JP2021065225A5

JP2021065225A5 -

Info

Publication number: JP2021065225A5
Application number: JP2020153761A
Authority: JP
Filing date: 2020-09-14
Publication date: 2023-02-06

Description

第１切替器６１２、第２切替器６１４および第３切替器６１６を操作することで回収装置１ｂに形成される流路を第１流路、第２流路、または第３流路に切り替えることができる。 By operating the first switching device 612, the second switching device 614, and the third switching device 616, the flow path formed in the recovery device 1b is switched to the first flow path, the second flow path, or the third flow path. be able to.

実施形態３にかかる回収装置１ｂを用いた微生物回収方法は、洗浄するステップＳ１２０にかえて、洗浄ステップＳ１２０’を備える点で実施形態２にかかる回収装置１ａを用いた微生物回収方法（図５参照）と異なる。 The microorganism collection method using the collection device 1b according to the third embodiment includes a washing step S120′ in place of the washing step S120. ) is different.

実施形態１～実施形態３においては、廃液容器４２０内を減圧することで、微生物３４０を濾過器２００を用いて濾別したり、液体試料３００ａを第１フィルタ１４０および第２フィルタ１６０で濾過したり、あるいは、洗浄液３８０を濾過器２００内に流す例を示した。なお、液体を回収装置１，１ａ，１ｂ内に流す方法は、出口側（二次側）を減圧する方法に限られず、たとえば、入り口側（一次側）を加圧することで実現してもよく、また、出口側（二次側）を減圧し、かつ入り口側（一次側）を加圧することで実現してもよい。 In Embodiments 1 to 3, by reducing the pressure in the waste liquid container 420, the microorganisms 340 are filtered using the filter 200, and the liquid sample 300a is filtered using the first filter 140 and the second filter 160. Alternatively, an example of flowing the cleaning liquid 380 into the filter 200 is shown. The method of flowing the liquid into the recovery devices 1, 1a, and 1b is not limited to the method of reducing the pressure on the outlet side ( secondary side). Alternatively, the outlet side (secondary side) may be depressurized and the inlet side (primary side) may be pressurized.

なお、回収路５１８と流入口２０２との合流点（第１コネクタ７２０との接続点）を洗浄路５２０と流入口２０２との合流点（第１コネクタ７２０との接続点）よりも濾過器２００側に位置するようにしてもよい。このように構成することで、液体試料３００ａ（血球除去後の液体試料３００ａ）が通った流路のうち、少なくとも洗浄液３８０が通った流路を培地３６０が通過することになるため、培地３６０へのコンタミネーションの発生を防止できる。 Note that the confluence of the collection path 518 and the inlet 202 (connection point with the first connector 720) is positioned closer to the filter 200 than the confluence of the cleaning path 520 and the inlet 202 (connection point with the first connector 720). It may be located on the side. By configuring in this way, the medium 360 passes through at least the flow path through which the washing liquid 380 passed, among the flow paths through which the liquid sample 300a (the liquid sample 300a after removal of blood cells) passed. contamination can be prevented.

以上のように、実施形態４にかかる回収装置１ｃは、第１切替器６１２ａ，６１２ｂ，６１２ｃ、および第２切替器６１４ａ，６１４ｂのそれぞれが挟み込んでいる管を圧迫したり、あるいは、圧迫を解放したりすることで、第１流路、第２流路、第３流路の切り替えを行う。 As described above, the recovery device 1c according to the fourth embodiment compresses or releases the tube sandwiched by the first switches 612a, 612b, 612c and the second switches 614a, 614b. By doing so, switching among the first flow path, the second flow path, and the third flow path is performed.

記憶媒体は、ＤＶＤ-ＲＯＭ（Digital Versatile Disk Read Only Memory）、ＣＤ－ＲＯＭ（compact disc read-only memory）、ＦＤ（Flexible Disk）、ハードディスクに限られず、磁気テープ、カセットテープ、光ディスク（ＭＯ（Magneto Optical Disc）／ＭＤ（Mini Disc）／ＤＶＤ（Digital Versatile Disc））、光カード、マスクＲＯＭ、ＥＰＲＯＭ（Electronically Programmable Read-Only Memory）、ＥＥＰＲＯＭ（Electronically Erasable Programmable Read-Only Memory）、フラッシュＲＯＭなどの半導体メモリなどの固定的にプログラムを担持する媒体としてもよい。また、記録媒体は、プログラムなどをコンピュータが読取可能な非一時的な媒体である。 The storage medium is not limited to DVD-ROM (Digital Versatile Disk Read Only Memory), CD-ROM (compact disc read-only memory), FD (Flexible Disk), hard disk, magnetic tape, cassette tape, optical disk (MO (Magnet o Optical Disc) / MD (Mini Disc) / DVD (Digital Versatile Disc)), optical cards, mask ROM, EPROM (Electronically Programmable Read-Only Memory), EEPROM (Electronically Erasable Programmable Read-Only Memory), flash ROM, etc. A medium such as a semiconductor memory that holds the program fixedly may be used. Also, the recording medium is a computer-readable non-transitory medium for programs and the like.

