JP2019180282A - Plant trait regulator - Google Patents

Abstract

To provide a technique that regulates plant traits including a petal trait.SOLUTION: A polynucleotide is introduced into a plant, the polynucleotide including A) a base sequence encoding a rice-derived specific amino acid sequence, or B) a base sequence encoding an amino acid sequence identical by 90% or more with the amino acid sequence.SELECTED DRAWING: None

Description

  The present invention relates to plant trait regulators and the like.

  The petals are subject to phenotypic control from the viewpoints of improving the appreciation value and the appreciation period. For example, attempts have been made to change the color of petals, change the shape of petals, extend the life of petals, and the like.

  Molecular breeding is often used for such trait regulation. For example, it has been reported that by controlling APETALA3-1 (AP3-1) gene, SEPALLATA (SEP) gene, UNUSUAL FLORAL ORGANS (UFO) gene, PISTILLATA (PI) gene, etc., the trait of Arabidopsis petals was regulated. (Non-Patent Documents 1 to 4). However, when these genes are regulated, there is a problem that the petal changes like a leaf or a leaf and loses the petalness (the petal is thin and the shape is beautiful). Non-Patent Document 5 reports that the EPHEMERAL1 gene controls petal senescence of morning glory.

Yi Y, Jack T. An intragenic suppressor of the Arabidopsis floral organ identity mutant apetala3-1 functions by suppressing defects in splicing.Plant Cell. 1998 10: 1465-1477. Pelaz S, Ditta GS, Baumann E, Wisman E, Yanofsky MF.B and C floral organ identity functions require SEPALLATA MADS-box genes.Nature. 2000 405: 200-203. Queenie E, Tan KG, Hill TA, Irish VF.An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development.Development 2008 135: 1235-1245. Ng M, Yanofsky MF.Activation of the Arabidopsis B class homeotic genes by APETALA1.Plant Cell. 2001 13: 739-753. Kenichi Shibuya, Keiichi Shimizu, Tomoko Niki, Kazuo Ichimura Identification of a NAC transcription factor, EPHEMERAL1, that controls petal senescence in Japanese morning glory.Plant J. (2014) 79, 1044-1051.

  An object of the present invention is to provide a technique for regulating plant traits including petal traits. Preferably, an object of the present invention is to provide a technique for adjusting a petal character while maintaining a petal character (a thin petal, a beautiful shape, etc.). More preferably, an object of the present invention is to provide a technique for regulating at least one petal trait selected from the group consisting of petal life, petal shape, and petal color.

  As a result of diligent research in view of the above problems, the present inventor has found that A) a base sequence encoding the amino acid sequence represented by SEQ ID NO: 1, or B) 90% or more identity with the amino acid sequence represented by SEQ ID NO: 1. By introducing into a plant a polynucleotide comprising a base sequence encoding an amino acid sequence having at least one species selected from the group consisting of plant traits, particularly petal life, petal shape, and petal color, It has been found that adjustments can be made while maintaining the appearance (thinness of petals, beauty of shape, etc.). As a result of further research based on this knowledge, the present inventor completed the present invention.

  That is, the present invention includes the following aspects.

  Item 1. A) containing a polynucleotide comprising a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or B) a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1. , Plant trait regulator.

  Item 2. Item 2. The plant character regulator according to Item 1, wherein the character includes a petal character.

  Item 3. Item 3. The plant trait regulator according to Item 1 or 2, wherein the character includes at least one petal character selected from the group consisting of petal life, petal shape, and petal color.

  Item 4. Item 4. The plant trait regulator according to any one of Items 1 to 3, wherein the character includes at least one petal character selected from the group consisting of petal life and petal shape.

  Item 5. Item 5. The plant trait regulator according to any one of Items 1 to 4, wherein the trait regulation is greening of petals.

  Item 6. Item 6. The plant trait regulator according to any one of Items 1 to 5, wherein the polynucleotide constitutes a vector.

  Item 7. A) containing a polynucleotide comprising a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or B) a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1. A trait-regulated plant or a partial structure thereof containing cells.

  Item 8. A) containing a polynucleotide comprising a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or B) a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1. Seeds that are obtained from or develop into a plant that contains cells and contains a phenotypically regulated organ.

