JP2019006701A - Hair-growing agent, anti-obesity agent, cosmetic, and food/drink product - Google Patents

Abstract

To provide a hair-growing agent with excellent hair-growing action, an anti-obesity agent with excellent anti-obesity action, a cosmetic and a food/drink product containing at least either one of the agent.SOLUTION: There are provided a hair-growing agent and an anti-obesity agent which contain a lactic acid bacterium fermented product produced by fermenting sake lees extract liquid by lactic acid bacterium. There are provided a cosmetic and a food/drink product for at least either one selected from hair-growing use containing a hair-growing agent and anti-obesity use containing an anti-obesity agent. It is a preferable aspect that the lactic acid bacterium is any one of lactobacillus plantarum 22A-1(FERM AP-21409), lactobacillus plantarum 22A-3(FERM AP-21411), and lactobacillus plantarum 22B-2(FERM AP-21410).SELECTED DRAWING: None

Description

  The present invention relates to a hair restorer, an anti-obesity agent, a cosmetic, and a food and drink.

  Sake, shochu, beer, and the like are known as alcoholic fermented alcoholic beverages, and it has been reported that the sake produced as a by-product during the production of these alcoholic beverages has various effects.

  The effects of sake lees (hereinafter also referred to as “sake lees”) include, for example, diabetes, hypertension, osteoporosis, cerebral infarction, angina pectoris, myocardial infarction, prevention of arteriosclerosis, improvement of allergic constitution, and It is known to have a whitening effect.

  Furthermore, methods using fermented sake lees are known. For example, whitening cosmetics blended with fermented liquor of sake lees, liquefied lees that are a by-product when rice liquefied is used as a charging material. A health functional composition obtained by fermenting lactic acid with lactic acid bacteria has been proposed (see, for example, Patent Documents 1 and 2).

  Thus, sake lees have various effects, but development of a preparation with higher hair-growth action and anti-obesity action is desired.

JP 2011-148737 A JP 2007-099731 A

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, an object of the present invention is to provide a hair-restoring agent excellent in hair-restoring action, an anti-obesity agent excellent in anti-obesity action, a cosmetic containing at least one of them, and a food and drink.

Means for solving the problems are as follows. That is,
<1> A hair restorer comprising a fermented lactic acid bacterium obtained by fermenting a sake lees extract with lactic acid bacteria.
<2> The hair restorer according to <1>, wherein the lactic acid bacterium is Lactobacillus plantarum.
<3> An anti-obesity agent comprising a lactic acid bacteria fermented product obtained by fermenting a sake lees extract with lactic acid bacteria.
<4> The anti-obesity agent according to <3>, wherein the lactic acid bacterium is Lactobacillus plantarum.
<5> selected from the hair-restoring agent comprising the hair-restoring agent according to any one of <1> to <2> and the anti-obesity agent comprising the anti-obesity agent according to any one of <3> to <4>. The cosmetic is characterized by being at least one of the following.
<6> Selected for hair growth including the hair restorer according to any one of <1> to <2> and for anti-obesity containing the anti-obesity agent according to any one of <3> to <4> It is a food or drink characterized by being at least one of the above.

  According to the present invention, it is possible to solve the conventional problems described above and provide a hair-restoring agent excellent in hair-restoring action, an anti-obesity agent excellent in anti-obesity action, a cosmetic containing at least one of these, and a food and drink. Can do.

(Hair restorer and anti-obesity agent)
The hair restorer of the present invention contains a fermented lactic acid bacterium obtained by fermenting a sake lees extract with lactic acid bacteria, and further contains other components as necessary.
The anti-obesity agent of the present invention contains a fermented lactic acid bacterium obtained by fermenting a sake lees extract with lactic acid bacteria, and further contains other components as necessary.

