JP2022096765A - Cell transplantation apparatus, and method for using cell transplantation apparatus - Google Patents

Abstract

To provide a cell transplantation apparatus capable of efficiently forming a peripheral environment that can induce tissue formation from a cell group, and a method for using the cell transplantation apparatus.SOLUTION: A cell transplantation apparatus includes: a needle-like part 10 extending cylindrically, having an opening 11 at an end part, and configured to be capable of housing a transplant inside the needle-like part 10; and an inner member 30 provided with a rod part 31 extending inward of the needle-like part 10. A rod-like part 31 is configured to be relatively movable to the needle-like part 10 between a position where an end part is arranged inside the needle-like part 10 and a position where the end part projects from the opening 11 of the needle-like part 10. The inner member 30 has: a separation part 32 including the end part of the rod-like part 31; and a support part 33 supporting the base end part of the separation part 32, the separation part 32 being configured to be separable from the support part 33. The cell transplantation apparatus is suitably used for transplantation of a transplant including a cell group to an organism.SELECTED DRAWING: Figure 3

Description

The present invention relates to a cell transplant device and a method of using the cell transplant device.

The use of technology for transplanting cell groups into living organisms is advancing. For example, attempts have been made to regenerate hair by culturing a group of cells that contribute to the formation of hair follicles, which are organs that produce hair, and transplanting the group of cells into the skin. For good hair regeneration, it is desirable that the transplanted cell population yields hair follicles with normal tissue structure and good hair-forming ability. Therefore, various researches and developments have been carried out on a method for producing a cell group capable of forming such hair follicles (see, for example, Patent Documents 1 to 3).

Further, a cell transplantation device, which is a device for transferring a cultured cell group from a culture vessel into a living body, is being developed (see, for example, Patent Document 4).

International Publication No. 2017/073625 International Publication No. 2012/108069 Japanese Unexamined Patent Publication No. 2008-29331 International Publication No. 2019/064653

In order to obtain desired results such as tissue regeneration by transplanting a cell group, it is important that tissue formation from the cell group arranged in the living body progresses well. For example, when a group of cells that contribute to the formation of hair follicles is transplanted, the hair follicles are formed up to the surface layer of the skin, and the hair formed at the base of the hair follicles is formed so that the hair comes out from the surface layer of the skin. Is desirable.

The environment in which the cell group is arranged in the living body is one of the factors that influence the tissue formation based on the cell group. Therefore, it is preferable that the surrounding environment of the cell group can induce good tissue formation from the cell group. If such a surrounding environment can be formed by a cell transplantation device, it is possible to efficiently arrange the cell group in the living body and form the surrounding environment.

Therefore, a cell transplantation device capable of forming a surrounding environment capable of inducing tissue formation from a cell group is desired.

The cell transplantation device for solving the above-mentioned problems is a transplantation including a group of cells which is a needle-shaped portion extending in a cylindrical shape, has an opening at the tip of the needle-shaped portion, and is a target of transplantation into a living body. The needle-shaped portion is configured to accommodate an object inside, and an inner member having a rod-shaped portion extending inside the needle-shaped portion is provided. The inside of the rod-shaped portion is configured to be relatively movable with respect to the needle-shaped portion between a position arranged inside the portion and a position where the tip portion of the rod-shaped portion protrudes from the opening of the needle-shaped portion. The member has a separating portion including the tip end portion of the rod-shaped portion and a supporting portion that supports the base end portion of the separating portion, and the separating portion is configured to be separable from the supporting portion.

According to the above configuration, the cell transplantation device can place the transplanted portion and the separated portion with respect to the living body through the opening of the needle-shaped portion. As a result, the separation part is arranged so as to extend from the inside of the living body toward the surface, so that tissue formation along the separation part is induced from the cell group. Therefore, it is possible to form a surrounding environment that can induce tissue formation from the cell group. Then, according to the cell transplantation apparatus, since the separation part that induces tissue formation can be arranged with respect to the living body together with the transplant, a member for inducing tissue formation is placed at the transplant site separately from the arrangement of the transplant. It is possible to easily and efficiently arrange the cell group in the living body and form the surrounding environment as compared with the arrangement in the living body.

In the above configuration, the rod-shaped portion may be a tubular structure.
According to the above configuration, the rod-shaped portion including the separation portion has a function of inducing tissue formation after placement in a living body and a function of holding the transplanted material when the transplanted material is taken into the needle-shaped part. It can play a role in assisting the transplantation of transplants. Therefore, the transplantation of the transplant can proceed smoothly.

In the above configuration, the rod-shaped portion may be a columnar structure having no fluid flow path inside.
According to the above configuration, the rod-shaped portion has a shape more suitable for inducing tissue formation than the case where the rod-shaped portion has a cylindrical shape. Therefore, the surrounding environment of the cell group can be more preferably formed.

In the above configuration, the rod-shaped portion is a columnar structure having no fluid flow path inside, and the inner member is a tubular structure extending in parallel with the rod-shaped portion inside the needle-shaped portion. It may be provided with a tubular portion.

According to the above configuration, the rod-shaped portion has a shape more suitable for inducing tissue formation than the case where the rod-shaped portion has a cylindrical shape. Further, the tubular portion can be used to hold the implant when the implant is taken into the needle-shaped portion. Since the functions of the rod-shaped portion and the tubular portion are separated, the rod-shaped portion and the tubular portion can be configured to be more suitable for each function, so that the transplant can be retained and the cell group can be held. Each of the formation of the surrounding environment can be performed accurately.

In the above configuration, the tubular portion, which is the tubular structure, is connected to a suction portion that sucks the inside of the tubular portion, and the tubular portion is of the tubular portion by suction in the tubular portion. The implant may be configured to be able to be held at the tip portion.
According to the above configuration, since the transplant can be stably held, it is possible to smoothly take in the transplant into the needle-shaped portion and transport the taken-in transplant.

In the above configuration, each of the separation portion and the support portion is a part of the rod-shaped portion, and the tip end portion of the support portion may be connected to the base end portion of the separation portion.
According to the above configuration, it is possible to easily realize a configuration of a support portion that supports the separated portion in a separable manner.

In the above configuration, the rod-shaped portion may have a fragile portion at a position including the base end portion of the separating portion, which is a portion having a lower strength against an external force than other portions of the rod-shaped portion.
According to the above configuration, the separated portion can be separated by applying an external force to the rod-shaped portion to break the fragile portion. Therefore, the separation portion can be easily separated.

In the above configuration, the cross-sectional area of the fragile portion may be 90% or less of the average cross-sectional area of the support portion.
According to the above configuration, since the fragile portion is easily broken by an external force, the separated portion can be smoothly separated.

In the above configuration, the entire rod-shaped portion may be the separation portion.
According to the above configuration, it is possible to shorten the length of the rod-shaped portion as compared with the form in which a part of the rod-shaped portion is a separated portion. Therefore, the formation of a minute rod-shaped portion becomes easy.

In the above configuration, the cell transplantation apparatus includes a separation forming portion including a mechanism for separating the separating portion from the supporting portion, and the separation forming portion is such that the entire separating portion is entirely from the opening of the needle-shaped portion. It may be configured to separate the separation portion in a protruding state.

According to the above configuration, a mechanism for separating the separating portion is arranged outside the structure having the needle-shaped portion. Therefore, the degree of freedom regarding the structure, size, movement, etc. of the mechanism is large. Therefore, it is easy to configure the mechanism suitable for dividing the rod-shaped portion, and the separation of the separated portion can be smoothly promoted.

In the above configuration, the cell transplantation apparatus includes a separation forming portion including a mechanism for separating the separating portion from the supporting portion, and the separation forming portion has the needle-shaped portion at the base end portion of the separating portion. It may be configured to separate the separation portion without coming out of the opening.
According to the above configuration, it is possible to shorten the length of the rod-shaped portion as compared with the form in which the separated portion is separated with the entire separated portion protruding from the opening of the needle-shaped portion. Therefore, it becomes easy to form a minute rod-shaped portion.

In the above configuration, the needle-shaped portion may be configured to accommodate the transplant containing the cell group, which is a cell aggregate having a diameter of 100 μm or more and 1000 μm or less.
According to the above configuration, the cell transplantation device becomes a device suitable for transplantation of a cell group that contributes to hair growth or hair growth such as a hair follicle primordium.

In the above configuration, the cell transplantation apparatus may be used for transplanting the transplant containing the cell group, which is a cell aggregate containing skin-derived stem cells, into a living body.
According to the above configuration, by using the cell transplantation device, the separation part is arranged with respect to the skin of the living body together with the transplantation containing the cell group that contributes to hair growth or hair growth such as the hair follicle primordium. Then, the separated portion extends from the vicinity of the transplant toward the skin surface, and the end portion of the separated portion protrudes from the skin, so that hair follicles are formed up to the skin surface layer, and the formed hairs come out of the skin. Tissue formation can be induced so as to proceed.

The method of using the cell transplant device for solving the above-mentioned problems is to take the transplant into the inside of the needle-shaped portion, pierce the needle-shaped portion into a living body, and then attach the separated portion and the transplanted product. It includes taking out from the opening of the needle-shaped portion and separating the separating portion from the supporting portion.

According to the above method, the implant and the separation part are arranged with respect to the living body through the opening of the needle-shaped part. As a result, the separation part is arranged so as to extend from the inside of the living body toward the surface, so that tissue formation along the separation part is induced from the cell group. In this way, since the separation part that induces tissue formation can be arranged in the living body together with the transplant, the arrangement of the cell group in the living body and the formation of the surrounding environment can be easily and efficiently performed. be able to.

