JP2018171370A - Biological diagnostic apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a biological diagnostic apparatus capable of appropriately acquiring both a blood stream and a deformation amount of a biological tissue.SOLUTION: A coherent light source 2 emits measuring light. An irradiation optical system 10 irradiates an area to be examined of a living tissue with the measuring light emitted by the coherent light source. A light receiving element 40 receives reflection light of the measuring light reflected by the area to be examined. A control part 50 acquires a blood stream image showing a two-dimensional distribution at blood stream speed in the area to be examined from a temporal change of laser speckle in each of a plurality of pixels continuously photographed by the light receiving element 40. The control part 50 acquires laser speckle in an image photographed by the light receiving element 40 at a first time and a deformation amount of the living tissue in the area to be examined from each phase information of the laser speckle in the image photographed by the light receiving element 40 at a second time different from the first time.SELECTED DRAWING: Figure 1

Description

  The present disclosure relates to a biodiagnosis apparatus used for diagnosing a living tissue.

  2. Description of the Related Art Conventionally, a blood flow imaging apparatus that images a two-dimensional distribution of blood flow velocity of a living tissue is known. For example, the blood flow imaging device described in Patent Document 1 expands measurement light, which is a laser beam, and irradiates the fundus, and forms reflected light from the fundus as a laser speckle on a two-dimensional image sensor. A blood flow map is generated from the time change of the laser speckle generated on the image plane.

Japanese Patent No.4102888

  There are cases where it is desired to use both the blood flow of the living tissue and the deformation amount of the living tissue for diagnosis. For example, in glaucomatous eyes, in addition to a decrease in blood flow in the optic nerve head, there is a report that plastic deformation tends to occur in the fundus tissue. Conventionally, when both blood flow and deformation amount in a living tissue are used for diagnosis, a doctor uses a blood flow imaging device and a device that measures the deformation amount of the tissue (for example, an optical coherence tomometer that captures a tomographic image of a tissue) Etc.) was required to make a diagnosis.

  A typical object of the present disclosure is to provide a biodiagnosis device that can appropriately acquire both blood flow and deformation amount of a living tissue.

  A biodiagnosis device provided by an exemplary embodiment of the present disclosure includes a coherent light source that emits measurement light that is coherent light, and irradiation that irradiates the examination target region of the biological tissue with the measurement light emitted from the coherent light source. An optical system; a light receiving element that receives the reflected light of the measurement light reflected by the inspection target region; and a control unit that controls the biodiagnosis apparatus, and the control unit is continuous by the light receiving element. A blood flow image showing a two-dimensional distribution of blood flow velocity in the examination region is acquired from a time change of the laser speckle in each of the plurality of images photographed in the above manner, and photographed at the first time by the light receiving element. Each of the laser speckle in the image and the laser speckle in the image taken by the light receiving element at a second time different from the first time. From the phase information to obtain the amount of deformation of the biological tissue in the inspection region.

  The biodiagnosis device according to the present disclosure can appropriately acquire both the blood flow and the deformation amount of the living tissue.

1 is a diagram illustrating a schematic configuration of a biodiagnosis device 1. FIG. It is a figure which shows typically an example of the state by which measurement light is irradiated to the fundus F when acquiring a blood-flow image. It is a figure which shows typically an example of the state by which measurement light is irradiated to the fundus F when acquiring the in-plane deformation amount of a biological tissue. It is explanatory drawing for demonstrating the optical path of the measurement light at the time of acquiring the out-of-plane deformation amount of a biological tissue. It is a figure which shows typically the blood-flow image 61, the deformation distribution image 62, and the duplication image 63. FIG. It is a figure which shows schematic structure of the optical system of the biodiagnosis apparatus which concerns on a modification.

