JP2020159695A - Wiping inspection kit - Google Patents

Wiping inspection kit Download PDF

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JP2020159695A
JP2020159695A JP2019055920A JP2019055920A JP2020159695A JP 2020159695 A JP2020159695 A JP 2020159695A JP 2019055920 A JP2019055920 A JP 2019055920A JP 2019055920 A JP2019055920 A JP 2019055920A JP 2020159695 A JP2020159695 A JP 2020159695A
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hollow
container body
hollow portion
cotton swab
wiping
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JP7231211B2 (en
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和雄 白岩
Kazuo Shiraiwa
和雄 白岩
宗洙 李
Soshu Ri
宗洙 李
和久 信一
Shinichi Waku
信一 和久
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Elmex Ltd
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Abstract

To provide a wiping inspection kit capable of draining the entire amount of an internal solution from a lid of an inverted container.SOLUTION: A wiping inspection kit 100 includes a container body 10, and a lid 20 detachably attached to the container body 10 and fixedly installing a cotton swab shaft 7 that is housed in the container body 10. The lid 20 includes: a mounting portion 21 connected to the container body 10; a hollow protrusion 25 that is connected to the mounting portion 21 and vertically installed on the outside of the container body 10; and a cutout section 27 that seals an open end 23b of a hollow portion of the hollow protrusion 25. The cotton swab shaft 7 is inserted into the hollow portion and fixed. An inner wall of the hollow portion and/or an outer wall of the cotton swab shaft 7 forms a gap 24 that communicates over the entire length of the hollow portion when the cotton swab shaft 7 is inserted into the hollow portion.SELECTED DRAWING: Figure 4

Description

本発明は、清浄度検査等に使用でき、反転した容器の蓋部から内溶液の全量を排出可能な拭き取り検査用キットに関する。 The present invention relates to a wiping inspection kit that can be used for cleanliness inspection and the like and can discharge the entire amount of the internal solution from the lid of the inverted container.

従来から、手指や機材、食品加工施設、医療施設などの清浄を評価するために清浄度検査が行われ、評価方法の一つとして拭取り培養法がある。拭き取り培養法に使用する器具として、容器本体に、拭き取りに使用する綿棒が固定されたキャップを装着するものがある(特許文献1、特許文献2、特許文献3、特許文献4、特許文献5)。予め容器本体に希釈液が収納され、綿棒で被検体の表面を拭き取った後に容器に密封して希釈液を撹拌すると、検体が希釈液中に均一に撹拌された検体液を調製することができる。 Conventionally, a cleanliness test has been performed to evaluate the cleanliness of fingers, equipment, food processing facilities, medical facilities, etc., and one of the evaluation methods is a wiping culture method. As an instrument used in the wiping culture method, there is an instrument in which a cap to which a cotton swab used for wiping is fixed is attached to the container body (Patent Document 1, Patent Document 2, Patent Document 3, Patent Document 4, Patent Document 5). .. The diluted solution is stored in the container body in advance, the surface of the subject is wiped off with a cotton swab, and then the container is sealed and the diluted solution is stirred to prepare a sample solution in which the sample is uniformly stirred in the diluted solution. ..

大腸菌や大腸菌群等の菌数を測定するには、検体液をフィルム培地や寒天培地などで培養する。ピペットを使用せずに検体液を取り出すため、反転した容器の蓋部から検体液を排出させる拭き取り検査用の器具もある(特許文献6、特許文献7、特許文献8)。例えば、特許文献6の拭き取り検査用キットでは、図7に示すように、キャップ20が、螺条を形成した外壁部22とこれに連続する頂壁部23と、前記頂壁部23を被覆する上蓋25とで構成されている。前記頂壁部23の中央に、下方に突出する中空筒状部24が形成され、中空筒状部24の両端が開口している。綿棒軸4は、中空筒状部24に挿入により固定される。中空筒状部24の内周側には、軸方向に延長する複数の突条27が形成されており、挿入された綿棒軸4と突条27との間に形成された空隙が連通路30となる。容器を反転してキャップ20を下方に向けると、容器内の検体液が、連通路30から開口を経て容器外に排出される仕組みとなっている。 To measure the number of Escherichia coli and coliform bacteria, the sample solution is cultured in a film medium or an agar medium. In order to take out the sample liquid without using a pipette, there is also a device for wiping inspection in which the sample liquid is discharged from the lid of the inverted container (Patent Document 6, Patent Document 7, Patent Document 8). For example, in the wiping inspection kit of Patent Document 6, as shown in FIG. 7, the cap 20 covers the outer wall portion 22 having a spiral, the top wall portion 23 continuous thereto, and the top wall portion 23. It is composed of an upper lid 25. A hollow tubular portion 24 projecting downward is formed in the center of the top wall portion 23, and both ends of the hollow tubular portion 24 are open. The cotton swab shaft 4 is fixed to the hollow tubular portion 24 by insertion. A plurality of ridges 27 extending in the axial direction are formed on the inner peripheral side of the hollow tubular portion 24, and a gap formed between the inserted cotton swab shaft 4 and the ridge 27 is a continuous passage 30. It becomes. When the container is inverted and the cap 20 is turned downward, the sample liquid in the container is discharged from the communication passage 30 through the opening to the outside of the container.

一方、微生物の検出および同定法として、RT−PCR法やリアルタイムRT−PCR法などの遺伝子検出法が普及しつつある。例えば、急性胃腸炎の主要な原因体としてノロウイルス等がある。ウイルスは、大腸菌などの細菌と相違して組織培養法で分離・検出することができないため、細菌培養に代えてウイルス遺伝子の検出を行っている。ウイルスの毒性に鑑みて、調製した検査液をピペットなどの器具を使用せず、反転した容器の蓋部から検体液を取り出せるものが好適である。 On the other hand, gene detection methods such as RT-PCR method and real-time RT-PCR method are becoming widespread as methods for detecting and identifying microorganisms. For example, norovirus is a major cause of acute gastroenteritis. Unlike bacteria such as Escherichia coli, viruses cannot be separated and detected by the tissue culture method, so virus genes are detected instead of bacterial culture. In view of the virulence of the virus, it is preferable that the prepared test solution can be taken out from the lid of the inverted container without using an instrument such as a pipette.

特開平10−229871号公報Japanese Unexamined Patent Publication No. 10-229871 特開2008−32541号公報Japanese Unexamined Patent Publication No. 2008-32541 特開2008−101971号公報Japanese Unexamined Patent Publication No. 2008-101971 特開2011−069778号公報Japanese Unexamined Patent Publication No. 2011-069778 特開2016−70820号公報Japanese Unexamined Patent Publication No. 2016-70820 特開2003−344232号公報Japanese Unexamined Patent Publication No. 2003-344232 特開2013−108803号公報Japanese Unexamined Patent Publication No. 2013-108803 特開2016−050911号公報Japanese Unexamined Patent Publication No. 2016-050911

微生物培養に使用される従来の拭き取り検査用器具は、通常10mlの希釈液が収納される(特許文献6)。微生物培養に使用される検査液が1mlであり、検査結果の正確を期すためデュープリケートで行い、容器に残存する検査液を保存すること、および検体液の調製の容易さなどを勘案し10ml程度が好ましい。一方、遺伝子検出法は、検体から抽出した核酸を材料として特定の遺伝子配列だけを増やす核酸増幅法であり、微生物培養と相違して短時間で結果が得られる特徴がある。PCR法の検体採取に従来の拭き取り検査用キットを使用すると、検体液に含まれる核酸量が不足し、迅速な検査が困難な場合がある。 A conventional wiping test instrument used for culturing microorganisms usually contains 10 ml of a diluted solution (Patent Document 6). The test solution used for microbial culture is 1 ml, and it is duplicated to ensure the accuracy of the test result, and the test solution remaining in the container is stored, and considering the ease of preparation of the sample solution, about 10 ml. Is preferable. On the other hand, the gene detection method is a nucleic acid amplification method in which only a specific gene sequence is increased using a nucleic acid extracted from a sample as a material, and is characterized in that results can be obtained in a short time unlike microbial culture. When a conventional wiping test kit is used for sample collection by the PCR method, the amount of nucleic acid contained in the sample solution may be insufficient, and rapid testing may be difficult.

また、検体濃度を10倍にするため、収納する希釈液量を1/10、例えば1mlに低減すると、蓋部から検体液が排出されないという問題が生じる。例えば、図7に示すキャップ20では、容器を反転すると、頂壁部23を底部とし、螺条を形成した外壁部22を外周とする空間に検体液が移動する。頂壁部23の直径は約20mmであり、中空筒状部24の直径は約6mmであるから、1mlの検体液の深さは約3.5mm程度となる。中空筒状部24は、綿棒軸4を固定するために頂壁部23から5〜10mm程度の長さで垂下しているため、検体液は中空筒状部24内の連通路30に導入できず、開口から容器外に排出させることができない。 Further, if the amount of the diluent to be stored is reduced to 1/10, for example, 1 ml in order to increase the sample concentration by 10 times, there arises a problem that the sample solution is not discharged from the lid. For example, in the cap 20 shown in FIG. 7, when the container is inverted, the sample liquid moves to a space having the top wall portion 23 as the bottom portion and the spiral outer wall portion 22 as the outer circumference. Since the diameter of the top wall portion 23 is about 20 mm and the diameter of the hollow tubular portion 24 is about 6 mm, the depth of 1 ml of the sample solution is about 3.5 mm. Since the hollow tubular portion 24 hangs down from the top wall portion 23 at a length of about 5 to 10 mm for fixing the cotton swab shaft 4, the sample liquid can be introduced into the communication passage 30 in the hollow tubular portion 24. It cannot be discharged from the container through the opening.

中空筒状部24は、綿棒軸4を固定するものであり、安定した固定のために所定の長さを必要とする。したがって、従来のキャップ構造では、キャップの径を小型化しても希釈液が残留し、収納した希釈液の全量を排出させることができない。 The hollow tubular portion 24 fixes the cotton swab shaft 4, and requires a predetermined length for stable fixing. Therefore, in the conventional cap structure, even if the diameter of the cap is reduced, the diluent remains, and the entire amount of the stored diluent cannot be discharged.

上記現状に鑑み、本発明は、内容量が少量であっても、容器を反転させて蓋部から内容量の全量を排出させることができる拭き取り検査用キットを提供することを目的とする。 In view of the above situation, it is an object of the present invention to provide a wiping inspection kit capable of inverting a container and discharging the entire amount of the content from the lid even if the content is small.

本発明者らは、拭き取り検査用キットについて詳細に検討した結果、蓋部を、容器本体への装着部と、容器本体の外方に垂設される中空突出部と、この中空突出部の一方の開口端を密封する切り取り切片とで構成すると、綿棒軸を中空突出部の中空部に挿入して安定に固定できること、中空部と綿棒軸との接触部に間隙を形成すれば中空部内に流通路を形成できること、切り取り切片を除去することで、容器本体の検体液の全量を前記流通路を経て開口端から排出できることを見出し、本発明を完成させた。 As a result of detailed examination of the wiping inspection kit, the present inventors have attached the lid to the container body, a hollow protrusion vertically provided on the outside of the container body, and one of the hollow protrusions. If it is composed of a cut piece that seals the open end, the cotton swab shaft can be inserted into the hollow part of the hollow protrusion and fixed stably, and if a gap is formed between the hollow part and the cotton swab shaft, it will flow into the hollow part. The present invention has been completed by finding that a path can be formed and that the entire amount of the sample liquid in the container body can be discharged from the open end through the flow passage by removing the cut section.

すなわち、本発明は、容器本体と、前記容器本体に収納される綿棒軸を固設し、かつ前記容器本体に脱着可能に装着される蓋部とを含む拭き取り検査用キットであって、
前記蓋部は、前記容器本体への装着部と、前記装着部に連設され、前記容器本体の外方に垂設される中空突出部と、前記中空突出部の中空部の開口端を密封する切り取り切片とからなり、
前記綿棒軸は、前記中空部に挿入して固設され、
前記中空部の内壁および/または前記綿棒軸の外壁は、前記中空部に前記綿棒軸を挿入した際に前記中空部の全長に亘って連通する間隙を形成するものであることを特徴とする、拭き取り検査用キットを提供するものである。
That is, the present invention is a wiping inspection kit including a container body and a lid portion in which a cotton swab shaft housed in the container body is fixedly attached and detachably attached to the container body.
The lid portion seals a mounting portion to the container body, a hollow protruding portion connected to the mounting portion and vertically hung on the outside of the container body, and an open end of the hollow portion of the hollow protruding portion. Consists of a cut section
The cotton swab shaft is inserted into the hollow portion and fixed.
The inner wall of the hollow portion and / or the outer wall of the cotton swab shaft form a gap that communicates over the entire length of the hollow portion when the cotton swab shaft is inserted into the hollow portion. It provides a wiping inspection kit.

また本発明は、前記中空部は、前記中空突出部の開口端から前記切り取り切片に向かって断面積S1の中空部T1と断面積S2の中空部T2とで構成され、前記断面積S1>前記断面積S2であることを特徴とする、前記拭き取り検査用キットを提供するものである。 Further, in the present invention, the hollow portion is composed of a hollow portion T1 having a cross section S1 and a hollow portion T2 having a cross section S2 from the open end of the hollow protrusion toward the cut section, and the cross section S1> said. The present invention provides the wiping inspection kit, which is characterized by having a cross section S2.

また本発明は、前記綿棒軸は、軸の長手方向に形成された複数の凸状部を有し、
前記綿棒軸が挿入される前記中空部T1および/または前記中空部T2は断面視多形であることを特徴とする、前記拭き取り検査用キットを提供するものである。
Further, in the present invention, the cotton swab shaft has a plurality of convex portions formed in the longitudinal direction of the shaft.
Provided is the wiping inspection kit, characterized in that the hollow portion T1 and / or the hollow portion T2 into which the cotton swab shaft is inserted is polymorphic in cross section.

また本発明は、前記綿棒軸が挿入される前記中空部T1および/または前記中空部T2は、断面視円形または角が丸く成形された方形であることを特徴とする、前記拭き取り検査用キットを提供するものである。 The present invention also relates to the wiping inspection kit, wherein the hollow portion T1 and / or the hollow portion T2 into which the cotton swab shaft is inserted is a square formed with a circular cross section or rounded corners. It is to provide.

また本発明は、前記中空突出部は、外周に、折り曲げ切片を嵌合できる凹部が形成されることを特徴とする、前記拭き取り検査用キットを提供するものである。 The present invention also provides the wiping inspection kit, wherein the hollow protrusion is formed with a recess on the outer periphery into which a bent section can be fitted.

また本発明は、前記蓋部は、前記切り取り切片の外周に、コンタミ防止用突起を有することを特徴とする、前記拭き取り検査用キットを提供するものである。 The present invention also provides the wiping inspection kit, characterized in that the lid portion has a contamination prevention protrusion on the outer periphery of the cut section.

また、前記容器本体の底面部は、前記容器本体の外方に延設される翼が形成された平板であり、自立可能であることを特徴とする、前記拭き取り検査用キットを提供するものである。 Further, the bottom surface portion of the container body is a flat plate on which wings extending to the outside of the container body are formed, and is characterized in that it can stand on its own, and provides the wiping inspection kit. is there.

本発明の拭き取り検査用キットは、容器を反転すると蓋部から検体液の全量を排出させることができる。 The wiping test kit of the present invention can discharge the entire amount of the sample liquid from the lid when the container is inverted.

図1(A)は、本発明の拭き取り検査用キットの正面図であり、図1(B)は、側面図であり、図1(C)は、縦断斜視面図である。1 (A) is a front view of the wiping inspection kit of the present invention, FIG. 1 (B) is a side view, and FIG. 1 (C) is a longitudinal perspective view. 図2(A)は、拭き取り検査用キットを構成する容器本体と蓋部の正面図であり、図2(B)は、これらの縦断面図である。FIG. 2A is a front view of the container body and the lid portion constituting the wiping inspection kit, and FIG. 2B is a vertical cross-sectional view of these. 図3(A)は、蓋部を説明する図であり、図3(B)は蓋部の底面図であり、図3(C)および図3(D)は、中空部と綿棒軸との関係を説明する図である。また、図3(E)は、切り取り切片を除去した後に拭き取り検査用キットを反転し、検体液が流出できることを説明する図であり、図3(F)は、切り取り切片を除去した後の蓋部の平面図である。FIG. 3A is a view for explaining the lid portion, FIG. 3B is a bottom view of the lid portion, and FIGS. 3C and 3D are a hollow portion and a cotton swab shaft. It is a figure explaining the relationship. Further, FIG. 3 (E) is a diagram for explaining that the sample solution can flow out by inverting the wiping test kit after removing the cut section, and FIG. 3 (F) shows a lid after removing the cut section. It is a plan view of a part. 図4(A)は、拭き取り検査用キットを構成する容器本体と蓋部の側面図であり、図4(B)は綿棒軸を固定した蓋部と容器本体の縦断面図であり、図4(C)は、希釈液を収納した拭き取り検査用キットの縦断面図である。FIG. 4A is a side view of the container body and the lid portion constituting the wiping inspection kit, and FIG. 4B is a vertical cross-sectional view of the lid portion and the container body to which the cotton swab shaft is fixed. (C) is a vertical cross-sectional view of a wiping inspection kit containing the diluent. 図5は、蓋部の斜視図である。FIG. 5 is a perspective view of the lid portion. 図6(A)は、拭き取り検査用キットの正面図であり、図6(B)は側面図であり、図6(C)は、蓋部の凹部に他の拭き取り検査用キットの切り取り切片を挿入した状態を説明する図である。FIG. 6 (A) is a front view of the wiping inspection kit, FIG. 6 (B) is a side view, and FIG. 6 (C) shows a cut section of another wiping inspection kit in the recess of the lid. It is a figure explaining the inserted state. 図7は、従来の拭き取り検査用キットの蓋部の一例を説明する図である。FIG. 7 is a diagram illustrating an example of a lid portion of a conventional wiping inspection kit.

