JP2021130623A - Processed pollen lump, method for processing pollen lump, and anti-aging composition - Google Patents

Abstract

To provide a processed pollen lump that can be used for various applications including food and drink, a method for processing a pollen lump, and an anti-aging composition.SOLUTION: A processed pollen lump is obtained by: processing a pollen lump suspended in solvent with at least one enzyme selected from cellulase and protease, and concentrating or drying the processed pollen lump without liquid-solid separation.SELECTED DRAWING: None

Description

The present invention relates to a pollen-loaded product, a pollen-loaded treatment method, and an anti-aging composition.

In general, pollen load is a honey bee that obtains pollen from the stamens of plants, rolls it with honey or saliva and hardens it into a dumpling, and contains a wide variety of nutrients such as high-quality proteins, vitamins and minerals. Also called "perfect food". The pollen load is easily collected from honeybees by a collector or the like, and is mainly ingested as a dietary supplement or the like.

Conventionally, it is known that pollen load has some pharmacological effects. As an invention utilizing the pharmacological effect of pollen load, for example, the compositions disclosed in Patent Documents 1 and 2 are known. Patent Document 1 discloses a bone mass-enhancing composition containing a pollen load as an active ingredient. Patent Document 2 discloses a composition for preventing / treating a diabetic disease containing pollen load as an active ingredient.

JP-A-2007-016014 Japanese Unexamined Patent Publication No. 2008-105982

The present inventors have sought a new physiological action of pollen load. As a result, it has been discovered that the treatment of pollen loads with cellulase or protease increases a specific active ingredient.

An object of the present invention is to provide a pollen-loaded product, a pollen-loaded treatment method, and an anti-aging composition that can be used for various purposes such as food and drink.

The present invention has been made based on the finding that the elution amount of polyamines can be increased by treating the pollen load with cellulase or protease.
In order to achieve the above object, the pollen-loaded product of one aspect of the present invention treats the pollen-loaded product suspended in a solvent with at least one enzyme selected from cellulase and protease, and concentrates without solid-liquid separation. Alternatively, it is characterized in that it is obtained by drying treatment.

The enzyme may be a cellulase or a neutral protease.
The pollen load may be further treated with a wet ultra-high pressure particle collision homogenizer.

Another aspect of the present invention for treating the pollen load in order to achieve the above object is a step of adding a solvent to the pollen load and treating with at least one enzyme selected from cellulase and protease, without solid-liquid separation. It is characterized by including a step of concentrating or drying.

In order to achieve the above object, another aspect of the present invention is an anti-aging composition, in which a pollen load suspended in a solvent is treated with at least one enzyme selected from cellulase and protease, and solid-liquid separation is performed. It is characterized by containing a pollen-loaded processed product obtained by concentration or drying treatment as an active ingredient.

According to the present invention, it can be used for various purposes such as food and drink.

Hereinafter, an embodiment in which the pollen-loaded processed product of the present invention is embodied will be described. In the pollen load processed product of the present embodiment, the pollen load as a raw material is suspended in a solvent, treated with at least one enzyme selected from cellulase and protease, and concentrated or dried without solid-liquid separation. It is the obtained product.

In this pollen-loaded product, the amount of polyamine eluted is larger than that in the pollen-loaded product treated with a homomixer or the like, which is a conventional wet pulverizer. The type of polyamine is not particularly limited, and typical polyamines include spermidine, spermine, and putrescine. In the pollen-packed product of the present invention, it is preferable that the total amount of these polyamines eluted is increased.

As described above, the pollen load, which is the raw material, is obtained by honeybees obtaining pollen from the stamens of plants, rolling it with honey or saliva and hardening it into dumplings, and contains a wide variety of nutrients such as vitamins and minerals. The place of origin of the pollen load is not particularly limited, and may be, for example, any of Asian countries such as Japan and China, Latin American countries such as Brazil, North American countries such as the United States and Canada, European countries such as Spain, and Oceanian countries. .. Especially when using foreign pollen cargo, when transporting raw materials from the production area to the factory, gas replacement packaging is performed by vacuum treatment, inert gas, etc. in order to prevent the pollen cargo during transportation from oxidizing and the generation of insects. It is preferable to apply. For example, oxygen in the container is removed by vacuum treatment for 10 to 20 seconds, and more preferably nitrogen or a mixed gas of nitrogen and carbon dioxide is filled in the container for 1 to 5 seconds, and gas replacement packaging is performed. The process of applying can be carried out.

