JP2019070632A - Method of evaluating health properties and use thereof - Google Patents

Abstract

SOLUTION: A method of evaluating health properties includes the steps of measuring at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in seminal plasma, and evaluating the health properties of a donor on the basis of concentrations of the at least one component in the seminal plasma.SELECTED DRAWING: None

Description

  The present specification relates to a method of evaluating health characteristics and use thereof.

  Semen is composed of testis, epididymis, vas deferens, seminal vesicles, prostatic secretions (semi- sic plasma), etc. and sperm. Although semen volume, sperm count, sperm concentration, sperm motility rate and shape have been measured in past semen tests (Patent Document 1), most of the biochemical tests of the molecules that make up seminal plasma are performed It is the fact that there is not.

JP, 2017-161301, A

  However, it is believed that seminal plasma plays an important role in imparting fertility to sperm by maintaining the pH of semen constant, promoting sperm motility, stabilizing sperm chromatin, removing active oxygen, etc. Be In addition, seminal plasma contains prostatic fluid, which can be expected to be a biomarker for inflammation of the prostate, hypertrophy and cancer.

  The present specification provides a method that can use semen to evaluate health characteristics, for example, health characteristics such as fertility level and aging level.

  The present inventors focused on seminal plasma that constitutes semen, which is a sample unique to men. Then, by using seminal plasma as a measurement target, it has been found that the health condition as the seminal plasma provider, in particular, the health condition unique to men and the like can be easily evaluated. According to the present specification, the following means are provided.

It is a figure which shows the relationship between the zinc concentration in seminal plasma, and aging. It is a figure which shows the relationship between the zinc concentration in seminal plasma, and a total sperm count. It is a figure which shows the relationship between the zinc concentration in seminal plasma, and the WHO standard of fertility. It is a figure which shows the relationship between the zinc concentration in seminal plasma, and a eating tendency. It is a figure which shows the relationship between testosterone concentration in seminal plasma, and aging. It is a figure which shows the relationship between testosterone concentration in seminal plasma, and the amount of total zinc. It is a figure which shows the relationship between the 8-OHdG density | concentration in seminal plasma, and aging. It is a figure which shows the relationship between 8-OHdG density | concentration in seminal plasma, and a lifestyle (sleeping time). It is a figure which shows the relationship between the creatinine concentration in seminal plasma, and aging. It is a figure which shows the relationship between the zinc concentration in seminal plasma, and the abstinence period. It is a figure which shows the relationship between the zinc concentration in seminal plasma, and the spermine concentration in seminal fluid. It is a figure which shows the relationship between 8-OHdG density | concentration in seminal plasma, and BMI. It is a figure which shows the relationship between BMI and sperm concentration. It is a figure which shows the relationship between testosterone concentration in seminal plasma, and a drinking tendency. It is a figure which shows the relationship between the zinc concentration in seminal plasma, and a lifestyle (smoking). It is a figure which shows the relationship between the concentration of 8-OHdG in seminal plasma, and lifestyle (smoking).

  The disclosure of the present specification relates to a method of evaluating health characteristics, use thereof and the like. According to the method for evaluating a health characteristic disclosed in the present specification (hereinafter also referred to as the present evaluation method) and the evaluation system, the level of fertility, the level of aging can be obtained by acquiring the concentration of a specific component in seminal plasma. Health characteristics such as oxidative stress levels and masculinity levels can be assessed.

  For example, semen contains sperm that can be obtained non-invasively as compared to blood etc, and is directly related to fertility levels. Thus, the concentration of specific components in seminal plasma may more directly reflect male-specific health characteristics such as fertility levels. It is also believed that the concentration of specific components in seminal plasma may reflect the systemic aging levels, oxidative stress levels and virility levels of seminal plasma donors. From these facts, it is possible to evaluate male health characteristics with high sensitivity and accuracy by measuring at least one concentration selected from the group consisting of zinc, testosterone, oxidative stress marker and creatine in seminal plasma. In particular, by measuring the concentration of specific components in seminal plasma, the health characteristics (semen age) of seminal plasma donors' semen can be examined in various aspects, such as fertility level, aging level, oxidative stress level and femininity level. From the point of view, it can be easily evaluated.

  For example, it was found that the higher the concentration of zinc and testosterone in seminal plasma, the higher the total sperm count (the total number of sperm contained in semen per ejaculation). Conventionally, motility and morphology of sperm in semen have been associated with fertility, but according to this evaluation method, even if conventional measurement items such as sperm count and semen volume are omitted, semen The level of fertility can be easily evaluated from the concentration of zinc and testosterone in serum.

  It was also found that the concentrations of testosterone and creatine in seminal plasma tend to decrease as the donor's age increases. From this, according to the seminal plasma concentration of one or two components selected from these three components, it is possible to easily evaluate the aging level and the masculinity level of the donor.

  It was also found that oxidative stress markers in seminal plasma tend to increase as the donor's age increases. From this, the seminal plasma concentration of this component can be used to assess the aging level of the donor. In addition, since the oxidative stress marker itself indicates the oxidative stress level, the oxidative stress level of the donor can be evaluated.

  Furthermore, according to the present specification, there is also provided a health support method and system for acquiring health support information of a seminal plasma donor based on evaluation results of health characteristics based on the concentration of a specific component in seminal plasma. Hereinafter, various embodiments of the present evaluation method and the use thereof will be described.

(How to evaluate health characteristics)
The method for evaluating a health characteristic disclosed herein comprises the step of measuring at least one component selected from the group consisting of testosterone, zinc, a stress marker and creatine in seminal plasma (hereinafter referred to as seminal plasma component (Also referred to as a measurement step). By providing such a step, the health characteristics of the seminal plasma donor can be easily evaluated based on the seminal plasma concentration.

  The measurement object of this evaluation method is seminal plasma. Seminal plasma is the remaining component of sperm obtained by removing sperm. Seminal plasma includes, for example, seminal vesicles, prostate and the like. Although seminal plasma has not conventionally been focused on fertility and the like, according to the present inventors, it leads to acquiring the relationship between a specific component in seminal plasma and the level of pregnancy, etc. We learned the usefulness of this as a measurement target.

