JP2019055293A - Biological information imaging apparatus, biological information analysis method, and biological information imaging method - Google Patents

Abstract

To provide a new biological information imaging apparatus, a biological information imaging method, and a biological information analysis method.SOLUTION: The biological information imaging apparatus comprises: a light source 11; an acoustic wave detection device 13 which detects acoustic waves generated from a light absorber 19 in a living body having absorbed a portion of the energy of light applied from the light source 11 to the living body and converts the result to a first electric signal; a light detection device 14 which detects the intensity of the light applied from the light source 11 to the living body and transmitted through the living body, and converts the result to a second electric signal; and an arithmetic unit 22 which utilizes a result of analysis of one of the first electric signal or the second electric signal for analysis of the other, thereby calculating optical characteristic value distribution information of the living body.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a biological information imaging apparatus and a biological information analysis method. Furthermore, the present invention relates to a biological information imaging method.

  Research on an optical imaging apparatus that obtains in-vivo information by propagating light irradiated on a living body from a light source such as a laser into the living body and detecting the propagating light is actively promoted in the medical field.

  As one of the optical imaging, there is a technique called DOT (Diffuse Optical Tomography) called diffuse optical imaging as described in Non-Patent Document 1. Diffuse light imaging is a technology that irradiates a living body with light from a light source, detects weak light that has propagated and diffused inside the living body with a highly sensitive photodetector, and images the distribution of optical characteristic values in the living body from the detection signal It is.

Light irradiated from a light source that has passed through a relatively thick tissue loses wave properties such as straightness of light as a result of being strongly scattered, and diffusely propagates through living tissue. Therefore, the optical characteristic values (absorption coefficient μ _a , equivalent scattering coefficient μ _s ′) of the living tissue can be obtained by optically measuring the intensity of such diffused light at multiple points and processing it with a computer. Furthermore, by measuring these optical characteristic values at different wavelengths, it is possible to obtain the concentration distribution of substances constituting the living tissue.

  On the other hand, as one of optical imaging other than DOT, there is a technology called PAT (Photo Acoustic Tomography) called photoacoustic imaging. Photoacoustic imaging is a technique for obtaining an optical characteristic value distribution in a living body with high resolution by utilizing the characteristics of ultrasonic waves that are less scattered in the living body than light.

  In this method, pulsed light generated from a light source is irradiated on a living body, and an acoustic wave generated from a living tissue that has absorbed energy of pulsed light that has propagated and diffused in the living body is detected. By analyzing this detection signal, an optical characteristic distribution in the living body, in particular, a light energy absorption density distribution can be obtained. By using this photoacoustic imaging, it is said that an optical characteristic value distribution with high resolution can be obtained as compared with the diffused light imaging described above.

  According to Non-Patent Document 2, in photoacoustic imaging, the sound pressure P of an acoustic wave obtained from an absorber in a living body by light absorption is given by the following equation (1).

P = Γ · μ _a · Φ (1)
Here, Γ is a Grunesen coefficient, which is an elastic characteristic value, and is obtained by dividing the product of the square of the volume expansion coefficient β and the speed of sound c by the specific heat Cp. μ _a is the absorption coefficient of the absorber, and Φ is the amount of light irradiated to the absorber.

Since Γ is known to take a substantially constant value when the tissue is determined, the product of μ _a and Φ, that is, the light, is measured by measuring the change in the sound pressure P, which is the magnitude of the acoustic wave, in a time-sharing manner. An energy absorption density distribution H can be obtained (Non-Patent Document 2).

A. P. Gibson, et al. Regent advancements in diffusion optical imaging, Phys. Med. Biol. 50 (2005) R1-R43 M, Xu, L.M. V. Wang ¨ Photoacoustic imaging in biomedicine ¨ Review of scientific instruments, 77, 041101 (2006)

  An object of the present invention is to provide a novel biological information imaging apparatus, a biological information imaging method, and a biological information analysis method.

  The biological information imaging apparatus according to the present invention detects an acoustic wave generated from a light source and an in-vivo light absorber that has absorbed a part of the energy of light irradiated on the living body from the light source, and the first electrical signal An acoustic wave detector for converting to light, a light detector for detecting light intensity of light propagating in the living body in light irradiated on the living body from the light source, and converting the detected light intensity into a second electric signal, and the first electric A calculation unit that calculates optical characteristic value distribution information of the living body by using an analysis result of one of the signal and the second electric signal for analysis of the other electric signal, To do.

  In addition, the biological information analysis method according to the present invention detects an acoustic wave generated from a light absorber in a living body that has absorbed a part of the energy of light irradiated on the living body from a light source, and converts it into a first electrical signal. An electrical signal obtained from an acoustic wave detector for conversion and a photodetector for detecting the light intensity of light propagating in the living body in the light irradiated on the living body from the light source and converting it to a second electrical signal. An analysis method of biological information to be analyzed, wherein an analysis result of one of the first electric signal and the second electric signal is used for analysis of the other electric signal, whereby the optical characteristics of the living body The method has a step of calculating a value distribution.

  Further, another biological information imaging apparatus according to the present invention detects an acoustic wave generated from a light source and an in-vivo light absorber that has absorbed a part of the energy of light irradiated to the living body from the light source, An acoustic wave detector for converting into one electrical signal, a photodetector for detecting the light intensity of light propagating in the living body in the light irradiated on the living body from the light source, and converting it into a second electrical signal; A first information processing unit that analyzes the first electrical signal and obtains optical characteristic value distribution information of the living body; the optical characteristic value distribution information obtained by the first information processing unit; And a second information processing unit that analyzes signals and obtains optical characteristic value distribution information of the living body.

