JP2020054392A - Culture bag and culture device - Google Patents

Abstract

To sufficiently stir a culture solution while suppressing damage to the culture target in the culture solution.SOLUTION: A deformable culture bag culturing while containing a culture solution has a bellows shape that is expandable and contractable in a first direction, by dividing the inner space of the culture bag by the partition portion arranged in the bellows-shaped expanded portion of the inner space of the culture bag, a first culture space and a second culture space being defined to be aligned in the first direction, the partition portion having a through hole as a flow path that connects the first culture space and the second culture space and allows the culture solution to come and go.SELECTED DRAWING: Figure 9

Description

  The present invention relates to a culture bag for performing culture and a culture device using the culture bag.

  Conventionally, disposable culture bags have been used for culturing microorganisms, animal and plant tissues, and the like. The culture bag is a foldable bag, as described in Patent Document 1, for example, in which a culture solution and a culture target (for example, cells) are stored. The culture bag includes a port for supplying a gas such as oxygen or carbon dioxide to the inside.

  The culture bag described in Patent Literature 1 includes a stirring blade that stirs a culture solution in the culture bag. Specifically, the culture bag includes a bearing fixed to the culture bag, and a rotation shaft extending from the outside to the inside of the culture bag rotatably supported by the bearing. A stirring blade is attached to one end of a rotating shaft located in the culture bag. The other end of the rotating shaft located outside the culture bag is connected to a motor. When the motor rotates the rotating shaft, the stirring blade rotates inside the culture bag, whereby the culture solution in the culture bag is stirred.

JP 2007-282629 A

  However, in the case of the culture bag described in Patent Literature 1, nutrients are supplied to the culture target by rotation (stirring) of the stirring blade, but a large shear force is generated in the culture solution by the rotation of the stirring blade, and the shear force is increased. May damage the culture target. If the rotation speed of the stirring blade is reduced to reduce the shearing force, the nutrient supply amount by stirring cannot keep up with the nutrient consumption of the culture target, and the culture target may be damaged by lack of nutrition. That is, when using the stirring blade, there is a trade-off relationship between the shearing force generated by the rotation and the nutrient supply amount.

  Therefore, an object of the present invention is to sufficiently agitate a culture solution while suppressing damage to a culture target in the culture solution.

In order to solve the above technical problems, according to one embodiment of the present invention,
A deformable culture bag containing a culture solution and performing culture,
The culture bag is
A bellows shape that can expand and contract in a first direction,
The inner space of the culture bag is divided by the partitioning portion arranged in the bellows-shaped enlarged portion of the inner space of the culture bag, so that the first culture space and the second culture space are divided into the first culture space and the first culture space. It is defined to line up in the direction,
The partition is
Having a through-hole as a flow path, which allows the first culture space and the second culture space to communicate with each other to allow a culture solution to flow,
A culture bag is provided.

According to another aspect of the present invention,
A culture bag containing a culture solution and culturing,
A culture bag deforming part for deforming the culture bag,
A culture device comprising:
The culture bag is
A bellows shape that can expand and contract in a first direction,
The inner space of the culture bag is divided by the partitioning portion arranged in the bellows-shaped enlarged portion of the inner space of the culture bag, so that the first culture space and the second culture space are divided into the first culture space and the first culture space. It is defined to line up in the direction,
The partition is
A first culture space and a second culture space which communicate with each other and allow a culture solution to flow therethrough;
The culture bag deformation part is
By moving the partition and deforming the culture bag, the culture solution is moved between the first and second culture spaces through the through hole of the partition,
A culture device is provided.

  ADVANTAGE OF THE INVENTION According to this invention, a culture solution can be agitated sufficiently, suppressing damage to a culture object.

1 is a perspective view of a culture bag according to Embodiment 1 of the present invention. Sectional view of the culture bag shown in FIG. Schematic diagram of an example of a culture device using the culture bag shown in FIG. Sectional drawing of the culture bag shown in FIG. 1 in the state where the second culture space was reduced and deformed. Sectional view of the culture bag shown in FIG. 1 in a state where the first culture space is reduced and deformed. Sectional view of the culture bag shown in FIG. 1 in a folded state Sectional view of a culture bag for explaining different stirring methods of a culture solution Sectional view of a further culture bag for explaining different stirring methods of the culture solution Cross-sectional view of a culture bag for explaining a different method of stirring a culture solution Sectional view of the culture bag according to Embodiment 2 of the present invention. Sectional view of culture bag according to Embodiment 3 of the present invention Sectional view of culture bag according to Embodiment 4 of the present invention Sectional view of the culture bag according to Embodiment 5 of the present invention. Sectional view of the culture bag shown in FIG. 11 in a state where the second culture space is reduced and deformed. Sectional view of the culture bag according to Embodiment 6 of the present invention. A perspective view of a culture bag according to Embodiment 7 of the present invention.

  The culture bag of one embodiment of the present invention is a deformable culture bag that accommodates a culture solution and performs culture, and includes a first culture space defined by an inner surface of the culture bag and an inner surface of the culture bag. It has a defined second culture space different from the first culture space, and a flow path through which the first culture space and the second culture space can communicate with each other and through which a culture solution can flow.

  According to this aspect, the culture solution can be sufficiently stirred while suppressing damage to the culture target.

  The culture bag defines a first culture space and a second culture space by dividing an internal space of the culture bag, and serves as a flow path that communicates the first culture space and the second culture space. May be provided. This makes it possible to sufficiently agitate the culture solution while suppressing damage to the culture target using a culture bag having a simple configuration.

  The culture bag may be configured to be expandable and contractible in a first direction, and a partition may be provided in the internal space of the culture bag so that the first and second culture spaces are arranged in the first direction. Thus, the culture solution moves between the first culture space and the second culture space by expansion and contraction of the culture bag in the first direction.

  The culture bag may be formed in a bellows shape that can expand and contract in the first direction. In this case, even if the entire culture bag is largely deformed, the neck portion of the bellows-shaped culture bag is small in deformation compared to other portions. It is preferable to arrange the partition portion at the center.

  The culture bag that can be expanded and contracted in the first direction is larger in size in the second direction and the third direction that are orthogonal to the first direction and orthogonal to each other than the size of the culture bag in the first direction. Is more preferred. Thus, a large amount of culture solution can be sufficiently stirred by expanding and contracting the culture bag in the first direction with a small stroke amount.

  A culture device according to another aspect of the present invention is a deformable culture bag for containing and culturing a culture solution, wherein the first culture space is defined by an inner surface of the culture bag, and is defined by an inner surface of the culture bag. A second culture space that is different from the first culture space, a flow passage that allows the culture solution to flow through the first culture space and the second culture space, and a culture bag that is deformed. And a culture bag deforming section that allows a culture solution to flow between the first and second culture spaces via the flow path.

  According to this aspect, the culture solution can be sufficiently stirred while suppressing damage to the culture target.

  The culture bag defines a first culture space and a second culture space by dividing an internal space of the culture bag, and serves as a flow path that communicates the first culture space and the second culture space. May be provided. In this case, the culture bag deforming section may move the partition to deform the culture bag, thereby allowing the culture solution to flow between the first and second culture spaces through the through hole of the partition. . This makes it possible to sufficiently agitate the culture solution while suppressing damage to the culture target using a culture bag having a simple configuration.

  The culture bag may be configured to be expandable and contractible in a first direction, and a partition may be provided in the internal space of the culture bag so that the first and second culture spaces are arranged in the first direction. Thus, the culture solution moves between the first culture space and the second culture space by expansion and contraction of the culture bag in the first direction.

  The culture bag may be formed in a bellows shape that can expand and contract in the first direction. In this case, even if the entire culture bag is largely deformed, the neck portion of the bellows-shaped culture bag is small in deformation compared to other portions. It is preferable to arrange the partition portion at the center.

  The culture bag that can be expanded and contracted in the first direction is larger in size in the second direction and the third direction that are orthogonal to the first direction and orthogonal to each other than the size of the culture bag in the first direction. Is more preferred. In this case, the culture bag deformation unit deforms the culture bag in the first direction. Thus, a large amount of culture solution can be sufficiently stirred by expanding and contracting the culture bag in the first direction with a small stroke amount.

  The culture device may include a culture bag position / posture changing unit that changes at least one of the position and the posture of the culture bag to generate a flow of the culture solution in the first and second culture spaces. Thereby, the culture solution in the culture bag is further stirred.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

(Embodiment 1)
FIG. 1 schematically shows a culture bag according to Embodiment 1 of the present invention. FIG. 2 is a sectional view of the culture bag shown in FIG. Although an XYZ orthogonal coordinate system is shown in the drawings, this is for the purpose of facilitating the understanding of the embodiments of the present invention, and does not limit the present invention. The X-axis direction and the Y-axis direction are horizontal directions, and the Z-axis direction is a vertical direction.

  As shown in FIGS. 1 and 2, the culture bag 10 is configured to store therein a culture solution L containing a culture target such as cells. The culture bag 10 is also deformable, and in the case of the first embodiment, has a bellows shape that can expand and contract in the Z-axis direction (first direction).

  Specifically, the culture bag 10 is made of a deformable material such as a resin material, and has a bottomed cylindrical culture solution storage unit 12 having a circular cross section for storing the culture solution L; It has a disk-shaped top plate 14 and a bottom plate 16 attached to both ends in the Z-axis direction.

  The culture solution container 12 has a bellows shape that can expand and contract in the Z-axis direction, and is made of a transparent resin material so that the inside thereof can be viewed. Specifically, the bellows-shaped culture solution accommodating portion 12 includes a plurality of enlarged diameter portions 12a having a relatively large circular cross section centered on a central axis C extending in the Z-axis direction, and a plurality of enlarged diameter portions 12a. And a reduced diameter portion 12b having a relatively small circular cross section.

  As shown in FIG. 2, a culture solution L containing a culture target such as cells is stored in an internal space 12 c of the culture solution storage unit 12. The top plate 14 is provided with a sampling port 18 for introducing the culture solution L into the internal space 12c or collecting a sample to be cultured during the culture in the internal space 12c. The sampling port 18 is openable and closable, and when opened, connects the internal space 12c of the culture solution container 12 with the outside.

  In addition to the sampling port 18, an intake port 20 for supplying gas such as oxygen to the internal space 12c of the culture solution storage unit 12 and an exhaust port 22 for discharging gas in the internal space 12c to the outside are provided. , On the top plate 14. The intake port 20 is connected to an external gas supply source (not shown). During the culture, gases such as oxygen and carbon dioxide required for the culture are supplied from the gas supply source to the internal space 12 c of the culture solution storage unit 12 via the intake port 20.

  As shown in FIG. 2, the internal space 12c of the culture solution container 12 is divided into a plurality of culture spaces 12d and 12e. In the case of the first embodiment, the internal space 12c is divided into a first culture space 12d and a second culture space 12e. Specifically, the first and second culture spaces 12d and 12e are arranged in the direction in which the center axis C of the culture solution storage unit 12 extends (the Z-axis direction), that is, in the direction in which the culture solution storage unit 12 expands and contracts. I have. The first culture space 12d is arranged on the top plate 14 side, and the second culture space 12e is arranged on the bottom plate 16 side.

  As shown in FIG. 2, in order to divide the internal space 12c of the culture solution storage unit 12 into first and second culture spaces 12d and 12e, a partition 24 is provided in the internal space 12c.

  The partition part 24 is provided in the reduced diameter part 12b of the culture solution accommodating part 12 where the deformation is relatively small, and is a disk-shaped part made of a deformable material such as a resin material as shown in FIG. It is. In addition, you may manufacture the culture-solution accommodating part 12 and the partition part 24 from the same material, and thereby may integrate these.

  The partition 24 defines a different first culture space 12 d and a different second culture space 12 e in the culture solution storage unit 12. That is, the first culture space is formed by a part of the inner surface of the culture solution accommodating portion 12 (the inner surface on the top plate 14 side with respect to the reduced diameter portion 12b) and the partition portion 24 (the surface on the top plate 14 side). 12d is defined. The second culture space 12e is formed by the remaining inner surface of the culture solution storage unit 12 (the inner surface on the bottom plate 16 side with respect to the reduced diameter portion 12b) and the partitioning unit 24 (the surface on the bottom plate 16 side). Is defined.

  As shown in FIGS. 1 and 2, at least one through-hole 24 a is formed in the partition part 24 as a flow path for the culture solution L to flow between the first culture space 12 d and the second culture space 12 e. Is provided. In the case of the first embodiment, a plurality of through holes 24a are provided in the partition part 24. Further, in the case of the first embodiment, the plurality of through holes 24a extend in the direction of expansion and contraction (the Z-axis direction) of the culture solution storage unit 12.

  FIG. 3 schematically shows an example of a culture apparatus for performing culture using the culture bag 10.

  As shown in FIG. 3, an example of the culture apparatus 50 includes a stage 52 on which the culture bag 10 is placed, and a top plate 14 of the culture bag 10 which is held at a predetermined height from the stage 52. And a bottom chuck 56 for fixing the bottom plate 16 to the stage 52.

  The culture device 50 also has an elevating arm 58 for deforming the culture bag 10 (the culture solution storage unit 12 thereof) fixed to the stage 52 via the top chuck 54 and the bottom chuck 56.

  Specifically, the elevating arm 58 holds the partition part 24 of the culture bag 10 (in the case of the first embodiment, the reduced diameter part 12b of the culture solution storage part 12) in the Z-axis direction (ie, The culture bag 10 is configured to be deformed by reciprocating in the direction of expansion and contraction of the culture solution storage unit 12). For example, the lift arm 58 is moved up and down via a ball screw 60 that engages with the lift arm 58 and a motor 62 that rotates the ball screw 60. The culture bag 10 is deformed by the lifting arm 58 such that the partition 24 approaches the top plate 14 and the partition 24 approaches the bottom plate 16.

  As shown in FIG. 4A, when the culture bag 10 is deformed by the lifting arm 58 so that the partition 24 approaches the bottom plate 16 in the downward direction (the direction of the arrow D <b> 1), the position between the partition 24 and the bottom plate 16 is increased. The second culture space 12e of the culture bag 10 is reduced and deformed. On the other hand, the first culture space 12d expands and deforms.

  Due to such a deformation of the culture bag 10, the culture solution L in the second culture space 12 e moves to the first culture space 12 d via the plurality of through holes 24 a of the partition 24. That is, a flow F1 (upflow) of the culture solution L from the second culture space 12e to the first culture space 12d via the through hole 24a is generated. By the flow F1, the culture solution L in the first culture space 12d is stirred throughout. Further, a large amount of gas is taken into the culture solution L at the liquid level Ls of the culture solution L by the flow F1.

  On the other hand, as shown in FIG. 4B, when the culture bag 10 is deformed by the lifting arm 58 so that the partition 24 approaches the top plate 14 in the upward direction (the direction of the arrow D2), the space between the partition 24 and the top plate 14 is changed. Is reduced and deformed in the first culture space 12d of the culture bag 10 located at the position. On the other hand, the second culture space 12e expands and deforms.

  Due to such a deformation of the culture bag 10, the culture solution L in the first culture space 12 d moves to the second culture space 12 e via the plurality of through holes 24 a of the partition 24. That is, a flow F2 (downflow) of the culture solution L from the first culture space 12d to the second culture space 12e via the through hole 24a is generated. By the flow F2, the culture solution L in the second culture space 12e is stirred throughout.

  Therefore, the culture bag 10 is repeatedly deformed by the raising / lowering arm 58 so that the partitioning portion 24 reciprocates between the top plate 14 and the bottom plate 16 in the direction (Z-axis direction) facing these plates, whereby the first The culture solution in each of the second culture spaces 12d and 12e is alternately stirred. At the same time, the culture solution L moves between the first culture space 12d and the second culture space 12e. As a result, the culture solution L in the internal space 12c of the culture solution storage unit 12 of the culture bag 10 is stirred throughout.

  According to the first embodiment, the culture solution L can be sufficiently stirred while suppressing damage to the culture target in the culture solution L.

  Specifically, the culture solution L in the culture bag 10 can be stirred by the deformation of the culture bag 10 without using a stirring blade. Therefore, damage to the culture target due to the shearing force generated in the culture solution due to the stirring of the stirring blade is suppressed.

  Even without the stirring blade, the culture solution is sufficiently stirred by going back and forth through the through hole 24a of the partition 24 that connects the first culture space 12d and the second culture space 12e. That is, the culture solution L in the second culture space 12e moves to the first culture space 12d through the through-hole 24a as shown in FIG. 4A, and the first culture as shown in FIG. 4B. By moving the culture solution L in the space 12d into the second culture space 12e via the through hole 24a, the culture solution L is sufficiently stirred without a stirring blade.

  Therefore, according to the first embodiment, due to the deformation of the culture bag 10, the damage to the culture solution is suppressed as compared with the case where the culture solution is stirred using the stirring blades, and the culture solution can be reduced even without the stirring blades. Can be sufficiently stirred.

  In addition, the deformation of the culture bag 10 is useful not only for stirring the culture solution L therein, but also for storage and disposal. That is, as shown in FIG. 5, the entire culture bag 10 can be reduced in size in the expansion and contraction direction (Z-axis direction) of the culture solution storage unit 12 as compared with the original state (folding is possible). it can). By folding the culture bag 10, storage and disposal become easy.

  In the case of the first embodiment, the size of the culture bag 10 (that is, the internal space 12c of the culture solution storage unit 12) in the horizontal direction (X-axis direction, Y-axis direction) is smaller than the size in the vertical direction (Z-axis direction). Therefore, many culture solutions L can be used for culture.

  Specifically, when a culture target is cultured using many culture solutions L, stirring of the culture solution L becomes important. When the horizontal size of the internal space 12c of the culture solution accommodating portion 12 for accommodating the culture solution L is smaller than the vertical size, in order to sufficiently stir the entire culture solution L inside, the partition portion 24 must be vertically It is necessary to reciprocate with a large stroke in the direction. Therefore, it takes time to move the partition 24 by one stroke. As a result, stirring of a large amount of culture solution may be insufficient.

  On the other hand, when the horizontal size of the internal space 12c of the culture solution accommodating portion 12 is larger than the vertical size as in the first embodiment, the partition portion 24 necessary for sufficiently stirring the entire culture solution L is used. Has a small stroke amount in the vertical direction (compared to the case where the horizontal size is smaller than the vertical size). Therefore, the time required to move the partition 24 by one stroke is short, and as a result, a large amount of the culture solution L can be sufficiently stirred.

  Further, as shown in FIGS. 4A and 4B, the culture bag 10 can be deformed without moving the partitioning part 24 in the parallel direction (Z-axis direction) of the first culture space 12d and the second culture space 12e. It is possible.

  For example, as shown in FIG. 6A and FIG. 6B, the partition part 24 is moved in a horizontal direction orthogonal to the parallel direction (Z-axis direction) of the first culture space 12d and the second culture space 12e, for example, in the X-axis direction. Then, the culture bag 10 may be deformed.

  In this case, the culture solution L extends in an inclined state from the edge of the through hole 24a so that the culture solution L passes through the through hole 24a of the partition portion 24 that moves in the horizontal direction (X-axis direction). An inclined guide portion 24b for guiding the through hole 24a is provided. Thereby, as shown in FIG. 6A, when the partitioning part 24 moves horizontally in the direction of arrow D3 (left direction in the figure), the culture solution L in the second culture space 12e is moved by the inclined guide part 24b into the first culture space. Move into 12d. At this time, the culture solution L flows into the first culture space 12d obliquely with respect to the partition portion 24 along the inclined guide portion 24b. Thereby, a horizontal flow F3 (a flow including a flow component in the X-axis direction and a flow component in the Y-axis direction) is generated in the first culture space 12d. Thereby, the culture solution L in the first culture space 12d is stirred.

  On the other hand, as shown in FIG. 6B, when the partition part 24 moves horizontally in the direction of arrow D4 (right side in the figure), the culture solution L in the first culture space 12d is moved by the inclined guide part 24b into the second culture space 12e. Go to At this time, the culture solution L flows into the second culture space 12e obliquely with respect to the partition portion 24 along the inclined guide portion 24b. Thus, a horizontal flow F4 (a flow including a flow component in the X-axis direction and a flow component in the Y-axis direction) is generated in the first culture space 12d. Thereby, the culture solution L in the second culture space 12e is stirred.

  Furthermore, the moving direction of the partition part 24 for deforming the culture bag 10 is not limited to the vertical direction (Z-axis direction) or the horizontal direction (X-axis direction, Y-axis direction), but also the X-axis direction, Y-axis direction, And an oblique direction including at least two components in the Z-axis direction.

  In addition, the culture bag 10 may be deformed by changing the posture of the partition part 24 instead of or in addition to the parallel movement instead of moving the partition part 24 in parallel. For example, as shown in FIG. 7, the partition 24 may be rotated in the direction of arrow D5 about a rotation center line parallel to the horizontal direction (Y-axis direction).

  In addition, as shown in FIG. 3, the partition 24 is fixed by the top chuck 54 and the bottom chuck 56 instead of displacing the partition 24 relative to the fixed top plate 14 and bottom plate 16. Then, the top plate 14 and the bottom plate 16 may be displaced. Also in this case, the culture bag 10 can be deformed and the culture solution L inside can be stirred.

(Embodiment 2)
The second embodiment differs from the first embodiment in the number of culture spaces in a culture bag. Therefore, the second embodiment will be described focusing on different points. Further, the same reference numerals are given to the same components as those in the first embodiment.

  FIG. 8 is a sectional view of the culture bag according to the second embodiment.

  As shown in FIG. 8, in the culture bag 110, the internal space 112c of the bellows-shaped culture solution storage unit 112 is divided into three spaces (first to third culture spaces) 112d, 112e, and 112f. Specifically, the internal space 112c is divided into three by partitioning parts 124 provided in each of the two reduced diameter parts 112b.

  The first culture space 112d, the second culture space 112e, and the third culture space 112f are arranged in the direction of expansion and contraction (Z-axis direction) of the culture bag 110 (the bellows-shaped culture solution storage unit 112). The first culture space 112d is arranged on the side of the top plate 14, the third culture space 112f is arranged on the side of the bottom plate 16, and the second culture space 112e is composed of the first culture space 112d and the third culture space. 112f. Therefore, the second culture space 112e directly communicates with the first and third culture spaces 112d and 112f through the through hole 124a of the partition 124, but the first culture space 112d and the third culture space 112d. It is not in direct communication with 112f.

  According to such a culture bag 110, for example, by independently moving each of the two partition portions 124, the culture bag 110 can be deformed into various shapes as compared with the first embodiment. . Thereby, it is possible to generate a complicated flow of the culture solution L in the culture bag 110. As a result, depending on the culture target, the stirring is further preferably and appropriately performed, and the culture efficiency can be improved.

  When three or more culture spaces exist as in the second embodiment, that is, when two or more partition portions exist between them, the through holes formed in each of the partition portions are The position and number may be different for each copy. Thus, when the culture bag is deformed, a more complicated flow of the culture solution is formed in the culture bag. As a result, depending on the object to be cultured, stirring is more preferably performed appropriately, and the culture efficiency can be improved.

(Embodiment 3)
The third embodiment differs from the first embodiment in the position of the partition. Therefore, the third embodiment will be described focusing on different points. Further, the same reference numerals are given to the same components as those in the first embodiment.

  FIG. 9 is a cross-sectional view of the culture bag according to the third embodiment.

  As shown in FIG. 9, the culture bag 210 according to the third embodiment includes a first culture space 212d and a second culture space 212e as two culture spaces, as in the first embodiment. Having. That is, the internal space 212 c of the culture solution storage 212 of the culture bag 210 is divided by the partition 224.

  However, in the case of the above-described first embodiment, as shown in FIG. 2, the partition portion 24 is provided at the reduced diameter portion 12 b in the bellows-shaped culture solution storage unit 12. That is, even if the entire culture bag 10 is greatly deformed, the partitioning portion 24 is provided in the reduced diameter portion 12b which changes little compared to other portions, specifically, the cross-sectional area hardly changes.

  On the other hand, in the case of the third embodiment, as shown in FIG. 9, the partition portion 224 is provided not at the reduced diameter portion 212b of the bellows-shaped culture solution storage portion 212 but at the enlarged diameter portion 212a. The partition 224 is made of an elastically deformable material because the partition 224 is provided in the enlarged diameter portion 212a whose cross-sectional area changes greatly due to the deformation of the culture bag 210.

  According to such a partition part 224, the cross-sectional area of the through hole 224a changes with the elastic deformation of the partition part 224 during the deformation of the culture bag 210 (the culture solution storage part 212). Thus, the flow rate of the culture solution L flowing between the first culture space 212d and the second culture space 212e via the through hole 224a during the deformation of the culture bag 210 changes. Thereby, it is possible to generate a complicated flow of the culture solution L in the culture bag 210. As a result, depending on the object to be cultured, stirring is more preferably performed appropriately, and the culture efficiency can be improved.

(Embodiment 4)
The fourth embodiment differs from the first to third embodiments described above in the culture solution storage unit. Therefore, the third embodiment will be described focusing on different points. Further, the same reference numerals are given to the same components as those in the first embodiment.

  FIG. 10 is a sectional view of the culture bag according to the fourth embodiment.

  As shown in FIG. 10, the culture solution container 312 of the culture bag 310 according to Embodiment 4 includes a cylindrical portion 312a and a bellows portion 312b. The cylindrical portion 312a is disposed on the top plate 14 side, and the bellows portion 312b is disposed on the bottom plate 16 side.

  The internal space 312c of the culture solution storage unit 312 is divided into three culture spaces 312d, 312e, and 312f. The first culture space 312d is provided in the cylindrical portion 312a, and the second and third culture spaces 312e and 312f are provided in the bellows portion 312b.

  Since the culture solution storage unit 312 includes the cylindrical portion 312a and the bellows portion 312b, the culture solution storage unit 312 is partially deformed. That is, the cylindrical portion 312a is hardly deformed, and the bellows portion 312b is deformed. Thereby, during the deformation of the culture bag 310, the flow rate of the culture solution L in the first culture space 312d in the cylindrical portion 312a is changed to the culture solution in the second and third culture spaces 312e and 312f in the bellows portion 312b. It is lower than the flow rate of L. As described above, when the flow rate difference of the culture solution L is generated in the internal space 312c of the culture solution storage unit 312, that is, when the pressure difference is generated, the entire culture solution storage unit 312 is in a bellows shape, and the flow rate difference is almost zero. The culture is more agitated than would otherwise be the case.

  In addition, the position of the cylindrical portion 312a in the culture solution container 312 may be anywhere. For example, there may be a cylindrical portion 312a between the bellows portion 312b and the bottom plate 16. Further, for example, a cylindrical portion 312a may be provided between the two bellows portions 312b.

  Also, as shown in FIG. 10, the cylindrical portion 312a is provided at the uppermost position in the culture solution storage portion 312, and the liquid level Ls of the culture solution L is changed during the deformation of the culture bag 310 to the culture space 312d in the cylindrical portion 312a. In addition to the above, when the compound is almost present, another effect is produced in addition to the improvement of the stirring property. Since the flow rate of the culture solution L is lower in the cylindrical portion 312a than in the bellows portion 312b, the liquid level Ls in the cylindrical portion 312a is less likely to undulate. As a result, bubbles that may damage the culture target are less likely to be generated at the interface (liquid level Ls) between the culture solution L and the gas.

(Embodiment 5)
The fifth embodiment is different from the above-described first to fourth embodiments with respect to the culture solution storage unit. Therefore, the fifth embodiment will be described focusing on the different points. Further, the same reference numerals are given to the same components as those in the first embodiment.

  FIG. 11 is a sectional view of the culture bag according to the fifth embodiment.

  In the case of the first embodiment, as shown in FIG. 2, the culture solution storage section 12 of the culture bag 10 has a bellows shape. On the other hand, the culture solution storage section 412 of the culture bag 410 according to Embodiment 5 has a folding lantern structure.

  Specifically, the culture solution storage section 412 of the culture bag 410 is made of a deformable material and has a bottomed cylindrical main body 412a having a circular cross section, and a main body 412a made of a metal material or a resin material. And a plurality of annular frames 412b provided therein. For example, the main body 412a is made of a thin resin sheet that is easily deformed. The cross section of the culture solution container 412 is maintained in a circular shape by the plurality of frames 412b.

  The internal space 412c of the culture solution storage unit 412 is divided by a partition 424 into a first culture space 412d and a second culture space 412e.

  As shown in FIG. 12, when the bottom plate 16 moves toward the partition 424 in the upward direction (the direction of the arrow D6), the culture solution storage unit 412 is deformed, and the second culture space 412e is reduced and deformed. Thereby, the culture solution L in the second culture space 412e moves to the first culture space 412d through the plurality of through holes 424a of the partition 424. On the other hand, when the top plate 14 moves toward the partition 424, the first culture space 412d is reduced and deformed. Thereby, the culture solution L in the first culture space 412d moves to the second culture space 412e via the through hole 424a. Thereby, the culture solution L in the internal space 412c of the culture solution storage section 412 is stirred.

(Embodiment 6)
The sixth embodiment differs from the fifth embodiment in the frame. Therefore, the sixth embodiment will be described focusing on the different points. Further, the same reference numerals are given to the same components as those in the first embodiment.

  FIG. 13 is a sectional view of the culture bag according to the sixth embodiment.

  As shown in FIG. 13, the culture solution storage portion 512 of the culture bag 510 is formed of a deformable material and has a bottomed cylindrical main body portion 512 a having a circular cross section, and a metal material or a resin material formed of a main body. And a frame 512b provided in the portion 412a. For example, the main body 512a is made of a thin resin sheet that is easily deformed.

  In the case of the sixth embodiment, the number of the frames 512b is one, and is in a helical coil shape. By the helical coil-shaped frame 512b, the shape of the culture solution storage section 412 is maintained in a cylindrical shape (the cross section is maintained in a circular shape). That is, the culture bag 510 can be self-supported by the frame 512b while maintaining the cylindrical shape.

(Embodiment 7)
The seventh embodiment is different from the above-described first to sixth embodiments with respect to the culture solution storage unit. Therefore, the seventh embodiment will be described focusing on the different points. Further, the same reference numerals are given to the same components as those in the first embodiment.

  FIG. 14 is a perspective view of the culture bag according to the seventh embodiment.

  In the above-described first to sixth embodiments, for example, as shown in FIG. 1, the culture solution storage unit 12 of the culture bag 10 according to the first embodiment has a bottomed cylindrical shape having a circular cross section, and has an enlarged diameter. It has a bellows shape including a portion 12a and a reduced diameter portion 12b.

  On the other hand, in the case of the seventh embodiment, as shown in FIG. 14, the culture solution storage section 612 of the culture bag 610 has a bottomed cylindrical shape having a rectangular cross section or a square cross section, and has a large diameter section 612a. It has a bellows shape with a reduced diameter portion 612b.

  In the culture bag 610 according to the seventh embodiment, since the cross section of the culture solution storage unit 612 is rectangular or square, the cross section of the culture solution storage unit 12 is circular when the footprints are the same. Can accommodate a larger amount of culture liquid L than the culture bag 10 of FIG.

  The cross-sectional shape of the culture solution storage section of the culture bag is not limited to a square or a rectangle, and is not limited to a circle, and may be another shape. Further, the cross-sectional shape of the culture solution container may be different depending on the position in the vertical direction.

  As described above, the present invention has been described with reference to the above-described plurality of embodiments 1 to 7, but the embodiment of the present invention is not limited thereto.

  For example, in the case of the culture bag of the above-described embodiment, the culture solution inside the culture bag is stirred by being deformed. In addition, the flow of the culture solution may be generated in the first and second culture spaces of the culture bag by changing at least one of the position and the posture of the culture bag. In this case, the culture device includes, for example, a culture bag deforming unit (for example, the elevating arm 58 shown in FIG. 3) that deforms the culture bag, and a culture bag position and posture changing unit (for example, FIG. 3) that changes the position and posture of the culture bag. (A stage swinging unit for swinging the stage 52).

  Thereby, the culture solution is further agitated as compared with the case where the culture solution in the culture bag is agitated only by the deformation of the culture bag.

  Further, in the above-described embodiment, the flow path that connects the first culture space and the second culture space is a through hole that is always open, but is not limited thereto. For example, the flow path that connects the first culture space and the second culture space is a valve that opens when the pressure in the first culture space or the second culture space exceeds a predetermined value due to deformation of the culture bag. You may.

  Furthermore, in the case of the above-described embodiment, the culture bag (the culture solution storage portion) expands and contracts in the vertical direction (Z direction), but the expansion and contraction direction of the culture bag is not limited to this. For example, the culture bag may expand and contract in the horizontal direction.

  Furthermore, in the case of the above-described embodiment, the first culture space and the second culture space are provided by dividing the internal space in the culture bag by the partition. In the culture bag, in particular, the culture solution storage portion has a bellows shape or a folding lantern shape. However, embodiments of the present invention are not limited to this. For example, the culture bag is provided with a constricted portion, and an inner space thereof is divided into a first culture space and a second culture space by the constricted portion, and the first culture space and the second culture space are further divided in the constricted portion. It may be a culture bag communicated by a space (flow path). That is, the culture bag of the present invention is, in a broad sense, a deformable culture bag that accommodates a culture solution and performs culture, and includes a first culture space defined by the inner surface of the culture bag and a culture bag. It has a second culture space defined by the inner surface, which is different from the first culture space, and a flow path through which the first culture space and the second culture space can communicate with each other and through which a culture solution can flow.

  The present invention is applicable to culture using a culture bag.

Reference Signs List 10 culture bag 12d first culture space 12e second culture space 24a channel (through hole)

Claims (4)

A culture bag containing a culture solution for culturing,
  The culture bag is
  A bellows shape that can expand and contract in a first direction,
  The inner space of the culture bag is divided by the partitioning portion arranged in the bellows-shaped enlarged portion of the inner space of the culture bag, so that the first culture space and the second culture space are divided into the first culture space and the first culture space. It is defined to line up in the direction,
  The partition is
Having a through-hole as a flow path, which allows the first culture space and the second culture space to communicate with each other to allow a culture solution to flow,
Culture bag.
A culture bag containing a culture solution and culturing,
A culture bag deforming part for deforming the culture bag,
A culture device comprising:
The culture bag is
A bellows shape that can expand and contract in a first direction,
The inner space of the culture bag is divided by the partitioning portion arranged in the bellows-shaped enlarged portion of the inner space of the culture bag, so that the first culture space and the second culture space are divided into the first culture space and the first culture space. It is defined to line up in the direction,
The partition is
A first culture space and a second culture space which communicate with each other and allow a culture solution to flow therethrough;
The culture bag deformation part is
By moving the partition and deforming the culture bag, the culture solution is moved between the first and second culture spaces through the through hole of the partition,
Culture device.
The size of the culture bag in the second direction and the third direction orthogonal to each other in the first direction and orthogonal to each other is larger than the size in the first direction,
The culture device according to claim 2, wherein the culture bag deforming section deforms the culture bag in a first direction.
  The culture device according to claim 2 or 3, further comprising a culture bag position / posture changing unit that changes at least one of the position and the posture of the culture bag to generate a flow of the culture solution in the first and second culture spaces.

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