JP2020132578A - Antibacterial agent, molding and antibacterial method - Google Patents
Antibacterial agent, molding and antibacterial method Download PDFInfo
- Publication number
- JP2020132578A JP2020132578A JP2019028837A JP2019028837A JP2020132578A JP 2020132578 A JP2020132578 A JP 2020132578A JP 2019028837 A JP2019028837 A JP 2019028837A JP 2019028837 A JP2019028837 A JP 2019028837A JP 2020132578 A JP2020132578 A JP 2020132578A
- Authority
- JP
- Japan
- Prior art keywords
- antibacterial
- powder
- cerium
- supported
- antibacterial agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 50
- 239000003242 anti bacterial agent Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000000465 moulding Methods 0.000 title abstract 3
- 239000000843 powder Substances 0.000 claims abstract description 61
- 229910052684 Cerium Inorganic materials 0.000 claims abstract description 34
- GWXLDORMOJMVQZ-UHFFFAOYSA-N cerium Chemical compound [Ce] GWXLDORMOJMVQZ-UHFFFAOYSA-N 0.000 claims abstract description 32
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 29
- 239000000440 bentonite Substances 0.000 claims description 27
- 229910000278 bentonite Inorganic materials 0.000 claims description 27
- 210000002268 wool Anatomy 0.000 claims description 17
- 229910021536 Zeolite Inorganic materials 0.000 claims description 16
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 16
- 239000010457 zeolite Substances 0.000 claims description 16
- 239000011121 hardwood Substances 0.000 claims description 10
- -1 wool Substances 0.000 claims description 7
- 150000001768 cations Chemical class 0.000 claims description 6
- 239000000047 product Substances 0.000 description 26
- 239000000463 material Substances 0.000 description 19
- 239000004744 fabric Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 229910052901 montmorillonite Inorganic materials 0.000 description 7
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 6
- 239000002184 metal Substances 0.000 description 6
- 239000002245 particle Substances 0.000 description 5
- 239000002023 wood Substances 0.000 description 5
- HSJPMRKMPBAUAU-UHFFFAOYSA-N cerium(3+);trinitrate Chemical compound [Ce+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O HSJPMRKMPBAUAU-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 229910052709 silver Inorganic materials 0.000 description 4
- 239000004332 silver Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229910002651 NO3 Inorganic materials 0.000 description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 240000000731 Fagus sylvatica Species 0.000 description 1
- 235000010099 Fagus sylvatica Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 241000190021 Zelkova Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000004067 bulking agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 201000003373 familial cold autoinflammatory syndrome 3 Diseases 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000012802 nanoclay Substances 0.000 description 1
- 239000002114 nanocomposite Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 239000006097 ultraviolet radiation absorber Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000002759 woven fabric Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
本発明は、抗菌剤、成形体及び抗菌方法に関する。 The present invention relates to antibacterial agents, molded articles and antibacterial methods.
金属を抗菌剤に利用する技術が知られている。例えば特許文献１には、中間製品を形成させ、中間製品の少なくとも１つの構成成分を抗菌性金属コロイドで処理し、次いで抗菌性金属の易溶性塩または難溶性塩を添加する工程を含む抗菌性プラスチック製品の製造方法が開示されている。 Techniques for using metals as antibacterial agents are known. For example, Patent Document 1 includes an antibacterial property including a step of forming an intermediate product, treating at least one component of the intermediate product with an antibacterial metal colloid, and then adding an easily soluble salt or a poorly soluble salt of the antibacterial metal. A method for manufacturing a plastic product is disclosed.
また、特許文献２には、ゼオライトを担体とする抗菌性組成物及びその製造方法が開示され、例えば、天然のゼオライトに銀を保持させた抗菌性組成物の実施例が開示されている。 Further, Patent Document 2 discloses an antibacterial composition using zeolite as a carrier and a method for producing the same, and discloses, for example, an example of an antibacterial composition in which natural zeolite retains silver.
また、特許文献３には、殺菌特性等を有する、金属酸化物粒子の担体としてのナノクレイを含むナノ複合体材料が開示されている。 Further, Patent Document 3 discloses a nanocomposite material containing nanoclay as a carrier of metal oxide particles, which has bactericidal properties and the like.
また、特許文献４には、粒子状無機鉱物及び抗菌性金属を含む組成物であって、抗菌性金属が粒子状無機鉱物の粒子内に組み込まれている組成物が開示されている。 Further, Patent Document 4 discloses a composition containing a particulate inorganic mineral and an antibacterial metal, in which the antibacterial metal is incorporated in the particles of the particulate inorganic mineral.
また、非特許文献１には、セリウムを含むモンモリロナイト及びバーミキュライトが抗菌活性を有することが開示されている。 Further, Non-Patent Document 1 discloses that montmorillonite containing cerium and vermiculite have antibacterial activity.
しかしながら、特許文献１−３に記載の抗菌性材料の調製方法は、煩雑な操作を要するものであり、また、金属として銀を用いた場合には、羊毛や硫黄含有ポリマーに添加した際に硫化銀が形成され、黒色に変色するという難点を有していた。また、特許文献４及び非特許文献１に記載の抗菌性材料では、抗菌効果の点で課題を残していた。 However, the method for preparing an antibacterial material described in Patent Documents 1-3 requires complicated operations, and when silver is used as a metal, it sulfides when added to wool or a sulfur-containing polymer. It had the drawback that silver was formed and turned black. Further, the antibacterial materials described in Patent Document 4 and Non-Patent Document 1 have a problem in terms of antibacterial effect.
本発明は、上記事情に鑑みてなされたものであり、簡便に調製することができ、かつ成形体に加工しやすい抗菌剤、成形体及び抗菌方法を提供することを目的とする。 The present invention has been made in view of the above circumstances, and an object of the present invention is to provide an antibacterial agent, a molded product, and an antibacterial method that can be easily prepared and easily processed into a molded product.
上記目的を達成するため、本発明の第１の観点に係る抗菌剤は、
セリウムが担持された粉体からなる、
ことを特徴とする。
In order to achieve the above object, the antibacterial agent according to the first aspect of the present invention is
Consists of cerium-supported powder,
It is characterized by that.
例えば、前記セリウムは、３価の陽イオンである。 For example, the cerium is a trivalent cation.
例えば、前記粉体は、ゼオライト、羊毛、広葉樹及びベントナイトからなる群より少なくとも１つ選択される。 For example, the powder is selected from at least one group consisting of zeolite, wool, hardwood and bentonite.
本発明の第２の観点に係る成形体は、
本発明の第１の観点に係る抗菌剤を含む。
The molded product according to the second aspect of the present invention is
Includes an antibacterial agent according to the first aspect of the present invention.
例えば、セリウムが担持された粉体と、セリウムが担持されていない粉体と、からなる。 For example, it comprises a powder on which cerium is supported and a powder on which cerium is not supported.
本発明の第３の観点に係る抗菌方法は、
本発明の第１の観点に係る抗菌剤を添加する。
The antibacterial method according to the third aspect of the present invention is
The antibacterial agent according to the first aspect of the present invention is added.
本発明によれば、簡便に調製することができ、かつ成形体に加工しやすい抗菌剤、成形体及び抗菌方法を提供することができる。 According to the present invention, it is possible to provide an antibacterial agent, a molded product, and an antibacterial method that can be easily prepared and easily processed into a molded product.
まず、本実施形態による抗菌剤について詳細に説明する。 First, the antibacterial agent according to the present embodiment will be described in detail.
本実施形態による抗菌剤は、セリウムが担持された粉体からなることを特徴とする。 The antibacterial agent according to the present embodiment is characterized by being composed of a cerium-supported powder.
本実施形態による抗菌剤に用いられる粉体は、ゼオライト、羊毛、広葉樹及びベントナイトからなる群より少なくとも１つ選択され得る。ゼオライトとしては、例えば、硬質ゼオライトＳＵ（株式会社ＺＥＯ製）等を用いることができる。また、羊毛としては、例えば、羊毛をボールミル、ジェットミル等で粉砕して粉末状としたもの（例えば、いずみ染工製の羊毛粉末）を用いることができる。また、広葉樹としては、例えば、サクラ、ケヤキ、ブナなどの広葉樹の幹又は枝をボールミル、ジェットミル等で粉砕して粉末状としたもの（例えば、那賀ウッド製の広葉樹粉末）を用いることができる。また、ベントナイトとしては、例えば、工業用ベントナイト（ホージュンベントナイト穂高、株式会社ホージュン製）、ベンゲル（株式会社ホージュン製）、ベンゲル１１Ｋ（株式会社ホージュン製）等が例示されるが、ベントナイトの主成分であるモンモリロナイトの含有量が例えば７０％以下である天然ベントナイト（例えば、工業用ベントナイト（ホージュンベントナイト穂高、株式会社ホージュン製））が好ましい。なお、粉体を構成する粒子の平均粒径は、例えば、５〜１８０μｍである。 The powder used for the antibacterial agent according to the present embodiment may be selected from at least one group consisting of zeolite, wool, hardwood and bentonite. As the zeolite, for example, hard zeolite SU (manufactured by ZEO Co., Ltd.) or the like can be used. Further, as the wool, for example, wool powder obtained by crushing wool with a ball mill, jet mill or the like to form a powder (for example, wool powder manufactured by Izumi Dyeing Co., Ltd.) can be used. As the hardwood, for example, a hardwood trunk or branch of a hardwood such as cherry, zelkova, or beech may be crushed with a ball mill, a jet mill, or the like into a powder (for example, a hardwood powder made by Naga Wood). .. Examples of bentonite include industrial bentonite (Hojun bentonite Hotaka, manufactured by Hojun Co., Ltd.), Bengel (manufactured by Hojun Co., Ltd.), Bengel 11K (manufactured by Hojun Co., Ltd.), and the like, which are the main components of bentonite. Natural bentonite having a certain montmorillonite content of, for example, 70% or less (for example, industrial bentonite (for example, Hojun bentonite Hotaka, manufactured by Hojun Co., Ltd.)) is preferable. The average particle size of the particles constituting the powder is, for example, 5 to 180 μm.
粉体に担持されたセリウムは、好ましくは、３価の陽イオンである。粉体へのセリウム（Ｃｅ）の担持量は、例えば、０．３〜５．０ｍｇ−Ｃｅ／ｇ−粉体（材料）である。本明細書において「担持」とは、特定の理論に縛られることを望むものではないが、粉体中に含まれる陽イオンと３価のセリウムイオンとが交換されることで粉体にセリウムが結合している状態、粉体中に含まれる陰イオンと３価のセリウムイオンがイオン結合している状態等を指す。 The cerium supported on the powder is preferably a trivalent cation. The amount of cerium (Ce) supported on the powder is, for example, 0.3 to 5.0 mg-Ce / g-powder (material). In the present specification, "supporting" is not intended to be bound by a specific theory, but cerium is added to the powder by exchanging cations contained in the powder with trivalent cerium ions. It refers to a state in which the anion contained in the powder and a trivalent cerium ion are ionically bonded.
本実施形態による抗菌剤の抗菌活性は、これに限定されるものではないが、例えば、ＪＩＳ Ｚ ２８０１に準拠して、混釈平板培養法にて、試験菌として黄色ぶどう球菌（例えば、Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ ＮＢＲＣ１２７３２）を用いて評価することができる。この評価方法では、抗菌活性値が２以上であれば、十分な抗菌活性を示すことが知られている。なお、本実施形態による抗菌剤は、抗菌効果の他、抗菌効果に基づく防腐効果、制菌効果、防臭効果等をも有する。 The antibacterial activity of the antibacterial agent according to the present embodiment is not limited to this, but for example, in accordance with JIS Z 2801, Staphylococcus aureus (for example, Staphylococcus aureus) as a test bacterium in a pour plate culture method. It can be evaluated using NBRC12732). In this evaluation method, it is known that when the antibacterial activity value is 2 or more, sufficient antibacterial activity is exhibited. The antibacterial agent according to the present embodiment has an antiseptic effect, an antibacterial effect, a deodorant effect, etc. based on the antibacterial effect in addition to the antibacterial effect.
本実施形態による抗菌剤の調製方法の一例について説明する。ベントナイト（粉体）を、セリウム（Ｃｅ）硝酸塩水溶液（４００ｐｐｍ前後の所定の濃度）の中に浸漬し、大気圧下、温度２５℃で２４時間処理する。ここで、ベントナイトとセリウム硝酸塩水溶液との量比は、１０ｇ−材料／Ｌ−Ｃｅ水溶液とする。その後、水洗し風乾等の乾燥をする。このように、本実施形態による抗菌剤は、簡便な操作により調製することができる。 An example of the method for preparing the antibacterial agent according to the present embodiment will be described. Bentonite (powder) is immersed in a cerium (Ce) nitrate aqueous solution (predetermined concentration of around 400 ppm) and treated at atmospheric pressure at a temperature of 25 ° C. for 24 hours. Here, the amount ratio of bentonite to the cerium nitrate aqueous solution is 10 g-material / L-Ce aqueous solution. After that, it is washed with water and dried by air drying. As described above, the antibacterial agent according to the present embodiment can be prepared by a simple operation.
次に、本実施形態による成形体について説明する。 Next, the molded product according to the present embodiment will be described.
本実施形態による成形体は、前述の抗菌剤を含む。成形体は、プラスチック、合成木材等であり、本実施形態による抗菌剤を含有させることにより、抗菌活性を有する成形体（プラスチック、合成木材等）を作製することができる。本実施形態による抗菌剤は、銀を含まないため、成形体に添加した場合でも変色することがなく、加工しやすいという点でメリットを有している。 The molded product according to the present embodiment contains the above-mentioned antibacterial agent. The molded product is plastic, synthetic wood, or the like, and by incorporating the antibacterial agent according to the present embodiment, a molded product (plastic, synthetic wood, etc.) having antibacterial activity can be produced. Since the antibacterial agent according to the present embodiment does not contain silver, it does not discolor even when added to a molded product, and has an advantage in that it is easy to process.