  According to the present invention, a novel biological information imaging apparatus, a biological information imaging method, and a biological information analysis method can be provided.

It is a figure explaining the structural example of the biological information imaging device in Embodiment 1 of this invention. It is a figure explaining the structural example of the biological information imaging device in Embodiment 2 of this invention. It is a figure explaining the structural example of the biological information imaging device in Embodiment 3 of this invention. The flowchart demonstrated in Embodiment 4 of this invention is shown. 7 is a flowchart described in the fifth embodiment of the present invention. It is a figure explaining the imaging technique using photoacoustic imaging and diffused light imaging.

  The imaging apparatus, imaging method, and analysis method according to the present invention are characterized in that data obtained by diffuse light imaging (DOT) and photoacoustic imaging (PAT) are mutually used.

  Both diffused light imaging and photoacoustic imaging are methods for obtaining an absorption coefficient value distribution of an absorber present in a living body. Therefore, if the diffused light imaging or photoacoustic imaging is combined with another imaging method, an imaging method that can obtain information other than the absorption coefficient value distribution such as MRI or X-ray is usually selected. On the other hand, the present invention can be said to be a novel imaging method made under a novel idea in that the methods for obtaining the absorption coefficient value distribution are combined.

  Specifically, ultrasonic waves are measured by photoacoustic imaging, and diffused light is measured by diffused light imaging. And the analysis result obtained by one imaging is utilized for the analysis in the other imaging. The present inventors have found that there are various effects as described below by fusing diffused light imaging and photoacoustic imaging in this way.

(DOT: diffused light imaging)
According to the diffused light imaging described above, it is possible to measure the optical characteristics such as the absorption coefficient and obtain the concentration distribution of the substance constituting the living tissue.

  However, since this diffused light imaging detects light, multiple scattering of light occurs in the medium, and it is difficult to obtain a high-resolution image.

  In general, in light diffusion imaging, imaging is performed by comparing detection results from a plurality of detectors that detect diffuse light with outputs from each detector based on calculation, that is, solving an inappropriate inverse problem. Therefore, generally a long calculation time is required.

  Therefore, when calculating the inappropriate inverse problem, optical characteristic distribution information (for example, absorption coefficient distribution) of the object obtained in advance by photoacoustic imaging is used. As a result, a constraint condition can be added to the diffusion equation to limit the solution, and the calculation time can be shortened.

  Further, by limiting the calculation target region based on information obtained in advance by photoacoustic imaging, it is possible to reduce the calculation amount of the diffusion equation and shorten the calculation time.

  Furthermore, if information obtained by photoacoustic imaging is used in the calculation of the inverse problem of light, an image with higher resolution than that of normal light diffusion imaging can be obtained.

(PAT: Photoacoustic imaging)
According to the photoacoustic imaging described above, since the scattering of acoustic waves in the living body is less than that of light, it is possible to obtain spatial biological information of several mm or less with high resolution.

As can be seen from the above equation (1), in this photoacoustic imaging, in order to obtain the absorption coefficient (μ _a ) distribution in the living body from the measurement of the sound pressure (P) change, the amount of light applied to the absorber is measured. It is necessary to obtain the distribution (Φ) by some method.

However, in a complicated living body, it is difficult to estimate the amount of light irradiated to the absorber, and only the light energy absorption density distribution (μ _a · Φ) can be imaged only by measuring the sound pressure of a general acoustic wave. There is a problem that you can not.

That is, it is difficult to calculate the distribution (Φ) of the amount of light irradiated to the absorber from the measurement of only the acoustic wave, and to accurately separate and image the absorption coefficient distribution (μ _a ) in the living body.

As a result, the photoacoustic imaging alone cannot obtain an accurate distribution of the absorption coefficient (μ _a ), and there is a problem that it is impossible to specify a constituent material of a living tissue and to measure a concentration.

  Therefore, the data obtained by diffused light imaging can be used as an absorption coefficient used in photoacoustic imaging. Specifically, the light quantity (Φ) can be determined independently of ultrasonic measurement from the following diffusion equation approximation (one of light propagation model equations).

Here, ▽ the derivative with respect to space, .mu.s' the equivalent scattering coefficient, mu _a is the absorption coefficient, [nu light propagation velocity in the living body, S 0 _(r, t) is the source of the tissue.

That is, according to diffused light imaging, the distribution of the light quantity (Φ) that cannot be directly measured by photoacoustic imaging can be directly determined. Therefore, if the light quantity (Φ) obtained by diffuse light imaging and the light energy absorption density distribution (μ _a · Φ) obtained by photoacoustic imaging are used, the absorption coefficient distribution can be obtained quantitatively and with high resolution. it can.

  As described above, by combining photoacoustic imaging and diffused light imaging, the quantitativeness and resolution of the absorption coefficient distribution can be improved. Thereby, it is also possible to obtain the concentration of the substance constituting the living body. In addition, it is possible to obtain an accurate value of the Gruneisen coefficient (Γ) distribution, which is treated as a constant in normal acoustic wave imaging.