<Overview>
A biodiagnosis device exemplified in the present disclosure includes a coherent light source, an irradiation optical system, a light receiving element, and a control unit. The coherent light source emits measurement light that is coherent light. The irradiation optical system irradiates the measurement target region of the living tissue with the measurement light emitted from the coherent light source. The light receiving element receives the reflected light of the measurement light reflected by the inspection target region. The control unit can acquire a blood flow image indicating a two-dimensional distribution of blood flow velocity in the examination region from the time change of the laser speckle in each of the plurality of images continuously captured by the light receiving element. In addition, the control unit determines from the phase information of the laser speckle in the image photographed by the light receiving element at the first time and the laser speckle in the image photographed by the light receiving element at the second time different from the first time. The amount of deformation of the living tissue in the examination region can be acquired. Therefore, the biodiagnosis device in the present disclosure can appropriately acquire both the blood flow and the deformation amount of the living tissue.

  In the biodiagnosis device according to the present disclosure, it is easy to share at least one of various configurations such as a coherent light source and an irradiation optical system when acquiring a blood flow image and when acquiring a deformation amount. Therefore, the apparatus configuration is also simplified. However, you may provide separately, without sharing a part of each structure. For example, the light receiving element used when acquiring the blood flow image and the light receiving element used when acquiring the deformation amount may be a common light receiving element or different light receiving elements.

  The irradiation optical system may include a branching optical element and a branching light guiding optical system. The branch optical element branches the measurement light emitted from the coherent light source. The branch light guiding optical system guides each of the plurality of measurement lights branched by the branch optical element toward the living tissue. When acquiring the amount of deformation of the living tissue, the control unit uses the branched light guide optical system to each of the plurality of measurement lights branched by the branch optical element at each of the first time and the second time. An image may be acquired by overlapping and irradiating the area. In this case, the phase information is appropriately acquired by interference of a plurality of measurement lights irradiated on the same inspection target region. A deformation amount of the living tissue between the first time and the second time is acquired from the acquired phase information. Therefore, the deformation amount of the living tissue is appropriately acquired.

  As an example, in the present disclosure, the deformation amount in the in-plane direction (direction along the surface) of the surface (irradiation surface) irradiated with the measurement light is acquired by the two-beam interference measurement method. Specifically, in the present disclosure, two branched measurement lights are irradiated in an overlapping manner from two directions that are symmetric with respect to the normal line of the measurement surface. Although the incident directions of the two measurement lights are different, the incident angles with respect to the irradiation surface are the same. When the reflected light of the measurement light reflected by the irradiation surface is received by the light receiving element, a laser speckle due to the interference of the two measurement lights appears in the image taken by the light receiving element. A control part acquires a fringe image (specklegram) from the absolute value of the difference of the speckle intensity | strength in each of 1st time and 2nd time, and calculates deformation amount based on the phase difference obtained from a fringe image.

  In addition, when irradiating a plurality of measurement light beams in an overlapping manner, the plurality of measurement light beams do not have to be strictly overlapped, and at least a part of them may be overlapped. In this case, a region irradiated with a plurality of measurement light beams is an inspection target region from which the deformation amount is acquired.

  The biodiagnosis device may include an optical path change drive unit. The optical path change driving unit changes at least one optical path of the branched measurement light by driving the branched light guiding optical system. When acquiring the deformation amount of the living tissue, the control unit may control driving of the optical path change driving unit and irradiate each of the plurality of measurement lights on the same examination target region. In addition, when acquiring a blood flow image, the control unit may control driving of the optical path change driving unit and irradiate each of a plurality of examination target regions that do not overlap each other with a plurality of measurement lights.

  In this case, when acquiring the deformation amount, a plurality of measurement lights are applied in an overlapping manner, and when acquiring a blood flow image, the plurality of measurement lights are applied to different regions. As a result, both the deformation amount and the blood flow image are acquired appropriately. Furthermore, when a blood flow image is acquired, a plurality of measurement lights are emitted without overlapping, so that a blood flow image in a wide area is acquired.

