JP2018157757A - Biological sample conveying device and biological sample conveyance method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a biological sample conveying device and a biological sample conveyance method by which a biological sample can be easily conveyed without damaging the sample and arranged on a desired place.SOLUTION: A biological sample conveying device 1 comprises: a dielectric layer 12 having an adsorbing surface 12a which adsorbs a biological sample; an electrode 14 provided on the opposite side to the adsorbing surface 12a of the dielectric layer 12; a grounding part 16 provided separately from the dielectric layer 12; and a voltage application mechanism 18 which applies voltage between the electrode 14 and the grounding part 16, in which the surface potential of the adsorbing surface 12a of the dielectric layer 12 is designed to change by applying voltage between the electrode 14 and the grounding part 16. Further, in a biological sample conveyance method, the biological sample is conveyed by using the biological sample conveying device 1, and is released at a desired place.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a biological sample transport apparatus and a biological sample transport method.

  In recent years, in the field of regenerative medicine, attempts have been made to enhance the therapeutic effect by placing a thick cell sheet obtained by culturing cells at high density in an affected area and engrafting it. As a method for producing an organized biological sample such as a cell sheet, for example, a temperature-responsive polymer that is hydrophobic at a culture temperature (for example, 37 ° C.) and hydrophilic at room temperature (20 to 25 ° C.) is used. A method for producing a biological sample using a culture vessel coated on the inner surface of the vessel is known (Patent Documents 1 and 2).

  In this method, since the temperature-responsive polymer on the surface of the container is hydrophobic and contracted during culture, the cells are adsorbed on the surface of the container and the organization thereof is promoted. In addition, when the temperature of the culture vessel is lowered to room temperature after culturing, the temperature-responsive polymer becomes hydrophilic and swelled, and water enters between the formed biological sample and the surface of the vessel, so that the biological sample becomes the surface of the vessel. Detaches intact. Since the biological sample obtained in this way is not stimulated by heat or light upon detachment, the original function of the cell is not lost.

  The biological sample desorbed from the surface of the container is transported from a culture container on a transporting member such as a non-woven fabric, and is attached to another biological sample or a transplant site. However, when a nonwoven fabric or the like is used, the detachment of the biological sample becomes a problem. After attaching a biological sample to another biological sample or transplantation site, if you attempt to remove the non-woven fabric before the biological sample is engrafted on the affected living tissue, the biological sample follows the non-woven fabric and another biological sample or transplant. It will peel off from the part. Furthermore, if the non-woven fabric or the like is left attached until the biological sample is engrafted in the transplanted biological tissue, the adhesion between the biological sample and the non-woven fabric becomes stronger, and the biological sample is damaged when the non-woven fabric is removed. Sometimes.

Japanese Patent No. 3441530 Japanese Patent No. 3641301

  An object of the present invention is to provide a biological sample transport apparatus and a biological sample transport method that can easily transport a biological sample without damaging it and place it at a target location.

The present invention has the following configuration.
[1] A dielectric layer having an adsorption surface for adsorbing a biological sample, an electrode provided on the opposite side of the dielectric layer from the adsorption surface, and a ground portion provided apart from the dielectric layer; An applied voltage mechanism for applying a voltage between the electrode and the ground part, and the surface potential of the adsorption surface is changed by applying a voltage between the electrode and the ground part. A biological sample transport device.
[2] The [1] A biological sample conveying method using the biological sample transporting device described in said higher than the potential of the electrode relative to the grounded portion by applying a voltage mechanism 0 (V) E ₁ ( V), and the transporting step of transporting the biological sample with the conductive fluid adhering to the suction surface, and the potential of the electrode with respect to the ground portion is lower than E ₁ (V) by the applied voltage mechanism A biological sample transport method, comprising: E ₂ (V), and a desorption step of desorbing the transported biological sample from the adsorption surface.

If the biological sample transport apparatus of the present invention is used, the biological sample can be easily transported without being damaged and placed at a target location.
According to the biological sample transport method of the present invention, the biological sample can be easily transported without being damaged and placed at a target location.

It is the schematic diagram which showed an example of the biological sample conveyance apparatus of this invention. It is the schematic diagram which showed a mode that a biological sample was conveyed using the biological sample conveyance apparatus of FIG. It is the graph which showed the relationship between the surface potential of the inner bottom face of a recessed part, and the protein adsorption rate Q in the protein adhesiveness test of an Example. It is the graph which showed the relationship between the stationary time and the protein adsorption rate Q in the surface potential -3V of the inner bottom face of the recessed part in the protein desorption test of an Example.

