JP2019162047A - Cell proliferation system in which cell proliferation unit and booth are unified - Google Patents

Abstract

To provide a cell proliferation system having safety and reliability which is approvable as medical equipment.SOLUTION: A cell proliferation system comprises: a cell proliferation unit 10 equipped with an incubator 14 capable of housing a cell culture vessel; and a booth 30 constituting a clean room 35 for arranging the cell proliferation unit 10, in which the booth 30 is equipped with a plurality of sections arranged in series closer to an entrance than is the clean room 35. According to such a constitution, the booth 30 constituting the clean room 35 is provided integrally with the cell proliferation unit 10, so that it is possible to uniformly set a method for preventing invasion of bacteria. Therefore, invasion of bacteria can be prevented according to a predetermined management method, so that a highly reliable system not depending on a use method of a user can be provided.SELECTED DRAWING: Figure 3

Description

  The present invention relates to a cell proliferation system in which a cell proliferation unit and a booth are integrated.

  In recent years, research on regenerative medicine and cell therapy for treating diseases by transplanting adult stem cells has been actively conducted. In order to perform such treatment, methods for culturing and adjusting adult stem cells in tissues collected from patients have been widely studied. By culturing the cells, it is possible to amplify the number of stem cells that are extremely low in the tissue to the number necessary for treatment.

  Such cell culture is performed using a cell culture apparatus equipped with an incubator. And the clean room and facility which arrange | positions such a cell culture apparatus need to be sterilized regularly in order to remove impurities, such as bacteria, and to maintain cleanliness. For example, Patent Document 1 discloses a decontamination apparatus that atomizes a peracetic acid disinfectant by blowing air to discharge droplets. By disposing such a decontamination device in a sealed space, a sterilized booth can be provided.

JP 2014-140481 A

However, in the conventional structure as described above, it is left to the user how to configure the clean room, and there is a problem that a management method such as prevention of invasion of bacteria is not determined. For this reason, there existed a problem that the cell growth system which cleared the manufacturing control and quality control standard (GMP) of a pharmaceutical and a quasi-drug could not be provided. For this reason, there has been a problem that the cell proliferation system is only certified as a physics and chemistry device and cannot be approved as a medical device.
Then, this invention makes it a subject to provide the cell proliferation system which has the safety | security and reliability which can be approved as a medical device.

  The present invention has been made in order to solve the above-described problem, and a cell growth unit including an incubator capable of accommodating a cell culture container, a booth configuring a clean room for arranging the cell growth unit, The booth includes a plurality of compartments arranged in series on the entrance / exit side of the clean room.

  The present invention is as described above, and includes a cell growth unit having an incubator capable of accommodating a cell culture container, and a booth constituting a clean room for arranging the cell growth unit. It has a plurality of sections arranged in series on the side. According to such a configuration, since the booth constituting the clean room is provided integrally with the cell growth unit, it is possible to uniformly set a method for preventing the invasion of bacteria. Therefore, since the invasion of bacteria can be prevented according to a predetermined management method, a highly reliable system that does not depend on the usage method of the user can be provided.

  Further, since a plurality of compartments arranged in series on the entrance / exit side of the clean room are provided, it is possible to perform sterilization and sterilization step by step with these plurality of compartments. That is, when a patient, a doctor, or the like passes through a plurality of sterilized and sterilized compartments, the invasion of bacteria can be more reliably prevented.

  For example, the plurality of compartments include at least an outer chamber disposed at the entrance of the booth, a front chamber disposed adjacent to the outer chamber, and a treatment room disposed between the front chamber and the clean room. You may do it. If comprised in this way, since it must pass at least an outer room, a front room, and a treatment room by the time it reaches a clean room, a clean room can be kept clean. Therefore, impurities such as bacteria are less likely to enter the inside of the cell growth unit, and the risk that such impurities adversely affect cell growth is reduced.

  The booth may be disassembled into a plurality of panels, and a sealed space may be formed by assembling the panels. With this configuration, the booth can be easily installed and disassembled, and the booth size can be easily reduced and expanded. Therefore, even a standardized booth can be easily introduced into various places.

It is a functional block diagram of a cell growth system. It is a block diagram which shows the connection state of a cell growth system. 1 is a schematic view of a sterilization system. It is a front view of the panel which comprises a booth, Comprising: (a) The figure of a standard panel, (b) The figure of a panel with a door, (c) The figure of a panel with an escape part, (d) The figure of a panel with a pass box .