また、本実施形態による成形体は、例えば、セリウムが担持された粉体と、セリウムが担持されていない粉体と、からなる。すなわち、該成形体に、例えば、粉体として羊毛が用いられる場合、成形体は主原料の粉体としては羊毛のみから構成され、他の種類の粉体は含まず、羊毛の一部又は全部においてセリウムが担持されている。ここで、主原料の粉体には、例えば紫外線吸収剤や安定化剤、あるいは主に無機物である増量剤等が含まれない。このように、セリウムが担持された粉体と同一種類の粉体とを混合させて成形体としてもよく、この場合、成形体が同一種類の粉体からなるため、該粉体の特性を材料設計通りに十分に発揮させることができる（機械的強度及び弾性の維持等）。なお、成形体を構成する粉体の一部にセリウムを担持させて抗菌性を付与する場合、同一種類の粉体を用いることで、粉体の一部にセリウムを担持させて抗菌性を付与し、その後、セリウムを担持させていない粉体と混合することで成形（樹脂化等）することができる。成形体を構成する粉体の一部にセリウムを担持させる場合、粉体の全部にセリウムを担持させる場合に比べて、少量の粉体の処理で済むため、セリウムの担持のための設備をコンパクトにすることができ、設備費の低減を図ることができる。 Further, the molded product according to the present embodiment is composed of, for example, a powder in which cerium is supported and a powder in which cerium is not supported. That is, when wool is used as the powder in the molded body, for example, the molded body is composed of only wool as the powder of the main raw material, does not contain other types of powder, and part or all of the wool. Cerium is carried in. Here, the powder of the main raw material does not include, for example, an ultraviolet absorber, a stabilizer, or a bulking agent which is mainly an inorganic substance. In this way, the powder on which cerium is carried and the powder of the same type may be mixed to form a molded product. In this case, since the molded body is made of the same type of powder, the characteristics of the powder are used as a material. It can be fully exerted as designed (maintenance of mechanical strength and elasticity, etc.). When cerium is supported on a part of the powder constituting the molded product to impart antibacterial properties, by using the same type of powder, cerium is supported on a part of the powder to impart antibacterial properties. After that, it can be molded (resinized, etc.) by mixing it with a powder that does not carry cerium. When cerium is supported on a part of the powder constituting the molded body, a small amount of powder can be processed as compared with the case where cerium is supported on the entire powder, so that the equipment for supporting cerium is compact. It is possible to reduce the equipment cost.
なお、セリウムが担持された粉体と異なる種類の粉体とを混合させて成形体としてもよい。また、セリウムが担持された粉体に、樹脂材料（例えば、Ｐｏｌｙｍｅｔｈｙｌ ｍｅｔｈａｃｒｙｌａｔｅ）、合成木材等を混合させて成形体としてもよい。 In addition, a powder in which cerium is supported and a powder of a different type may be mixed to form a molded product. Further, a resin material (for example, Polymethyl methyllate), synthetic wood, or the like may be mixed with the cerium-supported powder to form a molded product.
本実施形態による成形体は、前述の抗菌剤を含み、該抗菌剤はセリウムが担持された粉体からなる。セリウムは、例えば、３価の陽イオンである。該粉体は、例えば、ゼオライト、羊毛、広葉樹及びベントナイトからなる群より少なくとも１つ選択される。これらの詳細については、前述同様である。 The molded product according to the present embodiment contains the above-mentioned antibacterial agent, and the antibacterial agent consists of a cerium-supported powder. Cerium is, for example, a trivalent cation. The powder is selected from, for example, at least one from the group consisting of zeolite, wool, hardwood and bentonite. The details thereof are the same as described above.
本実施形態による成形体の作製方法の一例について説明する。Ｐｏｌｙｍｅｔｈｙｌ ｍｅｔｈａｃｒｙｌａｔｅ（ＰＭＭＡ）と、本実施形態による抗菌剤（例えば、セリウムを担持させたベントナイト）と、を質量比９０：１０の割合で配合し、乳鉢で５分間混合したものを温度１４０℃、圧力６０ＭＰａで加熱・加圧処理して成形体を得る。 An example of a method for producing a molded product according to the present embodiment will be described. Polymethyl methacrylicate (PMMA) and an antibacterial agent according to the present embodiment (for example, bentonite carrying cerium) are mixed at a mass ratio of 90:10 and mixed in a dairy pot for 5 minutes at a temperature of 140 ° C. and a pressure. A molded product is obtained by heating and pressurizing at 60 MPa.
次に、本実施形態による抗菌方法について説明する。 Next, the antibacterial method according to this embodiment will be described.
本実施形態による抗菌方法は、前述の抗菌剤を添加することを特徴とする。該抗菌剤はセリウムが担持された粉体からなる。セリウムは、例えば、３価の陽イオンである。該粉体は、例えば、ゼオライト、羊毛、広葉樹及びベントナイトからなる群より少なくとも１つ選択される。これらの詳細については、前述同様である。 The antibacterial method according to the present embodiment is characterized by adding the above-mentioned antibacterial agent. The antibacterial agent consists of a cerium-supported powder. Cerium is, for example, a trivalent cation. The powder is selected from, for example, at least one from the group consisting of zeolite, wool, hardwood and bentonite. The details thereof are the same as described above.
以上説明したように、本実施形態による抗菌剤は、煩雑な操作を要することなく簡便かつ低コストで調製することができる。また、成形体に添加した場合でも変色等を生じることなく、成形体に加工しやすいという利点を有している。このように、易加工性を有しつつ簡便に調製できる本実施形態による抗菌剤は、優れた抗菌活性を有する材料として、成形体の加工等において幅広く利用されることが期待される。 As described above, the antibacterial agent according to the present embodiment can be prepared easily and at low cost without requiring complicated operations. Further, it has an advantage that it can be easily processed into a molded product without causing discoloration even when it is added to the molded product. As described above, the antibacterial agent according to the present embodiment, which has easy processability and can be easily prepared, is expected to be widely used in the processing of molded products as a material having excellent antibacterial activity.
以下、実施例を挙げて本発明を具体的に説明する。ただし、本発明はこれらの実施例に限定されるものではない。 Hereinafter, the present invention will be specifically described with reference to examples. However, the present invention is not limited to these examples.
（実施例１）
（セリウムを担持させた各種粉体材料の抗菌性評価）
以下の粉体材料にセリウム（Ｃｅ）を担持させ、抗菌性を評価した。なお、比較例として、羊毛（織布）及びシルク（織布）を用いた。本実施例の操作手順を図１に示す。
・ゼオライト（株式会社ＺＥＯ製、硬質ゼオライトＳＵ ８０メッシュアンダー）
・羊毛（粉末）（いずみ染工製、平均粒径６μｍ）
・広葉樹（那賀ウッド製）
・工業用ベントナイト（ホージュンベントナイト穂高、株式会社ホージュン製）：モンモリロナイト含有量６０〜７０％（ベントナイト鉱石を原料とする）
・ベンゲル（株式会社ホージュン製）：モンモリロナイト含有量８０〜９０％（ベントナイト鉱石を原料とする）
・ベンゲル１１Ｋ（株式会社ホージュン製）：モンモリロナイト含有量８０〜９０％（ベントナイト鉱石から特殊な分級精製処理プロセスを経て得られたＣａ型ホワイトベントナイトで、白色度が極めて高く、鉄分含有量が少ないことが特徴である）
(Example 1)
(Evaluation of antibacterial properties of various powder materials carrying cerium)
Cerium (Ce) was supported on the following powder materials, and the antibacterial properties were evaluated. As a comparative example, wool (woven cloth) and silk (woven cloth) were used. The operation procedure of this embodiment is shown in FIG.
・ Zeolite (manufactured by ZEO Co., Ltd., hard zeolite SU 80 mesh under)
・ Wool (powder) (manufactured by Izumi Dyeing Co., Ltd., average particle size 6 μm)
・ Hardwood (made by Naga Wood)
-Industrial bentonite (Hojun bentonite Hotaka, manufactured by Hojun Co., Ltd.): Montmorillonite content 60-70% (using bentonite ore as a raw material)
-Wenger (manufactured by Hojun Co., Ltd.): Montmorillonite content 80-90% (using bentonite ore as a raw material)
Wenger 11K (manufactured by Hojun Co., Ltd.): Montmorillonite content 80-90% (Ca-type white bentonite obtained from bentonite ore through a special classification and refining process, with extremely high whiteness and low iron content. Is a feature)
上記の各粉体材料を、セリウム（Ｃｅ）硝酸塩水溶液（４００ｐｐｍ前後の所定の濃度）の中に浸漬し、大気圧下、温度２５℃で２４時間担持処理した。ここで、各種粉体材料とセリウム硝酸塩水溶液との量比は、１０ｇ−材料／Ｌ−Ｃｅ水溶液とした。その後、水洗し風乾した。比較例の羊毛（織布）及びシルク（織布）においては、織布の状態で硝酸塩水溶液（４００ｐｐｍ前後の所定の濃度）の中に浸漬し、大気圧下、温度２５℃で２４時間担持処理した。 Each of the above powder materials was immersed in an aqueous solution of cerium (Ce) nitrate (a predetermined concentration of about 400 ppm) and supported on a surface at a temperature of 25 ° C. for 24 hours under atmospheric pressure. Here, the amount ratio of various powder materials to the cerium nitrate aqueous solution was 10 g-material / L-Ce aqueous solution. After that, it was washed with water and air-dried. In the wool (woven cloth) and silk (woven cloth) of the comparative example, the wool (woven cloth) and silk (woven cloth) are immersed in a nitrate aqueous solution (predetermined concentration of about 400 ppm) in the state of the woven cloth, and supported at a temperature of 25 ° C. for 24 hours under atmospheric pressure. did.
各種材料に対するＣｅ担持量を表１に示す。実施例では粉末状態での担持量であり、比較例では織布状態での担持量を表す。実施例の各種粉体材料では、相当量のＣｅが担持していることが示され、工業用ベントナイトでは、特にＣｅ担持量が高いことがわかった。すなわち、モンモリロナイトの含有量が低いほど、Ｃｅ担持量が高いことが示された。また、Ｃｅが担持された各種粉体材料は、後述する抗菌活性を発揮することが示された。 Table 1 shows the amount of Ce carried on various materials. In the examples, it represents the amount supported in the powder state, and in the comparative example, it represents the amount supported in the woven fabric state. It was shown that the various powder materials of the examples supported a considerable amount of Ce, and that the industrial bentonite supported a particularly high amount of Ce. That is, it was shown that the lower the content of montmorillonite, the higher the amount of Ce carried. Further, it was shown that various powder materials on which Ce is supported exhibit antibacterial activity described later.
各種粉体の抗菌試験はＪＩＳ Ｚ ２８０１に準拠して行った。各種粉体は、精製水９ｍＬに対して、１０％の濃度となるよう調製された。この試験の実施を、一般社団法人ニッセンケン品質評価センターに依頼した。
・試験方法
（１）保存菌を平板培地上に分画し、２４時間培養した。
（２）培養後の平板培地よりコロニーを１白金耳とり、新たな平板培地上に植菌し、３７℃で２４時間培養した。
（３）培養後の平板培地よりコロニーを１白金耳とり、１／５００ＮＢに懸濁した。
（４）約１０５ＣＦＵ／ｍＬとなるように１／５００ＮＢ培地で調製し、これを試験菌液とした。
（５）試験試料及び対照試料（ＰＢＳ）９ｍＬに試験菌液を１ｍＬ添加し、３７℃で２４時間振盪培養を行った。
（６）２４時間後、１ｍＬを取り出し、９ｍＬのＳＣＤＬＰ培地と混合し反応を停止した。
（７）その内の１ｍＬを取り出し、９ｍＬの精製水に混合し、順次１０倍希釈系列を作製した。
（８）それぞれの希釈系列より１ｍＬをシャーレに採り、寒天培地を加えた。
（９）寒天が固化したら、３７℃で４８時間培養した。
（１０）４８時間後コロニー数を計数し、生菌数を求めた。
（参考規格：ＪＩＳ Ｚ ２８０１：２０１０）
・測定方法：混釈平板培養法
・試験菌：黄色ぶどう球菌 Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ ＮＢＲＣ１２７３２
Antibacterial tests of various powders were carried out in accordance with JIS Z 2801. The various powders were prepared to have a concentration of 10% with respect to 9 mL of purified water. We requested the Nissenken Quality Evaluation Center to carry out this test.
-Test method (1) The preserved bacteria were fractionated on a plate medium and cultured for 24 hours.
(2) One platinum loop was taken from the plate medium after culturing, inoculated on a new plate medium, and cultured at 37 ° C. for 24 hours.
(3) One platinum loop was taken from the plate medium after culturing and suspended at 1/500 NB.
(4) to be about 10 5 CFU / mL were prepared in 1 / 500NB medium, which was used as a test bacterial solution.
(5) 1 mL of the test bacterial solution was added to 9 mL of the test sample and the control sample (PBS), and the mixture was cultured with shaking at 37 ° C. for 24 hours.
(6) After 24 hours, 1 mL was taken out and mixed with 9 mL of SCDLP medium to stop the reaction.
(7) 1 mL of it was taken out and mixed with 9 mL of purified water to prepare a 10-fold dilution series in sequence.
(8) 1 mL of each dilution series was taken into a petri dish, and an agar medium was added.
(9) When the agar had solidified, it was cultured at 37 ° C. for 48 hours.
(10) After 48 hours, the number of colonies was counted to determine the number of viable bacteria.
(Reference standard: JIS Z 2801: 2010)
-Measurement method: Plaid plate culture method-Test bacteria: Staphylococcus aureus NBRC12732
また、比較例（参考例）の抗菌試験を以下の通り行った。ＪＩＳ Ｌ１９０２による菌液吸収法において、接触直後の生菌数の常用対数値と、１８時間培養後の生菌数の常用対数値と、で比較した。この試験の実施を、一般社団法人カケンテストセンターに依頼した。抗菌活性値は、「標準布の１８時間後の菌数の常用対数値」−「抗菌加工布の１８時間後の菌数の常用対数値」で算出した。また、Ｆ値は、「標準布の１８時間後の菌数の常用対数値」−「抗菌加工布の１８時間後の菌数の常用対数値」で算出した。 In addition, an antibacterial test of a comparative example (reference example) was conducted as follows. In the bacterial solution absorption method according to JIS L1902, the common logarithmic value of the viable cell count immediately after contact and the common logarithmic value of the viable cell count after culturing for 18 hours were compared. We requested the Kaken Test Center, a general incorporated association, to carry out this test. The antibacterial activity value was calculated by "the common logarithmic value of the number of bacteria 18 hours after the standard cloth"-"the common logarithmic value of the number of bacteria 18 hours after the antibacterial processed cloth". The F value was calculated by "the common logarithmic value of the number of bacteria 18 hours after the standard cloth"-"the common logarithmic value of the number of bacteria 18 hours after the antibacterial processed cloth".
抗菌活性値を表１に示す。実施例の各種粉体材料では、抗菌活性値が２以上となっており、抗菌効果を有することが示された。 The antibacterial activity values are shown in Table 1. The various powder materials of the examples had an antibacterial activity value of 2 or more, indicating that they have an antibacterial effect.
（実施例２）
（セリウムを担持させた粉体材料のＰＭＭＡ混合時の抗菌性評価）
実施例１で得られた、セリウムを担持させたゼオライト及び工業用ベントナイトを、Ｐｏｌｙｍｅｔｈｙｌ ｍｅｔｈａｃｒｙｌａｔｅ（ＰＭＭＡ）に添加し、抗菌性を評価した。なお、比較例として、何も添加していないＰＭＭＡ並びにセリウム非担持のゼオライト及び工業用ベントナイトを用いた。本実施例の操作手順を図２に示す。
(Example 2)
(Evaluation of antibacterial properties when PMMA is mixed with powder material carrying cerium)
The cerium-supported zeolite and industrial bentonite obtained in Example 1 were added to Polymethyl methylate (PMMA), and the antibacterial properties were evaluated. As comparative examples, PMMA to which nothing was added, zeolite not supporting cerium, and industrial bentonite were used. The operation procedure of this embodiment is shown in FIG.
ＰＭＭＡ（品番：４４５７４６−５００Ｇ、ロット番号：ＭＫＢＨ８７３２、アルドリッチ社製、分子量：Ｍ＜３５０，０００）と、実施例１で得られたセリウムを担持させたゼオライト又はセリウムを担持させた工業用ベントナイトと、を質量比９０：１０の割合で配合し、乳鉢で５分間混合したものを温度１４０℃、圧力６０ＭＰａで加熱・加圧処理して成形体を得た。比較例の何も添加していないＰＭＭＡ並びにセリウム非担持のゼオライト及び工業用ベントナイトにおいても同様にＰＭＭＡに配合し、同様に成形体を得た。得られた成形体について、抗菌試験を実施例１と同様に行った。 PMMA (product number: 445746-500G, lot number: MKBH8732, manufactured by Aldrich, molecular weight: M <350,000), and the cerium-supported zeolite obtained in Example 1 or cerium-supported industrial bentonite. , Was blended at a mass ratio of 90:10, mixed in a dairy pot for 5 minutes, and heated and pressurized at a temperature of 140 ° C. and a pressure of 60 MPa to obtain a molded product. PMMA to which nothing was added in the comparative example, cerium-free zeolite, and industrial bentonite were also blended with PMMA in the same manner to obtain a molded product in the same manner. An antibacterial test was carried out on the obtained molded product in the same manner as in Example 1.
結果を表２に示す。実施例の各種粉体材料では、抗菌活性値が２以上となっており、抗菌効果を有することが示された。一方で、何も添加していないＰＭＭＡ並びにセリウム非担持のゼオライト及び工業用ベントナイトを用いた比較例では、抗菌活性値は２未満であり、抗菌効果を有しないことが示された。 The results are shown in Table 2. The various powder materials of the examples had an antibacterial activity value of 2 or more, indicating that they have an antibacterial effect. On the other hand, in a comparative example using PMMA to which nothing was added, zeolite non-supporting cerium, and industrial bentonite, the antibacterial activity value was less than 2, indicating that it had no antibacterial effect.
Claims (6)
ことを特徴とする抗菌剤。 Consists of cerium-supported powder,
An antibacterial agent characterized by that.
ことを特徴とする請求項１に記載の抗菌剤。 The cerium is a trivalent cation,
The antibacterial agent according to claim 1.
ことを特徴とする請求項１又は２に記載の抗菌剤。 The powder is selected from at least one group consisting of zeolite, wool, hardwood and bentonite.
The antibacterial agent according to claim 1 or 2.
ことを特徴とする請求項４に記載の成形体。 It consists of a powder on which cerium is supported and a powder on which cerium is not supported.
The molded product according to claim 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019028837A JP7329815B2 (en) | 2019-02-20 | 2019-02-20 | Antibacterial agent, molded article, antibacterial method, and method for producing antibacterial agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019028837A JP7329815B2 (en) | 2019-02-20 | 2019-02-20 | Antibacterial agent, molded article, antibacterial method, and method for producing antibacterial agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020132578A true JP2020132578A (en) | 2020-08-31 |
| JP7329815B2 JP7329815B2 (en) | 2023-08-21 |
Family
ID=72277655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019028837A Active JP7329815B2 (en) | 2019-02-20 | 2019-02-20 | Antibacterial agent, molded article, antibacterial method, and method for producing antibacterial agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP7329815B2 (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03255009A (en) * | 1990-03-05 | 1991-11-13 | Kanebo Ltd | Antibacterial zeolite and its production |
| JPH08301713A (en) * | 1995-03-07 | 1996-11-19 | Fumakilla Ltd | Termite expellent and extermination of termite using the same |
| JPH1157223A (en) * | 1997-08-27 | 1999-03-02 | Misawa Homes Co Ltd | Playground equipment |
| WO2009028234A1 (en) * | 2007-08-28 | 2009-03-05 | Pinole Co., Ltd. | Hair increasing dye and aerosol type hair increasing die |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3255009B2 (en) | 1996-04-30 | 2002-02-12 | 安藤電気株式会社 | Optical spectrum measurement device |
-
2019
- 2019-02-20 JP JP2019028837A patent/JP7329815B2/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03255009A (en) * | 1990-03-05 | 1991-11-13 | Kanebo Ltd | Antibacterial zeolite and its production |
| JPH08301713A (en) * | 1995-03-07 | 1996-11-19 | Fumakilla Ltd | Termite expellent and extermination of termite using the same |
| JPH1157223A (en) * | 1997-08-27 | 1999-03-02 | Misawa Homes Co Ltd | Playground equipment |
| WO2009028234A1 (en) * | 2007-08-28 | 2009-03-05 | Pinole Co., Ltd. | Hair increasing dye and aerosol type hair increasing die |
Non-Patent Citations (2)
| Title |
|---|
| HUNDAKOVA, MARIANNA ET AL.: "Antibacterial Cerium-Montmorillonite and Cerium-Vermiculite", CONFERENCE PROCEEDINGS - NANOCON 2014, JPN7022005167, 2015, pages 633 - 638, ISSN: 0005026554 * |
| JOURNAL OF BACTERIOLOGY, vol. 54, no. 4, JPN6022046504, 1947, pages 417 - 424, ISSN: 0005026555 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP7329815B2 (en) | 2023-08-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ni et al. | Synthesis of silver nanoparticle-decorated hydroxyapatite (HA@ Ag) poriferous nanocomposites and the study of their antibacterial activities | |
| CN108589266B (en) | Preparation method of nano metal particle/metal organic framework composite antibacterial cellulose fiber | |
| Shariatinia et al. | Mechanical properties and antibacterial activities of novel nanobiocomposite films of chitosan and starch | |
| Guibal et al. | Silver/chitosan/cellulose fibers foam composites: from synthesis to antibacterial properties | |
| Yuvaraja et al. | Preparation of novel aminated chitosan schiff’s base derivative for the removal of methyl orange dye from aqueous environment and its biological applications | |
| KR101794243B1 (en) | Composition for adsorbing and decomposing a compound inducing an odor, product comprising the same and method for preparing the same | |
| JPS60181002A (en) | Antimicrobial composition containing zeolite as carrier and production thereof | |
| CN110373899B (en) | Washable antibacterial cotton fabric and preparation method thereof | |
| Pourabolghasem et al. | Antibacterial Activity of Copper-doped Montmorillonite Nanocomposites Prepared by Alkaline Ion Exchange Method. | |
| CN112442893A (en) | Special efficient nano copper antibacterial agent and preparation method thereof | |
| CN100551242C (en) | Silver supported ammonium modified nano zeolite antibacterial agent and preparation method thereof | |
| JPS62241932A (en) | Polymer foam having antibacterial and antifungal function and its production | |
| CN110527756B (en) | Environment-friendly efficient nano antibacterial agent for leather and preparation method and application thereof | |
| Rathnayake et al. | Imparting antimicrobial properties to natural rubber latex foam via green synthesized silver nanoparticles | |
| Rocha Oliveira et al. | Bactericidal performance of chlorophyllin-copper hydrotalcite compounds | |
| JP2020132578A (en) | Antibacterial agent, molding and antibacterial method | |
| Sayehi et al. | Using a zeolite produced from glass waste and aluminum scraps to develop a novel gelatin-based biodegradable composites films: antibacterial and antioxidant properties of a potential food packaging material | |
| Chen et al. | Preparation of grafted cationic polymer/silver chloride modified cellulose fibers and their antibacterial properties | |
| KR101981894B1 (en) | Antibacterial fibers used in kendo goods and so on | |
| CN112831068B (en) | Preparation method of novel antibacterial composite material | |
| Ohashi et al. | Antimicrobial activity and thermostability of silver 6-benzylaminopurine montmorillonite | |
| Ma et al. | Antibacterial activity and mechanism of ZnO/Cu2+-chitosan/montmorillonite | |
| JP2005510578A (en) | Antibacterial composite material | |
| KR101709220B1 (en) | Enhanced moldability a antimicrobial plastic | |
| Cardoso et al. | Antimicrobial materials properties based on ion-exchange 4A zeolite derivatives |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20211223 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20221012 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20221101 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20221227 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230404 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230526 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20230725 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20230801 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 7329815 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |