JP2019126344A - Food composition for promoting gene expression related to food factor sensing,food factor sensing related gene expression promoter,food composition for enhancing food factor function,food factor function enhancer and food additives for enhancing food factor function - Google Patents

Abstract

To provide a food composition for promoting gene expression related to food factor sensing and a food factor sensing related gene expression promoter.SOLUTION: The food composition for promoting gene expression related to food factor sensing and the food factor sensing related gene expression promoter contain an Euglena-derived substance as an active ingredient and are used to promote expression of food factor sensing-related genes.SELECTED DRAWING: Figure 5

Description

  The present invention relates to a food composition for promoting food factor sensing-related gene expression, a food factor sensing-related gene expression promoter, a food composition for enhancing food factor function, a food factor function enhancing agent, and a food additive for enhancing food factor function.

  In recent years, awareness of health has been increasing year by year, and research on health has been actively conducted in the food field. Food has a primary function of life support that supplements nutrition, a secondary function that satisfies taste and taste, and a tertiary function that regulates the body's physiological system to maintain and restore health . In recent years, this tertiary function has attracted attention, and many foods for specified health use, functional nutritional foods, and functional labeling foods have been developed.

  As foods for specified health use, many foods that are said to be effective in suppressing the increase in blood pressure and blood sugar level and the absorption of sugar and body fat are being sold. However, the details of the mechanism of action of functional ingredients contained in foods for specified health use have not been elucidated.

  In research on the mechanism of action of food functional ingredients, recent reports report that food functional ingredients bind to intracellular and extracellular receptors, and that the function is exhibited after the binding (Non-patent Document 1). This is because a functional component of food binds to a specific target molecule to transmit a signal into the cell and exert its effect. Depending on the presence or expression level of the target molecule on the receiving side, the same food component Even shows that the effect is different.

On the other hand, Euglena (genus name: Euglena, Japanese name: Euglena) attracts attention as a biological resource that is expected to be used as food, feed, fuel, and the like.
Euglena is a supplement for the well-balanced intake of many types of nutrients, with 59 types of nutrients corresponding to most of the nutrients necessary for human beings to live including vitamins, minerals, amino acids, unsaturated fatty acids, etc. It has been suggested that it could be used as a food source in poor areas where it can not consume necessary nutrients.

Euglena is located in the primary producer of the food chain and is cultivated in large quantities because it is preyed by predators and the culture conditions such as light, temperature conditions, and stirring speed are difficult compared to other microorganisms. However, in recent years, due to the diligent research of the present inventors, a large-scale culture technique has been established, which has opened the way for mass supply of Euglena-derived substances such as Euglena and paramylon extracted from Euglena.
Euglena is a unique organism that possesses the animal nature of flagellar movement and at the same time has chloroplasts as plants and performs photosynthesis, and many functionalities possessed by Euglena itself and substances derived from Euglena have been reported. (Non-patent document 2). The functions of Euglena itself and substances derived from Euglena and the mechanism of action in the body are still unknown in detail in many parts, and the creation of new functions and the clarification of the mechanism of action in the body are required.

H. Tachibana, et al., Nat. Struct. Mol. Biol., Vol.11, pp.380-381 (2004). Ryo Arata, `` Characteristics of microalgae Euglena and its application to food and environmental fields '', Photosynthesis Research, vol.22, no.1, pp.33-38 (2012).

The present invention has been made in view of the above-mentioned problems, and the object of the present invention is to be able to continuously ingest daily and effectively promote the expression of food factor sensing-related genes. An object of the present invention is to provide a food composition for promoting food factor sensing-related gene expression and a food factor sensing-related gene expression promoter.
Another object of the present invention is to provide a food composition for enhancing food factor function, a food factor function enhancing agent, and a food additive for enhancing food factor function capable of enhancing the food factor function of food factor. It is in.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that Euglena-derived substances have an action of promoting the expression of food factor sensing-related genes, and have completed the present invention.

  Therefore, according to the present invention, the object of the present invention is to provide a food factor sensing-related gene expression promoting food comprising a Euglena-derived substance as an active ingredient and used for promoting the expression of a food factor sensing-related gene. Solved by the composition.

  At this time, the Euglena-derived substance may be at least one selected from the group consisting of Euglena or Paramylon.

  At this time, the Euglena-derived substance is Euglena, and Adora2a (SEQ ID NO: 1), Adora3 (SEQ ID NO: 2), Adra1a (SEQ ID NO: 3), Bcl2 (SEQ ID NO: 4), Ccr2 (SEQ ID NO: 5), Ccr5 in the liver (SEQ ID NO: 6), Ccr6 (SEQ ID NO: 7), Cd36 (SEQ ID NO: 8), Ffar1 (SEQ ID NO: 9), Gabbr1 (SEQ ID NO: 10), Glra4 (SEQ ID NO: 11), Hic2 (SEQ ID NO: 12), IL1a ( Sequence number 13), IL1b (sequence number 14), IL6 (sequence number 15), IL16 (sequence number 16), Ppara (sequence number 17), Tlr1 (sequence number 18), Tlr3 (sequence number 19), Tlr5 (sequence) No. 20) and Tlr6 (SEQ ID NO: 21) may be used to increase the expression level of one or more genes selected from the group consisting of.

  At this time, the Euglena-derived substance is paramylon, and Adora3 (SEQ ID NO: 2), Adra1a (SEQ ID NO: 3), Ager (SEQ ID NO: 22), Bcl2 (SEQ ID NO: 4) Ccr5 (SEQ ID NO: 6), Cd4 (in the liver) SEQ ID NO: 23), Esrrb (SEQ ID NO: 24), IL1b (SEQ ID NO: 14), IL1r1 (SEQ ID NO: 25), Ltb4r1 (SEQ ID NO: 26), Tlr6 (SEQ ID NO: 21), Olr1 (SEQ ID NO: 27) and Stx3 (sequence) It may be used for increasing the expression level of one or more genes selected from the group consisting of No. 28).

  At this time, the Euglena-derived substance is Euglena, and Bcl2l1 (SEQ ID NO: 29), Ccr6 (SEQ ID NO: 7), Esr1 (SEQ ID NO: 30), Esrrb (SEQ ID NO: 24), Ffar3 (SEQ ID NO: 31), Glrb in muscle (SEQ ID NO: 32), Ppara (SEQ ID NO: 17), Rxra (SEQ ID NO: 33), IL6ra (SEQ ID NO: 34), Tlr6 (SEQ ID NO: 21) and one or more selected from the group consisting of Vdr (SEQ ID NO: 35) It may be used to increase the expression level of the gene.

  At this time, the Euglena-derived substance is paramylon, and Bcl2l1 (SEQ ID NO: 29), Gabbr1 (SEQ ID NO: 10), Gpr84 (SEQ ID NO: 36), IL6ra (SEQ ID NO: 33), IL16 (SEQ ID NO: 16), Sirt1 in muscle It may be used to increase the expression level of one or more genes selected from the group consisting of (SEQ ID NO: 37) and Tlr4 (SEQ ID NO: 38).

In addition, according to the present invention, there is provided a food factor sensing-related gene expression promoter characterized by containing a Euglena-derived substance as an active ingredient and used for promoting the expression of a food factor sensing-related gene. Solved.
At this time, the Euglena-derived substance is preferably at least one selected from the group consisting of Euglena and paramylon.

In addition, according to the present invention, the above-mentioned problem is solved by a food composition for enhancing food factor function, which contains a Euglena-derived substance as an active ingredient and is used for enhancing food factor function.
Further, according to the present invention, the above-mentioned problems are solved by a food factor function enhancer characterized in that it contains an Euglena-derived substance as an active ingredient and is used to enhance a food factor function.
Moreover, according to this invention, the said subject is solved by the food additive for food factor function enhancement characterized by containing the Euglena origin substance as an active ingredient, and being used in order to enhance food factor function.
In addition, according to the present invention, the above-described problem is solved by a food composition for enhancing food factor function, which comprises Euglena as an active ingredient and is used for enhancing the food factor function of soybean isoflavone. .
In addition, according to the present invention, the above-mentioned problem is solved by a food additive for enhancing food factor function, which contains Euglena as an active ingredient and is used for enhancing the food factor function of soybean isoflavone. .

According to the present invention, a novel food composition for promoting food factor sensing-related gene expression, a food factor sensing-related gene expression promoting agent, a food composition for enhancing food factor function, a food factor function enhancing agent, and a food for enhancing food factor function Additives can be provided.
Food factor sensing-related gene expression promoting food composition, food factor sensing-related gene expression promoting agent, food factor function enhancing food composition, food factor function enhancing agent and food factor function enhancing food additive of the present invention Since there are no reports of side effects and euglena-derived substances with a safety level consistent with the Food Sanitation Law are used as active ingredients, long-term continuous administration and continuous ingestion are possible.

It is a graph which shows the change of the gene expression level in the liver by ingestion of a Euglena origin substance. It is a graph which shows the change of the gene expression level in the liver by ingestion of a Euglena origin substance. It is a graph which shows the change of the gene expression level in the liver by ingestion of a Euglena origin substance. It is a graph which shows the change of the gene expression level in the liver by ingestion of a Euglena origin substance. It is a figure which shows the food factor sensing related gene which the expression increased in the liver. It is a graph which shows the change of the gene expression level in quadriceps femoris by ingestion of a Euglena origin substance. It is a graph which shows the change of the gene expression level in quadriceps femoris by ingestion of a Euglena origin substance. It is a graph which shows the change of the gene expression level in quadriceps femoris by ingestion of a Euglena origin substance. It is a figure which shows the food factor sensing related gene which expression was raised by the quadriceps femoris muscle. It is a conceptual diagram for demonstrating an example of the food factor function enhancement effect of Euglena. It is a conceptual diagram for demonstrating an example of the food factor function enhancement effect of paramylon. It is a graph which shows the measurement result of Esr1 and Esrrb gene expression level in quadriceps femoris (Data area means ± SE, n = 5, Student's t-test, * p <0.05 vs. control). It is a graph which shows the measurement result of the Ctsd gene expression level in quadriceps femoris (Data area means ± SE, n = 5, Student's t-test, * p <0.05 vs. control).

Hereinafter, embodiments of the present invention will be described with reference to FIGS.
The present embodiment comprises a food composition for promoting food factor sensing-related gene expression, a food factor sensing-related gene expression promoting agent, a food composition for enhancing food factor function, a food factor function enhancing agent, which contains a Euglena-derived substance as an active ingredient, and The present invention relates to a food additive for enhancing food factor function.

<Food factor>
The food factor (food factor) is a group of substances that play an important role in the "physiological and physiological regulation function", which is the tertiary function of food, and is synonymous with "functional food factor".
A food factor (functional food factor) is a physiologically active substance that regulates physiological systems in the body and has an effect on maintaining and restoring health.

  The classification of functional food factors is not uniform, but there are classifications by food ingredient, classification by target disease, classification based on the field of action (biological system), classification based on action mechanism, etc. (Science Editorial Committee for Food Functionality, “Science for Food Functionality”, April 2008).

(Classification by food ingredient)
About the example of classification according to food ingredients, examples of functional food factors are shown below for each food ingredient group, but functional food factors are not limited to those shown below.
-Carbohydrate: Indigestible polysaccharides, oligosaccharides, etc.-Lipids: Polyunsaturated fatty acids, plant sterols, glycerophospholipids, sphingolipids, etc.-Proteins: Functional proteins such as milk proteins and soy proteins, oligopeptides, amino acids etc.・ Vitamin: vitamin E (tocopherol, tocotrienol), vitamin K (menaquinone), folic acid etc. ・ Mineral: calcium, magnesium, iron ・ nucleotide: AMP (adenylate), CMP (cytidylic acid), GMP (guanylate), IMP Inosinic acid)
-Carotenoid: Carotene, lycopene-Isoprenoid: Terpene-Polyphenol: Flavonoid, isoflavone, anthocyanidin-Sulfur-containing compound: Isothiocyanate-Microorganism: Bifidobacterium, lactic acid bacteria

(Classification by target disease)
Examples of functional food factors for each disease are shown below for examples of target diseases, but functional food factors are not limited to those shown below.
Hypertension: peptide, geniposide acid, acetic acid, n-3 fatty acid hypercholesterolemia: soy protein, peptide, chitosan, alginic acid, polyphenol, plant sterol, lactic acid bacteria, hyperglyceremia: tea catechins, globin peptides, isoprenoid Hyperglycemia, diabetes: wheat albumin, indigestible dextrin, tea polyphenol, isoprenoid · arteriosclerosis, cardiovascular disease: carotenoids, polyphenols, n-3 fatty acids · obesity: tea catechins, diacylglycerol · cancer: carotenoids, flavonoids, Isoflavones, isothiocyanates, lactic acid bacteria, n-3 fatty acids, glycosphingolipids, osteoporosis: basic milk proteins, isoflavones, lactic acid bacteria, oligosaccharides, menaquinone, dental caries: xylitol, calcium monohydrogen phosphate, constipation: difficult Dextrin, oligosaccharide, diarrhea: indigestible dextrin, oligosaccharide, inflammatory enteritis: amino acid, polyphenol, lactic acid bacteria, liver injury: isoprenoid, amino acid, gastritis: milk protein, digestive tract infection: peptide, antibody protein, lactic acid bacteria -Asthma-Atopy: polyphenols, lactic acid bacteria, oligosaccharides, n-3 fatty acids, glycosphingolipids-hay fever: polyphenols, lactic acid bacteria, oligosaccharides-dementia: lecithin, n-6 fatty acids, n-3 fatty acids, eyes Fatigue: Astaxanthin, Anthocyanin / Insomnia: Glycine

(Classification based on the field where it acts)
The classification based on the field of action (classification by biological system) is a classification that was proposed when the concept of the tertiary function of food was proposed. Immune system regulator, secretory regulator, nervous system regulator The classification is divided into six types of factors, circulatory system regulators, digestive system regulators, and cell system regulators.

(Classification based on action mechanism)
Classification based on the mechanism of action includes induction of changes in the intestinal bacterial flora, regulation of mass transfer rate in the intestinal tract, binding / adsorption of nutrients in the intestinal tract, antibacterial action, inhibition of enzymes, activity of enzymes and proteins It is a classification based on action mechanisms such as oxidization, antioxidant action and modification of hormone action.

<Food factor function>
The food factor function is a function that the food factor exhibits in the living body. Various food factor functions have been found for each food factor, such as lipid metabolism improving action of unsaturated fatty acids, bone protecting action of vitamin D, anti-diabetic action of long chain fatty acids, anti-aging action of flavonoids, γ -Blood pressure lowering action of aminobutyric acid (GABA), adrenergic action of cineferin, etc. are known.

<Food factor sensing related gene (food factor sensing related gene)>
The food factor sensing related gene (food factor sensing related gene) is a gene on the living body responsible for functional expression of the food factor. Food factor sensing related genes are genes involved in food factor sensing systems in the living body, and various genes are known for each food factor. Examples of food factor sensing related genes, along with their food factors and characteristics, are shown in Tables 1-4 below.

<Euglena>
In this embodiment, “Euglena” includes taxonomically classified microorganisms classified as Euglena, variants thereof, variants thereof, and closely related species of Euglenaaceae.
Here, Euglena (Euglena) is a group of organisms belonging to Excata, Euglenozoa, Euglena, Euglena, Euglenidae among eukaryotes.

Specific examples of Euglena species include Euglena chadefaudii, Euglena deses, Euglena gracilis, Euglena granulata, Euglena mutabilis, Euglena probila, Euglena spirogyra, Euglena viridis and the like.
As Euglena, E. gracilis (E. gracilis), in particular, E. gracilis (E. gracilis) Z strain can be used, but in addition, a mutant SM-ZK strain of E. gracilis Z strain. It may be a mutant of E. gracilis var. Bacillaris (a chloroplast-deficient strain) or a variant, a gene mutant such as a chloroplast mutant of these species, or another euglena such as Astaia longa.

Euglena is widely distributed in fresh water such as ponds and swamps, and may be used separately from these, or any Euglena already isolated may be used.
Euglena includes all its mutants. Further, among these mutant strains, those obtained by genetic methods such as recombination, transduction, transformation and the like are also contained.

In the culture of Euglena cells, as a culture solution, for example, a culture solution to which nutrients such as nitrogen source, phosphorus source, and minerals have been added, for example, modified Cramer-Myers medium ((NH ₄ ) ₂ HPO ₄ 1.0 g / L , KH ₂ PO ₄ 1.0 g / L, MgSO ₄ · 7H ₂ O 0.2 g / l, CaCl ₂ · 2H ₂ O 0.02 g / l, Fe ₂ (SO ₂ ) ₃ · 7H ₂ O 3 mg / l, MnCl ₂ · 4H ₂ O 1.8 mg / l, CoSO ₄ · 7H ₂ O 1.5 mg / l, ZnSO ₄ · 7H ₂ O 0.4 mg / l, Na ₂ MoO ₄ · 2H ₂ O 0.2 mg / l, CuSO ₄ .5H ₂ O 0.02 g / l, thiamine hydrochloride (vitamin B1) 0.1 mg / l, cyanocobalamin (vitamin B12), (pH 3.5)) can be used. (NH ₄ ) ₂ HPO ₄ can also be converted to (NH ₄ ) ₂ SO ₄ or NH ₃ aq. In addition, a known Hutner medium or Koren-Hutner medium prepared based on the description of Euglena Physiology and Biochemistry (edited by Shozaburo Kitaoka, Society Publishing Center, Inc.) may be used.

The pH of the culture solution is preferably 2 or more, and the upper limit thereof is preferably 6 or less, more preferably 4.5 or less. By setting the pH to the acidic side, the photosynthetic microorganisms can grow more dominantly than other microorganisms, so that contamination can be suppressed.
Euglena cells may be cultured by an open pond method using sunlight directly, a light collecting method in which sunlight condensed by a light concentrator is sent through an optical fiber, etc., and irradiated in a culture tank and used for photosynthesis.
Euglena cells can be cultured using, for example, a fed-batch method, but flask culture, fermentation using a fermentor, batch culture, semi-batch culture (fed-batch culture), continuous culture (perfusion) It may be carried out by any liquid culture method such as culture method).
The separation of Euglena cells is performed, for example, by centrifugation, filtration or simple sedimentation of the culture fluid.

(Euglena algae)
In the present embodiment, viable Euglena cells separated by centrifugation, filtration, sedimentation, or the like can be used as they are as Euglena alga bodies. Euglena viable cells can be used as they are after harvesting from the culture tank, but are preferably washed with water or physiological saline. Also, it may be used in the form of a dispersion in which Euglena algal cells are dispersed in a liquid such as water. In the present embodiment, it is preferable to use the dried algal cells of Euglena obtained by freeze-drying treatment or spray-drying treatment of Euglena live cells as Euglena algal cells.
Furthermore, a mechanically treated product of algal cells obtained by performing ultrasonic irradiation treatment or mechanical treatment such as homogenization may be used as the euglena algal cells. Moreover, you may use the mechanical-process dried material which performed the dry process to the mechanical process as an Euglena algal body.

(Euglena aqueous solvent extract)
In the present embodiment, “Euglena aqueous solvent extract” means an extract extracted from Euglena using an aqueous solvent, and in particular, water is used as an aqueous solvent at 5 ° C. to 600 ° C. for several seconds to several times. It is preferred to use a water extract or a hot water extract of Euglena extracted for 10 hours.
The water used for extraction does not necessarily need to be distilled water, pure water, or ultrapure water. For example, it may contain tap water or impurities, but it contains components that hinder the extraction of active ingredients. No water is preferred.

In the present embodiment, the “water extract” means an extract with water of 0 to 50 ° C. (excluding 0 ° C.).
Here, "water" means water of 0 to 50 ° C (excluding 0 ° C).
The temperature of water is not particularly limited as long as the active ingredient can be sufficiently extracted without affecting the active ingredient, but preferably 1 to 40 ° C., more preferably 5 to 35 ° C. Especially preferably, it is 10-30 degreeC.

In the present embodiment, the “hot water extract” means an extract with water having a temperature higher than 50 ° C., and can also be referred to as a “hot water extract”.
Here, “hot water” means water having a temperature higher than 50 ° C., and is a concept including “hot water”, and includes water in a boiling state. Moreover, it is not limited to hot water in a liquid state, and includes hot water in a gas state and a supercritical state.
The temperature of the hot water is not particularly limited as long as the active ingredient can be sufficiently extracted without affecting the active ingredient, but is preferably higher than 50 ° C. and 120 ° C. or less, more preferably 50 It is higher than 100 ° C. and lower than 100 ° C.

  The pH of water used for extraction is not particularly limited as long as the active ingredient can be sufficiently extracted without affecting the active ingredient, but is preferably 4 to 10, more preferably 5 to 9. The pH is particularly preferably 6 to 8.

  In this embodiment, water is used alone as the aqueous solvent, but the active ingredient can be sufficiently extracted without affecting the active ingredient, and usually one kind of solvent that can be used for extraction is used. Or you may select and use 2 or more types. For example, although water, alcohol, glycols etc. can be mentioned, it is not limited to this. As alcohols, ethanol, methanol, n-propanol, isopropanol etc. are mentioned. Examples of glycols include butylene glycol and propylene glycol. Other aqueous solvents include acetone and the like. These solvents may be used alone or as an aqueous solution, and may be used as any two or three or more mixed solvents.

The temperature of the aqueous solvent used for extraction is, for example, 0 ° C. or higher, and is not particularly limited as long as it does not affect the active ingredient. Although an aqueous solvent in a boiling or supercritical state can be used, it is preferable to use an aqueous solvent at 5 ° C. to 600 ° C., and more preferable to use an aqueous solvent at 10 ° C. to 200 ° C.
Therefore, the aqueous solvent for extraction includes an aqueous solvent in a boiling state or a supercritical state. The amount of aqueous solvent used for extraction is preferably an amount capable of sufficiently dissolving the water-soluble active ingredient contained in Euglena.

The extraction method is also not particularly limited. For example, extraction can be performed by the following method, but the extraction method is not limited to this, and a normal extraction method can be freely selected and used. For example, a method of centrifuging or filtering Euglena alga body dry powder in an aqueous solvent for a predetermined time and then centrifuging or filtering; Euglena alga body dry powder added to an aqueous solvent and shaken to uniformly disperse, and then centrifuged or filtered. Method, etc.
It is also possible to heat the aqueous solvent after the addition of Euglena to facilitate extraction.

Euglena water extraction can be carried out by the usual method as shown below, but is not limited thereto. For example, the Euglena tissue and water are placed in a container and allowed to stand for a predetermined time with appropriate stirring or shaking, and the resulting extract can be used as a water extract as it is. In addition, for example, a supernatant obtained by centrifuging such an extract can be used as a water extract. Alternatively, such extract or supernatant may be concentrated, dried to remove water and used as a water extract. Water extraction may be performed by adding a small amount of alcohol, for example, 10% by mass or less, preferably ethanol, to water in order to increase extraction efficiency and shorten extraction time.
The extraction time in the case of water extraction is not particularly limited as long as it is a time during which the active ingredient is extracted, and can be appropriately set according to the temperature of extraction within a range of several seconds to several tens of hours.

Extraction with hot water can be carried out by a commonly used method as shown below, but it is not limited thereto. Extraction is carried out by heating Euglana after introducing it into a commonly used extractor together with water. When extracting using boiling water or water in a supercritical state, it is necessary to use an extractor that can withstand the vapor pressure of water. The pressure at the time of extraction can be set to 1 to 5000 atmospheres, and is preferably set to 60 to 400 atmospheres.
In the case of extraction under high temperature and pressure, if the extraction time is too long, the active ingredient may be decomposed or a chemical reaction may occur. Therefore, when extraction is performed under high temperature and high pressure, the extraction time is preferably short, for example, 3 minutes or less, more preferably 1 minute or less, and particularly preferably 30 seconds or less.

Although the extracted Euglena extract can be used as it is as an active ingredient such as a food factor sensing-related gene expression promoter according to the present embodiment, the extract can be further used in an appropriate separation means (for example, distribution extraction, gel It is also possible to fractionate and use a fraction with high activity by a filtration method, silica gel chromatography, reverse phase or normal phase high performance liquid chromatography, and the like.
Alternatively, the Euglena extract or a fraction thereof may be concentrated and dried to remove an aqueous solvent, which may be used as an aqueous solvent extract.

(Paramilon)
“Paramylon” is a high molecular weight polymer (β-1,3-glucan) in which about 700 glucoses are polymerized by β-1,3-linkage, and is a storage polysaccharide contained in the genus Euglena. It is. Paramylon particles are flat spheroid particles, and β-1,3-glucan chains are entangled in a spiral shape.

Paramylon exists as granules in Euglena cells of all species and varieties, and the number, shape, and uniformity of the particles are characterized by species.
Paramylon consists only of glucose, and the average degree of polymerization of paramylon obtained from a wild strain of E. gracilis Z and a chloroplast-deficient strain SM-ZK is about 700 in glucose units.
Paramylon is insoluble in water and hot water, but is soluble in dilute alkali, concentrated acid, dimethyl sulfoxide, formaldehyde, and formic acid.
The average density of paramylon is 1.53 for E. gracilis Z and 1.63 for E. gracilis var. Bacillaris SM-L1.
Paramylon has a gentle helical structure in which three linear β-1,3-glucans are twisted like a right-handed rope according to X-ray analysis using a powder pattern method. Several of these glucan molecules gather to form paramylon granules. Paramylon granules have a very large crystal structure and occupy about 90%, and are the compounds with the highest crystal structure ratio among the polysaccharides (Euglena Physiology and Biochemistry, edited by Shozaburo Kitaoka, Academic Publishing Center).
In addition, the particle size distribution of paramiron (made by Euglena Co., Ltd.) is 1.5-2.5 micrometers in median diameter when it measures with laser diffraction / scattering type particle size distribution measuring apparatus.

Paramylon particles are isolated from the cultured Euglena cells by any appropriate method and purified into fine particles, and are usually provided as powders.
For example, paramylon particles are (1) culture of Euglena cells in any appropriate medium, (2) separation of Euglena cells from the medium, (3) isolation of paramylon from separated Euglena cells, 4) It can be obtained by purification of the isolated paramylon, and optionally (5) cooling and subsequent lyophilization.
The isolation of paramylons is carried out, for example, using non-ionic or anionic surfactants of the type which are mostly biodegradable. The purification of paramylon is performed substantially simultaneously with the isolation.

  The isolation and purification of paramylon from Euglena is well known, for example, E. Ziegler, “Die naturlichen und kunstlichen Aromen” Heidelberg, Germany, 1982, Chapter 4.3 “Gefriertrocken”, DE 43328329, or JP 2003-529538. It is described in the publication.

(Processed paramylon)
Examples of processed products of paramylon include water-soluble paramylon, sulfated paramylon, etc. obtained by chemically or physically treating paramylon by various known methods, and paramylon derivatives.

Examples of processed products of paramylon include amorphous paramylon.
Amorphous paramylon is a substance obtained by amorphizing crystalline paramylon derived from Euglena.
Amorphous paramylon has a relative crystallinity of 1 to 20% with respect to crystalline paramylon produced by a method known from Euglena.
However, this relative crystallinity is obtained by the method described in JP-A-2011-184592.
That is, amorphous paramylon and paramylon were each pulverized at a vibration frequency of 20 times / second for 5 minutes with a pulverizer (Retsh Ball Mill MM400), and then used with an X-ray diffractometer (Spectris H'PertPRO). Scanning is performed at a voltage of 45 KV and a tube current of 40 mA in a range of 2θ of 5 ° to 30 °, and diffraction peaks Pc and Pa in the vicinity of 2θ = 20 ° of paramylon and amorphous paramylon are obtained.
Using the values of Pc and Pa, the relative crystallinity of amorphous paramylon is
Relative crystallinity of amorphous paramylon = Pa / Pc × 100 (%)
Calculated by

Amorphous paramylon is prepared according to the method described in JP-A-2011-184592 by treating the crystalline paramylon powder with an alkali followed by neutralization with an acid, and thereafter washing and drying steps and drying.
Other processed products of paramylon include water-soluble paramylon, sulfated paramylon, and the like obtained by chemically or physically treating paramylon by various known methods, and paramylon derivatives.

<Food factor sensing-related gene expression promoter>
The food factor sensing related gene expression promoter according to the present embodiment is a food factor sensing related gene expression promoter (food factor sensing related gene expression promoter) containing an Euglena-derived substance as an active ingredient.
The “Euglena-derived substance” includes Euglena algal cells such as Euglena live cells and dried algal cells of Euglena, as well as aqueous solvent extracts of Euglena, processed products of Euglena algal bodies, processed products of paramylon and paramylon, and the like.

  “Food factor sensing-related gene expression promoter” refers to an agent that promotes the expression of food factor sensing-related genes, and is synonymous with “food factor sensing-related gene expression increasing agent”. The increase in gene amount can be evaluated by, for example, measuring the gene expression level by a known method such as a PCR method.

  The food factor sensing-related gene expression promoting agent of the present embodiment promotes the expression of food factor sensing-related genes involved in food factor sensing (sensing), thereby allowing food factor sensing-related proteins (food factor acceptance). It is possible to promote the expression of the body).

<Food factor function enhancer>
The food factor sensing-related gene expression promoter according to the present embodiment contains an Euglena-derived substance as an active ingredient, and can also be used as a food factor function enhancer for enhancing the food factor function.

  “Enhancing the food factor function” means that the food factor function in the living body is enhanced as compared with the state before ingesting the Euglena-derived substance that is the active ingredient of the food factor function enhancer of the present embodiment.

  The food factor sensing-related gene expression promoting agent of the present embodiment promotes the expression of a food factor sensing-related gene in a living body and promotes the expression of a protein (food factor receptor) related to food factor sensing. Acts to enhance the food factor function.

<Food additive for enhancing food factor function>
The food factor function enhancer of the present embodiment contains a Euglena-derived substance as an active ingredient, and is also used as a food additive for food factor function enhancement for enhancing food factor function (food additive for food factor function enhancement). be able to.
Since Euglena-derived substances can enhance food factor function, they can be added to foods such as foods for specified health use, nutritional functional foods, functional labeling foods, hospital patient foods, and supplements. The food factor function of the contained food factor can be enhanced.

<Use>
The food factor sensing-related gene expression promoter and food factor function enhancer containing the Euglena-derived substance according to the present embodiment as an active ingredient are configured as food compositions such as health foods, pharmaceutical compositions, and food factor sensing functions Is administered to living organisms with reduced levels.

  Euglena-derived substances such as Euglena alga and paramylon can be ingested as a food and have no side effects, and therefore can be administered even before receiving a definitive diagnosis of a specific disease.

  The food factor sensing-related gene expression promoter containing the Euglena-derived substance of the present embodiment as an active ingredient, the food factor function enhancer, food, due to some reason such as lifestyle, aging, eating habits, lack of exercise, etc. It can be administered continuously for a long period of time to a person who is likely to have a reduced factor sensing function.

  The food factor sensing-related gene expression promoter containing the Euglena-derived substance according to the present embodiment as an active ingredient, and the subject to which the food factor function enhancer is administered are not limited to those in the above state or animals other than humans. Absent.

In addition, a food factor sensing-related gene expression promoter containing a Euglena-derived substance as an active ingredient or a food factor function enhancer may be administered to a human being 40 years of age or older, particularly to an elderly person over 60 or 65 years old. it can.
Although humans over 40 years old, especially elderly people over 60 or 65 years old, have a tendency to decrease the food factor sensing function due to aging, food factor sensing-related gene expression promoting action and foods provided by Euglena-derived substances The sensing function of the food factor can be improved by the factor function enhancing action.

  According to the food factor sensing-related gene expression promoter of the present embodiment, the food factor sensing function in the living body can be improved, so that the food factor can be added to and combined with a food containing a specific food factor. Applications are expected to improve the food factor function of

  Moreover, the food factor sensing-related gene expression promoter containing the Euglena-derived substance according to the present embodiment as an active ingredient is added with a pharmacologically acceptable additive, and a composition such as a pharmaceutical composition, a food composition, etc. Can be used as

(Food composition)
The Euglena-derived substance of the present embodiment can also be used for food.
The food composition for promoting gene expression related to food factor sensing according to the present embodiment is used in various foods in the field of food, using an effective amount of Euglena-derived substance capable of effectively exerting an effect of promoting gene expression related to food factor sensing as a food material. By blending, a food composition having the action can be provided.
Moreover, it is also possible to provide a food composition by combining an Euglena-derived substance with a specified health food, a nutritive function food, a functional indication food, a hospital patient food, a supplement and the like.
Examples of the food composition include seasonings, processed meat products, processed agricultural products, beverages (soft drinks, alcoholic beverages, carbonated beverages, milk beverages, fruit juice beverages, tea, coffee, nutritional drinks, etc.), powdered beverages (powder) Juices, powdered soups, etc., concentrated beverages, confectionery (candy (nodice), cookies, biscuits, gums, gummi, chocolate, etc.), bread, cereals, etc. can be mentioned. Moreover, in the case of food for specified health use, nutritional functional food, functional indication food, etc., it may be in the form of capsule, troche, syrup, granule, powder or the like.

Here, food for specified health use is a food containing health functional ingredients that affect physiological functions, etc., and can be used to indicate that it is suitable for specific health use with the permission of the Commissioner of the Consumer Affairs Agency. is there.
The nutritional functional food is a food used for supplementing nutritional components (vitamins and minerals) and displays the function of the nutritional components. In order to sell it as a nutritionally functional food, it is necessary for the amount of nutritional component contained in the daily intake standard amount to be within the defined upper limit value and lower limit value, and not only the display of nutritional function but also alert display etc. You also need to.
In addition, the functional labeling food is a food which displays scientific basis-based functionality at the responsibility of the business operator. Information on the basis of safety and functionality etc. was sent to the Secretary General of the Consumer Agency before sales.

  In addition to the Euglena-derived substance, one or more components that can be used in a normal food composition can be freely selected and blended in the food composition according to this embodiment. For example, all additives that can be usually used in the food field, such as various seasonings, preservatives, emulsifiers, stabilizers, fragrances, colorants, preservatives, and pH adjusters can be contained.

  In addition to the Euglena-derived substance, one or more other substances known to have a food factor sensing-related gene expression promoting effect can be added to the food composition according to this embodiment.

(Pharmaceutical composition)
The food factor sensing related gene expression promoter containing the Euglena-derived substance according to the present embodiment as an active ingredient can be used as a pharmaceutical composition.
In the pharmaceutical field, the food factor sensing-related gene expression promoter of the present embodiment comprises a pharmaceutically acceptable carrier or additive together with an amount of a Euglena-derived substance that can effectively exert a food factor sensing-related gene expression promoting effect. The pharmaceutical composition which has the said effect | action is provided by mix | blending. The pharmaceutical composition may be a pharmaceutical or a quasi drug.
The pharmaceutical composition may be applied internally or externally. Therefore, the pharmaceutical composition is used in the form of an internal preparation, intravenous injection, subcutaneous injection, intradermal injection, injection such as intramuscular injection and / or intraperitoneal injection, transmucosal application, transdermal application and the like. be able to.
The dosage form of the pharmaceutical composition can be appropriately set depending on the form of application, for example, solid preparations such as tablets, granules, capsules, powders, powders, liquid preparations such as liquids and suspensions, Semi-solid preparations such as ointments or gels are mentioned.

In the pharmaceutical composition according to this embodiment, one or more pharmaceutically acceptable additives can be freely selected and contained.
For example, when the pharmaceutical composition according to the present embodiment is applied to an oral preparation, for example, an excipient, a binder, a disintegrant, a surfactant, a preservative, a coloring agent, a corrigent, a fragrance, a stabilizer, an antiseptic, for example. All additives that can be usually used in the field of pharmaceutical preparations, such as agents and antioxidants, can be contained. In addition, a sustained-release preparation can be obtained using a drug delivery system (DDS).

  In addition to the Euglena-derived substance, one or more other substances known to have a food factor sensing-related gene expression promoting effect can be added to the pharmaceutical composition according to the present embodiment.

<Dosage and administration>
As the usage of the food factor sensing-related gene expression promoter of this embodiment, for example, it may be orally administered by powder, capsule, tablet, granule, liquid or syrup.
What is necessary is just to select suitably the dosage amount and administration form of the food factor sensing related gene expression promoter of this embodiment according to a subject, a disease state, its progress, and other conditions. For example, when euglena is selected as an euglena-derived substance and orally administered for the purpose of obtaining food factor sensing related gene expression promotion effect to human (adult), generally euglena is applied at 1 dry dw per day. ˜5000 mg, preferably 100 to 3000 mg, more preferably about 500 to 2000 mg, about 1 to 2 times a day (morning and evening), preferably at a rate of 5 times a week or more .

Hereinafter, the present invention will be specifically described based on specific examples, but the present invention is not limited to these.
In the following tests, using euglena powder (example 1) and paramylon powder (example 2) as euglena-derived substances, clarifying the influence of continuous intake of euglena or paramylon on the expression of food factor sensing related genes It is aimed.

Example 1
As a food factor sensing-related gene expression promoter, Euglena gracilis powder (Euglena algal, manufactured by Euglena Co., Ltd.) which is an Euglena-derived substance was used.

Example 2
As a food factor sensing-related gene expression promoter, paramylon powder (manufactured by Euglena Co., Ltd.), which is an Euglena-derived substance, was used.

<Examination of the effect of continuous intake of Euglena-derived substances on the expression level of food factor sensing-related genes>
(Test method)
-Feed preparation A CE-2 meal (purchased from Nippon Claire) was mixed to contain 2% Euglena powder to prepare a Euglena meal. Similarly, a paramylon meal was prepared by mixing 2% paramylon powder in the CE-2 meal. Tables 5 to 7 show the nutritional components of the CE-2 diet.

Feeding test 12-week-old C57BL / 6J male mice were divided into 3 groups so that the average body weights were equal to each other, and they were designated as a control group (C), a Euglena group (E), and a paramylon group (P) (n = 6). The control group was fed with CE-2 (normal diet), the Euglena group was fed with 2% Euglena-containing CE-2 (Euglena diet), and the paramylon group was fed with 2% paramylon-containing CE-2 (paramylon diet) (Table). 8). After the 4-week feeding period, quadriceps muscle and liver were collected from the mice.

-Gene expression analysis After extracting RNA from the collected organ using Trireagent (Cosmo Bio), a mixture of RNA for 6 mice in each group (n = 1) was used as an analysis sample, and a microarray (Agilent SurePrint G3 Mouse) Gene expression was measured using GE microarray kit 8 × 60 K v2).

(Result of Test 1)
The results in the liver are shown in FIGS. 1A-1D and FIG. 2, and the results in the quadriceps femoris muscle in FIGS. 3A-3C and FIG.
Liver In the liver, in the Euglena group, Adora 2a (SEQ ID NO: 1), Adora 3 (SEQ ID NO: 2), Adra 1a (SEQ ID NO: 3), Bcl 2 (SEQ ID NO: 4), Ccr 2 (SEQ ID NO: 5), Ccr 5 (SEQ ID NO: 6), Ccr6 (SEQ ID NO: 7), Cd36 (SEQ ID NO: 8), Ffar1 (SEQ ID NO: 9), Gabbr1 (SEQ ID NO: 10), Glra4 (SEQ ID NO: 11), Hic2 (SEQ ID NO: 12), IL1a (SEQ ID NO: 13), IL1b (SEQ ID NO: 14), IL6 (SEQ ID NO: 15), IL16 (SEQ ID NO: 16), Ppara (SEQ ID NO: 17), Tlr1 (SEQ ID NO: 18), Tlr3 (SEQ ID NO: 19), Tlr5 (SEQ ID NO: 20), Tlr6 ( 21 genes of SEQ ID NO: 21 were increased by 1.5 times or more compared to the control group.
In the liver, in the paramylon group, Adora3 (SEQ ID NO: 2), Adra1a (SEQ ID NO: 3), Ager (SEQ ID NO: 22), Bcl2 (SEQ ID NO: 4), Ccr5 (SEQ ID NO: 6), Cd4 (SEQ ID NO: 23), 13 of Esrrb (SEQ ID NO: 24), IL1b (SEQ ID NO: 14), IL1r1 (SEQ ID NO: 25), Ltb4r1 (SEQ ID NO: 26), Tlr6 (SEQ ID NO: 21), Olr1 (SEQ ID NO: 27), Stx3 (SEQ ID NO: 28) The gene increased 1.5 times or more compared with the control group.
In the liver, six genes of Adra1a (SEQ ID NO: 3), Adora3 (SEQ ID NO: 2), Bcl2 (SEQ ID NO: 4), Ccr5 (SEQ ID NO: 6), IL1b (SEQ ID NO: 14), Tlr6 (SEQ ID NO: 21) In both the euglena group and the paramylon group, the increase was 1.5 times or more compared to the control group.

  Therefore, Euglena and paramylon as Euglena-derived substances in the liver have the effect of increasing food factor sensing-related genes. Therefore, Euglena-derived substances are used as food factor sensing-related gene expression promoters and food factor sensing-related gene expression promoting food compositions. It turned out that it can be used as a thing.

In quadriceps femoris, in the Euglena group, Bcl2l1 (SEQ ID NO: 29), Ccr6 (SEQ ID NO: 7), Esr1 (SEQ ID NO: 30), Esrrb (SEQ ID NO: 24), Ffar3 (SEQ ID NO: 31), Grlb 11 genes (SEQ ID NO: 32), Ppara (SEQ ID NO: 17), Rxra (SEQ ID NO: 33), IL6ra (SEQ ID NO: 34), Tlr6 (SEQ ID NO: 21), and Vdr (SEQ ID NO: 35) are compared with the control group. It increased by more than 1.5 times.
In the quadriceps muscle, in the paramylon group, Bcl2l1 (SEQ ID NO: 29), Gabbr1 (SEQ ID NO: 10), Gpr84 (SEQ ID NO: 36), IL6ra (SEQ ID NO: 33), IL16 (SEQ ID NO: 16), Sirt1 (SEQ ID NO: 16) 37) and 7 genes of Tlr4 (SEQ ID NO: 38) were increased by 1.5 times or more compared to the control group.
In quadriceps muscle, Bcl2l1 (SEQ ID NO: 29) and IL6ra (SEQ ID NO: 33) 2 genes increased 1.5 times or more in both Euglena and Paramylon groups compared to the control group. It was.

  Therefore, Euglena and paramylon as Euglena-derived substances have the effect of increasing food factor sensing-related genes in quadriceps muscles. Therefore, Euglena-derived substances are used as food factor sensing-related gene expression promoters and food factor sensing-related gene expression promoters. It turned out that it can be used as a food composition.

(The food factor function enhancement effect of Euglena derived substances)
Since Euglena-derived substances promote the expression of food factor sensing-related genes, it is possible to enhance the food factor function as shown in FIGS.
Specifically, as shown in FIG. 5, the intake of Euglena promotes the expression of food factor sensing related genes Ppara, (SEQ ID NO: 17) Vdr (SEQ ID NO: 33), Ffar 1 (SEQ ID NO: 9) Can improve lipid metabolism, which is a food factor function of unsaturated fatty acids, bone protective action, which is a food factor function of vitamin D, and anti-diabetic action, which is a food factor function of long chain fatty acids. is assumed.

  In addition, as shown in FIG. 6, by taking paramylon, the expression of food factor sensing-related genes Sirt1 (SEQ ID NO: 37), Gabbr1 (SEQ ID NO: 10), Adra1a (SEQ ID NO: 3) can be promoted. Therefore, it is assumed that the anti-aging action, which is a food factor function of flavonoids, the blood pressure lowering action, which is a food factor function of GABA, and the adrenaline-like action, which is a food factor function of synephrine, are expected to be enhanced.

<Test 2 Examination of the influence of continuous intake of euglena-derived substances on the expression of soybean isoflavone sensor related genes>
(Test method)
-Feed preparation A CE-2 meal (purchased from Nippon Claire) was mixed to contain 2% Euglena powder to prepare a Euglena meal.

Feeding test 12-week-old C57BL / 6J male mice were divided into 3 groups so that the average body weights were equal to each other, and they were designated as a control group (C), a Euglena group (E), and a paramylon group (P) (n = 6). The control group was fed with CE-2 (normal diet), and the Euglena group was fed with CE-2 (euglena diet) containing 2% Euglena. After a 4-week feeding period, quadriceps muscles were collected from the mice.

-Gene expression analysis Total RNA was collect | recovered from the quadriceps muscle, cDNA was synthesize | combined, and each gene expression level was measured by real-time PCR. The expression level of each gene was corrected using β-actin as an internal standard gene.

(Result of Test 2)
The measurement results of the expression levels of Esr1 (SEQ ID NO: 30) and Esrrb (SEQ ID NO: 24) in the quadriceps muscle are shown in FIG. 7, and the measurement results of the Ctsd (SEQ ID NO: 39) expression level are shown in FIG.

  As a result of measuring the expression level of the gene fluctuating in quadriceps muscle by real-time PCR, it was confirmed that Esr1 (SEQ ID NO: 30) known as a soybean isoflavone sensor and its related gene Esrrb (SEQ ID NO: 24) increased in expression ( FIG. 7). Moreover, as a result of measuring the expression level of Ctsd (SEQ ID NO: 39), the expression of which is induced by the activation of Esr1 (SEQ ID NO: 30), by real-time PCR, an increase in the expression level of Ctsd (SEQ ID NO: 39) is caused by ingestion of Euglena. It was confirmed (FIG. 8).

  Therefore, by taking Euglena, the expression of Esr1 (SEQ ID NO: 30), Esrrb (SEQ ID NO: 24), and Ctsd (SEQ ID NO: 39), which are food factor sensing-related genes, can be promoted. It is assumed that factor function is enhanced.

Claims (13)

Contains an Euglena-derived substance as an active ingredient,
A food composition for promoting food factor sensing related gene expression, which is used to promote expression of a food factor sensing related gene.   The food composition for food factor sensing-related gene expression promotion according to claim 1, wherein the Euglena-derived substance is at least one selected from the group consisting of Euglena or Paramylon. The Euglena-derived substance is Euglena,
Adora 2a (SEQ ID NO: 1), Adora 3 (SEQ ID NO: 2), Adra 1a (SEQ ID NO: 3), Bcl 2 (SEQ ID NO: 4), Ccr 2 (SEQ ID NO: 5), Ccr 5 (SEQ ID NO: 6), Ccr 6 (SEQ ID NO: 7) in the Liver , Cd36 (SEQ ID NO: 8), Ffar1 (SEQ ID NO: 9), Gabbr1 (SEQ ID NO: 10), Glra4 (SEQ ID NO: 11), Hic2 (SEQ ID NO: 12), IL1a (SEQ ID NO: 13), IL1b (SEQ ID NO: 14), It consists of IL6 (SEQ ID NO: 15), IL16 (SEQ ID NO: 16), Ppara (SEQ ID NO: 17), Tlr1 (SEQ ID NO: 18), Tlr3 (SEQ ID NO: 19), Tlr5 (SEQ ID NO: 20) and Tlr6 (SEQ ID NO: 21) The food composition for promoting expression of food factor sensing-related genes according to claim 1 or 2, wherein the food composition is used to increase the expression level of one or more genes selected from the group. The Euglena-derived substance is paramylon,
Adora3 (SEQ ID NO: 2), Adra1a (SEQ ID NO: 3), Ager (SEQ ID NO: 22), Bcl2 (SEQ ID NO: 4), Ccr5 (SEQ ID NO: 6), Cd4 (SEQ ID NO: 23), Esrrb (SEQ ID NO: 24) in the liver , IL1b (SEQ ID NO: 14), IL1r1 (SEQ ID NO: 25), Ltb4r1 (SEQ ID NO: 26), Tlr6 (SEQ ID NO: 21), Olr1 (SEQ ID NO: 27), and Stx3 (SEQ ID NO: 28). The food composition for promoting the expression of food factor sensing-related genes according to claim 1 or 2, wherein the food composition is used for increasing the expression level of the above genes. The Euglena-derived substance is Euglena,
Bcl2l1 (SEQ ID NO: 29), Ccr6 (SEQ ID NO: 7), Esr1 (SEQ ID NO: 30), Esrrb (SEQ ID NO: 24), Ffar3 (SEQ ID NO: 31), Glrb (SEQ ID NO: 32), Ppara (SEQ ID NO: 17) in muscle , Rxra (SEQ ID NO: 33), IL6ra (SEQ ID NO: 34), Tlr6 (SEQ ID NO: 21) and Vdr (SEQ ID NO: 35) to be used to increase the expression level of one or more genes selected from the group consisting of A food composition for promoting food factor sensing related gene expression according to claim 1 or 2, characterized in that The Euglena-derived substance is paramylon,
Bcl2l1 (SEQ ID NO: 29), Gabbr1 (SEQ ID NO: 10), Gpr84 (SEQ ID NO: 36), IL6ra (SEQ ID NO: 33), IL16 (SEQ ID NO: 16), Sirt1 (SEQ ID NO: 37) and Tlr4 (SEQ ID NO: 38) in muscle The food composition for promoting expression of food factor sensing-related genes according to claim 1 or 2, wherein the food composition is used to increase the expression level of one or more genes selected from the group consisting of: Contains Euglena-derived substances as active ingredients,
A food factor sensing-related gene expression promoter characterized by being used for promoting the expression of a food factor sensing-related gene.   The food factor sensing-related gene expression promoter according to claim 7, wherein the Euglena-derived substance is one or more selected from the group consisting of Euglena or Paramylon. Contains an Euglena-derived substance as an active ingredient,
A food composition for enhancing food factor function, which is used to enhance food factor function. Contains an Euglena-derived substance as an active ingredient,
A food factor function enhancer, which is used to enhance food factor function. Contains an Euglena-derived substance as an active ingredient,
A food additive for enhancing food factor function, characterized in that it is used to enhance food factor function. Contains Euglena as an active ingredient,
A food composition for enhancing food factor function characterized in that it is used to enhance the food factor function of soy isoflavone. Contains Euglena as an active ingredient,
A food additive for enhancing food factor function, characterized in that it is used to enhance the food factor function of soy isoflavone.

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