JP2022074206A - Fluorescence detection optical device and method of using fluorescence detection optical device - Google Patents

Abstract

To provide a fluorescence detection optical device using LEDs as light source, capable of solving a problem that as a light emission point of LED has large area, in a case where a small fluorescence detection optical system is configured, a component is generated which causes excitation light to enter an emission filter at a small angle, thereby the excitation light entering a photodetector.SOLUTION: A light blocking plate having a plane including fluorescent light optical axis is interposed between a photodetector and a fluorescence reagent so that light entering an emission filter at a small angle generated by using LEDs as light source is blocked, to thereby prevent blocking properties of the emission filter from deteriorating.SELECTED DRAWING: Figure 11

Description

It is important to prevent the spread of viruses, because the spread of viruses that humans do not have immunity will have a serious impact on society. Prompt isolation of infected people or animals is important as a means of stopping the spread of the virus.
In order to quickly isolate a person or animal infected with a virus, it is necessary to easily and accurately determine whether or not the person or animal is infected with the virus. In order to easily and accurately determine whether or not a virus is infected, it is necessary to increase the number of diagnostic devices that are infected with a virus, and the diagnostic devices are becoming smaller and cheaper. It has been demanded.
As a method for diagnosing whether or not a virus is infected, a method of amplifying a gene using a PCR method (polymerase chain reaction) and then diagnosing with a fluorescence microscope device is generally used. However, since the fluorescence optical system of the conventional fluorescence microscope device is expensive and large, it is desired to reduce the size and price of the fluorescence optical system in order to reduce the size and price of the diagnostic device. Therefore, the present invention relates to a compact and low-priced fluorescence detection optical device capable of detecting an amplified gene and a method of using the same.

FIG. 1 shows an optical system configuration of a conventional fluorescence microscope device. In the fluorescence microscope optical system 1, the light emitted from the lamp 9 which is a light source is incident on the fluorescence filter cube 20 after being made substantially parallel by the lens 12. Inside the fluorescent filter cube 20, an excite filter 21 that selectively transmits the excitation wavelength of the fluorescent sample to be observed by the observation sample 6 and light transmitted by the excite filter 21 are reflected so as to be incident on the objective lens 13. A dichroic mirror 22 that transmits the fluorescent wavelength emitted by the fluorescent sample, and an emission filter 23 that transmits the fluorescent wavelength emitted by the fluorescent sample, passes through the excite filter 21, and blocks the excitation light irradiating the sample are arranged. There is. The fluorescence excitation light flux emitted from the lamp light source 9 and reaching the observation sample 6 is shown in 39a, and the objective lens 13 irradiates the excitation light transmitted through the excite filter 21 to the place where the observation sample 6 is to be observed.

The fluorescent light flux from the observation sample 6 to the image pickup element 7 is shown in 39b, and the objective lens 13 collects the fluorescence emitted by the fluorescent sample in the observation sample 6. The fluorescence emitted by the fluorescent sample passes through the emission filter 23 in the filter cube 20 and is imaged on the image pickup device 7 by the image pickup lens 8 to acquire the fluorescence observation image of the observation sample 6 by the image pickup element 7. It is a system.

The focal length of the imaging lens 8 of a general fluorescence microscope is about 200 mm, the length of the objective lens 13 is about 30 to 40 mm, and the diameters of the excite filter 21 and the emission filter 23 are also about 25 mm. The distance from the observation sample 6 to the image pickup element 7 in the optical system 1 is as large as at least about 400 mm.
Here, the fact that the distance from the observation sample 6 to the image pickup element 7 is as large as about 400 mm means that the fluorescent light transmitted through the emission filter 23 and reaching the image pickup element 7 is the same as the example shown in Patent Document 1, the emission filter 23 is used. It is shown that the emission filter 23 is transmitted as substantially parallel light. Further, the excitation light blocked by the emission filter 23 is also incident on the emission filter 23 as substantially parallel light. Since the emission filter 23 is composed of a multilayer dielectric film and its transmission wavelength characteristics change depending on the angle of incident on the transmission filter, it is easy to select the transmission wavelength characteristics that the light incident on the emission filter is almost parallel light. It shows that it can be done.