  Here, the hair-restoring agent and the anti-obesity agent are those that are less likely to harm human health and are taken orally or by digestive tract administration in normal social life. It is not limited to categories such as pharmaceuticals and quasi drugs, but includes, for example, a wide variety of functional foods, quasi drugs and pharmaceuticals that are taken orally.

<Fermented lactic acid bacteria>
The fermented lactic acid bacterium is a fermented lactic acid bacterium obtained by fermenting the liquor extract with lactic acid bacteria (hereinafter referred to as “fermented lactic acid bacterium of liquor extract”), and further contains other components as necessary. The lactic acid bacteria fermented product of the liquor extract can be used alone as a hair restorer and an anti-obesity agent, and can further contain other components as necessary.

<< Lactic acid bacteria >>
There is no restriction | limiting in particular as said lactic acid bacteria, Bacteria etc. which can be suitably selected according to the objective and can produce lactic acid by fermentation are mentioned. Examples of the lactic acid bacteria include, for example, Lactobacillus, Pediococcus, Bifidobacterium, Lactococcus, Leuconostoc, and Enterococcus cc. ) And the like.

  Examples of the lactic acid bacteria include, for example, Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus salivalus (Lactobacillus salivalus), Lactobacillus cactobacillus Lactobacillus Lactobacillus, (Lactobacillus kitatatonisi), Lactobacillus reuteri (Lactobacillus reuteri), Lactobacillus jhonsonii, Lactobacillus acidophilus (Lactobacillus acidophilus) lus), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus Korinihorumisu (Lactobacillus coryniformis), Lactobacillus Karubatasu (Lactobacillus curvatus), Lactobacillus Deruburyukyi (Lactobacillus delbrueckii), Lactobacillus Farsi Minas (Lactobacillus farciminus), Lactobacillus rhamnosus (Lactobacillus rhamnosus ); Pediococcus damnosus, Pediococcus halofilus, Pedeco as genus Pediococcus Occocus parvallus (Pediococcus parvulus), Pediococcus pentosaceus; Bifidobacterium genus, Bifidobacterium adolecentis, Lactococcus cremoris, Lactococcus lactis; as the genus Leuconostoc, Leuconostoc oenos, Leuconostoc lactis (Leuconos) oc lactis), Leuconostoc mesenteroides (Leuconostoc mesenteroides); as Enterococcus, and the like Enterococcus faecalis (Enterococcus faecalis). Among these, Lactobacillus plantarum (Lactobacillus plantarum) is preferable because it proliferates efficiently and the obtained fermented product has a strong activity.

  There is no restriction | limiting in particular as said Lactobacillus plantarum, Although it can select suitably according to the objective, Lactobacillus plantarum 22A-1 (Accession number: FERM AP-21409), Lactobacillus plantarum 22A-3 ( Accession number: FERM AP-21411) or Lactobacillus plantarum 22B-2 (accession number: FERM AP-21410) is particularly preferred.

-22A-1 strain, 22A-3 strain, and 22B-2 strain-
Lactobacillus plantarum 22A-1 (Accession number: FERM AP-21409), Lactobacillus plantarum 22A-3 (Accession number: FERM AP-21411), Lactobacillus plantarum 22B-2 (Accession number: FERM AP) -21410) is a strain isolated and identified from pickles by the present inventors, and deposited with the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology.

  Lactobacillus plantarum 22A-1 (Accession number: FERM AP-21409), Lactobacillus plantarum 22A-3 (Accession number: FERM AP-21411), Lactobacillus plantarum 22B-2 (Accession number: FERM AP) The mycological properties of three strains of -21410) are shown in Tables 1 and 2 below. In Table 2, the sugar degradability of known Lactobacillus plantarum ATCC 14917 is also shown as a comparison target of the above three strains.

（＋：陽性、−：陰性）

(+: Positive,-: negative)

（＋：陽性、−：陰性）

(+: Positive,-: negative)

<< Liquor extract >>
The sake lees extract is an extract obtained by extracting sake lees with a solvent.

  There is no restriction | limiting in particular as said solvent, According to the objective, it can select suitably, For example, water, a water-soluble organic solvent, etc. are mentioned. Among these, water is preferable from the viewpoint of not inhibiting the activity of lactic acid bacteria.

-Sake lees-
The sake lees are solids that remain after squeezing the moromi used in the production of sake.
There is no restriction | limiting in particular as said liquor, According to the objective, it can select suitably, For example, sake, shochu, awamori, etc. are mentioned.

  A commercial item can be used as said sake lees, for example, white peony liquor (product made from white peony brewing), etc. are mentioned.

[Method of preparing sake lees]
The method for preparing the sake lees is not particularly limited, and conventional methods for preparing sake lees can be used. For example, brown rice is made into white rice and then steamed to make steamed rice. There is a method of adding sake lees, putting sake yeast therein, producing moromi and then squeezing the moromi to obtain sake lees that are solids.

[Method for preparing sake lees extract]
There is no restriction | limiting in particular as a preparation method of the said sake lees extract, According to the objective, it can select suitably, For example, the method of suspending sake lees in a solvent etc. are mentioned.

[Method for preparing fermented lactic acid bacteria]
Examples of the method for preparing the fermented lactic acid bacteria include the following methods. 5 g of sake lees prepared per 100 g of water was added and mixed with stirring to obtain a liquor extract, and then a high-pressure steam sterilizer at 110 ° C. for 5 minutes (device name: MLS-3571-PJ, manufactured by Panasonic) Autoclave with, sterilize and cool. After cooling to about room temperature, 0.1 g of lactic acid bacteria (species name: Lactobacillus plantarum 22A-3, in-house culture) is added, and shaking culture is performed at 30 ° C. for 16 to 24 hours. After culturing, the mixture is sterilized by autoclaving at 110 ° C. for 5 minutes and filtered through diatomaceous earth to obtain a lactic acid bacteria fermentation product.

  There is no restriction | limiting in particular as content of the said lactic-acid-bacteria fermented material, According to the objective, it can select suitably, For example, 0.05 mL / mL or more and 1.0 mL / mL with respect to a hair restorer and an antiobesity agent The following is preferable, and 0.2 mL / mL or more and 0.8 mL / mL or less is more preferable.

  There is no restriction | limiting in particular as a utilization form of the said lactic-acid-bacteria fermented material, According to the objective, it can select suitably, For example, the powder material which lyophilized the filtrate which filtered the lactic-acid-bacteria fermented material with the filter, etc. Is mentioned.

<< Other ingredients >>
The other components are not particularly limited and may be appropriately selected depending on the usage form of the hair restorer and anti-obesity agent. Examples thereof include plant extracts and pharmacologically acceptable carriers. .

-Plant extract-
The plant extract is an extract from a plant.

  Examples of the plant include a thistle, an acha, an opium, an aloe bear, a ginkgo, a fennel, a turmeric, a common beetle, a larva, an age, an elephant, a life-prolonging grass, a yellow spirit, an oat, an ogon, an oak, a barley, a hypericum grass, a moth, a chamomile, and a licorice. , Kidachi Aloe, Gymnema, Cabbage, Jade Bamboo, Kiraya, Gold and Silver Flower, Chrysanthemum, Chrysanthemum, Red Ginseng, Ginseng, Kumagusu, Mulberry, Laurel Leaves, Akiko, Gentiana, Wheat, Rice, Burdock, Sesame, Salvia, Sanza, Shiso, Sanshishi, Sanche, Yam, Yamayaku, Shiitake mushrooms, Purple candy, Glaze, Car forage, Ten ginseng, White ginger, Horsetail, Stevia, Cyprus, Senna, Assembly, Buckwheat, Radish, Taiso, Soy, Tamarind, Cod, Chimpi, Green tea , Tenninka, Tokachi, Tochu, Cordyceps, Corn, Sashimi, Ginseng, Shinobyu, Parsley, Beach windbreak, Mamellisu, himematsu mushroom, bilberry, loquat, bukuro, grape, blueberry, loofah, hematin, linden tree, peony, hops, pine needles, peach, melissa, yucca, yokoinin, mugwort, rye, lakanka, green tea, apple, rooibos, lucas, Examples include ganoderma, reindeer grass, rose hips, rosemary, and bitumen. Among these, green tea is preferable because it contains abundant gallate catechins.