Embodiments of the present invention will be described with reference to the drawings.
The cell proliferation system according to the present embodiment is a system that manages the culture of cells (specimens) collected from a patient, for example. The cultured cells can be transplanted into a patient for cell therapy. This cell proliferation system can be applied to, for example, a system for producing stem cells from blood cells and a system for producing stem cells from other somatic cells. In this embodiment, an example in which the system is used for cell proliferation for therapeutic purposes will be described. However, the present invention is not limited to this, and the present system can be used for culturing any cell.
As shown in FIGS. 1 to 3, the cell growth system includes a cell growth unit 10, a control device 20, a booth 30, and a sterilization device 50.

(Cell proliferation unit 10)
The cell growth unit 10 is a device that receives a sealed container (such as a test tube or a vial) storing somatic cells, automatically executes cell culture, and then discharges the cultured cells in the sealed container. Since the cell proliferation system according to the present embodiment includes a plurality of cell proliferation units 10, it is possible to simultaneously proliferate cells of a plurality of users. The plurality of cell proliferation units 10 are provided in separate housings, and are configured to be separated from each other.

  As shown in FIG. 1, the cell growth unit 10 includes a management unit 11, an operation receiving unit 12, a communication control unit 13, an incubator 14, a temperature adjusting device 15, a temperature sensor 16, a monitor 17, It has. Needless to say, the configuration of the cell growth unit 10 is not limited to this, and may include other configurations.

  The management unit 11 includes a computer and controls the operation of the cell growth unit 10. By providing this management unit 11, each cell proliferation unit 10 includes an independent CPU and an independent operating system. For this reason, each cell proliferation unit 10 is disconnected from the control apparatus 20 mentioned later, and can operate | move stand-alone.

  The operation receiving unit 12 is an input device that receives an operation from a user. The operation receiving unit 12 includes, for example, an input device (one or more) such as a liquid crystal touch panel, a keyboard, a mouse, a button, and a barcode reader. When the operation receiving unit 12 receives an operation from the user, the management unit 11 controls the operation of the cell proliferation unit 10 according to the operation content.

  The communication control unit 13 is for controlling communication with the control device 20 described later. The communication control unit 13 according to the present embodiment is configured to be connected to a network using, for example, Ethernet (registered trademark). In the present embodiment, the cell proliferation unit 10 is connected to a LAN (local area network) using the communication control unit 13. The cell proliferation unit 10 according to the present embodiment is connected to the control device 20 via a LAN so that commands from the control device 20 connected to the same LAN can be received and messages can be transmitted to the control device 20. It is configured. When the communication control unit 13 receives a command transmitted from the control device 20, the cell proliferation unit 10 is controlled based on this command.

  The incubator 14 is an apparatus configured to accommodate a cell culture container in an internal sealed space. In the present embodiment, cells to be cultured are sealed in a cell culture container, and the cell culture container is placed in the incubator 14 to culture the cells. The incubator 14 is provided with a device such as a temperature control device 15 to be described later, so that the temperature and pH of the medium in the cell culture container are controlled, and a state suitable for cell growth is maintained. It has become so.

  In addition, although what is used as a cell culture container is not specifically limited, For example, culture tanks, such as a bottle and a bioreactor, a gas-permeable bag, a flask, a petri dish etc. can be used. Inside the incubator 14 is provided a tray or the like that can hold the cell culture container.

  The temperature adjustment device 15 is a device that automatically performs heating or cooling so as to keep the temperature in the incubator 14 constant. The temperature adjusting device 15 is an electric type, and an output current is controlled in conjunction with a temperature detected by a temperature sensor 16 described later. Thereby, the temperature in the incubator 14 (the temperature detected by the temperature sensor 16) is controlled to be the target temperature.

  The temperature sensor 16 is disposed in the incubator 14 and detects the temperature in the incubator 14. The detection result of the temperature sensor 16 is used for feedback control and error monitoring control of the temperature adjustment device 15. Specifically, the management unit 11 monitors the detection result of the temperature sensor 16, and the temperature in the incubator 14 is kept constant by controlling the output of the temperature adjusting device 15 according to the detection result. It is like that.

  The management unit 11 according to the present embodiment is configured to store the upper limit temperature and the lower limit temperature in the incubator 14 by an administrator setting in advance. And when the temperature which the temperature sensor 16 detected becomes more than upper limit temperature, or when it becomes below lower limit temperature, it is comprised so that temperature abnormality may be detected. As will be described in detail later, when the management unit 11 detects an abnormality in temperature, a warning message notifying the occurrence of an error is created and transmitted to the server 28.

  The monitor 17 is for displaying data relating to the state of the incubator 14 and the cell culture container. The monitor 17 can be composed of a liquid crystal screen, a lamp, and the like, and can display information such as the temperature in the incubator 14, the elapsed time from the start of culture, the number of cells in the cell culture container, and the like. The user can confirm the progress of the culture work by referring to the information displayed on the monitor 17.

(Control device 20)
Next, the control device 20 will be described. The control device 20 is for controlling the cell growth unit 10 described above. The control device 20 includes a CPU independent of the cell growth unit 10 and an operating system independent of the CPU, and is a terminal such as a personal computer in the present embodiment. The control device 20 is connected to a LAN and can control a single or a plurality of cell growth units 10 connected to the same LAN.

  As shown in FIG. 1, the control device 20 includes a registration unit 21, an input unit 22, a control signal output unit 23, a display unit 24, a transmission unit 25, a reception unit 26, and the like when the CPU executes various programs. It functions as each means. Needless to say, the configuration of the control device 20 is not limited to this, and may include other configurations.

  The registration means 21 is for registering the cell proliferation unit 10 to be controlled. The registration unit 21 can register the cell growth unit 10 as a control target of the control device 20 when the user explicitly specifies the cell growth unit 10 connected to the same LAN.

  As a method for registering the cell growth unit 10, the registration means 21 may search for the cell growth unit 10 connected to the LAN. For example, when the user requests a search for the cell growth unit 10, the registration unit 21 may search the LAN and list the cell growth unit 10. Then, by selecting the cell growth unit 10 to be registered from the cell growth units 10 listed as the search result, the cell growth unit 10 can be registered as a control target of the control device 20. Good.

  In this embodiment, as shown in FIG. 2, by registering a plurality of cell growth units 10 in the control device 20, the plurality of cell growth units 10 are connected to the control device 20 in an array to form one system. It can be operated. The cell proliferation unit 10 registered in the control device 20 is given a unique identifier in the system at the time of registration. Thereby, each cell proliferation unit 10 in the system is identified by this identifier.

  The input unit 22 is an input device such as a liquid crystal touch panel, a keyboard, and a mouse connected to the terminal. This input means 22 is used by an administrator who operates the control device 20. For example, it is used for inputting a command to be transmitted to the cell proliferation unit 10 by the administrator.

  The control signal output means 23 is for transmitting a command to the cell proliferation unit 10 connected to the same LAN. That is, when a command is input by the administrator using the input means 22 described above, the command is transmitted to the cell growth unit 10. For example, when the administrator designates a specific cell growth unit 10 and inputs a command to stop the culture operation, the command is transmitted to the specific cell growth unit 10 by the control signal output means 23. And the specific cell growth unit 10 which received the command will perform the operation | movement (stop of culture | cultivation work) according to the said command.

  The display means 24 is for displaying a video signal output from the CPU of the control device 20. In this embodiment, it is a display of a terminal. The administrator can monitor the operation of the cell growth unit 10 and input a control command while confirming the contents displayed on the display means 24.

  The transmission means 25 is for transmitting a message via the Internet. For example, when an error occurs, the transmission unit 25 transmits a message to the predetermined server 28 registered in advance via the Internet.

  The receiving means 26 is for receiving a message via the Internet. For example, the receiving unit 26 receives a message from a predetermined server 28 registered in advance, and executes control according to the received message.

(Cell proliferation procedure)
Cell proliferation using the cell proliferation system according to the present embodiment is executed in the following procedure.
First, a tissue is collected from a patient by a doctor, thereby obtaining cells to be cultured. The cells are stored in a sealed container such as a bottle.

  Then, the person in charge of the culture operation activates the cell growth unit 10 and inputs a command to start culture. At this time, the operator may input parameters necessary for culture. For example, a patient identification number, information (volume) on the cell culture container, and the like may be input. In order to facilitate the input of these parameters, a barcode may be attached to a sealed container or a cell culture container. By reading this barcode with a barcode reader provided in the cell growth unit 10, parameters can be easily input.

  When the cell growth unit 10 receives the culture start command, the management unit 11 checks the available space in the incubator 14. When it is determined that there is sufficient space, a pass box for delivering a sealed container or a cell culture container to the cell growth unit 10 is activated. The worker in charge puts the sealed container and the cell culture container into the pass box. The cell growth unit 10 automatically pulls back the pass box, thereby taking the sealed container and the cell culture container into the cell growth unit 10. The sealed container and the cell culture container taken into the inside are appropriately processed by the robot arm, and the culture is started.

  At this time, the cell proliferation unit 10 may notify the worker in charge of replenishment of various consumables. For example, the operator may be notified to put a container filled with a culture medium, a container filled with a growth factor, and the like into the pass box. And you may make it wait for the start of culture | cultivation until a required consumable is provided.

  When the necessary preparation is completed, the cell growth unit 10 places the cell culture container in the incubator 14. Then, fluid and cells are supplied to the cell culture container. Specifically, a predetermined amount of culture medium or growth factor is supplied to the cell culture container via a tube. Thereafter, the cells contained in the sealed container are supplied to the cell culture container.

  The cell culture container supplied with cells in this way is stored in the incubator 14 until a set culture period (for example, several days) elapses. In the incubator 14, cell growth proceeds by maintaining the cell culture container at a constant temperature.

When the culture period has elapsed and the cells necessary for treatment are ready, the cell growth unit 10 notifies the operator in charge of the completion of the culture. When the worker who receives the notification requests the cell growth unit 10 to receive cells, the cell growth unit 10 takes out the cell culture container from the incubator 14 and loads the cells in the cell culture container into the syringe. This syringe is discharged to the outside of the cell growth unit 10 through a pass box.
The cells thus collected are used by being injected into a patient's body, for example, for cell therapy.

  In the present embodiment, since there are a plurality of cell growth units 10, separate cell cultures may be performed in the plurality of cell growth units 10. Further, a plurality of cell culture containers may be accommodated in the incubator 14, and separate cell cultures may be performed in these cell culture containers. The separate cells may be collected from the same patient or may be collected from different patients.

(About network configuration)
As shown in FIG. 2, the control device 20 and the cell proliferation unit 10 are connected to each other using a LAN. It does not matter whether the LAN connection method is wired connection or wireless connection. Further, the communication between the control device 20 and the cell growth unit 10 is encrypted and protected from unauthorized access.

  Each cell growth unit 10 is connected to a common control device 20. The control device 20 can output a control command to each cell growth unit 10 and can receive a response from each cell growth unit 10. For this reason, all the cell growth units 10 in the system can be centrally managed by one control device 20.

  On the other hand, the control device 20 and each cell proliferation unit 10 are each provided with an independent CPU and an independent operating system. Therefore, the cell growth unit 10 can continue to operate as a stand-alone machine even when it is disconnected from the control device 20. For example, even if a failure occurs in the network connecting the control device 20 and each cell growth unit 10, the cell growth unit 10 can be disconnected from the control device 20 and continue to operate as a stand-alone machine. When the network connection is restored, the cell proliferation unit 10 is automatically reconnected to the system, and the control of the cell proliferation unit 10 by the control device 20 is resumed.

  Note that the cell proliferation unit 10 to be controlled by the control device 20 is limited to the cell proliferation unit 10 registered by the registration means 21. For this reason, it is also possible to provide a cell growth unit 10 that is disconnected from the control device 20 and operates stand-alone despite being arranged in the same LAN as the cell growth unit 10 shown in FIG. It is.

  Further, the registration means 21 can not only register the cell proliferation unit 10 but also cancel the registration of the cell proliferation unit 10. For this reason, even if the cell growth unit 10 has already been registered by the registration means 21, the cell growth unit 10 can be controlled stand-alone by explicitly canceling the registration from the registration means 21.

  If the control device 20 and the cell proliferation unit 10 constituting these systems are connected by a LAN, no other physical connection is required. For this reason, the cell growth unit 10 can be arranged in different rooms on the same floor, different floors of the same building, or can be arranged in different rooms of different buildings. Moreover, the control apparatus 20 can be arrange | positioned adjacent to any cell growth unit 10 in a system, and can also be arrange | positioned in the position away from the cell growth unit 10 using LAN.

(About server 28)
The system according to the present embodiment can remotely manage the system via the Internet by registering the server 28 outside the LAN in the control device 20. In addition, as the server 28 registered in the control device 20, for example, a server of a company that provides the system, a user terminal, or the like can be considered.
Specifically, the control device 20 and the cell proliferation unit 10 according to the present embodiment are connected to the Internet.

  In addition, the control device 20 according to the present embodiment includes a transmission unit 25 that transmits the state of the cell growth unit 10 via the Internet. The transmission means 25 can transmit a message to the server 28 using e-mail or a dedicated application, for example. By using this transmission means 25, for example, the status of cell culture can be periodically reported, or a warning message can be transmitted when an error occurs in any of the cell growth units 10.

  The error of the cell growth unit 10 occurs when, for example, the control device 20 detects a temperature abnormality in the incubator 14. When the cell growth unit 10 finds an abnormality in the state of the incubator 14 or the cell culture container, the cell growth unit 10 creates a warning message including information (such as the cell culture container ID and the cell provider ID) that can identify the cell culture container. Then, a message is transmitted to the server 28 via the control device 20. When the server 28 receives the message, an external person can be notified of the occurrence of the error. In addition, when an error occurs in this way, the cell proliferation unit 10 may automatically execute a process of interrupting the culture process.

  The server 28 may be able to control the cell growth unit 10 via the Internet. For example, from the authenticated server 28, a predetermined command such as stopping the cell growth unit 10 may be transmitted. Alternatively, a predetermined command such as stopping the cell growth unit 10 may be transmitted only when an error occurs. The command transmitted from the server 28 is received by the receiving means 26 of the control device 20 and transmitted to the cell proliferation unit 10. Thereby, you may enable it to operate the cell growth unit 10 remotely.

  For example, when an error occurs and the cell growth unit 10 automatically interrupts the culture process, a command for executing cell culture re-execution may be transmitted from the server 28. Then, the cell proliferation unit 10 that has received a command for performing cell culture re-execution may be configured to automatically replace the culture medium and cells and perform cell culture re-execution. In this way, even when an error occurs and the culture process is interrupted, the culture process can be resumed early by remote operation.

  In this embodiment, the control device 20 includes the transmission unit 25 and the reception unit 26. However, the present invention is not limited thereto, and the cell growth unit 10 may include the transmission unit 25 and the reception unit 26. Good. That is, the cell proliferation unit 10 may be able to communicate with the server 28 via the Internet without using the control device 20.

(About booth 30)
The cell proliferation unit 10 according to this embodiment is arranged in a booth 30 as shown in FIG. The booth 30 includes a plurality of panels 36 having the same size, and the wall surface, the floor surface, and the ceiling surface are configured by the panels 36. That is, the booth 30 can be disassembled into a plurality of panels 36, and a sealed space can be formed by assembling the plurality of panels 36.

  The interior of the booth 30 is divided into several sections, and a clean room 35 for arranging the cell growth unit 10 is configured by one of the sections. Further, the other compartments in the booth 30 are adjacent to the outer chamber 31 disposed at the entrance of the booth 30, the first front chamber 32 disposed adjacent to the outer chamber 31, and the first front chamber 32. The 2nd front chamber 33 arrange | positioned and the treatment room 34 arrange | positioned adjacent to the 2nd front chamber 33 are comprised. The outer chamber 31, the first front chamber 32, the second front chamber 33, and the treatment room 34 are used as a front chamber of the clean room 35, and are arranged in series on the entrance / exit side of the clean room 35. Yes. The outside of the booth 30 and the outer chamber 31 can enter and exit through the door 37, and the outer chamber 31 and the first front chamber 32 can enter and exit through the door 37. 2 The front chamber 33 can be accessed by the door 37, the second front chamber 33 and the treatment room 34 can be accessed by the door 37, and the treatment room 34 and the clean room 35 can be accessed by the door 37. It has become. For this reason, in order from the entrance / exit side, the entrance and exit to the treatment room 34 and the clean room 35 must be made without passing through the outer room 31 → the first front room 32 → the second front room 33 → the treatment room 34 → the clean room 35 It has become.

  The first front chamber 32 and the second front chamber 33 described above are provided to prevent bacteria from entering the treatment room 34 and the clean room 35. In other words, it is configured so that the patient and the operator are surely sterilized by passing through the first front chamber 32 and the second front chamber 33 and do not bring bacteria into the treatment room 34 and the clean room 35.

  The treatment room 34 is a work place for collecting tissue from a patient. In the treatment room 34, surgical equipment may be arranged. Here, the cells collected from the patient are stored in a sealed container such as a bottle. This sealed container is delivered to the clean room 35 via a pass box 38 provided between the treatment room 34 and the clean room 35. With this configuration, the sealed container can be delivered to the worker in the clean room 35 without the patient or doctor entering the clean room 35. The worker in the clean room 35 puts the received sealed container into the cell growth unit 10 according to the procedure already described, and starts cell culture.

  Here, the first front chamber 32, the second front chamber 33, the treatment room 34, and the clean room 35 are each provided with a supply port 39 for introducing ozone gas generated by the discharge device 52 described later. ing.

  Further, in each of the first front chamber 32, the second front chamber 33, the treatment room 34, and the clean room 35, sensors (not shown) for detecting the concentration of ozone gas in the booth 30 are arranged. Note that only one sensor may be disposed in each section, or a plurality of sensors may be disposed in one section. The concentration of ozone gas detected by this sensor is acquired by a sterilizer 50 described later and used for feedback control.

(About the sterilizer 50)
The sterilizer 50 is a device that executes sterilization / sterilization processing in the booth 30. As shown in FIG. 3, the sterilizer 50 includes a PSA oxygen generator 51 and a discharge device 52.

  The PSA oxygen generator 51 is a device that separates air in the atmosphere into nitrogen and high-concentration oxygen, and a known device can be used. The oxygen generated by the PSA oxygen generator 51 is sent to the discharge device 52 for use.

  The discharge device 52 is a device that generates ozone by applying a voltage between electrodes to generate discharge, and a known device can be used. The discharge device 52 includes an air channel to which oxygen generated by the PSA oxygen generator 51 is supplied, generates discharge plasma on the air channel, and passes through the air channel. It is configured to generate ozone from.

  The ozone gas generated by the discharge device 52 is supplied into the booth 30 through a pipe. The pipe for supplying ozone gas connects the sterilizer 50 and the supply port 39. For this reason, when the discharge device 52 generates ozone gas, the ozone gas is ejected from the supply port 39 into the booth 30. Thus, by supplying ozone gas into the booth 30 and filling the inside of the booth 30 with ozone gas, sterilization and sterilization in the booth 30 are performed.

  In the sterilization apparatus 50, a specified value of a desirable ozone gas concentration is set in advance by an administrator or the like. The sterilizer 50 adjusts so that the ozone gas concentration in each compartment is maintained at a value close to this specified value. Note that this specified value may be set for each section. If it can be set for each section, sterilization and sterilization can be performed at different levels for each section.

  As described above, the first anterior chamber 32, the second anterior chamber 33, the treatment room 34, and the clean room 35 are provided with sensors for detecting the concentration of ozone gas in the booth 30. And these sensors are connected to the sterilizer 50, and the sterilizer 50 is comprised so that the density | concentration of the ozone gas of each division can be detected.

When the sensor detects that the concentration of ozone gas has fallen below a specified value in any of the compartments provided with these sensors, the sterilizer 50 starts generating ozone gas and supplies the ozone gas to the compartment. . Then, when the sensor detects that the ozone gas concentration exceeds the specified value in the section due to such supply of ozone gas, the sterilizer 50 stops generating ozone gas.
By such processing, each section is configured so that the concentration of ozone gas is automatically kept constant.

(About panel 36)
The panels 36 constituting the booth 30 are basically formed in the same size. The size of the panel 36 according to the present embodiment is set to, for example, 800 mm wide × 2000 mm long. In the example of FIG. 3, five panels 36 are used for all the wall surfaces, and the installation area is 4 m × 4 m (each side is 800 mm × 5 = 4 m). The height of the booth 30 is 2 m.

  Although not specifically shown, panels 36 having the same size are also arranged on the ceiling surface and the floor surface. In the present embodiment, ten panels 36 are used for the ceiling surface and the floor surface, respectively. These ten panels 36 are fixed in a state where two in the longitudinal direction and five in the lateral direction are arranged.

  In the present embodiment, the panels 36 are all formed in the same size, but the present invention is not limited to this, and the panel 36 having a half size (800 mm wide × 1000 mm long) may be provided. .

There are a plurality of types of panels 36 having different functions. For example, as shown in FIG. 4, a standard panel 41, a panel 42 with a door, a panel 43 with an escape portion, and a panel 44 with a pass box are prepared.
Of these, the standard panel 41 is simply a flat panel 36.
The door-attached panel 42 is a panel 36 including a door 37. This panel 42 with a door is installed in the place where the door 37 is to be provided.

  The panel 43 with an escape part is the panel 36 provided with the escape part 43a which can be broken through in an emergency. Since the escape portion 43a is formed more fragile than the frame of the panel 43 with the escape portion, the escape portion 43a can be easily penetrated to form an escape outlet by applying a force to the escape portion 43a. This panel 43 with an escape part is installed in the wall surface which faces the exterior of the booth 30. FIG. In addition, you may provide one panel 43 with this escape part in each division.

  The panel 44 with a pass box is a panel 36 having a pass box 38. The pass box 38 is for delivering a container such as a sealed container in which cells are enclosed, and is provided so as to protrude from the surface of the panel 36. The pass box 38 has windows on both the delivery side and the receiving side, and is configured so that the two windows cannot be opened simultaneously. Therefore, the delivery side and the receiving side are configured not to communicate with each other. This panel 44 with a pass box is used for the wall between the treatment room 34 and the clean room 35.

(Summary)
As described above, according to the present embodiment, the cell growth unit 10 including the incubator 14 that can accommodate the cell culture container, and the booth 30 that constitutes the clean room 35 in which the cell growth unit 10 is arranged. The booth 30 includes a plurality of compartments arranged in series on the entrance / exit side of the clean room 35. According to such a configuration, since the booth 30 configuring the clean room 35 is provided integrally with the cell growth unit 10, it is possible to uniformly set a method for preventing the invasion of bacteria. Therefore, since the invasion of bacteria can be prevented according to a predetermined management method, a highly reliable system that does not depend on the usage method of the user can be provided.

  In addition, since a plurality of compartments arranged in series on the entrance / exit side of the clean room 35 are provided, it is possible to perform sterilization and sterilization step by step with the plurality of compartments. That is, when a patient, a doctor, or the like passes through a plurality of sterilized and sterilized compartments, the invasion of bacteria can be more reliably prevented.

  Specifically, as a plurality of compartments, at least the outer chamber 31 disposed at the entrance of the booth 30, the front chambers 32 and 33 disposed adjacent to the outer chamber 31, the front chambers 32 and 33, and the clean room 35 And a treatment room 34 disposed between the two. If comprised in this way, since it must pass at least the outer room 31, the front rooms 32 and 33, and the treatment room 34 by the time it reaches the clean room 35, the clean room 35 can be kept clean. Therefore, since impurities such as bacteria hardly enter the inside of the cell growth unit 10, the risk that such impurities adversely affect cell growth is reduced.

  Further, the booth 30 can be disassembled into a plurality of panels 36, and the sealed space can be formed by assembling the panels 36. If comprised in this way, the work of installation and disassembly of the booth 30 is easy, and the size reduction and expansion of the booth 30 are also easy. Therefore, even the standardized booth 30 can be easily introduced into various places.

DESCRIPTION OF SYMBOLS 10 Cell proliferation unit 11 Management part 12 Operation reception part 13 Communication control part 14 Incubator 15 Temperature control apparatus 16 Temperature sensor 17 Monitor 20 Control apparatus 21 Registration means 22 Input means 23 Control signal output means 24 Display means 25 Transmission means 26 Reception means 28 Server 30 Booth 31 Outer room 32 First front room 33 Second front room 34 Treatment room 35 Clean room 36 Panel 37 Door 38 Pass box 39 Supply port 41 Standard panel 42 Panel with door 43 Panel with escape part 43a Exit part 44 With pass box Panel 50 Sterilizer 51 PSA oxygen generator 52 Discharge device

Claims (3)

A cell growth unit equipped with an incubator capable of accommodating a cell culture vessel;
A booth constituting a clean room for arranging the cell proliferation unit;
With
The cell proliferation system, wherein the booth includes a plurality of compartments arranged in series on the entrance / exit side of the clean room.   As the plurality of compartments, at least an outer chamber disposed at the entrance of the booth, a front chamber disposed adjacent to the outer chamber, and a treatment room disposed between the front chamber and the clean room, The cell proliferation system according to claim 1, further comprising:   The cell proliferation system according to claim 1 or 2, wherein the booth can be disassembled into a plurality of panels, and a sealed space can be formed by assembling the panels.

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