Next, FIG. 2 shows a compact fluorescence detection optical system 2 using an optical fiber emission end or the like as a light source. This optical system 2 is an optical system used in an apparatus for performing fluorescence diagnosis of the test solution 17. This optical system is an optical system in which the emission end 10 of an optical fiber is arranged at a light emitting point and the light that guides the optical fiber is used as a light source for excitation. The optical path of the excitation light is shown in FIG. 2 as a luminous flux 40a, and the light emitted from the light source 10 is incident on the emission filter 21 after being made substantially parallel by the lens 12. By transmitting through the emission filter 21, the fluorescence excitation wavelength suitable for inspection of the inspection solution 17 is selectively transmitted, reflected by the dichroic mirror 22, and condensed in the inspection solution 17 stored in the chamber 16 by the objective lens 13. .. Here, in the small fluorescence detection optical system 2, since the light source is the emission end 10 of the optical fiber having a small area, the focusing point of the excitation light in the inspection solution 17 is a relatively small region.

FIG. 3 shows an optical path of fluorescence in the compact fluorescence detection optical system 2 using the emission end of an optical fiber or the like as a light source. The optical path of the fluorescent light is shown in FIG. 3 as a light beam 40b. However, since the focusing point of the excitation light in the test solution 17 is a small volume, the emitted fluorescent light is focused by the objective lens 13. After the light becomes substantially parallel and passes through the emission filter 23, it is incident on the light receiving element 15 by the condenser lens 14. Here, in the small fluorescence detection optical system 2 shown in FIGS. 2 and 3, the fluorescent light becomes a substantially parallel luminous flux and passes through the emission filter 23, as in the case of the fluorescence microscope optical system 1 shown in FIG. Therefore, as described above, the fact that the light incident on the emission filter is almost parallel light indicates that the transmitted wavelength characteristic can be easily selected.

Next, in order to have both cost reduction and miniaturization characteristics, FIG. 4 shows an optical system using the light source of the small fluorescence detection optical system shown in FIGS. 2 and 3 as an LED light source. In the small fluorescence detection optical system 3 using the LED light source shown in FIG. 4, the optical path of the excitation light is shown in FIG. 4 as a luminous flux 41a, and the LED light source 11 is the emission end of the optical fiber in the optical system 2 shown in FIG. Unlike No. 10, the light emitting point is large, and the shape is, for example, a square having a side length of 1 mm. Therefore, even if the lens 12 is used, the light cannot be made substantially parallel. Therefore, the luminous flux 41a is incident on the excite filter 21 with a variation in angle, and even if the objective lens 13 is used, it cannot be focused on a small point in the inspection solution 17 stored in the chamber 16. As shown in FIG. 4, a wide area is irradiated.

FIG. 5 shows an optical path of fluorescence in the compact fluorescence detection optical system 3 using the LED light source 11 as a light source. The optical path of the fluorescent light is shown in FIG. 5 as a light beam 41b, but since the focusing point of the excitation light in the test solution 17 is not a small volume, the emitted fluorescent light can be focused by the objective lens 13. It does not become a light beam that can be regarded as substantially parallel light, and after passing through the emission filter 23, it is incident on the light receiving element 15 by the condenser lens 14. Here, FIG. 6 shows an optical path when the angle of the light incident on the emission filter is the shallowest, that is, an optical path having an incident angle farthest from the vertical incident, and the optical path 41c is focused on the objective lens 13. The optical path is such that the optical path crosses the optical path with the optical lens 14, that is, the position through which the objective lens 13 is transmitted and the position through which the condenser lens 14 is transmitted are diagonal positions. Even in the optical system shown in FIG. 3, the focusing point of the excitation light in the test solution 17 is not a focusing point that can be mathematically regarded as zero, but a focusing point having a certain volume. Therefore, it also has a component that becomes an optical path such that the optical path crosses between the objective lens 13 and the condenser lens 14, but the angle of the light incident on the emission filter is the same as the optical path shown in the optical path 41c in FIG. Unlike, it does not become noticeably shallow.

As for the fluorescence characteristics of the fluorescence reagent used in the diagnostic apparatus, the fluorescence emission wavelength is on the longer wavelength side than the excitation wavelength, and the wavelength distributions thereof generally overlap. Therefore, in order to obtain a larger signal, it is desirable to bring the transmission wavelength characteristics of the excite filter and the transmission wavelength characteristics of the emission filter close to each other within a range in which the blocking characteristics of the excitation light can be obtained.