The plant of origin of pollen, which is the raw material of the pollen load, is not particularly limited, and any plant collected by honeybees can be used. Examples of plants of origin of pollen include Rock-roses, Ericaceae, Labiatae, Borages, Beech, Kiku, Mokusei, Abranaceae, Beans, Rosaceae, Salixaceae and the like. Among these, the family Helianthemum and Brassicaceae are preferable from the viewpoint of availability. Examples of the Helianthemum family include Cistus genus Jara and the like. Examples of Brassicaceae include Brassicaceae rapeseed, Radishes radish and the like. The method for collecting the pollen load is not particularly limited, and a known method can be appropriately adopted. For example, a method using a pollen collector attached to the entrance / exit of the nest box and provided with a grid-like stripped perforated plate, a method of directly collecting the pollen load attached to the nest plate or the bee, and the like can be mentioned. The obtained pollen load may be sorted before the enzyme treatment, removal of impurities such as dust mixed in at the time of collection, cleaning treatment and the like. In addition to direct visual sorting, foreign matter can be removed by passing through a specific gravity sorter or a wind power sorter.

The pollen load crushing treatment using cellulase is a treatment of hydrolyzing the glycosidic bonds of β-1,4-glucan such as cellulose constituting the cell wall of the pollen load to reduce the molecular weight. Cellulase includes exo-type cellulase that hydrolyzes from the terminal of β-1,4-glucan and endo-type cellulase that decomposes from the middle position of β-1,4-glucan, and any cellulase can be used. Further, for cellulase, an acidic cellulase having an optimum pH near acidic (for example, pH less than 6.0), near neutral (for example, pH 5.0 to 9.0), and near alkaline (for example, pH 8.0 or higher). , Neutral cellulase and alkaline cellulase are present respectively, but any cellulase can be used. Specific examples of cellulase include acidic cellulase derived from Jiuqu. As an acidic cellulase derived from Jiuqu, the Sumiteam series (manufactured by Shin Nihon Kagaku Kogyo Co., Ltd.) can be used as a commercially available product.

In the treatment of pollen load using cellulase, a reaction solution is first prepared by suspending the raw material pollen load in a solvent. The reaction solution contains water (or a buffer solution) as a solvent for suppressing the increase in viscosity caused by the raw material and rapidly advancing the cellulase treatment. It is desirable that the reaction solution contains 2 to 15 times, preferably 2 to 14 times, more preferably 3 to 10 times the amount of water (or buffer solution) with respect to the mass of the raw material pollen load. When a solvent in an amount of twice or more the mass of the raw material pollen load is added, the increase in viscosity of the reaction solution due to the raw material pollen load can be sufficiently suppressed, and the cellulase treatment can proceed rapidly. On the contrary, when the solvent is added in an amount of 15 times or less the mass of the raw material pollen load, the treatment time can be shortened when the pollen load processed product obtained through each of the subsequent treatments is pulverized.

The temperature conditions for the treatment using cellulase are appropriately set depending on the type of enzyme. For example, when acidic cellulase derived from Jiuqu is used, it is preferably 40 to 60 ° C, more preferably 40 to 55 ° C, and even more preferably. It is 50 to 55 ° C. The treatment time for the treatment using cellulase is appropriately set depending on the type of enzyme. For example, when acidic cellulase derived from Jiuqu is used, it is preferably 0.5 to 12 hours, more preferably 1 to 10 hours, and further. It is preferably 2 to 6 hours. In this cellulase treatment, it is desirable to immediately heat the treated reaction solution at 85 to 100 ° C. for 5 to 60 minutes to inactivate cellulase.