The above principle will be described with reference to FIGS. FIG. 6A shows an example of a subject 62 that performs internal imaging with an imaging technique using light, and a light absorber 63 exists inside the subject. Reference numeral 61 denotes light emitted from the light source to the subject, and the light absorber inside is imaged using this light. Reference numeral 64 denotes the absorption coefficient value distribution of the dotted line portion in FIG. FIG. 6B shows an example in which the subject in FIG. 6A is imaged using a conventional photoacoustic imaging technique. Reference numeral 66 denotes an imaging image, and reference numeral 65 denotes a light energy absorption density distribution (μ _a · Φ) in a dotted line portion in the figure. As can be seen from this figure, the optical energy absorption density distribution (μ _a · Φ) image that can be imaged by conventional photoacoustic imaging is greatly different from the actual absorption coefficient distribution (μ _a ) image. In other words, in a living body or the like, the local light quantity is greatly attenuated in proportion to the propagation distance due to light diffusion, so that the absorption coefficient and size of the light absorber far from the light irradiation region and the light absorber close to it are Even in the same case, a distant light absorber is imaged as low contrast.

  On the other hand, if the fusion technique of photoacoustic imaging and diffused light imaging of the present invention is used, an image as shown in FIG. 6C can be obtained. In FIG. 6C, 68 is an imaging image, and 67 shows the absorption coefficient value distribution of the dotted line portion in the figure. As described above, in the present invention, even if the light absorber exists far from or near the light irradiation region, if the size and the absorption coefficient are the same, images can be formed with substantially the same contrast. That is, an image proportional to the actual absorption coefficient distribution can be obtained.

(Image reconstruction algorithm)
At the time of imaging, for example, the detection result obtained by each light detector obtained by a diffused light imaging apparatus, and the result of calculating the output result at each detector from the light diffusion equation by estimating parameters as described above Compare If both results match, the result is imaged.

  On the other hand, if the two results do not match, the equivalent scattering coefficient distribution and the absorption coefficient distribution are assumed, the detection results at each detector are calculated again from the diffusion equation, and compared again with the measurement results by PAT.

  It is also possible to perform imaging by repeating the above operation. In the comparison process, in addition to the case where both numerical values completely match, an allowable error range may be determined in advance, and within the error range, it may be determined that both match. Is possible.

  Further, when reconstructing the image of the object, the following algorithm may be used.

1) The size (d) and position (x, y, z) of the absorber are determined by photoacoustic imaging. 2) Using diffused light imaging, the information on the position and size acquired in (1) above is used to reverse the light intensity (Φ), absorption coefficient (μ _a ), and equivalent scattering coefficient (μ _s ′) at each location. Calculate by problem calculation.

  3) The absorption coefficient (μa) is calculated for the photoacoustic imaging result by using the light quantity (Φ).

  Next, embodiments of the present invention will be described with reference to the drawings.

[Embodiment 1]
First, the biological information imaging apparatus according to Embodiment 1 of the present invention will be described. FIG. 1 illustrates a configuration example of a biological information imaging apparatus according to the present embodiment. The biological information imaging apparatus according to the present embodiment is used for diagnosis of tumors such as breast cancer and vascular diseases, follow-up of chemical treatment, etc. This makes it possible to image the concentration distribution of substances constituting the tissue.

  The biological information imaging apparatus of the present embodiment includes a light source 11 that irradiates light to the living body 18 and an optical waveguide 12 that guides the light irradiated from the light source 11 to the living body 18.

  In addition, an acoustic wave detection in which an acoustic wave 21 generated by absorbing a part of light energy by a light absorber 19 in a living body similar to a tumor, blood vessel, or the like in the living body is detected and converted into a first electric signal. A container 13 is provided.

  Moreover, the optical detector 14 which detects the intensity | strength of the light 20 after the said light diffuses in the living body, and converts it into a 2nd electrical signal is provided.

Moreover, the calculation part 22 which acquires 1st optical characteristic value distribution information by the analysis of a 1st electric signal is provided. As the first optical characteristic value distribution information, the size of the absorber, the position where the absorber exists, the value related to the size of the absorption coefficient (for example, the distribution of the product of absorption coefficient and light quantity (μ _a · Φ) Light energy absorption density distribution).

In addition, the calculation unit 22 can analyze the second electrical signal using the first optical characteristic value distribution information and obtain the second optical characteristic value distribution information or the elastic characteristic value distribution. The second optical characteristic value distribution information includes the amount of light irradiated to the absorber (Φ), the absorption coefficient (μ _a ) of the absorber, and the equivalent scattering coefficient (μ _s ′). In addition, the elastic characteristic value distribution is a distribution of a Grueneisen coefficient (Γ).

  Thus, the calculation time can be shortened by using the information obtained by the photoacoustic imaging in the calculation of the inappropriate inverse problem in the light diffusion equation.

  By the way, although diffused light imaging is inferior in terms of resolution compared to photoacoustic imaging, it is advantageous in terms of quantitativeness. Therefore, if the low-quantitative absorption coefficient distribution obtained by the first signal processing unit 15 is used in the light inverse problem calculation, the resolution is determined by photoacoustic imaging, and therefore the resolution is higher than that of diffused light imaging. Imaging can be realized.

  The biological information imaging apparatus according to the present embodiment includes the following units.

  The light source 11 is used as means for irradiating light of a specific wavelength that is absorbed by a characteristic component among the components constituting the living body.

  The light source 11 can be composed of one or a plurality of light sources, at least one of which is a light source that generates pulsed light.