  The plant extract can be easily obtained by a method generally used for plant extraction. In addition, the extract from the plant includes a plant extract, a dried product obtained by drying a diluted solution of the extract, or a crude product or a purified product thereof.

  The plant extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, a plant leaf part, a stem part, a flower (bud) part, a seed (these are referred to as above-ground parts), A root or the like can be used.

  The plant as the raw material for extraction can be obtained by drying or pulverizing it as it is or using a crusher and subjecting it to solvent extraction. Drying may be performed in the sun or using a commonly used dryer. The plant may be used as a raw material for extraction after pretreatment such as degreasing with a nonpolar solvent such as hexane or benzene. In addition, the extraction process by the polar solvent of a plant can be performed efficiently by performing pretreatments, such as degreasing.

As the solvent used for the extraction, it is preferable to use water, a hydrophilic organic solvent, or a mixed solvent thereof at a temperature from room temperature to the boiling point of the solvent.
Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. The water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol, propylene glycol, Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycerin, and a mixed solvent of these hydrophilic organic solvents and water can be used.
In addition, when using the mixed solvent of the said water and the said hydrophilic organic solvent, in the case of a lower alcohol, 1 mass part-90 mass parts with respect to 10 mass parts of water, and in the case of a lower aliphatic ketone, water 10 It is preferable to add 1 part by mass to 40 parts by mass with respect to part by mass. In the case of polyhydric alcohol, it is preferable to add 1 part by mass to 90 parts by mass with respect to 10 parts by mass of water.

  In the present invention, when extracting an extract from a plant as an extraction raw material, it is not necessary to adopt a special extraction method, and the extraction can be performed using an arbitrary extraction device at room temperature or under reflux heating.

  Specifically, a plant as an extraction raw material is put into a treatment tank filled with an extraction solvent, and further, if necessary, after standing for 30 minutes to 2 hours with occasional stirring to elute soluble components. The solid matter is removed by filtration, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The amount of the extraction solvent is usually 5 to 15 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C. when water is used as the extraction solvent. Moreover, when the mixed solvent of water and ethanol is used as an extraction solvent, it is normally 30 minutes-about 4 hours at 40 to 80 degreeC. In addition, the extract obtained by extracting with a solvent can be used as it is as a plant extract as long as the extraction solvent is highly safe.

  The obtained plant extract is diluted, concentrated, dried, or purified according to a conventional method to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crudely purified product or purified product thereof. Processing may be performed.

  Since the plant extract contains an extract from the plant, it can exhibit various physiological functions of the extract from the plant.

  The carrier is not particularly limited as long as the effects of the present invention are not impaired. For example, when the active ingredient is used in various dosage forms, it can be appropriately selected depending on the dosage form. The dosage form is not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected depending on the administration method. For example, oral solids (tablets, coated tablets, granules, powders, capsules) , Lozenges, etc.), oral solutions (internal solutions, syrups, elixirs, etc.).

As the oral solid preparation, for example, additives such as excipients, binders, disintegrants, lubricants, colorants, flavoring / flavoring agents, and the like can be added according to a conventional method.
Examples of the excipient include lactose, sucrose, sodium chloride, glucose, starch, calcium carbonate, kaolin, microcrystalline cellulose, and silicic acid.