  The present evaluation method measures seminal plasma, which is a part of semen as described later. Therefore, semen is usually used as a sample, and sperm is removed from the semen to separate seminal plasma prior to the measurement step. In addition, even in the presence of sperm, if it is possible to measure a specific component, seminal plasma in the presence of sperm (that is, semen) can also be a measurement target. In that case, in the present specification, the seminal plasma concentration can be replaced with the seminal fluid concentration.

  Seminal plasma can be obtained as supernatant obtained by centrifuging semen, or as semen, as filtrate obtained by filtration with a filter that does not pass sperm. Therefore, when semen is obtained as a sample and seminal plasma is to be measured, the process of removing sperm from semen to obtain seminal plasma may be appropriately performed prior to or as part of the measurement process. it can.

(Measurement process of components in seminal plasma)
The seminal plasma component can be at least one selected from the group consisting of testosterone, zinc, a stress marker and creatine. Among such components, preferably 2 or more, more preferably 3 or more, and still more preferably 4 or more.

(testosterone)
Testosterone is a type of steroid hormone that belongs to androgen. Testosterone can be measured by a known method, but in consideration of convenience, accuracy and the like, it is preferable to measure using an antigen-antibody reaction. For example, an ELISA method using anti-testosterone antiserum and anti-testosterone antibody can be employed. Kits for performing such ELISA methods are readily commercially available.

  Seminal plasma testosterone concentration is thought to reflect testosterone levels in seminal plasma donors. Therefore, it is useful for evaluation of male function (male menopausal disorder, sexual dysfunction, etc.). It is also disclosed herein that testosterone concentration in seminal plasma decreases with age, and increasing same testosterone concentration increases total sperm count and fertility levels. Therefore, seminal plasma testosterone concentration is useful for the assessment of aging levels, total sperm count, fertility levels of seminal plasma donors.

(zinc)
Zinc is a metal atom. The measurement of zinc is not particularly limited. For example, x-ray fluorescence method, atomic absorption method, 5-Br-PAPS (5-Br-PAPS: 2- (5-bromo-2-pyridylazo) -5-) It can be measured by a color method using (N-propyl-N-sulfopropylamino) phenol sodium) or the like.

  Seminal plasma zinc concentration is thought to reflect zinc levels in seminal plasma donors. Therefore, it is useful for evaluation of male function (male menopausal disorder, sexual dysfunction, etc.). It is also disclosed herein that the concentration of zinc in seminal plasma decreases with age, and increasing the concentration of zinc increases total sperm count and fertility levels. Therefore, the zinc concentration in seminal plasma is useful for evaluating the aging level, total sperm count, and fertility level of seminal plasma donors.

(Oxidative stress marker)
An oxidative stress marker is a marker that uses oxidation of a metabolite in a living body as an indicator, and is generally a substance that serves as an indicator of oxidative stress in a living body. Various markers are known as oxidative stress markers. Although not particularly limited, examples of oxidative stress markers include DNA oxidation markers such as 8-hydroxy-2'-deoxyguanosine (8-OHdG), 8-OHdG, hexanoridin (HEL), 4-HNE (4 -Hydroxynoneneal), isoplastan, malondialdehyde, lipid peroxide, lipid oxidation marker such as oxidized LDL, carboxymethyl lysine, glycated albumin, bentosidine, glycoglycation marker such as methylglyoxal, deoxyglucosone, dityrosine, Examples include protein oxidation markers such as carbonylated proteins, NO stress markers such as nitrotyrosine, and the like. Among them, 8-OHdG is preferable as an oxidative stress marker in seminal plasma.

  Although any oxidative stress marker such as 8-OHdG is commercially available and can be measured by a known method, in consideration of convenience, accuracy, etc., measurement should be performed using an antigen-antibody reaction. Is preferred. For example, in the case of 8-OHdG, an ELISA method using an anti-8-OHdG antiserum and an anti-8-OHdG antibody can be adopted. Kits for performing such ELISA methods are readily commercially available.

  Oxidative stress marker concentrations such as 8-OHdG in seminal plasma are thought to reflect oxidative stress levels in seminal plasma donors. Therefore, it is useful for evaluation of male function (male menopausal disorder, sexual dysfunction, etc.). Also disclosed herein is that the concentration of 8-OHdG in seminal plasma increases with age, and increasing the concentration of 8-OHdG decreases the concentration of testosterone. Thus, the concentration of 8-OHdG in seminal plasma is useful for assessing the aging level of seminal plasma donors.

(Creatine)
Creatine is 1-methyl guanidinoacetic acid (or methyl glycosamine). Creatine is converted to creatine phosphate by the action of creatine kinase, is important as a muscle energy source, and is considered useful for muscle hypertrophy and the like.

  Creatine can be measured by a known method, but in consideration of convenience, accuracy and the like, the chromogenic method is preferred. The color development method is not particularly limited, but for example, a color development method by an enzyme method can be used. Kits for performing such color development methods are readily commercially available.

  In the present evaluation method, one or two or more of these components can be measured. Creatine levels in seminal plasma are thought to reflect creatine levels in seminal plasma donors. It is disclosed herein that the concentration of creatine in seminal plasma decreases with age. Thus, seminal plasma creatine concentration is useful for assessing the aging level of seminal plasma donors.

  Among these components, according to the present evaluation method, it is preferable to measure the zinc concentration. By measuring the zinc concentration, it is possible to evaluate the level of fertility of the seminal plasma donor without, among other things, measuring the total sperm count or the amount of semen.

  Moreover, among these components, it is preferable to measure the testosterone concentration and the creatine concentration. These are useful for assessing the aging level of seminal plasma donors.

  Further, among these components, it is preferable to measure the testosterone concentration. High testosterone levels increase total sperm count and are useful for assessing the level of fertility in seminal plasma donors.

