JP2019083816A - Inspection device - Google Patents

Abstract

To provide an inspection device for making a liquid leak by breaking a container storing a liquid such as an expansion liquid or sample solution, in a housing storing a chromatography carrier, and bringing the liquid into contact with the carrier, capable of making the liquid efficiently leak, in one or more embodiments of the invention.SOLUTION: An inspection device 1 of one or more embodiments comprises a housing 10 for storing a chromatography carrier 30. The housing 10 comprises: a support part 14 for supporting a container 40; and a blade 13 for cutting the container 40. When a length of the container 40 is M, and a length of a notch 43 formed on the container 40 when the container 40 is supported to the support part 14 and moved to the blade 13 is D, in a state in which the container is supported to the support part 14, D/M is 0.2 or more.SELECTED DRAWING: Figure 3B

Description

  The present invention relates to a test device used for chromatography.

  Nucleic acid chromatography is a technology for developing and capturing and detecting a substance to be detected having a nucleic acid tag attached thereto that hybridizes with the nucleic acid immobilized on the carrier on a chromatography carrier on which nucleic acid is immobilized. A plurality of detection targets contained in one sample are fixed by fixing nucleic acids having different base sequences at different positions on the chromatography carrier and adding a nucleic acid tag specific to the nucleic acids to a plurality of detection targets. It is possible to detect the substance on one carrier. In addition to nucleic acid chromatography, there is also a chromatography technology that captures a substance to be detected on a chromatography carrier using an antigen-antibody combination that can form a specific bond or a ligand-receptor combination. It is widely used in the fields of molecular biology testing and genetic testing for medical and food testing.

  In the above-described chromatography, various liquids such as a sample liquid containing a substance to be detected and a developing liquid are used. When these liquids are removed from the container with an instrument such as a pipette and applied to a chromatography carrier, the liquids may scatter in the test environment, resulting in contamination that causes false positives. In addition, opening the container in the inspection environment may contaminate the liquid in the container.

  With a liquid such as a sample liquid or a developing liquid used for chromatography contained in a container, a part of the container is broken to leak the liquid and brought into contact with the chromatography carrier without using an instrument such as a pipette Devices are being developed.

For example, a device for detecting an analyte in an affinity bioassay disclosed in Patent Document 1 is
At least one transparent wall,
At least one test recess adapted to receive at least one test strip and arranged so that the test strip is visible through the transparent wall;
At least one cavity adapted to receive a container containing a fluid sample, said cavity being in fluid communication with said at least one test recess;
The container is pierced when the container is inserted into the cavity, and is disposed corresponding to the at least one cavity such that at least a portion of the sample is expelled from the container to reach the test recess And drilling means.

In addition, the completely sealed target nucleic acid amplification product high-speed inspection device disclosed in Patent Document 2 is
Including inner structure and outer box,
The internal structure includes a fixed box part and a pedestal part,
The fixed box part has corresponding holes, which are used to hold the washing solution pack and the PCR tube containing the amplification product,
The pedestal portion includes a cleaning solution storage unit having a liquid pack piercing needle, an amplification product storage unit having a cutter, a sealing partition, a glass fiber paper, and a test strip sealing portion having a test strip and a transparent window,
The outer box is characterized by including a lever cover, a fixed box pressure portion, a washing solution pack pressure portion and a transparent window.

JP-A-2015-512250 Japanese Patent Publication No. 2010-500009

  A device in which a part of a container containing the liquid is broken to leak the liquid and brought into contact with the chromatography carrier in a housing containing the chromatography carrier is described in Patent Documents 1 and 2, and the like.

  However, when destroying the container containing the liquid, the inventor found a problem that the inside of the container becomes negative pressure when a part of the liquid in the container leaks, and no further leakage of the liquid occurs. .

An inspection device according to an embodiment of the present invention is
It has an enclosure for accommodating the chromatography carrier,
The housing is
A support for supporting a container containing a liquid used for chromatography;
And a blade for cutting the container supported by the support, and for leaking liquid from the container to a position contacting the carrier.
From the first position where one end of the container faces the cutting edge of the blade to the second position where the container is incised by the blade from the side of the end of the container with the support portion supporting the container , And a container guiding unit for guiding the container is formed;
Let M be the length of the container along the guiding direction, which is the direction of being guided by the container guiding portion in the state of being supported by the support portion,
Assuming that the maximum length of the cut formed in the container along the guiding direction is D when the container is supported by the support portion and guided from the first position to the second position,
It is characterized in that D / M is 0.2 or more.

  In the inspection device according to this embodiment, when the container is supported by the support portion and guided from the first position to the second position, the length M of the container in the guiding direction is 0.2 M or more (= D The cut of the length is formed. For this reason, the container of the device for inspection according to this embodiment, which accommodates the liquid to the depth less than D corresponding to the maximum length of the cut on the side of one end of the container and uses the remaining portion as the gas phase When supported by the support portion of the body and guided from the first position to the second position, the blade forms a cut in the container up to the portion surrounding the gas phase in the container. At this time, air is supplied to the gas phase from the outside of the container through the portion connecting the gas phase and the outside of the container of the incision formed in the container, so when the liquid leaks from the container, the gas phase of the container has a negative pressure And the liquid can leak out of the container efficiently. In order to achieve this effect, the liquid can be contained in the container at a depth of less than D, ie, at least less than 0.2M.

An inspection device according to another embodiment of the present invention is
It has an enclosure for accommodating the chromatography carrier,
The housing is
A support for supporting a container containing a liquid used for chromatography;
And a blade for cutting the container supported by the support, and for leaking liquid from the container to a position contacting the carrier.
From the first position where one end of the container faces the cutting edge of the blade to the second position where the container is incised by the blade from the side of the end of the container with the support portion supporting the container , And a container guiding unit for guiding the container is formed;
The distance along the vertical direction from the end of the container when the container is supported by the support portion to be at the first position to the portion where the liquid level is located when the liquid is contained in the container Assuming that A, and then guiding the container from the first position to the second position, when the maximum length along the vertical direction of the incision formed in the container is B,
It is characterized in that B is larger than A.

  The container containing the liquid used for chromatography together with the gas phase is supported at the first position on the support portion of the case of the inspection device according to this embodiment and guided from the first position to the second position When the container is cut by the blade from the one end of the container to the portion surrounding the gas phase in the container. At this time, air is supplied to the gas phase from the outside of the container through the portion connecting the gas phase and the outside of the container of the incision formed in the container, so when the liquid leaks from the container, the gas phase of the container has a negative pressure And the liquid can leak out of the container efficiently.

In a more preferable aspect of the above-mentioned inspection device,
The blade is a plate-like blade having a blade edge formed on a part of the sides and a base end formed on the other side,
A fixing portion is formed on the inner wall surface of the housing so as to clamp the base end of the blade from both sides in the thickness direction of the blade,
The fixing portion includes a tapered portion formed so that the dimension in the thickness direction of the blade becomes smaller as it approaches the edge of the blade,
When the container is supported by the support portion and guided from the first position to the second position, the tapered portion of the fixing portion is inserted into the notch formed in the container.

  According to this aspect, the tapered portion of the fixing portion is inserted into the notch formed in the container supported by the support portion and guided from the first position to the second position, thereby leaking the liquid from the container. It can be done more efficiently.

In a further preferred aspect of this aspect,
The tapered portion of the fixed portion has an uneven surface that protrudes and / or is recessed in the thickness direction of the blade.

  According to this preferred embodiment, when the tapered portion of the fixed portion is inserted into the cut of the container, a gap is easily formed between the cut of the container and the uneven surface of the tapered portion of the fixed portion. Are more likely to leak out.

Another embodiment of the invention is
A method of performing chromatography using a test device, comprising:
The test device is
Chromatography carrier, and
A housing for containing the carrier;
The housing is
A support for supporting a container containing a liquid used for chromatography;
And a blade for cutting the container supported by the support, and for leaking liquid from the container to a position contacting the carrier.
From the first position where one end of the container faces the cutting edge of the blade to the second position where the container is incised by the blade from the side of the end of the container with the support portion supporting the container , And a container guiding unit for guiding the container is formed;
Supporting the container containing the liquid to be used for chromatography together with the gas phase on the support of the test device so as to be at the first position;
Guiding the container from the first position to the second position after the step 1;
Let A be the distance along the vertical direction from the end of the container supported by the support in step 1 to the liquid level in the container,
Assuming that the maximum length along the vertical direction of the incision formed in the container in step 2 is B,
A method characterized in that B is larger than A.

  According to the method of this embodiment, when the container containing the liquid used for chromatography is supported by the support portion of the case of the test device and guided when moved from the first position to the second position, the blade A cut is made in the container. At this time, air is supplied to the gas phase from the outside of the container through the portion connecting the gas phase and the outside of the container of the incision formed in the container, so when the liquid leaks from the container, the gas phase of the container has a negative pressure The liquid can escape efficiently from the cut of the container.

  In the test device and method according to an embodiment of the present invention, the liquid used for chromatography can be efficiently leaked from the container.

It is an exploded perspective view of a device for inspection concerning an embodiment of the present invention. 1 is a perspective view of an inspection device according to an embodiment of the present invention. It is the perspective view seen from the lower part of the case upper member of the device for inspection which concerns on embodiment of this invention. It is a perspective view of a blade of an inspection device concerning an embodiment of the present invention. In a device for inspection concerning an embodiment of the present invention, it is a cross section schematic diagram of a longitudinal direction in the state where a container was supported by a supporter of a case and it was made to position in the 1st position. In a device for inspection concerning an embodiment of the present invention, a container is supported by a supporter of a case, it is located in the 2nd position, and is a cross section schematic diagram in a longitudinal direction in a state before a liquid leaks from a container. The device for an inspection concerning the embodiment of the present invention WHEREIN: A container is supported by the support part of a housing | casing, and it is a cross-sectional schematic diagram of the longitudinal direction in the state after liquid was leaked from a container. The device for an inspection which concerns on embodiment of this invention WHEREIN: It is a schematic diagram of the end surface of the transversal direction containing one of a container in the state which supported the container by the support part of the housing and it made it position in the 1st position. In the inspection device according to an embodiment of the present invention, the container is supported by the support portion of the housing and is positioned at the second position, and the lateral direction including one of the containers in a state before the liquid leaks from the container FIG. In the inspection device according to the embodiment of the present invention, the container is supported by the support portion of the housing and positioned at the second position, and the lateral direction including one of the containers in a state after the liquid leaks from the container It is a schematic diagram of the end face of the. It is a cross-sectional schematic diagram in the thickness direction of a blade of one embodiment of a fixed part which has a taper part for fixing a blade to a case. It is the cross-sectional schematic diagram in the thickness direction of the blade of the state which pushed in the container to the blade fixed to the fixing | fixed part which has a taper part shown to FIG. 5A until a taper part is inserted in a notch | incision. It is the cross-sectional schematic diagram in the thickness direction of the blade of other embodiment of the fixing | fixed part which has a taper part for fixing a blade to a housing | casing. It is a schematic diagram of the cross section in the II line of the fixing | fixed part shown to FIG. 6A. It is a figure for demonstrating the relationship between the length of the notch | incision which a container is cut and formed in a blade, and the liquid volume of a container. (1) and (2) show the cuts of Example 1, (3) and (4) show the cuts of Comparative Example 1, and (5) and (6) show the cuts of Example 2. It is a figure for demonstrating the relationship between the length of the notch | incision which a container is cut and formed in a blade, and the liquid volume of a container. (1) and (2) show the cuts of Example 3, (3) and (4) show the cuts of Comparative Example 2, and (5) and (6) show the cuts of Example 4. Fig. 8-1 is a continuation of Fig. 8-1, and (7) shows a cut of Example 5. 1 shows the structure of a chromatography carrier that can be used in the present invention. (A) shows a plan view and (B) shows a side view. 2 shows the structures of other chromatography supports that can be used in the present invention. (A) shows a plan view and (B) shows a side view.

  Although specific embodiments of the present invention will be described below with reference to the drawings, the scope of the present invention is not limited to the illustrated embodiments.

<Overview of Inspection Device of First Embodiment>
The inspection device 1 of the first embodiment will be described with reference to FIGS. 1A to 4C.

  The inspection device 1 of the present embodiment includes a housing 10 and a lid 20. The material which comprises the housing | casing 10 and the cover body 20 is not specifically limited, Materials, such as resin and glass, can be used suitably. In the inspection device of the present invention, the lid 20 provided in the inspection device 1 of the present embodiment is not an essential component as described later.

The housing 10 internally defines an internal space 11 for housing the chromatography carrier 30.
The housing 10 also includes a blade 13 disposed in the internal space 11 for cutting the containers 40, 40 and leaking the liquid L from the containers 40, 40 to a position where it contacts the chromatography carrier 30.

  In the illustrated example, the housing 10 is formed by the housing upper member 10A and the housing lower member 10B. The housing lower member 10 </ b> B includes a bottom wall portion 101 of the housing 10 and a side wall portion 102 erected from the periphery of the bottom wall portion 101. The housing upper member 10 </ b> A includes an upper wall portion 103 of the housing 10, a support portion 14 to be described later, and a lid mounting portion 15. In the upper wall portion 103 of the housing upper member 10A, the surface that is the outer surface of the housing 10 when combined with the housing lower member 10B is the upper surface 103a, and the surface that is the inner surface of the housing 10 is the lower surface 103b. Four locking claws 10A1 protruding toward the lower surface 103b are formed on the peripheral edge of the upper wall 103 of the housing upper member 10A, and the outer surface 102a of the side wall 102 of the housing lower member 10B is formed. Four locking recesses 10B1 that engage with the locking claws 10A1 are formed.

  The upper end 102b of the side wall portion 102 of the lower case member 10B and the peripheral portion 103b1 of the lower surface 103b of the upper wall portion 103 of the upper case member 10A engage the upper case member 10A and the lower case member 10B. When they hit each other. Further, on the lower surface 103b of the upper wall portion 103, a protruding portion 103b2 is formed adjacent to the inner peripheral side along the peripheral edge portion 103b1 and protruding from the peripheral edge portion 103b1. When the housing upper member 10A and the housing lower member 10B are engaged, the outer peripheral surface 103b3 of the projecting portion 103b2 of the lower surface 103b of the upper wall portion 103 in the housing upper member 10A and the side wall in the housing lower member 10B Of the inner surface 102c of the portion 102, a portion adjacent to the upper end 102b abuts. With these configurations, when the housing 10 is formed by engaging the housing upper member 10A and the housing lower member 10B, liquid leakage from the boundary between the side wall portion 102 and the upper wall portion 103 can be prevented. .

  Although the case 10 is formed of two members (the case upper member 10A and the case lower member 10B) in the illustrated example, the invention is not limited thereto, and the case 10 is formed of one member. It may be formed of three or more members.

  The internal space 11 is a space contained in the housing 10, and in the illustrated example, it is in communication with the outside through the holes 12 and 12, but the other part is a closed structure. In the illustrated embodiment, the bottom wall portion 101, the side wall portion 102, and the upper wall portion 103 surrounding the internal space 11 constitute a main body portion of the housing 10.

  The inner wall surface of the housing refers to the wall surface of the wall of the housing facing the internal space, and in the illustrated embodiment, the bottom wall 101, the side wall 102, and the upper wall that form the internal space 11 of the housing 10. It refers to the surface of the member such as 103 facing the internal space 11.

  The support portion 14 supports one or more (two in the illustrated example) containers 40, 40 containing the liquid L used for chromatography, and the containers 40, 40 from the first position to the second position described later. Holes 12 and 12 are formed as container guiding portions for guiding. The holes 12, 12 function as guide holes for guiding the containers 40, 40 respectively inserted and supported therein from a first position to a second position described later. The container guiding portion may have any structure as long as it can guide the container from the first position to the second position described later, and is not limited to the holes 12 and 12 formed in the support portion 14. The holes 12 and 12 are through holes that communicate the internal space 11 of the housing 10 with the outside. The holes 12 and 12 may be capable of inserting and supporting the containers 40 and 40 in the penetrating direction, and guiding the inserted containers 40 and 40 toward the blade 13, and the shape is specific It is not limited to the shape. In addition, "S" drawn on the outer surface 14a of the support part 14 shown to FIG. 1A is a label | marker of being the hole 12 which penetrates the container 40 which accommodated the sample liquid (sample) as the liquid L, "B" Is a mark indicating that the hole 12 is for inserting a container 40 containing a developing liquid (buffer) as the liquid L.

  The internal space 11 of the housing 10 is a place for developing the chromatography. A chromatography carrier 30 which is a solid phase is disposed in the internal space 11. When the liquid L is in contact with part of the chromatography carrier 30 in the internal space 11, the liquid L is developed in the chromatography carrier 30, and chromatography is performed.

  Examples of the liquid L include a sample solution containing a substance to be detected in a solvent such as water, a developing solution, and a liquid containing a labeling substance in a solvent such as water. The solvent is typically water. As a sample solution, a sample solution containing a nucleic acid amplification product can be exemplified. Examples of the developing solution include phosphate buffer, Tris buffer, Good buffer, SSC buffer and the like. The developing solution may further contain a surfactant. The liquid L may be a mixed liquid in which one or more kinds of a sample liquid, a developing liquid, and a liquid containing a labeling substance are mixed. Also, as the liquid L, a liquid containing a coloring reagent or a dye may be used. In the drawings and the following description, the liquid contained in the plurality of containers 40 and the liquid discharged to the internal space 11 are all represented as the liquid L, but the liquids L contained in the plurality of containers 40 are liquids of different compositions. May be there. Further, the container 40 for containing the liquid L other than the sample liquid may be inserted through the hole 12 in advance.

  The chromatography carrier 30 installed in the internal space 11 of the housing 10 may be any solid phase carrier, and may be made of resin, metal, polysaccharide, mineral or the like, and may be a membrane, film, non-woven fabric, plate , Gel and the like. In the illustrated example, the chromatography carrier 30 is an elongated membrane (strip) but is not limited to this example. Preferably, the chromatography carrier 30 has a porous structure. Specific examples of the chromatography carrier 30 include, for example, paper, nitrocellulose membrane, polyether sulfone membrane, nylon membrane, dried various gels (silica gel, agarose gel, dextran gel, gelatin gel), silicon, glass, resin, etc. Can be mentioned.

  In part of the chromatography carrier 30, a capture substance for capturing a detection target substance can be arranged. For example, when the substance to be detected contains a nucleic acid, a nucleic acid that hybridizes to the nucleic acid can be used as a capture substance, and when the substance to be detected contains an antigen or an antibody, immunology with the antigen or antibody A reactive antibody or antigen can be used as a capture substance.

  The substance to be detected is preferably one that can be visually detected on the chromatography carrier 30. For example, if a substance to be detected is labeled with a visually detectable labeling substance and developed on the chromatography carrier 30, visual detection is possible. Examples of labeling substances that can be detected visually include colored particles, dyes, and fluorescent substances. Examples of “colored particles” include metal (eg, gold, silver, copper, platinum etc.) particles, metal nanorods, metal nanoplates, latex particles containing dyes and fluorescent materials, silica nanoparticles including dyes and fluorescent materials, etc. Be The method of labeling the substance to be detected with a labeling substance capable of visual detection is not particularly limited. For example, when the substance to be detected contains a nucleic acid, the labeling substance to which the nucleic acid hybridizing to the nucleic acid is linked can be labeled with the labeling substance by bringing the substance to be detected into contact with the substance to be detected. For visual detection, at least a part of the wall portion of the case 10 surrounding the internal space 11 is formed of a material that transmits visible light so that the chromatography carrier 30 can be confirmed from the outside of the case 10 Is preferred. In particular, it is preferable that at least a portion 103c of the upper wall portion 103 of the housing 10 facing the portion where the chromatography carrier 30 is disposed be made of a material that transmits visible light.

  The structure of the chromatography carrier 30 used in the present embodiment is shown in FIG. In this example, the chromatography carrier 30 comprises a liquid receiver 31 and a detector 32. The liquid receiving portion 31 is disposed on one end side of the detecting portion 32, and the liquid L is supplied to this portion. The detection unit 32 is a portion in which a substance to be detected is developed. An absorption pad 33 is disposed on the other end side of the detection unit 32. Between the liquid receiving unit 31 and the detection unit 32, a labeling substance holding unit 34 for holding a labeling substance is disposed. When the detection target substance is contained in the liquid L supplied to the liquid receiving unit 31, the detection target substance is labeled by the labeling substance when passing through the labeling substance holding unit 34, and then moves to the detection unit 32. However, if the substance to be detected is labeled in advance, or if the liquid L contains a labeled substance, the labeled substance holding portion 34 is not necessary, and the liquid receiving portion 31 and the detecting portion 32 are adjacent to each other or The liquid receiver 31 can be omitted. The detection section 32 is formed with a capture area 321 in which the above-mentioned capture substance is disposed for capturing a detection target substance. The liquid receiving unit 31, the labeling substance holding unit 34, the detection unit 32, and the absorption pad 33 can be made of the above-described material as a material that can be used as the chromatography carrier 30. These may be comprised from the same member, and may be comprised from a different member. The liquid receiving unit 31, the labeled substance holding unit 34, the detection unit 32, and the absorption pad 33 can be disposed on the substrate 35 as illustrated. The substrate 35 can be made of, for example, a resin, metal, polysaccharide, mineral or the like. The substrate 35 can be partially or completely omitted. For example, by partially or completely omitting the base material corresponding to the lower portion of the liquid receiving portion 31, the flexibility of the liquid receiving portion 31 is improved, and the resistance when pushing the container 40 toward the blade is reduced. Can. Similar effects can be obtained by using a highly flexible substrate. The chromatography carrier 30 is not limited to one having the structure shown in FIG. 9, and one having a suitable structure can be used according to the target chromatography.

  As another example of the chromatography carrier 30, as shown in FIG. 10, a chromatography carrier 30 comprising only the detection unit 32 without the liquid receiving unit 31, the labeled substance holding unit 34, the absorption pad 33, and the base material 35. Can be mentioned. Further, although not shown, it is also possible to use a chromatography carrier 30 consisting only of the detection unit 32 and the base material 35 supporting the same.

  In the present embodiment, when the chromatography carrier 30 includes the labeling substance holding unit 34 in the internal space 11 of the housing 10, the liquid receiving unit 31 contacts the liquid L leaked from the container 40, but detection The portion 32 and the absorbent pad 33 are disposed so as not to be in direct contact with the leaked liquid L. As described above, when the liquid L contains a labeling substance, the labeling substance holding portion 34 can be omitted from the chromatography carrier 30. Further, as described above, it is also possible to use the chromatography carrier 30 consisting only of the detection unit 32, and the chromatography carrier 30 consisting only of the detection unit 32 and the base material 35. When using the chromatography carrier 30 in which the labeling substance holding portion 34 is omitted, the chromatography carrier 30 consisting only of the detection portion 32 shown in FIG. 10, or the chromatography carrier 30 consisting only of the detection portion 32 and the substrate 35 The liquid L leaked from the container 40 may be disposed to be in direct contact with the detection unit 32.

  In the following description, the portion of the chromatography carrier 30 in direct contact with the liquid L leaked from the container 40 is referred to as a liquid contact portion 30L.

  In the internal space 11 of the housing 10, there is formed a carrier installation portion 16 which is erected from the bottom wall portion 101 into the internal space 11 and on which the chromatography carrier 30 is installed. The carrier setting unit 16 is between the bottom wall portion 101 and the upper wall portion 103 in the opposing direction of the bottom wall portion 101 and the upper wall portion 103 of the housing 10, and the upper wall portion 103 of the chromatography carrier 30. It is configured such that it can be installed so as to face the portion 103 c of the upper wall portion 103 at a closer position. The detection unit 32 of the chromatography carrier 30 and the absorption pad 33 are disposed on the upper surface 16 a of the carrier placement unit 16. On the other hand, the liquid contact portion 30 </ b> L is disposed between the support portion 14 of the housing 10 and the blade 13. The detection unit 32 and the absorption pad 33 of the chromatography carrier 30 may be fixed to the upper surface 16 a of the carrier installation unit 16 by an adhesive or an adhesive tape. The upper surface 16 a of the carrier setting unit 16 is at a position where the liquid L leaking from the container 40 cut by the blade 13 does not reach, and specifically, when the housing 10 is installed on a horizontal surface, , At a position higher than the position where the liquid L is leaked. The upper surface 16a of the carrier placement unit 16 has a plan view shape corresponding to the portion including the detection unit 32 of the chromatography carrier 30 and the absorption pad 33, and in the illustrated example, has a shape having a longitudinal direction and a lateral direction. The carrier placement unit 16 is provided with a plurality of positioning protrusions at positions surrounding the top surface 16 a that restrict and position the movement of the chromatography carrier 30 in the direction along the top surface 16 a. The positioning projection includes a first positioning projection 16b disposed on both sides in the lateral direction of the upper surface 16a, and a second positioning projection 16c disposed on one longitudinal end of the upper surface 16a.

  Although the number of the holes 12 which are container guiding portions formed in the support portion 14 of the housing 10 is two in the illustrated example, it may be one or three or more, and it is used for the target chromatography It can be set according to the number of containers 40 containing the liquid L. For example, when using a sample solution and a developing solution as the liquid L, two holes 12 are formed. In this case, although not particularly limited, two holes 12, 12 are formed along the deployment direction at a position opposite to the position where the chromatography carrier 30 is disposed in the internal space 11, and the holes on the downstream side in the deployment direction 12, inserting the container 40 containing the sample liquid and inserting the container 40 containing the developing liquid into the hole 12 on the upstream side in the developing direction results in that the amount of the detection target substance reaching the detection unit 32 increases. preferable. Further, when the number of containers 40 is two or more, each container 40 may have a different shape, color tone, or pattern in order to easily distinguish the respective containers, and the liquid L contained in each container 40 is It may be colored to have different color tones. If two or more containers 40 having different shapes are used, each hole 12 may also have different shapes to fit each container shape.

  The lid 20 is attached to the housing 10 and configured to cover the support 14 of the housing 10. The lid 20 supports the support 14 so that when the liquid is present between the support 14 and the lid 20, the liquid does not leak to the outside of the inspection device 1 under normal use conditions. cover.

  In the present embodiment, the lid 20 includes the lid main portion 21 and the lid peripheral wall portion 22 starting from the outer peripheral edge of the lid main portion 21 and extending toward the inner surface 21 a of the lid main portion 21.

  At least one of the housing 10 and the lid 20 includes a lid guiding unit 50 for guiding the lid 20 to the housing 10.

  In the present embodiment, in the case 10, the support portion 14 is formed at a position projecting outward from the upper wall portion 103, and the support portion 14 and the upper wall portion 103 enclose the support portion 14 and a lid 20 are connected by a lid attachment unit 15 to be attached.

  In the present embodiment, the lid guiding portion 50 is formed on the housing side screwing portion 51 formed on the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall 22 of the lid 20. It is comprised from the formed housing side screwing part 51 and the lid side screwing part 52 which can be screwed. The lid 20 mounted on the housing 10 is rotated by the housing side screwing portion 51 and the lid side screwing portion 52 about the direction X in which the support portion 14 of the housing 10 and the lid 20 face each other as an axis. When moved, the lid 20 is guided by the lid guide 50 and moves in the direction X toward the support 14 of the housing 10.

  In order to make it easy to turn the lid 20, a plurality of recessed grooves 23a extending along the direction X are circumferentially spaced on the outer surface 22b of the lid peripheral wall 22 of the lid 20 as illustrated. It is preferable to form the non-slip portion 23 formed by vacancy. In addition, on the outer surface 21b of the lid main portion 21 of the lid 20, it is possible to form a marker 21c that allows visual identification of the rotational direction.

  The lid guiding portion 50 is not limited to the screwing portion illustrated, but may be in another aspect. For example, the screw attachment portion is not formed on the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall portion 22 of the lid 20. The aspect which the lid 20 slides and is guided with respect to the housing | casing 10 can be illustrated as another aspect, while the outer peripheral surface 15a of this and the internal peripheral surface 22a of the lid peripheral wall part 22 of the lid 20 contact | abut. Further, no screwing portion is formed on the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall portion 22 of the lid 20, and the lid mounting portion 15 of the housing 10 Another aspect in which the cover 20 is guided relative to the housing 10 while the outer peripheral surface 15a of the cover 20 is in contact with the inner peripheral surface 22a of the cover peripheral wall 22 of the cover 20 via one or more seal members. It can be illustrated as The sealing material can be formed of an elastically deformable material such as rubber. Each sealing material may be fixed to one of the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall 22 of the lid 20.

  The blade 13 cuts the containers 40, 40 supported by the support 14 and leaks the liquid L from the containers 40, 40 to a position where it contacts the liquid contact portion 30 L of the chromatography carrier 30. In the present embodiment, as the blade 13, a plate-like blade having a blade edge 131 formed on part of the sides and a base end 134 formed on the other side as shown in FIG. 2 is used. In the vicinity of the blade edge portion 131, the two side surfaces 132 and 133 are both blades which intersect at the blade edge portion 131 while being inclined together, but this is not limited thereto. Only one of the two side surfaces 132 and 133 is inclined It may be a single-edged blade intersecting at the cutting edge portion 131 while being flat. In the illustrated embodiment, the blade 13 is a rectangular plate-like blade, but plate-like blades of other shapes such as parallelograms, trapezoids, triangles, circles and the like can also be suitably used. In the present embodiment, the plurality of containers 40, 40 are cut by one blade 13. However, the present invention is not limited to this, and the blade 13 corresponding to each container may be provided. The material which comprises the blade 13 should just be a material which can give the intensity | strength which can incise the containers 40 and 40, for example, metals, such as steel, stainless steel, and aluminum, ceramics, and resin.

  The blade 13 is fixed to the inner wall surface of the housing 10 so as to be disposed in the inner space 11 of the housing 10. In the present embodiment, the blade 13 is disposed and fixed to the inner wall surface 101 a of the bottom wall portion 101 which is a wall portion facing the holes 12 and 12 in the internal space 11 of the housing 10. It is not limited. On the inner wall surface 101 a of the bottom wall portion 101, a fixing portion 17 for fixing the blade 13 is formed. In the present embodiment, the fixing portion 17 fixes the proximal end portion 134 of the blade 13 so as to sandwich the blade 13 from both sides in the thickness direction, and the cutting edge portion 131 of the blade 13 supports the support portion 14 in the internal space 11. It is arranged to face. In the present embodiment, the fixed portion 17 is arranged on the bottom wall portion 101 so as to be aligned with the carrier mounting portion 16. When the inspection device 1 of the present embodiment is placed on a horizontal surface, the upper surface 17 a of the fixing portion 17 is located lower than the upper surface 16 a of the carrier installation portion 16. The carrier setting portion 16 and the fixing portion 17 are connected by the connection portion 18 provided upright in the internal space 11 from the bottom wall portion 101, and the upper surface 16 a of the carrier setting portion 16 and the upper surface 17 a of the fixing portion 17 Are connected by the upper surface 18 a of the connecting portion 18 inclined from the upper surface 16 a of the carrier mounting portion 16 to the upper surface 17 a of the fixed portion 17. Third positioning projections 17 d are further formed on both sides of the top surface 17 a of the fixing portion 17 for fixing the blade 13 in the lateral direction. As the containers 40, 40 are guided from the first position described later to the second position and moved, the liquid of the chromatography carrier 30 disposed between the blade 13 fixed to the fixing portion 17 and the holes 12, 12 When the contact portion 30L moves toward the fixed portion 17, the movement of the liquid contact portion 30L is restricted in the direction in which the containers 40, 40 are guided.

  As the container 40, a container that can receive the liquid L used for chromatography, can be inserted into and supported by the holes 12 and 12 of the housing 10, and can be cut by the blade 13 can be used. The container 40 is typically a resin container. As the container 40, a tubular container extending in one direction and having one end closed and an open / close portion at the other end is preferable. Specifically, as illustrated, when supported by the support 14, the outer side of the housing 10 is provided. It is possible to use a container having an opening and closing portion 41a at an outer end 41 which is an end of the container 10 and an inner end 42 which is an inner end of the housing 10 is closed. The container 40 provided with the opening and closing part 41a at the outer end 41 as shown in the drawing is a hinge part 401 and a knob part 402 which are projected outward at the periphery 400 of the opening and closing part 41a. And, as such a container 40, a microtube used for a nucleic acid amplification reaction or a biochemical test can be exemplified.

  The container 40 to be inserted and supported is urged toward the axial center along the penetrating direction of each hole 12 at a position surrounding each of the holes 12 of the support portion 14, and the inside of each hole 12 of the container 40 is An elastic member 190 is disposed to maintain the posture of the seat. Specifically, the elastic member 190 is a pair of the inner surface 14b on the side of the inner space 11 of the support portion 14, which protrudes toward the inner space 11 from a pair of opposing positions around the holes 12 The elastic support pieces 191 and 192 of FIG. The pair of elastic support pieces 191 and 192 is disposed such that the surface 191 a of one elastic support piece 191 and the surface 192 a of the other elastic support piece 192 face each other. The surface 191 a of one elastic support piece 191 and the surface 192 a of the other elastic support piece 192 are formed to be closer as the distance along the direction X from the inner surface 14 b of the support portion 14 increases. The pair of elastic support pieces 191 and 192 sandwiches the container 40 inserted in each hole 12 from the side, and biases the container 40 toward the axial center along the penetrating direction of each hole 12 to maintain the posture. The pair of elastic support pieces 191 and 192 are elastically deformable and are pushed open following the shape of the container 40 as the container 40 is pushed toward the blade 13, so that the outer end from the inner end 42 as illustrated. Even in the case of using the container 40 having a shape in which the width increases toward the end 41, the posture of the container 40 can be stabilized. In addition, if the elastic support pieces 191 and 192 are in the elastically deformable range, the containers 40 having different widths can be supported and stabilized. In the present embodiment, the position of the container 40 relative to the blade 13 can be stabilized by holding the posture of the container 40 inserted in each hole 12 by the elastic member 190 including the pair of elastic support pieces 191 and 192. The amount of liquid L leaked from the container 40 can be stabilized.

  Subsequently, with reference to FIGS. 3A to 4C, a mechanism for cutting the containers 40 and 40 inserted and supported in the holes 12 and 12 of the support portion 14 with the blade 13 will be described.

  FIGS. 3A and 4A show a state in which the containers 40 and 40 containing the liquid L are inserted into and supported by the holes 12 and 12 of the support portion 14. At this time, the inner ends 42 and 42 of the containers 40 and 40 face the cutting edge portion 131 of the blade 13. As described above, the container is supported by the support portion, and the position of the container in a state where one end of the container is opposed to the blade edge of the blade is referred to as a “first position”.

  The containers 40, 40 supported in the holes 12, 12 of the support portion 14 and positioned at the first position are pushed toward the blade 13, and the inner ends 42, 42 of the containers 40, 40 are fixed to the fixing portion 17 of the housing 10. 3B and 4B (the state before the liquid L leaks) and FIGS. 3C and 4C (the state after the liquid L leaks) in which the state of complete movement until contact is shown. At this time, the containers 40, 40 are cut by the blade 13 from the side of the inner ends 42, 42 of the containers 40, 40. As described above, the container is supported by the support portion, guided by the container guide portion, and the position of the container in the state of being moved until it is cut by the blade is set to the “second position”. As shown in FIGS. 3B, 3C, 4B and 4C, in the present embodiment, the container in the second position is in contact with a part of the housing other than the blade.

  When the containers 40, 40 supported by the holes 12, 12 of the support portion 14 are guided from the first position to the second position, the containers 40, 40 are cut by the blade 13 and cut into the containers 40, 40. , 43 are formed, and the liquid L stored in the containers 40, 40 leaks to the position contacting the liquid contact portion 30L of the chromatography carrier 30 through the notches 43, 43.

  Further, in the present embodiment, the liquid contact portion 30L of the chromatography carrier 30 is accommodated in the housing 10 so as to be positioned between the support portion 14 and the blade edge portion 131 of the blade 13, whereby the containers 40, 40 are obtained. And the chromatography carrier 30 are cut by the blade 13. When the containers 40, 40 are in the first position, the inner end 42 of the containers 40, 40 and the cutting edge 131 of the blade 13 are opposed to each other with the liquid contact portion 30 L of the chromatography carrier 30 interposed therebetween. When the containers 40, 40 supported by the support portion 14 are guided and moved from the first position to the second position, the containers 40, 40 together with the liquid contact portion 30L of the chromatography carrier 30 are on the side of the inner ends 42, 42. The incisions 43 and 43 are formed by cutting with the blade 13. According to this embodiment, it is easy for the liquid L leaked from the containers 40 and 40 to contact and permeate the liquid contact portion 30L of the chromatography carrier 30. The inspection device 1 having this feature is preferable because the liquid L leaking from the containers 40, 40 can be efficiently brought into contact with the chromatography carrier 30.

  In the present embodiment, as shown in FIG. 3A and FIG. 4A, with the containers 40 and 40 supported by the support portion 14 of the housing 10 and positioned at the first position, the lid 20 is the housing 10. It mounts to the lid mounting part 15 which encloses the support part 14 of this. At this time, the housing side screwing portion 51 on the outer peripheral surface 15a of the lid mounting portion 15 of the housing 10 and the lid side screwing portion 52 on the inner peripheral surface 22a of the lid peripheral wall portion 22 of the lid 20 Screw it together. Thereby, the support portion 14 of the housing 10 and the outer portions 40 a and 40 a of the containers 40 and 40 are covered by the lid 20. Subsequently, when the lid 20 is rotated about the direction X in which the support 14 of the housing 10 and the lid 20 face each other, the lid 20 is guided by the lid guiding portion 50, It moves toward the support 14 of the housing 10 along X. In the process of this movement, the main lid portion 21 of the lid 20 abuts on the outer ends 41 of the containers 40, 40, whereby the containers 40, 40 are pushed toward the blade 13, as shown in FIGS. 3B, 3C. , To the second position shown in FIGS. 4B and 4C.

  The inspection device of the present invention is not necessarily provided with a lid as in this embodiment. For example, the inspection device does not include the lid, and the user presses the container supported by the support portion of the housing from the outside of the housing using an external force by operating the fingertip, the device, and the like. The container may be configured to move from the first position to the second position.

  In the inspection device 1 of the present embodiment, at least a part of the outer surface 102 a of the side wall portion 102 corresponding to the side surface of the housing 10 is formed with the wavelike surfaces 102 d and 102 d. The wavy surfaces 102d and 102d are surfaces in which a plurality of grooves 102e extending in a direction in which the bottom wall portion 101 and the upper wall portion 103 of the housing 10 face each other are continuously formed. By forming the wavelike surfaces 102 d and 102 d on the outer surface 102 a of the side wall portion 102, slippage can be prevented when the user handles the inspection device 1 with his / her hand. In addition, when preparing a plurality of inspection devices 1 of the present embodiment and arranging so that the outer surface 102 a of the side wall portion 102 of the housing 10 is in contact with each other, the waved surfaces 102 d can be engaged with each other. The wavy surface is an example of the uneven surface, and in the inspection device 1 of this embodiment, the wavy surface 102d may be replaced with an uneven surface having another shape. The uneven surface is a surface in which a plurality of grooves extending in one direction are continuously formed, and a flat surface may be formed between adjacent grooves. The shape of the cross section by a plane perpendicular to the extending direction of each of the plurality of grooves in the uneven surface is not particularly limited. Although the case where the shape of the cross section is a curved line is a wavelike surface as illustrated, the shape of the cross section may be a rectangle, a U-shape, or the like.

<First Feature of Inspection Device of First Embodiment>
The inspection device 1 of the first embodiment is
The length of the containers 40, 40 in the state of being supported by the support portion 14 along the direction guided by the holes 12, 12 (referred to as the “guiding direction Y”) is M,
When the containers 40, 40 are supported by the support portion 14 and guided from the first position to the second position, the maximum length along the guiding direction Y of the notches 43, 43 formed in the containers 40, 40 is taken as D When you
A first feature is that D / M is 0.2 or more.

The effects of the first feature will now be described with reference to FIG.
FIG. 7 (3) is a schematic cross-sectional view for explaining the difficulty of leakage of the liquid L when the incision 43 is formed only from the inner end 42 of the container 40 in the portion surrounding the liquid phase L. It is a figure, FIG. 7 (4) is the side view which looked at the container 40 shown in FIG. 7 (3) from the left of the figure. 7 (3) and (4), let the container 40 in which the notch | incision 43 is formed only in the part which encloses the liquid phase L among the containers 40 is made into "the comparative example 1" for convenience. In the container 40 of Comparative Example 1, the liquid L is accommodated in the container 40 having a length M along the guiding direction Y from the inner end 42 to a height position of less than 0.2 M, and the gas phase G is accommodated in the remaining portion. From the side of the inner end 42 of the container 40, a cut 43 of maximum length D along the guiding direction Y is formed. In Comparative Example 1, the container 40 is vertically supported from the inner end 42 of the container 40 to the liquid surface of the liquid L in a state where the container 40 is supported such that the guiding direction Y is the vertical direction and the inner end 42 is the vertical direction. The distance along the direction is greater than the maximum length D of the incision 43. In Comparative Example 1, the cut 43 is present only in the portion surrounding the liquid phase L in the container 40 in a state in which the container 40 is supported such that the guiding direction Y is in the vertical direction and the inner end 42 is in the vertical direction. Do. In Comparative Example 1, when the liquid L in the container 40 leaks out of the container through the incision 43, the gas phase G tends to have a negative pressure at the start of leakage and it is difficult for the liquid L to leak out of the container 40 through the incision 43 It is. In addition, when the container 40 is a microtube, it is general that there is no vent other than the cut | notch 43. As shown in FIG.

  On the other hand, in FIG. 7A, using the inspection device 1 of the first embodiment provided with the first feature, the container 40 is supported by the support portion 14 of the housing 10, and from the first position to the second position FIG. 7B is a cross-sectional schematic view of the container 40 in which the incision 43 is formed from the side of the inner end 42 shown in FIGS. 3B and 4B, which occurs when the container 40 is moved, and FIG. ) Is a side view of the container 40 seen from the left side of the drawing. The container 40 shown in FIGS. 7 (1) and 7 (2), in which the cut 43 is formed, is referred to as “Example 1” for convenience. In the container 40 of Example 1, the liquid L is accommodated in the container 40 having a length M along the guiding direction Y from the inner end 42 to a height position less than D, and the vapor phase G is accommodated in the remaining portion. From the side of the inner end 42 of the container 40, a cut of maximum length D along the guiding direction Y is formed. At this time, since D is 0.2 M or more, if the container 40 is supported so that the guiding direction Y is vertical and the inner end 42 is vertically downward, the two upper end portions 43A and 43A of the notches 43 are Both are located in the part which encloses the gaseous phase G of the container 40, and connect the gaseous phase G in the container 40, and external air. Then, when the liquid L in the container 40 leaks out of the container through the incision 43, air is supplied to the vapor phase G from the outside of the container through the upper end portions 43A and 43A of the incision 43 at the time of leakage start. Is unlikely to be a negative pressure, and the liquid L can smoothly leak from the container 40 through the incision 43. In order to obtain this effect, the liquid L can be stored at a depth of less than D, ie, at least less than 0.2 M.

  In the inspection device 1 of the first embodiment, the cutting edge portion 131 of the blade 13 extends in a straight line and is disposed to be orthogonal to the guiding direction Y. Although it has a U-shaped symmetrical shape as in Example 1, it is not limited to this aspect. As an example, in FIG. 7 (5), the inspection of the same structure as that of the first embodiment except that the cutting edge portion 131 of the blade 13 extends on a straight line and is disposed inclined with respect to the guiding direction Y. Cutting 43 is formed from the side of the inner end 42 which is generated when the container 40 is supported by the support portion 14 of the housing 10 and the container 40 is moved from the first position to the second position It is a cross-sectional schematic diagram of the container 40, and FIG. 7 (6) is the side view which looked at the container 40 shown in FIG. 7 (5) from the left of the figure. The container 40 shown in FIGS. 7 (5) and 7 (6), in which the cut 43 is formed, is referred to as “Example 2” for convenience. In the container 40 of Example 2, the liquid L is accommodated in the container 40 having a length M along the guiding direction Y from the inner end 42 to a height position less than D, and the vapor phase G is accommodated in the remaining portion. From the side of the inner end 42 of the container 40, a cut of maximum length D along the guiding direction Y is formed. At this time, D is 0.2 M or more. In the second embodiment, the notch 43 does not have a symmetrical shape, and one upper end of the notch 43 is supported when the container 40 is supported such that the guiding direction Y is the vertical direction and the inner end 42 is the downward direction. 43B is positioned vertically above the other upper end 43C. The maximum length D is the length of the incision 43 along the guiding direction Y from the inner end 42 to the upper end 43B. If the container 40 of Example 2 is supported so that the guiding direction Y is vertical and the inner end 42 is vertical, one upper end 43 B of the notch 43 encloses the gas phase G of the container 40. It is located in a part and connects the gas phase G in the container 40 and the outside air. When the liquid L in the container 40 leaks out of the container through the incision 43, air is supplied from the outside of the container to the gas phase G through the upper end 43B of the incision 43 when leakage starts, so the gas phase G is negative. It is difficult for pressure to be applied, and the liquid L can be smoothly leaked from the container 40 through the incision 43.

  That is, the containers 40 and 40 containing the liquid L to a depth of less than D on the side of one end and using the remaining portion as the gas phase G have the first feature that D / M is 0.2 or more The containers 40, 40 are supported on the support portion 14 of the inspection device 1 of the first embodiment in such a manner that the one end is directed downward in the vertical direction and the guiding direction Y substantially coincides with the vertical direction. When guided from the first position to the second position, notches 43, 43 are formed in the containers 40, 40 by the blade 13 to a portion surrounding the gas phase G. At this time, air is supplied from the outside of the container to the gas phase G through the portions connecting the gas phase G and the outside of the container from the notches 43 formed in the containers 40 and 40. , 40 when the liquid L leaks, at the beginning of the leak, the gas phase G does not easily become negative pressure, and the liquid L can leak smoothly.

  In the first feature, D / M may be 0.2 or more, more preferably 0.3 or more, and more preferably 0.4 or more. The upper limit of D / M is not particularly limited, but is usually 0.9 or less.

<Second Feature of Inspection Device of First Embodiment>
The inspection device 1 of the first embodiment is
The case 40 is positioned so that the liquid L is positioned on the side of the inner end 42 which is the end of the containers 40, 40 opposite to the cutting edge 131 of the blade 13 of the containers 40, 40 in the first position. From the inner end 42 of the container 40, 40 to the portion 40L1, 40L1 of the container 40, 40 where the liquid level L1 is located when the liquid L is contained in the container 40, 40 Of the notches 43, 43 formed in the containers 40, 40 when the containers 40, 40 are subsequently guided from the first position to the second position, with the distance along the vertical direction V being A. Let B be the maximum length along
A second feature is that B is greater than A.
That is, when the distance A is supported by the support unit such that the end of the container is directed downward in the vertical direction and is at the first position, the liquid is contained in the container from the end of the container It can be the distance along the vertical direction to the part where the liquid level is located. Here, "the end of the container is directed vertically downward" means that the end is positioned lower than the other end so that the liquid in the container can be positioned on the side of the end of the container. In addition to supporting the container so that the longitudinal direction of the container substantially coincides with the vertical direction as in Examples 3 and 4 described later, the support of the support portion is not only supported, but also Example 5 described later. And supporting the container such that the longitudinal direction of the container is inclined relative to the vertical direction. The maximum length B of the incision can be the maximum length along the vertical direction of the incision formed in the container when the container is guided from the first position to the second position.

Here, the effects of the second feature will be described with reference to FIGS. 8-1 and 8-2.
8-1 (3) is a container containing a liquid L and a gas phase G, using an inspection device having the same features as the inspection device 1 of the first embodiment except that B is A or less. 40 is not supported by the support portion 14 of the housing 10, and the cut 43 of the maximum length B (A or less) in the vertical direction V that occurs when the container 40 is moved from the first position to the second position It is a cross-sectional schematic diagram of formed container 40, and Drawing 8-1 (4) is a side view which looked at container 40 shown in Drawing 8-1 (3) from the left of a figure. The container 40 in which the cut 43 having the maximum length B in the vertical direction V of A or less shown in FIGS. 8-1 (3) and (4) is formed is referred to as “comparative example 2” for convenience. When the container 40 of Comparative Example 2 is in the second position in the device for inspection, the cut 43 is present only in the portion surrounding the liquid phase L in the container 40, the liquid L in the container 40 passes through the cut 43. When leaking out of the container, the gas phase G tends to be negative pressure at the start of leakage, and it is difficult for the liquid L to leak out of the container 40 through the incision 43.

  On the other hand, in FIG. 8-1 (1), the container 40 is supported by the support portion 14 of the housing 10 using the inspection device 1 of the first embodiment provided with the second feature, and from the first position to the second position. Fig. 8-1 (2) is a schematic cross-sectional view of the container 40 in which the notches 43 having the maximum length B (larger than A) in the vertical direction V, which are produced when the container 40 is moved to the position, are formed. Fig. 8-1 is a side view of the container 40 shown in Fig. 8-1 (1) as viewed from the left of the figure. A container 40 in which a cut 43 having a maximum length B in the vertical direction V greater than A in FIGS. 8-1 (1) and (2) is referred to as “Example 3” for convenience. When the container 40 of Example 3 is at the second position in the inspection device 1 of the first embodiment, the two upper end portions 43A and 43A of the notches 43 are both in the portion of the container 40 surrounding the gas phase G. It locates and connects the gaseous phase G in container 40, and the open air. Then, when the liquid L in the container 40 leaks out of the container through the incision 43, air is supplied to the vapor phase G from the outside of the container through the upper end portions 43A and 43A of the incision 43 at the time of leakage start. Is unlikely to be a negative pressure, and the liquid L can smoothly leak from the container 40 through the incision 43.

  8-1 (5), for inspection of the same structure as that of the first embodiment except that the cutting edge portion 131 of the blade 13 extends on a straight line and is disposed to be inclined with respect to the guiding direction Y. The maximum length B (larger than A in the vertical direction V) generated when the container 40 is supported by the support portion 14 of the housing 10 using the device 1 and the container 40 is moved from the first position to the second position 8-1 (6) is the side view which looked at the container 40 shown to FIGS. 8-1 (5) from the left of the figure. The container 40 in which the cut 43 having the maximum length B in the vertical direction V larger than A shown in FIGS. 8-1 (5) and (6) is referred to as “Example 4” for convenience. When the container 40 of the fourth embodiment is at the second position in the inspection device 1 of the first embodiment, one upper end 43B of the notch 43 is vertically above the other upper end 43C, and One upper end 43 </ b> B of the notch 43 is located in a portion of the container 40 surrounding the gas phase G, and connects the gas phase G in the container 40 and the outside air. Then, when the liquid L in the container 40 leaks out of the container through the incision 43, air is supplied from the outside of the container to the vapor phase G through the upper end 43B of the incision 43, so that the vapor phase G hardly has negative pressure. The liquid L can smoothly leak from the container 40 through the incision 43.

  In the third embodiment shown in FIG. 8-1 (1) and the fourth embodiment shown in FIG. 8-1 (5), when the container 40 is supported by the support portion 14 of the housing 10, the longitudinal direction of the container 40 is This is an example substantially corresponding to the vertical direction. However, in the inspection device 1 having the second feature of the first embodiment of the present invention, the longitudinal direction of the container 40 when supported by the support portion 14 needs to be substantially coincident with the vertical direction It may be inclined relative to the vertical direction. For example, in FIG. 8-2 (7), the container 40 is supported by the support portion 14 of the housing 10 so that the longitudinal direction of the container 40 is inclined with respect to the vertical direction, and the container is moved from the first position to the second position 40 of the invention similar in construction to the embodiment illustrated in FIGS. 1A-4C except that the blade 13 can be guided and the blade 13 is arranged to form a cut 43 in the shape illustrated in the container 40. Using the inspection device 1 having the second feature of the first embodiment, the container 40 is supported by the support portion 14 of the housing 10 and the container 40 is moved from the first position to the second position It is a cross-sectional schematic diagram of the container 40 in which the cut | notch 43 of the largest length B (larger than A) in the perpendicular direction V which arises is formed. The maximum length B of the incision 43 in the vertical direction V shown in FIG. 8-2 (7) is along the vertical direction V from the inner end 42 of the container 40 to the portion 40L1 of the container 40 where the liquid level L1 is located. The container 40 in which the incision 43 larger than the distance A is formed is referred to as “fifth embodiment” for convenience. When the container 40 of Example 5 is at the second position in the inspection device 1 of the present embodiment, the upper end 43D of the notch 43 located vertically above the portion of the container 40 surrounding the gas phase G It locates and connects the gaseous phase G in container 40, and the open air. When the liquid L in the container 40 leaks out of the container through the incision 43, air is supplied from the outside of the container to the gas phase G through the upper end 43D of the incision 43 when leakage starts, so the gas phase G is negative. It is difficult for pressure to be applied, and the liquid L can be smoothly leaked from the container 40 through the incision 43.

That is, the containers 40, 40 containing the liquid L and the gas phase G are placed on the support portion 14 of the inspection device 1 of the first embodiment having the second feature that B is larger than A. Allowing the liquid L to be positioned on the side of the inner end 42, 42, which is one end of the liquid, and to be in the first position;
When, after Step 1, Step 2 of guiding the containers 40, 40 from the first position to the second position is performed,
By the blade 13, notches 43, 43 are formed in the containers 40, 40 to a portion surrounding the gas phase G. At this time, air is supplied from the outside of the container to the gas phase G through the portions connecting the gas phase G and the outside of the container from the notches 43 formed in the containers 40 and 40. 40, when the liquid L leaks, the gas phase G hardly has negative pressure from the start of the leak, and the liquid L can smoothly leak.

<Other Embodiments of Fixed Portion>
As a suitable modified example of the device 1 for inspection of the first embodiment, an example in which the fixing portion 17 for fixing the blade 13 has a structure shown in FIG. 5A can be mentioned.

  The fixing portion 17 shown in FIG. 5A includes a tapered portion 17 b formed so that the dimension in the thickness direction of the blade 13 becomes smaller as it approaches the cutting edge portion 131 of the blade 13.

  Shown in FIG. 5B when the container 40 is supported by the support portion 14 and guided from the first position to the second position, using the modification of the inspection device 1 of the first embodiment provided with the fixed portion 17 provided with the tapered portion 17b. As described above, the tapered portion 17 b of the fixing portion 17 is inserted into the notch 43 formed in the container 40. As a result, the opening width of the notches 43 is expanded, and the leakage of the liquid L contained in the container 40 through the notches 43 is further smoothened.

  As another preferable modification of the inspection device 1 of the first embodiment, an example in which the fixing portion 17 for fixing the blade 13 has a structure shown in FIGS. 6A and 6B can be given.

  The fixing portion 17 shown in FIG. 6A includes a tapered portion 17 c formed so that the dimension in the thickness direction of the blade 13 becomes smaller as it approaches the cutting edge portion 131 of the blade 13. The cross section of the taper part 17c in the II line in FIG. 6A is shown in FIG. 6B. As shown in FIG. 6B, the tapered portion 17c has an uneven surface 17c3 as a side surface including a portion 17c1 protruding in the thickness direction of the blade 13 and a portion 17c2 recessed in the thickness direction of the blade 13.

  When the container 40 is supported on the support portion 14 and guided from the first position to the second position, using the modification of the inspection device 1 of the first embodiment provided with the fixing portion 17 including the tapered portion 17c, The tapered portion 17 c is inserted into the notch 43 formed in the container 40. As a result, the opening width of the notches 43 is expanded, and the leakage of the liquid L contained in the container 40 through the notches 43 is further smoothened. Furthermore, a gap is formed between the end face of the cut 43 formed in the container 40 and the uneven surface 17c3 of the tapered portion 17c, and the leakage of the liquid L through the cut 43 is further smoothened.

  The inspection device 1 of the above embodiment of the structure illustrated in FIGS. 1A to 4C was produced. As the chromatography carrier 30, the chromatography carrier 30 in which the liquid receiving unit 31, the labeling substance holding unit 34, the detection unit 32, and the absorption pad 33 are disposed on the substrate 35 having the structure illustrated in FIG. 9 was used.

  When the container 40 is supported by the support 14 of the housing 10 of the inspection device 1, the container 40 has an open / close portion 41 a at the outer end 41 which is the outer end of the housing 10. A microtube for biochemical testing was used, with the inner end 42 at the end closed. When the container 40 was supported by the support 14 of the inspection device 1, the length M along the guiding direction Y was 21.5 mm.

  The inspection device 1 is installed such that the guiding direction Y coincides with the vertical direction V, and is supported by the support portion 14 of the housing 10 so that the inner end 42 of the container 40 is directed downward in the vertical direction. The maximum length D (= B) along the guiding direction Y (= vertical direction V) of the incision 43 formed in the container 40 when guided to the position was 7.5 mm. D / M was 0.349.

  The inspection device 1 is installed so that the guiding direction Y coincides with the vertical direction V, the inner end 42 is vertical with the container 40 containing the development liquid L for chromatography in the range of 5 to 100 μL and containing the air in the remaining portion. The developing solution L was leaked and developed by supporting the supporting portion 14 of the housing 10 so as to face downward in the direction and guiding from the first position to the second position. It was determined that the development was successful when the developing solution L was developed on the absorption pad 33 of the chromatography carrier 30 after 10 minutes had elapsed since the container 40 was moved to the second position. Twenty tests were performed for one fluid volume, and the number of successful deployments was recorded. The distance A from the inner end 42 to the liquid surface when the container 40 containing the developing liquid L of each liquid amount is disposed so that the inner end 42 is directed downward in the vertical direction along the vertical direction The following table shows the results of measurement of the deployment success rate. Under the condition that the maximum length B (7.5 mm) of the notches 43 in the vertical direction is larger than the liquid level height A, it is confirmed that the deployment success rate is 100%.

1: Inspection device 10: Case 12: Hole (container guide portion)
13: blade 131: cutting edge portion 14: support portion 17: fixed portion 17b: tapered portion 17c3: uneven surface 30: chromatography carrier 40: container 42: inner end of container 40 (one end)
43: notches L: liquid Y: guiding direction V: vertical direction

Claims (5)

A testing device,
It has an enclosure for accommodating the chromatography carrier,
The housing is
A support for supporting a container containing a liquid used for chromatography;
And a blade for cutting the container supported by the support, and for leaking liquid from the container to a position contacting the carrier.
From the first position where one end of the container faces the cutting edge of the blade to the second position where the container is incised by the blade from the side of the end of the container with the support portion supporting the container , And a container guiding unit for guiding the container is formed;
Let M be the length of the container along the guiding direction, which is the direction of being guided by the container guiding portion in the state of being supported by the support portion,
Assuming that the maximum length of the cut formed in the container along the guiding direction is D when the container is supported by the support portion and guided from the first position to the second position,
A device for inspection characterized by D / M being 0.2 or more. A testing device,
It has an enclosure for accommodating the chromatography carrier,
The housing is
A support for supporting a container containing a liquid used for chromatography;
And a blade for cutting the container supported by the support, and for leaking liquid from the container to a position contacting the carrier.
From the first position where one end of the container faces the cutting edge of the blade to the second position where the container is incised by the blade from the side of the end of the container with the support portion supporting the container , And a container guiding unit for guiding the container is formed;
The distance along the vertical direction from the end of the container when the container is supported by the support portion to be at the first position to the portion where the liquid level is located when the liquid is contained in the container Assuming that A, and then guiding the container from the first position to the second position, when the maximum length along the vertical direction of the incision formed in the container is B,
A testing device characterized in that B is larger than A. The blade is a plate-like blade having a blade edge formed on a part of the sides and a base end formed on the other side,
A fixing portion is formed on the inner wall surface of the housing so as to clamp the base end of the blade from both sides in the thickness direction of the blade,
The fixing portion includes a tapered portion formed so that the dimension in the thickness direction of the blade becomes smaller as it approaches the edge of the blade,
The inspection device according to claim 1 or 2, wherein when the container is supported by the support portion and guided from the first position to the second position, the tapered portion of the fixing portion is inserted into the notch formed in the container.   The inspection device according to claim 3, wherein the tapered portion of the fixing portion has an uneven surface which protrudes and / or is recessed in the thickness direction of the blade. A method of performing chromatography using a test device, comprising:
The test device is
Chromatography carrier, and
A housing for containing the carrier;
The housing is
A support for supporting a container containing a liquid used for chromatography;
And a blade for cutting the container supported by the support, and for leaking liquid from the container to a position contacting the carrier.
From the first position where one end of the container faces the cutting edge of the blade to the second position where the container is incised by the blade from the side of the end of the container with the support portion supporting the container , And a container guiding unit for guiding the container is formed;
Supporting the container containing the liquid to be used for chromatography together with the gas phase on the support of the test device so as to be at the first position;
Guiding the container from the first position to the second position after the step 1;
Let A be the distance along the vertical direction from the end of the container supported by the support in step 1 to the liquid level in the container,
Assuming that the maximum length along the vertical direction of the incision formed in the container in step 2 is B,
A method characterized in that B is larger than A.

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