Examples of the additive include a binder, a disintegrant, a lubricant, a colorant, and a flavoring / flavoring agent.
Examples of the binder include water, ethanol, propanol, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, hydroxypropylcellulose, hydroxypropyl starch, methylcellulose, ethylcellulose, shellac, calcium phosphate, polyvinylpyrrolidone and the like. It is done.
Examples of the disintegrant include dry starch, sodium alginate, agar powder, sodium hydrogen carbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, and lactose.
Examples of the lubricant include purified talc, stearate, borax, and polyethylene glycol.
Examples of the colorant include titanium oxide and iron oxide.
Examples of the flavoring / flavoring agent include sucrose, orange peel, citric acid, tartaric acid and the like.

As the oral solution, for example, additives such as a taste-masking / flavoring agent, a buffering agent, a stabilizer and the like can be added and produced by a conventional method. Here, the additive is not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected according to the purpose.
Examples of the flavoring / flavoring agent include sucrose, orange peel, citric acid, tartaric acid and the like.
Examples of the buffer include sodium citrate.
Examples of the stabilizer include tragacanth, gum arabic, and gelatin.

  The hair-restoring agent and anti-obesity agent of the present invention are suitably applied to humans, but can also be applied to animals other than humans as long as their respective effects are exhibited. .

(Cosmetics)
The cosmetic contains at least one of the hair restorer and anti-obesity agent of the present invention, and further contains other components as necessary.
The cosmetic of the present invention can improve at least one of the hair-growth effect and the anti-obesity effect through the testosterone 5α-reductase activity inhibitory action and cAMP phosphodiesterase activity inhibitory action by the lactic acid bacteria fermentation product of the sake lees extract.

  The content of at least one of the hair restorer and anti-obesity agent is not particularly limited and may be appropriately selected depending on the intended use. However, the content is high with respect to the cosmetic. Preferably, 10 mg / g or more is preferable, 200 mg / g or more is more preferable, and 500 mg / g or more is particularly preferable.

<Other ingredients>
The other components are not particularly limited as long as they are used in cosmetics, and can be appropriately selected according to the purpose. For example, astringents, bactericides, antibacterial agents, ultraviolet absorbers, humectants, Examples include cell activators, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances and the like. These may be used alone or in combination of two or more.
There is no restriction | limiting in particular as content of the said other component, According to the objective, it can select suitably.

(Food)
The food or drink contains at least one of the hair restorer of the present invention and an anti-obesity agent, and further contains other components as necessary.
The food and drink of the present invention can improve at least one of the hair-growth effect and the anti-obesity effect through the testosterone 5α-reductase activity inhibitory action and the cAMP phosphodiesterase activity inhibitory action by the lactic acid bacteria fermentation product of the sake lees extract. In the present invention, food and drink means a wide range of food and drink aimed at promoting hair growth and preventing obesity.

  There is no restriction | limiting in particular as said food-drinks, According to the objective, it can select suitably, For example, drinks, such as a tea drink, a soft drink, a carbonated drink, a nutritive drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet , Shaved ice and other frozen desserts; noodles such as buckwheat, udon, harsame, gyoza skin, sushi mai, Chinese noodles, instant noodles; rice cakes, candy, gum, chocolate, tablet confectionery, snacks, biscuits, jelly, jam, cream, Sweets such as baked goods and bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red sea bream, scallop, abalone, sea urchin, sea bream, tocobushi, etc .; Processed fish and livestock products such as ham and sausage; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, shii Oil and fat processed foods such as toning, whipped cream, dressing; seasonings such as sauce, sauce; curry, stew, parent and child rice bowl, rice bowl, miscellaneous cooking, Chinese rice bowl, bowl, tempura, eel rice cake, hayashi rice, oden, marbodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger and meatballs; various forms of health foods and dietary supplements.

  The content of at least one of the hair restorer and the anti-obesity agent is not particularly limited and may be appropriately selected depending on the application. However, the content is high with respect to the food and drink. Preferably, 10 mg / g or more is preferable, 200 mg / g or more is more preferable, and 500 mg / g or more is particularly preferable.

