JP2019141090A5 - - Google Patents
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- JP2019141090A5 JP2019141090A5 JP2019087392A JP2019087392A JP2019141090A5 JP 2019141090 A5 JP2019141090 A5 JP 2019141090A5 JP 2019087392 A JP2019087392 A JP 2019087392A JP 2019087392 A JP2019087392 A JP 2019087392A JP 2019141090 A5 JP2019141090 A5 JP 2019141090A5
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- embryo
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- blastomeres
- selection system
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- 210000001161 Embryo, Mammalian Anatomy 0.000 claims description 37
- 210000001109 Blastomeres Anatomy 0.000 claims description 15
- 230000004720 fertilization Effects 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 238000003776 cleavage reaction Methods 0.000 claims description 7
- 210000002257 embryonic structures Anatomy 0.000 claims description 6
- 230000001537 neural Effects 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 description 2
- 238000010187 selection method Methods 0.000 description 1
Description
本発明は、上述の課題の少なくとも一部を解決するためになされたものであり、以下の形態として実現することが可能である。第１の態様は、胚選抜システムとしての態様である。この胚培養システムは、培養容器に設けられた複数のウェルに収容され培養されている胚を、設定された時間間隔で撮像した前記ウェル毎の画像を、時系列的に格納する画像格納部と、前記画像格納部に格納された前記画像を読み出して、前記画像における前記胚の割球数の経時的な変化を解析することによって前記胚の状態を判定する判定部と、を備え、前記判定部は、受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態に直接移行したことを確認した場合と、受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態を経ることなく前記胚の割球数が３個以上の状態に直接移行した場合とで、前記胚について異なる判定を行なう。
また、第２の態様は、胚を選抜する方法としての態様である。この胚選抜方法は、培養容器に設けられた複数のウェルに収容され培養されている胚を、設定された時間間隔で撮像した前記ウェル毎の画像を、時系列的に格納する画像格納工程と、前記画像格納工程で格納された前記画像を読み出して、前記画像における前記胚の割球数の経時的な変化を解析することによって胚を選抜する選抜工程と、を備え、前記選抜工程において、受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態に直接移行したことを確認したか、あるいは受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態を経ることなく前記胚の割球数が３個以上の状態に直接移行したか、によって前記胚を選抜する。
The present invention has been made to solve at least a part of the above-mentioned problems, and can be realized as the following forms. The first aspect is an aspect as an embryo selection system. This embryo culture system includes an image storage unit that stores images of the embryos housed and cultured in a plurality of wells provided in a culture container at set time intervals for each well in chronological order. The determination unit is provided with a determination unit for determining the state of the embryo by reading the image stored in the image storage unit and analyzing the change in the number of blastomeres of the embryo in the image with time. In the first egg splitting started after the fertilization process, the part confirmed that the number of blastomeres of the embryo directly shifted to the two state, and the first egg split started after the fertilization process. In the split, different determinations are made for the embryo depending on whether the number of blastomeres of the embryo directly shifts to the state of 3 or more without going through the state of 2.
The second aspect is an aspect as a method for selecting embryos. This embryo selection method includes an image storage step of storing images of the embryos housed and cultured in a plurality of wells provided in a culture container at set time intervals for each well in chronological order. A selection step of reading out the image stored in the image storage step and selecting an embryo by analyzing a change in the number of blastomeres of the embryo in the image over time is provided, and the selection step includes. In the first cleavage started after the fertilization process, it was confirmed that the number of blastomeres of the embryo directly shifted to the two state, or in the first cleavage started after the fertilization treatment, The embryo is selected depending on whether the number of blastomeres of the embryo directly shifts to the state of 3 or more without going through the state of 2 blastomeres.
Claims (6)
培養容器に設けられた複数のウェルに収容され培養されている胚を、設定された時間間隔で撮像した前記ウェル毎の画像を、時系列的に格納する画像格納部と、
前記画像格納部に格納された前記画像を読み出して、前記画像における前記胚の割球数の経時的な変化を解析することによって前記胚の状態を判定する判定部と、
を備え、
前記判定部は、受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態に直接移行したことを確認した場合と、受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態を経ることなく前記胚の割球数が３個以上の状態に直接移行した場合とで、前記胚について異なる判定を行なう、胚選抜システム。 Embryo selection system
An image storage unit that stores images of embryos housed and cultured in a plurality of wells provided in a culture container in a time-series manner for each well, which are images taken at set time intervals.
A determination unit for determining the state of the embryo by reading out the image stored in the image storage unit and analyzing a change in the number of blastomeres of the embryo in the image over time.
Equipped with a,
In the first cleavage started after the fertilization process, the determination unit confirms that the number of blastomeres of the embryo has directly shifted to the two state, and the first cleavage process is started after the fertilization process. In the cleavage of the embryo, different judgments are made for the embryo depending on whether the number of blastomeres of the embryo directly shifts to the state of 3 or more without going through the state of 2 blastomeres. system.
前記判定部は、教師あり学習によって前記画像に写った前記胚の特徴について学習し、前記学習に基づいて前記胚の状態を判定する、胚選抜システム。 The embryo selection system according to claim 1.
The determination unit is an embryo selection system that learns the characteristics of the embryo captured in the image by supervised learning and determines the state of the embryo based on the learning.
前記判定部は、多層のニューラルネットワークを用いたディープラーニングによって前記画像に写った前記胚の特徴について学習し、前記学習に基づいて前記胚の状態を判定する、胚選抜システム。 The embryo selection system according to claim 1.
The determination unit is an embryo selection system that learns the characteristics of the embryo captured in the image by deep learning using a multi-layer neural network and determines the state of the embryo based on the learning.
前記画像のうち判定に用いられた判定画像を報知する報知部を備える、胚選抜システム。 The embryo selection system according to any one of claims 1 to 3, and further.
An embryo selection system including a notification unit for notifying a determination image used for determination among the images.
前記報知部は、前記判定画像を報知するとともに前記判定画像において前記判定部が判定の根拠とした情報を報知する、胚選抜システム。 The embryo selection system according to claim 4.
The notification unit is an embryo selection system that notifies the determination image and also notifies the information on which the determination unit is based in the determination image.
培養容器に設けられた複数のウェルに収容され培養されている胚を、設定された時間間隔で撮像した前記ウェル毎の画像を、時系列的に格納する画像格納工程と、
前記画像格納工程で格納された前記画像を読み出して、前記画像における前記胚の割球数の経時的な変化を解析することによって胚を選抜する選抜工程と、
を備え、
前記選抜工程において、受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態に直接移行したことを確認したか、あるいは受精処理されてから開始される最初の卵割において、前記胚の割球数が２個の状態を経ることなく前記胚の割球数が３個以上の状態に直接移行したか、によって前記胚を選抜する、方法。 It ’s a method of selecting embryos .
An image storage step of chronologically storing images for each well , in which embryos housed and cultured in a plurality of wells provided in a culture container are imaged at set time intervals,
Said reading the image stored in the image storing step, a selection step of selecting a embryo by the analyzing changes over time in the number of blastomeres the embryo in the image,
Equipped with a,
In the selection step, in the first cleavage started after the fertilization process, it was confirmed that the number of blastomeres of the embryo directly shifted to the two state, or the first started after the fertilization process. A method of selecting an embryo according to whether or not the number of blastomeres of the embryo directly shifts to a state of 3 or more without going through the state of 2 blastomeres in the cleavage .
Priority Applications (1)
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|---|---|---|---|
| JP2019087392A JP7000379B2 (en) | 2019-05-07 | 2019-05-07 | Embryo selection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019087392A JP7000379B2 (en) | 2019-05-07 | 2019-05-07 | Embryo selection system |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017236640A Division JP6732722B2 (en) | 2017-12-11 | 2017-12-11 | Embryo selection system |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019141090A JP2019141090A (en) | 2019-08-29 |
| JP2019141090A5 true JP2019141090A5 (en) | 2021-01-28 |
| JP7000379B2 JP7000379B2 (en) | 2022-01-19 |
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Country Status (1)
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Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010004789A (en) * | 2008-06-26 | 2010-01-14 | Nikon Corp | Embryo observation apparatus |
| JP5807288B2 (en) * | 2010-06-30 | 2015-11-10 | 大日本印刷株式会社 | Method for producing embryo by in vitro culture, and method, apparatus, and system for selecting embryo |
| US10628944B2 (en) * | 2014-07-01 | 2020-04-21 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for three dimensional reconstruction and determining the quality of an embryo |
| US10180387B2 (en) * | 2014-07-29 | 2019-01-15 | National University Corporation Hamamatsu University School Of Medicine | Identification device and identification method |
| GB2531699A (en) * | 2014-10-03 | 2016-05-04 | Unisense Fertilitech As | Embryo Assessment |
| JP6528608B2 (en) * | 2015-08-28 | 2019-06-12 | カシオ計算機株式会社 | Diagnostic device, learning processing method in diagnostic device, and program |
| WO2017150194A1 (en) * | 2016-03-04 | 2017-09-08 | コニカミノルタ株式会社 | Image processing device, image processing method, and program |
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