The angle at which the luminous flux shown as the optical path 41c in FIG. 6 is incident on the emission filter is shallower than that in the case shown in FIG. 3, and therefore the angle of incidence on the emission filter 23 is shallower. Therefore, the emission filter of the optical system shown in FIG. It is not possible to obtain the same blocking characteristics for the excitation light as in 23. In the compact fluorescence detection optical system using the LED light sources shown in FIGS. 4, 5 and 6, there is also a method of widening the interval between the transmission wavelength characteristic of the emission filter and the transmission wavelength characteristic of the excite filter as a method of enhancing the blocking characteristic. However, as described above, since the wavelength distributions of the fluorescence excitation wavelength characteristics and the fluorescence emission wavelength characteristics of the fluorescence reagent used in the diagnostic apparatus overlap, a method of widening the interval between these transmission wavelength characteristics is used. Is not desirable because it reduces the amount of light incident on the light detector. On the other hand, there is also a method of using a light source having a large light emitting energy as the light source, but using a light source having a large light emitting energy has a drawback that the cost of the light source is increased.

Further, since the fluorescence detection optical device having the characteristics of low cost and miniaturization is required to have the ability to process a plurality of samples at the same time, the fluorescence detection optical device has a plurality of chambers for storing the test solution. The structure is desirable. In the liquid inspection solution inspection equipment, the containers for arranging the solutions at 9 mm pitch or 4.5 mm pitch are almost standardized, so the fluorescence inspection optical system can be arranged at 9 mm pitch or 4.5 mm pitch. It is desirable that it is an optical system. It is more desirable to use a small and inexpensive LED as a light source because the demands for arranging and equipping a plurality of optical systems in a plurality of devices and for reducing the cost of parts used as a device and reducing the price are further increased. .. Since the device size of an LED having a light emitting portion having a square shape with a side length of 1 mm is often a square chip shape of about 4 mm or less, light emission having a square shape with a side length of 1 mm is obtained. An optical system using an LED having a portion as a light source is suitable for a fluorescence detection optical device capable of inspecting inspection solutions arranged at a pitch of 9 mm or a pitch of 4.5 mm.

US Pat. No. 9,080,978

The present invention has been made in consideration of the above points, and an emission filter is used when manufacturing a compact and low-cost fluorescence detection optical device capable of detecting an amplified gene by using an LED light source as a light source. It is an object of the present invention to provide a fluorescence detection optical device and a method of using the fluorescence detection optical device, which solves the problem of deterioration of the blocking characteristics of the light source.

In order to solve such a problem, in the method of using the fluorescence detection optical device and the fluorescence detection optical device of the present invention, a light-shielding plate having a plane including an optical axis of fluorescent light is arranged between the light detector and the fluorescent reagent. It is possible to block the light incident on the emission filter at a shallow angle, which is generated by using the LED as the light source, and prevent the deterioration of the blocking characteristics of the emission filter.

As described above, the method of using the fluorescence detection optical device and the fluorescence detection optical device of the present invention is a low-cost LED light source as a light source without widening the interval between the transmission wavelength characteristic of the emission filter and the transmission wavelength characteristic of the excite filter. Therefore, a compact and low-cost fluorescence detection optical device can be obtained. The realization of a compact and low-priced fluorescence detection optical device using an LED as a light source makes it possible to reduce the size and price of a device that simultaneously inspects a plurality of test solutions arranged at a pitch of 9 mm or a pitch of 4.5 mm, for example. Great portability and economic benefits can be obtained.

