole-2-carboxylic acid , 2-phenylacetic acid, Z-9-hexadecenal, dolichodial, and at least one selected from the group consisting of melein, Takara mite control agent according to any one of claims 1 to 3. A Takara mite control agent containing at least one selected from the group consisting of 6-amyl-2-pyrone and methyl 6-methylsalicylate.

Images