  However, the biodiagnosis device may not include the optical path change drive unit. In this case, when acquiring the deformation amount, the control unit irradiates a plurality of measurement lights in an overlapping manner. On the other hand, when acquiring a blood flow image, the control unit may irradiate the living tissue with one of the branched measurement lights or with the measurement light in an unbranched state. Even in this case, both the deformation amount and the blood flow image are appropriately acquired.

  The biodiagnosis device may include a branching optical system, a branching light guiding optical system, and a reference optical system. The branch optical element branches the measurement light emitted from the coherent light source. The branched light guide optical system guides at least one of the plurality of measurement lights branched by the branch optical element to the living tissue. The reference optical system guides the measurement light different from the measurement light guided to the living tissue among the plurality of measurement lights branched by the branch optical element to the light receiving element as the reference light. When acquiring the deformation amount of the biological tissue, the control unit guides the reflected light of the measurement light irradiated to the biological tissue by the branched light guiding optical system and the reference optical system at each of the first time and the second time. An image obtained by interference light with the reference light is acquired. In this case, the phase information is appropriately acquired by the interference between the reflected light of the measurement light and the reference light. A deformation amount of the living tissue between the first time and the second time is acquired from the acquired phase information. Therefore, the deformation amount of the living tissue is appropriately acquired.

  As an example, in the present disclosure, the amount of deformation in the out-of-plane direction (direction perpendicular to the surface) of the surface irradiated with the measurement light is acquired by causing the reflected light of the measurement light and the reference light to interfere with each other. Specifically, in the present disclosure, laser speckle due to interference between the reflected light of the measurement light and the reference light appears in the image captured by the light receiving element. A control part acquires a fringe image (specklegram) from the absolute value of the difference of the speckle intensity | strength in each of 1st time and 2nd time, and calculates deformation amount based on the phase difference obtained from a fringe image.

  The biodiagnosis device may include an optical path change drive unit. The optical path change driving unit changes at least one optical path of the branched measurement light by driving the branched light guiding optical system. When acquiring the deformation amount of the living tissue, the control unit controls the driving of the optical path change driving unit, irradiates one of the plurality of measurement lights branched by the branching optical element to the examination target region, and is branched. Other measurement light may be guided to the reference optical system. In addition, when acquiring a blood flow image, the control unit may control driving of the optical path change driving unit and irradiate each of a plurality of examination target regions that do not overlap each other with a plurality of measurement lights.

  In this case, when acquiring the deformation amount, both the reflected light of the measurement light and the reference light are guided to the light receiving element, and when acquiring the blood flow image, a plurality of measurement lights are irradiated to different regions. As a result, both the deformation amount and the blood flow image are acquired appropriately. Furthermore, when a blood flow image is acquired, a plurality of measurement lights are emitted without overlapping, so that a blood flow image in a wide area is acquired.

  However, the biodiagnosis device may not include the optical path change drive unit. In this case, the control unit guides the reflected light of the measurement light and the reference light to the light receiving element when acquiring the deformation amount. On the other hand, when acquiring a blood flow image, the control unit may irradiate the living tissue with one of the branched measurement lights or with the measurement light in an unbranched state. Even in this case, both the deformation amount and the blood flow image are appropriately acquired.

  The control unit may generate a deformation amount distribution image indicating a two-dimensional distribution of the deformation amount of the living body. The control unit may cause the display unit to display the deformation amount distribution image superimposed on or side by side with the blood flow image acquired for the same examination target region. In this case, the doctor can easily make a diagnosis by grasping the two-dimensional distribution of blood flow and the two-dimensional distribution of deformation.

<Embodiment>
Hereinafter, exemplary embodiments of the present disclosure will be described with reference to the drawings. The biodiagnosis device 1 of the present embodiment is used by a doctor to diagnose the fundus F of the subject's eye E, which is a living tissue. However, at least a part of the technique exemplified in the present disclosure can be applied to a case where a blood flow image of a living tissue other than the fundus F is generated.