It is a schematic block diagram of the optical system of the fluorescence microscope as a prior art. It is explanatory drawing of the compact fluorescence detection optical system which used the end face of the optical fiber as a light source as a prior art. It is explanatory drawing of the compact fluorescence detection optical system which used the end face of the optical fiber as a light source as a prior art. It is explanatory drawing of the compact fluorescence detection optical system using the LED light source as a prior art. It is explanatory drawing of the compact fluorescence detection optical system using the LED light source as a prior art. It is explanatory drawing of the compact fluorescence detection optical system using the LED light source as a prior art. It is explanatory drawing which shows the principle of the compact fluorescence detection optical system using the LED light source of this invention. It is a schematic block diagram of the optical system of the fluorescence detection optical apparatus of 1st Example of this invention. It is a schematic block diagram of the optical system of the fluorescence detection optical apparatus of 1st Example of this invention. It is a schematic block diagram of the output optical system of the fluorescence detection optical apparatus of the 2nd Example of this invention. It is a schematic block diagram of the optical system of the fluorescence detection optical apparatus of the 2nd Example of this invention. It is a schematic block diagram of the optical system of the fluorescence detection optical apparatus of the 3rd Example of this invention. It is a schematic block diagram of the optical system of the fluorescence detection optical apparatus of the 3rd Example of this invention. It is a schematic block diagram of the optical system of the fluorescence detection optical apparatus which can simultaneously inspect a plurality of arranged inspection solutions of the 4th Example of this invention. It is a schematic block diagram of the optical system of the fluorescence detection optical apparatus which can simultaneously inspect a plurality of arranged inspection solutions of the 5th Example of this invention. 6 is a schematic configuration diagram of an optical system of a fluorescence detection optical device capable of simultaneous inspection of a plurality of arranged inspection solutions according to a sixth embodiment of the present invention.

FIG. 7 shows the principle of a compact fluorescence detection optical system using the LED light source of the present invention. In FIG. 7, it is an optical system in which a light-shielding plate 30 is arranged in order to block an optical path having a shallow incident angle on the emission filter 23 shown as 41c in FIG. The optical path having a shallow incident angle on the emission filter 23 is an optical path having a large angle with respect to the optical axis 60 of the fluorescent light shown in FIG. 7, and the light-shielding plate 30 is arranged on a plane including the optical axis 60 of the fluorescent light. As a result, the optical path of the portion shown by the broken line in FIG. 7 is blocked. Since the plane including the optical axis is parallel to the optical axis, the light-shielding plate 30 does not shield the light traveling parallel to the optical axis, so that the signal light is not significantly reduced. As a result, a low-priced LED light source can be used as the light source without widening the interval between the transmission wavelength characteristic of the emission filter and the transmission wavelength characteristic of the excite filter. Here, the optical axis of the fluorescent light is a straight line indicating the center of the light beam of the fluorescent light, and is the center of the focused spot of the excitation light in the test solution, which is the center of the area where the fluorescent light is emitted, and the condenser lens. Shows a straight line connecting the center of the fluorescent light collected by the light detector.

The fluorescence detection optical device 51 according to the first embodiment of the present invention is shown in FIGS. 8 and 9. FIG. 8 is a view seen from the direction in which the objective lens 13 and the condenser lens 14 overlap, and FIG. 9 is a view seen from a direction orthogonal to FIG. 8, and is a view in which all the component parts can be seen. The component component of the fluorescence detection optical device 51 is a configuration in which an excitation light shading plate 31 is added to a small fluorescence detection optical system using the LED light source shown in FIGS. 3, 4, and 5. That is, an LED light source 11 is used as the light source, the light emitted from the LED light source 11 is incident on the excite filter 21 via the lens 12, and the light transmitted through the excite filter 21 is reflected by the dichroic mirror 22 and then. The inspection solution 17 stored in the chamber 16 is irradiated by the objective lens 13, and the inspection solution 17 is fluorescently excited. The light fluorescently emitted from the test solution 17 is condensed by the objective lens 13, passes through the dichroic mirror 22 and the emission filter 23, is irradiated to the light receiving element 15 by the condenser lens 14, and is converted into an electric signal. Then, in the fluorescence detection optical device 51 according to the first embodiment of the present invention, as shown in FIG. 8, a light-shielding plate 31 including an optical axis 60 of fluorescent light is arranged. The light-shielding plate 31 has a function of light-shielding so that the excitation light that should be originally absorbed by the emission filter does not enter the light-receiving element 15 due to the shallow incident angle on the emission filter 23. Specifically, the light-shielding plate 31 uses a black aluminum plate or the like that has been treated with a glossy alumite of about 0.1 mm to 0.2 mm.

As shown in FIG. 9, the light-shielding plate 31 used in the fluorescence detection optical device 51 according to the first embodiment includes a light-shielding plate 31a between the emission filter 23 and the dichroic mirror 22, an objective lens 13 and a dichroic mirror 22. The shape is divided into a light-shielding plate 31b between the light-shielding plate 31b and a light-shielding plate 31c between the emission filter 23 and the condenser lens 14, but both have a flat plate shape including the optical axis 60 of the fluorescent light. As shown in FIG. 9, the shading plate 31 does not have to have a continuous integrated structure.