  That is, at least one pulsed light source capable of generating pulsed light on the order of several nanometers to several hundred nanoseconds is provided. At that time, the wavelength of the pulsed light is preferably in the range of 400 nm or more and 1600 nm or less.

  A laser is preferable as the light source, but a light emitting diode or the like may be used instead of the laser.

  As the laser, various lasers such as a solid laser, a gas laser, a dye laser, and a semiconductor laser can be used.

  In the present embodiment, a plurality of light sources may be used as described above. In that case, a plurality of light sources that oscillate at the same wavelength may be used in order to increase the irradiation intensity of the light applied to the living body.

  In order to measure the difference in the optical characteristic value distribution depending on the wavelength, a plurality of light sources having different oscillation wavelengths may be used.

  In the case where the number of light sources 11 is one, a difference in optical characteristic value distribution depending on the wavelength can be measured if a dye capable of oscillating wavelength or OPO (Optical Parametric Oscillators) can be used. Regarding the wavelength of the light source to be used, a region of 700 nm to 1100 nm, which is less absorbed in the living body, is preferable.

  When obtaining the optical characteristic value distribution of the living tissue relatively near the surface of the living body, it is also possible to use a wavelength range wider than the above wavelength range, for example, a wavelength range of 400 nm to 1600 nm.

  The optical waveguide 12 in FIG. 1 serves to guide the light emitted from the light source to the living body 18 to be inspected. The optical waveguide 12 is preferably an optical fiber, but if the light source 11 can be disposed in the vicinity of the living body to be inspected, there is no problem even if it is not used.

  When using an optical fiber, it is possible to guide light to the surface of a living body by using a plurality of optical fibers for each light source, or to guide light from a plurality of light sources to a single optical fiber. All light may be guided to the living body using only one optical fiber.

  The acoustic wave detector 13 of the present embodiment detects an acoustic wave generated from a light absorber in the living body that has absorbed a part of the energy of light irradiated to the living body from the light source, and converts it into a first electrical signal. . That is, the acoustic wave detector 13 receives an acoustic wave and outputs a first electrical signal according to the pressure of the received acoustic wave. As the acoustic wave detector 13, a transducer using a piezoelectric phenomenon, a transducer using optical resonance, a transducer using a change in capacitance, or the like can be used.

  In the present embodiment, a case where a plurality of acoustic wave detectors 13 are arranged on the surface of the living body is shown. However, the present invention is not limited to such an arrangement, and the acoustic waves can be detected at a plurality of locations. Just do it.

  That is, since the same effect can be obtained if acoustic waves can be detected at a plurality of locations, one acoustic wave detector 13 may be scanned two-dimensionally on the surface of the living body. Further, an area-type acoustic wave detector 13 may be provided, and the light source 11 and the acoustic wave detector 13 may be disposed to face the living body.

  Further, when the electrical signal obtained from the acoustic wave detector 13 is small, it is preferable to amplify the signal intensity using an amplifier. Further, it is desirable to use an acoustic impedance matching agent for suppressing reflection of sound waves between the acoustic wave detector 13 and the living body 18 that is a measurement target.

  The photodetector 14 of this embodiment detects the light intensity of the light 20 propagating in the living body in the light irradiated from the light source 11 to the living body, and converts it into a second electrical signal. That is, the photodetector 14 receives the propagation light and outputs a second electrical signal according to the intensity of the received propagation light.

  As the photodetector 14, a photodiode (PD), an avalanche photodiode (APD), a photomultiplier tube (PMT), or the like can be used.

  In addition, when the photodetector 14 cannot be arrange | positioned in the vicinity of the biological body 18 which is a test object, it is also possible to guide light to the photodetector using an optical fiber.

  In this embodiment, an example in which a plurality of photodetectors 14 are used is shown. However, the present invention is not limited to such a configuration, and one photodetector 14 is attached to the surface of the living body in the same manner as the acoustic wave detector 13. You may scan two-dimensionally above. Further, an area-type photodetector 14 may be provided, and the light source 11 and the photodetector 14 may be arranged to face each other.

  The calculation unit 22 can store the intensity of acoustic waves and changes with time, and can convert the information into data of optical characteristic value distribution. Further, the light intensity can be stored and converted into data of optical characteristic value distribution by a calculation means. Then, calculation processing is performed in which the data of the optical characteristic value distribution obtained by one of acoustic wave imaging or diffused light imaging is used for the other imaging. As the calculation unit 22, for example, an oscilloscope and a computer capable of analyzing data stored in the oscilloscope can be used.

  The processing unit that obtains the optical characteristic value distribution information by analyzing the first electrical signal is the first information processing unit, and the processing unit that obtains the optical characteristic value distribution information by analyzing the second electrical signal is the second information. It can also be a processing unit.

  In this case, the information obtained by the first information processing unit can be used for information processing by the second information processing unit, and the information obtained by the second information processing unit can be used by the first information processing unit. It can also be used for information processing. For example, after the first information signal is analyzed by the first information processing unit and the optical characteristic value distribution information of the living body is obtained, the second information processing unit obtains the optical characteristic value distribution information and the second electric value distribution information. You may comprise so that the optical characteristic value distribution information of a biological body may be obtained by analyzing a signal.