  In addition, it is preferable to measure the concentration of an oxidative stress marker such as 8-OHdG in that it may reflect lifestyle habits such as sleeping time. In addition, it is preferable to measure the zinc concentration in that it may reflect the tendency of the diet (the tendency to eat a large amount of meat, the tendency to a large amount of fish).

  It is also preferable to measure the testosterone concentration and the concentration of an oxidative stress marker such as 8-OHdG. Testosterone levels decrease with age, and 8-OHdG levels increase with age. Therefore, by measuring both, it is possible to evaluate the aging level of the seminal plasma donor with high accuracy. In addition, testosterone concentration correlates with total sperm count, ie, fertility level, and oxidative stress marker concentration such as 8-OHdG is an indicator of oxidative stress of seminal plasma which is a sperm-containing environment, so sperm-containing environment Oxidative stress levels can be assessed.

(Spermin)
Spermine is one of the substances involved in cell division and protein synthesis. It is thought to be involved in cell metabolism.

(Evaluation process of health characteristics)
The evaluation method may comprise the step of evaluating the health characteristics of the donor of seminal plasma based on the seminal plasma concentration of at least one component. The evaluation target in the evaluation step may be the seminal plasma concentration of all the components measured in the measurement step, or may be part thereof. The seminal plasma concentration to be evaluated is appropriately determined according to the intention of the seminal plasma donor or the health characteristics targeted by the evaluation method.

  The evaluation step evaluates the health characteristics of the seminal plasma donor, for example, by comparing the acquired seminal plasma concentration with a reference concentration associated with a specific health characteristic previously acquired for the component. Can. The reference concentration is set for one or more components in association with at least one of the health characteristics selected from the group consisting of aging level, fertility level and oxidative stress level.

(zinc)
For example, according to the present invention, since the concentration of zinc in seminal plasma is strongly correlated with the total sperm count, the total sperm count in semen, which is the standard of fertility determined by WHO (World Health Organization) The reference concentration of zinc in seminal plasma can be determined to be (3.9 × 10 ⁷ cells or more). The seminal plasma zinc concentration that can be positively evaluated as having WHO standard fertility is not particularly limited, and is, for example, 9.4 mg / dl or more, and, for example, 9. It is 5 mg / dl or more, for example, 10 mg / dl or more, for example, 12 mg / dl or more, and for example 14 mg / dl or more. In addition, it is preferable that all these density | concentrations depend on the measuring method of the precision method or the precision more than the color method by 5-Br-PAPS, or the said method or more.

(testosterone)
Also, for example, according to the present specification, testosterone concentration in seminal plasma is strongly correlated with the number of total sperm in semen, and therefore semen serving as the standard of fertility determined by WHO (World Health Organization) A reference concentration of testosterone in seminal plasma can be determined which results in a total sperm count (> 3.9 × 10 ⁷ cells). By comparing the testosterone concentration in the seminal plasma obtained by the measurement process with the reference concentration, whether or not fertility is high, fertility is high or the ratio to the WHO standard (for example, 120% etc.), low or the WHO standard The level of fertility of the seminal plasma donor can be assessed, which is a degree such as a percentage of (eg, 90%, etc.).

  The reference concentration of testosterone in seminal plasma can be varied or changed as appropriate depending on the change in WHO standard, the increase in fertility and the number of data to be set, and the like.

  Also, for example, according to the present specification, testosterone concentration in seminal plasma decreases with age and inversely correlates with aging, so that seminal plasma is associated with a reference age such as each age, each age, etc. It is possible to set a reference concentration of testosterone in it. By comparing the testosterone concentration in seminal plasma obtained by the measurement step with the reference concentration, the seminal plasma-based age of the seminal plasma donor is younger than the actual age, which is equivalent to the real age of the donor, or minus The level of aging can be assessed, such as how old you are, or who is older than the actual age or plus how old.

  The reference concentration of testosterone in seminal plasma can be varied due to the increase in the number of data, or can be set appropriately. The reference concentration is not particularly limited, and is, for example, 2150 pg / ml in 20's, for example 1500 pg / ml in 30's, and for example, 1500 pg / ml in 40's. Also, for example, it is 1350 pg / ml at the age of 50. In addition, it is preferable that all these density | concentrations depend on the measuring method of the precision and accuracy more than an ELISA method or the said method, or more.

  Also for example, according to the present invention, the concentration of testosterone in seminal plasma is itself an indicator of the testosterone level of the seminal plasma donor, ie the level of maleness. Therefore, the testosterone concentration in seminal plasma can be associated with a reference age such as each age and each age to determine the reference concentration of oxidative stress marker in seminal plasma. The oxidative stress based on the seminal plasma of the seminal plasma donor is equivalent to the real age of the donor by comparing the oxidative stress marker concentration in seminal plasma obtained by the measurement step with the reference concentration of the actual age. It can be evaluated as the percentage of actual age to oxidative stress (%) or the actual age plus what age or minus what age.

(Oxidative stress marker)
Also, for example, according to the present specification, since the concentration of oxidative stress markers such as 8-OHdG in seminal plasma increases with age and is correlated with aging, criteria such as each age, each age, etc. A baseline concentration of oxidative stress markers such as 8-OHdG in seminal plasma can be determined in relation to age. Aging levels are evaluated in the same manner as in the case of testosterone concentration for the age based on seminal plasma of seminal plasma donors by comparing the oxidative stress marker concentration in seminal plasma obtained by the measurement process with the reference concentration can do.

  The reference concentration of 8-OHdG in the seminal plasma can fluctuate due to the increase of the number of data, etc., and can fluctuate due to the increase of the number of data, etc. Such a reference concentration is not particularly limited, for example, 17 ng / ml at 20's, 18 ng / ml at 30's, and for example, 22 ng / ml at 40's For example, it is 26 ng / ml at the age of 50. In addition, it is preferable that all these density | concentrations depend on the measuring method of the precision and accuracy more than an ELISA method or the said method, or more.