According to the present invention, it is possible to efficiently form a surrounding environment capable of inducing tissue formation from a cell group.

The figure which shows the whole structure of the cell transplantation apparatus of 1st Embodiment. The figure which shows the structure of the cell transplantation apparatus which is the cell transplantation apparatus of 1st Embodiment and includes a plurality of needle-like portions. The figure which shows the internal structure of the cell transplantation apparatus of 1st Embodiment. The figure which shows the take-up process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the take-up process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the take-up process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the arrangement process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the arrangement process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the separation process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the separation process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the modification of the separation process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the modification of the separation process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the modification of the separation process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment. The figure which shows the internal structure of the cell transplantation apparatus which is the cell transplantation apparatus of 1st Embodiment, and includes the inner member which has a fragile part. It is a figure which shows the separation process using the cell transplantation apparatus which is the separation process of the transplantation in the method of using the cell transplantation apparatus of 1st Embodiment, and has an inner member which has a fragile part. The figure which shows the whole structure of the cell transplantation apparatus of 2nd Embodiment. The figure which shows the take-up process of the transplantation in the method of using the cell transplantation apparatus of 2nd Embodiment. The figure which shows the separation process of the transplantation in the method of using the cell transplantation apparatus of 2nd Embodiment. The figure which shows the internal structure of the cell transplantation apparatus which is the cell transplantation apparatus of 2nd Embodiment and includes the inner member which has a fragile part. The figure which shows the whole structure of the cell transplantation apparatus of 3rd Embodiment. The figure which shows the take-up process of the transplantation in the method of using the cell transplantation apparatus of 3rd Embodiment. The figure which shows the separation process of the transplantation in the method of using the cell transplantation apparatus of 3rd Embodiment. The figure which shows the internal structure of the cell transplantation apparatus which is the cell transplantation apparatus of 3rd Embodiment and includes the inner member which has a fragile part.

(First Embodiment)
A first embodiment of a cell transplantation apparatus and a method of using the cell transplantation apparatus will be described with reference to FIGS. 1 to 15. The cell transplantation apparatus of this embodiment is used for transplanting a transplant containing a cell group into a living body. The target area for transplantation is at least one of intracutaneous and subcutaneous, or in a tissue such as an organ. In the present embodiment, the "living body" includes not only the body or tissue of an organism but also an artificial biological model that imitates the body or tissue. That is, the cell transplantation apparatus of the present embodiment can not only transplant a transplant into an organism, but also transplant the transplant into a biological model.

[Transplant]
The cell group to be transplanted includes a plurality of cells. The cell group may be an aggregate of a plurality of aggregated cells, or may be an aggregate of a plurality of cells bound by cell-cell binding. Alternatively, the cell group may be composed of a plurality of dispersed cells. Further, the cell included in the cell group may be an undifferentiated cell or a cell whose differentiation has been completed, and the cell group may include an undifferentiated cell and a differentiated cell. good. The cell group is, for example, a cell mass (spheroid), a primordium, a tissue, an organ, or the like.

The cell group has the ability to act on tissue formation by being placed in the target area for transplantation in vivo. An example of such a cell group is a cell aggregate containing skin-derived stem cells. The cell population contributes to hair growth or hair growth, for example by being placed intradermally or subcutaneously. These cell groups have the ability to function as a hair follicle organ, differentiate into a hair follicle organ, induce or promote the formation of a hair follicle organ, or induce or promote the formation of hair in a hair follicle. .. In addition, the cell group may include cells that contribute to the control of coat color, such as pigment cells or stem cells that differentiate into pigment cells.

Specifically, an example of a cell group in this embodiment is a hair follicle primordium, which is a primitive hair follicle organ. Hair follicle primordium contains mesenchymal cells and epithelial cells. In the hair follicle organ, hair follicle head cells, which are mesenchymal cells, induce the differentiation of hair follicle epithelial stem cells, and hair matrix cells repeat division in the hair bulb formed thereby to form hair. To. Hair follicle primordium is a group of cells that differentiate into these hair follicle organs.

The hair follicle primordia is, for example, a mixture of mesenchymal cells derived from mesenchymal tissue such as the hair papilla and epithelial cells derived from epithelial tissue located in the bulge region or the base of the hair bulb under predetermined conditions. It is formed by culturing. However, the method for producing a hair follicle primordium is not limited to the above example. Further, the origin of the mesenchymal cells and epithelial cells used for producing the hair follicle primordia is not limited, and these cells may be cells derived from the hair follicle organ, and the hair follicle organ is a cell. It may be a cell derived from a different organ or a cell derived from a pluripotent stem cell.
In addition, the transplant may include a member that protects the cell group and a member that assists the transplantation of the cell group together with the cell group.

[Cell transplant device]
The overall configuration of the cell transplantation apparatus will be described with reference to FIGS. 1 and 2.
As shown in FIG. 1, the cell transplantation device 100 includes a needle-shaped portion 10 extending in a cylindrical shape and a housing portion 20 that supports a base end portion of the needle-shaped portion 10.

The needle-shaped portion 10 extends along one direction and has an opening 11 at its tip. In other words, the needle-shaped portion 10 has a hollow needle shape. The outer shape of the needle-shaped portion 10 is not particularly limited as long as the tip portion of the needle-shaped portion 10 has a shape capable of piercing a target site for transplantation such as skin in a living body. The needle-shaped portion 10 has, for example, a shape in which the tip portion of the cylinder is cut diagonally with respect to the extending direction thereof, and the tip portion of the needle-shaped portion 10 is sharp.

The housing portion 20 partitions a space inside the housing portion 20 that can accommodate the constituent members of the cell transplantation device 100. The outer shape of the housing portion 20 is not particularly limited. The housing portion 20 may be formed integrally with the needle-shaped portion 10, or may be a member formed separately from the needle-shaped portion 10. The needle-shaped portion 10 projects from the housing portion 20 along the extending direction of the needle-shaped portion 10. The portion of the needle-shaped portion 10 protruding from the housing portion 20 includes at least a portion that enters the target region for transplantation in a living body.

The cell transplantation device 100 includes an inner member 30 inside the needle-shaped portion 10 and the housing portion 20. The inner member 30 includes a tubular portion 31 extending inside the needle-shaped portion 10 along the extending direction of the needle-shaped portion 10. The tubular portion 31 is an example of a rod-shaped portion. The tubular portion 31 is configured to be movable relative to the needle-shaped portion 10 along the extending direction of the needle-shaped portion 10.

The cell transplantation device 100 further includes a position changing portion 40 that changes the position of the tubular portion 31 with respect to the needle-shaped portion 10, a suction portion 50 that sucks the inside of the tubular portion 31, and a tubular portion 31 in the inner member 30. It is provided with a separation forming portion 60 for separating a portion including a tip portion of the above.

The position changing portion 40 includes a structure for moving the tubular portion 31 relative to the needle-shaped portion 10. The operation of the position changing unit 40 may be performed manually, or the position changing unit 40 may include an electronic component such as a motor, and the operation of the position changing unit 40 may be performed by electrical control.

The suction unit 50 includes a mechanism for sucking a fluid such as air by the movement of a pump or a piston. The operation of the suction unit 50 may be performed manually or by electrical control. The suction portion 50 may be directly or indirectly connected to the tubular portion 31 so that the inside of the tubular portion 31 can be sucked.

The separation forming portion 60 includes an edge portion 61 pressed against the tubular portion 31 and a moving mechanism portion 62 including a structure for moving the edge portion 61. The edge portion 61 is located outside the needle-shaped portion 10 and the housing portion 20. The operation of the moving mechanism unit 62 may be performed manually, or the moving mechanism unit 62 may include an electronic component such as a motor, and the operation of the moving mechanism unit 62 may be performed by electrical control.

As shown in FIG. 2, the cell transplantation device 100 may include a plurality of needle-shaped portions 10. The plurality of needle-shaped portions 10 are supported by one housing portion 20 and extend along one common direction. The arrangement of the plurality of needle-shaped portions 10 is not particularly limited, and the plurality of needle-shaped portions 10 may be arranged regularly or irregularly.

When the cell transplantation device 100 includes a plurality of needle-shaped portions 10, the inner member 30 includes a plurality of tubular portions 31, and one tubular portion 31 is arranged for each needle-shaped portion 10. That is, one cylindrical portion 31 is arranged inside one needle-shaped portion 10.

Hereinafter, in the drawings, a mode in which the cell transplantation device 100 includes a plurality of needle-shaped portions 10 will be illustrated.
A detailed configuration of the inner member 30 will be described with reference to FIG. The inner member 30 includes a number of tubular portions 31 corresponding to the number of needle-shaped portions 10. The outer shape of the tubular portion 31 is not particularly limited, and the tubular portion 31 may have a tubular shape extending along the extending direction of the needle-shaped portion 10. For example, when the needle-shaped portion 10 extends in a cylindrical shape, the tubular portion 31 may also be cylindrical. The tip surface of the tubular portion 31 is preferably a plane orthogonal to the extending direction of the tubular portion 31.

The tubular portion 31 has a separating portion 32 including the tip end portion of the tubular portion 31 and an extending portion 33 including the base end portion of the tubular portion 31. From the tip end portion of the tubular portion 31 toward the base end portion, the separation portion 32 and the extension portion 33 are arranged in this order, and the base end portion of the separation portion 32 and the tip end portion of the extension portion 33 are connected to each other. The extension portion 33 is an example of a support portion that supports the separation portion 32. The separation unit 32 is separated from the extension unit 33 when the implant is placed in the living body.