（第１２項）第９項または第１０項に記載の微生物回収装置は、洗浄液を受ける洗浄容器と、濾過器の第１開口部側に洗浄容器が接続され、濾過器の第２開口部側に廃液容器が接続される第３流路をさらに備えてもよい。 (Item 12) The microorganism recovery apparatus according to item 9 or 10 includes a cleaning container for receiving a cleaning liquid, a cleaning container connected to the first opening side of the filter, and a second opening side of the filter. may further include a third channel to which the waste liquid container is connected.

Claims

filtering microorganisms from a liquid sample using a filter;
The recovery liquid is allowed to flow from the filtrate discharge side of the filter toward the liquid sample inflow side of the filter, thereby recovering the microorganisms separated by the filter together with the recovery liquid. A method for recovering microorganisms, comprising:

2. The method for recovering microorganisms according to claim 1, wherein said recovery liquid is a liquid medium for use in culturing said microorganisms.

The step of recovering the microorganisms together with the recovery liquid comprises: connecting a channel connected to the liquid sample inflow side of the filter to a recovery container for recovering the recovery liquid containing the microorganisms; 3. The microorganism recovery method of claim 1 or claim 2, comprising the step of switching to a channel.

further comprising flowing a washing liquid from a side of the filter into which the liquid sample is introduced toward a side of the filter from which the filtrate is discharged;
4. The method according to any one of claims 1 to 3, wherein the step of flowing the washing liquid is performed between the step of filtering the microorganisms and the step of collecting the microorganisms together with the collection liquid. Microbial recovery method.

5. The step of flowing the cleaning liquid according to claim 4, wherein the step of flowing the cleaning liquid includes switching a channel connected to the liquid sample inflow side of the filter to a cleaning channel connected to a cleaning container for receiving the cleaning liquid. Microbial recovery method.

the liquid sample contains blood cells,
further comprising removing the blood cells from the liquid sample;
The microorganism recovery method according to any one of claims 1 to 5, wherein the step of removing blood cells is performed before the step of filtering out the microorganisms.

The step of removing blood cells includes:
performing a first filtration on the liquid sample using a first filter having a lower filtration resistance to the microorganisms than to the blood cells, thereby causing the microorganisms to precede the blood cells;
performing a second filtration using a second filter on the filtrate containing the microorganisms while the blood cells remain in the first filter to size-selectively permeate the microorganisms;
7. The steps of removing the blood cells and filtering out the microorganisms are performed by at least one of pressurization from the primary side of the first filtration and decompression from the side from which the filtrate is discharged. The method for recovering microorganisms according to .

The step of recovering the microorganisms together with the recovery liquid includes switching a channel connected to a side of the filter from which the filtrate is discharged to a channel connected to a recovery liquid container for receiving the recovery liquid. The method for recovering microorganisms according to any one of claims 1 to 7, comprising steps.

a sample container for receiving a liquid sample;
a filter having a pore size smaller than that of the microorganisms, and a filter having a first opening and a second opening formed at positions facing each other with the filter sandwiched therebetween;
a waste container for receiving waste liquid;
a recovery liquid container for receiving the recovery liquid;
a collection container for receiving a solution to be collected;
a first channel to which the sample container is connected to the first opening side of the filter, and to which the waste liquid container is connected to the second opening side of the filter;
and a second channel to which the recovery liquid container is connected to the second opening side of the filter, and a second flow path to which the recovery container is connected to the first opening side of the filter.

10. The apparatus for recovering microorganisms according to claim 9, wherein said recovery liquid is a liquid medium for culturing said microorganisms.

a first switcher for switching connection with the first opening to the sample container or the collection container;
11. The microorganism recovery apparatus according to claim 9, further comprising a second switching device that switches connection with the second opening to the waste liquid container or the recovery liquid container.

a cleaning container for receiving the cleaning fluid;
11. Further comprising a third channel to which the washing container is connected to the first opening side of the filter, and to which the waste liquid container is connected to the second opening side of the filter. Microorganism recovery device according to.

a first switcher for switching connection with the first opening to the sample container, the collection container, or the washing container;
13. The microorganism recovery apparatus according to claim 12, further comprising a second switching device that switches connection with the second opening to the waste liquid container or the recovery liquid container.

the liquid sample contains blood cells,
The sample container is
A removal device for removing the blood cells,
a first filter that is permeable to the blood cells and the microorganisms that are smaller than the blood cells and that has a property of allowing the microorganisms to permeate before the blood cells;
and a second filter arranged downstream of the first filter, capable of size-selectively capturing the blood cells and size-selectively permeating the microorganisms. The microorganism collection device according to any one of the items.

a housing that accommodates the sample container and the filter and detachably supports the collection container;
Further comprising a container in which the first switching device and the second switching device are arranged and configured to be able to attach the housing,
The housing is provided with at least part of the first flow path and at least part of the second flow path,
The first flow path and the second flow path provided in the housing are configured by a tube having plasticity,
The first switching device and the second switching device sandwich and press the tube to block the first flow path or the second flow path and switch connection,
The first switch and the second switch are positioned in the container such that the pipe is sandwiched between the first switch and the second switch when the housing is attached to the container. 14. The microorganism recovery device according to claim 11 or claim 13, wherein