JP2019219256A - Method of assessing atopic dermatitis-related skin condition - Google Patents

Abstract

To provide an atopic dermatitis assessment method which allows for non-invasively, simply, and appropriately assessing atopic dermatitis-related skin condition.SOLUTION: An atopic dermatitis-related skin condition assessment method disclosed herein uses acetone concentration in gas volatilizing from a skin surface of a subject as an indicator to assess atopic dermatitis-related skin condition of the subject.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a method for evaluating a skin condition related to atopic dermatitis, and a system for evaluating a skin condition related to atopic dermatitis.

  The health of the skin, such as the presence or absence and severity of atopic dermatitis, can be evaluated to some extent by visual observation of the skin and image analysis of the skin surface. In addition, it has been proposed to analyze the lipid components constituting the stratum corneum of the skin to evaluate the current state of the subject's health, predict future skin health, and predict possible skin diseases in the future. (See Patent Document 1).

At present, as a method for objectively determining the severity of atopic dermatitis, a test method using blood TARC (thymus and activation-regulated chemokine) as an index is used. TARC is a type of chemokine. It is considered that TARC induces Th2 cells to a local part of a lesion and enhances an allergic reaction, thereby being involved in the pathogenesis of atopic dermatitis and exacerbating the symptoms. It is known that the blood TARC value increases when the severity score of atopic dermatitis is high, and the blood TARC value decreases when the symptoms of atopic dermatitis subside due to the use of skin external preparations. (See Non-Patent Document 1).
However, a test method using a blood TARC value as an index is an invasive method because blood collection is required. Further, there is a problem that it takes time until a result is obtained.

Skin gas has attracted attention as an index for evaluating human health. Skin gas is a gas that is volatilized and released from the surface of the skin, and is produced in association with life activity and contains a low molecular weight organic compound or an inorganic compound as a constituent. Since the collection of skin gas is non-invasive and simple, skin gas as an index for evaluating a health condition is expected to be applied to diagnostic techniques in the healthcare and medical fields. For example, it has been proposed to measure the concentration of acetone contained in skin gas in order to evaluate the burning state of body fat (see Patent Documents 2 and 3). Further, in order to perform early diagnosis of Parkinson's disease, it has been proposed to measure the ratio of benzyl alcohol to benzoate in skin gas (see Patent Document 4).
However, research on skin gas is still young and basic research has not been done much. In particular, there has been little report on the relationship between skin gas and skin properties.

International Publication No. 2017/168902 WO 2013/038959 JP 2014-10046 A JP-A-2015-55620

Journal of Allergy and Clinical Immunology, 2001, vol. 107 (3), pp. 535-541

An object of the present invention is to provide a method for evaluating a skin condition related to atopic dermatitis, which non-invasively, simply, and accurately evaluates a skin condition related to atopic dermatitis.
Further, the present invention can non-invasively, easily and accurately evaluate skin conditions related to atopic dermatitis, and can be suitably used in the above-described method for evaluating skin conditions related to atopic dermatitis. An object of the present invention is to provide a system for evaluating skin conditions related to atopic dermatitis.

The present inventor has conducted intensive studies in view of the above problems. Specifically, we focused on skin gas that evaporates and is released from the skin surface, and examined it as an index indicating the health condition of human skin. As a result, among various low molecular organic compounds and inorganic compounds contained in skin gas, acetone was found to have a high correlation with atopic dermatitis. Then, they found that the skin condition related to atopic dermatitis can be evaluated non-invasively, simply and accurately by using the amount of acetone in skin gas as an index.
The present invention has been completed based on these findings.

The present invention relates to a method for evaluating a skin condition related to atopic dermatitis of a subject, using the amount of acetone in a gas volatilized from the skin surface of the subject as an index, and a method for evaluating a skin condition related to atopic dermatitis. .
Furthermore, the present invention measures the amount of acetone in the gas volatilized from the skin surface of the subject, measuring means, and using the measured amount of acetone as an index to evaluate the skin condition related to atopic dermatitis of the subject, And a calculating means, comprising: a skin condition evaluation system related to atopic dermatitis.

ADVANTAGE OF THE INVENTION According to the evaluation method of the skin condition of this invention, the skin condition related to atopic dermatitis can be simply and accurately evaluated non-invasively.
Further, according to the skin condition evaluation system of the present invention, a skin condition related to atopic dermatitis can be evaluated non-invasively, simply and accurately, and is preferably used in the above-described skin condition evaluation method. Can be.