本発明は、容器本体と、前記容器本体に収納される綿棒軸を固設し、かつ前記容器本体に脱着可能に装着される蓋部とを含む拭き取り検査用キットであって、
前記蓋部は、前記容器本体への装着部と、前記装着部に連設され、前記容器本体の外方に垂設される中空突出部と、前記中空突出部の中空部の開口端を密封する切り取り切片とからなり、
前記綿棒軸は、前記中空部に挿入して固設され、
前記中空部の内壁および/または前記綿棒軸の外壁は、前記中空部に前記綿棒軸を挿入した際に前記中空部の全長に亘って連通する間隙を形成するものであることを特徴とする、拭き取り検査用キットである。以下、図面を参照して本発明を詳細に説明する。
The present invention is a wiping inspection kit including a container body and a lid portion to which a cotton swab shaft housed in the container body is fixedly attached and detachably attached to the container body.
The lid portion seals a mounting portion to the container body, a hollow protruding portion connected to the mounting portion and vertically hung on the outside of the container body, and an open end of the hollow portion of the hollow protruding portion. Consists of a cut section
The cotton swab shaft is inserted into the hollow portion and fixed.
The inner wall of the hollow portion and / or the outer wall of the cotton swab shaft form a gap that communicates over the entire length of the hollow portion when the cotton swab shaft is inserted into the hollow portion. It is a wiping inspection kit. Hereinafter, the present invention will be described in detail with reference to the drawings.

本発明の拭き取り検査用キットの好適な実施態様の一例として、容器本体10に、蓋部20が脱着可能に装着された拭き取り検査用キット100の正面図を図1(A)に、側面図を図1(B)に示す。容器本体10は、正面よりも側面がやや幅狭く構成された横断面視略楕円の筒状である。容器本体10の開口部1に蓋部20の装着部21を嵌着すると容器本体10に蓋部20を装着することができる。蓋部20は、容器本体10の外方に垂設される中空突出部25と切り取り切片27とを装着部21と一体に有する。図1(C)は拭き取り検査用キット100の縦断面斜視図である。蓋部20の中空突出部25には、容器本体10と連通する中空部23が形成され、中空部23には、拭き取り部8を有する綿棒軸7が挿入されている。なお、中空部23の他端は、切り取り切片27によって密封されている。 As an example of a preferred embodiment of the wiping inspection kit of the present invention, a front view of the wiping inspection kit 100 in which the lid 20 is detachably attached to the container body 10 is shown in FIG. 1 (A), and a side view is shown in FIG. It is shown in FIG. 1 (B). The container body 10 has a tubular shape having a substantially elliptical cross-sectional view, which is configured so that the side surface is slightly narrower than the front surface. When the mounting portion 21 of the lid portion 20 is fitted into the opening 1 of the container body 10, the lid portion 20 can be mounted on the container body 10. The lid portion 20 has a hollow protruding portion 25 vertically hung on the outside of the container body 10 and a cut section 27 integrally with the mounting portion 21. FIG. 1C is a vertical cross-sectional perspective view of the wiping inspection kit 100. A hollow portion 23 communicating with the container body 10 is formed in the hollow protruding portion 25 of the lid portion 20, and a cotton swab shaft 7 having a wiping portion 8 is inserted into the hollow portion 23. The other end of the hollow portion 23 is sealed by the cut section 27.

容器本体10の素材は、高密度ポリエチレン、低密度ポリエチレン等の圧縮変形可能な軟質材料である。また、蓋部は、ABS、PS、PP等が好適である。 The material of the container body 10 is a soft material that can be compressed and deformed, such as high-density polyethylene and low-density polyethylene. Further, ABS, PS, PP or the like is suitable for the lid portion.

本発明で使用する蓋部20は、装着部21と中空突出部25と切り取り切片27とが一体に形成されたことを特徴とする。図2(A)に容器本体10および蓋部20の正面図を、図2(B)にこれらの縦断面図を示す。装着部21の形状は、容器本体10の開口部1の形状に対応して適宜選択することができる。図2(A)に示す容器本体10の開口部1は筒状であるため、蓋部20の装着部21も前記筒状に嵌着できる筒状となっている。しかしながら、本発明ではこれに限定されず、例えば開口部1の外周に螺条を形成した場合には、装着部21にもこれに対応する螺条を形成するものであってもよい。開口部1と装着部21とを螺着させ、蓋部20を容器本体10に装着することができ、および螺条を逆方向に回転することで蓋部20を離脱することができる。なお、中空突出部25は、中空部23が装着部21より容器本体10の外方に形成されることを条件に、装着部21との結合部より容器本体10側に向かう部分を有していてもよい。例えば、図2(B)に、中空突出部25の一部として、装着部21と平行する軒部22が形成される態様を示す。軒部22により、開口部1と装着部21との嵌着部から物質が混入するのを回避することができる。なお、図2(A)の符号28aは、軽量化を目的として削られた軽量化用凹部である。 The lid portion 20 used in the present invention is characterized in that the mounting portion 21, the hollow protruding portion 25, and the cut section 27 are integrally formed. FIG. 2 (A) shows a front view of the container body 10 and the lid portion 20, and FIG. 2 (B) shows a vertical cross-sectional view of these. The shape of the mounting portion 21 can be appropriately selected according to the shape of the opening 1 of the container body 10. Since the opening 1 of the container body 10 shown in FIG. 2A has a tubular shape, the mounting portion 21 of the lid portion 20 also has a tubular shape that can be fitted into the tubular shape. However, the present invention is not limited to this, and for example, when a screw is formed on the outer periphery of the opening 1, the mounting portion 21 may also be formed with a corresponding screw. The opening 1 and the mounting portion 21 can be screwed together to mount the lid portion 20 on the container body 10, and the lid portion 20 can be detached by rotating the screw in the opposite direction. The hollow projecting portion 25 has a portion that faces the container body 10 side from the joint portion with the mounting portion 21 on the condition that the hollow portion 23 is formed outside the container body 10 from the mounting portion 21. You may. For example, FIG. 2B shows an embodiment in which an eaves portion 22 parallel to the mounting portion 21 is formed as a part of the hollow protrusion portion 25. The eaves portion 22 makes it possible to prevent substances from being mixed in from the fitting portion between the opening portion 1 and the mounting portion 21. Reference numeral 28a in FIG. 2A is a weight reduction recess cut for the purpose of weight reduction.

図2(B)に示すように、中空突出部25には、容器本体10側の開口23aから切り取り切片27側の端部23bに連通する中空部23が形成され、開口23aは解放端であり容器本体10と連通する。中空部23は、綿棒軸7を固定する機能と、容器本体10の検体液を蓋部20から排出させるための流通路を形成する機能とを有する。開口23aから図示しない綿棒軸7を挿入すると、綿棒軸7を蓋部20に固定することができる。したがって、中空部23の長さは綿棒軸7を安定して固定できる長さとする。なお、端部23bは切り取り切片27で密封されている。 As shown in FIG. 2B, the hollow projecting portion 25 is formed with a hollow portion 23 communicating from the opening 23a on the container body 10 side to the end portion 23b on the cut section 27 side, and the opening 23a is an open end. Communicates with the container body 10. The hollow portion 23 has a function of fixing the cotton swab shaft 7 and a function of forming a flow passage for discharging the sample liquid of the container body 10 from the lid portion 20. By inserting the cotton swab shaft 7 (not shown) from the opening 23a, the cotton swab shaft 7 can be fixed to the lid portion 20. Therefore, the length of the hollow portion 23 is set so that the cotton swab shaft 7 can be stably fixed. The end portion 23b is sealed with a cut section 27.

中空部23は、全長にわたり同径であってもよいが、図3(A)に示すように、断面積の異なる中空部23T1と中空部23T2とで構成されるものであってもよい。例えば、中空部23T1の断面積をS1、中空部23T2の断面積をS2とした場合に断面積S1>断面積S2となるように設計すると、異なる太さや形状の綿棒軸7を中空部23で固設することができる。この際、中空部23T1および中空部23T2の長さは、それぞれ、綿棒軸7を安定して固定できる長さとする。 The hollow portion 23 may have the same diameter over the entire length, but as shown in FIG. 3A, the hollow portion 23 may be composed of the hollow portion 23T1 and the hollow portion 23T2 having different cross sections. For example, when the cross section of the hollow portion 23T1 is S1 and the cross section of the hollow portion 23T2 is S2, if the cross section is designed to be S1> cross section S2, the cotton swab shafts 7 having different thicknesses and shapes are formed in the hollow portion 23. Can be fixed. At this time, the lengths of the hollow portion 23T1 and the hollow portion 23T2 are set to lengths that can stably fix the cotton swab shaft 7, respectively.

中空部23の内壁と綿棒軸7の外壁は、中空部23に綿棒軸7を挿入した際に中空部23の全長を連通する間隙24が形成されることを要する。この間隙24が検体液の流通路となる。図3(B)に蓋部20の底面図の一例を示す。この蓋部20は、中央に方形の中空部23T2と、中空部23T2の外周に、角が丸く成形された方形の中空部23T1とが形成される態様となっている。図3(C)に中空部23T2に固定する円柱状の綿棒軸7の横断面図を、図3(D)に中空部23T1に固定する軸の長手方向の4か所に凹部7aを形成した多形の綿棒軸7の横断面図を示す。図3(D)に示すように、角が丸く成形された方形の中空部23T1に多形の綿棒軸7を挿入すると、綿棒軸7の4つの凹部7aを中空部23の全長に亘る間隙24、すなわち流通路とすることができる。また、中空部23T1と綿棒軸7との接触面が大きいため、綿棒軸7を安定して固定することができる。なお、角が丸く成形された方形の中空部23T1に円柱状の綿棒軸7(図3(C)参照)を挿入すれば、前記方形の角部が間隙24となり、中空部23の全長に亘る間隙24を形成することができる。上記は一例である。中空部23T1や中空部23T2の内壁と綿棒軸7の外周の双方に凹凸加工し、綿棒軸7の固定と間隙24の形成による流通路の確保とを同時に行う態様等であってもよい。 The inner wall of the hollow portion 23 and the outer wall of the cotton swab shaft 7 are required to form a gap 24 that communicates the entire length of the hollow portion 23 when the cotton swab shaft 7 is inserted into the hollow portion 23. This gap 24 serves as a flow path for the sample liquid. FIG. 3B shows an example of a bottom view of the lid portion 20. The lid portion 20 has an embodiment in which a square hollow portion 23T2 is formed in the center and a square hollow portion 23T1 having rounded corners is formed on the outer periphery of the hollow portion 23T2. FIG. 3C shows a cross-sectional view of the columnar cotton swab shaft 7 fixed to the hollow portion 23T2, and FIG. 3D shows recesses 7a formed at four locations in the longitudinal direction of the shaft fixed to the hollow portion 23T1. The cross-sectional view of the polymorphic cotton swab shaft 7 is shown. As shown in FIG. 3D, when the polymorphic cotton swab shaft 7 is inserted into the square hollow portion 23T1 formed with rounded corners, the four recesses 7a of the cotton swab shaft 7 are inserted into the gap 24 over the entire length of the hollow portion 23. That is, it can be a flow passage. Further, since the contact surface between the hollow portion 23T1 and the cotton swab shaft 7 is large, the cotton swab shaft 7 can be stably fixed. If a columnar cotton swab shaft 7 (see FIG. 3C) is inserted into the square hollow portion 23T1 having rounded corners, the square corner becomes a gap 24 and extends over the entire length of the hollow portion 23. A gap 24 can be formed. The above is an example. The inner wall of the hollow portion 23T1 or the hollow portion 23T2 and the outer periphery of the cotton swab shaft 7 may be unevenly processed so that the cotton swab shaft 7 is fixed and the flow passage is secured by forming the gap 24 at the same time.

中空部23の端部23bは切り取り切片27で密封されている(図3(A)参照)。切り取り切片27を把持して応力を負荷すると切り取り切片27を切り離すことができ、中空部23T2の封止端が解放され端部23bが開口する。切り取り切片27を除去した後に、蓋部20が下方になるように拭き取り検査用キット100を反転すると、容器本体10に収納された希釈液4が、装着部21と中空突出部25とによって構成される空間に移動し、中空突出部25の開口23aから中空部23に導入され、中空部23に形成された間隙24を経て開口した端部23bから排出される(図3(E))。図3(F)に切り取り切片27を除去した蓋部20の平面図を示す。中空突出部25の中央に端部23bが開口している。 The end portion 23b of the hollow portion 23 is sealed with a cut section 27 (see FIG. 3 (A)). When the cut section 27 is gripped and stress is applied, the cut section 27 can be separated, the sealed end of the hollow portion 23T2 is released, and the end portion 23b opens. After removing the cut section 27, when the wiping inspection kit 100 is inverted so that the lid portion 20 is downward, the diluent 4 stored in the container body 10 is composed of the mounting portion 21 and the hollow protrusion 25. It is introduced into the hollow portion 23 from the opening 23a of the hollow protrusion 25, and is discharged from the end portion 23b opened through the gap 24 formed in the hollow portion 23 (FIG. 3 (E)). FIG. 3 (F) shows a plan view of the lid portion 20 from which the cut section 27 has been removed. The end 23b is open in the center of the hollow protrusion 25.

図4(A)に容器本体10および蓋部20の側面図を示す。容器本体10のほぼ中央には容器本体10の長手方向に延びる楕円形の凹部3が形成され、凹部3には、容器本体10の幅方向に平行する複数の凸条2が形成され、容器本体10を容易に押圧できる構成となっている。また、図4(B)に容器本体10の縦断面図と、中空部23T2に綿棒軸7を挿入した蓋部20の縦断面図を、図4(C)に希釈液4を収納した図4(B)の拭き取り検査用キット100の縦断面図を示す。綿棒軸7は、中空部23T2に挿入して固定されている。拭き取り部8で被検体を拭き取る際に、凹部3を押圧して拭き取り部8に含浸される希釈液4の液量を調整することができる。中空部23T2の内壁と綿棒軸7との間に間隙24が形成され、この間隙24は開口23aから端部23bまで連通している。切り取り切片27を切り離すと中空部23T2の端部23bが開口し、希釈液4の全量を排出することができる。 FIG. 4A shows a side view of the container body 10 and the lid 20. An elliptical recess 3 extending in the longitudinal direction of the container body 10 is formed substantially in the center of the container body 10, and a plurality of protrusions 2 parallel to the width direction of the container body 10 are formed in the recess 3, and the container body is formed. The structure is such that 10 can be easily pressed. Further, FIG. 4B shows a vertical cross-sectional view of the container body 10, a vertical cross-sectional view of the lid 20 in which the cotton swab shaft 7 is inserted into the hollow portion 23T2, and FIG. 4C shows the diluent 4 in which the diluent 4 is stored. The vertical sectional view of the wiping inspection kit 100 of (B) is shown. The cotton swab shaft 7 is inserted into and fixed to the hollow portion 23T2. When wiping the subject with the wiping portion 8, the concave portion 3 can be pressed to adjust the amount of the diluted solution 4 impregnated in the wiping portion 8. A gap 24 is formed between the inner wall of the hollow portion 23T2 and the cotton swab shaft 7, and the gap 24 communicates from the opening 23a to the end 23b. When the cut section 27 is cut off, the end portion 23b of the hollow portion 23T2 is opened, and the entire amount of the diluent 4 can be discharged.

蓋部20の中空突出部25は、中空部23を有すれば外周の形状に特に限定はない。図5に蓋部20の一例の斜視図を示す。符号28aは軽量化用凹部であり、符号28bは、他の拭き取り検査用キット100の切り取り切片27を嵌着して切り取るための嵌着用凹部であり、符号27aはコンタミを防止するためのコンタミ防止用突起である。嵌着用凹部28bは、切り取り切片27近傍から装着部21近傍に至る略楕円形で構成されることが好ましい。図6(A)に、拭き取り検査用キット100の正面図、図6(B)に側面図を示し、図6(C)に、二つの拭き取り検査用キット100の蓋部20どうしを90度の角度で対抗させ、一方の拭き取り検査用キット100の嵌着用凹部28bに、他の拭き取り検査用キット100の切り取り切片27を挿入した状態を示す。一方の切り取り切片27を他方の嵌着用凹部28bで固定し折り曲げると、切り取り切片27を容易に切り離すことができる。嵌着用凹部28bは、このような切り取り切片27を簡便に切り離すための機構として使用することができる。その際、コンタミ防止用突起27aが存在すると、端部23bと切除される切り取り切片27との間にコンタミ防止用突起27aの高さの間隙が形成される。開口された端部23bが他の拭き取り検査用キット100のいずれの部分とも接触しないため、端部23bから微生物が混入することを防止することができる。 The shape of the outer periphery of the hollow protruding portion 25 of the lid portion 20 is not particularly limited as long as it has the hollow portion 23. FIG. 5 shows a perspective view of an example of the lid portion 20. Reference numeral 28a is a recess for weight reduction, reference numeral 28b is a fitting recess for fitting and cutting a cut section 27 of another wiping inspection kit 100, and reference numeral 27a is a contamination prevention for preventing contamination. It is a protrusion. The fitting recess 28b is preferably formed in a substantially elliptical shape extending from the vicinity of the cut section 27 to the vicinity of the mounting portion 21. FIG. 6A shows a front view of the wiping inspection kit 100, FIG. 6B shows a side view, and FIG. 6C shows two lids 20 of the wiping inspection kit 100 at 90 degrees. It shows a state in which the cut section 27 of the other wiping inspection kit 100 is inserted into the fitting recess 28b of one wiping inspection kit 100 so as to oppose each other at an angle. When one cut section 27 is fixed by the other fitting recess 28b and bent, the cut section 27 can be easily separated. The fitting recess 28b can be used as a mechanism for easily separating such a cut section 27. At that time, if the contamination prevention protrusion 27a is present, a gap at the height of the contamination prevention protrusion 27a is formed between the end portion 23b and the cut section 27 to be cut off. Since the opened end portion 23b does not come into contact with any portion of the other wiping inspection kit 100, it is possible to prevent microorganisms from being mixed in from the end portion 23b.

拭き取り検査用キット100の外形は、例えば図6(A)、(B)に示すように、容器本体10は、正面の幅が側面の幅より長く形成され、その横断面は略楕円形である。一方、容器本体の底面部5を楕円に限定せず、容器本体10から突出する翼6を有する円盤状としてもよい。これにより、底面部5を机上に載置すると、拭き取り検査用キット100を自立させることができる。 As shown in FIGS. 6A and 6B, for example, the outer shape of the wiping inspection kit 100 is such that the width of the front surface of the container body 10 is longer than the width of the side surface, and the cross section thereof is substantially elliptical. .. On the other hand, the bottom surface portion 5 of the container body is not limited to an ellipse, and may be a disk shape having wings 6 protruding from the container body 10. As a result, when the bottom surface portion 5 is placed on the desk, the wiping inspection kit 100 can be made to stand on its own.

拭き取り検査用キット100に使用する綿棒は、綿棒軸7が間隙24を形成できればよく、他の条件は従来公知のものを使用することができる。例えば、材質は、拭き取り時に力を加えても折れにくい可撓性樹脂等を好適に使用する。同様に、拭き取り部8の形状や素材も、拭き取り箇所や検体液の分析方法によって適宜選択することができる。したがって、容器本体10に収納できることを条件に、形状は、球形でも板状でもよい。また、拭き取り部8の素材は、綿などの天然物の他、ポリエステル、ポリアミドなどの合成樹脂やガラスウールなどであってもよい。これら素材は、繊維状の他、スポンジ状、その他、任意に選択することができる。特に、検体液をPCR法で分析する場合には、拭取り部8に由来する核酸が含まれていないことが好ましい。拭き取り部8が合成樹脂であれば、熱溶着、合成樹脂系接着剤などを介して綿棒軸7に拭き取り部8を固定することが好ましい。 As the cotton swab used in the wiping inspection kit 100, it is sufficient that the cotton swab shaft 7 can form a gap 24, and other conventionally known conditions can be used. For example, as the material, a flexible resin or the like that is hard to break even when a force is applied during wiping is preferably used. Similarly, the shape and material of the wiping portion 8 can be appropriately selected depending on the wiping portion and the analysis method of the sample liquid. Therefore, the shape may be spherical or plate-like, provided that it can be stored in the container body 10. Further, the material of the wiping portion 8 may be a natural product such as cotton, a synthetic resin such as polyester or polyamide, or glass wool. These materials may be fibrous, sponge-like, or any other material. In particular, when the sample solution is analyzed by the PCR method, it is preferable that the nucleic acid derived from the wiping portion 8 is not contained. If the wiping portion 8 is a synthetic resin, it is preferable to fix the wiping portion 8 to the cotton swab shaft 7 via heat welding, a synthetic resin adhesive, or the like.

容器本体10には、あらかじめ所定量の希釈液4を収容することができる。拭き取り検査用キットの使用目的に応じて希釈液4の種類を選択することができ、例えば、精製水、生理食塩水、リン酸緩衝生理食塩水、緩衝ペプトン水等を例示することができる。希釈液量は、拭き取り検査用キット100から排出した際の液量が0.5〜3mlとなることが好ましく、より好ましくは1〜2mlである。本発明の拭き取り検査用キット100は、希釈液4の全量を排出することができるため、希釈液量を少なくして高濃度の検体液を調製することができる。 A predetermined amount of the diluent 4 can be stored in the container body 10 in advance. The type of the diluent 4 can be selected according to the purpose of use of the wiping test kit, and examples thereof include purified water, physiological saline, phosphate buffered saline, and buffered peptone water. The amount of the diluted solution is preferably 0.5 to 3 ml, more preferably 1 to 2 ml, when discharged from the wiping inspection kit 100. Since the wiping test kit 100 of the present invention can discharge the entire amount of the diluent 4, it is possible to prepare a high-concentration sample solution by reducing the amount of the diluent.

拭き取り試料としては、食品工場、飲料工場、半導体製造工場、その他の環境中より採取した拭き取り試料の他、拭き取り部8をペットや家畜の飼料、医薬品、土壌、上下水、生体組織、生体排出物に接触して得た試料等であってもよい。 As wiping samples, in addition to wiping samples collected from food factories, beverage factories, semiconductor manufacturing factories, and other environments, the wiping section 8 is used as feed for pets and livestock, pharmaceuticals, soil, water and sewage, biological tissues, and biological waste products. It may be a sample or the like obtained by contacting with.

本発明の拭き取り検査用キット100は、拭き取り試料を使用して、培養法による大腸菌やサルモネラなどの食中毒菌の検出に使用できる。より好ましくは、拭き取り試料からDNAやRNAなどの核酸を抽出し、ポリメラーゼ連鎖反応(PCR)により核酸を増幅することを特徴とする、遺伝子検出法による検出である。例えば、PCR(Polymerase Chain Reaction)によりノロウイルスやロタウイルスなどに特異な核酸を増幅し、リアルタイムPCR装置、RT−PCR(Reverse Transcription−PCR)、RT−LAMP(Reverse Transcription−Loop−mediated Isothermal Amplification)等の測定器を用いて、食中毒原因ウイルスを検出することができる。また、検出目的のウイルスの抗体を利用した抗原検出法(ELISA法や、BLEIA法、イムノクロマト法)を導入し、免疫学的手法によって食中毒原因ウイルスを検出することもできる。本発明の拭き取り検査用キット100は、高濃度の検体液を調製できるため、遺伝子検査法や免疫学的手法により、短時間に検出結果を得ることができる。 The wiping test kit 100 of the present invention can be used for detecting food poisoning bacteria such as Escherichia coli and Salmonella by a culture method using a wiping sample. More preferably, the detection by a gene detection method is characterized in that nucleic acids such as DNA and RNA are extracted from the wiped sample and the nucleic acids are amplified by a polymerase chain reaction (PCR). For example, a nucleic acid specific to norovirus or rotavirus is amplified by PCR (Polymerase Chain Reaction), and a real-time PCR device, RT-PCR (Reverse Translation-PCR), RT-LAMP (Reverse Translation-Loop-mediated Analysis, etc.) is used. The virus that causes food poisoning can be detected using the measuring instrument of. It is also possible to introduce an antigen detection method (ELISA method, BLEIA method, immunochromatography method) using an antibody of the virus for detection, and detect the food poisoning-causing virus by an immunological method. Since the wiping test kit 100 of the present invention can prepare a high-concentration sample solution, a detection result can be obtained in a short time by a genetic test method or an immunological method.

更に、本発明の拭き取り検査用キット100は、ATP拭き取り検査に使用することもできる。全ての生物にはATP(アデノシン三リン酸)が含まれるため、拭き取り試料からATPを抽出し、所定の試薬で発光させ、その発光量(Relative Light Unit;RLU)を測定し、拭き取り面の汚染を評価する方法である。発光量の多寡が拭取り面の生物量と比例するため、発光量をモニタリングして衛生管理を行うことができる。 Furthermore, the wiping inspection kit 100 of the present invention can also be used for ATP wiping inspection. Since all organisms contain ATP (adenosine triphosphate), ATP is extracted from the wiped sample, emitted with a predetermined reagent, the amount of emitted light (Relative Light Unit; RLU) is measured, and the wiped surface is contaminated. Is a method of evaluating. Since the amount of light emitted is proportional to the amount of biological material on the wiped surface, it is possible to monitor the amount of light emitted and perform hygiene management.

本発明の拭き取り検査用キット100の用途は、清浄度検査に限定されるものではない。例えば、拭き取り試料を遺伝子検査法で評価する際に、PCRで増幅する核酸を小麦、蕎麦、ラッカセイ、エビ、カニなどのアレルギー物質に変更すれば、アレルゲン検査に使用することができる。 The application of the wiping inspection kit 100 of the present invention is not limited to the cleanliness inspection. For example, when a wiped sample is evaluated by a genetic test method, if the nucleic acid amplified by PCR is changed to an allergen such as wheat, soba, peanut, shrimp, or crab, it can be used for allergen test.

本発明の拭き取り検査用キットは、微生物検査を簡便かつ迅速に行えるため、有用である。 The wiping test kit of the present invention is useful because it can easily and quickly perform a microbiological test.

1・・・開口部、
4・・・希釈液、
5・・・底面部、
7・・・綿棒軸、
8・・・拭き取り部、
10・・・容器本体、
20・・・蓋部、
21・・・装着部、
23、23T1、23T2・・・中空部、
24・・・間隙、
25・・・中空突出部、
27・・・切り取り切片、
27a・・・コンタミ防止用突起、
28a・・・軽量化用凹部、
28b・・・嵌着用凹部、
100・・・拭き取り検査用キット

1 ... opening,
4 ... Diluted solution,
5 ... Bottom part,
7 ... Cotton swab shaft,
8 ... Wiping part,
10 ... Container body,
20 ... lid,
21 ... Mounting part,
23, 23T1, 23T2 ... Hollow part,
24 ... Gap,
25 ... Hollow protrusion,
27 ... Cut section,
27a ... Contamination prevention protrusion,
28a ・ ・ ・ Recess for weight reduction,
28b ... Fitting recess,
100 ... Wiping inspection kit

Claims (7)

容器本体と、前記容器本体に収納される綿棒軸を固設し、かつ前記容器本体に脱着可能に装着される蓋部とを含む拭き取り検査用キットであって、
前記蓋部は、前記容器本体への装着部と、前記装着部に連設され、前記容器本体の外方に垂設される中空突出部と、前記中空突出部の中空部の開口端を密封する切り取り切片とからなり、
前記綿棒軸は、前記中空部に挿入して固設され、
前記中空部の内壁および/または前記綿棒軸の外壁は、前記中空部に前記綿棒軸を挿入した際に前記中空部の全長に亘って連通する間隙を形成するものであることを特徴とする、拭き取り検査用キット。
A wiping inspection kit including a container body and a lid portion to which a cotton swab shaft to be stored in the container body is fixedly attached and detachably attached to the container body.
The lid portion seals a mounting portion to the container body, a hollow protruding portion connected to the mounting portion and vertically hung on the outside of the container body, and an open end of the hollow portion of the hollow protruding portion. Consists of a cut section
The cotton swab shaft is inserted into the hollow portion and fixed.
The inner wall of the hollow portion and / or the outer wall of the cotton swab shaft form a gap that communicates over the entire length of the hollow portion when the cotton swab shaft is inserted into the hollow portion. Wiping inspection kit.
前記中空部は、前記中空突出部の開口端から前記切り取り切片に向かって断面積S1の中空部T1と断面積S2の中空部T2とで構成され、前記断面積S1>前記断面積S2であることを特徴とする、請求項1記載の拭き取り検査用キット。 The hollow portion is composed of a hollow portion T1 having a cross section S1 and a hollow portion T2 having a cross section S2 from the open end of the hollow protrusion toward the cut section, and the cross section S1> the cross section S2. The wiping inspection kit according to claim 1, wherein the kit is characterized in that. 前記綿棒軸は、軸の長手方向に形成された複数の凸状部を有し、
前記綿棒軸が挿入される前記中空部T1および/または前記中空部T2は断面視多形であることを特徴とする、請求項2記載の拭き取り検査用キット。
The cotton swab shaft has a plurality of convex portions formed in the longitudinal direction of the shaft, and has a plurality of convex portions.
The wiping inspection kit according to claim 2, wherein the hollow portion T1 and / or the hollow portion T2 into which the cotton swab shaft is inserted is polymorphic in cross section.
前記綿棒軸が挿入される前記中空部T1および/または前記中空部T2は、断面視円形または角が丸く成形された方形であることを特徴とする、請求項2記載の拭き取り検査用キット。 The wiping inspection kit according to claim 2, wherein the hollow portion T1 and / or the hollow portion T2 into which the cotton swab shaft is inserted is a square having a circular cross section or rounded corners. 前記中空突出部は、外周に、折り曲げ切片を嵌合できる凹部が形成されることを特徴とする、請求項1〜4のいずれかに記載の拭き取り検査用キット。 The wiping inspection kit according to any one of claims 1 to 4, wherein the hollow protrusion is formed with a recess on the outer periphery into which a bent section can be fitted. 前記蓋部は、前記切り取り切片の外周に、コンタミ防止用突起を有することを特徴とする、請求項1〜5のいずれかに記載の拭き取り検査用キット。 The wiping inspection kit according to any one of claims 1 to 5, wherein the lid portion has a protrusion for preventing contamination on the outer periphery of the cut section. 前記容器本体の底面部は、前記容器本体の外方に延設される翼が形成された平板であり、自立可能であることを特徴とする、請求項1〜6のいずれかに記載の拭き取り検査用キット。

The wiping according to any one of claims 1 to 6, wherein the bottom surface portion of the container body is a flat plate on which wings extending to the outside of the container body are formed and can stand on its own. Inspection kit.

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS584200U (en) * 1981-07-02 1983-01-11 小野薬品工業株式会社 Sample collection container set for cotton swabs
US5543115A (en) * 1995-07-17 1996-08-06 Mizuho Usa, Inc. Specimen handling device
JP2017516980A (en) * 2014-04-17 2017-06-22 セクレタリー オブ ステート フォー ヘルスSecretary Of State For Health Fluid collection device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS584200U (en) * 1981-07-02 1983-01-11 小野薬品工業株式会社 Sample collection container set for cotton swabs
US5543115A (en) * 1995-07-17 1996-08-06 Mizuho Usa, Inc. Specimen handling device
JP2017516980A (en) * 2014-04-17 2017-06-22 セクレタリー オブ ステート フォー ヘルスSecretary Of State For Health Fluid collection device

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