JP2021035373A - Powdery mildew-resistant genes in carrot - Google Patents

Abstract

To provide carrot plants or Daucus carota plant resistant to powdery mildew, especially powdery mildew caused by the plant pathogen Erysiphe heraclei where the powdery mildew resistance is provided by one or two dominant powdery mildew resistance genes, to provide molecular markers genetically linked to the powdery mildew resistance-providing genes and the use thereof for identifying carrot plants being resistant to the powdery mildew, and to provide seeds, edible plant parts, pollen, egg cells, callus, suspension culture, somatic embryos or plant parts of the plant of the invention.SOLUTION: Disclosed is a Daucus carota plant resistant to powdery mildew caused by a plant pathogen Erysiphe heraclei, whose resistance is provided by a first resistance gene located between specific sequences on chromosome 3 of the plant.SELECTED DRAWING: None

Description

The present invention relates to Udonko disease, particularly Udonko disease caused by the phytopathogen Erysiphe heraclei, a resistant carrot plant or Daucus carota plant conferred Udonko disease resistance by one or two dominant Udonko disease resistance genes. The present invention further relates to molecular markers genetically associated with Udonco disease, particularly Udonco disease caused by the phytopathogen Erysiphe heraclei, resistance-imparting genes, and carrot or Daucus carota plants by Udonco disease, especially the phytopathogen Erysiphe heraclei. With respect to their use in determining resistance to the Udonco disease that occurs. The present invention also relates to the seeds, plant parts, and especially edible parts of the plant.

Carrots or Daucus carota are cultivated plants of the Umbelliferae family that are common in many parts of the world. Umbelliferae consists of a large number of species, many of which are aromatic plants with hollow stems, and are included in the 20 largest families of flowering plants. Along with the genus Daucus, other cultivated plants are known, such as caraway, celery, coriander, dill, fennel, parsley and parsnips. Umbelliferae includes more than 3,500 species in total.

The wild carrot Daucus carota L. is endemic in large parts of the world and has white taproots that are edible at a young age but become woody over long-term growth. The cultivated carrot, Daucus carota, especially Daucus carota spp. Sativus, is a root vegetable that is usually orange, but purple, red, yellow and white varieties are also known.

In mild climates, Daucus carota is usually a biennial plant, growing long in the first year after sowing and can bloom in the second year of cultivation after overwintering. In tropical and subtropical regions, carrots are annual and undergo a transition from growth to reproductive without vernalization. Some wild species have an annual life cycle.

The leaves are spirally attached. When the peduncle grows, the tip of this stem becomes sharp and becomes a finely branched inflorescence. This stem can be 60-200 cm long.

The flowers form umbels with white to light green or yellow pedicels. Individual flowers have a pedicel. The first umbel is attached to the tip of the main stem, and further umbels can grow from this main branch. Each flower has five petals, five stamens, and one stigma in the center. The flowers are male precocious, that is, the anthers release pollen before the stigma of the flower becomes pollinable. This mechanism prevents self-pollination to some extent and promotes cross-pollination. On the upper surface of the carpel, there is a pistil containing nectar. The flowers attract insects that mediate pollination, and when pollinated, the outer part of the umbel bends inward, and the umbel transforms into a cup shape via a convex shape. Seeds that grow after about 30 days consist of two fruit splits, each containing true seeds.

The appearance of male sterility in Daucus is a very useful property for the production of hybrid seeds. Two types of male sterility of the genus Daucus are known (references 1, 2, 3): the so-called brown anther type (anthers degenerate and deflate before pollen can be dissipated. ) And the petaloid type in which the stamens replace the petal-like structure (Reference 1).

The male sterility found in carrots is usually cytoplasmic male sterility, which means that the genetic determinants responsible for the trait are encoded by mitochondrial DNA rather than being present on the nuclear chromosome. .. The trait is maternally inherited because mitochondria are transmitted only by the egg cell to the offspring. The appearance of male sterility allows 100% cross-pollination, making Daucus hybrids easy to make. Carrots are inbreeding depression crops, but can be very strong if they are heterosis or hybrid.

Carrots are cultivated for their nutritious taproot. Most of the taproot consists of an outer phloem and an inner xylem. The phloem, which occupies most of the wood, is considered to be horticulturally valuable. Many taproots are known to have a round shape, a conical shape, or a more cylindrical shape, depending on the application. Root lengths vary from 5 cm to 40 cm and diameters can vary from 1 cm to 10 cm. The color of the taproot is white in wild species, but is often orange in cultivated species, and some are red, purple, black or yellow. Taproot is rich in carotene, an important antioxidant that can be metabolized to vitamin A, especially β-carotene. Carrots also contain dietary fiber, vitamins C, B6 and K, and the antioxidant falcarinol. Antioxidants (including carotenoids) have been studied for their ability to prevent chronic diseases. The free sugars are mainly sucrose, glucose and fructose.

Carrots are cultivated all over the world. In 2011, over 35 million tonnes of carrots were produced (Reference 4). Like all other human-grown crops, there are many factors that threaten good harvests in this crop. Diseases caused by many bacteria, fungi, viruses and viroids, as well as insect damage caused by many insects and nematodes are known. Diseases caused by major bacteria and fungi are caused by Xanthomonas campestris, Erwinia carotavora, Alternaria dauci, Alternaria radicina, Pythium spp., Rhizoctonia spp., Sclerotinia spp., Fusarium spp, Botrytis cinerea and Phytophthora spp. Nematodes such as Heterodera carotae, Meloidogyne spp. And Pratylenchus spp. Significantly damage taproots, resulting in reduced yields and unfit products. In addition, various viruses and viroids are known to adversely affect plant health and carrot yields.

Due to the reduced carrot yields caused by such pathogens, breeding programs have been promoted by companies and government agencies to introduce resistance to the pathogens. One of the diseases that greatly affects carrot cultivation is Udonko disease caused by Erysiphe heraclei. Erysiphe heraclei belongs to the ascomycete Erysiphales order and causes leaf disease powdery mildew in some Umbelliferae plant members (references 5 and 6). Several differentiated forms of E. heraclei are known and are generally specific to different genera of the Umbelliferae family.

When infected with Erysiphe heraclei, carrot plants become covered with fungi and spores. Fungi give rise to suckers that invade plant cells, but they do not cross the cell membrane. Therefore, fungi are present between cells. Through the sucker, nutrients and water are absorbed through the intercellular spaces of the infected plant. Fungal spots first appear on the lower leaves and spread to the upper plant parts. The spots spread throughout the plant, including the floral pattern, if any. This disease is most pronounced in summer-autumn temperatures. If the infection is severe, the leaves will fall, the yield will decrease, and the quality of the seeds will decrease in seeding. A 20% reduction in yield is not uncommon. In humid conditions, infected tissues are easily affected by other (secondary) pathogens, resulting in rapid leaf debilitation. Modern top lifters use leaves to pull carrots off the ground, so the affected crops lose their leaves and cannot be properly harvested.

One way to avoid the effects of Erysiphe heraclei infection may be the application of fungicides. However, the use of pesticides tends to be generally restricted, and public awareness is also towards avoiding the use of such chemicals. In addition, organic growers do not use fungicides in their cultivation. Therefore, it is clear that carrots resistant to Erysiphe heraclei are required in this field.

In a study by a Japanese research institute, an attempt was made to introduce resistance to Erysiphe heraclei by transgenic technology using the human lysozyme gene under the control of the constitutive CaMV 35S promoter. Several transformants showed improved Erysiphe heraclei resistance. This resistance was confirmed in offspring (Reference 7).

An object of the present invention is to partially, if not completely, solve the above problems in the art by using genetic material derived from Daucus genetic resource (germplasm). Specifically, a task of the present invention is, among other things, to provide carrots or Daucus carota plants that are resistant to Erysiphe heraclei or Udonko disease.

In particular, the above problems are solved by providing the plant, gene and molecular markers described in the claims.

Specifically, in particular, the above-mentioned problem is solved by the provision of a Daucus carota plant resistant to Udonko disease caused by the phytopathogen Erysiphe heraclei according to the first aspect of the present invention. It is conferred by a first resistance gene or Eh1 located between SEQ ID NO: 4 (also referred to as 9708 in the present invention) and SEQ ID NO: 5 (also referred to as 9625 in the present invention) on the third chromosome of the plant.

Alternatively, in particular, the above problem is solved by the provision of a Daucus carota plant resistant to Udonco disease caused by the phytopathogen Erysiphe heraclei according to the first aspect of the present invention, wherein the resistance is the first of the plants. On three chromosomes with either of the molecular markers identified as 9618, 9620, 9624, 9703 or 9708 on one side and any of the molecular markers identified as 9625, 9629, 9635, 9631 or 9636 on the other side. It is conferred by a first resistance gene located between or Eh1.

Alternatively, in particular, the above problem is solved by the provision of a Daucus carota plant resistant to Udonco disease caused by the phytopathogen Erysiphe heraclei according to the first aspect of the present invention, wherein the resistance is the first of the plants. By a first resistance gene or Eh1 located on three chromosomes between any of SEQ ID NOs: 1, 2, 3 or 4 on one side and any of SEQ ID NOs: 5, 6 or 7 on the other side Granted.

The genomic sequence of Daucus carota has been (partially) determined (Reference 8), and the sequence is generally available at NCBI under SEQ ID NO: PRJNA268187 (Reference 9). In the sequence of PRJNA268187, the first resistance gene or Eh1 of the present invention can be found between positions 1,648,619 and 1,739,519 on chromosome 3. Using the sequences presented herein, one of ordinary skill in the art will appreciate other commonly available sequence information of the Daucus carota genome, particularly between the positions of its third chromosome, eg, SEQ ID NO: 4 and SEQ ID NO: 5. The resistance gene of 1 or Eh1 can be easily identified. Alternatively, the first resistance gene or Eh1 of the present invention is found between any of SEQ ID NOs: 1, 2, 3 or 4 on one side and any of SEQ ID NOs: 5, 6 or 7 on the other side. be able to.

In a preferred embodiment of the first aspect of the invention, in the Daucus carota plant of the invention, resistance is further enhanced on the third chromosome of the plant with SEQ ID NO: 11 (also referred to as 9671 in the invention) and SEQ ID NO: 12 (also referred to as 9671 in the invention) It is also conferred by a second resistance gene or Eh2 located between (also referred to as 9672 in the present invention).

Alternatively, in said preferred embodiment of the invention, additional resistance is given to any of the molecular markers identified as 9695, 9666, 9669, 9670 or 9671 on one side of the plant on chromosome 3 and the other side. Is imparted by a second resistance gene or Eh2 located between any of the molecular markers identified as 9672, 6709, 9674, 9677, 9528, 6909, 4201 or 6069 in.

Alternatively, in said preferred embodiment of the invention, additional resistance is given to any of SEQ ID NOs: 8, 9, 10 or 11 on one side and SEQ ID NOs: 12, 13 on the other side on chromosome 3 of the plant. , 14 or 15 conferred by a second resistance gene or Eh2 located between.

The genomic sequence of Daucus carota has been (partially) determined and is generally available at NCBI under SEQ ID NO: PRJNA268187 (Reference 9). In the sequence of PRJNA268187, the second resistance gene of the invention or Eh2 can be found between positions 45,210,264 and 45,845,221 on chromosome 3. Using the sequences presented herein, one of ordinary skill in the art will appreciate other commonly available sequence information of the Daucus carota genome, particularly between the positions of its third chromosome, eg, SEQ ID NO: 11 and SEQ ID NO: 12. The resistance gene of 2 or Eh2 can be easily identified. Alternatively, the second resistance gene or Eh2 of the invention is between any of SEQ ID NOs: 8, 9, 10 or 11 on one side and any of SEQ ID NOs: 12, 13, 14 or 15 on the other side. Can be found in.

In another preferred embodiment, the first resistance gene or Eh1 of the invention is at position 2.68 cM on chromosome 3 and the second resistance gene or Eh2 of the invention is on chromosome 3. It exists at the position of 76.7 cM. As mentioned above, these chromosomal positions (centimorgans) correspond between positions 1,648,619 and 1,739,519 and between positions 45,210,264 and 45,845,221 on chromosome 3 in the sequence of PRJNA268187, respectively.

In yet another preferred embodiment, the first resistance gene or Eh1 of the present invention was prepared on March 19, 2015 at deposit number NCIMB 42389 (Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, United Kingdom). It can be obtained, obtained or derived from the seeds of the deposited Daucus carota plant. In other words, the first resistance gene or Eh1 of the present invention is preferably the resistance gene found in seed deposit NCIMB 42389.

In yet another preferred embodiment, the second resistance gene or Eh2 of the present invention was prepared on April 16, 2015 at deposit number NCIMB 42397 (Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, United Kingdom). It can be obtained, obtained or derived from the seeds of the deposited Daucus carota plant. In other words, the second resistance gene or Eh2 of the present invention is preferably the resistance gene found in seed deposit NCIMB 42397.

In a preferred embodiment of the invention, the first resistance gene or Eh1 of the invention is from SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7. It can be identified by at least one molecular marker selected from the group. In other words, the first resistance gene or Eh1 of the present invention is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7. Genetically linked to at least one molecular marker.

In a further preferred embodiment of the invention, the second resistance gene or Eh2 of the invention is SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 And at least one molecular marker selected from the group consisting of SEQ ID NO: 15. In other words, the second resistance gene or Eh2 of the present invention is a group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15. Genetically linked to at least one molecular marker selected from.

Considering the genetic linkage between the first resistance gene or Eh1 of the present invention and the molecular marker of the present invention, the present invention has, in a particularly preferred embodiment, in the genome, SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3. The Daucus carota plant comprising at least one genomic sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7.

Considering the genetic linkage between the second resistance gene of the present invention or Eh2 and the molecular marker of the present invention, the present invention has, in a particularly preferred embodiment, in the genome, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10. The Daucus carota plant comprising at least one genomic sequence selected from the group consisting of SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15.

In the most preferred embodiment, the invention is at least one selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7 in the genome. Daucus containing the genomic sequence and at least one genomic sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15. About carota plants.

The plant is preferably a hybrid plant, more preferably a sterile hybrid plant, most preferably a male sterile hybrid plant, such as cytoplasmic male sterile.

The Daucus carota plant of the present invention is preferably a Daucus carota ssp. Sativus plant.

The present invention relates, in other aspects, to the seed, edible portion, pollen, egg cell, callus, suspension culture, (indefinite) embryo, or plant portion of the Daucus carota plant.

Given the genetic linkage between the molecular markers of the invention and the resistance genes Eh1 and Eh2 of the invention, the invention is, in a further aspect, Daucus, which is resistant to Udonco disease caused by the phytopathogenic fungus Erysiphe heraclei. SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 10 for identifying the carota plant. 11. With respect to the use of one or more molecular markers selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15.

Furthermore, in view of the genetic linkage between the molecular markers of the invention and the resistance genes Eh1 and Eh2 of the invention, the invention in yet another aspect is resistant to Udonco disease caused by the phytopathogenic fungus Erysiphe heraclei. Is located at 2.6 cM on chromosome 3 and is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7. A gene identifiable by at least one molecular marker, as well as a gene located at 76.7 cM on chromosome 3, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 12. 13, relating to a gene identifiable by one or more molecular markers selected from the group consisting of SEQ ID NO: 14 and SEQ ID NO: 15.

The present invention will be further described with reference to the following examples and drawings.

FIG. 1 shows both the molecular markers of the invention and both the first resistance gene Eh1 of the invention and the second resistance gene Eh2 of the invention conferring resistance to the phytopathogenic fungus Erysiphe heraclei. The outline of the physical map of chromosome 3 is shown. FIG. 2 shows the sequences of the molecular markers SNP markers shown in FIG. 1 and their positions on chromosome 2 of the sequence of PRJNA268187 (Reference 9). SEQ ID NOs: 1 to 7 correspond to SNPs showing resistance genes at the Eh1 locus. SEQ ID NOs: 8 to 15 correspond to SNPs indicating resistance genes at the Eh2 locus. SEQ ID NOs: 16-22 correspond to SNPs indicating susceptibility genes at the Eh1 locus. SEQ ID NOs: 23-30 correspond to SNPs indicating susceptibility genes at the Eh2 locus. The use of the code follows the IUPAC nucleotide code below (Reference 10): A = adenine, T = thymine, C = cytosine, G = guanine, K = G or T, W = A or T, Y = C or T.

Testing resistance to Erysiphe heraclei in a greenhouse The fungus as an obligate parasite was maintained in suitable susceptible carrot plants by placing infected leaves between the plants. By using a fan, the airflow spread the spores between the plants and spread the infection between the plants.

The soil on the table was sown with about 30 plants per row to be tested for resistance. For every 20 rows of plants tested, each row of resistant and sensitive materials to race 0 and race 1 was sandwiched. When the plant reached a height of about 3 cm, it was inoculated by applying infected leaves that clearly showed fungal spores. First, the infected leaves were patted on the plant to be tested for resistance, and then the inoculum leaves were placed between the young plants. The spores were further diffused using a fan. The temperature was 16 ± 2 ° C. at night and 22 ± 2 ° C. during the day, with a minimum of 16 hours (or more if the photoperiod was longer) being bright and a maximum of 8 hours being dark. Humidity was maintained at a high level by spraying water between the tables several times a week. Plants were evaluated after 6 weeks; infected leaves were covered with white powdery mycelium and spores and were often bleached.

The degree of infection was expressed as a symptom rated on a score of 0 (fully sensitive) to 9 (fully resistant). Careful confirmation was made that susceptible control plants showed signs of E. heraclei infection.

Testing for resistance to E. heraclei outdoors An outdoor test was conducted in Dutch climatic conditions. Fungal inoculum was prepared as described in Example 1. The material to be tested outdoors was sown directly during the first half of May.

When the plant reached a height of about 3 cm, the inoculum was spread by placing a pot of sporulating plant on the ground between the young test plant materials. Spores are diffused by the wind from the inoculation plant.

The resistance or susceptibility of the plant was assessed when the signs were clearly visible during arid climatic conditions. The degree of infection was expressed as a symptom rated on a score of 0 (fully sensitive) to 9 (fully resistant).

Molecular characterization of genomic DNA and mapping of resistant genes Using the two available resistant genetic resources described above, each resistant resource was crossed with a susceptible carrot line and the resulting F1 plant was autologous. By pollination, two F1S1 populations were prepared. Since it was separated from the susceptible plant 1 with respect to the resistant plant 3, it was found that the resistance was based on the dominant trait in each case.

Basic research has revealed a partial genetic map of D. carota and an almost complete sequence of its genome, which has been submitted to NCBI as Project PRJNA268187 (Reference 9).

At least 2000 seeds were harvested from the F1S1 generation by mating a resource of apparent resistance with a susceptible carrot line. For QTL mapping, 1200 plants of each mating were grown in the greenhouse. Leaf material from each individual plant was used for DNA isolation and subsequent marker analysis.

Inbred strains of selected individuals with crossings near the resistance locus were tested in a greenhouse as described in Example 1 to confirm resistance.

To develop additional single nucleotide polymorphism (SNP) markers in the area of resistance genes, we have started a sequencing project using available resources of resistance to E. heraclei.

Using SNP markers covering the entire genome, it was determined that both resistance genes are located on chromosome 3. Using the sequences of two resistant strains and one susceptible strain in combination with the available genomic sequences, many SNPs were discovered for both resistance loci. Based on the crossovers present in the mapping population, the position of each resistance locus on the genomic sequence submitted to NCBI as PRJNA268187 (reference 9) could be determined.

For D. carota commissioned NCIMB 42389, the resistance locus (Eh1) was located at 2.68 cM on chromosome 3. This corresponds to the fragment between positions 1648169 and 1739519bp.

Using the sequences, additional SNP markers in the region of the resistance locus were developed and used for genotyping of resistant resources and individuals with crossings in the vicinity of the resistance locus. See Table 1 below.

^＊ マーカー９６３１について：物理的地図は当該領域において、交叉データに基づきマーカーの順序について修正された。

^{* For} markers 9631: The physical map has been modified for marker order in the area based on crossover data.

The resistance locus is located between marker 9618 and marker 9631.

Furthermore, for D. carota consignment NCIMB 42397, the second resistance locus (Eh2) was determined near 76.7 cM on chromosome 3. This corresponds to the fragment between positions 45210264bp and 45845221bp (Reference 9).

For genotype consignment NCIMB 42397, further markers could be developed based on information from the sequencing project, which was used for genotyping of genotype consignment NCIMB 42397 and crossover individuals. See Table 2 below.

This position can be shown graphically, as shown in FIG. The dominant resistance genes of the present invention are located at a distance on chromosome 3, as evidenced by both the position indicated by cM and the base pair position, and as shown in FIG. The discovery of two different resistance genes means that these resistance genes are preferably used in combination, for example as a hybrid, to provide a stronger genetic base for sustained resistance.

Deposit Information The seed sample of the resistant resource was deposited with NCIMB (Ferguson Building; Craibstone Estate, Bucksburn, Aberdeen, Scotland, AB21 9YA) as follows:
・ NCIMB 42389 (D. carota # 954561), March 19, 2015 ・ NCIMB 42397 (D. carota # 1360572), April 16, 2015

References

Aspects of the present invention will be further described:
[Item 1]
A Daucus carota plant resistant to powdery mildew caused by the phytopathogenic fungus Erysiphe heraclei, the first resistance of which resistance lies between SEQ ID NO: 4 and SEQ ID NO: 5 on chromosome 3 of the plant. Daucus carota plant conferred by sex genes.
[Item 2]
The Daucus carota plant according to Item 1 above, wherein the resistance is further imparted by a second resistance gene located between SEQ ID NO: 11 and SEQ ID NO: 12 on the third chromosome of the plant.
[Item 3]
The Daucus carota plant according to Item 1 or 2 above, wherein the first resistance gene is located at 2.68 cM on chromosome 3.
[Item 4]
The Daucus carota plant according to item 2 or 3 above, wherein the second resistance gene is located at 76.7 cM on chromosome 3.
[Item 5]
The Daucus carota plant according to any one of the above items 1 to 4, wherein the first resistance gene is obtained from the seeds of the Daucus carota plant deposited under the deposit number NCIMB 42389.
[Item 6]
The Daucus carota plant according to any one of Items 2 to 5 above, wherein the second resistance gene is obtained from the seeds of the Daucus carota plant deposited under deposit number NCIMB 42397.
[Item 7]
The first resistance gene is identified by at least one molecular marker selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7. The Daucus carota plant according to any one of the above items 1 to 6, which is possible.
[Item 8]
The second resistance gene is at least one selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15. The Daucus carota plant according to any one of Items 2 to 7 above, which can be identified by a molecular marker.
[Item 9]
Items 1 to 1 above, wherein the genome contains at least one genomic sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7. The Daucus carota plant according to any of 8.
[Item 10]
The genome comprises at least one genomic sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15. The Daucus carota plant according to any one of Items 2 to 9 above.
[Item 11]
The Daucus carota plant according to any one of Items 1 to 10 above, wherein the plant is a hybrid plant.
[Item 12]
The Daucus carota plant according to Item 11, wherein the hybrid plant is a sterile hybrid plant.
[Item 13]
Item 12. The Daucus carota plant according to Item 12, wherein the sterile hybrid plant is a male sterile, preferably cytoplasmic male sterile hybrid plant.
[Item 14]
The Daucus carota plant according to any one of Items 1 to 13 above, wherein the plant is Daucus carota spp. Sativus.
[Item 15]
The seed, edible portion, pollen, egg cell, callus, suspension culture, adventitious embryo, embryo, or plant portion of the Daucus carota plant according to any one of the above items 1 to 14.
[Item 16]
SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 6 for identifying Daucus carota plants resistant to Udonco disease caused by the phytopathogenic fungus Erysiphe heraclei. 7. Use of one or more molecular markers selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15 ..
[Item 17]
A gene that imparts resistance to Udonco disease caused by the phytopathogen Erysiphe heraclei, located at 2.6 cM on chromosome 3, SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 4. A gene that can be identified by at least one molecular marker selected from the group consisting of 5, SEQ ID NO: 6 and SEQ ID NO: 7.
[Item 18]
A gene that imparts resistance to Udonco disease caused by the phytopathogenic fungus Erysiphe heraclei, located at 76.7 cM on chromosome 3, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 11. A gene identifiable by one or more molecular markers selected from the group consisting of 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15.
[Item 19]
A Daucus carota plant comprising the resistance gene according to item 17, the resistance gene according to item 18, or the resistance gene according to item 17 and the resistance gene according to item 18.
The present invention will be further described with reference to the following examples and drawings.

Claims (19)

A Daucus carota plant resistant to powdery mildew caused by the phytopathogenic fungus Erysiphe heraclei, the first resistance of which resistance lies between SEQ ID NO: 4 and SEQ ID NO: 5 on chromosome 3 of the plant. Daucus carota plant conferred by sex genes. The Daucus carota plant according to claim 1, wherein the resistance is further conferred by a second resistance gene located between SEQ ID NO: 11 and SEQ ID NO: 12 on the third chromosome of the plant. The Daucus carota plant according to claim 1 or 2, wherein the first resistance gene is located at 2.68 cM on chromosome 3. The Daucus carota plant according to claim 2 or 3, wherein the second resistance gene is located at 76.7 cM on chromosome 3. The Daucus carota plant according to any one of claims 1 to 4, wherein the first resistance gene is obtained from the seeds of the Daucus carota plant deposited under deposit number NCIMB 42389. The Daucus carota plant according to any one of claims 2 to 5, wherein the second resistance gene is obtained from the seeds of the Daucus carota plant deposited under deposit number NCIMB 42397. The first resistance gene is identified by at least one molecular marker selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7. The Daucus carota plant according to any one of claims 1 to 6, which is possible. The second resistance gene is at least one selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15. The Daucus carota plant according to any of claims 2 to 7, which can be identified by a molecular marker. Claims 1 to 1, wherein the genome comprises at least one genomic sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7. The Daucus carota plant according to any of 8. The genome comprises at least one genomic sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15. The Daucus carota plant according to any one of claims 2-9. The Daucus carota plant according to any one of claims 1 to 10, wherein the plant is a hybrid plant. The Daucus carota plant according to claim 11, wherein the hybrid plant is a sterile hybrid plant. The Daucus carota plant according to claim 12, wherein the sterile hybrid plant is a male sterile, preferably cytoplasmic male sterile hybrid plant. The Daucus carota plant according to any one of claims 1 to 13, wherein the plant is Daucus carota spp. Sativus. The seed, edible portion, pollen, egg cell, callus, suspension culture, adventitious embryo, or plant portion of the Daucus carota plant according to any one of claims 1-14. SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 6 for identifying Daucus carota plants resistant to Udonco disease caused by the phytopathogenic fungus Erysiphe heraclei. 7. Use of one or more molecular markers selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15 .. A gene that imparts resistance to Udonco disease caused by the phytopathogen Erysiphe heraclei, located at 2.6 cM on chromosome 3, SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 4. A gene that can be identified by at least one molecular marker selected from the group consisting of 5, SEQ ID NO: 6 and SEQ ID NO: 7. A gene that imparts resistance to Udonco disease caused by the phytopathogenic fungus Erysiphe heraclei, located at 76.7 cM on chromosome 3, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 11. A gene identifiable by one or more molecular markers selected from the group consisting of 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15. A Daucus carota plant comprising the resistance gene according to claim 17, the resistance gene according to claim 18, or the resistance gene according to claim 17 and the resistance gene according to claim 17.

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