FIG. 1A is a flowchart illustrating an embodiment of the present invention. FIG. 1B is a flowchart illustrating a preferred embodiment of the present invention. It is a graph which shows the result of having compared the relative density | concentration of acetone in skin gas between a healthy subject group and an atopic dermatitis patient group. 4 is a graph showing the correlation between the relative concentration of acetone in skin gas and the blood TARC value.

As shown in FIG. 1 (A), the method for evaluating a skin condition related to atopic dermatitis according to the present invention comprises acetone contained in a gas volatilized from the skin surface of a subject (hereinafter, also referred to as “skin gas”). The amount is measured (S1), and the subject's skin condition related to atopic dermatitis is evaluated using the measured amount of acetone as an index (S2). In the present invention, since the amount of a component as an index for evaluating the skin condition related to atopic dermatitis is measured from skin gas, the skin condition related to atopic dermatitis is evaluated non-invasively and simply. it can. Furthermore, the skin condition related to atopic dermatitis can be accurately evaluated by using a specific component as an index among various low-molecular organic compounds and inorganic compounds contained in skin gas.
The system for evaluating a skin condition related to atopic dermatitis of the present invention, which will be described later, can be suitably used in the above-described method for evaluating a skin condition related to atopic dermatitis.
Subjects to which the method and system of the present invention can be applied include humans and mammals other than humans. Among them, the method is preferably applied to evaluate a skin condition related to human atopic dermatitis.

  As used herein, “evaluating the skin condition related to atopic dermatitis” means whether or not onset of atopic dermatitis, the possibility of developing atopic dermatitis, the state of prevention of atopic dermatitis, Progress or severity of atopic dermatitis, topical progress or severity of atopic dermatitis, signs of worsening atopic dermatitis, presence or absence of atopic dermatitis tendency (predisposition), cure of atopic dermatitis It refers to evaluating, judging or predicting the condition of the skin related to atopic dermatitis, such as the situation and the therapeutic effect on atopic dermatitis.

In order to easily measure the amount of acetone, it is preferable to collect skin gas (S0) and measure the amount of acetone contained in the collected skin gas (see FIG. 1B).
Skin gas can be collected according to a conventional method. For example, the skin gas is collected by covering the site where the skin gas is collected with a closed space, and collecting the skin gas volatilizing from the skin surface together with the air in the space. For example, skin gas can be collected using a commercially available skin gas collection kit (including a skin gas automatic sampling device, a skin gas collection bag, and the like). Alternatively, skin gas can be collected according to the methods described in JP-A-2010-148692 and JP-A-2014-10046.
The site from which skin gas is collected is not particularly limited as long as it is the body surface, but specifically, the face and neck, such as the forehead and cheeks, palms, elbows, upper arms, trunk, lower limbs, and knees A sampling site may be appropriately selected from these sites according to the purpose of the evaluation. Among these sites, the cheeks, palms, elbows, and knees are preferable, and the palms and elbows are more preferable, from the viewpoint that the actual state of atopic symptoms can be easily grasped and skin gas can be easily collected.
The time for collecting skin gas is preferably 5 minutes or more, more preferably 10 minutes or more, from the viewpoint that if acetone is too short, sufficient acetone cannot be collected and accurate measurement cannot be performed, and if it is too long, the burden on the subject is increased. . Also, it is preferably 30 minutes or less, and more preferably 20 minutes or less.

In the present invention, it is preferable that the site where the skin gas is collected and the site where the skin condition related to atopic dermatitis is evaluated are the same. Therefore, it is preferable to collect skin gas from a site where a skin condition related to atopic dermatitis is to be evaluated or a site near the site.
Specific sites for collecting skin gas include sites where onset of atopic dermatitis (hereinafter also referred to as “rash”) and sites where no symptoms of atopic dermatitis are observed (hereinafter also referred to as “rash-free”) ), And a non-rash area adjacent to the rash area. In addition, it is also possible to use the skin of a healthy person who has not developed atopic dermatitis (hereinafter, also referred to as “healthy part”) as the sampling site. By collecting skin gas from these skin sites, skin conditions related to atopic dermatitis (presence or absence of onset, possibility of onset, progression of disease state, degree of healing, therapeutic effect, etc.) can be determined or predicted . In addition, the analysis of the predisposition (propensity for atopic dermatitis) in healthy subjects who do not develop atopic dermatitis can be predicted without performing genetic analysis or analysis of blood components. The likelihood of onset and the state of prevention can be easily determined.

