JP2019088335A5 - - Google Patents

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JP2019088335A5
JP2019088335A5 JP2019053209A JP2019053209A JP2019088335A5 JP 2019088335 A5 JP2019088335 A5 JP 2019088335A5 JP 2019053209 A JP2019053209 A JP 2019053209A JP 2019053209 A JP2019053209 A JP 2019053209A JP 2019088335 A5 JP2019088335 A5 JP 2019088335A5
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cell
embedding member
cell treatment
treatment device
embedding
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JP2019088335A (en
JP7090337B2 (en
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Claims (21)

細胞を処理する細胞処理器具と、
前記細胞処理器具を包埋する包埋部材と、
前記包埋部材の外と前記包埋部材内の前記細胞処理器具とを連通させる連通送液路と、
前記連通送液路内の液体を送液するための、前記包埋部材の外部に配置される駆動部と、
を備え、
前記包埋部材内に、前記駆動部から駆動力が伝達される従動部が設けられており、当該従動部が、前記連通送液路に接続されており、
前記細胞処理器具で前記細胞が培養される、細胞処理装置。
A cell treatment device for treating cells,
An embedding member for embedding the cell treatment instrument,
A communication liquid passage for communicating the outside of the embedding member and the cell treatment instrument inside the embedding member,
A driving unit arranged outside the embedding member for feeding the liquid in the communication liquid feeding path;
Equipped with
In the embedding member, a driven portion to which a driving force is transmitted from the driving portion is provided, and the driven portion is connected to the communication liquid feeding path,
A cell treatment device, wherein the cells are cultured in the cell treatment instrument.
前記包埋部材がガラス又は樹脂からなる、請求項1に記載の細胞処理装置。   The cell treatment device according to claim 1, wherein the embedding member is made of glass or resin. 前記包埋部材が金属からなる、請求項1に記載の細胞処理装置。   The cell treatment device according to claim 1, wherein the embedding member is made of metal. 前記駆動部が、前記包埋部材の外壁に接続される、請求項1に記載の細胞処理装置。   The cell treatment device according to claim 1, wherein the drive unit is connected to an outer wall of the embedding member. 細胞を処理する細胞処理器具と、
前記細胞処理器具を包埋する包埋部材と、
前記包埋部材の外と前記包埋部材内の前記細胞処理器具とを連通させる連通送液路と、
を備え、
前記細胞処理器具が、血液から単核球を分離する、前記包埋部材の内部に配置された単核球分離部を備える、細胞処理装置。
A cell treatment device for treating cells,
An embedding member for embedding the cell treatment instrument,
A communication liquid passage for communicating the outside of the embedding member and the cell treatment instrument inside the embedding member,
Equipped with
The cell treatment device, wherein the cell treatment instrument includes a mononuclear cell separation unit arranged inside the embedding member, for separating mononuclear cells from blood.
前記包埋部材の外部に配置される、血液及び血液細胞の少なくともいずれかを保存する血液保存部をさらに備え、
前記連通送液路が、前記血液保存部と前記単核球分離部を連通させる血液送液路を備える、請求項5に記載の細胞処理装置。
Further comprising a blood storage unit arranged outside the embedding member for storing at least one of blood and blood cells,
The cell processing apparatus according to claim 5, wherein the communication liquid supply path includes a blood liquid supply path that connects the blood storage unit and the mononuclear cell separation unit.
細胞を処理する細胞処理器具と、
前記細胞処理器具を包埋する包埋部材と、
前記包埋部材の外と前記包埋部材内の前記細胞処理器具とを連通させる連通送液路と、
を備え、
前記細胞処理器具が、細胞を単離する、前記包埋部材の内部に配置されたフィルターを備える、細胞処理装置。
A cell treatment device for treating cells,
An embedding member for embedding the cell treatment instrument,
A communication liquid passage for communicating the outside of the embedding member and the cell treatment instrument inside the embedding member,
Equipped with
The cell treatment device, wherein the cell treatment instrument includes a filter arranged inside the embedding member for isolating cells.
細胞を処理する細胞処理器具と、
前記細胞処理器具を包埋する包埋部材と、
前記包埋部材の外と前記包埋部材内の前記細胞処理器具とを連通させる連通送液路と、
を備え、
前記細胞処理器具が、前記包埋部材の内部に配置された単核球精製フィルターを備える、細胞処理装置。
A cell treatment device for treating cells,
An embedding member for embedding the cell treatment instrument,
A communication liquid passage for communicating the outside of the embedding member and the cell treatment instrument inside the embedding member,
Equipped with
The cell treatment device, wherein the cell treatment instrument comprises a mononuclear cell purification filter arranged inside the embedding member.
細胞を処理する細胞処理器具と、
前記細胞処理器具を包埋する包埋部材と、
前記包埋部材の外と前記包埋部材内の前記細胞処理器具とを連通させる連通送液路と、
を備え、
前記細胞処理器具が、
血液から単核球を分離する、前記包埋部材の内部に配置された単核球分離部と、
前記包埋部材の内部に配置された単核球精製フィルターと、
前記単核球分離部と前記単核球精製フィルターを連通させる、前記包埋部材の内部に配置された単核球送液路と、
を備える、細胞処理装置。
A cell treatment device for treating cells,
An embedding member for embedding the cell treatment instrument,
A communication liquid passage that connects the outside of the embedding member and the cell treatment instrument inside the embedding member,
Equipped with
The cell treatment instrument,
Separating mononuclear cells from blood, a mononuclear cell separating portion arranged inside the embedding member,
A mononuclear cell purification filter disposed inside the embedding member,
Connecting the mononuclear cell separation unit and the mononuclear cell purification filter, a mononuclear cell liquid supply passage arranged inside the embedding member,
A cell processing device comprising:
前記単核球送液路内の液体を送液するための、前記包埋部材の外部に配置される駆動部をさらに備える、請求項9に記載の細胞処理装置。   The cell treatment device according to claim 9, further comprising a driving unit arranged outside the embedding member for feeding the liquid in the mononuclear cell liquid feeding path. 前記駆動部が、前記包埋部材の外壁に接続される、請求項10に記載の細胞処理装置。   The cell processing device according to claim 10, wherein the drive unit is connected to an outer wall of the embedding member. 前記包埋部材内に、前記駆動部から駆動力が伝達される従動部が設けられており、当該従動部が、前記単核球送液路に接続されている、請求項10又は11に記載の細胞処理装置。   The driven part to which the driving force is transmitted from the drive part is provided in the embedding member, and the driven part is connected to the mononuclear cell liquid feeding path. Cell processing equipment. 細胞を処理する細胞処理器具と、
前記細胞処理器具を包埋する包埋部材と、
前記包埋部材の外と前記包埋部材内の前記細胞処理器具とを連通させる連通送液路と、
を備え、
前記細胞処理器具が、細胞に誘導因子を導入して誘導因子導入細胞を作製する、前記包埋部材の内部に配置された因子導入部を備える、細胞処理装置。
A cell treatment device for treating cells,
An embedding member for embedding the cell treatment instrument,
A communication liquid passage for communicating the outside of the embedding member and the cell treatment instrument inside the embedding member,
Equipped with
The cell treatment device, wherein the cell treatment instrument includes a factor-introducing section disposed inside the embedding member, for producing an inducer-introduced cell by introducing an inducer into the cell.
前記包埋部材の外部に配置される、誘導因子を保存する因子保存部をさらに備え、
前記連通送液路が、前記因子保存部と前記因子導入部を連通させる因子送液路を備える
、請求項13に記載の細胞処理装置。
Further comprising a factor storage unit disposed outside the embedding member, for storing an induction factor,
The cell processing device according to claim 13, wherein the communication liquid supply path includes a factor liquid supply path that connects the factor storage unit and the factor introduction unit.
前記因子導入部において、RNAリポフェクションにより、前記細胞に前記誘導因子が導入される、請求項13又は14に記載の細胞処理装置。   The cell treatment device according to claim 13 or 14, wherein the induction factor is introduced into the cell by RNA lipofection in the factor introduction part. 前記誘導因子がDNA、RNA、又はタンパク質である、請求項15に記載の細胞処理装置。   The cell treatment device according to claim 15, wherein the inducer is DNA, RNA, or protein. 前記誘導因子がベクターに組み込まれている、請求項13又は14に記載の細胞処理装置。 The cell treatment device according to claim 13 or 14, wherein the inducer is incorporated in a vector. 前記ベクターがセンダイウイルスベクターである、請求項17に記載の細胞処理装置。   The cell treatment device according to claim 17, wherein the vector is a Sendai virus vector. 前記細胞処理器具が、誘導因子を導入した誘導因子導入細胞を培養する、前記包埋部材の内部に配置された培養器を備える、請求項1から3及び4のいずれか1項に記載の細胞処理装置。 The cell according to any one of claims 1 to 3 and 4, wherein the cell treatment device includes an incubator arranged inside the embedding member, for culturing an inducer-introduced cell into which an inducer has been introduced. Processing equipment. 前記細胞処理器具が、樹立された幹細胞からなる複数の細胞塊を拡大培養する、前記包埋部材の内部に配置された拡大培養器を備える、請求項1から3及び4のいずれか1項に記載の細胞処理装置。   5. The cell processing instrument according to claim 1, further comprising an expansion incubator arranged inside the embedding member, for expanding and culturing a plurality of cell clusters composed of established stem cells. The described cell treatment device. 前記細胞処理器具が、細胞周囲の溶液を置換する溶液置換器を備える、請求項1から3及び4のいずれか1項に記載の細胞処理装置。   The cell treatment device according to any one of claims 1 to 3 and 4, wherein the cell treatment instrument includes a solution displacement device that displaces a solution around cells.
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