The protease treatment is a treatment for hydrolyzing the peptide bonds of proteins contained in the raw material pollen load to reduce the molecular weight. Proteases include exo-type proteases that hydrolyze from the end of the peptide and endo-type proteases that hydrolyze from the intermediate position of the peptide, and any protease can be used. Further, as a protease, an acidic protease having an optimum pH near acidic (for example, pH less than 6.0), near neutral (for example, pH 5.0 to 9.0), and near alkaline (for example, pH 8.0 or higher). , Neutral protease and alkaline protease are present respectively, but any of these proteases can be used. Further, as the protease, there are proteases derived from various substances such as those derived from microorganisms, animals, plants, etc., and proteases derived from any of them can be used. Among these proteases, acidic proteases or neutral proteases having excellent polyamine elution are preferable.

Specific examples of the acidic protease include an acidic protease derived from Aspergillus niger. As the neutral protease, specifically, a neutral protease derived from Bacillus subtilis, a neutral protease derived from Aspergillus oryzae, and a neutral protease derived from Bacillus stearothermophilus. Neutral proteases and proteases derived from papaya can be mentioned. Specific examples of the alkaline protease include an alkaline protease derived from Bacillus licheniformis.

Protease treatment using various proteases is carried out by incubating a reaction solution containing a raw material pollen load, a protease, and water (or a buffer solution) under reaction conditions corresponding to the various proteases. It may be carried out by further adding a protease to the reaction solution after the cellulase treatment and incubating under the reaction conditions according to the protease. The treatment time of the protease treatment is appropriately set depending on the reaction temperature, the titer of the enzyme, etc., but is preferably 1 to 30 hours, more preferably 4 to 20 hours. The treatment pH is preferably around the optimum pH of each enzyme. In this protease treatment, it is desirable to immediately heat the reaction solution after incubation at 85 to 100 ° C. for 5 to 60 minutes to inactivate the protease.

The above-mentioned cellulase or protease treatment may be performed on either one or both. When treating with both enzymes, either may be performed first, or may be performed at the same time if the conditions are met. The pollen-loaded product is preferably one treated with cellulase and a neutral protease in the above-mentioned enzyme treatment. By performing such a treatment, the elution amount of polyamine can be further increased.

The pollen-loaded product may be further subjected to a wet ultra-high pressure particle collision type homogenizer treatment. By adding a wet ultra-high pressure particle collision type homogenizer treatment, the elution amount of polyamine can be further increased. The wet ultra-high pressure particle collision homogenizer treatment may be performed in any of the steps before or after the enzyme treatment described above, but in order to improve the efficiency of the enzyme treatment and further increase the elution amount of the polyamine, the enzyme is used. It is preferable to carry out before the treatment.

The wet ultra-high pressure particle collision type homogenizer is a crushing device that pressurizes a sample solution to an ultra-high pressure to collide the samples with each other and pulverize them. Treatment with this homogenizer destroys the cell walls that make up pollen in particular. Examples of commercially available products include a wet atomizing device "Starburst" manufactured by Sugino Machine Limited. Starburst crushes an object by causing particles pressurized to ultra-high pressure (for example, 200 MPa or more) to collide obliquely at ultra-high speed.

In the treatment of pollen load using a wet ultra-high pressure particle collision type homogenizer, a treatment solution is prepared by first putting a treatment sample containing pollen load into a predetermined crushing container together with a treatment solvent and suspending the sample for a predetermined time. do. As the treatment solvent, water or a water / hydrophilic organic solvent mixed solution is used. Examples of the hydrophilic organic solvent include lower alcohols such as ethanol, methanol and isopropanol having the property of being soluble in water, propylene glycol (PG), 1,3-butylene glycol (BG), polyethylene glycol (PEG), glycerin and the like. Polyhydric alcohol, acetone, ethers, chloroform and the like can be appropriately selected and used. Among these, water or a water / ethanol mixed solution is applied from the viewpoint of bioapplicability. The amount of the treatment solvent used is appropriately set according to the type of apparatus and the like, but is preferably 0.5 to 10 times, more preferably 1 to 5 times the mass of the pollen load (solid content). Is. When the amount of the solvent used is 0.5 times or more, the fluidity can be improved and the pulverization efficiency can be further improved. On the other hand, when the volume of the treatment solvent used is 10 times or less, it is not necessary to increase the size of the apparatus, the treatment time in the subsequent steps such as concentration and drying can be further shortened, and workability can be further improved. Additives such as a pH adjuster and a surfactant may be added to the treatment solvent to improve the stability of the solvent during treatment.