  Item 9. A) a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or B) a polynucleotide comprising a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1 A plant trait regulation method comprising a step of introducing a plant cell.

  Item 10. A) a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or B) a polynucleotide comprising a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1 A method for producing a trait-regulated plant, comprising a step of introducing the plant cell.

  According to the present invention, a technique for regulating plant traits including petal traits can be provided. Preferably, according to the present invention, it is possible to provide a technique for adjusting the petal character while maintaining the petal character (the thinness of the petal, the beauty of the shape, etc.). More preferably, according to the present invention, a technique for regulating at least one petal trait selected from the group consisting of petal life, petal shape, and petal color can be provided.

The observation image of Arabidopsis thaliana in Example 4 is shown. A shows the flower and long-horned fruit in an inflorescence. In A, WT represents wild-type Arabidopsis thaliana and GPP ox represents the Arabidopsis thaliana obtained in Example 1. B shows the oldest flower and its petals in the image of A. In B, the scale bar represents 1 mm. The observation image of the morning glory flower in Example 4 is shown. WT indicates wild-type morning glory, escape (n37-2) indicates a strain that has been introduced with GPP in Example 2 but has become non-transformed by escape, and each of the three GPP ox strains in Example 2. The obtained morning glory is shown. The time-sequential observation image of the morning glory flower in Example 4 is shown. WT represents wild type morning glory, and GPP ox represents morning glory obtained in Example 2. The observation image of the petunia flower obtained in Example 3 in Example 4 is shown.

  In this specification, the expressions “containing” and “including” include the concepts of “containing”, “including”, “consisting essentially of”, and “consisting only of”.

  In the present specification, “identity” of amino acid sequences refers to the degree of amino acid sequence match between two or more comparable amino acid sequences. Therefore, the higher the identity between two amino acid sequences, the higher the identity or similarity of those sequences. The level of amino acid sequence identity is determined, for example, using FASTA, a sequence analysis tool, using default parameters. Alternatively, the algorithm BLAST (KarlinS, Altschul SF. “Methods for assessing the statistical significance of molecular sequence features by using general scoringschemes” Proc Natl Acad Sci USA. 87: 2264-2268 (1990), Karlin S, Altschul SF. "Applications and statistics for multiple high-scoring segments in molecular sequences." Proc Natl Acad Sci USA. 90: 5873-7 (1993)). A program called blastp and a program called tblastn based on the BLAST algorithm have been developed. Specific methods of these analysis methods are known, and the website of the National Center of Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/) may be referred to. The “identity” of the base sequence is also defined according to the above.

  In the present specification, “conservative substitution” means that an amino acid residue is substituted with an amino acid residue having a similar side chain. For example, substitution with amino acid residues having basic side chains such as lysine, arginine, and histidine is a conservative substitution. In addition, amino acid residues having acidic side chains such as aspartic acid and glutamic acid; amino acid residues having non-charged polar side chains such as glycine, asparagine, glutamine, serine, threonine, tyrosine, and cysteine; alanine, valine, leucine, isoleucine, Amino acid residues with non-polar side chains such as proline, phenylalanine, methionine, and tryptophan; Amino acid residues with β-branched side chains such as threonine, valine, and isoleucine; Aromatic side chains such as tyrosine, phenylalanine, tryptophan, and histidine Similarly, substitutions between amino acid residues are conservative substitutions.

1． Plant trait regulator In one aspect of the present invention, A) a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 1 (base sequence A), or B) 90% or more of the amino acid sequence represented by SEQ ID NO: 1 A plant trait regulator (this book) containing a polynucleotide containing a base sequence (base sequence B) encoding an amino acid sequence having identity (sometimes referred to as “polynucleotide of the present invention” in the present specification). In the specification, it may be referred to as “plant trait regulator of the present invention”). This will be described below.

  The protein consisting of the amino acid sequence shown in SEQ ID NO: 1 is an endogenous protein of rice, and it is considered that similar proteins exist in other plants.

  The base sequence A is not particularly limited as long as it encodes the amino acid sequence shown in SEQ ID NO: 1. The base sequence A preferably includes the base sequence represented by SEQ ID NO: 2.

  In the definition of the base sequence B, the identity between the amino acid sequence of the protein encoded by the base sequence B and the amino acid sequence represented by SEQ ID NO: 1 is preferably 95% or more, more preferably 97% or more, and even more preferably 99. % Or more, more preferably 99.5% or more.

One embodiment of the base sequence B includes the following base sequence B1:
B1) A nucleotide sequence (base sequence B1) encoding an amino acid sequence obtained by mutating one or more amino acids with respect to the amino acid sequence shown in SEQ ID NO: 1.

  In the base sequence B1, the plurality is, for example, 2 to 100, preferably 2 to 50, more preferably 2 to 10, and further preferably 2 to 5.

  In the nucleotide sequences B and B1, the mutation of the nucleotide sequence in which the amino acid is mutated is not particularly limited, and examples thereof include substitution, deletion, insertion and addition. Of these, substitution is preferred, and conservative substitution is preferred.

  In the base sequences B and B1, the “amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1” is not particularly limited as long as the protein comprising the sequence does not significantly impair its original function. Typically, the amino acid sequence comprises naturally occurring mutations that are endogenously expressed in the plant plant rice.

  Base sequences B and B1 can preferably hybridize with a complementary strand of base sequence A (preferably SEQ ID NO: 2) under stringent conditions. Stringent conditions are determined by the melting temperature of the nucleic acid that binds the complex or probe, as taught by Berger and Kimmel (1987, Guide to Molecular Cloning Techniques Methods in Enzymology, Vol. 152, Academic Press, San Diego CA). It can be determined based on (Tm). For example, as washing conditions after hybridization, the conditions of about “1 × SSC, 0.1% SDS, 37 ° C.” can be mentioned. It is preferable that the hybridized state be maintained even when washed under such conditions. Although there is no particular limitation, the more stringent hybridization conditions are about “0.5 × SSC, 0.1% SDS, 42 ° C.”, and the more severe hybridization conditions are “0.1 × SSC, 0.1% SDS, 65 ° C.”. Can do.

  In addition to the base sequence A or B, the polynucleotide of the present invention preferably contains a promoter. The promoter is arranged so that the protein encoded by the base sequence A or B can be expressed. The promoter is not particularly limited as long as it can have transcription activity in plant cells. Specific examples of promoters include organ non-specific constitutive promoters such as CaMV35S promoter, UBQ (ubiquitin) promoter and NOS promoter; organ-specific (eg, petal-specific) promoters and the like.

  The polynucleotide of the present invention can contain a nucleotide sequence other than those described above. Other base sequences include, for example, drug resistance genes, reporter protein coding sequences, expression cassettes thereof; protein tag coding sequences; signal sequence coding sequences; replication origins; each element sequence of binary vectors used in the Agrobacterium method ( For example, left boundary area, right boundary area, etc.).

  Examples of drug resistance genes include chloramphenicol resistance gene, tetracycline resistance gene, neomycin resistance gene, erythromycin resistance gene, spectinomycin resistance gene, kanamycin resistance gene, hygromycin resistance gene, puromycin resistance gene and the like.

  The reporter protein is not particularly limited, and examples thereof include a luminescent (colored) protein that emits light (colored) by reacting with a specific substrate, or a fluorescent protein that emits fluorescence by excitation light. Examples of the luminescent (coloring) protein include luciferase, β-galactosidase, chloramphenicol acetyltransferase, β-glucuronidase and the like, and examples of the fluorescent protein include GFP, Azami-Green, ZsGreen, GFP2, HyPer, Sirius, BFP, CFP, Turquoise, Cyan, TFP1, YFP, Venus, ZsYellow, Banana, KusabiraOrange, RFP, DsRed, AsRed, Strawberry, Jred, KillerRed, Cherry, HcRed, mPlum, and the like. The reporter protein includes a fusion protein of a luminescent (chromogenic) protein or fluorescent protein and another protein (eg, seed-specific protein), a known protein tag for the luminescent (chromogenic) protein or fluorescent protein, or a known signal. Proteins to which sequences and the like are added are also included.

  Examples of protein tags include biotin, His tag, FLAG tag, Halo tag, MBP tag, HA tag, Myc tag, V5 tag, and PA tag.

  Examples of the signal sequence include a transcription termination signal. The termination signal is not particularly limited, and examples thereof include a heat shock protein termination signal (HspT: Heat shock protein Terminator), NosT (Noparin synthase Terminator), and 35sT (CaMV35S Terminator).

  The polynucleotide of the present invention may constitute a vector. The type of vector is not particularly limited. For example, plasmid vector; Agrobacterium vector; tobacco mosaic virus, cucumber mosaic virus, African cassava mosaic virus, apple small spherical latent virus, barley spotted mosaic virus, Bean pod mottle virus, Beet curly top virus, Brome mosaic virus, Cabbage leaf curl virus, Cotton leaf crumple virus, Symbidium mosaic virus, Grape A virus, Pea early browning virus, Poplar mosaic virus, Potato X virus, Rice tungro bacilliform virus, Satellite tobacco mosaic virus, Examples include plant virus vectors such as Tobacco curly shoot virus and tobacco stem virus. In addition to the vectors suitable for introduction into plants or plant cells as described above, vectors for transferring the polynucleotide of the present invention to such vectors (for example, gateway (registered trademark) entries). A clone vector etc. can also be mentioned as an example.

  When the polynucleotide of the present invention is introduced into a plant, the base sequence A or B is under the control of an endogenous transcription control region, and / or under the control of the promoter when the polynucleotide of the present invention includes a promoter. The protein encoded by can be expressed.

  The polynucleotide of the present invention can be easily prepared according to a known genetic engineering technique. For example, it can be prepared using PCR, restriction enzyme cleavage, DNA ligation technology, in vitro transcription technology, and the like.

  The form of the plant trait regulator of the present invention is not particularly limited, and may be, for example, a reagent form or a kit form.

  When it is in the form of a reagent, the plant trait regulator of the present invention is not particularly limited as long as it contains the polynucleotide of the present invention, and may further contain other components as necessary. Examples of other components include, but are not limited to, bases, carriers, solvents, dispersants, emulsifiers, buffers, stabilizers, excipients, binders, disintegrants, lubricants, thickeners. Agents, moisturizers, colorants, fragrances, chelating agents and the like.

  When it is in the form of a kit, the plant trait regulator of the present invention is not particularly limited as long as it contains the polynucleotide of the present invention and / or the reagent described above, and the present invention includes a nucleic acid introduction reagent, a buffer solution, etc. as necessary. Other materials, reagents, instruments and the like necessary for carrying out the genome editing method may be appropriately included.

  The “plant” that is the target of trait regulation of the present invention is not particularly limited as long as it is a plant that may have a petal during its growth process. A wide range of plants including, for example, gymnosperms, angiosperm magnolias, monocotyledons, true dicotyledons (roses I, roses II, chrysanthemum I, chrysanthemum II and their outer groups) Can be mentioned. An angiosperm is preferable as the plant. More specific examples of plants include mallow, cruciferous, chrysanthemum, willows, buttercups, camphoraceae, cyperaceae, dolphins, taros, perillas, violets, celerys, boxwoods, azaleas Family, tadaceae, amaranthaceae, convolvulaceae, rose family, sandalwood family, scorpionaceae family, shrimp family, grape family, beech family, honeysuckle family, scorpion family, legume family, mandarin family, scallop family, genus department, saxifrage family, Oleander, Gentianaceae, Fernaceae, Cypressaceae, Cucurbitaceae, Eggplant, Sesameaceae, Psyllidae, Scorpionaceae, Azena, Pleurotusaceae, Calikerae, Scutellaria, Stagidae, Stiridium, Scorpionaceae , Suriana, Muntingia, Kitinaceae, Dipterocarpaceae, Sarcoraenaceae, Algeriaceae, Beninidae Sphaerocepalmaceae, Tetrameles, Begonias, Datiska, Barberry, Lapteridae, Hiblis, Stilbeidae, Scorpaenidae, Heronaceae, Haedokushi, Kiri, Pepperaceae, Didimera, Kinmowalabidae, Tsuruginoshinoda, Examples include plants belonging to the family Tamaidae, Tanabidae, Tsurudaida, Urabosiidae, Shinobuidae, Kinmowalabidae, Camellia pteridae, Tamidaiidae, Nanabaedaidae, Tsurudaidae, Urabosiidae, Shinobuidae, etc.

  In the examples described later, since the plant trait control effect was obtained between species that are separated from each other in the classification of plants (between Arabidopsis thaliana and Asagao petunia), this effect is exhibited regardless of the plant species. Conceivable.

  Moreover, although a limited interpretation is not desired, one end of the mechanism of the present invention is considered as follows. The protein encoded by the base sequence A or B expressed from the polynucleotide of the present invention changes the color of petals by increasing the number of chloroplasts in the cells constituting the petals, and further changes the petal structure to the leaf structure. It is considered that the shape of the petal is changed by approaching to the point, and the structural toughness of the petal is increased to extend its life.

  According to the plant trait modifier of the present invention, various plant traits such as petal traits, more specifically, for example, petal life, petal shape, petal color, and the like can be adjusted. In these more specific examples, the plant trait modifier of the invention can extend the life of the petals, change the shape of the petals, or change the color of the petals (eg greening). ). In this case, the plant trait regulator of the present invention can be used as a petal life extending agent, a petal aging inhibitor, a petal shape modifier, a petal color modifier, a petal greening agent, and the like.

  The plant trait regulator of the present invention can be used as described in “2. Plant trait regulation method, trait-regulated plant or plant cell production method” described later.

2． Plant trait regulation method, method for producing trait-regulated plant The present invention, in one aspect thereof, includes the step of introducing the polynucleotide of the present invention into a plant or plant cell, and a plant trait-regulated plant. (In the present specification, these may be collectively referred to as “the method of the present invention”). These will be described below.

  The plant to be introduced is the same as defined in “1. Plant trait regulator”. The plant cell to be introduced is not particularly limited as long as it is a cell that can develop into a petal, and examples thereof include egg cells. In addition, the introduction site in the plant is not particularly limited, and examples thereof include flowers (especially eggs, pollen, etc. in flowers), leaves, roots, and the like.

  The introduction method is not particularly limited, and can be appropriately selected according to the type of the product to be introduced and the introduction target. Examples of introduction methods include the floral dip method, the floral spray method, the Agrobacterium method, the particle gun method, the infiltration method, the toothpick inoculation method, the suction injection method, the leaf disk method, the inflorescence infiltration method, and the vacuum filtration method. Virus-mediated nucleic acid delivery and the like. Among these, the Agrobacterium method is preferable from the viewpoint of convenience and safety.

  After the introduction, a trait-regulated plant can be obtained by growing the obtained plant or plant cell or by growing from the obtained plant via callus. In addition, after the introduction, cells, tissues and the like into which the polynucleotide of the present invention has been introduced can be selected with a drug as necessary.

  By the method of the present invention, various plants with different degrees of phenotypic regulation can be obtained depending on the expression level of GPP and the like. In this case, for example, a plant having a desired character can be screened from the viewpoint of the degree of petal greening, the petal shape, and the like.

3． Plant, substructure, seed The present invention, in one aspect thereof, is a trait-regulated plant or substructure comprising cells containing the polynucleotide of the present invention, and obtained from or to the plant. Generated seeds.

  The partial structure of the plant is not particularly limited, and examples include grafting scrubs, grafting rootstocks, branches, aerial parts, and the like, and in particular, partial structures that contain or can generate flowers and buds. Is preferred.

  The plant, partial structure, and seed in this section can be obtained according to the above-mentioned “2. Plant trait regulation method, plant-regulated plant or plant cell production method”.

  EXAMPLES The present invention will be described in detail below based on examples, but the present invention is not limited to these examples.

Reference example 1. Preparation of vector containing GPP expression cassette Binary expression cassette comprising base sequence (SEQ ID NO: 2) encoding GPP (Green Petal inducible Protein) protein (amino acid sequence SEQ ID NO: 1) downstream of the cauliflower mosaic virus 35S promoter It was incorporated into a vector (pBIG2113SF) to obtain a binary vector containing a GPP expression cassette.

Example 1. Introduction of GPP to Arabidopsis thaliana
Agrobacterium (Agrobacterium tumefaciens GV3101) containing a GPP expression cassette-containing binary vector (Reference Example 1) was dropped onto Arabidopsis thaliana (ecotype Col-0) flowers to obtain seeds (previously reported: drop method) , Narusaka et al., Plant Biotechnol. 27: 349-351.). The obtained seeds were sown in a medium containing a selection marker (hygromycin) to obtain an Arabidopsis plant into which a GPP expression cassette grown on the medium was introduced.

Example 2 GPP introduction to morning glory (Shimizu et al., (2003), J. Japan. Soc. Hort. Sci. 72: 409-414. And Kikuchi et al., (2005), Plant Biotechnol. 22: 295- 302.), Agrobacterium tumefaciens EHA105 containing a GPP expression cassette-containing binary vector (Reference Example 1) was transformed into Japanese morning glory (Ipomoea nil, the white petal mutant of 'Violet': Ishimaru et al. (1996), J. Plant Physiol. 148: 672-676.)). After introduction, the morning glory with the GPP expression cassette introduced was obtained through callus.

Example 3 Agrobacterium (Agrobacterium tumefaciens) containing a GPP expression cassette-containing binary vector (Reference Example 1) according to the previous report of introduction of GPP into petunia (Jorgensen et al., 1996. Plant Mol. Biol. 31, 957-73) GV3101) was introduced into Petunia × hybrida. After introduction, petunia into which the GPP expression cassette was introduced was obtained through callus.

Example 4 Evaluation of traits The plants obtained in Examples 1 to 3 were observed. An observation image of Arabidopsis thaliana is shown in FIG. 1, an observation image of morning glory is shown in FIGS. 2 and 3, and an observation image of petunia is shown in FIG.

  1-4, it turned out that in the plant in which GPP was introduced, the petals were greened while maintaining the petal-like nature (thinness of petals, beauty of shape, etc.). This indicates that GPP has a function to control petal color (petal greening ability).

  From FIG. 1, it was found that the petals of Arabidopsis introduced with GPP were shorter and rounder than the wild type (data not shown). 2 and 3, it was found that the morning glory petals into which GPP was introduced had a sharper tip (compete bloom) than the wild type. Furthermore, from FIG. 4, petunia petals into which GPP was introduced tended to be slightly smaller and slightly smaller than the wild type (data not shown). From these results, it was found that GPP has a function of changing the shape of petals.

  From FIG. 1, it was found that the Arabidopsis petals into which GPP was introduced did not fall off for a longer time, and maintained the flowering form for a longer time. In addition, it was found from FIG. 3 that the morning glory petals into which GPP was introduced also maintained the shape at the time of flowering for a longer time. From these results, it was found that GPP has a function of extending the life of petals (petal aging suppression function).

Claims (10)

A) a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or
B) A plant trait regulator containing a polynucleotide comprising a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1. The plant trait regulator of Claim 1 in which the said character contains a petal character. The plant trait regulator according to claim 1 or 2, wherein the trait includes at least one petal trait selected from the group consisting of petal life, petal shape, and petal color. The plant trait regulator according to any one of claims 1 to 3, wherein the trait includes at least one petal trait selected from the group consisting of petal life and petal shape. The plant trait regulator in any one of Claims 1-4 whose said trait regulation is greening of a petal. The plant trait regulator in any one of Claims 1-5 in which the said polynucleotide comprises the vector. A) a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or
B) A trait-regulated plant or a partial structure thereof comprising a cell containing a polynucleotide comprising a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1. A) a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or
B) obtained from a plant comprising a cell containing a polynucleotide comprising a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1 and comprising a trait-regulated organ; Alternatively, seeds that develop in the plant. A) a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or
B) A method for regulating plant traits, comprising the step of introducing a polynucleotide comprising a base sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1 into a plant or plant cell. A) a base sequence encoding the amino acid sequence shown in SEQ ID NO: 1, or
B) A method for producing a trait-regulated plant comprising the step of introducing into a plant or plant cell a polynucleotide comprising a nucleotide sequence encoding an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1. .