JP2021139794A - Fluid handling device, fluid handling system, and method for producing droplet-containing liquid - Google Patents

Abstract

To provide a fluid handling device capable of stably producing droplet-containing liquid in which liquid droplets are dispersed, even in a case where two kinds of liquid with different viscosities are used.SOLUTION: A fluid handling device has: a first liquid flow path for flowing a first liquid with a first viscosity; a second liquid flow path for flowing a second liquid with a second viscosity higher than the first viscosity; a confluent part; a first transport fluid flow path; a second transport fluid flow path; a generation unit; and a droplet flow path. The first transport fluid flow path and the second transport fluid flow path are configured so that a flow rate of the transport fluid flowing through the second transport fluid flow path is larger than a flow rate of the transport fluid flowing through the first transport fluid flow path when droplet-containing liquid is generated.SELECTED DRAWING: Figure 2

Description

The present invention relates to a fluid handling device, a fluid handling system, and a method for producing a droplet-containing liquid.

In clinical tests, food tests, environmental tests, etc., high-precision analysis of trace amounts of objects to be analyzed such as cells, proteins, and nucleic acids may be required. As one of the methods for analyzing a small amount of an object to be analyzed, a minute droplet (also called a "droplet") having a diameter of 0.1 to 1000 μm is generated from a liquid containing the object to be analyzed and observed. There is a method of performing and analyzing (see, for example, Patent Document 1).

Patent Document 1 describes a microdroplet manufacturing apparatus having a dispersed phase supply channel for flowing a sample, a first microchannel for flowing oil, and a second microchannel for flowing oil. Have been described.

In the apparatus for producing fine droplets described in Patent Document 1, fine droplets are generated by flowing an oil so as to sandwich the flowing sample and dividing the flowing sample.

International Publication No. 2002/068104

Here, it is conceivable that the apparatus for producing fine droplets described in Patent Document 1 produces droplets using two types of liquids having different viscosities. In this case, the solution may come into contact with the inner wall of the flow path, making it impossible to properly produce sample droplets.

Therefore, an object of the present invention is to provide a fluid handling device capable of stably producing a droplet-containing liquid in which liquid droplets are dispersed in a transport fluid even when two types of liquids having different viscosities are used. Another object of the present invention is to provide a fluid handling system having the fluid handling device. Furthermore, another object of the present invention is to provide a method for producing a droplet-containing liquid using a fluid handling device or a fluid handling system.

The fluid handling device of the present invention has a first liquid flow path for flowing a first liquid having a first viscosity and a second liquid flow path for flowing a second liquid having a second viscosity having a viscosity different from the first viscosity. The first liquid flow path and the second liquid flow path are connected to each other, and a merging portion for merging the first liquid and the second liquid, and a first transport fluid flow for flowing the transport fluid. The path, the second transport fluid flow path for flowing the transport fluid, the confluence portion, the first transport fluid flow path, and the second transport fluid flow path are connected, and the first liquid and the said A generation unit for partitioning the second liquid with the transport fluid to generate a droplet-containing liquid in which droplets of the first liquid and the second liquid are dispersed in the transport fluid is connected to the generation unit. The first liquid flow path and the first transport fluid flow path are linear fluid handling devices passing through the confluence part and the generation part. The second liquid flow path and the second transport fluid flow path are arranged on one side when the The first transport fluid flow path and the second transport fluid flow path flow through the second transport fluid flow path when the droplet-containing liquid is generated. The flow rate of the transfer fluid is configured to be larger than the flow rate of the transfer fluid flowing through the first transfer fluid flow path.

The fluid handling system of the present invention includes the fluid handling apparatus of the present invention, the first liquid in the first liquid flow path, the second liquid in the second liquid flow path, and the first transport fluid flow. It has a moving device for moving the transport fluid in the path and the transport fluid in the second transport fluid flow path toward the droplet flow path.