In the extending direction of the needle-shaped portion 10, the length of the tubular portion 31 is larger than the length of the needle-shaped portion 10. The length of the needle-shaped portion 10 is the length of the portion protruding from the housing portion 20.
The tubular portion 31 has a position where the tip of the tubular portion 31, that is, the tip of the separation portion 32 is arranged inside the needle-shaped portion 10, and the tip of the separation portion 32 is from the opening 11 of the needle-shaped portion 10. It is movable relative to the needle-shaped portion 10 with respect to the position of the needle-shaped portion 10 protruding to the outside. For example, the tubular portion 31 has a position where the tip portion of the separation portion 32 is arranged inside the needle-shaped portion 10 and a position where the entire separation portion 32 protrudes from the opening 11 to the outside of the needle-shaped portion 10. It is movable with respect to the needle-shaped portion 10 between them. The movement of the tubular portion 31 with respect to the needle-shaped portion 10 is performed based on the operation of the position changing portion 40 described above.

The material of the needle-shaped portion 10 and the housing portion 20 is not particularly limited. The material of the needle-shaped portion 10 and the housing portion 20 is, for example, metal or resin. The material of the extension portion 33 is also not particularly limited, and the material of the extension portion 33 is, for example, metal or resin. The separator 32 is formed from a biocompatible material. The separation portion 32 and the extension portion 33 may be formed of the same material or may be formed of different materials from each other. Further, the separated portion 32 and the extended portion 33 may be formed separately and then joined, or may be integrally formed. When the separation portion 32 and the extension portion 33 are integrally formed, it is preferable that a biocompatible resin is used as the material for the separation portion 32 and the extension portion 33. This makes it easy to configure the separation unit 32 so that it can be separated from the extension unit 33.

The tubular portion 31 may or may not have a clear boundary between the separating portion 32 and the extending portion 33. In other words, before the separation of the separation portion 32, the separation portion 32 and the extension portion 33 may or may not be distinguishable. If the tip of the tubular portion 31 can be separated due to breakage or the like, it can be said that the tubular portion 31 has the separating portion 32 and the extending portion 33.

When the cell transplantation device 100 includes a plurality of needle-shaped portions 10, the inner member 30 has, in addition to the plurality of tubular portions 31, one backbone portion 34 that supports the proximal ends of the plurality of tubular portions 31. Is preferable. The trunk portion 34 has a cylindrical shape and is located inside the housing portion 20. The space in each tubular portion 31 communicates with the space in the trunk portion 34.

When the inner member 30 includes the trunk portion 34, the position changing portion 40 may move the trunk portion 34 with respect to the housing portion 20 to move each cylindrical portion 31 collectively with respect to each needle-shaped portion 10. can. Therefore, the efficiency of movement of the tubular portion 31 with respect to the needle-shaped portion 10 is enhanced. Further, the suction portion 50 sucks the inside of the trunk portion 34, so that the inside of each tubular portion 31 is sucked together. Therefore, the efficiency of suction is improved.

The inner member 30 does not have to include the trunk portion 34. In this case, the plurality of tubular portions 31 may be moved separately with respect to the needle-shaped portions 10, or the inside of the plurality of tubular portions 31 may be separately sucked.

[How to use the cell transplant device]
A method of using the cell transplantation apparatus 100 will be described with reference to FIGS. 4 to 9. The method of using the cell transplant device 100 includes an uptake step, which is a step of taking the transplant into the cell transplant device 100, and a placement step, which is a step of arranging the transplant in a living body. The arranging step includes a separating step which is a step of separating the separating portion 32 at the tubular portion 31.

First, the import process will be described. As shown in FIG. 4, before transplantation, the transplanted product Cg is held in a tray 90 such as a culture container together with the protective liquid Pl. Protective solution Pl is a liquid that protects the transplanted Cg. For example, as shown in FIG. 4, the implant Cg and the protective solution Pl are placed in the recess 91 of the tray 90. The holding form of the transplant Cg and the protective liquid Pl in the tray 90 is not limited to the form in which the transplant Cg and the protective liquid Pl are held in the recess 91. For example, the transplant Cg and the protective liquid Pl may be arranged on the plane of the tray 90.

The protective solution Pl may have a composition that does not easily inhibit the survival of cells, and is preferably composed of a substance having a small effect on the living body when injected into the living body. For example, the protective liquid Pl is a liquid that protects the skin such as physiological saline, petrolatum or cosmetic water, or a mixture of these liquids. The protective liquid Pl may contain an additive component such as a nutritional component. Further, when the tray 90 is a culture container and the transplant Cg is cultured in the tray 90, the protective liquid Pl may be a medium for culturing cells, or may be a liquid exchanged from the medium. There may be. The protective liquid Pl can be a sol-like body, a gel-like body, a low-viscosity fluid, or a high-viscosity fluid.

In the uptake step, first, the cell transplantation device 100 is in a state in which the tip end portion of the separation portion 32 protrudes from the opening 11 of the needle-shaped portion 10 to the outside of the needle-shaped portion 10. A part of the separation portion 32 may come out from the opening 11, or the whole of the separation part 32 may come out. Then, the tip end portion of the separation portion 32 is brought close to the transplanted Cg arranged on the tray 90, and the inside of the tubular portion 31 is sucked.

As a result, as shown in FIG. 5, the transplant Cg is attracted to the tip of the separation portion 32, and the transplant Cg is held by the tubular portion 31 so as to abut on the tip of the separation portion 32. At this time, in a state where the protective liquid Pl is taken into the tubular portion 31 and the tubular portion 31 holds the transplanted Cg, at least a part of the tubular portion 31 may be filled with the protective liquid Pl. ..

Then, as shown in FIG. 6, the tubular portion 31 is moved with respect to the needle-shaped portion 10, and the tip portion of the separating portion 32 and the transplant Cg are drawn into the inside of the needle-shaped portion 10 from the opening 11. As a result, the transplanted Cg is taken into the inside of the needle-shaped portion 10. In the uptake step, the protective liquid Pl is taken into the needle-shaped portion 10 by suction in the needle-shaped portion 10, and the protective liquid Pl is present around the transplant Cg in the needle-shaped portion 10. May be good.

When the cell group containing the transplant Cg is a hair follicle primordium, one transplant Cg is incorporated into one needle-shaped portion 10. When the cell transplantation device 100 includes a plurality of needle-shaped portions 10, the arrangement of the plurality of needle-shaped portions 10 can control the distribution of hair formed based on the transplantation of the transplanted Cg.

When the cell group contained in the transplant Cg is a cell aggregate that contributes to hair growth or hair growth, such as a hair follicle primordium, the diameter of the cell group is, for example, 100 μm or more and 1000 μm or less. When the target of transplantation is such a cell aggregate, the needle-shaped portion 10 may be formed in a size capable of accommodating the transplanted product Cg containing the cell group having the above-mentioned size. Further, the inner diameter of the tip portion of the tubular portion 31, that is, the inner diameter of the tip portion of the separation portion 32 may be small enough to prevent the transplanted Cg from entering the inside of the tubular portion 31. The diameter of the cell group is the diameter of the sphere when the cell group is approximated to a sphere, in other words, the diameter of the smallest sphere circumscribing the cell group.

Next, the arrangement process will be described. After the uptake step, the cell transplantation device 100 is carried to the transplantation site Sk, which is, for example, the skin of the head of a living body. Then, as shown in FIG. 7, the transplantation site Sk is stabbed by the needle-shaped portion 10. At this time, the tip of the separation portion 32 and the transplant Cg are located inside the needle-shaped portion 10, and the tip of the needle-shaped portion 10 is located at the tip of the cell transplanting device 100. Since the tip of the needle-shaped portion 10 has a shape that easily sticks into the living body, the needle-shaped portion 10 can smoothly enter the transplantation site Sk. The needle-shaped portion 10 enters the transplantation site Sk until the tip of the needle-shaped portion 10 is arranged in the target area for transplantation.

Next, as shown in FIG. 8, the cylindrical portion 31 is moved relative to the needle-shaped portion 10, and the entire separated portion 32 is brought out from the opening 11. Specifically, the positions of the tubular portion 31 and the transplant Cg do not change, and the needle-shaped portion 10 is retracted in the direction of being pulled out from the transplant site Sk. As a result, since the transplanted Cg is placed in the space where the tip of the needle-shaped portion 10 was located inside the transplanted site Sk, the transplanted Cg is pressed from the surrounding tissue when it comes out of the needle-shaped portion 10. It is suppressed that the load is applied to the cells.

The needle-shaped portion 10 is retracted to a position where the entire needle-shaped portion 10 comes out of the transplantation site Sk. As a result, the tip of the separation portion 32 and the transplant Cg are located inside the transplant site Sk, and a part of the tubular portion 31 is exposed between the opening 11 of the needle-shaped portion 10 and the surface of the transplant site Sk. It will be in the state of being. At this time, the proximal end portion of the separation portion 32 protrudes from the surface of the transplantation site Sk. In other words, when there is a boundary between the separation portion 32 and the extension portion 33, the boundary is located between the opening 11 of the needle-shaped portion 10 and the surface of the transplantation site Sk.

For example, in a state where the transplanted product Cg is taken into the needle-shaped portion 10, the base end portion of the separating portion 32 is arranged inside the housing portion 20, so that the needle-shaped portion 10 becomes the transplanting site Sk. When stabbed, the base end portion of the separation portion 32 is arranged at a position where it does not enter the inside of the transplantation site Sk. Therefore, when the needle-shaped portion 10 is retracted, the proximal end portion of the separation portion 32 comes out from the surface of the transplantation site Sk.

Subsequently, a separation step is carried out. That is, as shown in FIG. 9, the separating portion 32 is separated from the extending portion 33. As a result, the transplant Cg is placed in the target region for transplantation at the transplant site Sk, and the separation portion 32 is placed in the vicinity of the transplant Cg. The separation portion 32 extends from the inside of the transplantation site Sk toward the surface and protrudes from the surface of the transplantation site Sk to the outside. The suction in the tubular portion 31 may be stopped after the needle-shaped portion 10 is pierced by the transplantation site Sk and before the separation portion 32 is separated. By stopping the suction, the holding of the transplanted Cg by the tubular portion 31 is released.

By arranging the separation portion 32 in the vicinity of the transplant Cg, it is possible to guide the direction of tissue formation based on the transplant of the transplant Cg. Specifically, tissue formation along the separation portion 32 is induced. Specifically, when the cell group contained in the transplant Cg is a hair follicle primordia, the separation portion 32 extends from the vicinity of the transplant Cg toward the skin surface, and the end portion of the separation portion 32 protrudes from the skin. As a result, hair follicles are formed up to the surface layer of the skin, and tissue formation can be induced so that the formed hairs go out of the skin. Therefore, it is possible to prevent the formed hair from being buried inside the skin.

In order to suitably promote the induction of tissue formation by the separating portion 32, the shortest distance between the separating portion 32 and the transplanted Cg is 100 μm in a state where the transplanted portion Cg and the separated portion 32 are arranged with respect to the living body. It is preferably within, and it is more preferable that the separation portion 32 is in contact with the transplanted Cg.

As described above, by arranging the separation portion 32, it is possible to form a surrounding environment capable of inducing tissue formation from the cell group. Then, according to the cell transplantation apparatus 100 of the present embodiment, since the separation portion 32 that induces tissue formation is arranged with respect to the living body together with the transplant Cg, the tissue formation is induced separately from the arrangement of the transplant Cg. Compared with arranging the members one by one at the transplantation site, the arrangement of the cell group in the living body and the formation of the surrounding environment can be easily and efficiently carried out. In particular, when the cell transplantation apparatus 100 includes a plurality of needle-shaped portions 10, the arrangement of the plurality of transplanted Cg and the arrangement of the plurality of separation portions 32 can be performed collectively, so that the arrangement of the cell group and the periphery thereof can be performed at the same time. It is more efficient with the formation of the environment.

Further, since the separation portion 32 includes the tip end portion of the tubular portion 31, the separation portion 32 has a function of holding the transplant Cg when taking in and transporting the transplant Cg, and also has a function of holding the transplant Cg in vivo. After the placement of, it functions as a member that induces tissue formation. Therefore, as compared with the case where the member for holding the transplanted Cg is provided separately from the separating portion 32, both the holding of the transplanted Cg and the formation of the surrounding environment of the cell group are suitably performed by a simple configuration. be able to.

After the tissue formation is induced, the separation part 32 may be removed, or if the separation part 32 is formed from a biodegradable material, the separation part 32 may not be removed.
[Separation process]
The details of the separation process and modification examples will be described with reference to FIGS. 10 to 13.

As shown in FIG. 10, in the separation step, the edge portion 61 of the separation forming portion 60 is laterally pushed into the tubular portion 31 exposed between the opening 11 of the needle-shaped portion 10 and the surface of the transplantation site Sk. You can guess. As a result, as a result of applying a force to the tubular portion 31 from a direction substantially orthogonal to the extending direction of the tubular portion 31, the tubular portion 31 is broken and the separated portion 32 is separated.

In the tubular portion 31, when a boundary exists between the separation portion 32 and the extension portion 33, the edge portion 61 is pressed against the boundary portion between the separation portion 32 and the extension portion 33. The position of the portion 61 is adjusted. In the tubular portion 31, when there is no boundary between the separating portion 32 and the extending portion 33, the edge portion 61 is pressed against an arbitrary position of the tubular portion 31 and the tubular portion 31 is broken. The portion separated by the above becomes the separation portion 32.

When the cell transplantation device 100 has a plurality of needle-shaped portions 10 and a plurality of tubular portions 31, the edge portion 61 sequentially arranges the plurality of tubular portions 31 along the direction in which the plurality of needle-shaped portions 10 are arranged. You may press it. For example, in the case of FIG. 10, the edge portion 61 moves from right to left toward the paper surface, and the separation portion 32 in each cylindrical portion 31 proceeds in order.

Alternatively, the edge portion 61 may press the plurality of tubular portions 31 together. For example, in the case of FIG. 10, the edge portion 61 moves from the front to the back of the paper surface, and the separation portions 32 in each cylindrical portion 31 are collectively separated.

The shape and material of the edge portion 61 may be selected according to the strength of the tubular portion 31. In short, it suffices if the tubular portion 31 can be broken by pressing the edge portion 61. Further, if the separation portion 32 can be separated by dividing the tubular portion 31, the edge portion 61 is not limited to the above-described configuration. For example, the edge portion 61 has a scissors shape and sandwiches the tubular portion 31. The separation unit 32 may be separated by cutting with.

As illustrated in FIG. 10, if the edge portion 61 is provided outside the needle-shaped portion 10 and the housing portion 20, the degree of freedom regarding the structure, size, movement, etc. of the edge portion 61 is large. Therefore, it is easy to configure the separation forming portion 60 including the edge portion 61 to be suitable for dividing the tubular portion 31, and the separation of the separation portion 32 can be smoothly promoted.

Hereinafter, a modification of the separation process will be described. In either case, the separation step is performed after the needle-shaped portion 10 is stabbed into the transplantation site Sk.
As shown in FIG. 11, the edge portion 61 may be located inside the housing portion 20, and the separation portion 32 may be separated inside the housing portion 20. In the case of FIG. 11, for example, the movement of the edge portion 61 from the front to the back of the paper surface breaks the tubular portion 31, but the movement of the edge portion 61 is not limited to this. Further, if the separation portion 32 can be separated by dividing the tubular portion 31, the edge portion 61 is not limited to the above-described configuration. For example, the edge portion 61 has a scissors shape and sandwiches the tubular portion 31. You may cut it with.

When the separation portion 32 is separated inside the housing portion 20, the separation step may be performed in a state where the needle-shaped portion 10 is pierced by the transplantation site Sk, or only the needle-shaped portion 10 is separated from the transplantation site Sk. It may be done after being pulled out. However, since the pressure in the tissue of the transplantation site Sk is higher than that in the needle-shaped portion 10, the separation portion 32 is separated in a state where the entire implant Cg and the separation portion 32 are located in the needle-shaped portion 10. When the needle-shaped portion 10 is pulled out, it is difficult for the implant Cg and the separating portion 32 to come out from the opening 11 of the needle-shaped portion 10 to the inside of the transplant site Sk. Therefore, it is preferable that the separation step is performed after the needle-shaped portion 10 is retracted to a position where the transplant Cg and at least a part of the separation portion 32 come out from the opening 11. If the implant Cg and at least a part of the separation portion 32 come out from the opening 11 to the inside of the transplantation site Sk, the surrounding tissue is entangled with the implant Cg and the end portion of the separation portion 32, so that the needle-shaped portion is formed. When the 10 is pulled out, the transplant Cg and the separation portion 32 tend to come out from the opening 11 and remain inside the transplant site Sk.

When the separating portion 32 is separated inside the housing portion 20, the tubular portion 31 does not have to be movable with respect to the needle-shaped portion 10 to the position where the entire separating portion 32 comes out from the opening 11. It suffices as long as it can move relative to the needle-shaped portion 10 to the position where each of the inserting step and the separating step is performed. In the separation step, as long as a part of the separation portion 32 protrudes from the opening 11, the tip of the separation portion 32 does not have to protrude to a position beyond the tip of the needle-shaped portion 10.

In the case where the separation portion 32 is separated inside the housing portion 20 without completely pulling out the needle-shaped portion 10 from the transplantation site Sk, the needle-shaped portion 10 is pulled out from the transplantation site Sk and then the needle-shaped portion is separated. The length required for the tubular portion 31 can be reduced as compared with the form in which the separation portion 32 is separated from the outside of the 10. Since the tubular portion 31 is a minute tubular structure, the shorter the tubular portion 31, the easier it is to form the tubular portion 31.

Further, the separation portion 32 may be separated by a method different from the division of the tubular portion 31 by the edge portion 61.
For example, as shown in FIG. 12, a force may be applied to the inner member 30 from the side inside the housing portion 20. That is, inside the housing portion 20, the tubular portion 31 and the trunk portion 34 are pushed or pulled in a direction substantially orthogonal to the extending direction of the tubular portion 31. As a result, the tubular portion 31 is broken near the boundary between the needle-shaped portion 10 and the housing portion 20, and the separating portion 32 is separated.

In this case, the separation forming portion 60 for separating the separating portion 32 includes a mechanism for applying a force in a direction substantially orthogonal to the extending direction of the tubular portion 31 to the inner member 30, and the cylinder is operated by the operation of the separation forming portion 60. A force is applied to the shape portion 31 and the trunk portion 34. Similar to the form shown in FIG. 11, the separation step may be performed in a state where the needle-shaped portion 10 is stuck in the transplantation site Sk, or is performed after only the needle-shaped portion 10 is pulled out from the transplantation site Sk. May be good. At this time, it is preferable that the separation step is performed after the needle-shaped portion 10 is retracted to a position where the transplant Cg and at least a part of the separation portion 32 come out from the opening 11.

According to the above configuration, the size of the housing portion 20 can be reduced as compared with the form in which the edge portion 61 is arranged in the vicinity of the cylindrical portion 31 in the housing portion 20. Further, since the separating portion 32 is separated by applying a force in one direction to the inner member 30, the structure of the separating forming portion 60 can be suppressed from becoming complicated.

Further, as shown in FIG. 13, the entire tubular portion 31 is a separation portion 32, and the inner member 30 includes a support portion that supports the base end portion of the separation portion 32 and supports the separation portion 32. It may be configured so that it can be released. For example, inside the housing portion 20, the trunk portion 34 supports the base end portion of the separation portion 32 in a releaseable manner, and the support of the base end portion is released, so that the separation portion 32 is separated. In the above configuration, the trunk portion 34 is an example of the support portion. Specifically, the base end portion of the separation portion 32 is passed through the hole formed in the trunk portion 34, and the support of the separation portion 32 is released by expanding this hole.

In this case, the separation forming portion 60 includes a mechanism for causing the support portion to release the support of the separation portion 32, and the support of the separation portion 32 is released by the operation of the separation forming portion 60. Similar to the form shown in FIG. 11, the separation step may be performed in a state where the needle-shaped portion 10 is stuck in the transplantation site Sk, or is performed after only the needle-shaped portion 10 is pulled out from the transplantation site Sk. May be good. At this time, it is preferable that the separation step is performed after the needle-shaped portion 10 is retracted to a position where the transplant Cg and at least a part of the separation portion 32 come out from the opening 11.

Even with the above configuration, the size of the housing portion 20 can be reduced as compared with the form in which the edge portion 61 is arranged in the vicinity of the cylindrical portion 31 in the housing portion 20. Further, since the length of the tubular portion 31 can be made shorter, the formation of the tubular portion 31 becomes easier.

When the separated portion 32 is separated by the breakage of the tubular portion 31, the tubular portion 31 has a fragile portion whose strength against an external force is lower than that of other portions of the tubular portion 31. Is preferable. The fragile portion includes the base end portion of the separation portion 32. The strength of the vulnerable portion to a force from a direction orthogonal to the extending direction of the tubular portion 31 is lower than, for example, the central portion of the extending portion 33 in the extending direction. Since the fragile portion is provided, when the tubular portion 31 receives an external force due to the operation of the separation forming portion 60, the tubular portion 31 is likely to be broken at the fragile portion. Therefore, the separation of the separation unit 32 proceeds smoothly.

FIG. 14 shows an example of a cylindrical portion 31 having a fragile portion. The fragile portion 35 includes the base end portion of the separation portion 32 and is located near the boundary between the separation portion 32 and the extension portion 33. In the fragile portion 35, the thickness of the side wall is smaller than that of the other portion of the tubular portion 31. In other words, in the fragile portion 35, the cross-sectional area along the direction orthogonal to the extending direction of the tubular portion 31 is smaller than that of the other portions of the tubular portion 31. The cross-sectional area is the area of the annular region where the side wall of the tubular portion 31 exists.

In order to preferably cause the tubular portion 31 to break at the fragile portion, the cross-sectional area of the fragile portion 35 is preferably 90% or less of the average cross-sectional area of the extended portion 33. The average cross-sectional area of the extension portion 33 is the average of the cross-sectional areas of the tip, the base end, and the midpoint between the tip and the base end of the extension portion 33.

By providing the fragile portion 35, as shown in FIG. 15, when the tubular portion 31 receives an external force due to the operation of the separation forming portion 60, the tubular portion 31 is broken by the fragile portion 35 and separated. The part 32 is separated. As a result, the separation of the separation portion 32 can be smoothly promoted, and the length of the separation portion 32 to be arranged with respect to the living body together with the transplanted Cg can be easily set in advance by setting the position of the fragile portion 35. Is.

Further, the fragile portion is not limited to a portion whose strength is reduced due to a difference in shape, such as a portion where the side wall of the tubular portion 31 is thinned, but is a portion whose strength is reduced due to a difference in material. There may be. For example, the fragile portion may be a portion having a lower strength than the other portions of the tubular portion 31, that is, a portion easily broken due to the difference in crystallinity. Specifically, the separated portion 32 and the extended portion 33 are integrally formed from the same resin material, and then heat is partially applied to change the crystallinity of the separated portion. The base end portion of 32 can be a fragile portion that is more easily broken than other portions. The entire separation portion 32 may be regarded as a fragile portion.

Further, when the separated portion 32 and the extended portion 33 are separately formed and joined by an adhesive or the like to form the tubular portion 31, the separated portion 32 and the extended portion 33 are joined. The strength of the portion tends to be lower than that of the other portions of the tubular portion 31. Taking advantage of this, the cylindrical portion 31 may be formed so that the strength of the joint portion is lower than that of the other portions, so that the joint portion may function as a fragile portion.

As described above, according to the first embodiment, the effects listed below can be obtained.
(1) The cell transplantation device 100 includes an inner member 30 having a separation portion 32 separable. Therefore, the cell transplantation device 100 can arrange the transplanted product Cg and the separation portion 32 with respect to the living body through the opening 11 of the needle-shaped portion 10. As a result, the separation portion 32 is arranged so as to extend from the inside of the living body toward the surface, so that tissue formation along the separation portion 32 is induced from the cell group. Therefore, it is possible to form a surrounding environment that can induce tissue formation from the cell group. Then, according to the cell transplantation apparatus 100, the separation portion 32 that induces tissue formation can be arranged with respect to the living body together with the transplanted product Cg, so that the arrangement of the cell group in the living body and the formation of the surrounding environment can be performed. It can be carried out easily and efficiently.

(2) The rod-shaped portion having the separating portion 32 is the tubular portion 31 which is a tubular structure. According to such a configuration, the separation portion 32 has a function of inducing tissue formation after placement in a living body and a function of holding the transplant Cg when the transplant Cg is taken into the needle-shaped portion 10. It can take on the function of assisting the transplantation of the transplanted Cg. Therefore, the transplantation of the transplanted product Cg can proceed smoothly.

(3) The tubular portion 31 is configured to be able to hold the transplanted Cg at the tip end portion of the tubular portion 31 by suction in the tubular portion 31. As a result, since the transplanted Cg can be stably held, the uptake of the transplanted Cg into the needle-shaped portion 10 and the transfer of the taken-in transplanted Cg can be smoothly promoted.

(4) Each of the separating portion 32 and the extending portion 33 which is a supporting portion supporting the base end portion of the separating portion 32 is a part of the rod-shaped portion, and the extending portion 33 extends to the base end portion of the separating portion 32. In the form in which the tip portions of the above are connected, it is possible to easily realize the configuration of the support portion that supports the separation portion 32 in a separable manner.

(5) If the rod-shaped portion has the fragile portion 35 at a position including the base end portion of the separation portion 32, the separation portion 32 is separated by applying an external force to the rod-shaped portion to break the fragile portion 35. be able to. Therefore, the separation unit 32 can be easily separated.

(6) If the cross-sectional area of the fragile portion 35 is 90% or less of the average cross-sectional area of the extension portion 33 which is the support portion, the fragile portion 35 is likely to break due to an external force, so that the separation portion 32 can be separated smoothly. Can be done.

(7) When the whole rod-shaped portion is the separated portion 32, the length of the rod-shaped portion can be shortened as compared with the form in which a part of the rod-shaped portion is the separated portion 32. Therefore, it becomes easy to form a minute rod-shaped portion.

(8) The separation forming portion 60 separates the separation portion 32 in a state where the entire separation portion 32 protrudes from the opening 11 of the needle-shaped portion 10. According to such a configuration, a mechanism for separating the separating portion 32 is arranged outside the structure having the needle-shaped portion 10. Therefore, the degree of freedom regarding the structure, size, movement, etc. of the mechanism is large. Therefore, it is easy to configure the mechanism suitable for dividing the rod-shaped portion, and the separation of the separation portion 32 can be smoothly promoted.

(9) The separation forming portion 60 separates the separation portion 32 in a state where the base end portion of the separation portion 32 does not protrude from the opening 11 of the needle-shaped portion 10. According to such a configuration, it is possible to shorten the length of the entire rod-shaped portion as compared with the form in which the separated portion 32 is separated with the entire separated portion 32 protruding from the opening 11 of the needle-shaped portion 10. be. Therefore, it becomes easy to form a minute rod-shaped portion.

(10) If the needle-shaped portion 10 is configured to be capable of accommodating a transplanted product Cg containing a cell aggregate having a diameter of 100 μm or more and 1000 μm or less, the cell transplanting apparatus 100 may grow hair such as a hair follicle primordium or the like. It is a device suitable for transplanting cell groups that contribute to hair growth.

(11) The cell transplantation device 100 is used for transplanting a transplanted product Cg containing a cell aggregate containing skin-derived stem cells into a living body. According to such a configuration, by using the cell transplantation device 100, the separation portion 32 is arranged with respect to the skin of the living body together with the cell group that contributes to hair growth or hair growth such as the hair follicle primordium. Then, the separation portion 32 extends from the vicinity of the cell group toward the skin surface, and the end portion of the separation portion 32 protrudes from the skin, so that hair follicles are formed up to the skin surface layer, and the formed hairs are outside the skin. Histogenesis can be induced to go out to.

(12) The method of using the cell transplantation device 100 is to take the transplanted product Cg into the needle-shaped portion 10, pierce the needle-shaped portion 10 into a living body, and then attach the separated portion 32 and the transplanted product Cg to the needle-shaped portion. It includes taking out from the opening 11 of 10 and separating the separating portion 32 from the supporting portion. According to such a method, the transplant Cg and the separation portion 32 are arranged with respect to the living body through the opening 11 of the needle-shaped portion 10. In this way, since the separation portion 32 that induces tissue formation can be arranged with respect to the living body together with the transplanted Cg, the arrangement of the cell group in the living body and the formation of the surrounding environment can be easily and efficiently performed. Can be carried out.

(Second Embodiment)
A second embodiment of the cell transplantation apparatus and its usage will be described with reference to FIGS. 16 to 19. The cell transplantation apparatus of the second embodiment is also used for transplantation of the same transplant as the first embodiment. The second embodiment has a different structure of the inner member as compared with the first embodiment. Hereinafter, the differences between the second embodiment and the first embodiment will be mainly described, and the same reference numerals will be given to the same configurations as those of the first embodiment, and the description thereof will be omitted.

[Cell transplant device]
As shown in FIG. 16, the inner member 70 included in the cell transplantation device 110 of the second embodiment includes a columnar portion 71 instead of the tubular portion 31. The columnar portion 71 is an example of a rod-shaped portion. The columnar portion 71 is a structure having no space for fluid to flow inside. The columnar portion 71 extends inside the needle-shaped portion 10 along the extending direction of the needle-shaped portion 10. In the extending direction of the needle-shaped portion 10, the length of the columnar portion 71 is larger than the length of the needle-shaped portion 10. The outer shape of the columnar portion 71 is not particularly limited. For example, when the needle-shaped portion 10 extends in a cylindrical shape, the columnar portion 71 may be cylindrical. The tip surface of the columnar portion 71 is preferably a plane orthogonal to the extending direction of the columnar portion 71.

The columnar portion 71 has a separating portion 72 including the tip end portion of the columnar portion 71, and an extending portion 73 including the base end portion of the columnar portion 71. The separation portion 72 and the extension portion 73 are arranged in this order from the tip end portion of the columnar portion 71 toward the base end portion, and the base end portion of the separation portion 72 and the tip end portion of the extension portion 73 are connected to each other. The extension portion 73 is an example of a support portion that supports the separation portion 72. The separation portion 72 is separated from the extension portion 73 when the implant is placed in the living body.

The material of the extension portion 73 is not particularly limited, and the material of the extension portion 73 is, for example, metal or resin. The separator 72 is formed from a biocompatible material. The separation portion 72 and the extension portion 73 may be formed of the same material or may be formed of different materials from each other. Further, the separated portion 72 and the extended portion 73 may be formed separately and then joined, or may be integrally formed. When the separation portion 72 and the extension portion 73 are integrally formed, it is preferable that a biocompatible resin is used as the material for the separation portion 72 and the extension portion 73. As a result, it is easy to configure the separating portion 72 so as to be separable from the extending portion 73.

The columnar portion 71 may or may not have a clear boundary between the separated portion 72 and the extended portion 73. In other words, before the separation portion 72 is separated, the separation portion 72 and the extension portion 73 may or may not be distinguishable. If the tip of the columnar portion 71 can be separated due to breakage or the like, it can be said that the columnar portion 71 has the separated portion 72 and the extended portion 73.

The columnar portion 71 has a position where the tip portion of the columnar portion 71, that is, the tip portion of the separation portion 72 is arranged inside the needle-shaped portion 10, and the tip portion of the separation portion 72 is needle-shaped from the opening 11 of the needle-shaped portion 10. It is configured to be relatively movable with respect to the needle-shaped portion 10 from the position protruding to the outside of the portion 10. The movement of the columnar portion 71 with respect to the needle-shaped portion 10 is performed based on the operation of the position changing portion 40. In the second embodiment, the position changing portion 40 includes a structure for moving the columnar portion 71 relative to the needle-shaped portion 10. Further, in the second embodiment, the suction portion 50 sucks the inside of the needle-shaped portion 10.

Although not shown, the cell transplantation device 110 of the second embodiment also has a separation forming portion 60 including a mechanism for separating the separating portion 72 of the columnar portion 71 in the inner member 70, as in the first embodiment. I have.

When the cell transplantation device 110 includes a plurality of needle-shaped portions 10, the inner member 70 includes a plurality of columnar portions 71, and one columnar portion 71 is arranged for each needle-shaped portion 10. At this time, it is preferable that the inner member 70 has one trunk portion 74 that supports the base end portions of the plurality of columnar portions 71. The trunk portion 74 is located inside the housing portion 20. By moving the trunk portion 74 with respect to the housing portion 20, the position changing portion 40 can move each columnar portion 71 collectively with respect to each needle-shaped portion 10. Therefore, the efficiency of movement of the columnar portion 71 with respect to the needle-shaped portion 10 is enhanced.

The inner member 70 does not have to include the trunk portion 74. In this case, the plurality of columnar portions 71 may be moved separately with respect to the needle-shaped portion 10. Further, regardless of the presence or absence of the trunk portion 74, the suction portion 50 may suck the inside of the plurality of needle-shaped portions 10 together by sucking the inside of the housing portion 20, or the suction portion 50 may have a plurality of needle-shaped portions. The inside of the portion 10 may be sucked separately.

[How to use the cell transplant device]
Also in the second embodiment, the method of using the cell transplant device 110 includes a step of taking in the transplant and a step of arranging, and the arranging step includes a step of separating the separation unit 72.

In the taking-in step of the second embodiment, the tip of the needle-shaped portion 12 is placed in the needle-shaped portion 10, and the tip of the needle-shaped portion 10 is the same as that of the first embodiment. It is brought close to the implant Cg arranged on the tray, and the inside of the needle-shaped portion 10 is sucked.

As a result, as shown in FIG. 17, the transplant Cg is drawn into the needle-shaped portion 10 from the opening 11. Inside the needle-shaped portion 10, a space in which the transplant Cg is fastened is partitioned between the tip of the columnar portion 71 and the opening 11, and the transplant Cg is housed in this space. The gap between the side surface of the columnar portion 71 and the inner surface of the needle-shaped portion 10 is narrow enough to prevent the implant Cg from entering, and the implant Cg is fastened between the columnar portion 71 and the opening 11.

When the transplant Cg is taken into the needle-shaped portion 10, the protective liquid held together with the transplant Cg in the tray is also taken into the needle-shaped portion 10 and around the transplant Cg inside the needle-shaped portion 10. May have a protective solution.

The tip of the columnar portion 71 may have adhesiveness. If the tip of the columnar portion 71 has adhesiveness, the transplanted Cg adheres to the tip of the columnar portion 71, so that the transplanted Cg is held at the tip of the columnar portion 71. As a result, the transplanted Cg can be stably held in the needle-shaped portion 10.

Further, when the tip portion of the columnar portion 71 has adhesiveness, the tip portion of the columnar portion 71, that is, the tip portion of the separation portion 72 is from the opening 11 of the needle-shaped portion 10 as in the first embodiment. In the protruding state, the implant Cg in the tray may be held at the tip of the columnar portion 71. After that, the columnar portion 71 is moved with respect to the needle-shaped portion 10, so that the transplanted Cg is taken into the inside of the needle-shaped portion 10.

In addition to the columnar portion 71, the cell transplanting device 110 includes, for example, a filter or a net-like structure as a configuration for retaining the transplanted Cg in the vicinity of the tip portion of the needle-shaped portion 10 inside the needle-shaped portion 10. May be.

The placement step and the separation step are performed in the same manner as in the first embodiment. That is, after the uptake step, the cell transplantation device 110 is carried to the transplantation site Sk, and the transplantation site Sk is stabbed by the needle-shaped portion 10. Then, as shown in FIG. 18, only the needle-shaped portion 10 is retracted and pulled out from the transplantation site Sk, so that the entire separation portion 72 is brought out from the opening 11, and then the separation portion 72 is separated. Will be done.

When the needle-shaped portion 10 is pulled out, the columnar portion 71 is located on the surface side of the transplantation site Sk with respect to the transplanted product Cg, so that the transplanted product Cg is suppressed from moving together with the needle-shaped portion 10, and the transplanted product Cg becomes It is left inside the transplant site Sk.

In the separation step, as in the first embodiment, the edge portion 61 of the separation forming portion 60 is laterally pressed against the columnar portion 71 exposed between the opening 11 of the needle-shaped portion 10 and the surface of the transplantation site Sk. Be done. As a result, the columnar portion 71 is broken and the separation portion 72 is separated.

As a result, the transplant Cg is placed in the target region for transplantation at the transplant site Sk, and the separation portion 72 is placed in the vicinity of the transplant Cg. The separation portion 72 extends from the inside of the transplantation site Sk toward the surface and protrudes from the surface of the transplantation site Sk to the outside.

By arranging the separation portion 72 in the vicinity of the transplanted product Cg, it is possible to form a surrounding environment capable of inducing tissue formation from the cell group. Also in the cell transplantation device 110 of the second embodiment, the separation portion 72 that induces tissue formation is arranged with respect to the living body together with the transplanted Cg, so that the arrangement of the cell group in the living body and the formation of the surrounding environment can be performed. It can be carried out efficiently.

In order to suitably promote the induction of tissue formation by the separating portion 72, the separating portion 72 and the transplanted product Cg are provided in a state where the transplanted portion Cg and the separated portion 72 are arranged with respect to the living body, as in the first embodiment. The shortest distance between them is preferably within 100 μm, and it is more preferable that the separation portion 72 is in contact with the transplanted Cg.

In the second embodiment, the separation portion 72 is not a cylinder but a columnar shape, and has a shape more suitable for inducing tissue formation. In particular, when the separation portion 72 does not have the function of holding the transplanted Cg, the separation portion 72 can be formed into a thickness, length, shape, material, surface state, etc. more suitable for inducing tissue formation. .. Therefore, it is possible to more accurately form the surrounding environment of the cell group in the living body.

Any of the modified examples of the separation step described in the first embodiment can be applied to the second embodiment. That is, the edge portion 61 of the separation forming portion 60 may be pressed against the columnar portion 71 from the side inside the housing portion 20, so that the columnar portion 71 may be broken and the separation portion 72 may be separated.

Alternatively, when a force is applied to the inner member 70 from the side inside the housing portion 20, the columnar portion 71 is broken near the boundary between the needle-shaped portion 10 and the housing portion 20, and the separation portion 72 is formed. It may be separated.

Alternatively, the entire columnar portion 71 is a separation portion 72, and the inner member 70 includes a support portion that supports the base end portion of the separation portion 72, and is configured so that the support of the separation portion 72 can be released. May be good. The separation portion 72 is separated by releasing the support of the base end portion by the support portion.

Further, also in the second embodiment, when the separation portion 72 is separated by the breakage of the columnar portion 71, it is preferable that the columnar portion 71 has a fragile portion in a portion including the base end portion of the separation portion 72. .. The strength of the vulnerable portion to a force from a direction orthogonal to the extending direction of the columnar portion 71 is lower than, for example, the central portion of the extending portion 73 in the extending direction.

FIG. 19 shows an example of a columnar portion 71 having a fragile portion. The fragile portion 75 includes the base end portion of the separation portion 72, and is located near the boundary between the separation portion 72 and the extension portion 73. In the fragile portion 75, the columnar portion 71 is thin. That is, in the fragile portion 75, the cross-sectional area along the direction orthogonal to the extending direction of the columnar portion 71 is smaller than that of the other portions of the columnar portion 71. In order to preferably cause the columnar portion 71 to break at the fragile portion 75, the cross-sectional area of the fragile portion 75 is preferably 90% or less of the average cross-sectional area of the extended portion 73. The average cross-sectional area of the extension portion 73 is the average of the cross-sectional areas of the tip, the base end, and the midpoint between the tip and the base end of the extension portion 73.

By providing the fragile portion 75, when the columnar portion 71 receives an external force due to the operation of the separation forming portion 60, the columnar portion 71 is broken at the fragile portion 75 and the separation portion 72 is separated. As a result, the separation of the separation portion 72 can be smoothly promoted, and the length of the separation portion 72 to be arranged with respect to the living body together with the transplanted Cg can be easily set in advance by setting the position of the fragile portion 75. Is.

As in the first embodiment, the fragile portion is not limited to the portion whose strength is lowered due to the difference in shape, but may be the portion whose strength is lowered due to the difference in material. That is, the fragile portion may be a portion that is more easily broken than the other portion of the columnar portion 71 due to the difference in crystallinity, and the strength of the joint portion between the separated portion 72 and the extended portion 73 is the other portion. By forming the columnar portion 71 so as to be lower than the above-mentioned joint portion, the joint portion may function as a fragile portion.

As described above, according to the second embodiment, the following effects can be obtained in addition to the effects of (1), (4) to (12) of the first embodiment.
(13) The rod-shaped portion having the separating portion 72 is a columnar portion 71 which is a columnar structure having no fluid flow path inside. According to such a configuration, the rod-shaped portion has a shape more suitable for inducing histogenesis than the case where the rod-shaped portion has a cylindrical shape. Therefore, the surrounding environment of the cell group can be more preferably formed.

(Third Embodiment)
A third embodiment of the cell transplantation apparatus and its usage will be described with reference to FIGS. 20 to 23. The cell transplantation apparatus of the third embodiment is also used for transplantation of the same transplant as the first embodiment. The third embodiment has a different structure of the inner member as compared with the first embodiment and the second embodiment. Hereinafter, the differences between the third embodiment, the first embodiment, and the second embodiment will be mainly described, and the same configurations as those of the first embodiment and the second embodiment will be described with the same reference numerals. Is omitted.

[Cell transplant device]
As shown in FIG. 20, the inner member 80 included in the cell transplantation apparatus 120 of the third embodiment includes a cylindrical portion 81 and a columnar portion 82. The columnar portion 82 is an example of a rod-shaped portion. Each of the tubular portion 81 and the columnar portion 82 extends in parallel inside the needle-shaped portion 10 along the extending direction of the needle-shaped portion 10. The tubular portion 81 is a tubular structure having a space through which the fluid flows, and the columnar portion 82 is a structure having no space in which the fluid flows inside. In the extending direction of the needle-shaped portion 10, the length of the columnar portion 82 is larger than the length of the needle-shaped portion 10.

The outer shapes of the tubular portion 81 and the columnar portion 82 are not particularly limited. For example, the tubular portion 81 is cylindrical, and the columnar portion 82 is cylindrical. The tip surfaces of the tubular portion 81 and the columnar portion 82 are preferably planes orthogonal to the extending direction of the needle-shaped portion 10.

The columnar portion 82 has the same configuration as that of the second embodiment and the columnar portion 71. That is, the columnar portion 82 has a separating portion 83 and an extending portion 84. The separation portion 83 includes the tip end portion of the columnar portion 82, and the base end portion of the separation portion 83 and the tip end portion of the extension portion 84 are connected to each other. The extension portion 84 is an example of a support portion.

The tubular portion 81 does not have a separation portion that is separated when the implant is placed. In the extending direction of the needle-shaped portion 10, the position of the tip of the tubular portion 81 and the position of the tip of the columnar portion 82 may be the same or different. In order to arrange the separation portion 83 closer to the implant, it is preferable that the tip portion of the columnar portion 82 extends beyond the tip portion of the tubular portion 81 in the extending direction of the needle-shaped portion 10. ..

The tubular portion 81 and the columnar portion 82 may be formed of the same material or may be formed of different materials. Further, the cylindrical portion 81 and the columnar portion 82 may be members formed separately, or are integrally formed so as to be connected at the base end portion of the tubular portion 81 and the columnar portion 82. You may.

The tubular portion 81 and the columnar portion 82 have a position where their tip portions are arranged inside the needle-shaped portion 10 and a position where the tip portion projects from the opening 11 of the needle-shaped portion 10 to the outside of the needle-shaped portion 10. It is configured to be relatively movable with respect to the needle-shaped portion 10. The tubular portion 81 and the columnar portion 82 are collectively moved with respect to the needle-shaped portion 10.

The movement of the cylindrical portion 81 and the columnar portion 82 with respect to the needle-shaped portion 10 is performed based on the operation of the position changing portion 40. In the third embodiment, the position changing portion 40 includes a structure for moving the cylindrical portion 81 and the columnar portion 82 relative to the needle-shaped portion 10. Further, in the second embodiment, the suction portion 50 sucks the inside of the tubular portion 81.

Although not shown, the cell transplantation device 120 of the third embodiment also has a separation forming portion 60 including a mechanism for separating the separating portion 83 of the columnar portion 82 in the inner member 80, as in the first embodiment. I have.

When the cell transplantation device 120 includes a plurality of needle-shaped portions 10, the inner member 80 includes a plurality of tubular portions 81 and a plurality of columnar portions 82, and each needle-shaped portion 10 includes a tubular portion 81 and a columnar portion 82. Are arranged one by one. At this time, it is preferable that the inner member 80 has one trunk portion 85 that supports the base end portions of the plurality of tubular portions 81 and the proximal end portions of the plurality of columnar portions 82. The trunk portion 85 is located inside the housing portion 20. By moving the trunk portion 85 with respect to the housing portion 20, the position changing portion 40 can move each cylindrical portion 81 and each columnar portion 82 collectively with respect to each needle-shaped portion 10. Therefore, the efficiency of movement of the cylindrical portion 81 and the columnar portion 82 with respect to the needle-shaped portion 10 is enhanced. Further, the suction portion 50 sucks the inside of the trunk portion 85, so that the inside of each tubular portion 81 is sucked together. Therefore, the efficiency of suction is improved.

The inner member 80 does not have to include the trunk portion 85. In this case, the tubular portion 81 and the columnar portion 82 may be moved with respect to the needle-shaped portion 10 separately for each needle-shaped portion 10. Further, the suction unit 50 may separately suck the inside of the plurality of tubular portions 81.

[How to use the cell transplant device]
Also in the third embodiment, the method of using the cell transplantation apparatus 120 includes a step of taking in the transplant and a step of arranging, and the arranging step includes a step of separating the separation unit 83.

In the loading step of the third embodiment, as in the first embodiment, the tip of the tubular portion 81 is arranged on a tray such as a culture container in a state of protruding outward from the opening 11 of the needle-shaped portion 10. The tip of the tubular portion 81 is brought close to the Cg of the transplanted product, and the inside of the tubular portion 81 is sucked. As a result, the transplant Cg is held at the tip of the tubular portion 81. Then, the tubular portion 81 is moved with respect to the needle-shaped portion 10, and the tip portion of the tubular portion 81 and the transplant Cg are drawn into the inside of the needle-shaped portion 10 from the opening 11. As a result, as shown in FIG. 21, the transplanted Cg is taken into the inside of the needle-shaped portion 10.

In the placement step, the cell transplant device 120 is carried to the transplant site Sk, and the transplant site Sk is stabbed by the needle-shaped portion 10. Then, only the needle-shaped portion 10 is retracted and pulled out from the transplantation site Sk, so that the entire separation portion 83 is brought out from the opening 11. After that, as shown in FIG. 22, the separation unit 83 is separated. Further, when the suction by the suction portion 50 is stopped, the holding of the transplanted Cg by the tubular portion 81 is released.

In the separation step, as in the first embodiment, the edge portion 61 of the separation forming portion 60 is laterally pressed against the columnar portion 82 exposed between the opening 11 of the needle-shaped portion 10 and the surface of the transplantation site Sk. By doing so, the columnar portion 82 is broken and the separation portion 83 is separated. The edge portion 61 may be pressed against the columnar portion 82 from a direction that is not obstructed by the tubular portion 81, and the plurality of columnar portions 82 may be sequentially broken by the appropriate movement of one edge portion 61. It may be broken separately by a plurality of edge portions 61.

As a result, the transplant Cg is placed in the target region for transplantation at the transplant site Sk, and the separation portion 83 is placed in the vicinity of the transplant Cg. Also in the cell transplantation device 120 of the third embodiment, the separation portion 83 that induces tissue formation is arranged with respect to the living body together with the transplanted Cg, so that the arrangement of the cell group in the living body and the formation of the surrounding environment can be performed. It can be carried out efficiently.

In order to suitably promote the induction of tissue formation by the separating portion 83, as in the first embodiment, in the state where the transplant Cg and the separating portion 83 are arranged with respect to the living body, the separating portion 83 and the transplant Cg are used. The shortest distance between them is preferably within 100 μm.

In the third embodiment, a tubular portion 81 which is a member for holding the transplanted Cg is provided separately from the separating portion 83 which functions as a member for inducing tissue formation. Therefore, the separated portion 83 and the tubular portion 81 can be formed to have a thickness, length, shape, material, surface condition, etc. that are more suitable for each function. Therefore, the retention of the transplanted Cg and the formation of the surrounding environment of the cell group are accurately performed.

Any modification of the separation step described in the first embodiment can be applied to the third embodiment. That is, the edge portion 61 of the separation forming portion 60 may be pressed against the columnar portion 82 from the side inside the housing portion 20, so that the columnar portion 82 may be broken and the separation portion 83 may be separated.

Alternatively, when a force is applied to the inner member 80 from the side inside the housing portion 20, the columnar portion 82 is broken near the boundary between the needle-shaped portion 10 and the housing portion 20, and the separation portion 83 is formed. It may be separated.

Alternatively, the entire columnar portion 82 is a separation portion 83, and the inner member 80 includes a support portion that supports the base end portion of the separation portion 83, and is configured so that the support of the separation portion 83 can be released. May be good. The separation portion 83 is separated by releasing the support of the base end portion by the support portion.

Further, also in the third embodiment, when the separation portion 83 is separated by the breakage of the columnar portion 82, it is preferable that the columnar portion 82 has a fragile portion in a portion including the base end portion of the separation portion 83. .. The strength of the vulnerable portion to a force from a direction orthogonal to the extending direction of the columnar portion 82 is lower than, for example, the central portion of the extending portion 84 in the extending direction.

FIG. 23 shows an example of a columnar portion 82 having a fragile portion. The fragile portion 86 includes the base end portion of the separation portion 83, and is located near the boundary between the separation portion 83 and the extension portion 84. In the fragile portion 86, the columnar portion 82 is thin. That is, in the fragile portion 86, the cross-sectional area along the direction orthogonal to the extending direction of the columnar portion 82 is smaller than that of the other portions of the columnar portion 82. In order to preferably cause the columnar portion 82 to break at the fragile portion 86, the cross-sectional area of the fragile portion 86 is preferably 90% or less of the average cross-sectional area of the extended portion 84. The average cross-sectional area of the extension portion 84 is the average of the cross-sectional areas of the tip, the base end, and the midpoint between the tip and the base end of the extension portion 84.

As in the first embodiment, the fragile portion is not limited to the portion whose strength is lowered due to the difference in shape, but may be the portion whose strength is lowered due to the difference in material. That is, the fragile portion may be a portion that is more easily broken than the other portion of the columnar portion 82 due to the difference in crystallinity. Further, by forming the columnar portion 82 so that the strength of the joint portion between the separation portion 83 and the extension portion 84 is lower than that of the other portions, the joint portion may function as a fragile portion.

In the above embodiment, the embodiment in which the tubular portion 81 and the columnar portion 82 are collectively moved with respect to the needle-shaped portion 10 has been described, but the tubular portion 81 and the columnar portion 82 are respectively relative to the needle-shaped portion 10. It may be moved separately. For example, in the taking-in step, the tip of the tubular portion 81 is in a state of protruding from the opening 11 of the needle-shaped portion 10, while the tip of the columnar portion 82 is always arranged inside the needle-shaped portion 10. May be done. Further, when the columnar portion 82 is divided between the opening 11 of the needle-shaped portion 10 and the surface of the transplantation site Sk in the separation step, the tubular portion 81 released from holding the transplanted Cg is inside the needle-shaped portion 10. After being drawn into, the separation unit 83 may be separated.

As described above, according to the third embodiment, the following effects can be obtained in addition to the effects of (1), (3) to (12) of the first embodiment.
(14) The rod-shaped portion having the separating portion 83 is a columnar portion 82 which is a columnar structure having no fluid flow path inside, and the inner member 80 has a tubular structure extending in parallel with the columnar portion 82. It has a tubular portion 81 which is a body. According to such a configuration, the columnar portion 82, which is a rod-shaped portion, has a shape more suitable for inducing tissue formation than the case where the columnar portion 82 is cylindrical. Further, the tubular portion 81 can be used to hold the transplanted product Cg when the transplanted product Cg is taken into the needle-shaped portion 10. Since the functions of the columnar portion 82 and the tubular portion 81 are separated, the columnar portion 82 and the tubular portion 81 can be configured to be more suitable for each function. Retention and formation of the surrounding environment of the cell group can be performed accurately.

Cg ... Implant 10 ... Needle-shaped part 11 ... Opening 20 ... Housing part 30, 70, 80 ... Inner member 31, 81 ... Cylindrical part 71, 82 ... Rod-shaped part 32, 72, 83 ... Separation part 33, 73, 84 ... Extension part 34,74,85 ... Core part 35,75,86 ... Vulnerable part 40 ... Position change part 50 ... Suction part 60 ... Separation formation part 61 ... Edge part 90 ... Tray 100,110,120 ... Cell transplantation Device

Claims (14)

The needle that extends in a cylindrical shape and has an opening at the tip of the needle-shaped portion so that a transplant containing a cell group to be transplanted into a living body can be accommodated inside. The shape and the shape
An inner member comprising a rod-shaped portion extending inward of the needle-shaped portion.
The rod-shaped portion has the needle shape between a position where the tip portion of the rod-shaped portion is arranged inside the needle-shaped portion and a position where the tip portion of the rod-shaped portion protrudes from the opening of the needle-shaped portion. It is configured to be movable relative to the part,
The inner member has a separating portion including the tip end portion of the rod-shaped portion and a supporting portion that supports the base end portion of the separating portion, and the separating portion is configured to be separable from the supporting portion. Cell transplant device. The cell transplantation apparatus according to claim 1, wherein the rod-shaped portion is a tubular structure. The cell transplantation apparatus according to claim 1, wherein the rod-shaped portion is a columnar structure having no fluid flow path inside. The rod-shaped portion is a columnar structure having no fluid flow path inside, and is a columnar structure.
The cell transplanting apparatus according to claim 1, wherein the inner member includes a tubular portion which is a tubular structure extending in parallel with the rod-shaped portion inside the needle-shaped portion. The tubular portion, which is the tubular structure, is connected to a suction portion that sucks the inside of the tubular portion.
The cell transplantation apparatus according to claim 2 or 4, wherein the tubular portion is configured to be able to hold the transplant at the tip end portion of the tubular portion by suction in the tubular portion. Each of the separation portion and the support portion is a part of the rod-shaped portion.
The cell transplantation apparatus according to any one of claims 1 to 5, wherein the tip end portion of the support portion is connected to the base end portion of the separation portion. The cell transplanting apparatus according to claim 6, wherein the rod-shaped portion has a fragile portion at a position including a base end portion of the separating portion, which is a portion having a lower strength against an external force than other portions of the rod-shaped portion. The cell transplantation apparatus according to claim 7, wherein the cross-sectional area of the fragile portion is 90% or less of the average cross-sectional area of the support portion. The cell transplantation apparatus according to any one of claims 1 to 5, wherein the entire rod-shaped portion is the separation portion. A separation forming portion including a mechanism for separating the separation portion from the support portion is provided.
The invention according to any one of claims 1 to 9, wherein the separation forming portion is configured to separate the separation portion in a state where the entire separation portion protrudes from the opening of the needle-shaped portion. Cell transplant device. A separation forming portion including a mechanism for separating the separation portion from the support portion is provided.
The separation forming portion is according to any one of claims 1 to 9, wherein the separation forming portion is configured to separate the separation portion in a state where the base end portion of the separation portion does not protrude from the opening of the needle-shaped portion. The cell transplant device described. The cell according to any one of claims 1 to 11, wherein the needle-shaped portion is configured to accommodate the transplant containing the cell group, which is a cell aggregate having a diameter of 100 μm or more and 1000 μm or less. Transplant device. The cell transplantation apparatus according to any one of claims 1 to 12, which is used for transplanting the transplant containing the cell group, which is a cell aggregate containing skin-derived stem cells, into a living body. The method for using the cell transplantation apparatus according to any one of claims 1 to 13.
Incorporating the implant into the needle-like portion and
After the needle-shaped portion is pierced into a living body, the separated portion and the transplanted portion are taken out from the opening of the needle-shaped portion.
Separating the separation part from the support part and
How to use a cell transplantation device, including.

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