  With reference to FIG. 1, a schematic configuration of the biodiagnosis apparatus 1 of the present embodiment will be described. The biodiagnosis apparatus 1 includes a coherent light source 2, an irradiation optical system 10, a light receiving optical system 20, a reference optical system 30 (described later with reference to FIG. 4), a light receiving element 40, and a control unit 50. In the biodiagnosis device 1 of the present embodiment, the objective lens 3 is shared between the irradiation optical system 10 and the light receiving optical system 20. In FIG. 1, the irradiation optical system 10 and the light receiving optical system 20 are separately illustrated in order to facilitate understanding of the configuration of the optical system. As a result, the shared objective lens 3 and subject eye E are shown in the irradiation optical system 10 and the light receiving optical system 20, respectively. However, the objective lens 3 may not be shared between the irradiation optical system 10 and the light receiving optical system 20. In FIG. 1, the optical axis of the measurement light is indicated by a dotted line.

  The coherent light source 2 emits coherent light as measurement light. In the present embodiment, a laser light source (for example, a semiconductor laser light source) that emits laser light having coherence is used as the coherent light source 2.

  The irradiation optical system 10 irradiates the measurement light emitted from the coherent light source 2 onto the inspection target region of the living tissue (in this embodiment, the inspection target region that extends in the two-dimensional direction on the fundus F of the eye E of the subject). . The irradiation optical system 10 in this embodiment includes a branching optical element 11, a branching light guiding optical system 12, and an objective lens 3 in order from the upstream side of the measuring light optical path (the side close to the coherent light source 2). The irradiation optical system 10 may be provided with other optical elements (for example, a collimating lens that collimates the light beam of the measurement light emitted from the coherent light source 2), but this description is omitted.

  The branch optical element 11 branches the measurement light emitted from the coherent light source 2. As an example, in this embodiment, a beam splitter is used as the branching optical element 11. The measurement light is split into two measurement light beams 22A and 22B by a beam splitter. Needless to say, the configuration of the branch optical element 11 can be changed. For example, a half mirror, a coupler, or the like that branches light may be used as the branching optical element 11.

  The branched light guiding optical system 12 guides at least one of the plurality of measurement lights 22A and 22B branched by the branched optical element 11 to a living tissue (in this embodiment, the fundus F of the eye E of the subject). Shine. In the example shown in FIG. 1, the branched light guide optical system 12 guides two measurement lights 22A and 22B to the living tissue. However, as shown in FIG. 4, only one of the two measurement lights 22A and 22B may be guided to the living tissue (details will be described later). As an example, the branched light guiding optical system 12 of the present embodiment includes two galvanometer mirrors 13A and 14A that guide the measurement light 22A to the living tissue, and two galvanometer mirrors 13B that guide the measurement light 22B to the living tissue. 14B. The measurement light 22A is reflected by the galvanometer mirror 13A, then reflected by the galvanometer mirror 14A, and guided to the living tissue. The measurement light 22B is reflected by the galvanometer mirror 13B, then reflected by the galvanometer mirror 14B, and guided to the living tissue. The objective lens 3 irradiates the living tissue with the measurement light guided by the branched light guide optical system 12.

  Furthermore, the biodiagnosis apparatus 1 includes optical path change drive units 16 and 17. The optical path change driving units 16 and 17 change the at least one optical path of the branched measurement lights 22A and 22B by driving the branched light guiding optical system 12. As an example, the optical path change drive units 16 and 17 of this embodiment include a drive unit 16A that drives the galvano mirror 13A, a drive unit 17A that drives the galvano mirror 14A, a drive unit 16B that drives the galvano mirror 13B, and a galvano A drive unit 17B for driving the mirror 14B is used. Needless to say, the specific configurations of the branched light guiding optical system 12 and the optical path changing drive units 16 and 17 can be changed as appropriate.