As the pressure treatment condition using a commercially available wet ultra-high pressure particle collision type homogenizer, a condition of preferably 100 MPa or more, more preferably 150 MPa or more, still more preferably 200 MPa or more is adopted. When the pressure treatment condition is 100 MPa or more, the crushing efficiency of the pollen load is improved. The upper limit of the pressure of the pressurizing treatment is not particularly limited, and is appropriately determined according to the upper limit of the pressurization of the device of the wet ultrahigh pressure particle collision type homogenizer. Further, the number of pressurization treatments using the wet ultra-high pressure particle collision type homogenizer may be repeated not only once (pass) but also twice or more (pass), if necessary.

When the raw material pollen load before the enzyme treatment is used as the treatment sample, it is preferable that the raw material pollen load is pretreated in advance by a wet pulverizer such as a homomixer. By performing such a treatment, the granules of the pollen load can be loosened, and the treatment with the wet ultra-high pressure particle collision type homogenizer can be made more efficient. Examples of the wet pulverizer include a homomixer, a homogenizer, and a homodisper. These devices are carried out by putting a pollen load into a predetermined crushing container together with a stirring solvent and stirring for a predetermined time. Examples of the stirring solvent include the above-mentioned homogenizer treatment solvent.

As described above, the pollen load is treated with cellulase or protease, and if necessary, a wet ultra-high pressure particle collision type homogenizer treatment is added, and then a step of concentration or drying treatment is performed without solid-liquid separation. The pollen load-treated product is preferably heat-treated after the above treatment. The heat treatment kills microorganisms such as molds and bacteria and improves bioapplicability. It is also carried out to eliminate the fishy odor of pollen loads, especially when taken orally. Further, the heat treatment may be performed at the same time as the inactivation treatment of the enzyme when the enzyme treatment using the above-mentioned cellulase or protease is performed. The heat treatment is preferably carried out in the state of a liquid dispersion liquid from the viewpoint of being homogeneously and evenly performed. As the dispersion liquid for heat treatment, the solid content concentration of the pollen load may be appropriately adjusted by concentration, dilution, etc. before the heat treatment, but from the viewpoint of improving the treatment efficiency, the treatment liquid treated with a wet homogenizer or enzyme treatment. Is more preferable to be heat-treated as it is.

The heat treatment temperature can be appropriately set within a range in which the sterilization treatment effect can be obtained while suppressing the denaturation and alteration of the pollen load in consideration of the treatment time and the like. It is preferably 70 to 95 ° C. When the treatment temperature is 70 ° C. or higher, the sterilization efficiency can be further improved. On the other hand, when the treatment temperature is 95 ° C. or lower, the denaturation / alteration of the pollen load can be further suppressed.

The heat treatment time can be appropriately set within a range in which the sterilization treatment effect can be obtained while suppressing the denaturation and alteration of the pollen load in consideration of the treatment temperature and the like, but is preferably 5 to 60 minutes. When the treatment time is 5 minutes or more, the sterilization efficiency can be further improved. On the other hand, when the treatment time is 60 minutes or less, the denaturation / alteration of the pollen load can be further suppressed.

The heat-treated pollen load-treated product is concentrated by evaporating water, or dried and pulverized. For the concentration and drying of the treatment liquid, known vacuum concentration, membrane concentration, freeze concentration, vacuum drying, spray drying, or freeze drying can be adopted.

The pollen-loaded product is preferably applied to foods and drinks and the like. In the case of food and drink, the pollen-loaded product can be used as the food and drink itself, or by blending with various food materials or beverage materials. The form of the food or drink is not particularly limited and may be in the form of liquid, powder, gel or solid, and the dosage form may be powder, granules having a predetermined shape, tablets, capsules (hard capsules, etc.). It may be either a soft capsule) or a drink. As the food and drink, other ingredients such as gelling agent-containing foods, sugars, flavors, sweeteners, fats and oils, base materials, excipients, food additives, auxiliary materials, bulking agents and the like may be appropriately blended. The use of foods and drinks is not particularly limited, and can be applied as so-called general foods, health foods, functional foods, dietary supplements, supplements, foods for specified health use, foods with functional claims, and foods for the sick.