  Also for example, according to the present invention, the concentration of oxidative stress markers such as 8-OHdG in seminal plasma is itself also an oxidative stress level of seminal plasma donors, and also of oxidative stress levels on spermatozoa in seminal fluid It also serves as an indicator. Therefore, it is also possible to set and evaluate an oxidative stress marker concentration such as 8-OHdG in seminal plasma in association with fertility and the like. For example, reference concentrations of oxidative stress markers in seminal plasma can be determined in association with reference ages such as each age and each age. The oxidative stress based on the seminal plasma of the seminal plasma donor is equivalent to the real age of the donor by comparing the oxidative stress marker concentration in seminal plasma obtained by the measurement step with the reference concentration of the actual age. It can be evaluated as the percentage of actual age to oxidative stress (%) or the actual age plus what age or minus what age.

(Creatine)
Also, for example, according to the present specification, since creatine concentration in seminal plasma decreases with age and inversely correlates with aging, seminal plasma is associated with a reference age such as each age, each age, etc. A reference concentration of creatine in can be determined. By comparing the creatine concentration in the seminal plasma obtained by the measurement step with the reference concentration, the seminal plasma age of the seminal plasma donor is younger than the actual age, which is equivalent to the real age of the donor, or minus The level of aging can be assessed, such as how old you are, or who is older than the actual age or plus how old.

  The reference concentration of creatine in seminal plasma can be varied by increase of the number of data, etc., or can be set appropriately, for example, it is 0.65 nmol / μl at 20 years old, and for example, at 30 years old. It is 0.45 nmol / μl, and it is, for example, 0.4 nmol / μl at the age of 40, and for example, 0.4 nmol / μl at the age of 50. In addition, it is preferable that all these density | concentrations depend on the coloring method by an enzyme method, or the measuring method of the precision and accuracy equivalent or more than the said method.

  Also for example, according to the present invention, the concentration of creatine in seminal plasma is itself also the creatine level of the seminal plasma donor, but the level is also an indicator of the masculinity level. Therefore, the creatine concentration in seminal plasma can be related to the reference age such as each age and each age as described above to determine the reference concentration of creatine in seminal plasma. By comparing the creatine concentration in the seminal plasma obtained by the measurement step with the reference concentration of the actual age, the masculinity level based on the seminal plasma of the seminal plasma donor is equivalent to the actual age of the donor. It can be evaluated as the ratio (%) of the age to the masculinity level or the actual age plus what age or minus how old.

(Spermin)
Also, for example, according to the present specification, since spermine concentration in seminal fluid is positively correlated with zinc concentration in seminal plasma, setting a concentration as a standard of fertility as in zinc in seminal plasma Can. In addition to spermine, liquid chromatography methods (detection: ESI-MS, ESI-MS / MS, fluorescence, etc.) described in the examples are possible, and in addition to general HPLC methods, LC-MS method, LC -MS / MS method, ELISA method, color method can be mentioned.

  In the above description, for all of the fertility level, aging level, oxidative stress level and masculinity level, for example, the age of the subject who obtained the seminal plasma concentration of these components or the fertility standard of WHO in advance. As another evaluation mode, for example, target fertility level (zinc concentration or total sperm count) or target for each subject of the evaluation method who is a seminal plasma donor Concentrations corresponding to the age level, oxidative stress level and masculinity level of the age or age to be taken may be used as a reference value for evaluation. That is, evaluation may be made by comparing the seminal plasma concentration obtained by measurement with a reference value which is a preset target value. By doing this, for example, motivation for health promotion can be given.

  Further, in the above description, the evaluation process is described as comparing with a preset reference value, but it is not limited to this. For example, the pregnancy level, aging level, oxidative stress level and masculinity level Changes over time in the test subject, that is, the measured value of the subject's own past, recent, or a certain period of time (for example, one month, three months, six months, or one year) before the reference value It may be a step of evaluating a tendency (such as improvement, maintenance, improvement or decline).

  In the evaluation step, it is possible to evaluate, for example, one or more levels meeting the purpose and the like from among the fertility level, the aging level, the oxidative stress level and the manly level. As male-specific health characteristics, it is preferable to evaluate at least the level of fertility, and the level of masculinity can also be evaluated as appropriate. Further, the aging level and the oxidative stress level are suitable for comprehensively evaluating male-specific health characteristics, and also useful for so-called health evaluation.

  In the evaluation step, evaluation of one or more levels selected from the group consisting of fertility level, aging level, oxidative stress level and masculinity level is integrated, for example, male as semen age as a comprehensive evaluation It may be evaluated as an intrinsic health characteristic. For example, evaluation results for one or more levels can be made into points by a five-step evaluation method or the like, and a total of these points or a total after giving appropriate coefficients can be comprehensively evaluated. .

  In addition, individual levels may be individually evaluated by making points or the like as necessary, with or without such comprehensive evaluation.

  This evaluation method is about the semen collected by the evaluation organization concerned or the hospital etc. besides execution in the aspect which provides the evaluation organization which implements this evaluation method by mail etc. which semen collected not at a hospital etc. but at home etc. It is possible to implement in the aspect etc. which evaluate in the said place. In particular, the present evaluation method is useful in that it has a high degree of freedom and selectivity for collection, and can provide a sample without facing a medical worker.

  In addition, this evaluation method can be configured such that the subject can individually obtain evaluation results in a mail, mail, or a personal account provided on a specific website. That is, the present evaluation method can receive evaluation at a more personal and arbitrary timing by including the step of outputting the evaluation result to the medium so as to ensure secrecy.

  In this evaluation method, for example, zinc concentration in seminal plasma, oxidative stress marker concentration such as 8-OHdG, testosterone even after ejaculation, for example, after 1 hour and 7 days at 15 ° C. to 35 ° C. Concentrations and creatine concentrations can be measured with high accuracy and accuracy.