In the method for producing droplets of the present invention, the first liquid and the second liquid are partitioned by a transport fluid using the fluid handling device of the present invention, and the droplets of the first liquid and the second liquid are the transport fluid. A method for producing a droplet-containing liquid dispersed therein, in which, when the droplet-containing liquid is generated, the flow rate of the transported fluid flowing through the second transport fluid flow path is the flow rate of the transport fluid flowing through the first transport fluid flow path. The conveyed fluid is fed so as to be larger than the flow rate.

The fluid handling device of the present invention can stably produce a droplet-containing liquid even when two types of liquids having different viscosities are used.

FIG. 1 is a diagram showing a configuration of a fluid handling system according to an embodiment of the present invention. 2A and 2B are views showing the configuration of a fluid handling device and a substrate. 3A and 3B are diagrams for explaining the effect of the fluid handling system according to the present embodiment.

Hereinafter, the fluid handling apparatus, the fluid handling system, and the method for producing the droplet-containing liquid according to the embodiment of the present invention will be described in detail with reference to the drawings.

(Configuration of fluid handling system and fluid handling device)
FIG. 1 is a schematic view showing the configuration of the fluid handling system 100 according to the first embodiment of the present invention. In FIG. 1, the fluid handling device 110 is shown in cross section. 2A and 2B are views showing the configuration of the fluid handling device 110 and the substrate 112. FIG. 2A is a plan view of the fluid handling device 110, and FIG. 2B is a bottom view of the substrate 112.

As shown in FIG. 1, the fluid handling system 100 includes a fluid handling device 110 and a liquid feeding device 160.

The fluid handling device 110 is composed of a film 111 and a substrate 112, and the film 111 is bonded to one surface of the substrate 112. The region surrounded by the film 111 and the substrate 112 serves as a flow path for flowing the first liquid, the second liquid, the transport fluid, the droplet-containing liquid, and the like. The fluid handling device 110 includes a first liquid introduction unit 121, a first liquid flow path 122, a second liquid introduction unit 123, a second liquid flow path 124, a confluence unit 125, and a first transfer fluid introduction unit 126. It has a first transport fluid flow path 127, a second transport fluid introduction section 128, a second transport fluid flow path 129, a generation section 130, a droplet flow path 131, and a droplet recovery section 132. The first liquid flow path 122 and the first transport fluid flow path 127 are arranged on one side when the fluid handling device 110 is divided into two by a straight line passing through the merging unit 125 and the generating unit 130. The liquid flow path 124 and the second transport fluid flow path 129 are arranged on the other side when the fluid handling device 110 is divided into two by a straight line passing through the merging section 125 and the generating section 130.

The first liquid is a liquid to be sorted as droplets or a liquid containing an object to be sorted by encapsulating the liquid in the droplets. Examples of the first liquid include liquids containing cells, proteins, nucleic acids and the like. In addition, the first liquid may contain a dispersion solvent for dispersing the subject to be sorted such as the above-mentioned cells, proteins, nucleic acids and the like. The first viscosity of the first liquid is not particularly limited. The first viscosity of the first liquid measured at 25 ° C. by a rapid drop viscometer (a method based on the falling ball principle of Heppler) is about 1.0 to 3.0 mPa · s.

The second liquid has a second viscosity higher than the first viscosity. The second liquid is a liquid to be sorted as droplets together with the first liquid, or a liquid containing an object to be sorted by encapsulating the liquid in the droplets. Examples of the second liquid include liquids containing magnetic beads, surfactants, density control liquids, cells, nucleic acids, and the like. Further, the second liquid may contain a dispersion solvent for dispersing the object to be sorted, such as magnetic beads and fluorescent beads. The second viscosity of the second liquid is not particularly limited as long as it is higher than the first viscosity. The second viscosity of the second liquid measured at 25 ° C. with a rapid drop viscometer (a method based on Heppler's falling ball principle) is about 1.5 to 3.0 mPa · s.

The transport fluid separates the sample by colliding with the sample. Examples of transport fluids include oil. Examples of oils include various oils that are liquid at room temperature, such as mineral oil and silicone oil.