The method for measuring the amount of acetone in the skin gas can be appropriately selected from ordinary methods. For example, acetone can be separated and the amount of acetone can be measured using a commercially available gas component detection device such as a gas chromatograph. Alternatively, the amount of acetone can be measured according to the methods described in JP-A-2010-148692 (a measuring method using a gas sensor) and JP-A-2014-10046.
In the present invention, the acetone amount in the collected skin gas may be converted from the measured amount of acetone. By using the numerical value converted into the acetone concentration as an index, the skin condition of the subject related to atopic dermatitis can be objectively evaluated.

  In the present invention, the evaluation of atopic dermatitis is preferably performed based on an evaluation criterion set in advance from the association between the measured amount of acetone and a skin condition related to atopic dermatitis. In the present invention, the skin condition of the subject is evaluated based on the amount or concentration of acetone measured from the skin gas based on the evaluation criteria.

The evaluation criteria can be set as follows, but are not limited thereto.
The skin condition related to atopic dermatitis to be evaluated is evaluated by means of visual evaluation, analysis using a biomarker of atopic dermatitis such as TARC, instrumental analysis, and the like. Separately, the amount of acetone is calculated by the method described above. And, based on the evaluation result of the skin condition related to atopic dermatitis and the correlation with the amount of acetone, a reference value suitable for evaluating the skin condition related to atopic dermatitis is determined. Set evaluation criteria.
Specifically, two or more groups are provided in advance according to the skin condition related to atopic dermatitis to be evaluated, and the average value, standard deviation, standard error, etc. of the calculated value of the amount of acetone in each group are set. The reference value may be determined from the statistical data of the above, and the evaluation criteria for the skin condition may be set. For example, A + SD, A + 1 / 2SD, A + 1 / 4SD, A-SD, A-1 / 2SD, and A determined from the average value (A) and the standard deviation (SD) of the amount of acetone in a group having a specific skin condition. A suitable value selected from the group consisting of − / 4 SD or the like is determined as a reference value. Then, based on whether the acetone amount actually measured is larger or smaller than the reference value, an evaluation criterion is set as to whether or not the skin condition is specific, whether the possibility is large or small, and the like. Then, based on the evaluation criteria, the skin condition related to atopic dermatitis of the subject can be evaluated from the amount of acetone in the subject whose skin condition related to atopic dermatitis is to be evaluated.
Evaluation criteria should be set for each subject, each gender, each age of the subject, and each test site, depending on the subject to be evaluated for skin conditions related to atopic dermatitis and the purpose of the evaluation. Is possible.

As will be shown in Examples described later, the amount of acetone contained in skin gas shows a high correlation with atopic dermatitis. The amount of acetone shows a high correlation with the amount of TARC in blood, which is generally used as a marker for determining the severity of atopic dermatitis.
Therefore, the amount of acetone contained in skin gas is an index for evaluating the skin condition related to atopic dermatitis, and by measuring this, it is possible to easily and accurately evaluate the skin condition related to atopic dermatitis. Can be. Furthermore, according to the method for evaluating the skin condition related to atopic dermatitis of the present invention, in the application test of the external preparation for skin, the ingestion test of some functional foods, pharmaceuticals, and quasi-drugs, etc. By measuring the amount of acetone in the skin gas generated by application or ingestion, the effectiveness of the test substance for preventing or improving atopic dermatitis and improving skin quality can be determined. Furthermore, by using the evaluation method or the evaluation system of the present invention, an agent for preventing or improving atopic dermatitis can be screened. In addition, the evaluation method or evaluation system of the present invention can be used to compare and evaluate the effectiveness of a plurality of agents for preventing or improving atopic dermatitis or agents that are expected to prevent or improve.
As used herein, the term "prevention" refers to preventing or delaying the onset of a disease or condition in an individual, or reducing the risk of developing an disease or condition in an individual. As used herein, “improvement” refers to improvement or alleviation of a disease, symptom, or skin condition, prevention or delay of deterioration of a disease, symptom, or skin condition, or reversal of progress of a disease, symptom, or skin condition. , Prevention or delay.

The evaluation system of the skin condition related to atopic dermatitis of the present invention is a measuring means for measuring the amount of acetone contained in the skin gas of the subject, and using the measured amount of acetone as an index, the atopic dermatitis of the subject Calculating means for evaluating a related skin condition.
The details of the means for measuring the amount of acetone are as described in the method for measuring the amount of acetone described above.
Further, it is preferable that the arithmetic unit stores a database in which information on the amount of acetone contained in the skin gas is associated with a skin condition related to atopic dermatitis. Therefore, based on the calculated amount of acetone, a skin condition related to atopic dermatitis of the subject to be evaluated is evaluated based on the association stored in the database. It is preferable that the above-mentioned evaluation criterion is set in the calculating means.
The system of the present invention is a so-called computer, and includes, for example, a CPU (Central Processing Unit), a memory, an input / output interface, and the like, which are interconnected by a bus. The memory is a random access memory (RAM), a read only memory (ROM), a hard disk, a portable storage medium, or the like. The input / output interface is connected to a user interface device such as a display device or an input device. The input / output interface may be connected to a communication device that communicates with another computer via a network.

  It is preferable that the system of the present invention includes gas collecting means for collecting skin gas, and the measuring means measures the amount of acetone contained in the skin gas collected by the gas collecting means. Furthermore, it is preferable that the calculating means evaluates a skin condition related to atopic dermatitis of the subject using the acetone amount calculated by the measuring means as an index.

  With respect to the above-described embodiments, the present invention further provides the following methods for evaluating a skin condition related to atopic dermatitis, a system for evaluating a skin condition related to atopic dermatitis, and screening for a preventive or ameliorating agent for atopic dermatitis A method is disclosed.

<1> A method for evaluating a skin condition related to atopic dermatitis, wherein a skin condition related to atopic dermatitis of a subject is evaluated using an amount of acetone in a gas volatilized from a skin surface of the subject as an index.
<2> A method of measuring the amount of acetone from gas volatilizing from the skin surface of a subject in order to evaluate a skin condition related to atopic dermatitis.

<3> The skin surface of the subject (preferably the face such as the forehead or cheek, neck, palm, elbow, upper arm, trunk, lower limb or knee, more preferably the cheek, palm, elbow or The gas volatilized from the knee, more preferably the palm or elbow) is collected for preferably 5 minutes or more, more preferably 10 minutes or more, preferably 30 minutes or less, and more preferably 20 minutes or less. The method according to the above item <1> or <2>, wherein the amount of acetone contained in the sample is measured.
<4> The method according to <3>, wherein the amount of acetone in the collected gas is converted into an acetone concentration, and the acetone concentration is used as the index.
<5> Two or more groups are provided according to the skin condition related to atopic dermatitis to be evaluated, and a reference value is determined from the statistical data of the amount of acetone in each group, and the evaluation criteria for the skin condition are determined. The method according to any one of <1> to <4>, wherein a skin condition related to atopic dermatitis of the subject is evaluated from the amount of acetone in the subject based on evaluation criteria.

<6> measuring means for measuring the amount of acetone in the gas volatilized from the skin surface of the subject,
Using the measured amount of acetone as an index, to evaluate the skin condition related to the subject's atopic dermatitis, arithmetic means,
An evaluation system for skin conditions related to atopic dermatitis, comprising:

<7> a gas sampling means for sampling gas volatilized from the skin surface of the subject,
The measuring means measures the amount of acetone contained in the gas collected by the gas collecting means,
The system according to <6>.
<8> The system according to <7>, wherein the measuring unit converts the amount of acetone in the collected gas into acetone.
<9> In the arithmetic means, based on a reference value determined from statistical data of the amount of acetone for each of two or more groups provided according to the skin condition related to atopic dermatitis to be evaluated. Any one of the above <6> to <8>, wherein evaluation criteria for the skin condition are set, and the skin condition related to atopic dermatitis of the subject is evaluated from the amount of acetone in the subject based on the evaluation criteria. System.

<10> As skin conditions related to atopic dermatitis, the presence or absence of atopic dermatitis, the possibility of the development of atopic dermatitis, the state of prevention of atopic dermatitis, the progress of atopic dermatitis or Severity, topical progression or severity of atopic dermatitis, signs of worsening atopic dermatitis, presence or absence of atopic dermatitis tendency (predisposition), healing status of atopic dermatitis, or atopic dermatitis The method or system according to any one of <1> to <9> above, wherein a therapeutic effect on is evaluated or predicted.
<11> The method or system according to any one of <1> to <10> above, for evaluating atopic dermatitis in a human or a non-human mammal.
<12> From the rash of atopic dermatitis, the rash of atopic dermatitis, the rash adjacent to the rash of atopic dermatitis, or the healthy part of a subject who does not develop atopic dermatitis The method or the system according to any one of the above <1> to <11>, wherein gas evaporating from the skin surface is collected.

<13> A substance that is a candidate for a preventive or ameliorating agent for atopic dermatitis is applied to the skin of a subject, and the method according to any one of <1> to <12> above is performed, or a system is used. The change in the skin condition of the skin before and after the application of a substance which is a candidate for a preventive or ameliorating agent for atopic dermatitis is confirmed, and a substance having a preventive or ameliorating effect for atopic dermatitis is identified as atopic dermatitis. A method for screening an agent for preventing or ameliorating atopic dermatitis, which is selected as an agent for preventing or ameliorating inflammation.

  Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto.

(Correlation between atopic dermatitis and the amount of acetone in skin gas)
(1) Subjects 27 patients (25-48 years old) with mild to severe atopic dermatitis using topical steroids for the treatment of atopic dermatitis, and 15 healthy volunteers corresponding to their age ( 29-49 years old).

(2) Skin gas collection A skin gas collection bag (PD-01, manufactured by Pico Device) is attached through a subject's arm through a cylindrically crafted skin gas collection bag. Parafilm (PF-2, Bemis) is placed on the upper and lower parts of the elbow. Was wound and fixed to form a closed space. After sealing, the air in the bag was washed by injecting and collecting 100 mL of clean gas (Pure Air G3 grade, manufactured by Taiyo Nissan Co., Ltd.). After the inside of the bag was washed twice and negative pressure was applied to completely remove the air from the bag, 100 mL of clean gas for skin gas collection was injected, and the skin gas was collected by allowing to stand for 10 minutes.
Ten minutes later, the collected skin gas was collected together with clean gas, transferred to another analysis bag, and used for analysis.

(3) Analysis of the amount of acetone in the skin gas The amount of acetone in the skin gas was analyzed by GC / MS (GCMS-QP2010 manufactured by Shimadzu Corporation) connected to a low-temperature concentrator (NIT-P type, manufactured by Pico Device). Was carried out.
Before the analysis, the analysis bag containing the sample sample was heated at 50 ° C. for 3 minutes, and then 25 mL of the sample was injected into the analyzer. The column used was DB-WAXETR (length 30 m, inner diameter 0.32 mm, film thickness 1 μm, manufactured by Agilent Technology), the trap temperature during concentration was -100 ° C, and the injection temperature into the column was 100 ° C. The initial column temperature was set at 40 ° C. for 3 minutes, then ramped to 200 ° C. at 20 ° C. every minute for 8 minutes, then maintained at that temperature for 6 minutes and measured. Helium was used as the carrier gas, and the flow rate was 1.71 mL / min.
The peak area was calculated from the obtained chromatograph, and the calculated peak area was defined as the relative concentration of acetone collected from the skin.

(4) Sampling of blood Blood was collected by a doctor from the median vein of each subject or other appropriate site. The blood collection volume was 5 mL each time (1 blood collection tube for blood separation was 5 mL). Serum was separated from the collected blood and subjected to TARC analysis.

(5) Quantification of TARC value The TARC value was quantified from serum obtained by blood collection using a Human CCL17 / TARC Quantikine ELISA Kit (manufactured by R & D systems).

(6) Comparison of Acetone Amount in Skin Gas between Healthy Subjects and Atopic Dermatitis Patients The relative concentration of skin acetone gas calculated by analysis of skin gas was compared between a healthy subject group and an atopic dermatitis patient group (hereinafter, referred to as a group of atopic dermatitis). "AD group"). A Mann-Whitney U test was performed, and those with a p-value less than 0.05 were determined to be significant.
The result is shown in FIG.

As shown in FIG. 2, the relative concentration of acetone in the skin gas was significantly higher in the AD group than in the healthy group.
Thus, a remarkable difference was observed between the healthy person and the atopic dermatitis patient in the relative concentration of acetone in the skin gas.

(7) Calculation of correlation coefficient between relative concentration of acetone in skin gas and TARC value in blood Spearman's rank phase relationship between relative concentration of acetone calculated by analysis of skin gas and TARC value in blood The number was calculated. In addition, the thing whose p value was less than 0.05 was judged as significant.
The result is shown in FIG.

  As shown in FIG. 3, in patients with atopic dermatitis, a significant correlation was observed between the relative concentration of acetone in the skin gas and the blood TARC value known as a severity marker for atopic dermatitis. Was done.

As described above, the amount of acetone contained in the skin gas is measured, and by using the measured amount of acetone as an index, the skin condition related to atopic dermatitis can be evaluated non-invasively, simply and accurately. it can.
Further, by grasping the change of the skin gas component, the change occurring inside the skin can be grasped. Therefore, according to the present invention, it is possible to predict not only the skin condition related to atopic dermatitis presently appearing but also the manifestation of symptoms of atopic dermatitis that may occur in the future.

Claims (10)

  A method for evaluating a skin condition related to atopic dermatitis, wherein a skin condition related to atopic dermatitis of a subject is evaluated using an amount of acetone in a gas volatilized from a skin surface of the subject as an index.   A method for measuring the amount of acetone from a gas volatilized from the skin surface of a subject in order to evaluate a skin condition related to atopic dermatitis.   The method according to claim 1 or 2, wherein gas evolving from the skin surface of the subject is collected, and the amount of acetone contained in the collected gas is measured.   As the skin condition related to atopic dermatitis, the presence or absence of atopic dermatitis, the possibility of the development of atopic dermatitis, the state of prevention of atopic dermatitis, the progress or severity of atopic dermatitis, Topical degree or severity of atopic dermatitis, signs of aggravation of atopic dermatitis, presence or absence of tendency (predisposition) to atopic dermatitis, healing status of atopic dermatitis, or therapeutic effect on atopic dermatitis The method according to any one of claims 1 to 3, wherein the method is evaluated or predicted.   From the skin rash of atopic dermatitis, the rash of atopic dermatitis, the rash adjacent to the rash of atopic dermatitis, or the healthy part of a subject who does not develop atopic dermatitis, The method according to any one of claims 1 to 4, wherein gas volatilized from the gas is collected. Measuring the amount of acetone in the gas volatilized from the skin surface of the subject, measuring means,
Using the measured amount of acetone as an index, to evaluate the skin condition related to the subject's atopic dermatitis, arithmetic means,
An evaluation system for skin conditions related to atopic dermatitis, comprising: A gas sampling means for collecting gas that volatilizes from the skin surface of the subject,
The measuring means measures the amount of acetone contained in the gas collected by the gas collecting means,
The system according to claim 6.   As the skin condition related to atopic dermatitis, the presence or absence of atopic dermatitis, the possibility of the development of atopic dermatitis, the state of prevention of atopic dermatitis, the progress or severity of atopic dermatitis, Topical degree or severity of atopic dermatitis, signs of aggravation of atopic dermatitis, presence or absence of tendency (predisposition) to atopic dermatitis, healing status of atopic dermatitis, or therapeutic effect on atopic dermatitis The system according to claim 6, wherein the system is evaluated or predicted.   From the skin rash of atopic dermatitis, the rash of atopic dermatitis, the rash adjacent to the rash of atopic dermatitis, or the healthy part of a subject who does not develop atopic dermatitis, The system according to any one of claims 6 to 8, wherein gas evaporating from the gas is collected.   A substance which is a candidate for a preventive or ameliorating agent for atopic dermatitis is applied to the skin of a subject, and the method according to any one of claims 1 to 9 is performed, or the atopic dermatitis is used or a system is used. A change in the skin condition of the skin before and after the application of a substance which is a candidate for a preventive or ameliorating agent for atopic dermatitis is confirmed, and a substance having a preventive or ameliorating effect for atopic dermatitis is identified as an agent for preventing or ameliorating atopic dermatitis A method for screening for an agent for preventing or ameliorating atopic dermatitis.

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