  The light receiving optical system 20 guides the reflected light of the measurement light reflected by the examination target region of the living tissue to the light receiving element 40. In the light receiving optical system 20 of the present embodiment, the reflected light of the measurement light passes through the objective lens 3 and the relay lens 21 and enters the imaging surface of the light receiving element 40.

  The light receiving element 40 receives the reflected light of the measurement light guided by the light receiving optical system 20 (in the example shown in FIGS. 4 and 6, the reflected light of the measurement light and the interference light of the reference light), thereby living tissue The area to be inspected is photographed. In the present embodiment, a two-dimensional image sensor (for example, a two-dimensional CCD sensor in which a plurality of light receiving elements are arranged on a two-dimensional plane) is used as the light receiving element 40.

  The control unit 50 includes a controller (CPU) and a storage device, and manages various processes in the biological diagnostic apparatus 1. As an example, the control unit 50 of the present embodiment is electrically connected to the coherent light source 2 and controls the emission of measurement light by the coherent light source 2. The control unit 50 is electrically connected to the optical path change driving units 16 and 17 and controls the driving of the optical path change driving units 16 and 17. Furthermore, the control unit 50 can execute a blood flow image acquisition process of a living tissue and a deformation amount acquisition process of the living tissue.

  The control unit 50 may be provided in a case of the biodiagnosis apparatus 1 including various optical systems, or may be provided in a case different from the case including various optical systems. For example, the control unit of the PC may be used as the control unit 50 of the biodiagnosis apparatus 1 by connecting a housing including various optical systems to a personal computer (hereinafter referred to as “PC”).

<Acquisition of blood flow image>
A method for acquiring a blood flow image in the present embodiment will be described. The blood flow image 61 (see FIG. 5) in the present embodiment is an image showing a two-dimensional distribution of blood flow velocity in a living body inspection target region. The control unit 50 generates a blood flow image 61 from the time change of the output signal of each corresponding pixel of a plurality of images in which the same examination target region is continuously captured by the light receiving element 40. When the living tissue is irradiated with coherent light, the scattered light interferes with each other, and a random spot pattern called laser speckle is formed. Laser speckle is dynamically changed by moving scattering particles (for example, blood cells) in a living tissue. The control unit 50 quantifies the time change of the laser speckle in a plurality of images taken continuously, by processing the output signal in each pixel, and generates a blood flow image 61. In this embodiment, an average blur rate (MBR) is used as an example of a value for quantifying the blood flow velocity. When the blood flow is slow, the speckle spot pattern of the photographed image moves slowly, so the average blur rate becomes small. On the other hand, when the blood flow is fast, the speckle speckled pattern moves fast, and the average blur rate increases. Accordingly, a two-dimensional distribution of blood flow velocity can be generated based on the average blur rate.

  As shown in FIG. 2, when acquiring the blood flow image 61, the control unit 50 controls the driving of the optical path change driving units 16 and 17 so that each of the plurality of measurement lights 22A and 22B does not overlap each other. Irradiation is performed on each of a plurality (two in this embodiment) of inspection target areas 5A and 5B. The measurement light reflected by the plurality of inspection target regions 22A and 22B is incident on the light receiving element 40. Accordingly, a blood flow image 61 in a wider area is acquired as compared with the case where the measurement light is irradiated only on one examination target area.

<Acquisition of in-plane deformation amount>
A method for obtaining the in-plane deformation amount in the present embodiment will be described. The in-plane deformation amount is a deformation amount in the in-plane direction (direction along the surface) of the irradiation surface irradiated with the measurement beams 22A and 22B. As shown in FIG. 3, when acquiring the in-plane deformation amount, the control unit 50 controls the driving of the optical path change driving units 16 and 17 so that each of the plurality of measurement beams 22A and 22B is subjected to the same inspection. Irradiation is performed so as to overlap the target region 5C. Here, the biodiagnosis device 1 of the present embodiment causes the two measurement beams 22A and 22B to enter the inspection target region 5C from different directions, while the incident angles of the two measurement beams 22A and 22B with respect to the irradiation surface (FIG. 3). Are the same.

  When the reflected light of the measurement light reflected by the inspection target region 5C is received by the light receiving element 40, an image photographed by the light receiving element 40 (hereinafter referred to as “speckle image”) includes two measurement lights 22A, Laser speckle due to 22B interference appears. Speckle images are taken at least twice at different times. A deformation amount is calculated from a plurality of speckle images photographed at different times.

  As an example, when speckle images are taken in each of a state in which the intraocular pressure is increased by applying pressure to the subject's eye E (first time) and a state in which the pressure is released (second time), etc. Can be considered. In this case, the deformation amount of the fundus F before and after releasing the pressure is acquired. If the intraocular pressure continues to be high due to glaucoma or the like, plastic deformation may occur in living tissue such as the fundus F, and the deformed shape may be less likely to return compared to a healthy eye. Therefore, when the deformation amount of the fundus F before and after the pressure is released is acquired, the doctor can grasp the degree of plastic deformation of the fundus F and may be useful for diagnosis of glaucoma and the like. However, it goes without saying that the deformation amount may be acquired for another diagnosis.

The control unit 50 acquires a fringe image from the absolute value of the difference in speckle intensity in a plurality of speckle images taken at different times. In the fringe image, one interference fringe appears every time the phase difference between the optical paths of the two measurement beams 22A and 22B changes by 2π due to deformation of the irradiation surface. The control unit 50 acquires the phase difference from the fringe image, and calculates the in-plane deformation amount from (Equation 1). Here, Δφ is the amount of change in the phase difference between the optical paths of the two measuring beams 22A and 22B before and after deformation, u is the amount of in-plane deformation, λ is the wavelength of the measuring beams 22A and 22B, and θ is the incidence of the measuring beams 22A and 22B. Is an angle.

<Acquisition of out-of-plane deformation>
A method for acquiring the out-of-plane deformation amount in the present embodiment will be described. The out-of-plane deformation amount is a deformation amount in the out-of-plane direction (direction perpendicular to the surface) of the irradiation surface irradiated with the measurement light (measurement light 22B in the present embodiment).

  FIG. 4 is an explanatory diagram for explaining the optical paths of the measurement beams 22A and 22B when acquiring the out-of-plane deformation amount. Even when the out-of-plane deformation amount is acquired, the reflected light of the measurement light reflected by the examination target region of the living tissue passes through the same optical path as the optical path shown in the light receiving optical system 20 in FIG. Light is received by the light receiving element 40. However, in FIG. 4, the optical path of the reflected light guided by the light receiving optical system 20 is omitted for easy understanding of the drawing.

  As shown in FIG. 4, when acquiring the out-of-plane deformation amount, the control unit 50 irradiates one of the two branched measurement lights 22A and 22B with the measurement light 22B on the examination target region in the living tissue. . In addition, when acquiring the out-of-plane deformation amount, the control unit 50 controls the driving of the optical path change driving units 16 and 17 to supply the reference optical system 30 with the measurement light 22A different from the measurement light 22B irradiated to the living tissue. Light guide. The reference optical system 30 guides the measurement light 22A to the light receiving element 40 as reference light that interferes with the reflected light of the measurement light 22B. As an example, the reference optical system 30 of the present embodiment includes a reference surface that generates reference light by reflecting the measurement light 22A. The measurement light 22A is reflected by the reference surface and then received by the light receiving element 40 as reference light. The reference optical system 30 may include an optical path length difference adjustment drive unit (for example, a drive unit that moves the reference surface along the optical path) that adjusts the optical path length difference between the reflected light of the measurement light 22B and the reference light. Good.

  When the reflected light of the measurement light 22 </ b> B guided by the light receiving optical system 20 and the reference light guided by the reference optical system 30 are received by the light receiving element 40, an image photographed by the light receiving element 40 (hereinafter, “ In the speckle image), laser speckle due to interference between reflected light and reference light appears. The speckle image is taken at least twice at different timings as in the case of measuring the in-plane deformation amount.

The control unit 50 acquires a fringe image from the absolute value of the difference in speckle intensity in a plurality of speckle images taken at different times. The control unit 50 acquires the phase difference from the fringe image, and calculates the out-of-plane deformation amount w from (Equation 2). Here, Δφ is the amount of change before and after the phase difference between the reflected light of the reference light 22A and the measurement light 22B, and λ is the wavelength of the reference light 22A and the measurement light 22B.

  As described above, the biological diagnosis apparatus 1 of the present embodiment appropriately controls each of the blood flow image, the in-plane deformation amount, and the out-of-plane deformation amount by controlling the driving of the optical path change driving units 16 and 17. Can be acquired.

<Display of various images>
With reference to FIG. 5, the display method of the various images in the biodiagnosis apparatus 1 of this embodiment is demonstrated. As described above, the biodiagnosis device 1 of the present embodiment can generate the blood flow image 61. In addition, the biodiagnosis device 1 according to the present embodiment can generate a deformation amount distribution image 62 showing a two-dimensional distribution of the deformation amount of the living body. The control unit 50 may generate the deformation amount distribution image 62 by using both the in-plane deformation amount and the out-of-plane deformation amount, or based on one of the in-plane deformation amount and the out-of-plane deformation amount. May be generated.

  As shown in FIG. 5, the biodiagnosis apparatus 1 of the present embodiment can display the generated blood flow image 61 and the deformation amount distribution image 62 side by side on the screen of the display unit. In the present embodiment, the control unit 50 can also generate an overlapping image 63 that is an image obtained by overlapping the blood flow image 61 and the deformation amount distribution image 62 and display the overlapping image 63 on the display unit. Therefore, the doctor can more easily grasp the two-dimensional distribution of the blood flow and the two-dimensional distribution of the deformation amount for diagnosis.

<Transformation example>
The technology disclosed in the above embodiment is merely an example. Therefore, it is possible to change the technique exemplified in the above embodiment. For example, the biodiagnosis device 1 may acquire only one of the in-plane deformation amount and the out-of-plane deformation amount. When only the in-plane deformation amount is acquired, the reference optical system 30 may be omitted.

  Further, the optical path change driving units 16 and 17 may be omitted. When the optical path change driving units 16 and 17 are omitted, the branched light guide optical system 12 is irradiated with two measurement beams 22A and 22B superimposed on the inspection target region so that the in-plane deformation amount is appropriately acquired. It may be arranged in advance. In this case, when acquiring the blood flow image, the control unit 50 may block one of the two measurement lights 22A and 22B, or the biological tissue without branching the measurement light emitted from the coherent light source 2 May be irradiated.

  In addition, an optical system may be arranged in advance so that one of the two measurement beams 22A and 22B is irradiated on the living tissue and the other is guided to the reference optical system 30. FIG. 6 is a diagram illustrating a part of an example of an optical system of a biological diagnosis apparatus when only one measurement light 22B is irradiated onto a biological tissue. In the optical system shown in FIG. 6, the measurement light emitted from the coherent light source 2 is branched into two measurement lights 22A and 22B by the branching optical element 11 which is a half mirror. The measurement light 22A reflected from the branching optical element 11 is reflected by the reference surface 31 of the reference optical system 30 and received by the light receiving element 40 as reference light. The measurement light 22 transmitted through the branch optical element 11 is irradiated to the fundus of the subject's eye E. The reflected light reflected by the fundus and reflected by the branching optical element 11 is received by the light receiving element 40 while interfering with the reference light. As described above, the configuration of the optical system can be changed as appropriate. In the example illustrated in FIG. 6, the control unit 50 may block the reference light guided to the light receiving element 40 or change the optical path of the reference light when acquiring the blood flow image. Good. Further, the control unit 50 may cause the branching optical element 11 to leave the optical path when acquiring a blood flow image.

DESCRIPTION OF SYMBOLS 1 Biodiagnosis apparatus 2 Coherent light source 10 Irradiation optical system 11 Branch optical element 12 Branch light guide optical system 16, 17 Optical path change drive part 22A, 22B Measurement light 30 Reference optical system 40 Light receiving element 50 Control part 61 Blood flow image 62 Deformation amount Distribution image 63 Duplicate image

Claims (6)

A biodiagnostic device,
A coherent light source that emits measurement light that is coherent light; and
An irradiation optical system for irradiating the examination target region of the living tissue with the measurement light emitted from the coherent light source;
A light receiving element that receives the reflected light of the measurement light reflected by the inspection target region;
A control unit that controls the biodiagnosis device;
With
The controller is
While obtaining a blood flow image showing a two-dimensional distribution of blood flow velocity in the examination region from the time change of the laser speckle in each of a plurality of images continuously photographed by the light receiving element,
From the phase information of the laser speckle in the image photographed by the light receiving element at the first time and the laser speckle in the image photographed by the light receiving element at the second time different from the first time, the inspection is performed. A biodiagnosis apparatus characterized by acquiring a deformation amount of the living tissue in a target region. The biodiagnosis device according to claim 1,
The irradiation optical system is
A branching optical element that branches the measurement light emitted from the coherent light source;
A branched light guide optical system that guides each of the plurality of measurement lights branched by the branch optical element toward the living tissue;
With
The controller is
When acquiring the deformation amount of the living tissue, each of the plurality of measurement beams branched by the branch optical element is irradiated on the same examination target region at each of the first time and the second time. A biodiagnosis apparatus characterized by acquiring an image. The biodiagnosis device according to claim 2,
An optical path change driving unit that changes at least one optical path of the branched measurement light by driving the branched light guiding optical system;
The controller is
When acquiring the deformation amount of the living tissue, by controlling the driving of the optical path changing drive unit, each of the plurality of measurement lights branched by the branch optical element is irradiated on the same examination target region. Let
When acquiring the blood flow image, by controlling the driving of the optical path changing drive unit, each of the plurality of measurement light beams branched by the branch optical element is not overlapped with each other. A biodiagnosis apparatus characterized by irradiating with a laser beam. The biodiagnosis device according to claim 1,
A branching optical element that branches the measurement light emitted from the coherent light source;
A branched light guide optical system for guiding at least one of a plurality of measurement lights branched by the branch optical element to the living tissue;
A reference optical system that guides the measurement light different from the measurement light guided to the living tissue among the plurality of measurement lights branched by the branch optical element as reference light to the light receiving element,
With
The controller is
When acquiring the deformation amount of the biological tissue, the reflected light of the measurement light irradiated on the biological tissue by the branched light guiding optical system and the reference optical system at each of the first time and the second time. A biodiagnosis device characterized by acquiring an image obtained by interference light with a guided reference light. The biodiagnosis device according to claim 4,
An optical path change driving unit that changes at least one optical path of the branched measurement light by driving the branched light guiding optical system;
The controller is
When acquiring the amount of deformation of the biological tissue, by controlling the driving of the optical path changing drive unit, one of a plurality of measurement lights branched by the branching optical element is irradiated to the examination target region and branched. Guiding other measurement light to the reference optical system,
When acquiring the blood flow image, by controlling the driving of the optical path changing drive unit, each of the plurality of measurement light beams branched by the branch optical element is not overlapped with each other. A biodiagnosis apparatus characterized by irradiating with a laser beam. The biodiagnosis device according to any one of claims 1 to 5,
The controller is
A deformation amount distribution image showing a two-dimensional distribution of the deformation amount of the living body in the inspection target region;
A biodiagnosis apparatus, wherein the deformation distribution image is displayed on a display unit so as to overlap or be aligned with the blood flow image acquired for the same region to be examined.

Images