The substrate 112 is a transparent substantially rectangular resin substrate. The thickness of the substrate 112 is not particularly limited, but is, for example, 1 to 10 mm. The material of the substrate 112 is not particularly limited and may be appropriately selected from known resins and glass. Examples of materials for the substrate 112 include polyethylene terephthalate, polycarbonate, polymethyl methacrylate, vinyl chloride, polypropylene, polyether, polyethylene, and cycloolefin polymers, cycloolefin copolymers.

The substrate 112 is formed with a plurality of flow path grooves and a plurality of through holes. The substrate 112 has a first through hole 141, a first liquid flow path groove 142, a second through hole 143, a second liquid flow path groove 144, a confluence groove 145, and a third through hole 146. A first transport fluid flow path groove 147, a fourth through hole 148, a second transport fluid flow path groove 149, a generation groove 150, a droplet flow path groove 151, and a fifth through hole 152 are formed. There is. The first liquid flow path groove 142, the second liquid flow path groove 144, the confluence groove 145, the first transfer fluid flow path groove 147, the second transfer fluid flow path groove 149, and the generation groove 150 of the substrate 112. The film 111 is bonded to the surface on which the droplet flow path groove 151 is formed. By joining the film 111 to the substrate 112, the first through hole 141 becomes the first liquid introduction portion 121, the first liquid flow path groove 142 becomes the first liquid flow path 122, and the second through hole 143 becomes the second through hole 143. The liquid introduction section 123, the second liquid flow path groove 144 becomes the second liquid flow path 124, the third through hole 146 becomes the first transfer fluid introduction section 126, and the first transfer fluid flow path groove 147 becomes the first transfer fluid. The flow path 127, the fourth through hole 148 becomes the second transport fluid introduction section 128, the second transport fluid flow path groove 149 becomes the second transport fluid flow path 129, the generation groove 150 becomes the generation section 130, and the droplet flow. The path groove 151 serves as a droplet flow path 131, and the fifth through hole 152 serves as a droplet collection unit 132.

The film 111 is a transparent, substantially rectangular resin film. The thickness of the film 111 is, for example, 30 μm or more and 300 μm or less. Further, the material of the film 111 is not particularly limited. The material of the film 111 can be appropriately selected from known resins. Examples of materials for film 111 include polyethylene terephthalate, polycarbonate, polymethyl methacrylate, vinyl chloride, polypropylene, polyether, polyethylene, cycloolefin polymers, cycloolefin copolymers and the like. The film 111 is bonded to the substrate 112 by, for example, thermocompression bonding, laser welding, an adhesive, or the like.

The first liquid introduction portion 121 is a bottomed recess that is connected to the upstream end of the first liquid flow path 122 and is open to the outside. The first liquid introduction portion 121 is composed of a first through hole 141 formed in the substrate 112 and a film 111 that closes one opening of the first through hole 141. The shape and size of the first liquid introduction portion 121 are not particularly limited, and can be appropriately designed as needed. In the present embodiment, the shape of the first liquid introduction portion 121 is a substantially cylindrical shape. Further, in the present embodiment, the diameter of the first liquid introduction portion 121 is about 2 mm.

The first liquid flow path 122 is a flow path connecting the first liquid introduction section 121 and the confluence section 125. The first liquid flow path 122 is composed of a first liquid flow path groove 142 formed on the substrate 112 and a film 111 that closes the first liquid flow path groove 142. The structure of the first liquid flow path 122 is not particularly limited as long as the first liquid can flow appropriately.

The shape of the cross section of the first liquid flow path 122 is not particularly limited, and may be any shape such as a semicircular shape, a rectangular shape, or a circular shape. The size of the cross section of the first liquid flow path 122 is also not particularly limited. The "cross section of the flow path" means a cross section in a direction orthogonal to the flow direction of the flow path. In the present embodiment, the first liquid flow path 122 has a width of 0.055 mm, a flow path depth of 0.05 mm, and a flow path length of 13 mm.

The second liquid introduction portion 123 is a bottomed recess that is connected to the upstream end of the second liquid flow path 124 and is open to the outside. The second liquid introduction portion 123 is composed of a second through hole 143 formed in the substrate 112 and a film 111 that closes one opening of the second through hole 143. Since the shape and size of the second liquid introduction unit 123 are the same as those of the first liquid introduction unit, the description thereof will be omitted.

The second liquid flow path 124 is a flow path connecting the second liquid introduction section 123 and the merging section 125. The second liquid flow path 124 is composed of a second liquid flow path groove 144 formed on the substrate 112 and a film 111 that closes the second liquid flow path groove 144. The structure of the second liquid flow path 124 is not particularly limited as long as the second liquid can flow appropriately. In the present embodiment, the second liquid flow path 124 has a width of 0.055 mm, a flow path depth of 0.05 mm, and a flow path length of 13 mm.

The merging section 125 is connected to the downstream ends of the first liquid flow path 122 and the second liquid flow path 124, merges the first liquid and the second liquid, and sends the merged liquid to the generating section 130. The merging portion 125 is composed of a merging portion groove 145 formed on the substrate 112 and a film 111 arranged so as to cover the opening of the merging portion groove 145. The downstream end of the confluence 125 is connected to the generator 130.

The first transport fluid introduction unit 126 is a bottomed recess connected to the upstream end of the first transport fluid flow path 127 and open to the outside. The first transfer fluid introduction unit 126 is an introduction port for introducing the transfer fluid into the fluid handling device 110. The first transport fluid introduction unit 126 is composed of a third through hole 146 formed in the substrate 112 and a film 111 that closes one opening of the third through hole 146. The shape and size of the first transport fluid introduction unit 126 are not particularly limited, and can be appropriately designed as needed. In the present embodiment, the shape of the first transport fluid introduction unit 126 is a substantially cylindrical shape. Further, in the present embodiment, the diameter of the first transport fluid introduction portion 126 is about 2 mm.

The first transport fluid flow path 127 is a flow path for connecting the first transport fluid introduction unit 126 and the generation unit 130 and allowing the transport fluid introduced into the first transport fluid introduction section 126 to flow. The first transport fluid flow path 127 is composed of a first transport fluid flow path groove 147 formed on the substrate 112 and a film 111 that closes the first transport fluid flow path groove 147. In the present embodiment, the first transport fluid flow path 127 has a width of 0.1 mm, a flow path depth of 0.05 mm, and a flow path length of 40 mm.

The second transport fluid introduction unit 128 is a bottomed recess connected to the upstream end of the second transport fluid flow path 129 and opened to the outside. The second transport fluid introduction unit 128 is an introduction port for introducing the transport fluid into the fluid handling device 110. The second transport fluid introduction unit 128 is composed of a fourth through hole 148 formed in the substrate 112 and a film 111 that closes one opening of the fourth through hole 148. Since the shape and size of the second transport fluid introduction unit 128 are the same as those of the first transport fluid introduction unit 126, the description thereof will be omitted.

The second transport fluid flow path 129 is a flow path for connecting the second transport fluid introduction unit 128 and the generation unit 130 and allowing the transport fluid introduced into the second transport fluid introduction section 128 to flow. The second transport fluid flow path 129 is composed of a second transport fluid flow path groove 149 formed on the substrate 112 and a film 111 that closes the second transport fluid flow path groove 149. In the present embodiment, the second transport fluid flow path 129 has a width of 0.1 mm, a flow path depth of 0.05 mm, and a flow path length of 36 mm. The second transport fluid flow path 129 is a design value when the flow rate is increased by 10% from the first transport fluid flow path 127.

The generation unit 130 is a region for generating droplets of the first liquid and the second liquid in the transport fluid. The generation unit 130 includes a merging unit 125, a first liquid flow path 122, a second liquid flow path 124, a first transfer fluid flow path 127, a second transfer fluid flow path 129, and a droplet flow path 131. Is arranged to connect. In the generation unit 130, the first liquid and the second liquid merged at the merging unit 125 are partitioned into droplets by the transfer fluid flowing from the first transfer fluid flow path 127 and the second transfer fluid flow path 129, and contain droplets. A liquid is produced. The generated droplet-containing liquid is sent to the droplet flow path 131.

The droplet flow path 131 is a flow path that connects the downstream end of the generation unit 130 and the droplet collection unit 132 and allows the generated droplet-containing liquid to flow. The cross-sectional area of the droplet flow path 131 in the direction orthogonal to the flow direction of the droplet-containing liquid is not particularly limited as long as it is larger than the size of the droplet. The droplet-containing liquid flowing through the droplet flow path 131 is sent to the droplet collection unit 132.

The droplet collecting portion 132 is a bottomed recess that is connected to the downstream end of the droplet flow path 131 and is open to the outside. The droplet collecting portion 132 is composed of a fifth through hole 152 formed in the substrate 112 and a film 111 that closes one opening of the fifth through hole 152. The shape and size of the droplet collecting portion 132 are not particularly limited, and can be appropriately designed as needed. In the present embodiment, the shape of the droplet collecting portion 132 is a substantially cylindrical shape. Further, in the present embodiment, the width of the droplet collecting portion 132 is about 2 mm. A liquid feeding device 160 is connected to the droplet collecting unit 132.

The liquid feeding device 160 includes a first liquid flowing through the first liquid flow path 122, a second liquid flowing through the second liquid flow path 124, a transport fluid flowing through the first transport fluid flow path 127, and a second transport fluid flow. It is a device that moves the transport fluid flowing through the path 129 toward the droplet flow path 131. The liquid feeding device 160 may be a so-called decompression device or a pressurizing device. When the liquid feeding device 160 is a depressurizing device, by making the droplet flow path 131 a negative pressure, the first liquid flowing in the first liquid flow path 122, the second liquid flowing in the second liquid flow path 124, and the second liquid The transport fluid flowing through the 1 transport fluid flow path 127 and the transport fluid flowing through the second transport fluid flow path 129 are moved toward the droplet flow path 131. When the liquid feeding device 160 is a pressurizing device, the first liquid flow path 122, the second liquid flow path 124, the first transport fluid flow path 127, and the second transport fluid flow path 129 are pressurized. As a result, the first liquid flowing in the first liquid flow path 122, the second liquid flowing in the second liquid flow path 124, the transfer fluid flowing in the first transfer fluid flow path 127, and the transfer fluid flowing in the second transfer fluid flow path 129. The transport fluid is moved toward the droplet flow path 131. In the present embodiment, the liquid feeding device 160 is a decompression device. The liquid feeding device 160 includes a lid portion 161, a connecting pipe 162, and a suction pump 163.

The lid portion 161 is connected to the droplet collecting portion 132 and closes the opening of the droplet collecting portion 132. A connecting pipe 162 is connected to the lid portion 161.

The connecting pipe 162 connects the lid portion 161 and the suction pump 163. A lid portion 161 is connected to the upstream end of the connecting pipe 162, and a suction pump 163 is connected to the downstream end.

The suction pump 163 controls the inside of the droplet collecting unit 132 to a negative pressure. The configuration of the suction pump 163 is not particularly limited as long as the above can be exhibited. The suction pump 163 is, for example, a syringe pump.

(How to use the fluid handling system)
Here, a method of using the above-mentioned fluid handling system 100 will be described. First, the first liquid is introduced into the first liquid introduction unit 121 of the fluid handling device 110, the second liquid is introduced into the second liquid introduction unit 123, the transfer fluid is introduced into the first transfer fluid introduction unit 126, and the first 2 The transport fluid is introduced into the transport fluid introduction unit 128. At this time, the first liquid introduction unit 121, the second liquid introduction unit 123, the first transfer fluid introduction unit 126, and the second transfer fluid introduction unit 128 are open, but the upstream end of the first liquid flow path 122 is the first. The upstream end of the second liquid flow path 124 is blocked by the second liquid, and the upstream end of the first transport fluid flow path 127 is blocked by the transport fluid. 2 The upstream end of the transport fluid flow path 129 is blocked by the transport fluid. Next, the droplet collecting portion 132 is closed with the lid portion 161. As a result, the flow path in the fluid handling device 110 is sealed. Finally, the suction pump 163 is driven to create a negative pressure inside the droplet flow path 131. The first liquid flows through the first liquid flow path 122, and the second liquid flows through the second liquid flow path 124. Further, the transport fluid flows through the first transport fluid flow path 127, and the transport fluid flows through the second transport fluid flow path 129. As a result, the first liquid and the second liquid flow into the generation unit 130, and the transport fluid flows into the generation unit 130. Then, the generation unit 130 generates a droplet-containing liquid in which droplets of the first liquid and the second liquid are dispersed in the transport fluid. The generated droplet-containing liquid flows through the droplet flow path 131 and flows into the droplet collection unit 132.

3A and 3B are diagrams for explaining the effect of the fluid handling system according to the present embodiment. FIG. 3A is a schematic view when the droplet-containing liquid is not properly generated, and FIG. 3B is a schematic diagram when the droplet-containing liquid is appropriately generated. As described above, the first viscosity of the first liquid and the second viscosity of the second liquid are different (in the present embodiment, since the second viscosity is higher than the first viscosity, from the first transport fluid flow path 127. If the flow rate of the transfer fluid flowing into the generation unit 130 and the flow rate of the transfer fluid flowing from the second transfer fluid flow path 129 into the generation unit 130 are the same, the highly viscous second liquid comes into contact with the inner wall of the flow path. Liquid droplets may not be properly generated (see FIG. 3A). In this embodiment, the flow rate of the transport fluid flowing through the second transport fluid flow path 129 is the flow rate of the transport fluid flowing through the first transport fluid flow path 127. It is set to be larger than the flow rate of the flowing transport fluid. In the present embodiment, since the liquid feeding device 160 is a decompression device, the cross-sectional area of the first transport fluid flow path 127 is set to be the second transport fluid. It is made larger than the cross-sectional area of the flow path 129 so that the flow rate of the transfer fluid flowing through the second transfer fluid flow path 129 is larger than the flow rate of the transfer fluid flowing through the first transfer fluid flow path 127. Since the transport fluid flowing through the second transport fluid flow path 129 flows so as to separate the second liquid and the first liquid from the side wall of the flow path, a droplet-containing liquid can be stably generated (see FIG. 3B).

When the liquid feeding device 160 is a pressurizing device, even if the cross-sectional area of the downstream end of the first transport fluid flow path 127 and the cross-sectional area of the downstream end of the second transport fluid flow path 129 are the same, the first 2 By making the pressure inside the transport fluid flow path 129 higher than the pressure inside the first transport fluid flow path 127, the flow rate of the transport fluid flowing through the second transport fluid flow path 129 can be changed to the first transport fluid flow path. It can be made higher than the flow rate of the transport fluid flowing through 127.

(effect)
As described above, according to the present invention, even if two kinds of liquids having different viscosities are used, the sample is divided by the transport fluid to stably produce a droplet-containing liquid in which the droplets of the sample are dispersed in the transport fluid. can.

In the present embodiment, the confluence unit 125 is connected to the generation unit 130, but the confluence unit 125 and the generation unit 130 may be integrally formed. In this case, the generation unit 130 includes a first liquid flow path 122, a second liquid flow path 124, a first transport fluid flow path 127, a second transport fluid flow path 129, and a droplet flow path 131. Be connected.

The fluid handling device and the fluid handling method of the present invention can be applied to, for example, clinical tests, food tests, environmental tests, and the like.

100 Fluid handling system 110 Fluid handling device 111 Film 112 Substrate 121 1st liquid introduction part 122 1st liquid flow path 123 2nd liquid introduction part 124 2nd liquid flow path 125 Confluence part 126 1st transfer fluid introduction part 127 1st transfer Fluid flow path 128 2nd transfer fluid introduction part 129 2nd transfer fluid flow path 130 Generation part 131 Droplet flow path 132 Droplet recovery part 141 1st through hole 142 1st liquid flow path groove 143 2nd through hole 144 2nd Liquid flow path groove 145 Confluence groove 146 3rd through hole 147 1st transfer fluid flow path groove 148 4th through hole 149 2nd transfer fluid flow path groove 150 Generation groove 151 Droplet flow path groove 152 5th through hole 160 Liquid device 161 Lid 162 Connection pipe 163 Suction pump

Claims (6)

A first liquid flow path for flowing a first liquid having a first viscosity,
A second liquid flow path for flowing a second liquid having a second viscosity having a viscosity different from that of the first viscosity,
A confluence portion for connecting the first liquid flow path and the second liquid flow path and merging the first liquid and the second liquid.
The first transport fluid flow path for flowing the transport fluid and
A second transport fluid flow path for flowing the transport fluid and
The merging portion, the first transport fluid flow path, and the second transport fluid flow path are connected, and the first liquid and the second liquid are partitioned by the transport fluid, and the first liquid and the first liquid are divided. 2 A generation unit for generating a droplet-containing liquid in which liquid droplets are dispersed in the transport fluid, and
A droplet flow path connected to the generation unit and for flowing the droplet-containing liquid,
Have,
The first liquid flow path and the first transport fluid flow path are arranged on one side when the fluid handling device is divided into two by a straight line passing through the merging part and the generating part.
The second liquid flow path and the second transport fluid flow path are arranged on the other side when the fluid handling device is divided into two by a straight line passing through the merging part and the generating part.
In the first transport fluid flow path and the second transport fluid flow path, the flow rate of the transport fluid flowing through the second transport fluid flow path when the droplet-containing liquid is generated is the flow rate of the first transport fluid flow path. It is configured to be greater than the flow rate of the transport fluid flowing.
Fluid handling device. With the first liquid introduction part connected to the upstream end of the first liquid flow path,
A second liquid introduction portion connected to the upstream end of the second liquid flow path,
The first transport fluid introduction unit connected to the upstream end of the first transport fluid flow path,
A second transport fluid introduction unit connected to the upstream end of the second transport fluid flow path,
A droplet collection unit connected to the downstream end of the droplet flow path,
Have more,
The fluid handling device according to claim 1. The fluid handling device according to claim 1 or 2.
The first liquid in the first liquid flow path, the second liquid in the second liquid flow path, the transfer fluid in the first transfer fluid flow path, and the second transfer fluid flow path. A moving device that moves the transport fluid of the above toward the droplet flow path, and
Have,
Fluid handling system. By creating a negative pressure inside the droplet flow path, the moving device has the first liquid in the first liquid flow path, the second liquid in the first liquid flow path, and the first liquid. The fluid handling system according to claim 3, which is a decompression device for moving the transport fluid in the transport fluid flow path and the transport fluid in the second transport fluid flow path toward the droplet flow path. .. The moving device in the first liquid flow path by pressurizing the inside of the first liquid flow path, the second liquid flow path, the first transport fluid flow path, and the second transport fluid flow path. The droplets of the first liquid, the second liquid in the sample flow path, the transport fluid in the first transport fluid flow path, and the transport fluid in the second transport fluid flow path. The fluid handling system according to claim 3, which is a pressurizing device that moves toward a flow path. Using the fluid handling device according to claim 1 or 2, the first liquid and the second liquid are partitioned by the transport fluid, and the droplets of the first liquid and the second liquid are the transport fluid. A method for producing a droplet-containing liquid dispersed therein.
When the droplet-containing liquid is generated, the transport fluid is applied so that the flow rate of the transport fluid flowing through the second transport fluid flow path is larger than the flow rate of the transport fluid flowing through the first transport fluid flow path. Send liquid,
A method for producing a droplet-containing liquid.

Images