The operation of the pollen load processed product of the present embodiment will be described.
In the pollen-loaded product of the present embodiment, the elution amount of polyamine is significantly increased as compared with the untreated pollen-loaded product. Polyamines have traditionally had anti-aging, hair quality improving, skin-beautifying, cognitive function improving, arteriosclerosis improving, cardiovascular disease prevention, neuroprotective, anti-inflammatory, and antitumor effects. Have. Therefore, the pollen-loaded product of the present embodiment has an anti-aging composition, a hair quality improving composition, a skin-beautifying composition, a cognitive function improving composition, and an arteriosclerosis improving composition for the purpose of exerting these effects. It may be applied as a composition for use, a composition for prevention of cardiovascular disease, a composition for neuroprotection, a composition for anti-inflammatory, and a composition for antitumor. When displaying the intended use in food and drink, it includes not only the display on packages such as packaging and containers, but also the display on advertising media such as pamphlets. In addition to the above-mentioned indications such as anti-aging action, the indication contents for various uses of the pollen cargo-treated product of the present embodiment suggest improvement, prevention, prevention of deterioration, or delay of symptoms of each symptom such as skin aging. Display is also included.

The effect of the pollen cargo treated product of the present embodiment will be described.
(1) In the pollen-loaded product of the present embodiment, the pollen-loaded product is treated with cellulase or protease, so that the pollen contents are easily eluted. In addition, the amount of polyamine eluted in this pollen-loaded product is larger than that in the pollen-loaded product treated with a conventional homomixer or the like. Therefore, the amount of polyamine absorbed in the living body can be increased, and the indications and effects derived from polyamine that are useful for the living body can be effectively exhibited. Therefore, it can be used for various fields such as foods and drinks and pharmaceuticals.

(2) The pollen-loaded processed product is not subjected to solid-liquid separation treatment, for example, filtration treatment, filter treatment, centrifugation or the like in the manufacturing process. Therefore, the amount of polyamine eluted can be increased. In addition, insoluble components such as dietary fiber and components adsorbed on dietary fiber are also suspended in the solvent. Therefore, by not performing the separation treatment of the insoluble fraction, those nutritional components can be ingested together.

(3) Further, when a wet ultra-high pressure particle collision type homogenizer is used in the pollen load destruction treatment, the amount of polyamine eluted from the pollen load treated product can be further increased. Further, the wet ultra-high pressure particle collision type homogenizer has less contamination and is easier to process than, for example, a bead mill. Further, as compared with a dry pulverization method such as a jet mill, the collision efficiency between particles is high, and the treatment can be completed in a short time.

(4) When the pollen-packed product of the present embodiment is subjected to protease treatment, the protein is reduced to, for example, a molecular weight of 10,000 or less. Therefore, since the contents of free amino acids and peptides in the pollen load increase, the flavor peculiar to the pollen load is masked, and the taste improving effect can be obtained. Further, when dispersed in a liquid, the dispersibility can be improved.

The above embodiment can be modified as follows. The above embodiment and the following modified examples can be implemented in combination with each other within a technically consistent range.
-The pollen-packed product obtained by the above embodiment can be applied as a pharmaceutical product, a quasi-drug, a cosmetic product, etc., in addition to the above-mentioned food and drink.

Hereinafter, the above-described embodiment will be described in more detail with reference to test examples, but the present invention is not limited thereto.
(Example 1)
First, 2.5 g of the raw material pollen load (produced in Spain) was suspended in water so as to have a content of 20% by mass, and a reaction solution was prepared. Next, 0.02 g of cellulase (0.8% by mass with respect to the raw material pollen load) was added and reacted at 50 ° C. for 4 hours to destroy the cell wall of the pollen load. Sumiteam (manufactured by Shin Nihon Kagaku Kogyo Co., Ltd.) was used as cellulase.

Next, the obtained destructive product was heat-treated for the purpose of sterilization and inactivation of the enzyme. The heat treatment was carried out in a suspended state at 80 ° C. for 60 minutes. Next, it was cooled to 50 ° C. and dried in a solid state using a freeze dryer. The obtained solid matter was crushed with a hammer mill to obtain a powdered pollen-loaded product of Example 1.

