JP2021071455A - Sheet and method for collecting skin component - Google Patents

Abstract

To provide a sheet for collecting a skin component that allows a precise overview of the distribution of a skin component, and a method for collecting a skin component by using the sheet.SOLUTION: A horny cell layer collecting mask 5 includes: a sheet base material 8 over a collection target region, the material 8 having an adhesive surface in a first surface and having a non-adhesive surface in a second surface and also having a second sectional pattern 10 dividing a sheet-side section 11 related to each plural collection sections in the collection target region; and a separation sheet laminated on the adhesive surface of the sheet material 8.SELECTED DRAWING: Figure 4

Description

The present invention relates to a sheet for collecting skin components and a method for collecting skin components using the same.

Conventionally, the tape stripping method is mainly used as a method for collecting skin components in order to analyze the components contained in the skin.
For example, in Patent Document 1, in order to analyze and visualize the degree of penetration of the active ingredient contained in a transdermally absorbed preparation or the like into the stratum corneum, a human stratum corneum is subjected to a tape stripping operation using an adhesive sheet. It discloses a method of sequentially collecting (1st to 5th layers) from the outermost layer of the skin.

Further, Patent Document 2 discloses a method of stripping and collecting keratinocytes on the skin surface with cellophane tape from the cheek portion of the face and the medial portion of the upper arm in order to distinguish the skin characteristics such as sensitive skin.

Japanese Unexamined Patent Publication No. 2001-108674 Japanese Unexamined Patent Publication No. 2014-13201

Conventionally, a plurality of methods have been proposed in which an adhesive sheet such as cellophane tape is attached to the skin to collect skin components as in the methods of Patent Documents 1 and 2. However, in either method, the skin component is collected from a predetermined skin component collection target area, and the skin component contained in the collection target area is analyzed and verified for the entire site.
On the other hand, for example, each part of the body such as the cheeks, around the eyes, and around the mouth of the face, or the body is classified a little wider, and for example, a plurality of skin components contained in the entire face, arms, etc. are collected. No literature has been found that proposes an invention that can realize this by focusing on a bird's-eye view of the distribution of skin components in the collection target area such as the face based on the analysis results.

An object of the present invention is to provide a sheet for collecting skin components that can give an accurate bird's-eye view of the distribution of skin components, and a method for collecting skin components using the same.

The sheet for collecting skin components according to one aspect of the present invention is a sheet base material that covers a collection target area of skin components, and divides a sheet-side section associated with each of a plurality of collection portions in the collection target area. Includes a sheet substrate on which a compartment pattern is formed and which has an adhesive surface on one surface and a non-adhesive surface on the other surface, and a release sheet laminated on the adhesive surface of the sheet substrate.

The method for collecting a skin component according to another aspect of the present invention includes a step of peeling the release sheet from the sheet base material of the sheet for collecting the skin component, and after peeling the release sheet, the sheet base material. It includes a step of collecting skin components by attaching the adhesive surface to the skin and then removing the sheet substrate from the skin.

FIG. 1 is a flow chart of a method according to an embodiment of the present invention. FIG. 2 is a diagram showing a front face of a person for explaining a collection target area of the stratum corneum. FIG. 3 is a diagram showing a profile of a person for explaining a collection target area of the stratum corneum. FIG. 4 is a front view of a mask for collecting a stratum corneum according to an embodiment of the present invention. FIG. 5 is a rear view of a mask for collecting a stratum corneum according to an embodiment of the present invention. FIG. 6 is an enlarged cross-sectional view of a mask for collecting a stratum corneum according to an embodiment of the present invention. FIG. 7 is a diagram for explaining a process related to the collection of the stratum corneum. FIG. 8 is a diagram for explaining a process related to the collection of the stratum corneum. FIG. 9 is a diagram for explaining a process related to the collection of the stratum corneum. FIG. 10 is a diagram for explaining a process related to peeling of the stratum corneum from the sheet base material. FIG. 11 is a diagram for explaining a process related to staining and area measurement of the stratum corneum. FIG. 12 is a diagram for explaining a process related to visualization of the analysis result. FIG. 13 is a front view of a sheet for collecting a stratum corneum according to another embodiment of the present invention. FIG. 14 is a rear view of a sheet for collecting a stratum corneum according to another embodiment of the present invention. FIG. 15 is a diagram showing the visualization in three dimensions, and is a diagram showing an image of a three-dimensional image of the front face of a person. FIG. 16 is a diagram showing the visualization in three dimensions, and is a diagram showing an image of a three-dimensional image of a profile of a person.

<Embodiment of the present invention>
First, embodiments of the present invention will be listed and described.
The skin component collection sheet according to an embodiment of the present invention is a sheet base material that covers a skin component collection target area, and divides a sheet-side section associated with each of a plurality of collection portions in the collection target area. Includes a sheet substrate on which a compartment pattern is formed and which has an adhesive surface on one surface and a non-adhesive surface on the other surface, and a release sheet laminated on the adhesive surface of the sheet substrate.

This skin component collection sheet is subdivided in the collection target area by associating each sheet side section of the compartment pattern formed on the sheet base material with a plurality of collection portions in the skin component collection target area in advance. It is possible to independently analyze the skin components of each of the converted collected parts.
More specifically, for example, first, the adhesive surface of the sheet base material is attached to and peeled off from the collection target area composed of the aggregate of the collection portions. As a result, the skin component of the collection target area is collected on the adhesive surface of the sheet base material. Next, the sheet base material is classified by the sheet side section of the partition pattern. The skin components of each sheet-side section may be classified, for example, by cutting the sheet substrate along a compartment pattern, or by providing a partition at the boundary of adjacent sheet-side sections on the sheet substrate. It may be classified in other ways. The skin components of each classified sheet-side section are then analyzed individually. Next, by confirming the correspondence between each sheet-side section of the section pattern of the sheet base material and each collection portion of the skin collection target area, the skin components obtained from each sheet-side section of the sheet base material Based on the analysis result, the state of the skin component in each collected portion can be analyzed. Then, by collecting the analysis results of the obtained collected portion, it is possible to obtain an accurate bird's-eye view of the distribution of skin components in the area to be collected.

Then, by collecting this bird's-eye view result for a plurality of samples, for example, how the state of the skin component in the area to be collected of the skin changes before and after the use of cosmetics, according to age, gender, place of residence, etc. It can be verified whether it is.
In the sheet for collecting skin components according to the embodiment of the present invention, a mark that can be distinguished for each sheet side section may be formed in each sheet side section of the section pattern.

According to this configuration, each sheet-side section of the section pattern formed on the sheet base material and the collection portion of the skin component collection target area can be associated with each other by using a mark, so that the correspondence relationship can be easily made. You can check.
In the sheet for collecting skin components according to the embodiment of the present invention, the sheet base material may contain a transparent sheet base material.

According to this configuration, the sheet base material can be attached to the skin while confirming the positional relationship of the sheet base material with respect to the collection target area through the sheet base material. Therefore, the workability of collecting skin components can be improved.
In the sheet for collecting skin components according to one embodiment of the present invention, the compartment pattern may include a grid pattern.

According to this configuration, since a plurality of sheet-side sections are divided by grid-like lines and there is no gap between adjacent sheet-side sections, the state of skin components in the collection target area can be analyzed evenly.
In the sheet for collecting skin components according to the embodiment of the present invention, the compartment pattern is formed on the entire sheet base material from the central portion to the peripheral portion of the sheet base material, and is one of the sheet base materials. A notch may be formed in the portion from the peripheral edge of the sheet base material along the line of the grid pattern.

For example, the adjacent portions with the notch as a boundary are defined as the first portion and the second portion, respectively. Further, the first part is a collecting part used for collecting skin components, and the second part is a non-collecting part not used for collecting skin components. Since there is no problem even if skin components other than the collection target area are attached to the second part, the skin components can be collected by pinching the second part with a finger and attaching the sheet base material to the collection target area. .. At this time, by superimposing the second part (non-collecting part) on the first part (collecting part), the second part can be avoided from the collection target area, so that the collection of skin components is hindered by the second part. It can be prevented from being done.

In the sheet for collecting skin components according to one embodiment of the present invention, the release sheet may include a split release sheet that is divided and laminated in a plurality of portions with respect to the sheet base material.
According to this configuration, the portion where the release sheet has not been peeled off can be picked up with a finger, and the sheet base material can be attached to the collection target area while positioning the sheet base material with respect to the collection target area. As a result, the sheet base material can be accurately attached to the collection target area.

The skin component collecting sheet according to one embodiment of the present invention may include a facial skin component collecting mask.
The skin component collecting sheet according to the embodiment of the present invention may include a facial skin component collecting mask formed for the left half surface or the right half face of the face.
According to this configuration, by collecting the skin components on the left or right half of the face, the analysis result of the collection target half is adopted as the analysis result of the non-collection target half, so that the amount of work is smaller. The analysis of skin components of the entire face can be completed.

The method for collecting a skin component according to an embodiment of the present invention includes a step of peeling the release sheet from the sheet base material of the sheet for collecting the skin component, and after peeling the release sheet, the sheet base material. It includes a step of collecting skin components by attaching the adhesive surface to the skin and then removing the sheet substrate from the skin.
According to this method, since the above-mentioned sheet for collecting skin components is used, the distribution of skin components can be analyzed more accurately than in the past.
<Detailed Description of Embodiments of the Present Invention>
Next, an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

First, as an embodiment of the present invention, a method of collecting the horny layer of the face as an example of the collection target area and analyzing the area distribution of the horny layer on the face will be described below.
However, the present invention is not limited to the method of analyzing the area distribution of the stratum corneum, and an object of the present invention is to analyze the skin components collected by the adhesive sheet and to overlook the distribution of the analysis results of the skin components in the area to be collected. It is said. That is, the method for analyzing the area distribution of the stratum corneum described below is only an example of the present invention, and the present invention can be carried out in other forms.

For example, the collection area of the analysis sample is not limited to the face, but a relatively wide area of the skin can be targeted. For example, the arm, abdomen, chest, back, thigh, etc. may be the collection target area (collection target site).
Further, as the analysis sample, not only the stratum corneum but also all skin components can be widely adopted. The skin component may be a substance that is distributed on the surface layer of the skin regardless of whether the source is inside or outside the body. For example, substances secreted or synthesized from the body or the lower layer of the skin and distributed on the surface layer of the skin include the stratum corneum (including the components in the stratum corneum), intercellular lipids, lipids on the cell surface, sweat components, bacteria and the like. .. On the other hand, for example, as substances that are applied or adhered from outside the body and distributed on the surface layer of the skin, dust (on the skin), pollen, particulate matter (for example, PM2.5, etc.), components contained in tobacco smoke, etc. Examples include atmospheric substances.

Further, the purpose of analysis is not limited to the measurement of the distribution of the area of the stratum corneum, but as a broader concept, the measurement of the distribution of skin components in the area to be collected may be used. Other examples for specific analytical purposes include, for example, measurement of the lipid amount distribution in the cell surface layer, measurement of the nitration distribution in the stratum corneum, and the like.
Then, the method of collecting the stratum corneum of the face and analyzing the area distribution of the stratum corneum in the face will be described in detail below.

FIG. 1 is a flow chart of a method for analyzing the area of the stratum corneum according to the embodiment of the present invention.
In this method, first, the stratum corneum of the collection target area 2 is collected (step S1). In this embodiment, the collection target area 2 is the left half surface of the face, but of course, it may be the right half surface of the face.
Prior to the collection of the horny layer, in this embodiment, first, as shown in FIGS. 2 and 3, a plurality of collection sections 4 are set in the collection target area 2 of the target person 1 (the horny layer provider). As described above, the step of setting the collection section 4 can be omitted, of course, in light of the object of the present invention of overlooking the distribution of the analysis results of the skin components in the skin collection target region.

In this embodiment, a first compartment pattern 3 is set in which the entire collection target area 2 (left half surface of the face) is subdivided into a grid pattern in a plane, and each grid window portion of the grid-shaped first compartment pattern 3 is set. Allocate collection sections 4 one by one. Each sampling section 4 may be square or rectangular as shown in FIGS. 2 and 3. Further, in the case of a square shape, each collection section 4 may have a side of 1 cm to 2 cm.

For example, an image of the face of the photographed target person 1 is displayed on a computer monitor, and as shown in FIGS. 2 and 3, a virtual first division pattern in a grid pattern is displayed on the entire left half of the face. 3 may be overlapped, and each grid window portion of the grid-shaped first section pattern 3 may be set as a sampling section 4. At this time, each sheet-side section 11 of the second section pattern 10 of the mask 5 for collecting the stratum corneum, which will be described later, is associated with each collection section 4 on a one-to-one basis. For example, the same marks 12 (numbers in this embodiment) formed on each sheet side section 11 of the horny layer collecting mask 5 may be formed in each collecting section 4 and the correspondence relationship may be recorded.

Next, the mask 5 for collecting the stratum corneum shown in FIGS. 4 to 6 is prepared. The mask 5 for collecting the stratum corneum is formed in a shape that covers the collection target area 2, and in this embodiment, it is formed in a substantially semicircular shape that covers the left half surface of the face.
As shown in FIG. 6, the mask 5 for collecting the stratum corneum has a sheet base material 8 having an adhesive surface 6 on one surface and a non-adhesive surface 7 on the other surface, and a peeling layer laminated on the adhesive surface 6 of the sheet base material 8. Includes sheet 9 and. The release sheet 9 is removably laminated on the sheet base material 8 by being attached to the adhesive surface 6 of the sheet base material 8.

The sheet base material 8 may be, for example, one in which an adhesive is applied to one side of a sheet made of a synthetic resin such as polyethylene or polypropylene. Further, the sheet base material 8 may or may not have elasticity. Further, the sheet base material 8 is a transparent sheet base material 8 in this embodiment. Thereby, the sheet base material 8 can be attached to the skin while confirming the positional relationship of the sheet base material 8 with respect to the collection target area 2 (the left half surface of the face) through the sheet base material 8. Therefore, the workability of collecting the stratum corneum can be improved. Further, the sheet base material 8 is preferably, for example, having a thickness of 50 μm to 300 μm in consideration of sticking workability to the collection target area 2 having undulations.

The sheet base material 8 is formed with a second compartment pattern 10 for distinguishing the stratum corneum collected for each of the above-mentioned collection sections 4. The second section pattern 10 may be formed by a broken line or a solid line as shown in FIG. Further, the second section pattern 10 may be formed on the sheet base material 8 by printing, or may be perforations, irregularities formed on the sheet base material 8 by engraving or the like.

The second compartment pattern 10 is formed in a grid pattern. In this embodiment, the second compartment pattern 10 is formed over the entire sheet base material 8 from the central portion to the outer peripheral portion of the seat base material 8. As a result, the sheet base material 8 is formed with one sheet-side section 11 in each lattice window portion of the second section pattern 10.
Each sheet-side section 11 may have a square shape or a rectangular shape as shown in FIG. 4, in accordance with the above-mentioned collection section 4. Further, in the case of a square shape, each sheet side section 11 may have a side of 1 cm to 2 cm. In this embodiment, the seat side sections 11 of the second section pattern 10 are unified in the same size, but may be divided into a plurality of sizes such as small, medium, and large.

Further, each sheet side section 11 of the second section pattern 10 is not limited to a quadrangular shape, and may be, for example, a circular shape, a triangular shape, or the like. From the viewpoint that the stratum corneum of the collection target area 2 is evenly accommodated in one of the sheet-side sections 11, it is preferable to form it in a quadrangular shape by the grid-like second partition pattern 10. Further, the second section pattern 10 does not have to be in a grid pattern, and may be, for example, a pattern in which a plurality of sheet-side sections 11 are arranged in a dot (polka dot) shape or a staggered shape.

In each seat side section 11 of the second section pattern 10, a distinguishable mark 12 is formed for each seat side section 11. In this embodiment, different numbers are formed for each seat-side section 11, which distinguishes each seat-side section 11. Such a mark 12 does not have to be a number, and may be, for example, an alphabet (A, B, C ...), a combination of a number and an alphabet (1A, 2A, 3A ...), And the like. Further, the mark 12 may be formed on the sheet base material 8 by printing, or may be unevenness or the like formed on the sheet base material 8 by engraving or the like, as in the case of the second section pattern 10. Good.

Further, the sheet base material 8 may be formed with a margin 13 in which the second partition pattern 10 is not formed. For example, in FIG. 4, of the outer peripheral edge portion of the sheet base material 8, a margin 13 is formed on the outer peripheral edge portion to be attached along the center line of the face. If the margin 13 is formed on the outer peripheral edge portion, the margin 13 can be picked up with a finger when the sheet base material 8 is attached to the collection target area 2, so that the attachment workability can be improved.

The release sheet 9 may be, for example, one in which a release agent is applied to one side of a sheet made of paper or synthetic resin. In this embodiment, the release sheet 9 is a split release sheet 9 which is divided into a plurality of portions and laminated with respect to the sheet base material 8. More specifically, as shown in FIG. 5, the dividing line 14 is formed in the vertical direction of the sheet base material 8 (the direction along the outer peripheral edge portion attached along the center line of the face), and the dividing line 14 is formed. The left half surface and the right half surface can be separated independently with the line 14 as a boundary. In this embodiment, the release sheet 9 includes a first portion 15 on the center line side of the face with respect to the dividing line 14, and a second portion 16 on the opposite side.

Further, a plurality of notches 17 and 18 are formed in the mask 5 for collecting the stratum corneum. The plurality of notches 17 and 18 may be formed in a laminated structure of the sheet base material 8 and the release sheet 9 as shown in FIGS. 4 and 5, or may be formed only in the sheet base material 8. The plurality of notches 17, 18 include, for example, a first notch 17 and a second notch 18.
The first notch 17 is formed in a straight line or a curved shape that is bent in the middle, starting from the outer peripheral edge 19 of the mask 5 for collecting the stratum corneum. For example, when the collection target area 2 has a relatively large convex portion, the first notch 17 may have a shape formed along the outer shape of the convex portion. In this embodiment, the first notch 17 is formed along the outer shape of the nose, which is a convex portion of the face.

As a result, the sheet base material 8 is formed with a swinging portion 20 having a shape that follows the outer shape of the convex portion of the collection target region 2. A part of the swinging portion 20 is cantilevered and supported by the remaining portion of the sheet base material 8 (the portion where the first notch 17 is not formed), and can swing in the thickness direction of the sheet base material 8. It has become. Therefore, when the sheet base material 8 is attached to the collection target area 2 (in this embodiment, the left half of the face), the rocking portion 20 in contact with the convex portion (in this embodiment, the nose) of the collection target area 2. Can be released, so that the tension of the sheet base material 8 can be suppressed.

The second notch 18 is formed in a straight line starting from the outer peripheral edge 19 of the mask 5 for collecting the stratum corneum. In this embodiment, a plurality of second notches 18 are formed from the outer peripheral edge 19 of the sheet base material 8 along the lines 21 of the grid-like second partition pattern 10. As a result, the sheet base material 8 has the first portions 22, 24, 26 and the second portions 23, 25, 27 composed of a plurality of continuous sheet-side sections 11 adjacent to each other with the second notch 18 as a boundary. It is formed. In FIG. 4, the first part 22, 24, 26 and the second part 23, 25, 27 are shown in gray for clarity.

For example, a second portion 23 composed of seat side sections 11 of 43 to 46 is formed with respect to a first portion 22 composed of seat side sections 11 of 34 to 36. Further, a second portion 25 composed of the seat side sections 11 of 96 to 99 is formed with respect to the first portion 24 composed of the seat side sections 11 of the 85th to 88th. Further, a second portion 27 composed of the seat side sections 11 of 133, 142, 150 and 157 is formed with respect to the first portion 26 composed of the seat side sections 11 of 134, 143, 151 and 158.

The second notch 18 is formed so as to reach the dividing line 14 from the outer peripheral edge 19 of the sheet base material 8. As a result, the second portion 16 of the sheet base material 8 and the release sheet 9 is divided into upper portions 28, 31, middle portions 29, 32, and lower portions 30, 33. In the upper portions 28, 31, the middle portions 29, 32, and the lower portions 30, 33, in this embodiment, the sheets for collecting the stratum corneum are masks, and the upper portions 28, 31, and the middle portions are arranged vertically. They are only referred to as parts 29,32 and lower parts 30,33. That is, the sheet base material 8 and the release sheet 9 divided by the second notch 18 may be referred to as a third portion, a fourth portion, a fifth portion, or the like, respectively, regardless of the alignment direction with each other.

The above-mentioned mask 5 for collecting the stratum corneum can be produced, for example, by printing the second section pattern 10 and the mark 12 on a sticker sheet that can be printed by an inkjet printer, a laser printer, or the like, and cutting the mask into a desired mask shape. .. The first notch 17 and the second notch 18 may be formed after being cut into a mask shape.
Then, in order to collect the stratum corneum using the above-mentioned mask 5 for collecting the stratum corneum, for example, first, a part of the divided release sheet 9 is peeled off from the sheet base material 8, and the adhesive surface of the sheet base material 8 is peeled off. A part of 6 is exposed. In this embodiment, as shown in FIG. 7, the first portion 15 (center line side of the face) of the release sheet 9 is peeled off. Next, the margin 13 of the sheet base material 8 and the portion where the release sheet 9 of the sheet base material 8 remains are pinched with fingers, for example, while looking in a mirror and checking the position of the nose through the transparent sheet base material 8. , The portion of the sheet base material 8 below the rocking portion 20 is aligned with the lower end of the nose and attached. From that state, the swinging portion 20 is attached to the central portion of the nose, and the remaining portion of the sheet base material 8 is attached to the forehead, around the eyes, around the mouth, and around the chin.

Regarding the parts where the load is applied to the target person 1 (subject) such as around the eyes and around the mouth, the load may be reduced by the following measures (1) to (3).
(1) The mask 5 may have an opening formed in advance in a portion corresponding to the relevant portion such as around the eyes or around the mouth.
(2) In order to reduce the burden on the relevant parts such as around the eyes and around the mouth, the adhesive strength of the parts in contact with the relevant parts may be weakened.
(3) The mask 5 may be attached after protecting the relevant parts such as around the eyes and around the mouth with cotton or the like.

As described above, since the release sheet 9 is the split release sheet 9, the portion where the release sheet 9 is not peeled is picked up with a finger, and the sheet base material 8 is positioned with respect to the collection target area 2 while the sheet base material 8 is positioned. 8 can be pasted on the collection target area 2. Therefore, the sheet base material 8 can be accurately attached to the collection target area 2.
Next, the remaining portion of the divided release sheet 9 is peeled from the sheet base material 8, and the remaining portion of the adhesive surface 6 of the sheet base material 8 is exposed. In this embodiment, the upper portion 31, the middle portion 32, and the lower portion 33 of the release sheet 9 are peeled off in order, and the parts 28, 29, and 30 of the sheet base material 8 from which the release sheet 9 has been peeled off are first attached to the face. I will go. First, as shown in FIG. 8, by peeling the upper portion 31 of the release sheet 9, the upper portion 28 of the sheet base material 8 including the first portion 22 of the pair of first portion 22 and the second portion 23 is removed. Expose.

Then, the upper portion 28 of the sheet base material 8 is attached around the eyes. Next, by peeling the middle portion 32 of the release sheet 9, the middle portion 29 of the sheet base material 8 including the second portion 23 and the first portion 24 of the pair of the first portion 24 and the second portion 25. To expose. Then, the second portion 23 is picked up with fingers, and the middle portion 29 of the sheet base material 8 is attached around the eyes and on the cheeks so that the second portion 23 is overlapped on the first portion 22.

As described above, the first portion 22 adjacent to the second notch 18 as a boundary is used as a collecting portion used for collecting the stratum corneum, and the second portion 23 is used as a non-collecting portion not used for collecting the stratum corneum. Since there is no problem even if a horny layer other than the collection target area 2 (for example, the horny layer of the finger) is attached to the second part 23, the second part 23 is picked with a finger and the sheet base material 8 is collected. The stratum corneum can be collected by pasting on 2.

At this time, by superimposing the second part 23 (non-collecting part) on the first part 22 (collecting part), the second part 23 can be avoided from the collection target area 2, so that the horny layer can be collected first. It can be prevented from being hindered by the two parts 23. That is, since the second portion 23 overlaps the first portion 22, the seat side sections 11 of 53 to 55 can be adjacent to the seat side sections 11 of 34 to 36 (see FIG. 4). Therefore, the stratum corneum of the facial portion adjacent to the sheet-side section 11 of Nos. 34 to 36 can be reliably collected.

Next, as shown in FIG. 9, the sheet group including the second portion 25 and the first portion 26 of the pair of the first portion 26 and the second portion 27 by peeling the lower portion 33 of the release sheet 9. The lower portion 30 of the material 8 is exposed. Then, the second portion 25 is picked up with fingers, and the lower portion 30 of the sheet base material 8 is attached around the cheeks and chin so that the second portion 25 is overlapped on the first portion 24. Then, for example, the adhesiveness of the sheet base material 8 to the skin may be increased by pressing the entire sheet base material 8 against the face.

Then, by peeling the sheet base material 8 from the face, the collection of the stratum corneum of the face is completed. At this time, if the margin 13 of the sheet base material 8 is picked and peeled off with a finger, it is possible to prevent extra skin components (for example, the stratum corneum of the finger) from adhering to each sheet side section 11 of the sheet base material 8. be able to.
Next, the sheet base material 8 from which the stratum corneum has been collected is classified into a desired size (for each section 11 on the sheet side) (step S2 in FIG. 1). In this embodiment, the sheet base material 8 is divided along the line 21 of the second section pattern 10, and each sheet side section 11 is separated. The sheet base material 8 can be separated by a cutting tool such as scissors or a cutter knife.

Next, as shown in FIG. 10, the sheet base material 8 for each of the cut sheet-side sections 11 is housed in an independent container 34 (for example, a heat-resistant container), and the peeling / dispersion solution 35 is contained in the container 34. (Step S3). As the stripping / dispersion solution 35, for example, a mixed solution of SDS 2%, EDTA 5 mM, Tris-HCl 0.1 M buffer (pH 8.5), and DTT 20 mM can be used. Then, for example, the container 34 is sealed with a heat-resistant seal or the like, and the peeling / dispersion solution 35 is boiled for, for example, 1 minute to 10 minutes. As a result, the stratum corneum adhering to the sheet base material 8 is peeled off from the sheet base material 8.

Then, the container 34 is left to about room temperature, and the heat-resistant seal is removed. Then, the liquid portion of the solution is collected in a centrifugable container while avoiding the sheet base material 8 and the adhesive peeled off from the sheet base material 8 as much as possible. The collected solution is then centrifuged to precipitate the stratum corneum and remove the supernatant of the solution. As a result, the stratum corneum is recovered (step S4).

Next, the precipitate containing the stratum corneum produced by centrifugation in each container is transferred to, for example, a predetermined container (for example, each well of the microplate). The solvent in each well is then removed by drying. For example, the solvent may be removed by leaving the microplate in an incubator set at 60 ° C. for 5 to 6 hours. After drying, a fixative (eg, formalin solution) is added to fix the stratum corneum in the wells (step S5).

Next, the fixative is removed, large dust and the like are removed by washing, and then the stratum corneum is stained with the staining solution (step S6). As the staining solution, for example, a Gentian violet solution or the like can be used, but the staining solution is not limited to this. After staining, excess staining solution is removed by washing with water multiple times.
Next, an image of the stratum corneum 36 is acquired by, for example, photographing the stratum corneum with a microscope (step S7). Then, by processing the obtained image with a computer, the shape of the stratum corneum 36 collected in each sheet side section 11 is analyzed as shown in FIG. 11 (step S8). For example, FIG. 11 shows an image of the stratum corneum 36 taken in each of the sheet-side sections 11 of the sheet base material 8, Nos. 5, 9, and 12.

Next, based on the analysis data of the stratum corneum 36 obtained above, the average area of the stratum corneum 36 included in each sheet side section 11 is calculated (step S9). Next, the calculated area of the stratum corneum 36 is classified into a plurality of area categories (step S10). The number of area divisions is not particularly limited as long as it is plural, but it is preferable to classify them into, for example, 3 to 5 divisions so that they can be easily distinguished from each other in the visualization described later.

The steps shown in steps S3 to S9 can be omitted by using, for example, computer technology such as artificial intelligence (AI). That is, the area of the stratum corneum may be calculated without dispersing and dyeing the stratum corneum collected from the sheet substrate 8. For example, even if the stratum corneum collected from the sheet base material 8 overlaps with each other, by using artificial intelligence technology, the remaining size can be grasped from the size and shape of the stratum corneum in the non-overlapping portion. The area of the collected stratum corneum can be calculated.

Next, as shown in FIG. 12, a map 39 imitating a human face is displayed on the monitor 38 of the computer 37, and colors corresponding to each area division are displayed on the map 39 (step S11). This map 39 reflects a grid-like partition pattern 40 that matches the first partition pattern 3 of the collection target area 2. That is, the stratum corneum included in each sheet-side section 11 is the collection section 4 of the collection target area 2 based on the association between each sheet-side section 11 of the sheet base material 8 and each collection section 4 of the collection target area 2. It is determined whether the sample is taken from the map 39, and the area classification is reflected in the corresponding section of the map 39. In FIG. 12, for convenience, the magnitude relationship of the area division is shown by hatching or shading of different patterns instead of the color.

As a result, the area distribution of the stratum corneum is heat-mapped on the monitor 38. Therefore, by looking down at the map 39 of the monitor 38, what is the area of the stratum corneum in the entire face of the target person 1 (the stratum corneum provider)? You can see if it is distributed like this.
In this embodiment, only the area distribution of the stratum corneum on the left half of the face is heat-mapped on the monitor 38, but the area distribution of the stratum corneum is considered to be generally symmetrical with respect to the center line of the face. , The area distribution of the stratum corneum on the right half surface may be heat-mapped based on the obtained data on the left half surface. That is, by calculating the area of the stratum corneum of the face collection target half surface (in this embodiment, the left half surface), the analysis result of the face collection target half surface is adopted as the analysis result of the non-collection target half surface, and the non-collection target half surface is adopted. Since the area of the stratum corneum (on the right half surface in this embodiment) can also be calculated, the area distribution of the stratum corneum of the entire face can be analyzed with a smaller amount of work.

According to the above method, the area of the stratum corneum is calculated for each of the plurality of sampling sections 4 in the sampling target area 2, then each calculation result is aggregated, and the area distribution of the stratum corneum is heat-mapped on the monitor 38. .. As a result, as described above, by looking down on the map 39 of the monitor 38, it is possible to know how the area of the stratum corneum is distributed over the entire face of the target person 1 (the stratum corneum provider). it can.

As a result, for example, for the target person 1 (the stratum corneum provider), the distribution of the stratum corneum over the entire face can be known, and for the skin care counselor, the customer at the salon, storefront, etc., based on the map 39. (Target person 1) can be given skin counseling. Further, for example, for a researcher, the distribution of the horny layer of the face of the target person 1 (the horny layer provider) can be grasped from the researcher's point of view, and a plurality of maps 39 displaying the area distribution of the horny layer of each person are displayed. By collecting the target persons (plural samples), for example, how the area of the stratum corneum in the collection target area 2 changes before and after the use of cosmetics, according to age, gender, place of residence, etc. It can also be verified.

Further, since the collection target area 2 is subdivided by the first section pattern 3 and the area of the stratum corneum included in each sampling section 4 of the collection target region 2 can be calculated independently, the area of the stratum corneum in the collection target region 2 can be calculated independently. The area distribution can be analyzed accurately. Further, in calculating the area of the stratum corneum, the stratum corneum is peeled from the sheet base material 8 in the peeling / dispersion solution 35 (see FIG. 10), and then the area of the dyed stratum corneum is used as the calculated value. The area of the stratum corneum for each section 4 can also be calculated accurately.

In this embodiment, a plurality of collection sections 4 are set in the collection target area 2, and by associating with each sheet side section 11 of the sheet base material 8, the collection position of the stratum corneum collected in each sheet side section 11 Is specified. However, even if the collection section 4 is not set, collection is performed in each sheet side section 11 based on the position of each sheet side section 11 on the sheet base material 8 and the attachment position of the sheet base material 8 with respect to the collection target area 2. It is also possible to roughly infer from which part of the collection target area 2 the collected stratum corneum was collected.

Further, since the area of the stratum corneum for each sampling section 4 is grouped according to the area classification and visualized in the section pattern 40 of the map 39, the rough size relationship of the area of the stratum corneum in the sampling target area 2 can be easily understood. Can be grasped. Moreover, since each area division is visualized in different colors, it is possible to easily grasp the magnitude relationship of the area of the stratum corneum in the collection target area 2 just by looking at the visualized area division. Further, by visualizing using the computer 37, various colors having different hue, lightness, and saturation can be used in the color display of the area division.

Further, since the plurality of sheet-side sections 11 are partitioned by the second section pattern 10 composed of grid-like lines and there is no gap between the adjacent sheet-side sections 11, the area of the stratum corneum in the collection target area 2 is analyzed evenly. can do. Further, by aligning the sizes of the sheet-side sections 11 in a matrix, the area of the stratum corneum between the sheet-side sections 11 can be easily compared.

Then, the analysis of the area distribution of the stratum corneum as described above can be performed more efficiently by using the mask 5 for collecting the stratum corneum shown in FIGS. 4 to 6.
That is, by using the mask 5 for collecting the stratum corneum, the area of the stratum corneum of each sheet side section 11 of the sheet base material 8 can be calculated individually. Then, by confirming the correspondence between each sheet side section 11 of the second section pattern 10 of the sheet base material 8 and each collection section 4 of the collection target area 2, from each sheet side section 11 of the sheet base material 8. Based on the obtained calculation result of the area of the stratum corneum, the area of the stratum corneum in each sampling section 4 can be easily analyzed. Then, by collecting the obtained analysis results of the collection section 4, the area distribution of the stratum corneum in the collection target area 2 can be accurately determined.

Further, since the mark 12 is formed on the sheet base material 8 of the mask 5 for collecting the stratum corneum, each sheet side section 11 of the second section pattern 10 of the sheet base material 8 and the collection set in the collection target area 2 are collected. Since the section 4 can be associated with the mark 12 by using the mark 12, the correspondence can be easily confirmed.
Although the embodiments of the present invention have been described above, the present invention can also be implemented in other embodiments.

As described above, the present invention is not limited to the method of collecting the stratum corneum of the face and analyzing the area distribution of the stratum corneum in the face, and the mask 5 for collecting the stratum corneum used in the method.
For example, the above-mentioned mask for collecting the stratum corneum 5 can also be used as a mask for collecting skin components for collecting skin components other than the stratum corneum of the face. When the collection target area 2 is other than the face, for example, as shown in FIGS. 13 and 14, the skin component collection sheet 41 in which the sheet base material 8 and the release sheet 9 are laminated may be used. In FIGS. 13 and 14, a rectangular skin component collecting sheet 41 is shown as an example. In this case as well, the second compartment pattern 10 may be formed on the sheet base material 8 as in the case of the mask 5 for collecting the stratum corneum. Such a skin component collecting sheet 41 can be freely used for analysis of skin components in various parts of the skin. Of course, it may be used on a part of the face (for example, forehead, cheeks, etc.).

Further, as shown in FIGS. 15 and 16, the map 39 in which the distribution of skin components is visualized can be represented three-dimensionally. To visualize the distribution of skin components in three dimensions, for example, a three-dimensional image 42 of the face of the target person 1 is acquired by a three-dimensional digitizer, and the three-dimensional image 42 and the information of the above-mentioned map 39 are computerized. It may be combined on (for example, three-dimensional CAD software). Examples of the device used for this operation include "VIVID910" manufactured by Konica Minolta, "VECTRA M3" manufactured by Canfield Scientific, and "GO! SCAN" manufactured by Creaform as non-contact 3D digitizers. Examples of the three-dimensional CAD software include "SolidWorks" manufactured by Dassault Systemes, "Creo Parametric 3D" manufactured by PTC, and "Inventor" manufactured by AUTODESK.

This makes it possible to grasp the distribution of skin components in three dimensions. In addition, in FIG. 15 and FIG. 16, as an example, the distribution of the stratum corneum area shown in FIG. 12 is shown as a three-dimensional image 42. In addition, in FIG. 15 and FIG. 16, the three-dimensional image 42 of the face of the target person 1 is shown as a diagram for clarity.
Further, an example of an analysis method for an analysis purpose other than the measurement of the distribution of the area of the stratum corneum is as follows.

For example, when measuring the lipid amount distribution on the cell surface, the pressure-sensitive adhesive sheet after being adhered to the collection target area of the skin is immersed in a solvent capable of dissolving the target lipid such as diethyl ether for extraction, and then gas chromatography is performed. Just measure.
In addition, when measuring the nitration distribution of the stratum corneum, the adhesive sheet after being adhered to the collection target area of the skin can be observed by antibody staining with a nitrated tyrosine antibody, for example, PBS (-). ), Treated with an ultrasonic crusher, and then quantified with a commercially available nitrated tyrosine quantification kit.

That is, by heat-mapping the amount of lipid on the cell surface and the quantitative value of nitration obtained according to the above method and collecting the heat map for a plurality of target persons (plurality of samples), for example, the lipid in the collection target region 2 It is possible to verify how the amount distribution and nitration are related to age, gender, place of residence, etc.
Further, in the above-mentioned analysis method of the area of the stratum corneum, the stratum corneum of the sampling target area 2 is collectively sampled using one mask 5 for collecting the stratum corneum, but a separate sheet or the like is used for each sampling section 4. You may collect the stratum corneum with. For example, the stratum corneum of each collection section 4 may be collected by attaching one adhesive sticker or the like to each collection section 4, and each adhesive seal may be dispersed as it is in the peeling / dispersion solution 35 without being divided. .. In this case, the workability of attaching the sheet is lowered, but the workability is improved in this respect because the sheet does not have to be divided for each sampling section 4. Even with such a method, the distribution of the area of the stratum corneum in the collection target area 2 can be overlooked by collecting the analysis results of the components collected from each sheet.

Further, if the correspondence between each sampling section 4 and each sheet side section 11 can be recorded, it is not necessary to form the mark 12 on the sheet base material 8.
In addition, various design changes can be made within the scope of the matters described in the claims.

1 Target person 2 Collection target area 3 1st section pattern 4 Collection section 5 Mask for collecting stratum corneum 6 Adhesive surface 7 Non-adhesive surface 8 Sheet base material 9 Peeling sheet 10 2nd section pattern 11 Sheet side section 12 Mark 14 Dividing line 15 (Peeling sheet) 1st part 16 (Peeling sheet) 2nd part 17 1st notch 18 2nd notch 19 (Mask) outer peripheral edge 21 (2nd section pattern) Line 22 (Sheet base material) 1st part 23 (Sheet) Base material) 2nd part 24 (Sheet base material) 1st part 25 (Sheet base material) 2nd part 26 (Sheet base material) 1st part 27 (Sheet base material) 2nd part 36 Horn layer 41 For collecting skin components Sheet 42 3D image

Claims (9)

A sheet base material that covers a collection target area of a skin component, in which a compartment pattern for partitioning a sheet-side section associated with each of a plurality of collection portions in the collection target area is formed, and an adhesive surface and another surface are formed on one surface. With a sheet base material that has a non-adhesive surface,
A sheet for collecting skin components, which comprises a release sheet laminated on the adhesive surface of the sheet base material. The sheet for collecting skin components according to claim 1, wherein a distinguishable mark is formed for each sheet-side section of the section pattern. The sheet for collecting skin components according to claim 1 or 2, wherein the sheet base material contains a transparent sheet base material. The sheet for collecting skin components according to any one of claims 1 to 3, wherein the compartment pattern includes a grid pattern. The partition pattern is formed on the entire sheet base material from the central portion to the peripheral portion of the sheet base material.
The sheet for collecting skin components according to claim 4, wherein a notch is formed in a part of the sheet base material along the line of the grid pattern from the peripheral edge of the sheet base material. The sheet for collecting skin components according to any one of claims 1 to 5, wherein the release sheet includes a divided release sheet that is divided into a plurality of portions and laminated on the sheet base material. The sheet for collecting skin components according to any one of claims 1 to 6, which comprises a mask for collecting facial skin components. The sheet for collecting skin components according to claim 7, which comprises a mask for collecting skin components of the face formed for the left half surface or the right half face of the face. A step of peeling the release sheet from the sheet base material of the sheet for collecting skin components according to any one of claims 1 to 8.
A method for collecting a skin component, which comprises a step of collecting the skin component by attaching the adhesive surface of the sheet base material to the skin after peeling the release sheet and then removing the sheet base material from the skin.

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