The fluorescence detection optical device 52 according to the second embodiment of the present invention is shown in FIGS. 10 and 11. FIG. 10 is a view seen from the direction in which the objective lens 13 and the condenser lens 14 overlap, and FIG. 11 is a view seen from a direction orthogonal to FIG. 10, and is a view in which all the component parts can be seen. Similar to the fluorescence detection optical device 51 according to the first embodiment, the components in the fluorescence detection optical device 52 are excited by a small fluorescence detection optical system using the LED light sources shown in FIGS. 3, 4, and 5. It is a configuration in which a light-shielding plate 32 is added. That is, an LED light source 11 is used as the light source, the light emitted from the LED light source 11 is incident on the excite filter 21 via the lens 12, and the light transmitted through the excite filter 21 is reflected by the dichroic mirror 22 and then. The inspection solution 17 stored in the chamber 16 is irradiated by the objective lens 13, and the inspection solution 17 is fluorescently excited. The light fluorescently emitted from the test solution 17 is condensed by the objective lens 13, passes through the dichroic mirror 22 and the emission filter 23, is irradiated to the light receiving element 15 by the condenser lens 14, and is converted into an electric signal. Then, in the fluorescence detection optical device 52 according to the second embodiment of the present invention, as shown in FIG. 10, a light-shielding plate 32 including an optical axis 60 of fluorescent light is arranged. The light-shielding plate 32 has a function of light-shielding so that the excitation light that should be originally absorbed by the emission filter does not enter the light-receiving element 15 because the light-shielding plate 32 is incident at a shallow angle of incidence on the emission filter 23.

As shown in FIG. 11, the light-shielding plate 32 used in the fluorescence detection optical device 52 according to the second embodiment includes a light-shielding plate 32a between the emission filter 23 and the dichroic mirror 22, an objective lens 13 and a dichroic mirror 22. The shape is divided into a light-shielding plate 32b between the light-shielding plate 32b and a light-shielding plate 32c between the emission filter 23 and the condenser lens 14, but both have a flat plate shape including the optical axis 60 of the fluorescent light. As shown in FIG. 11, the light-shielding plate 32 does not have to have a continuous integrated structure. Further, when the light-shielding plate 31 in the fluorescence detection optical device 51 according to the first embodiment and the light-shielding plate 32 in the fluorescence detection optical device 52 according to the second embodiment are compared, the angles are arranged in different directions by 90 degrees. The light-shielding plate 32b in the fluorescence detection optical device 52 has a shorter shape than the light-shielding plate 31b in the fluorescence detection optical device 51 so as not to block the excitation light of the inspection solution 17. As with the light-shielding plate 31, the light-shielding plate 32 is specifically configured to use a black aluminum plate or the like that has been treated with a black glossy alumite of about 0.1 mm to 0.2 mm.

The fluorescence detection optical device 53 according to the third embodiment of the present invention is shown in FIGS. 12 and 13. The fluorescence detection optical device 53 according to the third embodiment uses both the light-shielding plate 31 in the fluorescence detection optical device 51 according to the first embodiment and the light-shielding plate 32 in the fluorescence detection optical device 52 according to the second embodiment. This is an example. As described above, the light-shielding plate 31 and the light-shielding plate 32 have a function of shielding light from incident light on the emission filter 23 so that the excitation light originally absorbed by the emission filter does not enter the light-receiving element 15. It is the same as the fluorescence detection optical device 51 and the fluorescence detection optical device 52 of the above.

The functions and configurations of the light-shielding plate 31 and the light-shielding plate 32 in the fluorescence detection optical device 53 are the same as the functions and configurations of the light-shielding plate 31 in the fluorescence detection optical device 51 and the light-shielding plate 32 in the fluorescence detection optical device 52. Omit.

FIG. 14 shows a fluorescence detection optical device 54 according to a fourth embodiment of the present invention. The fluorescence detection optical device 54 according to the fourth embodiment is an example in which a plurality of fluorescence detection optical devices 53 according to the third embodiment are arranged.
As the LED light emitting element used as the light source 11, for example, if an LED light emitting element having a square light emitting portion having a side length of 1 mm is used, the size of the chip is about 4 mm, so that the fluorescence detection optical device 54 It is possible to arrange a plurality of fluorescence detection optical devices 53 so as to measure the test solution 17 arranged at a pitch of 9.0 mm or a pitch of 4.5 mm. The light-shielding plate 31 and the light-shielding plate 32 have a function of light-shielding the light-receiving element 15 so that the light-shielding plate 31 and the light-shielding plate 32 are incident at a shallow angle of incidence on the emission filter 23 and the excitation light that should be originally absorbed by the emission filter is not incident on the light-receiving element 15. Is the same as the above-mentioned fluorescence detection optical device 51, fluorescence detection optical device 52, and fluorescence detection optical device 53.

FIG. 15 shows a fluorescence detection optical device 55 according to a fifth embodiment of the present invention. The fluorescence detection optical device 55 according to the fifth embodiment has a structure in which a member having a main surface parallel to the arrangement direction is integrated in the fluorescence detection optical device 54 according to the fourth embodiment. In the fluorescence detection optical device 54, the members having a main surface parallel to the arrangement direction are an excite filter 21, a dichroic mirror 22, an emission filter 23, and a light-shielding plate 32. In the fluorescence detection optical device 55, a plurality of these parts are present. The test solution is not an individual member for each optical system, but an integrated excite filter 26, a dichroic mirror 27, an emission filter 28, and a light-shielding plate 33. By integrating the parts and reducing the number of parts in this way, the fluorescence detection optical device 55 according to the fifth embodiment is cheaper than the fluorescence detection optical device 54 according to the fourth embodiment. It can be manufactured. As described above, the light-shielding plate 31 and the light-shielding plate 33 have a function of shielding light from incident light on the emission filter 28 so that the excitation light originally absorbed by the emission filter does not enter the light-receiving element 15. It is the same as the fluorescence detection optical apparatus 54 of.

FIG. 16 shows the fluorescence detection optical device 56 according to the sixth embodiment of the present invention. The fluorescence detection optical device 56 according to the sixth embodiment has a configuration in which the light-shielding plate 31 is omitted from the fluorescence detection optical device 55 according to the fifth embodiment. Further, a plurality of fluorescence detection optical devices 52 according to the second embodiment shown in FIGS. 10 and 11 are arranged, and a main surface parallel to the arrangement direction of an excite filter 21, a dichroic mirror 22, an emission filter 23, and a light shielding plate 32. The member having the above is not an individual member for each optical system of a plurality of test solutions, but an integrated excite filter 26, a dichroic mirror 27, an emission filter 28, and a light-shielding plate 33. The above-mentioned fluorescence detection optics has a function of shielding the light-shielding plate 33 from incident light on the emission filter 28 so that the excitation light originally absorbed by the emission filter does not enter the light-receiving element 15. It is the same as the light-shielding plate 32 in the device 52.

The present invention relates to a fluorescence detection optical device that enables an LED to be used as a light source in a fluorescence detection optical device that performs a PCR method (polymerase chain reaction) for diagnosing whether or not a virus is infected, and a method for using the same. This makes it easy to reduce the size and price of the fluorescence detection optical device.

1 ... Fluorescence microscope optical system, 2 ... Small fluorescence detection optical system using a small diameter light source, 3, 4 ... Small fluorescence detection optical system using an LED light source, 6 ... Observation sample, 7 ... Image pickup element, 8 ... Imaging lens, 9 ... Lamp, 10 ... Optical fiber connector, 11 ... LED light source, 12 ... Lens, 13 ... Objective lens, 14 ... Condensing lens, 15 ... Light receiving element, 16 ... ... Champer, 17 ... Test solution, 20 ... Filter cube, 21, 26 ... Excite filter, 22, 27 ... Fluorescent mirror, 23, 28 ... Emission filter, 30, 31, 32, 33 ... Excitation light Light-shielding plate, 39a ... Fluorescent excitation light beam that emits a lamp light source and reaches an observation sample, 39b ... Fluorescent light emission beam from an observation sample to an image pickup element, 40a ... Fluorescence excitation light beam that emits an optical fiber connector and reaches an inspection solution, 40b ... Light beam from the test solution to the light receiving element, 41a ... Fluorescent excitation light beam that emits an LED to reach the test solution, 41b ... Light beam from the test solution to the light receiving element, 41c ... From the test solution to the light receiving element Light beam, 51, 52, 53 ... Fluorescence detection optical device, 54, 55, 56 ... Fluorescence detection optical device compatible with multiple reagents, 60 ... Optical axis of fluorescent light

Claims (12)

In a fluorescence detection optical device that irradiates a fluorescent reagent with light emitted from a light source and receives the fluorescent light emitted by the fluorescent reagent with a light detector.
An excite filter that limits the wavelength band of the light applied to the fluorescent reagent is placed between the light source and the fluorescent reagent.
An emission filter that limits the wavelength of the fluorescent light incident on the photodetector is placed between the fluorescent reagent and the photodetector.
A fluorescence detection optical device, characterized in that a light-shielding member having a plane including an optical axis of fluorescent light that emits light from a fluorescent reagent and is incident on a light detector is arranged between the fluorescent reagent and the light detector. In the fluorescence detection optical device, there are a plurality of light sources, light emitted from the plurality of light sources is applied to a plurality of fluorescent reagents corresponding to the individual light sources, and the fluorescent light emitted by the fluorescent reagents corresponds to the individual fluorescent reagents. The fluorescence detection optical device according to claim 1, wherein light is received by a plurality of light detectors. The fluorescence detection optical device according to claim 1 or 2, wherein in the fluorescence detection optical device, the light source is an LED (Light Emitting Diode). The fluorescence detection optical device according to any one of claims 1 to 3, wherein the fluorescence detection optical device has a plurality of members having a plane including an optical axis of fluorescent light emitted by a fluorescent reagent and incident on the light detector. The second to fourth aspects of the fluorescence detection optical device, wherein the excite filter is emitted from a plurality of light sources by the same member and limits the wavelength band of the light irradiated to the fluorescence reagent corresponding to each light source. The fluorescence detection optical device according to any one. 5 according to claim 2, in the fluorescence detection optical device, the emission filter is characterized in that a plurality of fluorescent reagents emit light by the same member and the wavelength of the fluorescent light incident on the light detector corresponding to each fluorescent reagent is limited. The fluorescence detection optical device according to any one of. In a fluorescence detection optical device that irradiates a fluorescent reagent with light emitted from a light source and receives the fluorescent light emitted by the fluorescent reagent with a light detector.
An excite filter that limits the wavelength band of the light applied to the fluorescent reagent is placed between the light source and the fluorescent reagent.
An emission filter that limits the wavelength of the fluorescent light incident on the photodetector is placed between the fluorescent reagent and the photodetector.
By arranging a light-shielding member having a plane including the optical axis of the fluorescent light emitted by the fluorescent reagent and incident on the light detector between the fluorescent reagent and the light detector, the fluorescent reagent was irradiated through the excite filter. A method of using a fluorescence detection optical device, which comprises preventing light from passing through an emission filter. In the method of using the fluorescence detection optical device, there are a plurality of light sources, the light emitted from the plurality of light sources is irradiated to a plurality of fluorescent reagents corresponding to the individual light sources, and the fluorescent light emitted by the fluorescent reagents is emitted by the individual fluorescent reagents. The method of using the fluorescence detection optical device according to claim 7, wherein the light is received by a plurality of light detectors corresponding to the above. The method of using the fluorescence detection optical device according to claim 7 or 8, wherein the light source is an LED (Light Emitting Diode) in the method of using the fluorescence detection optical device. The fluorescence detection optical according to any one of claims 7 to 9, wherein in the method of using the fluorescence detection optical device, there are a plurality of members having a plane including an optical axis of fluorescent light emitted by a fluorescent reagent and incident on a photodetector. How to use the device. 8. In the method of using the fluorescence detection optical device, claim 8 is characterized in that the excite filter is emitted from a plurality of light sources by the same member and limits the wavelength band of light irradiated to the fluorescence reagent corresponding to each light source. 10. The method of using the fluorescence detection optical device according to any one of 10. The method of using the fluorescence detection optical device, wherein the emission filter is characterized in that a plurality of fluorescent reagents emit light by the same member and the wavelength of the fluorescent light incident on the light detector corresponding to each fluorescent reagent is limited. 8. The method of using the fluorescence detection optical device according to any one of 8 to 11.

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