<Other ingredients>
There is no restriction | limiting in particular as said other component, According to the objective, it can select suitably.

<Application>
Since the hair-restoring agent, anti-obesity agent, cosmetics, and food and drink of the present invention are excellent in hair-restoring effect and anti-obesity effect, the hair-restoring agent, anti-obesity agent, and cosmetics containing at least one of these, and It can be suitably used for food and drink.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

(Preparation Example 1)
As the sake lees, white peony liquor (manufactured by white peony brewing) was used. 5 g of the sake lees were suspended in 100 g of water to obtain a liquor extract.

(Preparation Example 2)
The prepared sake lees extract is autoclaved with a high-pressure steam sterilizer (device name: MLS-3571-PJ, manufactured by Panasonic Corporation) at 110 ° C. for 5 minutes, sterilized and then cooled. After cooling to about room temperature, 0.1 g of lactic acid bacteria (species name: Lactobacillus plantarum 22A-3, in-house culture) is added, and shaking culture is performed at 30 ° C. for 16 to 24 hours. After completion of the culture, the mixture was sterilized by autoclaving again at 110 ° C. for 5 minutes and filtered through diatomaceous earth to obtain a lactic acid bacteria fermentation product (liquid).
The obtained fermented lactic acid bacteria was freeze-dried with a freeze-dryer (device name: FDU-2100, manufactured by EYELA) to be powdered.

  Next, using the powdered lactic acid bacteria fermented product of the sake lees extract (test sample), “hair growth action (testosterone 5α-reductase activity inhibitory effect)” and “anti-obesity action (cAMP phosphodiesterase activity) are performed as follows. Inhibitory effect) ”was evaluated. The results are shown in Tables 3 to 4 below.

Example 1
<Hair growth action>
<< Testosterone 5α-reductase activity inhibition test >>
4.2 mg / mL testosterone (manufactured by Wako Pure Chemical Industries) dissolved in propylene glycol (manufactured by Wako Pure Chemical Industries, Ltd.) 20 μL, 1 mg / mL NADPH containing V-bottom test tube with lid (manufactured by Asahi Glass Co., Ltd.) A mixed solution of 825 μL of 5 mmol / L Tris-HCl buffer (Wako Pure Chemical Industries, Ltd., pH 7.13) was prepared. Sake lees extract dissolved in purified water so that the concentration of the mixed solution is as shown in Table 3, or 80 μL of lactic acid bacteria fermentation product of sake lees extract, and crude enzyme solution S-9 (manufactured by Oriental Yeast Co., Ltd.) After 75 μL was added and mixed and reacted at 37 ° C. for 60 minutes, 1 mL of methylene chloride (manufactured by Wako Pure Chemical Industries, Ltd.) was added to stop the reaction. Centrifugation was performed at 1,600 × g for 10 minutes using a centrifuge (device name: KUBOTA7820, manufactured by Kubota Corporation), and the methylene chloride layer was subjected to gas chromatography analysis under the following conditions.

-Gas chromatography conditions-
-Equipment used: Shimadzu GC-2010
Column: DB-1701 (diameter: 0.53 mm × 30 m, film thickness: 1.0 mm)
Column / injection temperature: 240 ° C / 300 ° C
・ Detector: FID
・ Carrier gas: Nitrogen gas

  In order to calculate the conversion rate of testosterone to dihydrotestosterone and 3α-androstanediol, a standard sample solution having a known content of testosterone, dihydrotestosterone and 3α-androstanediol was subjected to gas chromatography analysis, and each compound was analyzed. The peak area of was determined. With respect to each peak area of the prepared test sample (sample added with sake lees extract or sample added with lactic acid bacteria fermentation product of sake lees extract), the relative ratio to the peak area of the standard sample solution was determined according to the following formula 1. From the obtained relative ratio, the conversion rate of the test sample to dihydrotestosterone and 3α-androstanediol was determined according to the following formula 2. Based on the conversion rate, the testosterone 5α-reductase activity inhibition rate was determined according to the following formula 3 and used as an index of the hair-growth effect. In addition, in order to calculate an inhibition rate, it carried out like Example 1 except having added purified water instead of the said test sample as a control test. The results are shown in Table 3 below.

