JP2020075887A - Biofilm removers and methods for removing biofilms - Google Patents

Abstract

To provide biofilm removers excellent in biofilm removing performance and low in loads to human bodies.SOLUTION: The biofilm remover of the invention comprises water and fatty acid potassium, where the fatty acid potassium is at least one selected from the group consisting of: (A) potassium oleate; and (B) potassium laurate and potassium caprate, and the content of the fatty acid potassium is 6 mass% or more and 34 mass% or less of the biofilm remover, the pure content of the ingredient (A) to the total of pure content of (A) ingredient and pure content of (B) ingredient is 6 mass% or more and 85 mass% or less, and the total of the pure content of (A) ingredient and the pure content of (B) ingredient is 75 mass% or more of the total amount of the fatty acid potassium.SELECTED DRAWING: None

Description

  The present invention relates to a biofilm removing agent and a biofilm removing method.

  A biofilm (pellicle) is a structure formed by microorganisms. When a biofilm is formed, there is a possibility that harm caused by microorganisms may occur and various effects on the human body. Further, the effect of a microbial control agent such as a bactericide tends not to be sufficiently exerted on the biofilm-formed microbial aggregate, as compared with the case of a microorganism in a suspended state in a water system.

Then, the technique regarding the skin cleanser composition etc. which can remove this biofilm is proposed (for example, refer patent documents 1-3).
However, the skin cleanser composition described in Patent Document 1 and the biofilm remover described in Patent Document 3 have a problem that they have relatively high irritation to the skin and a large load on the human body. .. On the other hand, the skin cleansing composition described in Patent Document 2 is not always sufficient in terms of biofilm removal performance.

JP, 2010-241740, A JP, 2013-124229, A JP, 2013-185036, A

  An object of the present invention is to provide a biofilm removing agent having excellent biofilm removing performance and less burden on the human body, and a biofilm removing method using the same.

In order to solve the above problems, the present invention provides the following biofilm removing agent and biofilm removing method.
That is, the biofilm remover of the present invention is a biofilm remover comprising water and potassium fatty acid, wherein the fatty acid potassium is (A) potassium oleate, (B) potassium laurate and potassium caprate. At least one selected from the group consisting of, and the content of the fatty acid potassium is 6% by mass or more and 34% by mass or less based on the total amount of the biofilm removing agent, and the component (A). Content of 6% by mass or more and 85% by mass or less, based on the total amount of the pure components (A) and (B), and the components (A) and (B). The total amount of pure components is 75% by mass or more with respect to the total amount of potassium fatty acid.

  The biofilm removing agent of the present invention preferably contains no organic solvent.

  The biofilm removal method of the present invention is a biofilm removal method using the biofilm removal agent, the step of applying the biofilm removal agent to an object to be treated, and the biofilm removal agent applied And a step of removing the biofilm by washing the treated material.

  ADVANTAGE OF THE INVENTION According to this invention, the biofilm removal performance is excellent, and the biofilm removal agent with few loads to a human body, and the biofilm removal method using the same can be provided.

Hereinafter, embodiments of the biofilm removing agent and the biofilm removing method of the present invention will be described.
[Biofilm remover]
First, the biofilm removing agent of this embodiment will be described.
The biofilm removing agent of the present embodiment is composed of water and potassium fatty acid described below.
The biofilm removing agent of the present embodiment preferably consists of water and potassium fatty acid alone. The biofilm removal performance can be secured only by this fatty acid potassium. And in the biofilm removal agent of this embodiment, it is not necessary to use a biofilm removal component other than potassium fatty acid.
In addition, in the biofilm removing agent of the present embodiment, from the viewpoint of not using unnecessary components, it is preferable not to include components other than potassium fatty acid (for example, organic solvent (alcohol, etc.), antibacterial agent, etc.). ..

The biofilm removing agent of the present embodiment is composed of water and potassium fatty acid alone, and preferably does not substantially contain components other than these components.
The water used for the biofilm removing agent of the present embodiment is preferably purified water. Examples of the purified water include RO (Reverse Osmosis) water, deionized water, and distilled water. Further, the purified water is more preferably purified water that conforms to the Japanese Pharmacopoeia or quasi-drug raw material specification.

  The fatty acid potassium used in the present embodiment needs to contain (A) potassium oleate and (B) at least one selected from the group consisting of potassium laurate and potassium caprate. By combining the component (A) and the component (B), the stability of the liquid as a biofilm removing agent can be improved, and foaming and foam retention can be improved.

The component (A) used in the present embodiment is a potassium salt of oleic acid, which is a fatty acid that is naturally present in a large amount, and it has been confirmed that the load on the human body is small.
The potassium oleate used in this embodiment is obtained from oleic acid as a raw material and a potassium compound (potassium hydroxide or the like). Here, oleic acid as a raw material usually contains impurities. Most of the impurities are linoleic acid, but other than that, stearic acid, linolenic acid, palmitic acid and the like are contained.

The component (B) used in this embodiment is a potassium salt of lauric acid or capric acid, which are fatty acids that are naturally present in a large amount, and it has been confirmed that the load on the human body is small.
The potassium laurate used in this embodiment is obtained from lauric acid as a raw material and a potassium compound (potassium hydroxide or the like). Here, lauric acid as a raw material usually contains a small amount of impurities. The impurities include capric acid and myristic acid.
Potassium caprate (potassium decanoate) used in this embodiment is obtained from capric acid as a raw material and a potassium compound (potassium hydroxide or the like). Here, capric acid as a raw material usually contains a small amount of impurities. The impurities include caprylic acid and lauric acid.

  Impurities other than the components (A) and (B) include potassium caprylate, potassium myristate, potassium palmitate, potassium stearate, potassium palmitooleate, potassium linoleate, potassium linolenate, potassium arachidate and behen. Examples thereof include potassium acid.

In the present embodiment, the content of potassium fatty acid needs to be 6% by mass or more and 34% by mass or less based on the total amount of the biofilm removing agent. When the content of potassium fatty acid is less than 6% by mass, the biofilm removing agent has insufficient foaming and foam retention. On the other hand, when the content of fatty acid potassium exceeds 34% by mass, the biofilm removing agent becomes gel.
The content of the fatty acid potassium is preferably 7% by mass or more, more preferably 10% by mass or more, and more preferably 15% by mass with respect to the total amount of the biofilm removing agent from the viewpoint that foaming and foam retention can be improved. % Or more is particularly preferable.
The content of potassium fatty acid is preferably 30% by mass or less, and more preferably 25% by mass or less, based on the total amount of the biofilm removing agent, from the viewpoint of improving the liquid stability.

In this embodiment, the content of the pure component (A) must be 6% by mass or more and 85% by mass or less based on the total amount of the pure components (A) and (B). Is. If the content of the pure component (A) is less than 6% by mass, the biofilm removing agent will have insufficient foaming and foam retention. On the other hand, when the content of the pure component (A) exceeds 85% by mass, the stability of the liquid is deteriorated, and the biofilm removing agent has insufficient foaming and foam retention.
The content of the pure component (A) is 10% by mass or more with respect to the total amount of the pure components (A) and (B) from the viewpoint that foaming and foam retention can be improved. It is more preferably 15% by mass or more.
From the viewpoint that the liquid content stability can be improved, and foaming and foam retention can be improved, the content of the pure component (A) is based on the total amount of the pure components (A) and (B). It is preferably 70% by mass or less, more preferably 60% by mass or less, and particularly preferably 50% by mass or less.

In the present embodiment, it is necessary that the total amount of the pure components (A) and (B) is 75% by mass or more based on the total amount of potassium fatty acid. When the total amount of the pure components (A) and (B) is less than 75% by mass, the liquid stability of the biofilm removing agent becomes insufficient.
The total amount of the pure components (A) and (B) is preferably 80% by mass or more and 85% by mass or more based on the total amount of potassium fatty acid from the viewpoint of improving the stability of the liquid. It is more preferable to be present, and particularly preferably 90% by mass or more.
The total amount of the pure components (A) and (B) is preferably 98% by mass or less and 95% by mass with respect to the total amount of fatty acid potassium from the viewpoint of ease of production from natural materials. The content is more preferably below, and particularly preferably 92 mass% or below.

The biofilm removing agent of the present embodiment may contain an additive as long as the object of the present invention is not impaired. Examples of the additive include at least one selected from the group consisting of a pH adjuster and a chelating agent. Further, more specific additives are selected from the group consisting of citric acid, tartaric acid, lactic acid, malic acid, oxalic acid, malonic acid, fumaric acid, succinic acid, glycolic acid, phytic acid, or salts thereof. At least 1 type is mentioned.
Moreover, when the above-mentioned additive is used, the biofilm removing agent of the present embodiment preferably comprises water, potassium fatty acid, and the above-mentioned additive.

  The biofilm removing agent of the present embodiment can be produced by mixing water and potassium fatty acid in the above-mentioned predetermined ratios and stirring and mixing.

[Biofilm removal method]
Next, the biofilm removal method of this embodiment will be described.
The biofilm removal method of the present embodiment is a biofilm removal method using the biofilm removal agent of the present embodiment, which includes a coating step and a removal step described below.

In the coating step, the biofilm removing agent is coated on the object to be treated.
Here, the biofilm removing agent is preferably foamy. As a method for converting a liquid biofilm remover into a foamy biofilm remover, a conventionally known method using a whisk can be adopted. The biofilm remover may be applied on the object to be treated and then foamed.
Examples of the object to be processed include a human body (hand, arm, face, head, neck, torso, waist and legs, etc.), animals and objects.
As a coating method, a conventionally known coating method of a biofilm removing agent can be adopted. Specific examples include (i) a method of extruding a biofilm remover contained in a container from a container and foaming it, and (ii) a method of rubbing the biofilm remover by hand and foaming it.
The temperature of the biofilm removing agent is not particularly limited and may be, for example, 15 ° C or higher and 45 ° C or lower.
The application amount of the biofilm removing agent is not particularly limited, and may be appropriately determined depending on the area where the biofilm is removed.

In the removal step, the object to be treated is washed to remove the biofilm.
Water is usually used to wash the object to be treated. Examples of water include purified water, physiological saline and tap water.
The temperature of water is preferably 15 ° C. or higher and 40 ° C. or lower, and more preferably 25 ° C. or higher and 35 ° C. or lower, from the viewpoint of being suitable for humans.

  As described above, according to the biofilm removing method of the present embodiment, the biofilm can be removed using the biofilm removing agent. Further, since the biofilm removing agent of the present embodiment has a small load on the human body, the biofilm removing method of the present embodiment can remove the biofilm with a method having a small load on the human body.

  Next, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the present invention is not limited to these Examples.

[Example 1]
Purified water (78.7 g) was added to the reaction tank, and then potassium hydroxide (100% by mass) (4.2 g) was added to the reaction tank. The reaction tank was placed in a warm bath at 70 ° C., and oleic acid ( 7.9 g of Tokyo Chemical Industry Co., Ltd., net content: 87% by mass) and 9.2 g of lauric acid (Tokyo Kasei Industry Co., Ltd., net content: 99% by mass or more) were added and reacted. Then, it cooled and the biofilm removal agent (80 mass% of water, 20 mass% of fatty-acid potassium) was obtained.
In the obtained biofilm removing agent, the content of potassium fatty acid was 20% by mass based on the total amount of the biofilm removing agent. The content of the pure component (A) was 41.5% by mass based on the total amount of the pure components (A) and (B). Furthermore, the total amount of pure components (A) and (B) was 94% by mass based on the total amount of potassium fatty acid. Table 1 shows the content of fatty acid potassium, the content of the pure component (A), and the total amount of the pure components (A) and (B) in Example 1. Table 1 shows the fatty acid composition (mixing ratio of each fatty acid to the total amount of fatty acids) in Example 1.

[Examples 2 to 17 and Comparative Examples 1 to 13]
Except that the content of fatty acid potassium, the content of the pure component (A), and the total amount of the pure components (A) and (B) are as shown in Table 1, respectively. A biofilm remover was obtained in the same manner as in Example 1. In Example 9, potassium caprate is used as the component (B), but in Examples 1 to 8 and 10 to 18, potassium laurate is used as the component (B).
Further, the content of fatty acid potassium, the content of the pure component (A), and the total amount of the pure components (A) and (B) were set as shown in Table 2, respectively. A biofilm remover was obtained in the same manner as in Example 1 except for the above.
In addition, in Examples 2 to 17 and Comparative Examples 1 to 13, the following fatty acids were used. The fatty acid compositions in Examples 2 to 17 are shown in Table 1. In addition, Table 2 shows the fatty acid composition in Comparative Examples 1 to 13.
Oleic acid (manufactured by Tokyo Chemical Industry Co., Ltd., net content: 87% by mass)
Lauric acid (manufactured by Tokyo Chemical Industry Co., Ltd., net content: 99% by mass or more)
Capric acid (decanoic acid, manufactured by Fujifilm Wako Pure Chemical Industries, Ltd., net content: 98% by mass)
Palmitic acid (manufactured by Tokyo Chemical Industry Co., Ltd., net content: 99% by mass or more)

[Evaluation of biofilm remover]
Evaluation of the biofilm removing agent (biofilm removing property, foaming / foaming stability, liquid stability, stability over time, skin irritation) was performed by the following methods. The results obtained for the examples are shown in Table 1. Table 2 shows the results obtained for the comparative examples.
(1) Biofilm removal property A biofilm removal agent was used as a sample, and the biofilm removal rate was measured by the following test method. Then, the biofilm removability was evaluated according to the following criteria based on the biofilm removal rate.
◯: The removal rate of the biofilm is 75% or more.
X: The removal rate of the biofilm is less than 75%.
(Production of biofilm)
The subcultured slant (OJ-1, ATCC No. BAA-2856) was precultured at 37 ° C. for 24 hours in TSA (Trypticase soy agar) slant medium. Next, the bacteria were scraped from the TSA slant medium and diluted with TSB (Tryptic Soybean Broth) so that the absorbance at a wavelength of 600 nm was 0.1 to obtain a bacterial solution. Next, the bacterial solution was dispensed into wells (cell culture plate 12 well manufactured by Corning, for cultured cells) in an amount of 1 mL each. Next, static culture was carried out at a temperature of 37 ° C. for 24 hours to form a biofilm. A well in which the bacterial solution was cultured without being dispensed was also prepared (this well is used for calculating the removal rate).
(Removal of biofilm)
The supernatant of the biofilm was removed, and 1 mL of sterile physiological saline was dispensed and removed (hereinafter referred to as washing) once. Next, 1 mL of the sample immediately vortexed for several seconds was dispensed into each well. Next, it was allowed to stand at room temperature for 1 minute. Next, the test substance was removed and washed 3 times with 1 mL of sterile physiological saline. The same operation was performed for each sample with N = 3. Instead of the sample, a sterile physiological saline solution was dispensed and used as a negative control (this well is used for calculating the removal rate).
(Measurement of biofilm removal rate)
0.5 mL of a 0.1 mass% crystal violet aqueous solution was dispensed, and the mixture was allowed to stand for 1 hour. Next, the supernatant was removed, and the cells were washed twice with 1 mL of sterilized physiological saline, and thereafter, 2 mL each of 99.5% by volume ethanol was dispensed. Next, the insolubilized deposit on the bottom of the well was scraped off with a plastic Corning rod and allowed to stand for 1 hour to extract crystal violet. Next, it was transferred to a new plate and the absorbance at a wavelength of 570 nm was measured using a plate reader.
The absorbance (Ac) of the well after contact with the sterilized physiological saline, the absorbance (Ab) of the well cultured without the bacterial solution, and the absorbance (As) of the well after contact with the sample were measured. Then, the removal rate of the biofilm was calculated from these measured values based on the following formula (F1).

(2) Foaming and foam retention Using the biofilm remover as a sample, about 0.5 g of the sample was put in a prewashed hand, and the sample was bubbled according to the procedure for washing the normal hand, and the state was observed. Then, foaming and foam retention were evaluated according to the following criteria.
◯: Sufficient foaming and good foam retention.
Δ: The amount of bubbles is small, but bubbles are generated and can be used.
X: Not sufficiently foamed.
(3) Liquid stability The state when the biofilm removing agent was produced or the state when 24 hours had passed after the production was visually confirmed. Then, the stability of the liquid was evaluated according to the following criteria.
◯: There is no precipitation and it is liquid and fluid.
(Triangle | delta): Although a part gelatinizes, it will become liquid after 24 hours and can be used.
X: Fatty acid potassium is deposited, or the whole or part of the gel is formed and does not become liquid even after 24 hours.
(4) Stability over time Using a biofilm remover as a sample, 2.5 g of the sample was placed in a glass vial and stored in a 60 ° C incubator for 3 days, and a 1 ° C incubator for 3 days The appearance of the sample was visually compared. Then, the stability of the liquid was evaluated according to the following criteria.
◯: The appearance of each sample is the same or a slight change in color tone.
Δ: A clear change in color tone is observed, but it can be used.
X: The color tone changed remarkably.
(5) Skin irritation Using a biofilm remover as a sample, about 0.5 g of the sample was placed in a prewashed hand, and the sample was whipped according to the procedure for normal hand washing. Then, the hands were thoroughly rinsed with tap water at about 35 ° C. to wipe off water. Then, the skin irritation was evaluated according to the following criteria.
◯: No irritation to the skin is felt within 1 minute after wiping off water.
X: Irritation to the skin is felt within 1 minute after wiping off water.

  As shown in Table 1 and Table 2, the biofilm removing agents obtained in Examples 1 to 17 are all biofilm removing properties, foaming and foam retention, liquid stability, temporal stability, and skin irritation. It turned out to be good. From this, it was confirmed that the biofilm remover containing potassium oleate has excellent biofilm removal performance and a small load on the human body.

  The biofilm removing agent of the present invention is useful as a technique for removing a biofilm.

Claims (3)

A biofilm remover consisting of water and potassium fatty acid,
The fatty acid potassium contains (A) potassium oleate and (B) at least one selected from the group consisting of potassium laurate and potassium caprate,
The content of the fatty acid potassium is 6% by mass or more and 34% by mass or less based on the total amount of the biofilm removing agent,
The content of the pure component (A) is 6% by mass or more and 85% by mass or less based on the total amount of the pure components (A) and (B).
The total amount of the pure components (A) and (B) is 75% by mass or more based on the total amount of the fatty acid potassium. The biofilm remover according to claim 1, wherein
A biofilm remover characterized by containing no organic solvent. A biofilm removal method using the biofilm removal agent according to claim 1 or 2, wherein
A step of applying the biofilm remover to the object to be treated,
A step of washing the article to which the biofilm remover is applied to remove the biofilm,
A method for removing biofilm, comprising: