JP2020159863A - Chromatographic medium and manufacturing method of the same - Google Patents

Abstract

To provide a chromatographic medium capable of easily determining whether detected substance is present in a specimen by clear colouring in a detector.SOLUTION: A detector at least contains fiber, and 70% or more of fiber contained in the detector is oriented in a direction perpendicular to a direction of travel from a specimen supply part to a specimen absorption part. Thus, an undried detection body is less likely to spread toward the specimen supply part and the specimen absorption part, is fixed in the orientation direction of the fiber, resulting in the detection body of the detector which is locally dense linearly. Accordingly, detected substances captured by the detector are similarly densely accumulated when the chromatographic medium is used. In addition, not only in formation of the detector, but also in use of the chromatographic medium, the detection body can be restrained from flowing toward the specimen absorption part due to capillary phenomenon of specimen itself. Accordingly, linear clear colouring occurs in the detector, which facilitates determination of whether the detected substance is present.SELECTED DRAWING: Figure 1

Description

The present invention relates to a chromatographic medium for detecting a substance to be detected (antigen, etc.) in a sample, and a method for producing the same.

A chromatographic medium used for detecting an antigen or the like generally has a short side and a long side provided with a base material that supports various materials and a chromatography unit provided on the upper surface of the base material. It has an elongated shape with. The chromatography section has a sample supply section on one end side in the long side direction, a sample absorption section on the other end side, and a direction orthogonal to the long side direction (in the short side direction) in the middle portion in the long side direction. It has a configuration having a detection unit that appears in a line shape (in the parallel direction).

An immunochromatography method using an antigen-antibody reaction is often used to detect a substance to be detected in a sample. In the immunochromatography method, the substance to be detected is generally labeled with some kind of labeling substance for the purpose of simplifying the determination and improving the accuracy.

Specifically, a labeling substance for assisting the determination is added to a substance that specifically binds to the substance to be detected to form a labeling substance, and the substance to be detected is labeled by binding to the labeling substance. To become. An antigen-antibody reaction is often used to bind the substance to be detected and the labeling substance.
There are various methods for detecting a substance to be detected using an antigen-antibody reaction. As an example, colloidal gold immunization is often used as a rapid diagnostic agent. This method uses a labeling substance using gold colloid as a labeling substance.

Specifically, first, the substance to be detected is labeled with a gold colloid-labeled antibody (labeling substance). Next, the labeled substance to be detected is captured and accumulated by the detection unit. Then, the determination is made by confirming the color tone of the gold colloid accumulated in the detection unit. When a detector that specifically binds to the substance to be detected is used as the detector, if the substance to be detected is not present in the sample, the labeling substance alone will not be captured by the detection unit, so that the detection unit will not develop color. Absent.

The chromatograph medium contains a sample diluent for dissolving or diluting the substance to be detected, an additive that suppresses side reactions and non-specific reactions, an antibacterial agent, a protective stabilizer for the sample solution, a dissolution accelerator, and a label. Some contain substances in advance. In addition, some of these are mixed with a sample solution and used as a chromatographic medium.

In the chromatograph medium, a liquid sample is dropped onto the sample supply section, and when the sample advances from the sample supply section to the sample absorption section by capillarity, the substance to be detected (for example, human chorionic gonadotropin) is added to the sample. (HCG))) is present, the detector captures the substance to be detected at the detection unit and causes the detection substance to develop a color. (The following Patent Documents 1 and 2 exist as prior documents similar to this).

Japanese Unexamined Patent Publication No. 2013-53869 Japanese Unexamined Patent Publication No. 2017-15532

The chromatograph media of Patent Document 1 and Patent Document 2 are designed so that when a substance to be detected (for example, hCG) is present in the sample, the detection unit develops a line-like color, but the color development is unclear. Therefore, it may be difficult to determine whether or not the detected substance is present.
The present inventor has diligently studied the reason why the color development of the detection unit becomes unclear, and found the following reason.

The detection unit is formed by applying the detector to the chromatography unit and drying it. When the detector is formed, the detector before drying spreads toward the sample supply unit and the sample absorption unit, so that the detector diffuses, and the diffusion of the detector reduces the concentration of the detector in the detector. .. When this is used as a chromatograph medium, the concentration of the substance to be detected captured by the detection unit also decreases, so that the color of the detection unit becomes light and unclear when the chromatograph medium is used.

An object of the present invention is to provide a chromatographic medium capable of solving the above problems, clarifying color development in a detection unit, and easily determining whether or not a substance to be detected is present in a sample.

The present invention has, for example, the following configuration as a means for solving the above-mentioned problems and achieving the above-mentioned object.
That is, in a chromatographic medium having a sample supply unit and a detection unit, the sample supplied to the sample supply unit advances and moves from the sample supply unit toward the detection unit, and the detection unit contains fibers. It is characterized in that 70% or more of the contained fibers are oriented in a direction orthogonal to the traveling direction.

The fiber is a chemical fiber.
The method for producing a chromatographic medium, which comprises a step of printing a dispersion containing fibers on a substrate.

A method for producing a chromatograph medium, which comprises a drying step of drying the dispersion printed in the printing step, a coating step of applying a detection body in the same direction as the printing direction of the printing step, and the detection body. A chromatography molding step of molding a chromatography section having a detection section formed by drying, and a chromatograph medium obtained by cutting the molded chromatography section in a direction orthogonal to the printing direction of the printing step. A method for producing a chromatograph medium, which comprises a cutting step for obtaining the above.

According to the present invention, it is possible to provide a chromatographic medium that can clarify the color development in the detection unit and can easily determine whether or not the substance to be detected is present in the sample.

It is a perspective view of the chromatographic medium which concerns on one Embodiment which concerns on this invention. It is a flowchart for demonstrating the manufacturing method of the chromatograph medium in this embodiment. It is a figure for demonstrating the detail of the cutting process in this embodiment.

It is a figure which shows the color development result of the detection part 4 of the chromatographic medium of Example 1. FIG. It is a figure which shows the color development result of the detection part 4 of the chromatographic medium of the comparative example 1. FIG. It is a figure which shows the color development result of the detection part 4 of the chromatographic medium of Example 2.

It is a figure which shows the color development result of the detection part 4 of the chromatographic medium of the comparative example 2. It is a figure which shows the color development result of the detection part 4 of the chromatographic medium of the comparative example 3. FIG.

Hereinafter, embodiments according to the present invention will be described in detail with reference to the drawings.
FIG. 1 is a perspective view of a chromatographic medium according to an embodiment of the present invention. In FIG. 1, 1 is a base material, 2 is a chromatography section, 3 is a sample supply section, 4 is a detection section, and 5 is a sample absorption section.
The chromatography unit 2 can move the sample by a capillary phenomenon, and by forming the detection unit 4 on which the detector is fixed, the substance to be detected can be captured. The direction of travel from the sample supply unit 3 to the sample absorption unit 5 here refers to the direction of 7 in FIG. That is, it refers to the direction from the sample supply unit 3 in FIG. 1 to the sample absorption unit 5 in FIG. 1 when the chromatograph medium is processed into individual chromatographic media.

The chromatographic medium in the present embodiment suppresses the detection substance from spreading toward the sample supply unit 3 and the sample absorption unit 5 when the detection unit 4 is formed, whereby the concentration of the detection substance in the detection unit 4 is reduced. By preventing the above, the color development in the detection unit 4 is clarified, and it is possible to easily determine whether or not the substance to be detected is present in the sample.

Therefore, at least the detection unit 4 contains fibers, and 70% or more of the fibers contained in the detection unit 4 are oriented in the direction 8 orthogonal to the traveling direction 7 from the sample supply unit 3 to the sample absorption unit 5. It is characterized by having an orthogonal configuration. Further, the fibers of the detection unit 4 can be composed of chemical fibers. The fiber of the embodiment of the present embodiment refers to a material having a fine thread-like shape.

Further, the method for producing a chromatographic medium according to the present embodiment will be described with reference to FIG. FIG. 2 is a flowchart for explaining a method for manufacturing a chromatographic medium according to the present embodiment.

First, in step S1, as a first step, a dispersion base material printing step of printing a dispersion containing fibers on a (large format) base material 1 having a predetermined size is performed. At this time, by printing the dispersion containing the fibers in the printing direction 6 of FIG. 3, the fibers are oriented in the printing direction 6.

Subsequently, as a second step in step S2, a dispersion drying step of drying the dispersion printed on the base material 1 is executed. After that, in step S3, as a third step, the detection body coating step of coating the detection body in the same direction as the printing direction of the dispersion is executed. Here, for example, a liquid sample (detector) containing an anti-hCG monoclonal antibody or the like is printed and applied to the detection unit of the chromatography unit in the printing direction 6 and immobilized on the chromatography unit.

Further, in step S4, as a fourth step, a detector drying step of drying the detector applied in step S3 is executed, and a chromatography section having the formed detector is formed.

In the next step S5, as a fifth step, the chromatographic medium having the chromatographic part molded in the steps up to step S4 is cut in a direction orthogonal to the printing direction in the step S1 to chromatographic pieces. Obtain a graph medium.

In the present embodiment, the large-format chromatography section 2 is formed by printing the dispersion containing fibers on the large-format substrate 1 in the printing direction 6. The details of the cutting step in step S5 will be described below with reference to FIG. FIG. 3 is a diagram for explaining the details of the cutting process in the present embodiment.

In the cutting step, as shown in FIG. 3, first, the absorption pads 9 are attached to a part of the upper surface of the sample absorption portion by using double-sided tape or the like.
After that, it is cut by a cutter or the like along the lines aa', bb', cc'and dd'shown in FIG. The individual test strips shown in FIG. 1 are prepared by sequentially cutting with a cutter along the widths of the aa'and bb' lines.

The chromatographic medium of the present embodiment can be obtained, for example, by printing a dispersion containing fibers on a substrate. Even if the dispersion containing the fibers is printed on a substrate having the size of an individual chromatograph medium, the dispersion containing the fibers is printed on a large-sized substrate, and then the individual chromatograph medium is printed. You may cut it to size.
When printing a dispersion containing fibers on a substrate, for example, by printing in a direction orthogonal to the traveling direction from the sample supply section to the sample absorption section when using a chromatograph medium, the fibers are oriented in the printing direction. be able to.

Note that printing in the present embodiment refers to a method of applying a dispersion containing fibers to an object (base material or the like), and is used as a method of forming a chromatography section in the present embodiment. ing.

As a printing method, various printing methods can be used, and examples thereof include letterpress printing technology, flexographic printing technology, screen printing technology, spray coating, and other casting techniques.

The fiber-containing dispersion can be produced by dispersing the chemical fiber in water or an organic solvent. In order to have a viscosity suitable for printing and to improve the strength of the obtained sheet, a liquid-philic polymer may be appropriately mixed. Examples of the lipophilic polymer include oxyethylene compounds such as polyoxyethylene and polyoxypropylene, amide compounds such as polyacrylic amide, vinyl alcohol compounds such as polyvinyl alcohol, acrylic compounds, ester compounds, and starch. Examples include nanofibers obtained by mechanically treating a hydrophilic-treated olefin compound, bacterial cellulose, pulp and the like, and cellulose nanofibers such as nanofibers obtained by chemically treating pulp and the like. .. When cellulose nanofibers are obtained by processing pulp or the like, chemical pulp such as kraft pulp or sulfite pulp or mechanical pulp such as grind pulp or thermomechanical pulp can be used as the material pulp. Can use wood pulp and non-wood pulp.

The chromatographic medium of the present invention may form a chromatography section by a method other than printing. For example, by using a raw material containing fibers and forming a sheet with a circular net paper machine or the like, a sheet in which fibers are oriented can be produced. The method of applying and printing the detector is not particularly limited as long as a dispenser, an inkjet or the like may be used.

In the description of the above embodiment, the detection unit 4 is formed by linearly applying and printing the detection body in the printing direction 6, but the detection unit 4 is formed by arranging patterns such as small circles and triangles. Is also good.
Further, in the examples of FIGS. 1 and 3, the detection unit 4 is provided at two locations, but it may be provided at one location or at three or more locations if necessary. Further, when a plurality of detection units 4 are provided, one of them may be used as a control unit for displaying the end of the test.

In the chromatograph medium of the present embodiment, at least the detection unit contains fibers, and 70% or more of the fibers contained in the detection unit are in a direction orthogonal to the traveling direction from the sample supply unit to the sample absorption unit. Because of the orientation, the detector before drying is less likely to spread toward the sample supply section and the sample absorption section, is fixed along the fiber orientation direction, and the detector in the detection section is densely lined. Orthogonal. Therefore, when the chromatograph medium is used, the substance to be detected captured by the detection unit is also densely accumulated.

Furthermore, not only when the detection unit is formed, but also when the chromatograph medium is used, it is possible to prevent the detector from being swept away toward the sample absorption unit due to the capillary phenomenon of the sample itself, so that the detection unit develops a clear line and is detected. It becomes possible to easily determine whether or not a substance exists.

The chromatography section is a flow path for moving the sample from the sample supply section to the sample absorption section by using the capillary phenomenon, and when the sample contains a substance to be detected, the detection is performed. It has a detection unit that confirms a substance by various detection means. In the detection unit, the substance to be detected is captured and accumulated by the detector that binds to the substance to be detected in the sample.

In this embodiment, an immunochromatographic method using an antigen-antibody reaction is used to detect the substance to be detected. In the sample supply section, the sample supplied to the chromatographic medium to start the test, and in the sample absorption section, the sample supplied from the sample supply section to the chromatography section passed through the detection section. After that, it refers to the part for absorbing excess sample.

When a labeling substance is used, an enzyme, a coloring substance, a fluorescent substance, a radioactive substance, or the like can be arbitrarily used as the labeling substance. In selecting the labeling substance, the type of the substance to be detected, the labeling substance, and the type of the detector may be taken into consideration so as to take advantage of the characteristics of the immunochromatography method that the operation is easy and the determination time is short.

In addition, the immunochromatography method generally has a feature that it can be accurately determined by visual determination, but when time, accuracy, etc. are required, various detection means such as spectrophotometric detection and radiation detection. May be detected by.
Sample pads, conjugate pads, and absorbent pads may be used. When these are used, conventional ones can be used without particular limitation.

The chromatography unit can move the sample by a capillary phenomenon, and by forming a detection unit in which the detector is fixed, it is possible to capture the substance to be detected.
The direction of travel from the sample supply section to the sample absorption section referred to here refers to the direction of 7 in FIG. That is, it refers to the direction from the sample supply unit 3 in FIG. 1 to the sample absorption unit 5 in FIG. 1 when the chromatograph medium is processed into individual chromatographic media.

Further, the direction orthogonal to the traveling direction 7 from the sample supply unit 3 to the sample absorption unit 5 refers to the direction 8 in FIG. That is, when the individual chromatographic medium is viewed from above, it refers to a direction orthogonal to the traveling direction from the sample supply unit 3 to the sample absorption unit 5. Further, in the embodiment of the present embodiment, when the fiber is tilted at an angle of −45 ° to + 45 ° from the direction orthogonal to the traveling direction from the sample supply unit 3 to the sample absorption unit 5, the sample supply unit 3 It is defined that the orientation is orthogonal to the traveling direction to the sample absorbing unit 5.

When confirming the orientation angle of the fibers, the method / apparatus capable of measuring the orientation angle is not particularly limited. For example, the chromatography section is magnified using a scanning electron microscope, and 100 fibers randomly selected. It can be obtained by measuring the angle of.
That is, when the fibers oriented in the direction 8 orthogonal to the traveling direction from the sample supply unit 3 to the sample absorption unit 5 are less than 70% of the fibers contained in the detection unit 4, when the detection unit 4 is formed, before drying. The detector is likely to diffuse to the sample supply unit 3 and the sample absorption unit side 5, and when the chromatograph medium is used, the detector is likely to be swept away to the sample absorption unit side by the capillary phenomenon of the sample itself. The color development of the detection unit 4 may be unclear.

When 70% or more of the fibers contained in the detection unit 4 are oriented in the direction 8 orthogonal to the traveling direction 7 from the sample supply unit 3 to the sample absorption unit 5, when the detection unit 4 is formed, before drying. The detector is deeply localized in the direction orthogonal to the traveling direction from the sample supply unit 3 to the sample absorption unit 5. Therefore, when the chromatograph medium is used, the detection unit 4 can capture and accumulate the substance to be detected in a narrow range, and the concentration of the substance to be detected in the detection unit 4 can be increased.

Further, when the chromatograph medium is used, it is possible to prevent the detector from being swept away to the sample absorbing portion due to the capillary phenomenon of the sample itself. As a result, the color development of the detection unit 4 when the substance to be detected is actually detected becomes clear, and it becomes possible to easily determine whether or not the substance to be detected exists.

If 70% or more of the fibers contained in the detection unit 4 are oriented in a direction orthogonal to the traveling direction from the sample supply unit 3 to the sample absorption unit 5, a location other than the detection unit 4 of the chromatography unit 2 There is no particular limitation on the orientation direction of.
The chromatographic medium of the present embodiment preferably contains chemical fibers as fibers. The chemical fiber of the present embodiment refers to an artificially produced fiber, and synthetic fiber, semi-synthetic fiber, regenerated fiber, inorganic fiber and the like can be used.

By containing the chemical fiber, 70% or more of the fiber contained in the detection unit 4 can be easily oriented in a direction orthogonal to the traveling direction from the sample supply unit 3 to the sample absorption unit 5. When it does not contain chemical fibers, for example, when it is composed only of natural cellulose fibers, the natural cellulose fibers are more flexible than the chemical fibers, so that the fibers are likely to be broken, bent, entangled, etc. when forming the chromatography section 2, and the orientation It can be difficult to control.

The fiber diameter and fiber length of the chemical fiber of the embodiment of the present invention are not particularly limited, and for example, those having a fiber diameter of about 0.1 μm to 50 μm and a fiber length of about 0.1 to 10 mm can be used. it can.
The material of the chemical fiber is not particularly limited, and examples of the synthetic fiber include nylon, aramid, vinylon, polyvinylidene chloride, polyvinyl chloride, polyester, polyacrylonitrile, polyethylene, polypropylene, polyurethane, polyclar, and polylactic acid. Examples of the recycled fiber include rayon, cupra, polynosic and the like, semi-synthetic fiber includes acetate and promix, and inorganic fiber includes glass fiber and carbon fiber. The above materials may be used alone or mixed appropriately.

For the detection unit 4 formed in the chromatography unit 2, a detector that specifically binds to the substance to be detected may be selected, and is not particularly limited. When an antigen-antibody reaction is used for detection, the detector may be an antigen or an antibody as long as it specifically binds to the substance to be detected.

When the substance to be detected is an antigen, a monoclonal antibody, a polyclonal antibody or a fragment thereof (first reagent) can be used for the detection unit 4 formed in the chromatography unit 2, and these can be used as a detector. Monoclonal antibodies, polyclonal antibodies or fragments thereof may be generally available or adjustable, and may be used without particular limitation. Further, the antibody-producing animal species can be selected without particular limitation, such as human, mouse, rat, rabbit, and goat. The immunoglobulin may be any of IgG, IgM, IgA, IgE and IgD.

When the substance to be detected is an antibody, an artificially prepared antigen that specifically binds to the antibody can also be used for the detection unit.
In the chromatograph medium of the embodiment of the present invention, the test sample (sample) to be analyzed includes, for example, blood, serum, plasma, urine, saliva, sweat, spinal fluid, tears, sheep water, papillary secretion, and the like. Not only biological samples such as nasal juice, sputum, nasal aspirate, pharyngeal swab, alveolar lavage fluid, exudate from skin, rectal wipe, feces and extracts from tissues and cells, but also food extracts, clean water, Samples such as sewage, culture solution, etc. can be mentioned, and are not particularly limited.

The substance to be detected in the chromatographic medium is not particularly limited as long as a substance that specifically binds to the substance to be detected exists or can be produced. When an antigen-antibody reaction is used for detection, the substance to be detected may be an antigen or an antibody as long as it specifically binds to the detector. In the case of an antigen, it is a chemical variant even if the substance to be detected is a complete antigen that is itself antigenic, or a hapten (incomplete antigen) that is not itself antigenic. Therefore, it may have antigenicity. In the case of an antibody, it may be either a monoclonal antibody or a polyclonal antibody.

To give an example of the substance to be detected according to the embodiment of the present invention, peptide hormone (growth hormone (GH)), adrenal cortex stimulating hormone (ACTH), melamine cell stimulating hormone (MSH), prolactin, thyroid stimulating hormone (TSH), Leprosy formation hormone (LH), follicular stimulating hormone (FSH), pituitary hormone, calcium metabolism regulatory hormone, pancreatic hormone, gastrointestinal hormone, vasoactive hormone, human chorionic gonadotropin (hCG), follicular hormone such as estron, progest Natural or synthetic luteinizing hormones such as Ron, male hormones such as testosterone, corticosteroids such as cortisol, hormones such as thyroid hormones such as diodesylonine, prostatic acidic phosphatase (PAP), prostate-specific antigen (PSA), Alkaline phosphatase, transaminase, trypsin, pepsinogen, α-fetoprotein (AFP), cancer-specific substances such as cancer fetal antigen (CEA), serum protein components such as immunoglobulin G (IgG), rheumatoid factor, serotonin, urokinase, ferritin, Other steroids such as substance P, fecal occult blood, syphilis antibody, influenza virus, adenovirus, rotavirus, mycoplasma, HBs antigen, HBs antibody, chlamydia antigen, group A β-lytic bacterium antigen, cholesterol, bile acid, cardiotonic steroid, sapogenin, etc. , Epinephrine, dopamine, physiologically active alkaloids, amino group-containing hormonal drugs, low molecular weight peptides such as TRH, prostaglandins, vitamins, antibiotics such as penicillin, other in vivo components, in vivo administered drugs And its metabolites and the like.

Hereinafter, various specific examples and comparative examples according to the present invention will be described in detail. When the chromatographic medium of the present embodiment is obtained by printing, it can be obtained, for example, as follows.
[Example 1]
1. 1. Preparation of Chromatography Part 15.3 parts of polyethylene terephthalate (PET) fiber having a fiber diameter of 12.5 μm and a fiber length of 0.2 mm, and aqueous dispersion of cellulose nanofibers obtained by mechanically treating broad-leaved bleached kraft pulp (LBKP). A dispersion containing fibers was prepared by mixing 45.3 parts of the body and 39.4 parts of a 0.5% polyoxyethylene aqueous solution as a total of 100 parts. Using a coating machine (PI-1210, manufactured by Tester Sangyo), the dispersion was applied onto the surface of commercially available water resistant paper and dried in an oven to prepare a chromatography section 2.
95% of the fibers of the chromatography section 2 were oriented in the direction orthogonal to the traveling direction from the sample supply section to the sample absorption section (orientation in the printing direction 6).

2. 2. Preparation of chromatographic medium having a chromatographic part with a detection part Using a commercially available micropipette, anti-anti-concentration of 2.0 mg / ml diluted with phosphate buffer (pH 7.2) containing 20% by mass of sucrose. 0.3 μL of hCG monoclonal antibody (Anti-h, Alpha buffer, chroma code / 6601, manufactured by Medix Biochemica) was applied in the printing direction 6 to form a detection unit 4. Subsequently, an anti-mouse IgG1 antibody (Goat anti-Mouse IgG1 antibody, manufactured by BETHYL) diluted with a phosphate buffer solution (pH 7.2) at a concentration of 1.0 mg / ml was applied in the same manner, and the second detection unit 4 was applied. Formed. Then, it was dried to prepare a chromatographic medium having a chromatographic unit provided with the detection unit 4. The second detection unit 4 serves as a control unit for confirming the end of the test.

3. 3. Preparation of Individual Chromatograph Medium A commercially available absorption pad (CF5, manufactured by Whatman Co., Ltd.) was attached to a part of the upper surface of the sample absorption unit 6 with double-sided tape.
Then, it was cut with a cutting machine in a direction orthogonal to the printing direction 6 so as to have a width of 4 mm, and further cut so as to have a length of 60 mm at the top and bottom to prepare a chromatographic medium of individual pieces.

4. Color development test using immunoassay Development solution (50 mM Tris, 0.14 M NaCl, 0.05% Tween 20, pH 8.0) and blocking agent (50 mM Tris, 0.14 M NaCl, 1% BSA, pH 8.0) ) Was mixed at a ratio of 1: 1 to prepare 105 μL, and commercially available human hCG (Sigma Aldrich) was added to 100 mIU / mL, and a commercially available colloidal gold-labeled hCG antibody (Anti-h, beta antibody) was added. 5 μL of 40 nm colloidal gold (manufactured by abcam) was added. The sample supply unit 3 of the chromatographic medium described above was immersed in the test solution, and the test solution was sucked up to develop a color in the detection unit. The color development result of the detection unit 4 of the chromatographic medium of Example 1 is shown in FIG.

[Comparative Example 1]
When the antibody reagent was applied to the detection unit 4, it was applied in a direction orthogonal to the printing direction 6. Further, when the individual chromatographic medium is formed, it is cut in a direction parallel to the printing direction 6, and the fibers of the chromatography section 2 are parallel to the traveling direction from the sample supply section to the sample absorption section. The test was carried out with the same contents as in Example 1 except that the state was changed to the oriented state. The results are shown in FIG.
In addition, 20% of the fibers of the chromatography section 2 were oriented in a direction orthogonal to the traveling direction from the sample supply section to the sample absorption section (orientation in the printing direction 6).

[Example 2]
In the preparation of the chromatography section, 11.7 parts of nylon fiber having a fiber diameter of 10.5 μm and a fiber length of 0.2 mm and 34.7 parts of an aqueous dispersion of cellulose nanofibers obtained by mechanically treating LBKP were 0 parts. A dispersion containing fibers was prepared by mixing 53.6 parts of a 5.5% polyoxyethylene aqueous solution as a total of 100 parts. Subsequent steps were carried out in the same manner as in Example 1. The results are shown in FIG.
In addition, 72% of the fibers of the chromatography section 2 were oriented in a direction orthogonal to the traveling direction from the sample supply section to the sample absorption section (orientation in the printing direction 6).

[Comparative Example 2]
A chromatographic medium was prepared and tested in the same manner as in Example 2 except that the detection portion was formed and cut in the same direction as in Comparative Example 1. The results are shown in FIG.
In addition, 6% of the fibers of the chromatography section 2 were oriented in a direction orthogonal to the traveling direction from the sample supply section to the sample absorption section (orientation in the printing direction 6).

[Comparative Example 3]
In the preparation of the chromatography part, 4.5 parts of LBKP having a fiber length of about 0.8 mm, 14.3 parts of an aqueous dispersion of cellulose nanofibers obtained by mechanically treating LBKP, 0.5% polyoxyethylene A liquid in which fibers were dispersed was prepared by mixing 81.2 parts of the aqueous solution as a total of 100 parts. Subsequent steps were carried out in the same manner as in Example 1. The results are shown in FIG.
In addition, 60% of the fibers of the chromatography section 2 were oriented in a direction orthogonal to the traveling direction from the sample supply section to the sample absorption section (orientation in the printing direction 6).

In the chromatographic medium of the example, 72 to 95% of the fibers are oriented in a direction orthogonal to the traveling direction from the sample supply section to the sample absorption section.
As is clear from FIGS. 4 to 7, the chromatographic media of Examples 1 and 2 had a clearer color development in the detection section than the chromatographic media of Comparative Examples 1 and 2.

In Comparative Example 3, individual pieces of chromatograph medium were produced by printing and cutting in the same direction as in Examples 1 and 2, but the fibers were oriented in a direction orthogonal to the traveling direction from the sample supply section to the sample absorption section. The ratio was as low as 60%, and as is clear from FIG. 8, the color development of the detection part was unclear. In Comparative Example 3, since the chromatographic part was formed only with natural cellulose fibers, it is considered that the cause was that the orientation of the fibers was disturbed. From this, it can be seen that it is preferable that the chromatography section contains chemical fibers.

In the chromatographic medium of the example, since the detection bodies are densely accumulated in a line when the detection unit is formed, the concentration of the complex of the detection body of the detection unit and the substance to be detected is also high when the chromatograph medium is used. Conceivable. From this, it can be seen that the color development of the chromatographic medium can be clarified by orienting 70% or more of the fibers of the detection unit in the direction orthogonal to the traveling direction from the sample supply unit to the sample absorption unit.

As described above, according to the present embodiment, in the chromatograph medium, at least the detection unit contains fibers, and 70% or more of the fibers contained in the detection unit is transferred from the sample supply unit to the sample absorption unit. Since the detector is oriented in a direction orthogonal to the traveling direction of the fiber, the detector before drying is less likely to spread toward the sample supply part and the sample absorption part, and is fixed along the fiber orientation direction, and the detection part is fixed. Detectives are deeply localized in a line. Therefore, when the chromatograph medium is used, the substance to be detected captured by the detection unit is also densely accumulated.

Further, not only when the detection unit 4 is formed, but also when the chromatograph medium is used, it is possible to prevent the detector from being swept away to the sample absorption unit side due to the capillary phenomenon of the sample itself. It becomes possible to easily determine whether or not the detected substance is present.

The present invention can be applied to a hormone test represented by a test for human chorionic gonadotropin (hCG), and a test using an immunological antigen-antibody reaction such as a cancer marker, an infectious disease, or a blood protein test.

1 Base material 2 Chromatography unit 3 Specimen supply unit 4 Detection unit 5 Specimen absorption unit 6 Printing direction 7 Direction of travel from sample supply unit to sample absorption unit 8 Direction orthogonal to the direction of travel from sample supply unit to sample absorption unit 9 Absorbent pad

Claims (4)

A chromatographic medium having a sample supply unit and a detection unit.
The sample supplied to the sample supply unit advances and moves from the sample supply unit toward the detection unit, the detection unit contains fibers, and 70% or more of the contained fibers are orthogonal to the traveling direction. A chromatographic medium characterized by being oriented in a direction. The chromatographic medium according to claim 1, wherein the fiber is a chemical fiber. The method for producing a chromatographic medium according to claim 1 or 2.
A method for producing a chromatographic medium, which comprises a step of printing a dispersion containing fibers on a substrate. The method for producing a chromatographic medium according to claim 3.
A drying step of drying the dispersion printed in the printing step, and a drying step of drying the dispersion.
A coating step of applying the detector in the same direction as the printing direction of the printing process, and
A chromatography molding step of molding a chromatography part having a detection part formed by drying the detector, and
A cutting step of cutting the molded chromatography section in a direction orthogonal to the printing direction of the printing step to obtain a chromatographic medium of individual pieces.
A method for producing a chromatographic medium having.