(Example 2)
First, 2.5 g of the raw material pollen load (produced in Spain) was suspended in water so as to have a content of 20% by mass, and a reaction solution was prepared. Next, 0.02 g (0.8% by mass with respect to the raw pollen load) of a neutral prosthesis was added and reacted at 50 ° C. for 4 hours to destroy the cell wall of the pollen load. Protease M "Amano" SD (manufactured by Amano Enzyme) was used as a neutral protease.

Next, the obtained fracture-treated product was heat-treated, dried, and pulverized in this order by the same method as in Example 1 to obtain a powdered pollen-loaded product of Example 2.
(Example 3)
First, a reaction solution was prepared so that the raw material pollen load (produced in Spain) was 20% by mass. Next, 0.02 g of cellulase (0.8% by mass with respect to the raw material pollen load) was added and reacted at 50 ° C. for 4 hours to destroy the cell wall of the pollen load. Sumiteam (manufactured by Shin Nihon Kagaku Kogyo Co., Ltd.) was used as cellulase.

Next, 0.02 g (0.8% by mass with respect to the raw pollen load) of acidic protease was further added to the obtained reaction solution, and decomposition treatment was carried out at 50 ° C. for 16 hours. Protease HF "Amano" 150SD (manufactured by Amano Enzyme) was used as the acidic protease.

Next, the obtained fracture-treated product was heat-treated, dried, and pulverized in this order by the same method as in Example 1 to obtain a powdered pollen-loaded product of Example 3.
(Example 4)
To 90 kg of the raw material pollen load (made in Spain), 135 kg of water as a stirring solvent (1.5 times the amount of the raw material pollen load) was put into a 200 L drum. A TK HOMO MIXER manufactured by Tokushu Kagaku Kogyo Co., Ltd. was used as a homomixer, and a wet pulverization treatment was performed at room temperature (25 ° C.) for 10 minutes. Next, a wet atomizer Starburst manufactured by Sugino Machine Limited was used as a wet ultra-high pressure particle collision homogenizer to destroy the cell wall of the pollen load. The pressure during the treatment was adjusted to 245 MPa, and two-pass treatment was performed at room temperature (25 ° C.).

Next, a part of the obtained treatment liquid was taken out, 0.02 g of a neutral protease (0.8% by mass with respect to the raw pollen load) was further added, and decomposition treatment was carried out at 50 ° C. for 16 hours. Protease M "Amano" SD (manufactured by Amano Enzyme) was used as a neutral protease.

Next, the obtained fracture-treated product was heat-treated, dried, and pulverized in this order by the same method as in Example 1 to obtain a powdered pollen-loaded product of Example 4.
(Example 5)
First, 2.5 g of the raw material pollen load (produced in Spain) was suspended in water so as to have a content of 20% by mass, and a reaction solution was prepared. Next, 0.02 g of cellulase (0.8% by mass with respect to the raw material pollen load) was added and reacted at 50 ° C. for 4 hours to destroy the cell wall of the pollen load. Sumiteam (manufactured by Shin Nihon Kagaku Kogyo Co., Ltd.) was used as cellulase.

Next, 0.02 g (0.8% by mass with respect to the raw pollen load) of neutral protease was further added to the obtained reaction solution, and decomposition treatment was carried out at 50 ° C. for 16 hours. Protease M "Amano" SD (manufactured by Amano Enzyme) was used as a neutral protease.

Next, the obtained fracture-treated product was heat-treated, dried, and pulverized in this order by the same method as in Example 1 to obtain a powdered pollen-loaded product of Example 5.
(Comparative Example 1)
As the raw material pollen load, the same material as in Example 1 was used. First, the raw pollen load was sterilized with superheated steam exceeding 100 ° C. under the condition of 10 minutes. Next, the obtained processed product was crushed with a hammer mill or the like to obtain a powdered pollen-loaded processed product of Comparative Example 1.

(Comparative Example 2)
As the raw material pollen load, the same material as in Example 1 was used. First, 135 kg of water as a stirring solvent (1.5 times the amount of the raw pollen load) was put into a 200 L drum in 90 kg of the raw pollen load. A TK HOMO MIXER manufactured by Tokushu Kagaku Kogyo Co., Ltd. was used as a homomixer, and a wet pulverization treatment was performed at room temperature (25 ° C.) for 10 minutes. Next, the obtained pulverized product was heated (sterilized). The heat treatment was carried out under the condition of heating in a suspended state at 90 ° C. for 15 minutes. Next, drying and pulverization treatments were sequentially carried out in the same manner as in Example 1 to obtain a powdered pollen-loaded product of Comparative Example 2.