Relative ratio = peak area of test sample / peak area of standard sample solution

Conversion rate (%) = (A + B) / (A + B + C) × 100 Equation 2
A: Relative ratio of 3α-androstanediol B: Relative ratio of dihydrotestosterone C: Relative ratio of testosterone

Testosterone 5α-reductase activity inhibition rate (%) = (1−E / D) × 100 Formula 3
D: Conversion rate in the control test E: Conversion rate when the test sample is added

  From the results shown in Table 3, the hair-growth effect by inhibiting testosterone 5α-reductase activity was confirmed together with the sake lees extract and the fermented lactic acid bacteria of the sake lees extract. It was found that the fermented lactic acid bacteria of the sake lees extract had about three times the activity of the sake lees extract under the condition of the test sample concentration of 3,000 μg / mL.

(Example 2)
<Anti-obesity action>
<< cAMP phosphodiesterase activity inhibition test >>
To a V-bottom test tube with a lid (manufactured by Asahi Glass Co., Ltd.), 5 mg / L magnesium chloride-containing 50 mmol / L Tris-HCl buffer (Wako Pure Chemical Industries, Ltd., pH 7.5) in 0.2 mL, 2.5 mg / ML bovine serum albumin solution (trade name: albumin derived from bovine serum, product number: A2153, Sigma-Aldrich Japan GK) 0.1 mL, 0.1 mg / mL phosphodiesterase solution (trade name: Phosphoesterase cyclic Nucleotide Activator-Defective, Fromm Bovine Heart, Crude (manufactured by Sigma Aldrich Japan GK) 0.1 mL, and sake lees extract or 0.05 mL of lactic acid bacteria fermentation product of sake lees extract were added, and the mixed solution was reacted at 37 ° C. for 5 minutes. After the reaction, 0.05 mL of 0.5 mg / mL cyclic AMP (cAMP) solution was added to the mixed solution, reacted at 37 ° C. for 60 minutes, then boiled on a boiling water bath for 3 minutes to stop the reaction. It was. Centrifugation was performed at 2,260 × g for 10 minutes at 4 ° C. with a centrifuge (device name: KUBOTA7820, manufactured by Kubota Corporation), and the supernatant was subjected to HPLC analysis under the following conditions. Further, a blank test was performed in the same manner as described above.

-Conditions for HPLC chromatography-
-Equipment used: Wakosil C18-ODS 5 μm
-Mobile phase: 1 mM TBAP in 25 mM KH ₂ PO ₄ : CH ₃ CN
= 90: 10
・ Flow rate: 1.0 mL / min
・ Detector: UV-visible spectrophotometer (measurement wavelength: 260 nm)
-ATTEN: 128

  In order to calculate the inhibition rate of the phosphodiesterase activity by the test sample, the standard sample solution with a known cAMP content, the sample solution with no test sample added, and the cAMP peak area contained in the sample solution with the test sample added, respectively, It calculated | required as (A), (B1), (B2). From the obtained results, the cAMP degradation rate (C) when no test sample was added and the cAMP degradation rate (D) when the test sample was added were calculated based on the following formulas 4 and 5. The results are shown in Table 4 below.

-Degradation rate of cAMP without addition of test sample (C,%) = (1-B1 / A) x 100

-Decomposition rate of cAMP at the time of addition of test sample (D,%) = (1-B2 / A) x 100

  Based on the respective degradation rates (C, D) calculated from the above formulas 4 and 5, the cAMP phosphodiesterase activity inhibition rate (%) is calculated by the following formula 6, and the results are shown in Table 4.

CAMP phosphodiesterase activity inhibition rate (%) = (1−D / C) × 100 Formula 6

  From the results shown in Table 4, it was found that the lactic acid bacteria fermented product of the sake lees extract had an inhibitory effect on cAMP phosphodiesterase activity.

(Formulation Example 1)
-Hair tonic-
A hair tonic having a hair growth action having the following composition was produced by a conventional method.
・ Pyridoxine hydrochloride ... 0.1g
・ Resorcin ・ ・ ・ 0.01g
・ D-pantothenyl alcohol ... 0.1g
・ Dipotassium glycyrrhizinate ... 0.1g
・ L-Menthol 0.05g
・ 1,3-Butylene glycol ・ ・ ・ 4.0g
・ Carrot extract ... 0.5g
・ Ethanol ... 25.0g
・ Lactobacillus fermented product of liquor extract of Preparation Example 2 (liquid) ... 40mL
-Fragrance-appropriate amount-Purified water-remaining amount (total amount is 100.0 mL)

(Formulation Example 2)
-Shampoo-
A shampoo (cream shampoo) having a hair-growing action having the following composition was produced by a conventional method.
・ Polyoxyethylene alkyl ether sodium sulfate 30.0 g
・ Polyoxyethylene alkyl ether ammonium sulfate ... 20.0g
・ Palm oil fatty acid amidopropyl betaine ... 6.0g
・ Palm oil fatty acid modiethanolamide: 4.0 g
・ Ethylene glycol distearate ... 2.0g
・ Preservative (methyl paraoxybenzoate) ... 0.15g
・ Muguroji extract ... 0.2g
・ Cotton extract ... 0.5g
・ Oat extract ... 0.3g
・ Rosemary extract 0.5g
・ Perfume 0.01g
・ 1,3-Butylene glycol ・ ・ ・ 3.0g
-Lactic acid bacteria fermented product (liquid) of the sake lees extract of Preparation Example 2 (remaining amount (total amount is 100.0 mL))

(Formulation Example 3)
-Rinse-
A rinse having a hair-growth effect having the following composition was produced by a conventional method.
・ Stearyltrimethylammonium chloride 1.5g
・ Polyoxyethylene cetyl ether ... 1.0g
・ Cetyl alcohol: 2.0g
・ Octyldodecanol ・ ・ ・ 1.0g
・ Cationized cellulose: 0.5g
・ Propylene glycol ... 5.0g
・ Lactobacillus fermented product of liquor extract of Preparation Example 2 (liquid) 20 mL
・ Muguroji extract ... 0.2g
・ Cotton extract ... 0.5g
・ Oat extract ... 0.3g
・ Rosemary extract 0.5g
・ Fragrance ... 3.0g
・ Purified water: remaining amount (the total amount is 100.0 mL)

  Since the hair-restoring agent, anti-obesity agent, cosmetics, and food and drink of the present invention are excellent in hair-restoring action and anti-obesity action, the hair-restoring agent, anti-obesity agent, and cosmetics containing at least one of these, and It can be suitably used for food and drink.

Claims (6)

  A hair restorer comprising a fermented lactic acid bacterium obtained by fermenting a liquor extract with lactic acid bacteria.   The hair restorer according to claim 1, wherein the lactic acid bacterium is Lactobacillus plantarum.   An anti-obesity agent comprising a fermented lactic acid bacterium obtained by fermenting a liquor extract with lactic acid bacteria.   The antiobesity agent according to claim 3, wherein the lactic acid bacterium is Lactobacillus plantarum.   It is at least any selected from the object for hair growth containing the hair growth agent in any one of Claim 1 to 2, and the anti-obesity agent containing the anti-obesity agent in any one of Claim 3 to 4. And cosmetics.   It is at least any selected from the object for hair growth containing the hair growth agent in any one of Claim 1 to 2, and the anti-obesity agent containing the anti-obesity agent in any one of Claim 3 to 4. Food and drink.