When light of a plurality of wavelengths is used as the light source 11, a living tissue is configured by calculating an in vivo absorption coefficient (μ _a ) and an equivalent scattering coefficient (μ _s ′) for each wavelength. The concentration distribution of the substance to be imaged can be imaged. In other words, by comparing these optical property values with the wavelength dependence of the substances that make up living tissue such as glucose, collagen, and oxidized / reduced hemoglobin, the concentration distribution of the substances that make up the living body is imaged. It is also possible to do.

  Furthermore, by analyzing the optical characteristic value distribution value obtained from the first electric signal and the optical characteristic value distribution value obtained from the second electric signal, elasticity in the living body, which was difficult in the prior art, was analyzed. It is also possible to measure the characteristic value distribution, that is, the Gruneisen coefficient distribution.

Specifically, from the amount of light (Φ) irradiated to the absorber obtained by the second electrical signal and the product of the light absorption coefficient and the amount of light obtained by the first electrical signal (μ _a · Φ), The absorption coefficient (μ _a ) of the absorber is obtained. Then, by applying the sound pressure obtained from the first electric signal to the equation (1), the Gruneisen coefficient distribution is calculated. The light quantity (Φ) can be obtained by analyzing the second electric signal using the optical characteristic value distribution value obtained by the first electric signal as described above. It can also be obtained by analyzing only the second electrical signal.

  In the embodiment of the present invention, it is desirable to include the image display unit 17 that displays the image information obtained by the processing of the calculation unit 22.

[Embodiment 2]
Next, a biological information imaging apparatus according to Embodiment 2 of the present invention will be described.

  FIG. 2 illustrates a configuration example of the biological information imaging apparatus according to this embodiment. In addition, the same code | symbol is attached | subjected about the same structure as FIG.

  The light source in this embodiment includes at least two light sources including a first light source 11 that generates pulsed light and a second light source 23 that generates light having a waveform different from that of the pulsed light.

  In addition, the light emitted from the first light source 11 to the living body can be converted into the first electric signal by the acoustic wave detector 13. Further, the light irradiated on the living body from the second light source 23 can be converted into the second electric signal by the photodetector 14.

  In addition, a calculation unit 22 is provided that analyzes the optical characteristic value distribution information obtained by analyzing the first electric signal and the second electric signal to obtain the optical characteristic value distribution of the living body. In addition, this calculating part 22 can also obtain | require the optical characteristic value distribution of a biological body by analyzing the optical characteristic value distribution information obtained by the analysis of a 2nd electric signal, and a 1st electric signal.

  The biological information imaging apparatus of the present embodiment is configured by the above-described units.

  The first light source 11 irradiates pulse light having a specific wavelength that is absorbed by a characteristic component among components constituting the living body.

  The first light source is preferably a laser capable of generating pulsed light on the order of several nanometers to several hundred nanoseconds, and the same light source as that of the first embodiment can be used. In the present embodiment, there is one first light source, but a plurality of light sources may be used.

  Similarly to the first light source, the second light source 23 irradiates light having a specific wavelength that is absorbed by the characteristic component among the components constituting the living body.

  The light generated from the second light source is either continuous light, intensity-modulated light, or pulsed light having a different pulse width from the first pulsed light source.

  When continuous light is used, various coherent light and non-coherent light sources such as a semiconductor laser and a light emitting diode can be used.

  As the light source that oscillates the intensity-modulated light and the pulsed light, various coherent light and non-coherent light source such as a semiconductor laser and a light emitting diode can be used.

  In the present embodiment, there is one first light source, but a plurality of light sources may be used. As for the optical waveguide 12, the acoustic wave detector 13, the photodetector 14, and the calculation unit 22 in FIG. 2, the same ones as in the first embodiment can be used.

  As described above, in this embodiment, the pulse light for photoacoustic imaging and the light for diffused light imaging are separately provided, so that it is possible to obtain the optical characteristic value distribution in various regions with high resolution. .

[Embodiment 3]
Next, a biological information imaging apparatus according to Embodiment 3 of the present invention will be described.

  FIG. 3 illustrates a configuration example of the biological information imaging apparatus according to this embodiment. The biological information imaging apparatus according to the present embodiment is used to diagnose a variety of diseases using a contrast medium such as a malignant tumor diagnosis, Alzheimer's disease, and carotid artery plaque. Imaging is possible.

  At that time, the photodetector for converting into the second electric signal is configured to detect the light intensity of light having a wavelength different from that of the light of the light source when detecting the intensity of the light propagated in the living body. Yes.

  That is, the light intensity of light having a wavelength different from that of the light of the light source generated from the light absorber (contrast agent) in the living body that absorbs a part of the energy of light irradiated to the living body from the light source can be detected. Has been.

  Specifically, the biological information imaging apparatus according to the present embodiment includes a light source 11 for generating first light and an optical waveguide 12 for guiding the first light emitted from the light source to the living body 18.

  Further, the acoustic wave 21 generated by absorbing a part of the first light energy irradiated to the living body 18 through the optical waveguide 12 by the contrast medium 30 introduced into the living body is detected, and the first electric signal is detected. An acoustic wave detector 13 for converting to is provided.

  Further, the second light 31 generated by absorbing a part of the first light energy irradiated to the living body from the optical waveguide is detected and converted into a second electric signal after the propagation of the second light 31 in the living body. A photodetector 14 is provided.