  This evaluation method is a test item that requires fresh semen, such as the amount of semen, total sperm count, sperm motility, sperm count, and sperm morphology, and simplifies complicated and skillful measurement required. Even if omitted and implemented, health characteristics can be sufficiently evaluated.

(Health support method)
The health support method (hereinafter simply referred to as the present support method) disclosed herein comprises at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in seminal plasma. Measuring, evaluating a health characteristic of a donor of the seminal plasma based on the seminal plasma concentration of the at least one component, acquiring health support information based on an evaluation result of the health characteristic, and , Can be provided. According to this support method, health support information of the seminal plasma provider can be obtained based on the evaluation result of the health property, so that the health property of the seminal plasma provider can be improved, maintained or improved.

  The component measurement step and the evaluation step in the present support method can be performed in the same manner as the measurement step and the evaluation step in the present evaluation method.

(Health support information acquisition process)
The health support information providing step is a step of acquiring health support information based on the evaluation result of the health characteristic. The evaluation result of the health characteristic is one or more selected from the group consisting of the level of fertility, the level of aging, the level of oxidative stress and the level of masculinity of the seminal plasma donor. Which evaluation result is to be used to provide health support information is appropriately set according to the health purpose of the subject.

  The health support information based on the evaluation result of the health characteristic can be information that can improve, maintain or improve one's own health characteristic based on the evaluation result of the health characteristic. Examples of the type of the support information include, for example, nutrition related information on nutritional components to be ingested, lifestyle related information on lifestyle (sleep, exercise and the like), and the like.

  The nutrition related information includes, for example, contents suggesting start or increase of zinc intake when fertility levels are insufficient or tend to be lowered due to insufficient or low concentration of zinc in seminal plasma, etc. Can. More specifically, there are contents promoting zinc-containing supplements and foods containing a large amount of zinc. Also, according to the present specification, it is disclosed that the more the meat eats, the higher the concentration of zinc in seminal plasma. For this reason, for example, as nutrition related information, contents such as increasing meat dishes and main contents can be included.

  Also, for example, as nutrition related information, when the creatine concentration and testosterone concentration in seminal plasma are insufficient or decreasing tendency and the aging level is negative or decreasing than actual age, intake of creatine is started or It can include content suggesting an increase. More specifically, creatine-containing supplements and the like and contents promoting promotion of food containing a large amount of amino acids can be mentioned. Further, it may include contents proposing the initiation or increase of the intake of an antioxidant supplement that is generally considered to reduce oxidative stress. Furthermore, it can include content that arouses the need for examination of testosterone replacement therapy.

  Also, for example, when the concentration of oxidative stress markers such as 8-OHdG in seminal plasma is increasing or increasing, and the aging level is negative or decreasing than the actual age (or the oxidative stress level is negative or increasing) Etc.) may include content suggesting the initiation or increase of the intake of antioxidant supplements. Further, according to the present specification, the longer the sleeping time, for example, preferably 5 hours or more, more preferably 6 hours or more, still more preferably 7 hours or more, and still more preferably 8 hours or more. It is disclosed that the 8-OHdG concentration of For this reason, for example, as lifestyle-related information, it is possible to include contents such as extending sleep and promoting rest.

  Such health support information is a database of health support information associated with the evaluation results obtained, for example, the seminal plasma concentration of various components, the point converted from seminal plasma concentration of various components according to a certain standard, and the comprehensive points. For example, it can be stored in advance in control means such as a computer, an internal memory, an external memory or a cloud. For example, regarding fertility levels, if the subject's seminal plasma zinc concentration is lower by a certain percentage from the standard value, it will be taken as nutrition related information such as increasing the meat mass, and if lower, intake of zinc supplement etc. is started It is possible to associate and store nutrition related information such as, etc.

  When such a memory or the like is prepared, appropriate health support information can be obtained by accessing a computer or cloud provided with such a memory or the like and inputting an evaluation result, and the health support information can be Can be provided.

  When providing health support information to a subject, it can be provided in various modes. The form of provision is not particularly limited, and is appropriately selected. For example, paper output by a printer, output by mail, output via a server or a cloud, and the like can be mentioned.

  In addition, the evaluation method of the health characteristic disclosed by this specification can also be implemented as an evaluation system of the health characteristic, for example. For example, the health property evaluation system measures seminal plasma separation means for separating seminal plasma from seminal fluid, and at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in the seminal plasma. And means for evaluating the health characteristics of the donor of the semen based on the seminal plasma concentration of the at least one component. In such a system, the seminal plasma separation means is a known centrifugal device, the measurement means is a device or kit for measuring at least one of the components, and the evaluation means is, for example, at least one of the components A computer with a control unit (control unit) that evaluates the seminal plasma concentration obtained for the health properties, that is, health properties such as fertility level, aging level, oxidative stress level and masculinity level, etc. It can be done. In the computer or the like, reference concentration information on the reference concentration of various components associated with the specific health characteristic can be provided as a database in the computer or the cloud. The control unit accesses the reference concentration information database to compare the seminal plasma concentration of at least one type of component obtained from the subject with the reference concentration, etc. Acquisition, i.e. the health characteristics of the subject can be assessed.

  Such a health property evaluation system can also be configured as a health property evaluation device. For example, the concentration in the seminal plasma of the at least one component obtained from a centrifugal separator, a measuring device for at least one component, and a measurement result (for example, coloration etc.) by the measuring device is calculated. And a reference concentration to be compared in the reference concentration information database, and can be configured as a device including control means for acquiring the health characteristics of the subject, and means for outputting the health characteristics as needed.

  Furthermore, the health support method disclosed herein comprises at least one selected from the group consisting of seminal plasma separation means for separating seminal plasma from semen, testosterone in seminal plasma, zinc, an oxidative stress marker and creatine. And means for evaluating the health characteristics of the donor of the seminal plasma based on the seminal plasma concentration of the at least one component, and health based on the evaluation results of the health characteristics. A health support information acquisition unit that acquires support information can be configured as a health support system. Such a system can be, for example, the above-mentioned seminal plasma separation means, the above-mentioned measurement means, and the above-mentioned evaluation means similar to the health characteristic evaluation system, and the above-mentioned health support information acquisition means It is possible to obtain health support information such as nutrition related information and lifestyle related information by accessing the database of the above-mentioned health support information associated with the evaluation result obtained by the above.

  Such a health support system can also be configured as a health support information acquisition device. For example, the concentration in the seminal plasma of the at least one component obtained from a centrifugal separator, a measuring device for at least one component, and a measurement result (for example, coloration etc.) by the measuring device is calculated. And control means for comparing the reference concentration to be compared in the reference concentration information database to obtain the health characteristics of the subject, and further obtaining health support information based on the evaluation result of the health characteristics, and as necessary And means for outputting the health support information.

  Hereinafter, specific examples will be described to more specifically explain the disclosure of the present specification. The following examples are for the purpose of illustrating the disclosure of the present specification and are not intended to limit the scope thereof.

  In the following example, 80 healthy male subjects aged 23 to 58 were requested to collect a sample (semen) at home. Dante Co., Ltd. received the collected samples and the questionnaire on the diet and lifestyle habits, and conducted an examination on the following items.

(1) Measurement of semen volume The semen weight was measured for each collection container, and the semen weight was calculated by reducing the empty container weight. The specific gravity of semen was set to 1 and converted to semen volume.
(2) Sperm Concentration The number of spermatozoa was measured using a modified Neubauer hemacytometer, whereby the sperm concentration was measured.
(3) Zinc Concentration in Seminal Plasma Semen was centrifuged at 800 g for 7 minutes, and the supernatant was obtained as seminal plasma. The zinc in seminal plasma is chelated with 5-Br-PAPS (2- (5-bromo-2-pyridylazo) -5- (N-propyl-N-sulfopropylamino) phenol sodium) to form a chelate complex. The color of the visible part of the complex was measured and quantified. For measurement, a metalloassay zinc measurement LS kit (manufactured by Metallogenics Co., Ltd.) was used, and the protocol of the kit was followed.
(4) Seminal plasma testosterone concentration Seminal plasma was obtained in the same manner as (3), and the testosterone concentration was measured by the immunoantibody method using an anti-testosterone antibody. For the measurement, testosterone, ELISA kit (strip plate) (manufactured by IBL Inc.) was used, and the protocol of the kit was followed.
(5) Concentration of 8-OHdG in Seminal Plasma Sperm was centrifuged at 1500 g for 5 minutes to obtain a supernatant as seminal plasma. 8-OHdG in seminal plasma was measured by an immunoantibody assay using an anti-8-OHdG antibody. The measurement was according to the following method.

<Production of protein chip>
The 8-OH dG-BSA prepared according to a conventional method was diluted to 5 μg / mL to make a spot solution. As a glass substrate, an azo slide manufactured by Sakai Corporation was used. Using a Spotter SHOTMASTER 300DS-s manufactured by Musashi Engineering, 160 spots of 36 nL were spotted per azo slide, and blue light was applied to immobilize them to prepare a protein chip.

<Measurement with protein chip>
3 μL of a biotinylated anti-8-OHdG antibody (1.33 μg / mL) and 6 μL each of a 4-fold diluted seminal plasma sample and a serially diluted standard solution were respectively mixed, and shaken for 15 minutes. 4.7 μl of this mixture was added dropwise to the “antibody chip” and incubated under wet conditions for 1 hour. The “antibody chip” was washed twice with TPBS (Tween 200 0.01 w / v% phosphate buffered saline), and then water was removed by centrifugation.

  The "protein chip" was immersed in a solution of alkaline phosphatase labeled streptavidin (manufactured by KPL) diluted 1000-fold with TPBS, and shaken for 1 hour. The “protein chip” was washed twice with TPBS, rinsed with phosphate buffered saline (PBS), and water was removed by centrifugation.

The luminescence was detected for 30 minutes using Tropix CDP-Star (manufactured by Roche) as a luminescence substrate and Luminograph I (manufactured by Atto) as a detector. A calibration curve was prepared from the detection values of the standard, and the 8-OHdG value of the seminal plasma sample was calculated.
(6) Serum Creatine Concentration A seminal fluid sample (26 samples (age 24 to 58) indiscriminately extracted) was centrifuged at 12,000 rpm for 30 minutes to obtain a supernatant as seminal plasma. The creatine concentration was measured by the following method: For measurement, use the Creatine Colorimetric / Fluorometric Assay Kit (manufactured by BioVision), and use the serum plasma prepared by diluting the above-mentioned seminal plasma by a factor of 16 and the standard solution prepared by serial dilution on a microplate (manufactured by 50 μL aliquots of each of the wells (5), 50 μL aliquots of the color solution from the kit were dispensed into the above wells, and incubated for 60 minutes at 37 ° C. Thereafter, using a microplate reader (INFINITE M1000 Pro, manufactured by Tecan), the wavelength was adjusted. The absorbance was measured at 570 nm, and a standard curve was prepared from the detection values of the standard. It was calculated creatine concentration.

(Result of analysis on seminal plasma concentration of various components)
The analysis results using the above various seminal plasma concentrations are shown in FIGS.

(Zinc concentration in seminal plasma)
The analysis result about the zinc concentration in seminal plasma is shown in FIGS. FIG. 1 shows the relationship between subject's age and seminal plasma zinc concentration. As shown in FIG. 1, it was found that the zinc concentration in seminal plasma was not related to the age. Further, FIG. 2 shows the relationship between the total sperm count in seminal fluid and the zinc concentration in seminal plasma. As shown in FIG. 2, it was found that the total sperm count increased as the zinc concentration increased. Furthermore, FIG. 3 shows the concentration of zinc in the seminal plasma of the samples that meet and do not meet the total sperm count of 3.9 × 10 ⁷ cells, which is the standard of fertility in WHO. The result of having compared the average value is shown. As shown in FIG. 3, the zinc concentration in seminal plasma was 14.6 mg / ml or more in the WHO standard satisfaction group, and the same concentration was 9.4 mg / ml in the WHO standard non-satisfaction group.

  Further, FIG. 4 shows the relationship between the carnivores in the diet, that is, whether it is meat-centered or fish-centered, and the zinc concentration in seminal plasma. As shown in FIG. 4, it was found that the zinc concentration in seminal plasma was higher as the meat was eaten more. The diet was a trend for 7 days before the sample collection.

  From the above results, it has been found that the zinc concentration in seminal plasma is a good indicator of the total sperm count and, for example, a good indicator of fertility (fertility level) represented by the WHO standard. Also, it has been found that by appropriately determining the standard of fertility level such as, for example, the total sperm count, the seminal plasma zinc concentration against the standard can be used as a standard value of fertility level. In addition, since the concentration of zinc in seminal plasma also changes depending on the content of the diet, it is understood that lifestyle advice for improving the level of fertility can be made in order to increase the concentration of zinc in seminal plasma, in other words The

(Testosterone concentration in seminal plasma)
The analysis results of testosterone concentration in seminal plasma are shown in FIGS. FIG. 5 shows the relationship between the subject's age and the concentration of testosterone in seminal plasma. As shown in FIG. 5, it was found that the testosterone concentration in seminal plasma decreases with age. Further, FIG. 6 shows the relationship with the total amount of zinc in semen. As shown in FIG. 6, it was found that the total amount of zinc increased as the testosterone concentration increased.

  From the above results, it was found that seminal plasma testosterone concentration is a good indicator of aging level. It has also been found that, for example, testosterone concentration is a good indicator of total zinc content. The total zinc content is also related to sperm fertility, and since testosterone is a male hormone, it has been found that the testosterone concentration in seminal plasma is a good indicator of masculinity.

(Concentration of 8-OHdG in seminal plasma)
The analysis results for 8-OHdG concentration in seminal plasma are shown in FIGS. 7-8. FIG. 7 shows the relationship between the subject's age and the concentration of 8-OHdG in seminal plasma. As shown in FIG. 7, it was found that the concentration of 8-OHdG in seminal plasma increases with age. Further, FIG. 8 shows the relationship between the average sleep time of the subject and the concentration of 8-OHdG in the seminal plasma. As shown in FIG. 8, it was found that the longer the average sleep time, the higher the 8-OHdG concentration, and the shorter the average sleep time, the lower the 8-OHdG concentration. In addition, a meal was taken as the average sleeping time in 7 days before sample collection.

  From the above results, it was found that the concentration of 8-OHdG in seminal plasma is a good indicator of aging level. Also, for example, 8-OHdG concentration was found to be a good indicator of average sleep time. It was found that 8-OHdG is an oxidative stress marker and is a good indicator of oxidative stress level, as is apparent from the relationship with the average sleep time.

(Creatine concentration in seminal plasma)
The analysis result about the creatine concentration in seminal plasma is shown in FIG. FIG. 9 shows the relationship between the subject's age and the concentration of seminal plasma creatine. As shown in FIG. 9, it was found that the concentration of seminal plasma creatine decreases with age.

  From the above results, it was found that seminal plasma creatine concentration is a good indicator of aging level. In addition, for example, since creatine itself is a good indicator of muscle strengthening and regeneration, it has been found that the concentration of seminal plasma creatine is a good indicator of masculinity level.

  In the following example, 194 healthy male subjects in their twenties to sixties were asked to collect a sample (semen) at home. Dante Co., Ltd. received a questionnaire concerning the collected samples and physical condition, etc., and examined the following items (1) to (6). The subject's weight and height were measured in advance to obtain BMI. Questionnaire items included an abstinence period (day) at the time of sample collection, drinking habits and smoking habits.

(1) Measurement of semen volume The same procedure as in Example 1 was performed.
(2) Sperm Concentration The same procedure as in Example 1 was performed.
(3) Concentration of Zinc in Seminal Plasma The same procedure as in Example 1 was carried out.
(4) Concentration of testosterone in seminal plasma Conducted in the same manner as in Example 1.
(5) Concentration of 8-OHdG in seminal plasma Conducted in the same manner as in Example 1.
(6) Spermine concentration in seminal fluid Spermine is a component of seminal fluid and is a substance involved in cell division and protein synthesis. Spermine, J Chromatogr A. And 4- (N, N-dimethylaminosulfonyl) -7-fluoro-2,1,3-benzoxadiazole (DBD-F) described in 2008 Sep 26; 1205 (1-2): 94-102. Measured by UPLC with derivatization used. That is, there is a method in which the deproteinized supernatant obtained by adding methanol to semen and centrifuging at 15000 g for 20 minutes at 4 ° C. is reacted with DBD-F to be derivatized and then analyzed by UPLC. The chromatographic conditions were as follows.
Liquid chromatograph: ACQUITYTM Ultra Performance Liquid Chromatography and Micromass LCT PremierTM XE Mass Spectrometer (High sensitivity orthogonal time-of-flight instrument; Waters, Milford, USA)
Column: ACQUITY UPLC BEH C18 column (1.7 μm, 100 × 2.1 mm id; Waters)
Flow rate: 0.4 mL / min Mobile phase: A; 0.1% formic acid aqueous solution, B; 0.1% formic acid acetonitrile solution (gradient conditions: B% = 20 (0 min)-60 (8 min)-90 (10 Minutes)-98 (11 minutes)-98 (12 minutes)-20 (13 minutes)-20 (20 minutes)
Detection: 550 nm (excitation wavelength: 450 nm)

(Analysis result)
The result of having analyzed about a questionnaire item and each said evaluation item is shown in FIGS. 10-16.

(Zinc plasma zinc concentration and abstinence period)
The analysis results of the abstinence period and the concentration of zinc in seminal plasma are shown in FIG. As shown in FIG. 10, it was found that when the abstinence period increased, the zinc concentration in seminal plasma tended to increase.

(Spermine concentration in seminal fluid and zinc concentration in seminal plasma)
The analysis results of spermine concentration in seminal fluid and zinc concentration in seminal plasma are shown in FIG. As shown in FIG. 11, it was found that when the spermine concentration in seminal fluid is high, the zinc concentration in seminal plasma tends to be high.

(BMI and 8-OHdG concentration in seminal plasma)
The relationship between BMI and 8-OHdG concentration in seminal plasma is shown in FIG. As shown in FIG. 12, when classified into lean type with BMI less than 18.5, ordinary type with 18.5 to 25 and obese type with 25 or more, the higher the BMI, ie, lean type, ordinary type It was found that the concentration of 8-OHdG in seminal plasma was low in the order of obese type, and oxidative stress tended to be low.

(BMI and sperm concentration)
The relationship between BMI and sperm concentration is shown in FIG. As shown in FIG. 13, when classified into lean type with BMI less than 18.5, normal type with 18.5 to 25 and obese type with 25 or more, the higher the BMI, the higher the concentration of zinc in seminal plasma I found that there is. Further, as shown in FIG. 12B, it was found that as the BMI is lower, that is, the sperm concentration tends to be higher in the order of lean, normal and obese types.

(Drinking habit and testosterone concentration in seminal plasma)
The relationship between drinking habit and testosterone concentration in seminal plasma is shown in FIG. The influence of age was adjusted by covariance analysis, as the concentration of testosterone in seminal plasma tends to be lower as the age increases. As shown in FIG. 14, the tendency of testosterone concentration in seminal plasma differs depending on the presence or absence of drinking habit (with: 1 day or more per week, none: not at all), and if there is drinking habit, the testosterone concentration in seminal plasma is high It turned out that there is a tendency.

(Smoking habit and concentration of zinc in seminal plasma and 8-OHdG concentration in seminal plasma)
The relationship between smoking habit and seminal plasma zinc concentration is shown in FIG. 15, and the relationship between smoking habit and seminal plasma 8-OHdG concentration is shown in FIG. As shown in FIG. 15 and FIG. 16, with or without smoking habit (with: occasional sucks, sucks several days a day, sucks more than one box a day, none: does not suck at all), tendency of zinc concentration in seminal plasma and It was found that the concentration of 8-OHdG in seminal plasma is different, and the smoking habit tends to lower the concentration of zinc in seminal plasma and the concentration of 8-OHdG in seminal plasma.

From the above results, it has been found that the zinc concentration in seminal plasma is a good indicator of the total sperm count and, for example, a good indicator of fertility (fertility level) represented by the WHO standard. Also, it has been found that by appropriately determining the standard of fertility level such as, for example, the total sperm count, the seminal plasma zinc concentration against the standard can be used as a standard value of fertility level. In addition, since the concentration of zinc in seminal plasma also changes depending on the content of the diet, it is understood that lifestyle advice for improving the level of fertility can be made in order to increase the concentration of zinc in seminal plasma, in other words The

Claims (17)

A method of evaluating health characteristics,
Measuring at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in seminal plasma;
Evaluating the health characteristics of the donor of the seminal plasma based on the seminal plasma concentration of the at least one component;
A method comprising.   The method according to claim 1, wherein at least the testosterone concentration and the oxidative stress marker concentration are measured.   The method according to claim 1 or 2, wherein the oxidative stress marker is 8-hydroxy-2'-deoxyguanosine (8-OHdG).   4. A method according to any of the preceding claims, wherein the health property is assessed by comparing the seminal plasma concentration of the at least one component with a reference value previously determined for the seminal plasma concentration of the at least one component. The method described in.   The method according to claim 4, wherein the threshold is set in association with at least one of the health characteristics selected from the group consisting of aging level, fertility level, oxidative stress level and masculinity level.   Claim: The health characteristic is assessed by comparing the seminal plasma concentration of the at least one component with a predetermined target value for the at least one seminal plasma concentration for at least one component. The method in any one of 1-5.   The method according to claim 6, wherein the target value is set in association with a health characteristic selected from the group consisting of aging level, fertility level, oxidative stress level and masculinity level.   The method according to any one of claims 1 to 7, wherein the health characteristic is the level of fertility of the donor.   9. The method according to any of the preceding claims, wherein the health characteristic is the donor's aging level.   10. The method according to any of the preceding claims, wherein the health property is the oxidative stress level of the donor. Health support method,
Measuring at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in seminal plasma;
Evaluating the health characteristics of the donor of the seminal plasma based on the seminal plasma concentration of the at least one component;
Acquiring health support information based on the evaluation result of the health characteristic;
A method comprising.   The health support information is information according to a health purpose related to at least one health characteristic selected from the group consisting of fertility level, aging level, oxidative stress level and masculinity level of the provider. 11. The method according to 11.   The method according to claim 11, wherein the health support information is lifestyle related information related to a meal.   The method according to any one of claims 11 to 13, wherein the health support information is lifestyle related information related to sleep. An evaluation system for health characteristics,
Seminal plasma separation means for separating seminal plasma from semen;
Measuring means for measuring at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in the seminal plasma;
Means for evaluating the health characteristics of the donor of the semen based on the seminal plasma concentration of the at least one component;
A system comprising: A health support system,
Seminal plasma separation means for separating seminal plasma from semen;
Measuring means for measuring at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in seminal plasma,
An evaluation means for evaluating a health characteristic of a donor of the seminal plasma based on the seminal plasma concentration of the at least one component;
Acquisition means for acquiring health support information based on the evaluation result of the health characteristic;
A system comprising: A health support system,
Measuring means for measuring at least one component selected from the group consisting of testosterone, zinc, an oxidative stress marker and creatine in seminal plasma,
Means for inputting concentration information on seminal plasma concentration of said at least one component;
An evaluation means for evaluating a health characteristic of a donor of the seminal plasma based on the input concentration information;
Acquisition means for acquiring health support information based on the evaluation result of the health characteristic;
A system comprising: