JP2019150005A - Salty taste enhancer - Google Patents

Salty taste enhancer Download PDF

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JP2019150005A
JP2019150005A JP2018050164A JP2018050164A JP2019150005A JP 2019150005 A JP2019150005 A JP 2019150005A JP 2018050164 A JP2018050164 A JP 2018050164A JP 2018050164 A JP2018050164 A JP 2018050164A JP 2019150005 A JP2019150005 A JP 2019150005A
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piperine
salty taste
salt
limonene
salty
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JP7159526B2 (en
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本間 亮介
Ryosuke Honma
亮介 本間
中村 直樹
Naoki Nakamura
直樹 中村
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Ikeda Shokken KK
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract

To provide: a salty taste enhancer that has a salty taste enhancing effect, is inexpensive and has high versatility as a material that can make feel salty taste more than the salt content amount and can substantially decrease salt; and, furthermore, a food/drink containing a salty taste enhancer and a substitute salt for reducing salt.SOLUTION: The inventors have found that an emulsion containing piperine has a salty taste enhancing effect and completed the present invention.SELECTED DRAWING: None

Description

本発明は、塩味増強剤及びその製造方法等に関する。  The present invention relates to a salty taste enhancer and a method for producing the same.

食塩(塩化ナトリウム)は、塩味を付与する調味料として種々の飲食物に用いられているが、高血圧症等の関係上、摂取量の低減が求められている。一方で、食塩を減らした場合、塩味が薄くなることで食事の美味しさが損なわれ、味付けとして満足感が得にくいため、実質的に食塩を減らしつつ、塩味は落とさない素材が求められていた。  Sodium chloride (sodium chloride) is used in various foods and drinks as a seasoning for imparting a salty taste. However, in view of hypertension and the like, reduction of intake is required. On the other hand, when salt is reduced, the deliciousness of the meal is impaired due to thin saltiness, and it is difficult to obtain satisfaction as a seasoning. Therefore, there has been a demand for a material that does not substantially reduce salt while reducing salt. .

これまでに各種素材による塩味増強剤が開発されており、例えば、コショウ、ショウガ、クローブ及びシナモンの各香辛料抽出物の混合物を特定の割合で含有することを特徴とする塩味増強剤(特許文献1)や、サンショウの超臨界又は亜臨界二酸化炭素抽出物及び溶媒抽出物から選ばれるサンショウ抽出物並びにフタライド類を有効成分として含有する塩味含有飲食品の塩味増強剤(特許文献2)等が知られている。  So far, salty taste enhancers based on various materials have been developed. For example, salty taste enhancers characterized by containing a mixture of spice extracts of pepper, ginger, clove and cinnamon at a specific ratio (Patent Document 1) ), And a salty taste enhancer for salty foods and beverages containing as an active ingredient a salamander extract selected from supercritical or subcritical carbon dioxide extracts and solvent extracts of salamates (Patent Document 2), etc. Are known.

特許第5872187号公報Japanese Patent No. 5872187 特許第5733737号公報Japanese Patent No. 5733737

本発明は、食塩含有量以上に塩味を感じさせることができ、実質的に食塩を減らすことができる素材として、塩味増強効果を有し、安価で汎用性の高い、塩味増強剤を提供するものである。さらに、塩味増強剤を含む飲食物を提供し、減塩用代用塩を提供する。  The present invention provides a salty taste enhancer that has a salty taste enhancing effect, is inexpensive and highly versatile, as a material that can make salty taste more than the salt content and can substantially reduce salt. It is. Furthermore, food and drink containing a salty taste enhancer is provided, and a salt substitute for reducing salt is provided.

発明者らは、ピペリンを含む乳化物が塩味増強効果を有することを見出し、本発明を完成した。  The inventors have found that an emulsion containing piperine has a salty taste enhancing effect and completed the present invention.

すなわち、本発明は、以下の[1]〜[7]の態様に関する。
[1]ピペリンを含む乳化物を有効成分とする、塩味増強剤。
[2]さらにリモネンを含有する、[1]記載の塩味増強剤。
[3]水溶性高分子を含有する、[1]又は[2]に記載の塩味増強剤。
[4]食塩1gに対し、ピペリンを0.5μg〜200μg用いることを特徴とする、[1]〜[3]の何れかに記載の塩味増強剤。
[5]ピペリン100重量部に対して、リモネン0.3〜100重量部を含む、[1]〜[4]の何れかに記載の塩味増強剤。
[6][1]〜[5]の何れかに記載の塩味増強剤を含む、飲食物。
[7]塩化ナトリウムと[1]〜[5]の何れかに記載の塩味増強剤とを含む、減塩用代用塩。
That is, the present invention relates to the following aspects [1] to [7].
[1] A salty taste enhancer comprising an emulsion containing piperine as an active ingredient.
[2] The salty taste enhancer according to [1], further containing limonene.
[3] The salty taste enhancer according to [1] or [2], which contains a water-soluble polymer.
[4] The salty taste enhancer according to any one of [1] to [3], wherein 0.5 μg to 200 μg of piperine is used per 1 g of sodium chloride.
[5] The salty taste enhancer according to any one of [1] to [4], which contains 0.3 to 100 parts by weight of limonene with respect to 100 parts by weight of piperine.
[6] A food or drink comprising the salty taste enhancer according to any one of [1] to [5].
[7] A salt substitute for reducing salt, comprising sodium chloride and the salty taste enhancer according to any one of [1] to [5].