  In addition, a calculation unit 22 is provided that analyzes the optical characteristic value distribution information obtained by analyzing the first electric signal and the second electric signal to obtain the optical characteristic value distribution of the living body. In addition, this calculating part 22 can also obtain | require the optical characteristic value distribution of a biological body by analyzing a 1st electrical signal using the optical characteristic value distribution information obtained by the analysis of a 2nd electrical signal.

  The biological information imaging apparatus of the present embodiment is configured by the above-described units.

  The light source 11 of FIG. 3 irradiates pulse light (first light) having a specific wavelength that is absorbed by the contrast medium 30 introduced into the living body.

  The light source 11 is preferably a laser capable of generating pulsed light on the order of several nanometers to several hundred nanoseconds, and the same light source as that of the first embodiment can be used.

  In the present embodiment, the number of light sources 11 is one, but a plurality of light sources may be used.

  As the optical waveguide 12, the sound wave detector 13, the light detector 14, and the calculation unit 22 in FIG. 2, the same ones as those in the first embodiment can be used.

  The second light 31 is generated from the contrast medium 30, and the first light irradiated from the light source 11 is light having a different wavelength.

  Desirably, the fluorescence emitted from the contrast medium 30 is desirable. As contrast medium 30, typically Indian Indian Green (ICG) or the like is used. However, as long as it emits an acoustic wave when irradiated with pulsed light and emits light having a wavelength different from that of pulsed light, Any substance can be used.

  By using the biological information imaging apparatus shown in such an embodiment, the optical property distribution of the contrast agent introduced into the living body can be obtained more accurately and imaged than in the prior art.

[Embodiment 4]
In the present embodiment, a biological information analysis method using electrical signals obtained from an acoustic wave detector and a photodetector will be described.

Hereinafter, with reference to FIG. 4, the absorption coefficient (μ _a ) and equivalent scattering coefficient (μ _s ′), which are optical characteristic value distributions in the living body, and the Gruyenesen coefficient distribution (Γ), which is an elastic characteristic of the living body. An example of a flowchart for obtaining the above will be described.

  The intensity of the light 20 propagated inside the living body is detected by the photodetector and converted into an electric signal (S100).

  The acoustic wave detector detects the acoustic wave signal generated from the light absorber 19 inside the living body and converts it into an electrical signal (S101).

The electrical signal obtained in the step of S101 is analyzed, and an in vivo absorption coefficient (μ _a ) distribution is assumed (S102). As described above, the sound pressure P of the sound wave generated from the light absorber in the living body can be expressed by the following equation.
P = Γ · μ _a · Φ

Here, Γ is a Gruneisen coefficient, μ _a is an absorption coefficient of the absorber, and Φ is an amount of light irradiated to the light absorber.

Assuming that the Gruneisen coefficient is almost constant in the tissue, the product of the absorption coefficient (μ _a ) and the amount of light (Φ), that is, the distribution of the light energy absorption density (H) is obtained by time-resolved measurement of the sound pressure (P). Can be sought. Here, the quantity of light (Φ) decays exponentially according to the distance from the light source, so qualitative absorption in the living body is assumed by assuming a coefficient in the exponential function such as using the average value of the living body. A distribution of coefficients (μ _a ) can also be assumed (S102).

Further, based on the distribution of the absorption coefficient (μ _a ) obtained in the step of S102, the distribution of the equivalent scattering coefficient (μ _s ′) is also assumed (S103).

Using the absorption coefficient (μ _a ) and equivalent scattering coefficient (μ _s ′) assumed in steps S102 and S103, a light propagation model using a radiation transport equation (Boltzmann equation) or a diffusion equation is solved, and each photodetector is detected. The light intensity that can be measured is calculated (S104).

The light intensity value obtained in step S104 is compared with the light intensity actually measured in step S100 (S105). If they do not match, the process returns to step S102, and a new in vivo absorption coefficient (μ _a ) and equivalent scattering coefficient (μ _s ′) are assumed, and the process is repeated until the difference becomes small. In other words, the comparison in step S105 does not always have to be repeated until a perfect match is obtained, and it may be determined that the comparison is based on whether or not the calculation residual satisfies the convergence condition.

  If the light intensity obtained by calculation in step S105 matches the light intensity obtained by measurement, or if the residual of the calculation satisfies the convergence condition, the assumed absorption coefficient distribution and the equivalent scattering coefficient distribution are converted into the absorption coefficient in the living body. And the equivalent scattering coefficient distribution are determined (S106).

Using the distribution of the absorption coefficient (μ _a ) in the living body and the equivalent scattering coefficient (μ _s ′) determined in step S106, the distribution of the light quantity (Φ) is obtained, and the absorption coefficient (μ _a ) and the light quantity in the living body are obtained. The product of (Φ), that is, the distribution of the light energy absorption density (H) is obtained (S107).

  From the distribution of the sound pressure measured at the step S101 and the distribution of the light energy absorption density obtained at the step S107, an in-vivo Grueneisen coefficient (Γ) distribution is obtained (S108).

  Note that the sound pressure measurement in step S101 may be performed before the light intensity measurement in step S100.

Further, the assumption of the equivalent scattering coefficient (μ _s ′) by the step of S103 may be performed before the assumption of the absorption coefficient (μ _a ) by the step of S102.

  Furthermore, when it is desired to obtain only the absorption coefficient distribution in the living body, it is possible to end the process in step S106.

  By performing this calculation in the calculation unit, the optical characteristic value distribution such as the absorption coefficient and equivalent scattering coefficient of the living body to be measured and the elastic characteristic distribution of the living body such as the Gruyensen coefficient are accurately imaged. It becomes possible to do.