(Comparative Example 3)
To 90 kg of the raw material pollen load (made in Spain), 135 kg of water as a stirring solvent (1.5 times the amount of the raw material pollen load) was put into a 200 L drum. A TK HOMO MIXER manufactured by Tokushu Kagaku Kogyo Co., Ltd. was used as a homomixer, and a wet pulverization treatment was performed at room temperature (25 ° C.) for 10 minutes. Next, a wet atomizer Starburst manufactured by Sugino Machine Limited was used as a wet ultra-high pressure particle collision homogenizer to destroy the cell wall of the pollen load. The pressure during the treatment was adjusted to 245 MPa, and two-pass treatment was performed at room temperature (25 ° C.).

Next, the obtained fracture-treated product was heat-treated, dried, and pulverized in this order by the same method as in Example 1 to obtain a powdered pollen-loaded product of Comparative Example 3.
(Comparative Example 4)
In the configuration of Example 5, after treatment with a neutral protease, pressure filtration was then performed using diatomaceous earth and a filter press to separate insoluble components, and only the supernatant was recovered.

Next, the supernatant of the obtained fracture-treated product was heat-treated, dried, and pulverized in this order by the same method as in Example 1 to obtain a powdered pollen-loaded product of Comparative Example 4. ..
(Elution amount of polyamine)
In the treated product of each example, the amount of polyamine eluted in the aqueous solvent was determined. First, 0.2 g of the treated product of each example was suspended in 25 mL of a perchloric acid solution, subjected to dansylation treatment to obtain a supernatant, and a sample for HPLC measurement was prepared. Samples were analyzed by HPLC (LC-30AD manufactured by Shimadzu Corporation).

A calibration curve was prepared using standard products of spermidine, spermine, and putrescine as the three types of polyamines, and the content of the three types of polyamines in the supernatant was determined. From that value, the elution amount (mg / 100 g) of the three types of polyamines into the aqueous solvent per 100 g of the treated product was determined. The results are shown in Table 1.

表１に示されるように、ハンマーミルを用いた比較例１、ホモミキサーを用いた比較例２、及び湿式超高圧粒子衝突型ホモジナイザーを用いた比較例３は、ともにポリアミンの溶出量の合計が２０ｍｇ／１００ｇ以下であった。

As shown in Table 1, Comparative Example 1 using a hammer mill, Comparative Example 2 using a homomixer, and Comparative Example 3 using a wet ultra-high pressure particle collision type homogenizer all have a total amount of polyamines eluted. It was 20 mg / 100 g or less.

On the other hand, in Examples 1 to 5 in which the pollen load was treated with cellulase or protease, it was confirmed that the total elution amount of polyamine was 25 mg / 100 g or more, which was significantly increased as compared with each Comparative Example. Further, in Example 4 in which the wet ultra-high pressure particle collision homogenizer was treated with the neutral protease and Example 5 in which the cellulase was treated with the neutral protease, the elution amount of spermidine was particularly significantly increased.

In Comparative Example 4 which was finally solid-liquid separated, spermidine was not detected, and it was confirmed that the elution amount of polyamine was significantly reduced as compared with each Example.

Claims (5)

A pollen-packed product obtained by treating a pollen load suspended in a solvent with at least one enzyme selected from cellulase and protease, and concentrating or drying the pollen load without solid-liquid separation. The pollen-packed product according to claim 1, wherein the enzyme is cellulase and a neutral protease. The pollen load-treated product according to claim 1 or 2, wherein the pollen load is further treated with a wet ultra-high pressure particle collision type homogenizer. A step of adding a solvent to a pollen load and treating it with at least one enzyme selected from cellulase and protease.
A method for treating pollen loads, which comprises a step of concentrating or drying without solid-liquid separation. The pollen load processed product obtained by treating the pollen load suspended in a solvent with at least one enzyme selected from cellulase and protease and concentrating or drying without solid-liquid separation is used as an active ingredient. The composition for anti-aging contained.