本発明の塩味増強剤は、塩味増強効果を有し、食塩含有量以上の塩味を感じさせることができるため、実質的に食塩を減らしつつ、飲食物の塩味は落とさない素材として、飲食物中の食塩を減らすことが可能になった。また、安価で汎用性が高く、安全で、さらに味や風味への影響がほとんどないため、広く飲食物への利用が可能である。さらに該塩味増強剤を用いることで、安く、簡便に減塩用代用塩を製造することができる。該塩味増強剤及び減塩用代用塩を用いることで、飲食物中の実質的な食塩量を減らしつつ、塩味は落とさないため、食事の美味しさが損なわれることなく、減塩しても満足感が得られる味付けが可能になった。  The salty taste enhancer of the present invention has a salty taste enhancing effect and can make the salty taste more than the salt content, so that the salty taste of food and drink is not dropped while substantially reducing salt, It became possible to reduce salt. In addition, it is inexpensive, highly versatile, safe, and has little influence on taste and flavor, so it can be widely used for food and drink. Furthermore, by using the salty taste enhancer, a salt substitute for reducing salt can be produced inexpensively and easily. By using the salty taste enhancer and salt substitute for reducing salt, the saltiness is not lost while reducing the amount of salt in foods and drinks. Seasoning that gives you a feeling is now possible.

本発明の塩味増強剤は、ピペリンを含む乳化物を有効成分として含有する。本発明に用いるピペリンは、特に限定されないが、コショウ(Piper nigrum)の果実から抽出することにより得られ、例えばヒハツやヒハツモドキ等のピペリンを含む他の植物から抽出してもよい。抽出は、ピペリンが抽出できれば特に限定されず、常法により抽出することができ、精製してもよく、ピペリンを含有する市販の抽出物を利用できる。  The salty taste enhancer of the present invention contains an emulsion containing piperine as an active ingredient. Although piperine used for this invention is not specifically limited, It may be obtained by extracting from the fruit of pepper (Piper nigrum), for example, you may extract from other plants containing piperine, such as a japonicus and a tiger beetle. Extraction is not particularly limited as long as piperine can be extracted, and it can be extracted by a conventional method, purified, or a commercially available extract containing piperine can be used.

本発明の塩味増強剤は、ピペリンにさらにリモネンを併用することで塩味増強効果が高められる。本発明に用いるリモネンは、特に限定されないが、柑橘系の果皮や果汁から常法により抽出することができ、精製してもよいが、合成品でもよく、d−リモネンを含有する市販の香料、精油、果皮抽出物等を利用できる。  The salty taste enhancer of the present invention can enhance the salty taste enhancing effect by further using limonene in combination with piperine. Limonene used in the present invention is not particularly limited, but it can be extracted from citrus peel and fruit juice by a conventional method and may be purified, but may be a synthetic product, a commercially available fragrance containing d-limonene, Essential oil, peel extract, etc. can be used.

リモネンはピペリンとの併用で塩味増強効果を示せれば特に限定されないが、ピペリン100重量部に対する添加量として、リモネン0.3〜100重量部が好ましく、0.5〜90重量部がより好ましく、1.0〜80重量部がさらに好ましい。  Limonene is not particularly limited as long as it shows a salty taste enhancing effect in combination with piperine, but the amount added to 100 parts by weight of piperine is preferably 0.3 to 100 parts by weight, more preferably 0.5 to 90 parts by weight. More preferably, 0 to 80 parts by weight.

ピペリンを含む乳化物は、塩味増強効果を有していれば特に限定されないが、ピペリン、油脂及び乳化剤を乳化処理した乳化物であるのが好ましい。また、さらにリモネンを併用する場合は、ピペリンを含む乳化物にリモネンを含む乳化物を併用してもよく、ピペリン及びリモネンを乳化した乳化物を使用してもよい。リモネンを含む乳化物は、例えばリモネン、油脂及び乳化剤を乳化処理して得られる。  The emulsion containing piperine is not particularly limited as long as it has a salty taste enhancing effect, but is preferably an emulsion obtained by emulsifying piperine, fats and oils and an emulsifier. Further, when limonene is used in combination, an emulsion containing limonene may be used in combination with an emulsion containing piperine, or an emulsion obtained by emulsifying piperine and limonene may be used. The emulsion containing limonene is obtained, for example, by emulsifying limonene, fats and oils and an emulsifier.

乳化処理に用いる油脂は、食用であれば特に限定されず、大豆油、ゴマ油、落花生油、トウモロコシ油、菜種油、米油、ヤシ油、パーム油等の植物性油脂、豚脂、鶏油、牛脂、魚油、バター等の動物性油脂、硬化油等が例示でき、二種類以上の油脂を組み合わせて用いてもよい。  The fats and oils used for the emulsification treatment are not particularly limited as long as they are edible. Vegetable oils such as soybean oil, sesame oil, peanut oil, corn oil, rapeseed oil, rice oil, palm oil, palm oil, pork fat, chicken oil, beef fat Animal oils and fats such as fish oil and butter, hydrogenated oils and the like can be exemplified, and two or more types of oils and fats may be used in combination.

乳化処理に用いる乳化剤は、乳化作用を有していれば特に限定されないが、水溶性高分子が好ましく、平均分子量1万以上の水溶性高分子がより好ましく、平均分子量5万以上がさらに好ましく、例えば、アラビアガム、オクテニルコハク酸澱粉ナトリウム等の多糖類等が例示でき、2種類以上の乳化剤を組み合わせて用いてもよい。  The emulsifier used in the emulsification treatment is not particularly limited as long as it has an emulsifying action, but is preferably a water-soluble polymer, more preferably a water-soluble polymer having an average molecular weight of 10,000 or more, and further preferably an average molecular weight of 50,000 or more, Examples thereof include polysaccharides such as gum arabic and sodium starch octenyl succinate, and two or more emulsifiers may be used in combination.

乳化処理は、一般的な乳化方法で行うことができ、高圧ホモジナイザー、コロイドミル、超音波乳化機、ホモミキサー、ホモディスパー等の乳化装置を例示でき、二種類以上の装置を組み合わせてもよい。また、乳化処理時に流動性があれば特に限定されないが、乳化処理前に加熱工程を含むのが好ましく、例えば40〜100℃、50〜80℃等の加熱が例示できる。  The emulsification treatment can be performed by a general emulsification method, and examples thereof include an emulsification apparatus such as a high-pressure homogenizer, a colloid mill, an ultrasonic emulsifier, a homomixer, and a homodisper, and two or more kinds of apparatuses may be combined. Moreover, it will not specifically limit if there exists fluidity at the time of an emulsification process, However, It is preferable to include a heating process before an emulsification process, for example, heating of 40-100 degreeC, 50-80 degreeC etc. can be illustrated.

本発明の塩味増強剤は、塩味を有する各種飲食物に添加して使用することができる。飲食物は特に制限されないが、例えばハンバーグ、シチュー等の調理加工食品類、醤油、味噌、だし、ソース等の調味料、昆布茶、トマトジュース、野菜ジュース等の飲料等が例示できる。飲食物への該塩味増強剤の添加量としては、塩味増強効果を示せれば特に限定されないが、飲食物中の食塩1gに対し、ピペリンを0.5μg〜200μg用いるのが好ましく、より好ましくは1.0μg〜150μg、さらに好ましくは2.0μg〜100μg用いるのが好ましい。各種飲食物に添加することで、食塩含有量以上の塩味を感じることができるため、本来の食塩濃度を100%とした場合に、100%以上の塩味を感じることができ、好ましくは105%以上、より好ましくは110%以上の塩味を感じることができることで、飲食物中の食塩を減らすことが可能になる。  The salty taste enhancer of the present invention can be used by adding to various foods and drinks having a salty taste. The food and drink are not particularly limited, and examples thereof include cooked and processed foods such as hamburger stew, seasonings such as soy sauce, miso, dashi, and sauce, and beverages such as kelp tea, tomato juice, and vegetable juice. The amount of the salty taste enhancer added to foods and drinks is not particularly limited as long as it shows a salty taste enhancing effect, but it is preferable to use 0.5 μg to 200 μg of piperine with respect to 1 g of salt in foods and drinks, and more preferably 1 It is preferable to use 0.0 μg to 150 μg, more preferably 2.0 μg to 100 μg. By adding it to various foods and drinks, it is possible to feel a salty taste that exceeds the salt content, so that when the original salt concentration is 100%, a salty taste of 100% or more can be felt, preferably 105% or more. More preferably, the saltiness of 110% or more can be felt, so that salt in food and drink can be reduced.

また、食塩と該塩味増強剤とを混合することで、減塩用代用塩を製造することができる。食塩1gに対し、ピペリンを0.5μg〜200μg用いるのが好ましく、より好ましくは1.0μg〜150μg、さらに好ましくは2.0μg〜100μg用いるのが好ましい。該減塩用代用塩を通常の食塩に全量又は一部代用することで、飲食物中の食塩の量を減らしつつ、塩味は落とさないため、食事の美味しさが損なわれることなく、減塩しても満足感が得られる味付けが可能になる。  Moreover, the salt substitute for salt reduction can be manufactured by mixing salt and this salty taste enhancer. It is preferable to use 0.5 μg to 200 μg of piperine, more preferably 1.0 μg to 150 μg, and even more preferably 2.0 μg to 100 μg per 1 g of salt. By substituting the salt for substituting for salt in whole or part with normal salt, the amount of salt in food and drink is reduced and the salty taste is not lost. Even seasoning with satisfaction can be achieved.

本発明の塩味増強剤及び減塩用代用塩は、減圧濃縮、膜濃縮、ドラムドライ、エアードライ、噴霧乾燥、真空乾燥若しくは凍結乾燥、又はそれらの組み合わせ等により、濃縮品や乾燥品とすることができる。  The salty taste enhancer and salt-reducing substitute salt of the present invention shall be concentrated or dried by vacuum concentration, membrane concentration, drum drying, air drying, spray drying, vacuum drying or freeze drying, or a combination thereof. Can do.

以下、実施例を示して本発明を具体的に説明するが、本発明は以下の例によって限定されるものではない。  EXAMPLES Hereinafter, although an Example is shown and this invention is demonstrated concretely, this invention is not limited by the following examples.

(ピペリン濃度の検討)
[試験例1]
水道水225gにアラビアガム(インスタントガムAA、Nexira社製)60gを加えて70℃で撹拌溶解させ、予め黒胡椒抽出物(黒胡椒オレオレジン、CANA株式会社製、ピペリン濃度:40%(w/w))3gと食用油脂(サンクリスタル(登録商標)、日清オイリオグループ株式会社製)12gとを90℃で加熱溶解した混合物を投入した後、高圧ホモジナイザー(LAB−1000、SMT株式会社製)を用いて70℃で乳化処理(40MPa、2Pass)することでピペリン含有乳化物290g(ピペリン濃度:2,300ppm)を得た。
(Examination of piperine concentration)
[Test Example 1]
60 g of gum arabic (instant gum AA, manufactured by Nexira) is added to 225 g of tap water and dissolved by stirring at 70 ° C., and black pepper extract (black pepper oleoresin, manufactured by CANA Corporation, piperine concentration: 40% (w / w)) A mixture of 3 g and 12 g of edible fat (Sun Crystal (registered trademark), manufactured by Nisshin Oillio Group, Inc.) heated and dissolved at 90 ° C. was added, and then a high-pressure homogenizer (LAB-1000, manufactured by SMT Corporation). Was used for emulsification treatment (40 MPa, 2 Pass) at 70 ° C. to obtain 290 g of piperine-containing emulsion (piperine concentration: 2,300 ppm).

水道水67.5gに食塩22.5gと前記ピペリン含有乳化物0.005(実施例1−1)、0.05g(実施例1−2)、0.1g(実施例1−3)、0.5g(実施例1−4)、1g(実施例1−5)、0.001g(比較例1−1)又は5g(比較例1−2)とを加えて70℃で撹拌溶解させ、スプレードライヤー(ADL311、ヤマト科学株式会社製)を用いて噴霧乾燥することで、ピペリン含有食塩粉末各20g(実施品1−1〜1−5、比較品1−1及び1−2)を得た。得られた各食塩粉末中の食塩1gに対するピペリンの量(μg)及び食塩粉末中のピペリン濃度(ppm)を表1に示した。  To 67.5 g of tap water, 22.5 g of sodium chloride and 0.005 (Example 1-1), 0.05 g (Example 1-2), 0.1 g (Example 1-3), 0 .5 g (Example 1-4), 1 g (Example 1-5), 0.001 g (Comparative Example 1-1) or 5 g (Comparative Example 1-2) was added and dissolved by stirring at 70 ° C. By spray-drying using a drier (ADL311, manufactured by Yamato Scientific Co., Ltd.), 20 g each of piperine-containing salt powder (Example products 1-1 to 1-5, Comparative products 1-1 and 1-2) were obtained. Table 1 shows the amount of piperine (μg) and the piperine concentration (ppm) in the salt powder with respect to 1 g of salt in each of the obtained salt powders.

[比較例2]
水道水225gにアラビアガム(インスタントガムAA)60gを加えて70℃で撹拌溶解させ、次いで、食用油脂(サンクリスタル(登録商標))15gを投入した後、高圧ホモジナイザー(LAB−1000)を用いて70℃で乳化処理(40MPa、2Pass)することで、乳化物290gを得た。
[Comparative Example 2]
60 g of gum arabic (instant gum AA) is added to 225 g of tap water and dissolved by stirring at 70 ° C. Then, 15 g of edible oil (Sun Crystal (registered trademark)) is added, and then a high-pressure homogenizer (LAB-1000) is used. By emulsifying at 40 ° C. (40 MPa, 2 Pass), 290 g of an emulsion was obtained.

水道水67.5gに食塩22.5gと前記乳化物0.5gとを加えて70℃で撹拌溶解させ、スプレードライヤー(ADL311)を用いて噴霧乾燥することで、食塩粉末20g(比較品2)を得た。  202.5 g of salt powder (Comparative product 2) is obtained by adding 22.5 g of salt and 0.5 g of the above emulsion to 67.5 g of tap water, stirring and dissolving at 70 ° C., and spray drying using a spray dryer (ADL311). Got.

[評価試験1]
実施品1−1〜1−5、比較品1−1及び1−2を試料として、習熟した4名のパネラーにより、塩味強度及び辛味の官能評価を行い、結果を表1に示した。塩味強度の評価は、比較品2と比較して、相対的に感じる塩味強度を三段階で評価し、塩味強度が「同等」であるものを1、「強い」ものを2、「極めて強い」ものを3として、4名の平均値を算出した。辛味は、辛味の有無で評価した。
[Evaluation Test 1]
Using the practical products 1-1 to 1-5 and the comparative products 1-1 and 1-2 as samples, sensory evaluation of salty strength and pungent taste was performed by four trained panelists, and the results are shown in Table 1. The salty strength is evaluated by comparing the relative salty strength perceived in three stages as compared with Comparative Product 2, with 1 being the “same” salty strength, 2 being “strong”, and “very strong”. The average value of 4 persons was calculated with 3 as the value. The pungent taste was evaluated by the presence or absence of the pungent taste.

Figure 2019150005
Figure 2019150005

評価試験1より、実施品1−1〜1−5は何れも塩味強度が強くなると共に、ピペリン由来の辛味が無かったのに対し、比較品1−1は辛味は無いが、塩味強度はピペリン無添加と同等で、比較品1−2は塩味強度は強くなっていたが、ピペリン由来の辛味が有ることが分かった。  From Evaluation Test 1, all of the products 1-1 to 1-5 had a strong salty strength, and there was no pungent taste derived from piperine, whereas Comparative Product 1-1 had no pungent taste, but the salty strength was piperine. It was found that the comparative product 1-2 had the same salty strength as the additive-free product, but had a pungent taste derived from piperine.

よって、食塩1gに対し、ピペリンを0.5μg以上添加することで塩味増強効果がみられるが、ピペリン添加量が多くなり過ぎると辛味が感じられ、塩味増強効果も低下するため、食塩1gに対し、ピペリンが0.5〜200μg程度が好ましいことが分かった。  Therefore, the salty taste enhancing effect can be seen by adding 0.5 μg or more of piperine to 1 g of salt, but if the amount of piperine added is too much, the pungent taste is felt, and the salty taste enhancing effect is also reduced. Piperine was found to be preferably about 0.5 to 200 μg.

(リモネン添加効果の検討)
[試験例2]
水道水225gにアラビアガム(インスタントガムAA)60gを加えて70℃で撹拌溶解させ、予めd−リモネン(リモネンオイル、株式会社井上香料製造所製、d−リモネン濃度:90%(w/w))3gと食用油脂(サンクリスタル(登録商標))12gとを90℃で加熱溶解した混合物を投入した後、高圧ホモジナイザー(LAB−1000)を用いて70℃で乳化処理(40MPa、2Pass)することでリモネン含有乳化物290g(リモネン濃度:9,000ppm)を得た。
(Examination of limonene addition effect)
[Test Example 2]
60 g of gum arabic (instant gum AA) is added to 225 g of tap water and dissolved by stirring at 70 ° C., and d-limonene (limonene oil, manufactured by Inoue Fragrance Co., Ltd., d-limonene concentration: 90% (w / w) ) After charging a mixture of 3 g and 12 g of edible oil (Sun Crystal (registered trademark)) heated and dissolved at 90 ° C., emulsify (40 MPa, 2 Pass) at 70 ° C. using a high-pressure homogenizer (LAB-1000). Thus, 290 g of limonene-containing emulsion (limonene concentration: 9,000 ppm) was obtained.

水道水67.5gに食塩22.5g、試験例1のピペリン含有乳化物0.5g、及び前記リモネン含有乳化物0.0005(実施例2−1)、0.001(実施例2−2)、0.005g(実施例2−3)、0.01g(実施例2−4)、0.05g(実施例2−5)、0.1g(実施例2−6)又は0.2g(比較例3)を加えて70℃で撹拌溶解させ、スプレードライヤー(ADL311)を用いて噴霧乾燥することで、ピペリン・リモネン含有食塩粉末各20g(実施品2−1〜2−6及び比較品3)を得た。得られた各食塩粉末中の食塩1gに対するピペリンの量(μg)及び各食塩粉末中のリモネン濃度(ppm)、並びにピペリンとリモネンとの比を表2に示した。  62.5 g of tap water 22.5 g of salt, 0.5 g of the piperine-containing emulsion of Test Example 1, and the limonene-containing emulsion 0.0005 (Example 2-1), 0.001 (Example 2-2) , 0.005 g (Example 2-3), 0.01 g (Example 2-4), 0.05 g (Example 2-5), 0.1 g (Example 2-6) or 0.2 g (Comparison) Example 3) was added, dissolved by stirring at 70 ° C., and spray-dried using a spray dryer (ADL311), so that each 20 g of piperine / limonene-containing salt powder (Examples 2-1 to 2-6 and Comparative product 3) Got. Table 2 shows the amount of piperine (μg) per 1 g of salt in each salt powder obtained, the limonene concentration (ppm) in each salt powder, and the ratio of piperine to limonene.

[評価試験2]
実施品2−1〜2−6及び比較品3を試料として、習熟した4名のパネラーにより、塩味強度、辛味及び香りの官能評価を行い、結果を表2に示した。塩味強度の評価は、比較品2と比較して、相対的に感じる塩味強度を三段階で評価し、塩味強度が「同等」であるものを1、「強い」ものを2、「極めて強い」ものを3として、4名の平均値を算出した。辛味は、辛味の有無で評価し、香りは、柑橘系の香りの有無で評価した。
[Evaluation Test 2]
Using Examples 2-1 to 2-6 and Comparative Product 3 as samples, sensory evaluation of salty strength, pungent taste and fragrance was performed by four trained panelists, and the results are shown in Table 2. The salty strength is evaluated by comparing the relative salty strength perceived in three stages as compared with Comparative Product 2, with 1 being the “same” salty strength, 2 being “strong”, and “very strong”. The average value of 4 persons was calculated with 3 as the value. The pungent taste was evaluated by the presence or absence of a pungent taste, and the fragrance was evaluated by the presence or absence of a citrus scent.

Figure 2019150005
Figure 2019150005

評価試験2より、実施品2−1〜2−6は何れも塩味強度が強くなると共に、ピペリン由来の辛味が無く、リモネン由来の甘橘系の香りも無かったのに対し、比較品3は塩味強度は強くなっておりピペリン由来の辛味も無かったが、リモネン由来の柑橘系の香りが有ることが分かった。  From Evaluation Test 2, all of the products 2-1 to 2-6 have a strong salty strength, no pungent taste derived from piperine, and no limonene-derived sweet scent, whereas comparative product 3 is Although the salty strength was strong and there was no pungent taste derived from piperine, it was found that there was a citrus scent derived from limonene.

よって、ピペリンに加え、リモネンを添加することでさらなる塩味増強効果がみられることが分かった。また、リモネン添加量が多くなり過ぎると柑橘系の香りが感じられ、塩味増強効果も低下するため、ピペリン100に対し、リモネンが0.3〜100程度が好ましいことが分かった。  Therefore, it was found that the addition of limonene in addition to piperine shows a further salty taste enhancing effect. Further, when the amount of limonene added is too large, a citrus scent is felt, and the salty taste enhancing effect is also reduced. Therefore, it was found that limonene is preferably about 0.3 to 100 with respect to piperine 100.

(ピペリン高含有品の検討)
[試験例3]
水道水237.552g(実施例3−1)又は233.88g(実施例3−2)に、アラビアガム(インスタントガムAA)60gを各々加えて70℃で撹拌溶解させ、予めd−リモネン(リモネンオイル、d−リモネン濃度:90%(w/w))0.024g(実施例3−1)又は0.06g(実施例3−2)と、黒胡椒抽出物(黒胡椒エキス、バイオアクティブズジャパン株式会社製、ピペリン濃度:96%(w/w))0.024g(実施例3−1)又は0.06g(実施例3−2)と、食用油脂(サンクリスタル(登録商標))2.4g(実施例3−1)又は6g(実施例3−2)とを90℃で加熱溶解した混合物を各々投入した後、高圧ホモジナイザー(LAB−1000)を用いて70℃で乳化処理(40MPa、2Pass)することでピペリン・リモネン含有乳化物各290g(実施例3−1、ピペリン濃度:77ppm、リモネン濃度:72ppm)、(実施例3−2、ピペリン濃度:192ppm、リモネン濃度:180ppm)を得た。
(Examination of high piperine content)
[Test Example 3]
60 g of gum arabic (instant gum AA) was added to 237.552 g of tap water (Example 3-1) or 233.88 g (Example 3-2), respectively, and dissolved by stirring at 70 ° C. In advance, d-limonene (limonene) Oil, d-limonene concentration: 90% (w / w)) 0.024 g (Example 3-1) or 0.06 g (Example 3-2), black pepper extract (black pepper extract, bioactives) Made in Japan, piperine concentration: 96% (w / w)) 0.024 g (Example 3-1) or 0.06 g (Example 3-2), and edible oil (Sun Crystal (registered trademark)) 2 Each mixture of 4 g (Example 3-1) or 6 g (Example 3-2) dissolved by heating at 90 ° C. was added, followed by emulsification (70 MPa) at 70 ° C. using a high-pressure homogenizer (LAB-1000). 2Pass) Rukoto piperine-limonene-containing emulsion the 290 g (Example 3-1, piperine concentration: 77 ppm, limonene concentration: 72 ppm), (Example 3-2, piperine concentration: 192 ppm, limonene concentration: 180 ppm) was obtained.

水道水75gに食塩25gと前記ピペリン・リモネン含有乳化物6.6gとを加えて70℃で撹拌溶解させ、スプレードライヤー(ADL311)を用いて噴霧乾燥することで、ピペリン・リモネン含有食塩粉末各20g(実施品3−1及び3−2)を得た。得られた各食塩粉末中の食塩1gに対するピペリンの量(μg)及び各食塩粉末中のリモネン濃度(ppm)、並びにピペリンとリモネンとの比を表3に示した。  25 g of sodium chloride and 6.6 g of the piperine / limonene-containing emulsion are added to 75 g of tap water, dissolved by stirring at 70 ° C., and spray-dried using a spray dryer (ADL311), each 20 g of piperine / limonene-containing salt powder. (Example products 3-1 and 3-2) were obtained. Table 3 shows the amount of piperine (μg) per 1 g of salt in each salt powder obtained, the limonene concentration (ppm) in each salt powder, and the ratio of piperine to limonene.

[評価試験3]
実施品3−1及び3−2を試料として、習熟した4名のパネラーにより、塩味強度、辛味及び香りの官能評価を行い、結果を表3に示した。塩味強度の評価は、比較品2と比較して、相対的に感じる塩味強度を三段階で評価し、塩味強度が「同等」であるものを1、「強い」ものを2、「極めて強い」ものを3として、4名の平均値を算出した。辛味は、辛味の有無で評価し、香りは、柑橘系の香りの有無で評価した。
[Evaluation Test 3]
Using the practical products 3-1 and 3-2 as samples, sensory evaluation of salty strength, pungent taste, and aroma was performed by four skilled panelists. The results are shown in Table 3. The salty strength is evaluated by comparing the relative salty strength perceived in three stages as compared with Comparative Product 2, with 1 being the “same” salty strength, 2 being “strong”, and “very strong”. The average value of 4 persons was calculated with 3 as the value. The pungent taste was evaluated by the presence or absence of a pungent taste, and the fragrance was evaluated by the presence or absence of a citrus scent.

Figure 2019150005
Figure 2019150005

評価試験3より、実施品3−1及び3−2は何れもピペリン、リモネン無添加の比較品2に比べて塩味強度が増強されていた。実施品3−1及び3−2は何れもピペリン濃度:96%(w/w)のピペリン高含有品を用いており、ピペリン高含有品でも塩味増強効果がみられたことから、ピペリンが塩味増強効果に係る有効成分と考えられた。  From the evaluation test 3, the salty strength of each of the products 3-1 and 3-2 was enhanced as compared with the comparative product 2 to which no piperine and limonene were added. Pipeline-rich products with a piperine concentration of 96% (w / w) were both used in the products 3-1 and 3-2. Since the salty taste-enhancing effect was also observed in the piperine-rich products, piperine was salty. It was considered an active ingredient related to the enhancement effect.

(水溶液における塩味増強効果の確認)
[試験例4]
1.0%(w/w)食塩水200gに、試験例1記載のピペリン含有乳化物(ピペリン濃度:2,300ppm)43mgのみ(実施例4−1)、又は試験例1記載のピペリン含有乳化物(ピペリン濃度:2,300ppm)43mg及び試験例2記載のリモネン含有乳化物(リモネン濃度:9,000ppm)4mg(実施例4−2)を加えて混合することで、ピペリン含有乳化物添加1.0%(w/w)食塩水(実施品4−1、ピペリン濃度:0.5ppm)200g又はピペリン・リモネン含有乳化物添加1.0%(w/w)食塩水(実施品4−2、ピペリン濃度:0.5ppm、リモネン濃度:0.2ppm)200gを得た。得られた各1%食塩水中のピペリン濃度(ppm)及びリモネン濃度(ppm)、食塩1gに対するピペリンの量(μg)、並びにピペリンとリモネンとの比を表4に示した。
(Confirmation of salty taste enhancement effect in aqueous solution)
[Test Example 4]
Piperine-containing emulsion described in Test Example 1 (piperin concentration: 2,300 ppm) only in 43 mg (Example 4-1) or piperine-containing emulsion described in Test Example 1 in 200 g of 1.0% (w / w) saline. The product (piperin concentration: 2,300 ppm) 43 mg and the limonene-containing emulsion described in Test Example 2 (limonene concentration: 9,000 ppm) 4 mg (Example 4-2) were added and mixed to add piperine-containing emulsion 1 0.0% (w / w) saline (practical product 4-1, piperine concentration: 0.5 ppm) 200 g or piperine / limonene-containing emulsion added 1.0% (w / w) saline (practical product 4-2 200 g of piperine concentration: 0.5 ppm, limonene concentration: 0.2 ppm). Table 4 shows the piperine concentration (ppm) and limonene concentration (ppm) in each obtained 1% saline solution, the amount of piperine (μg) with respect to 1 g of sodium chloride, and the ratio of piperine to limonene.

[比較例4]
1.0%(w/w)食塩水200gに、比較例2記載の乳化物47mg(比較例4−1)、黒胡椒抽出物(黒胡椒エキス、バイオアクティブズジャパン株式会社製、ピペリン濃度:96%)0.1mg(比較例4−2)又は試験例2記載のリモネン含有乳化物(リモネン濃度:9,000ppm)4mg(比較例4−3)を加えて混合することで、乳化物添加1.0%(w/w)食塩水(比較品4−1)200g、ピペリン添加1.0%(w/w)食塩水(比較品4−2、ピペリン濃度:0.5ppm)200g又はリモネン含有乳化物添加1.0%(w/w)食塩水(比較品4−3、リモネン濃度:0.2ppm)200gを得た。得られた各1%食塩水中のピペリン濃度(ppm)及びリモネン濃度(ppm)、並びに食塩1gに対するピペリンの量(μg)を表3に示した。
[Comparative Example 4]
In 200 g of 1.0% (w / w) saline, 47 mg of the emulsion described in Comparative Example 2 (Comparative Example 4-1), black pepper extract (black pepper extract, manufactured by Bioactives Japan, Inc., piperine concentration: 96%) 0.1 mg (Comparative Example 4-2) or Limonene-containing emulsion described in Test Example 2 (Limonene concentration: 9,000 ppm) 4 mg (Comparative Example 4-3) was added and mixed to add the emulsion. 200 g of 1.0% (w / w) saline (Comparative product 4-1), 200 g of piperine added 1.0% (w / w) saline (Comparative product 4-2, piperine concentration: 0.5 ppm) or limonene 200 g of containing emulsion addition 1.0% (w / w) saline (Comparative product 4-3, Limonene concentration: 0.2 ppm) was obtained. Table 3 shows the piperine concentration (ppm) and limonene concentration (ppm) in each obtained 1% saline solution, and the amount of piperine (μg) relative to 1 g of sodium chloride.

[評価試験3]
実施品4−1、4−2、比較品4−1、4−2及び4−3の各1.0%(w/w)食塩水を試料として、習熟した4名のパネラーにより、塩味強度の官能評価を行い、結果を表4に示した。塩味強度の評価は、別途調製した標準食塩水1.00%(w/w)、1.05%(w/w)、1.10%(w/w)、1.15%(w/w)、1.20%(w/w)又は1.25%(w/w)と各試料とを比較することで評価した。評価する試料と同じまたは近いと感じる食塩濃度の標準食塩水を決定し、4名の平均値を算出した。
[Evaluation Test 3]
Using each of the 1.0% (w / w) saline solutions of the practical products 4-1 and 4-2 and the comparative products 4-1, 4-2 and 4-3 as samples, The sensory evaluation was performed and the results are shown in Table 4. Evaluation of salty strength is carried out by separately preparing standard saline 1.00% (w / w), 1.05% (w / w), 1.10% (w / w), 1.15% (w / w). ), 1.20% (w / w) or 1.25% (w / w) and each sample was evaluated. A standard saline solution having a salt concentration that is the same as or close to that of the sample to be evaluated was determined, and an average value of four people was calculated.

Figure 2019150005
Figure 2019150005

評価試験4より、実施品4−1及び4−2は何れも食塩濃度が1.05%(w/w)及び1.20%(w/w)相当と、本来の食塩濃度より105%又は120%の塩味増強効果がみられたのに対し、ピペリンを含まない単なる乳化物を添加した比較品4−1、非乳化物のピペリン含有抽出物を添加した比較品4−2及びピペリンを含まないリモネン含有乳化物を添加した比較品4−3は何れも塩味増強効果はみられなかった。  From Evaluation Test 4, the products 4-1 and 4-2 both have a salt concentration equivalent to 1.05% (w / w) and 1.20% (w / w), 105% from the original salt concentration, or 120% salty taste enhancement effect was observed, but comparative product 4-1 to which a simple emulsion not containing piperine was added, comparative product 4-2 to which a non-emulsified piperine-containing extract was added, and piperine were included None of the comparative products 4-3 to which no limonene-containing emulsion was added showed the salty taste enhancing effect.

よって、水溶液の塩味増強効果においては、ピペリン含有乳化物又はさらにリモネンを含むピペリン・リモネン含有乳化物を添加することが重要で、単なる乳化物や非乳化物であるピペリン含有抽出物の添加、またリモネンのみの乳化物では塩味増強効果は見られないことが分かった。さらに、ピペリンにリモネンを加えることで、ピペリンのみに比べ、114%の塩味増強効果がみられることが分かった。  Therefore, in order to enhance the salty taste of the aqueous solution, it is important to add a piperine-containing emulsion or a piperine-limonene-containing emulsion that further contains limonene, the addition of a piperine-containing extract that is a simple emulsion or a non-emulsion, or It was found that the salty taste enhancing effect was not observed with the limonene-only emulsion. Furthermore, it was found that by adding limonene to piperine, a salty taste enhancing effect of 114% was observed compared to piperine alone.

Claims (7)

ピペリンを含む乳化物を有効成分とする、塩味増強剤。  A salty taste enhancer comprising an emulsion containing piperine as an active ingredient. さらにリモネンを含有する、請求項1記載の塩味増強剤。  The salty taste enhancer according to claim 1, further comprising limonene. 水溶性高分子を含有する、請求項1又は2記載の塩味増強剤。  The salty taste enhancer according to claim 1 or 2, comprising a water-soluble polymer. 塩化ナトリウム1gに対し、ピペリンを0.5μg〜200μg用いることを特徴とする、請求項1〜3の何れか1項に記載の塩味増強剤。  The salty taste enhancer according to any one of claims 1 to 3, wherein piperine is used in an amount of 0.5 µg to 200 µg per 1 g of sodium chloride. ピペリン100重量部に対して、リモネン0.3〜100重量部を含む、請求項1〜4の何れか1項に記載の塩味増強剤。  The salty taste enhancer according to any one of claims 1 to 4, comprising 0.3 to 100 parts by weight of limonene with respect to 100 parts by weight of piperine. 請求項1〜5の何れか1項に記載の塩味増強剤を含む、飲食物。  Food / drink containing the salty taste enhancer of any one of Claims 1-5. 塩化ナトリウムと請求項1〜5の何れか1項に記載の塩味増強剤とを含む、減塩用代用塩。  A salt substitute for reducing salt, comprising sodium chloride and the salty taste enhancer according to any one of claims 1 to 5.
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