  Moreover, it is also possible to image the concentration distribution of substances (glucose, collagen, oxidized / reduced hemoglobin, etc.) constituting the living tissue using these values.

[Embodiment 5]
Next, an example of a flowchart for obtaining the distribution of the absorption coefficient (μ _a ) and the scattering coefficient (μs ′), which are optical characteristic value distributions in the living body, will be described with reference to FIG. In FIG. 5, the same steps as those in the fourth embodiment are denoted by the same reference numerals.

  The light propagation model is solved by the same processing as in the fourth embodiment, and the light intensity that can be measured by each photodetector is calculated (S104).

Using the distribution of the absorption coefficient (μ _a ) and the equivalent scattering coefficient (μ _s ′) in the living body obtained in step S104, the distribution of the light quantity (Φ) in the living body is obtained (S200).

The distribution of the absorption coefficient (μ _a ) in the living body is determined from the magnitude of the acoustic wave measured in step S101 and the distribution of the light quantity (Φ) obtained in step S200.

  By performing such a calculation by the calculation unit, it is possible to accurately form an absorption coefficient distribution image of a living body to be measured.

  Furthermore, using these values, it is possible to image the concentration distribution of substances (such as glucose, collagen, oxidized / reduced hemoglobin) constituting the living tissue.

  Note that the method described in the fourth and fifth embodiments can be realized by using the apparatus described in the first to third embodiments.

In the above, the light quantity (Φ) is obtained from diffused light imaging using acoustic wave data, but the distribution of the light quantity (Φ) is obtained directly from diffused light imaging, and the absorption coefficient ( It is also possible to determine the distribution of μ _a ).

  Here, the method of obtaining the light absorption coefficient, the equivalent diffusion coefficient, and the Gruneisen coefficient distribution is used based on the above flowcharts, but the present invention is not limited to these flowcharts.

  The essence of the present invention is that the acoustic wave generated by the energy absorption of the light irradiated on the living body from the light source and the light intensity after the light irradiated on the living body propagates through the living body are detected, and each information is mutually exchanged. It is to obtain the optical characteristic value distribution in the living body accurately by using and analyzing.

  Therefore, the present invention is not limited to the flowchart described above.

  Next, an invention relating to a biological information imaging method according to the present invention will be described.

  The essence of the method is to obtain distribution information regarding the optical characteristics of the imaging object in the living body by measuring diffused light of light incident on the living body at a plurality of locations, and using the distribution information, The following measures are taken when imaging information related to the imaging object.

  That is, the imaging is performed using information based on elastic waves output by the imaging object. Here, the distribution information relating to the optical characteristics of the imaging object is an absorption coefficient and / or an equivalent scattering coefficient. Of course, the imaging method of the present invention does not exclude other optical characteristics.

  Hereinafter, the present invention will be described using examples.

  One embodiment of the present invention will be described. An example of a biological information imaging apparatus for the purpose of obtaining the absorption coefficient distribution of blood vessels in the living body will be described with reference to FIG.

  As the light source 11, a Q-switched Nd: YAG laser capable of oscillating 1064 nm nanosecond pulsed light is used. The pulse width is about 5 nanoseconds and the repetition rate is 10 Hz. As another light source 23, a 1060 nm semiconductor laser is used. At this time, the light is modulated. The modulation frequency is 100 MHz. Moreover, since the light source 23 is irradiated from a plurality of positions, the light source 23 is movable. Here, the light source 23 can be irradiated at eight locations.

  As the acoustic wave detector 13, 256 piezo-type transducers having a center frequency of 1 MHz are used. This array is arranged two-dimensionally.

  As the photodetector 14, eight photomultiplier tubes (PMT) are used. In order to put the light propagated in the living body into the photodetector, an optical fiber not shown in FIG. 1 is coupled to the surface of the living body, and the propagating light is guided from there to the photodetector 14.

  The acoustic wave signal and the optical signal converted into the electrical signal are recorded by an oscilloscope, and then sent to a computer for analysis.

  Using such a device, if the measurement is performed with a sample simulating a blood vessel embedded in soft tissue, the absorption coefficient can be accurately measured compared to the conventional method of imaging the absorption coefficient distribution using only sound waves. It becomes possible to reproduce the distribution.

DESCRIPTION OF SYMBOLS 11 Light source 12 Optical waveguide 13 Acoustic wave detector 14 Photo detector 17 Image display part 18 Living body 19 Light absorber 20 Light propagating in the living body 21 Acoustic wave 22 Calculation part 23 Second light source 30 Contrast agent 31 In vivo Second light propagating through light 61 Light 62 Subject 63 Light absorber 64, 67 Light absorption coefficient distribution 65 Light energy absorption density distribution 66, 68 Imaging image

An information processing apparatus according to the present invention includes: a first unit that acquires an image based on acoustic wave information related to an acoustic wave generated due to light irradiated on a subject; A second unit that executes a process for correcting an influence on the image value of the image derived from the variation in the amount of light of the image, and the image value of the image corresponding to the target position of the subject is: It is an image value calculated based on the acoustic wave information obtained by receiving the acoustic wave generated at the target position at a plurality of positions.

Claims (19)

A biological information imaging apparatus comprising:
A light source;
An acoustic wave detector that detects an acoustic wave generated from a light absorber in the living body that has absorbed a part of the energy of light irradiated to the living body from the light source, and converts the detected acoustic wave into a first electrical signal;
A light detector for detecting the light intensity of light propagating in the living body in the light irradiated on the living body from the light source, and converting it into a second electrical signal;
An arithmetic unit that calculates optical characteristic value distribution information of the living body by using an analysis result of one of the first electric signal and the second electric signal for analysis of the other electric signal;
A biological information imaging apparatus comprising:   The calculation unit calculates second optical characteristic value distribution information by using first optical characteristic value distribution information, which is an analysis result of the first electric signal, for analysis of the second electric signal. The biological information imaging apparatus according to claim 1.   The first optical characteristic value distribution information is any one of a size of the light absorber, a position where the light absorber exists, and a light energy absorption density distribution of the light absorber. Item 3. The biological information imaging apparatus according to Item 2.   The second optical characteristic value distribution information is any one of a light amount irradiated to the light absorber, an absorption coefficient of the light absorber, and an equivalent scattering coefficient of the light absorber. 3. The biological information imaging apparatus according to 3.   The calculation unit calculates an absorption coefficient distribution of the light absorber by using the amount of light irradiated to the light absorber, which is an analysis result of the second electric signal, for the analysis of the first electric signal. The biological information imaging apparatus according to claim 1.   The biological information imaging apparatus according to claim 1, wherein the light absorber in the living body is a tumor or a blood vessel in the living body.   The biological information imaging apparatus according to claim 1, wherein the acoustic wave detector is configured to be able to detect acoustic waves at a plurality of locations.   The biological information imaging apparatus according to claim 1, wherein the photodetector is configured to be able to detect light intensity at a plurality of locations.   The biological information imaging apparatus according to claim 1, wherein the light source includes one or a plurality of light sources, and at least one of the light sources generates a pulsed light. The light source includes at least two light sources including a first light source that generates pulsed light and a second light source that generates light having a waveform different from that of the pulsed light,
The acoustic wave detector is configured to be able to convert an acoustic wave generated by light irradiated on the living body from the first light source into the first electric signal,
The biological information imaging apparatus according to claim 9, wherein the photodetector is configured to be able to convert light irradiated on the living body from the second light source into the second electrical signal.   The light detector is configured to detect light having a wavelength different from that of the light of the light source generated from an in-vivo light absorber that has absorbed a part of the energy of light irradiated to the living body from the light source. The biological information imaging apparatus according to claim 1, wherein:   The biological information imaging apparatus according to claim 11, wherein the light absorber in the living body is a contrast agent introduced into the living body.   The biological information imaging apparatus according to claim 1, wherein a wavelength of the light source is in a range of 400 nm to 1600 nm. An acoustic wave detector that detects an acoustic wave generated from a light absorber in the living body that absorbs part of the energy of light irradiated to the living body from the light source, and converts the acoustic wave into a first electrical signal; A method for analyzing biological information, which analyzes an electrical signal obtained from a photodetector that detects the light intensity of light propagating through a living body in irradiated light and converts it into a second electrical signal,
Analyzing the light energy absorption density distribution from the first electrical signal;
Using the light energy absorption density distribution to determine the absorption coefficient distribution or equivalent scattering coefficient distribution of the light absorber by analyzing the second electrical signal;
A method for analyzing biological information, characterized by comprising:   The biological information analysis method according to claim 14, further comprising a step of obtaining an elastic characteristic value distribution using an absorption coefficient distribution of the light absorber. An acoustic wave detector that detects an acoustic wave generated from a light absorber in the living body that absorbs part of the energy of light irradiated to the living body from the light source, and converts the acoustic wave into a first electrical signal; A method for analyzing biological information, which analyzes an electrical signal obtained from a photodetector that detects the light intensity of light propagating through a living body in irradiated light and converts it into a second electrical signal,
A step of calculating an optical characteristic value distribution of the living body by using an analysis result of one of the first electric signal and the second electric signal for analysis of the other electric signal; A method for analyzing biological information. A biological information imaging method comprising:
By measuring the diffused light of the light incident on the living body at a plurality of locations, distribution information relating to the optical characteristics of the imaging object in the living body is obtained, and using the distribution information, information relating to the imaging object is obtained. When imaging,
A method for imaging biological information, wherein the imaging is performed using information based on an acoustic wave output by absorbing a part of light irradiated to the imaging object. A biological information imaging method comprising:
Acquire distribution information on the optical characteristics of the imaging object in the living body by measuring the acoustic wave generated from the light absorber in the living body that absorbs part of the energy of the light incident on the living body at multiple locations. Then, when the information about the imaging object is imaged using the distribution information,
A method for imaging biological information, characterized in that the imaging is performed using information based on diffused light propagated through the imaging object. A biological information imaging apparatus comprising:
A light source;
Receives an acoustic wave generated from a light absorber in the living body that has absorbed a part of the energy of light irradiated to the living body from the light source, and outputs a first electrical signal according to the sound pressure of the received acoustic wave. An acoustic wave detector;
A photodetector that receives the propagation light of the light irradiated to the living body from the light source or another light source, and outputs a second electrical signal according to the intensity of the received propagation light;
An arithmetic unit that calculates optical characteristic value distribution information of the living body by using an analysis result of one of the first electric signal and the second electric signal for analysis of the other electric signal;
A biological information imaging apparatus comprising:

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