JP2019071974A - Retainer for laboratory animal - Google Patents

Abstract

To provide a retainer for a laboratory animal for making it possible to perform operation on the laboratory animal without applying excessive stress on the laboratory animal.SOLUTION: The retainer for a laboratory animal comprises: a body part including a penetration part and a gap part; a head housing part for housing the head of the laboratory animal by moving the inside of the penetration part in parallel with the body part's longer axis direction; a knob part for operating the head housing part and fixing it to the body part; and a barrier part comprising a slit. The barrier part is detachable from the body part or is attached to the body part as an opening/closing type; and, in a state in which the barrier part is attached to the body part, the slit is provided so that the body part's marginal part positioned on the base side is taken as a start point of the slit.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a holding device for holding experimental animals such as mice and rats. The invention also relates to a one way treatment of mice or rats.

  Animal experiments using experimental animals such as mice and rats have been conducted in order to obtain useful information to know the effects or effects of drug administration on humans. In animal experiments, operations such as drug administration and blood collection are often performed on the tail. In order to manipulate or treat the tail of the experimental animal, it is necessary to hold the experimental animal.

  A general laboratory animal holding device includes a cylindrical main body having a tail slit, a head storage for moving the head of the experimental animal in parallel with the long axis direction of the main body, and a head It consists of a knob part for operating a storage part and fixing to a main-body part (patent documents 1-3).

  These conventional types of laboratory animal holding devices require that when the laboratory animal is stored in the holding device, it be necessary to grasp the tail and pull the laboratory animal backwards, i.e., in the direction opposite to the laboratory animal's field of view. In other words, the experimental animal has to be handled such that the rear part is stored in the main part first, not from the head.

  When the experimental animal is treated backward, the experimental animal is overstressed, which is not preferable from the viewpoint of animal protection. In addition, it is possible that this stress changes the components in the body and influences the experimental results. Furthermore, the experiment operation can not be smoothly advanced by refusing the test animal from being stored in the main body using the back foot. In particular, when housed in a retainer, it is difficult to smoothly hold an animal if it is an inexperienced person, and there is also a concern that excessive stress may be given. Therefore, there has been a demand for a holding device capable of smoothly storing and removing experimental animals without stressing the experimental animals.

JP H8-47502 JP 2005-111108 A JP, 2013-94629, A

  The present invention solves the above-mentioned problems of the prior art, and it is an object of the present invention to smoothly carry out storage from a holding device and taking it out from the holding device without applying stress to experimental animals.

As a result of intensive studies on a holding device capable of smoothly storing and removing experimental animals without stressing the experimental animals, the present inventor has found that a main body having a penetration portion and a clearance portion, and a penetration portion A head storage unit for moving the inside in parallel with the long axis direction of the main unit, and a head storage unit for storing the head of the experimental animal, a knob unit for operating the head storage unit and fixing to the main unit, and a slit A barrier portion comprising: a barrier portion fixed to the main body portion, the barrier portion being removable from the main body portion or attached to the main body portion as an openable / closable member; It has been found that the above-mentioned problem can be solved by the experimental animal holding device in which the slit is provided with the slit starting from the side edge portion of the main body portion located on the base side.
The present invention is based on these findings.

Therefore, according to the present invention, (1) a main body having a penetration portion and a gap portion, and a head storage for moving the inside of the penetration portion parallel to the long axis direction of the main body and storing the head of the experimental animal A laboratory animal restraint apparatus comprising: a section, a knob section for operating the head storage section and fixing it to the main body section, and a barrier section having a slit, wherein the barrier section is the main body And the slit is located on the base side in a state that the slit is removable from the main body or attached to the main body as the opening / closing type, and the barrier is attached to the main body. The holding device, characterized in that the edge side is provided as a starting point,
(2) The holding device according to (1), wherein the barrier portion is a removable shutter that can be detached from the main body portion,
(3) The holding device according to (1) or (2), wherein the barrier portion further comprises a stopper.
(4) The holding device according to any one of (1) to (3), further including a base.
(5) The holding device according to (4), including a non-slip mechanism at the bottom of the base.
(6) The holding device according to any one of (1) to (5), which is for mice or rats.
(7) The holding device according to any one of (1) to (6), for use in one-way treatment of experimental animals.
(8) housing the mouse or rat from the head in a holding device, treating the tail of the mouse or rat, and removing the mouse or rat from the head from the holding device The method according to (8), wherein the one-pass treatment method and (9) the treatment is blood collection from tail vein or artery, drug administration, blood pressure measurement, or marking.

  In the holding device according to the present invention, the tail of the experimental animal such as blood collection and drug administration can be used without handling the experimental animal backward in any operation when storing the experimental animal in the holding device and removing the experimental animal from the holding device. It is possible to perform operations on Therefore, it is possible to manipulate the tail of the experimental animal without giving excessive stress to the experimental animal.

FIG. 1 is a perspective view of one embodiment of a laboratory animal retaining device of the present invention. It is a top view of one mode of a holding device for laboratory animals of the present invention. It is a left side view of one mode of a holding device for laboratory animals of the present invention. It is the front view and right side view of one mode of the barrier part used in the holding device for laboratory animals of the present invention. It is a figure which shows the state of the head storage part in the holding | maintenance apparatus for laboratory animals of this invention. It is a perspective view of the one aspect | mode of the main-body part used in the holding | maintenance apparatus for laboratory animals of this invention. It is a front view of the one aspect | mode of the main-body part used in the holding | maintenance apparatus for laboratory animals of this invention. It is a perspective view of another aspect of the holding | maintenance apparatus for laboratory animals of this invention. It is a top view of another aspect of the holding | maintenance apparatus for laboratory animals of this invention. It is a left view of another aspect of the holding | maintenance apparatus for laboratory animals of this invention.

  The experimental animal holding device of the present invention will be described below with reference to the drawings, which illustrate preferred embodiments and do not limit the scope of the technical concept of the present invention.

  FIGS. 1-3 is a figure which shows the one aspect | mode of the holding | maintenance apparatus for laboratory animals of this invention. 1 to 3 show a holding device for a mouse. The experimental animal holding device 1 moves the inside of the through portion 22 parallel to the long axis direction of the main portion 2 with the through portion 22 and the gap portion 21 and accommodates the head of the experimental animal. It comprises a head storage portion 6, a knob portion 4 for operating the head storage portion 6 and fixing it to the main body portion 2, and a barrier portion 5 provided with a slit. The laboratory animal holding apparatus 1 shown in FIG. 1 is provided with a removable shutter as the barrier section 5. The removable shutter can be easily removed from the main body by moving the shutter vertically or horizontally. Moreover, in the state in which the barrier part 5 was mounted | worn with the main-body part, the slit is provided by using the side edge part side of the main-body part 2 located in the base 3 side as a starting point.

  The shape of the main body 2 is not particularly limited as long as it can accommodate the experimental animal inside, and any shape can be used, but the influence on the body of the experimental animal is reduced as much as possible when it is accommodated. In order to prevent the generation of a gap and / or to enable easy insertion of the head storage portion 6 and enable smooth operation, it is preferable to have a cylindrical shape. The material of the main body portion 2 is not particularly limited, but it is preferable to use a transparent material such as an acrylic resin because the experimental animal inside can be confirmed.

  The main body portion 2 has a through portion 22 for housing the experimental animal therein (see FIG. 6). In the laboratory animal holding device 1 of the present invention, the laboratory animal can be stored from one end of the penetrating portion 22 and taken out from the other end.

  The main body 2 includes a gap 21 for sliding the knob 4 and the head storage 6. The width of the gap 21 is not particularly limited as long as the knob 4 and the head storage 6 can be slid. The body portion 2 can comprise a receiving portion 23 for inserting the barrier portion 5 (see FIG. 6).

  The laboratory animal holding apparatus 1 according to the present invention includes a head storage unit 6 for storing the head of a laboratory animal. The head storage portion 6 moves the inside of the through portion 22 of the main body portion 2 in parallel with the long axis direction of the main body portion 2 and fixes to the main body portion 2 at the point where the head storage portion 6 reaches the head of the experimental animal By placing the head of the experimental animal. The head storage portion 6 can be easily removed from the laboratory animal holding device 1 by sliding it to the end of the gap 21 of the main body 2. The material of the head storage portion 6 is not particularly limited as long as the head of the experimental animal can be stored, but it is preferable to use the same material as the main body portion 2. Also, a metal mesh or basket may be used.

  The head storage portion 6 preferably has a breathing hole 61 for the laboratory animal to breathe. The shape, size, and number of the breathing holes are not particularly limited as long as the experimental animal can breathe.

  The laboratory animal holding apparatus 1 of the present invention is provided with a knob 4 for operating the head storage 6 and fixing it to the main body 2. The head storage portion 6 and the knob portion 4 are connected by a bolt portion 41 as shown in FIG. Therefore, by sliding the knob portion 4, the head storage portion 6 can also be slid together in the through portion 22 of the main body portion 2.

  After the head of the experimental animal is stored in the head storage 6, the knob 4 and the head storage 6 are fixed to the main body 2. After fixation, manipulation or treatment is performed on the tail of the experimental animal. The manipulation or treatment for the tail includes, for example, injection insertion, specifically, blood collection from the tail vein or artery, and drug administration. Blood pressure measurement and individual identification (marking) can also be performed.

  The shape and material of the knob portion 4 are not particularly limited as long as the head storage portion 6 can be operated and fixed to the main body portion 2. Preferably, a screw type as shown in FIG. 1 is used. In the screw type head storage portion 6 as shown in FIG. 1, after sliding the head storage portion 6 to a predetermined position, the screw is rotated to fix the head storage portion 6 to the main body portion 2.

  The laboratory animal retaining device 1 of the present invention can be provided with a barrier 5 which is removable from the main body 2. In FIG. 1, a withdrawal shutter is illustrated as the barrier unit 5. As shown in FIG. 4, the barrier portion 5 is provided with a slit 52 for passing the tail portion of the experimental animal. Moreover, as shown in FIG. 4, the barrier portion 5 can be provided with a stopper 51.

  In the conventional laboratory animal holding device, when the experimental animal is stored in the holding device, the experimental animal is moved backward by pulling the tail in order to insert the tail into the tail slit installed in the main body. The In the laboratory animal holding apparatus of the present invention, the barrier portion is removable from the main body portion, or is attached to the main body portion as an opening / closing type, and the slit is attached to the base side in a state of being attached to the main body portion. It is provided as a starting point on the side edge of the main body portion located in (the portion of the main body portion surrounded by the dotted line in FIG. 7). By adopting the configuration of the present invention, the head of the experimental animal is first stored from one body end and the head of the experimental animal from the other body end without moving the experimental animal backwards. It became possible to move "one way" to take out the first. This “one-way” movement is a type of method in which the experimental animal is housed in the main body from the head and taken out from the head from the main body. The experimental animals have the tendency to move forward, and by adopting the “one-way” movement, smooth storage and removal can be performed without contradicting the habits of the experimental animals.

  If the barrier is removable from the main body, the shape of the barrier can be easily removed from the main body, has a slit for passing the tail of the experimental animal, and can hold the experimental animal It is not particularly limited. For example, as shown in FIG. 1, there is a shutter type barrier portion which is inserted into and removed from the receiving portion provided in the main body. Also included is an externally fitted barrier that covers the entire end of the body and secures it to the outer surface of the body. There is also an inner fitting type barrier portion which is fixed by being fitted into the through portion.

As an advantage of adopting a withdrawal shutter as the barrier part, the following can be mentioned.
1. When the experimental animal is stored, it does not open even when the experimental animal is violently pushed or when the knob portion is pushed too much, and it is possible to prevent the experimental animal from leaving.
2. It is only necessary to provide the receiving portion in the main body portion, and processing is easy.

  The barrier part included in the laboratory animal retaining device of the present invention may be openable / closable attached to the main body (not shown). In this case, the shape of the barrier portion is not particularly limited as long as it has a slit for passing the tail portion of the experimental animal and can hold the experimental animal. For example, there is a double door type that opens from the center to the left and right, and a hinge method that is attached to one end of the main body using a hinge. As means for fixing the barrier to the main body, means known to those skilled in the art such as a clip can be used.

  The material of the barrier portion is not particularly limited, and a known material such as a metal such as stainless steel or aluminum and a resin such as plastic can be used.

  A slit is provided in the barrier to pass the tail of the experimental animal. The slit is provided with the side edge portion of the main body portion located on the base side as the start point in the state where the barrier portion is mounted to the main body portion. The shape of the slit is not particularly limited as long as the tail portion of the experimental animal can be passed and the experimental animal can be retained, but a substantially elliptical shape or a substantially rectangular shape is preferable. The slit can also be extended to the opposite side edge portion starting from the side edge portion of the main body portion located on the base side, but it is preferable not to extend from the viewpoint of strength and easiness of processing. In addition, the width of the slit may be narrowed or increased in the middle. Only one slit may be provided, or two or more slits may be provided.

  The laboratory animal holding device 1 of the present invention can have a base 3. The laboratory animal holding apparatus 1 of the present invention can be made to stand by the base 3. The shape of the base 3 is not particularly limited as long as it is a shape that allows the laboratory animal holding device 1 of the present invention to stand on its own, and any shape can be used, but from the viewpoint of stability Preferred. The material of the base 3 is not particularly limited, but it is preferable to use the same material as the main body 2.

  The main body 2 can be fixed to the base 3. Examples of means for fixing the main body 2 to the base 3 include, but are not limited to, welding, brazing, screwing, bonding, press fitting, and the like.

  The base 3 preferably has a non-slip mechanism. By having the anti-slip mechanism, it is possible to prevent damage due to falling from an experimental desk or the like. The anti-slip mechanism is not particularly limited as long as the anti-slip device for laboratory animals of the present invention can prevent slipping, but it is preferable to install an anti-slip mat or the like at the bottom of the base 3.

  FIG. 4 shows an insertion and withdrawal type shutter which is one aspect of the barrier portion included in the laboratory animal holding device of the present invention. The removable shutter may have a stopper. The stopper fixes the barrier portion to the main body portion, and prevents the barrier portion from vibrating or being detached from the main body portion when the experimental animal runs wild. The type of the stopper is not limited as long as the barrier portion can be fixed to the main body portion, and a projection or a clip can be exemplified. The stopper shown in FIG. 4 is in the form of a projection which engages the gap of the body to secure the barrier to the body.

  FIG. 5 shows a head storage in the laboratory animal holding device of the present invention. The shape of the head storage part is not particularly limited as long as the head of the experimental animal can be stored, but is preferably substantially cylindrical or approximately hemispherical. The head storage unit can be provided with a recess for storing the head of the experimental animal.

  6 and 7 show a perspective view and a front view, respectively, of one aspect of the main body used in the laboratory animal holding device of the present invention. In FIG. 7, the portion enclosed by the broken line is the side edge of the main body portion located on the base side, and the slits of the barrier portion start from the side edge of the main body portion located on the base side . The slit of the barrier portion preferably has a side edge portion of the main body portion corresponding to a position rotated 90 degrees to 270 degrees from the gap portion around the penetration portion center 221, more preferably 135 degrees to 225 degrees from the gap portion More preferably, the main body side edge side corresponding to the position rotated 150 degrees to 210 degrees from the gap portion is set as the start point. Most preferably, the slit of the barrier portion starts from the side edge portion of the main body portion facing approximately 180 degrees from the gap portion.

  Figures 8-10 show a rat retention device. The details of each part are the same as those of the mouse holding device.

  In the present specification, experimental animals mean small animals having a long tail, such as mice and rats, which are used for animal experiments. The holding device of the present invention can also be used when a laboratory animal is reared for companion animals rather than for animal experiments and treated.

  5-30 cm is preferable as a length of the longitudinal direction of a main-body part, and, as for the dimension of the holding | maintenance apparatus for laboratory animals of this invention, 8-25 cm is more preferable. When the restraint apparatus for laboratory animals of the present invention is for rats, the dimension thereof is preferably 5 to 15 cm, more preferably 8 to 12 cm, as the length in the longitudinal axis direction of the main body. When the holding device for laboratory animals of the present invention is for rats, the dimension thereof is preferably 10 to 30 cm, more preferably 15 to 25 cm, as the length in the longitudinal axis direction of the main body.

(Method of operation)
The laboratory animal holding device of the present invention can be used in the following procedure. Although the following procedure is an example of the blood collection procedure from the tail vein, it goes without saying that the experimental animal holding apparatus of the present invention can also be used for blood collection from other than the tail vein and administration of drugs such as anesthesia.
(1) Attach the knob 4 and the head storage 6 to the main body 2 (2) Store the experimental animal from the head in the through portion 22 of the main body 2 (3) Set the barrier 5 (4) Slide the knob 4 and the head storage 6 and hold the experimental animal (5) Collect blood from the tail vein of the tail coming out of the slit 52 (6) When blood collection is completed Remove the knob 4 and the head storage 6 from the main body 2 (7) Take out the end of the main body 2 opposite to the side where the experimental animal is stored from the head

DESCRIPTION OF SYMBOLS 1 Experimental animal holding device 2 Body part 3 Base 4 knob part 5 Barrier part 6 Head storage part 21 Gap part 22 Penetration part 23 Reception part 41 Bolt part 51 Stopper 52 Slit 61 Breathing hole 221 Penetration part center

Claims (9)

A body portion having a penetration portion and a gap portion;
A head storage unit for moving the inside of the penetration portion in parallel with the long axis direction of the main body portion to store the head of the experimental animal;
A knob portion for operating the head storage portion and fixing it to the main body portion;
A barrier with a slit,
A laboratory animal holding device comprising:
The barrier portion is removable from the main body portion, or is attached to the main body portion in an openable / closable manner, and in a state where the barrier portion is attached to the main body portion, the slit is a base The above-mentioned holding device characterized in that it is provided with the side of the main body located on the side as the starting point.   The holding device according to claim 1, wherein the barrier portion is a removable shutter that is removable from the main body portion.   The holding device according to claim 1, wherein the barrier portion further comprises a stopper.   The holding apparatus according to any one of claims 1 to 3, further comprising a base.   The holding device according to claim 4, further comprising a non-slip mechanism on the bottom of the base.   The holding device according to any one of claims 1 to 5, which is for a mouse or a rat.   The holding device according to any one of claims 1 to 6, for use in one-way treatment of experimental animals. Housing the mouse or rat from the head into a holding device;
A one-way treatment method of a mouse or a rat comprising treating a tail of a mouse or a rat and removing the mouse or the rat from the head from the holding device.   9. The method according to claim 8, wherein the treatment is blood collection from a tail vein or artery, drug administration, blood pressure measurement, or marking.

Images