The following definitions of terms apply throughout this specification and the claims.
The “fluorinated polymer” means a polymer compound having a fluorine atom in the molecule.
The “unit” means a part derived from a monomer that exists in the polymer and constitutes the polymer. The unit derived from the monomer resulting from addition polymerization of a monomer having a carbon-carbon unsaturated double bond is a divalent unit generated by cleavage of the unsaturated double bond. Moreover, what unitally converted the structure of a unit after polymer formation is also called a unit. Hereinafter, in some cases, a unit derived from an individual monomer is referred to as a name obtained by adding “unit” to the monomer name.
“The fluoropolymer has an aliphatic ring in the main chain” means that at least one of the carbon atoms constituting the ring skeleton of the aliphatic ring is a carbon atom constituting the main chain of the fluoropolymer. Means that.

[Biological sample transport device]
The biological sample transport apparatus of the present invention is provided with a dielectric layer having an adsorption surface for adsorbing a biological sample, an electrode provided on the opposite side of the dielectric layer from the adsorption surface, and a distance from the dielectric layer. And an applied voltage mechanism for applying a voltage between the electrode and the earth part.
In the biological sample transport apparatus of the present invention, the surface potential of the adsorption surface of the dielectric layer is changed by applying a voltage between the electrode and the ground portion by the applied voltage mechanism. Since the surface potential of the adsorption surface of the dielectric layer changes, the adsorptivity of the biological sample to the adsorption surface changes, so that desorption of the biological sample can be controlled.

  In the biological sample transport apparatus of the present invention, the surface potential of the entire adsorption surface for adsorbing the biological sample can be uniformly controlled to a positive potential or a negative potential because the biological sample such as a cell sheet can be easily transported and desorbed. It is preferable that it is such.

FIG. 1 is a cross-sectional view showing an example of the biological sample transport apparatus of the present invention.
The biological sample transport apparatus 1 includes a container 10, a dielectric layer 12, an electrode 14, a ground unit 16, and a voltage application mechanism 18.
The accommodating body 10 is integrally formed so as to protrude from the electrode 14 side of the attracting portion 20 and the attracting portion 20 accommodating the dielectric layer 12 and the electrode 14, and the gripping portion accommodating the ground portion 16 and the applied voltage mechanism 18. 22.

The dielectric layer 12 has an adsorption surface 12 a that adsorbs a biological sample, and is provided so that the adsorption surface 12 a is exposed from the container 10. The electrode 14 is provided in the adsorption portion 20 on the side opposite to the adsorption surface 12 a of the dielectric layer 12. In this example, the dielectric layer 12 is provided on the flat electrode 14.
The ground portion 16 is provided in the gripping portion 22 so as to be separated from the dielectric layer 12 and not electrically connected to the dielectric layer 12. The applied voltage mechanism 18 is provided between the electrode 14 and the ground portion 16 in the grip portion 22 while being electrically connected to the electrode 14 and the ground portion 16. A voltage can be applied between the electrode 14 and the ground portion 16 by the applied voltage mechanism 18.
The surface potential of the attracting surface 12 a of the dielectric layer 12 is changed when a voltage is applied between the electrode 14 and the ground portion 16 by the applied voltage mechanism 18.

(Container)
The shape of the container is not particularly limited, and may be appropriately designed in consideration of transporting the biological sample in a state of being adsorbed on the adsorption surface where the adsorption layer is exposed. For example, as the biological sample transport apparatus 1, the container includes an adsorption unit that accommodates a dielectric layer and an electrode, a grounding unit and a voltage applying mechanism, and a gripping unit that is gripped by hand. Can be mentioned. The gripping part may be any shape that incorporates an applied voltage mechanism and a grounding part and can be gripped by hand. Examples thereof include a columnar shape.
As a material for forming the container, a known material that can be insulated from an electrode or an applied voltage mechanism so that electricity does not flow to the hand when touched by a hand can be used, and examples thereof include resins such as polyacetal and ABS resin. It is done.

(Dielectric layer)
The adsorption surface of the dielectric layer is a surface on which a biological sample is adsorbed during transportation.
The material for forming the dielectric layer is not particularly limited, and examples thereof include organic polymer materials such as a fluorine-containing polymer, polyethylene terephthalate, polyimide, polycarbonate, acrylic resin, parylene C, and the like. Coalescence is preferred.
The material for forming the dielectric layer may be one type or two or more types.

  The fluorine-containing polymer is not particularly limited, and examples thereof include a fluorine-containing polymer having a fluoroolefin unit, a fluorine-containing polymer having an aromatic ring, a fluorine-containing polymer having a fluoropolyether chain, and an aliphatic ring in the main chain. And fluorine-containing polymers, fluororubbers and the like.

Examples of the fluoropolymer having a fluoroolefin unit include polytetrafluoroethylene (PTFE), tetrafluoroethylene-perfluoro (alkyl vinyl ether) copolymer (PFA), and the like.
Examples of the fluorine-containing polymer having an aromatic ring include fluorine-containing aromatic compounds (perfluoro (1,3,5-triphenylbenzene) and the like) and phenolic compounds (1,3,5-trihydroxybenzene and the like). And a polymer obtained by reacting a crosslinkable functional group-containing aromatic compound (such as pentafluorostyrene) in the presence of a dehydrohalogenating agent (such as potassium carbonate).
Examples of the fluoropolymer having a fluoropolyether chain include OPTOOL DSX (registered trademark, manufactured by Daikin Industries), KY-100 series (manufactured by Shin-Etsu Chemical Co., Ltd.), and the like.
Examples of the fluorine-containing polymer having an aliphatic ring in the main chain include CYTOP (registered trademark, manufactured by Asahi Glass Co., Ltd.), Teflon (registered trademark) AF (manufactured by DuPont), and the like.
Examples of the fluororubber include vinylidene fluoride-based fluororubbers mainly composed of vinylidene fluoride (VDF), propylene-tetrafluoroethylene-based fluororubber, tetrafluoroethylene-perfluoroalkylvinylether-based fluororubber, and the like.
As a fluoropolymer, 1 type may be used independently and 2 or more types may be used in combination.

  As a material for forming the dielectric layer, one or more metal oxides selected from the group consisting of silica, alumina, ceria, titania and zirconia may be used.

  The thickness of the dielectric layer is preferably 0.1 to 10 μm, and more preferably 0.5 to 1 μm. If the thickness of the dielectric layer is equal to or greater than the lower limit, dielectric breakdown is less likely to occur within a practical working voltage. If the thickness of the dielectric layer is not more than the upper limit value, the dielectric layer can be efficiently polarized by applying a voltage.

(electrode)
The material for forming the electrode may be a conductive material, for example, indium tin oxide (ITO); metal such as Pt, Au, Ni, Al, etc .; conductivity such as SnO ₂ , In ₂ O ₃ , RuO _2, etc. Metal oxides having: semiconductors such as Ge, Si and GaAs; carbon-based materials such as graphite, glassy carbon and diamond; conductive polymers such as polyacetylene, polypyrrole, polyaniline and polythiophene.
The shape of the electrode is not particularly limited, and examples thereof include a flat plate shape and a comb shape.
The number of electrodes is not limited to one and may be two or more.

(Earth part)
The grounding part is not particularly limited as long as it can be grounded while being built in the apparatus, and a known part can be adopted. Specific examples of the ground portion include, for example, a body provided on the grip portion so as to come into contact with the human body, a metal plate such as aluminum, and the like. In this case, the metal plate is grounded through the human body.

(Applied voltage mechanism)
As the applied voltage mechanism, any mechanism can be used as long as an arbitrary voltage can be applied between the electrode and the ground portion, and a known mechanism can be adopted.
As the applied voltage mechanism, a mechanism including a switch for switching ON / OFF of voltage application is preferable. Further, as a voltage application mechanism, a switch for switching a positive voltage output unit, a negative voltage output unit, and whether to apply a voltage between the positive voltage output unit or the negative voltage output unit between the electrode and the ground unit The thing provided with is preferable. Furthermore, as an applied voltage mechanism, what is equipped with the voltage adjustment mechanism which can adjust the voltage applied between an electrode and an earth | ground part is preferable.

(Production method)
The manufacturing method of the biological sample transport apparatus of the present invention is not particularly limited, and examples thereof include the following methods.
A coating solution in which a fluorine-containing polymer or the like is dissolved in a solvent is applied on a flat electrode and dried by baking or the like to form a dielectric layer. An electrode and a dielectric layer are accommodated in a container having a built-in voltage application mechanism and a ground portion that are electrically connected to each other so that the adsorption surface of the dielectric layer is exposed, and the electrode and the voltage application mechanism are electrically connected. A biological sample transport apparatus is obtained by connection.

  The coating method for the coating solution is not particularly limited, and examples thereof include spin coating, dip coating, cast coating, spray coating, die coating, scan coating, brush coating, and potting.

[Biological sample transport method]
The biological sample transport method of the present invention is a method of transporting a biological sample using the biological sample transport device of the present invention. The biological sample transport method of the present invention includes the following transport process and desorption process.
Transport process: Using a biological sample transport device, the potential of the electrode with respect to the grounding portion is set to E ₁ (V) higher than 0 (V) by the applied voltage mechanism, and the biological sample with the conductive fluid attached is placed on the dielectric layer Adsorbed to the adsorption surface and transported.
Desorption step: The potential of the electrode with respect to the ground portion is set to E ₂ (V) lower than E ₁ (V) by the applied voltage mechanism, and the transported biological sample is desorbed from the adsorption surface.

(Conveying process)
For example, in a biological sample transport device, the potential of the electrode with respect to the ground portion is set to E ₁ (V) higher than 0 (V) by the applied voltage mechanism, and the living surface in which the conductive fluid is attached to the adsorption surface of the dielectric layer Contact the sample. Since the potential of the electrode with respect to the ground portion is E ₁ (V), the surface potential of the attracting surface of the dielectric layer becomes a positive potential, and the attracting surface is positively charged. Since a cell is normally negatively charged, a biological sample is adsorbed by an electrostatic interaction on a positively charged adsorption surface. In this manner, the biological sample is transported to a target location in a state where the biological sample is adsorbed on the adsorption surface of the dielectric layer.

For example, when the biological sample 110 obtained by cell culture is transported using the biological sample transport apparatus 1 illustrated in FIG. 1, the culture solution is removed from the culture vessel 100 prior to the state of FIG. The liquid medium is attached to 110. Next, as shown in FIG. 2A, in the biological sample transport apparatus 1, the potential of the electrode 14 with respect to the ground portion 16 is set to E ₁ (V) higher than 0 (V) by the applied voltage mechanism 18, and the dielectric layer The 12 adsorption surfaces 12a are brought into contact with the biological sample 110 with the liquid medium attached thereto. As shown in FIG. 2B, since the biological sample 110 is adsorbed to the adsorption surface 12a of the dielectric layer 12 by electrostatic interaction, the biological sample 110 is transported to a target location in that state.

The potential E ₁ (V) of the electrode with respect to the ground part in the culturing step can be appropriately determined depending on the cell type within a range higher than 0 (V).

The biological sample is not particularly limited, and examples thereof include proteins, glycoproteins, and cells.
Examples of cells include gingival fibroblasts, periodontal ligament cells, epidermal cells, fibroblasts, liver parenchymal cells, liver non-parenchymal cells (endothelial cells, Kupffer cells, stellate cells, etc.), osteoblasts, epithelial cells, Examples include chondrocytes, nerve cells, muscle cells, mesenchymal stem cells, islet cells, macrophages, various tumor cells and functional cells. The cell may be an artificial cell.
A cell sheet is preferred as the biological sample.

  The conductive fluid is not particularly limited as long as it does not adversely affect the biological sample, and examples thereof include a liquid medium for cell culture, phosphate buffer (D-PBS), and physiological saline.

(Desorption process)
After the transfer, the potential of the electrode with respect to the ground portion is set to E ₂ (V) lower than E ₁ (V) by the applied voltage mechanism at the target location, and the biological sample is desorbed from the adsorption surface. For example, in the state where the biological sample adsorbed on the adsorption surface of the dielectric layer is in contact with the affected part of the patient, the potential of the electrode with respect to the ground part is set to E ₂ (V) lower than E ₁ (V), and the biological sample Is detached from the suction surface and placed on the affected area.

When the biological sample 110 is transferred using the biological sample transfer apparatus 1 illustrated in FIG. 1, for example, as shown in FIG. 2C, the biological sample 110 adsorbed on the adsorption surface 12 a of the dielectric layer 12. Is in contact with the affected part 200 of the patient, and the potential of the electrode 14 with respect to the ground part 16 is set to E ₂ (V) lower than E ₁ (V). Then, as shown in FIG. 2D, the biological sample 110 is detached from the adsorption surface 12 a of the dielectric layer 12 by placing the biological sample transport apparatus 1 away from the affected part 200, and is placed on the affected part 200.

In the desorption process, it is preferable that the potential E ₂ (V) of the electrode with respect to the ground portion is set to a negative potential by the applied voltage mechanism. As a result, the surface potential of the attracting surface of the dielectric layer becomes negative and the attracting surface is negatively charged. Therefore, the biological sample is more easily detached from the adsorption surface due to electrostatic repulsion between the adsorption surface of the dielectric layer and the biological sample. In this case, the potential of the electrode with respect to the ground portion can be appropriately determined according to the cell type within a range where E ₂ (V) is lower than 0 (V).

In the desorption process, when the biological sample is placed in a place where grounding is possible, the application of the voltage by the applied voltage mechanism is turned off after the biological sample is placed in the place, and the potential E ₂ of the electrode with respect to the ground portion. (V) may be set to 0 (V). As a result, the dielectric layer and the electrode are grounded via the biological sample that is in contact with a place where grounding is possible, and the electrostatic interaction between the adsorption surface and the biological sample is eliminated. Detach.
For example, the biological sample is detached from the adsorption surface even if the voltage applied by the applied voltage mechanism is simply turned off while the biological sample conveyed while adsorbed on the adsorption surface of the dielectric layer is in contact with the affected part of the patient. Placed on the affected area.

As described above, in the present invention, the surface potential of the adsorption surface of the dielectric layer is adjusted, and the surface properties of the adsorption surface are changed to change the adhesiveness of the cells. Can control cell detachment. Therefore, the biological sample can be transported with a low load without applying a stimulus such as heat, light or vibration, and the biological sample can be easily detached from the adsorption surface as compared with the case of using a nonwoven fabric or the like. As described above, in the present invention, the biological sample can be transported and placed at a target location while suppressing the loss of the original function of the biological sample.
As an application of the present invention, a biological sample can be transported and placed at a target location without impairing the original function of the cell. It is effective in that it can be engrafted in a living tissue efficiently.

EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited by the following description.
[Example 1]
(Preparation of coating solution)
Using CYTOP (registered trademark, manufactured by Asahi Glass Co., Ltd.), a coating solution was prepared by dissolving in CT-solv100E (manufactured by Asahi Glass Co., Ltd.) so that the concentration thereof was 0.9% by mass.
(Production of evaluation device)
An ITO film having a thickness of 150 nm and a sheet resistance value of 10 Ω / cm ² was formed as an electrode on a glass substrate. Further, the coating solution was applied onto the electrode by spin coating to form a dielectric layer having a thickness of 0.5 μm. In spin coating, the glass substrate was spun at 500 rpm for 10 seconds and then spun at 1000 rpm for 20 seconds.
Next, a polystyrene petri dish having a through hole having a diameter of 35 mm formed on the bottom surface portion is adhered to the dielectric layer using an adhesive, and an evaluation device including a recess having an inner bottom surface formed of the dielectric layer is manufactured. did.

[Protein adhesion test]
The following measurements were made with reference to Advances in Polymer Science 57 Polymers in Medicine. (Western book) and Surface modification of polymers for medical applications. Biomaterials 1994. Vol.15 No.10 725-736.
(1) Preparation of color developing solution and protein solution As a color developing solution, 50 mL of peroxidase coloring solution (3,3 ′, 5,5′-tetramethylbenzidine (TMBZ), manufactured by KPL) and TMB Peroxidase Substrate (manufactured by KPL) ) Was mixed with 50 mL.
As a protein solution, a protein (POD-goat anti mouse IgG, Biorad) diluted 16,000 times with a phosphate buffer solution (D-PBS, Sigma) was used.
(2) Protein adsorption 3 mL of the protein solution is dispensed into the recess of the evaluation device, the counter electrode is arranged, a voltage of 3 V is applied to the evaluation device, and the electrode potential of the evaluation device is set to 3 V at room temperature. The protein was adsorbed by standing for 1 hour. Separately from this, the same operation was performed except that the electrode potential of the evaluation device was set to -3V. In addition, a voltage was not applied to the evaluation device and the electrode potential of the evaluation device was set to 0 V, and the device was left at room temperature for 1 hour in the same manner as described above. In these, it can be considered that the surface potential of the inner bottom surface of the recess of the evaluation device is equivalent to the electrode potential.
As a blank, 2 μL of the protein solution was dispensed into 3 wells in a 96-well microplate per well.
(3) Well Washing Phosphate buffer solution (D-PBS, manufactured by Sigma) containing 0.05% by mass of a surfactant (Tween 20, manufactured by Wako Pure Chemical Industries, Ltd.) in the recess of the evaluation device on which the protein is adsorbed ) 4 times (using 16 mL).
(4) Coloring solution dispensing 3 mL of the coloring solution was dispensed into the recesses of the evaluation device that had been washed, and a coloring reaction was performed for 7 minutes. The color reaction was stopped by adding 1.5 mL of 2N sulfuric acid.
For the blank, 100 μL of the coloring solution was dispensed into 3 wells of a 96-well microplate for 7 minutes, and then the coloring reaction was performed by adding 50 μL of 2N sulfuric acid to each well. Stopped.
(5) Preparation for absorbance measurement 150 μL of the liquid was taken from the recess of the evaluation device and transferred to a 96-well microplate.
(6) Absorbance measurement and protein adsorption rate Q
Absorbance was measured at 450 nm using MTP-810Lab (Corona Electric Co., Ltd.). Mean absorbance of blank (N = 3) was _{A 0.} The inner bottom surface of the surface potential of the recess (electrode potential of the evaluation device) 3V, -3 V, for each of those and 0V, the absorbance of the liquid is moved from the evaluation device to a 96-well microplate was A _1.
The protein adsorption rate Q was determined by the following equation.
Q = A ₁ / {A ₀ × (100 / amount of dispensed blank protein solution)} × 100
= A ₁ / {A ₀ × (100/2 μL)} × 100 [%]
The results are shown in FIG.

  As shown in FIG. 3, in the evaluation device of Example 1, the protein adsorption rate Q increased in the order of the surface potential of the inner bottom surface of the recess (electrode potential of the evaluation device) of −3V, 0V, and 3V. This result shows that the adsorptivity of the biological sample to the adsorption surface can be controlled by controlling the surface potential of the adsorption surface of the dielectric layer.

[Protein desorption test]
(1) Protein adsorption 3 mL of the protein solution is dispensed into the concave portion of the evaluation device, a counter electrode is placed, a voltage of 3 V is applied to the evaluation device, and the surface potential of the inner bottom surface of the concave portion (device for evaluation) The electrode potential was set at 3 V and allowed to stand at room temperature for 1 hour to adsorb proteins.
As a blank, 2 μL of the protein solution was dispensed per well into 3 wells in a 96-well microplate.
(2) Protein Desorption A voltage of −3 V was applied to the evaluation device on which the protein was adsorbed, and the surface potential of the inner bottom surface of the recess (the electrode potential of the evaluation device) was set to −3 V, and left at room temperature for 1 minute. Next, the recess was washed four times with 4 mL of a phosphate buffer solution (D-PBS, manufactured by Sigma) containing 0.05% by mass of a surfactant (Tween 20, manufactured by Wako Pure Chemical Industries, Ltd.).
Moreover, it carried out similarly about what set the standing time in the state which set the surface potential of the inner bottom face of a recessed part to -3V as 0 minute, 5 minutes, and 10 minutes. Note that the standing time of 0 minutes means that cleaning was performed with the surface potential of the inner bottom surface of the recess (electrode potential of the evaluation device) set to 0V.
(3) Coloring solution dispensing, absorbance measurement preparation, absorbance measurement, and protein adsorption rate Q
In the same manner as in (4) to (6) of [Protein Adhesion Test], the protein adsorption rate Q was measured for each of the standing times of 0 minutes, 1 minute, 5 minutes, and 10 minutes.
The results are shown in FIG.

  As shown in FIG. 4, in the evaluation device of Example 1, after allowing the protein to be adsorbed, the standing time with the surface potential of the inner bottom surface of the recess (electrode potential of the evaluation device) being −3 V was 10 minutes. As a result, the protein adsorption rate Q was significantly lower than when the standing time was 0 minutes. This result shows that by controlling the surface potential of the adsorption surface of the dielectric layer, it is possible to desorb the biological sample at the target location after adsorbing and transporting the biological sample to the adsorption surface. Is.

DESCRIPTION OF SYMBOLS 1 Biological sample conveyance apparatus 10 Container 12 Dielectric layer 12a Adsorption surface 14 Electrode 16 Grounding part 18 Applied voltage mechanism 20 Adsorption part 22 Gripping part

Claims (2)

A dielectric layer having an adsorption surface for adsorbing a biological sample, an electrode provided on the opposite side of the dielectric layer from the adsorption surface, a ground portion provided apart from the dielectric layer, and the electrode A voltage applying mechanism for applying a voltage between the ground part, and
The biological sample transport apparatus, wherein the surface potential of the adsorption surface is changed by applying a voltage between the electrode and the ground part. A biological sample transport method using the biological sample transport device according to claim 1,
A transporting step of transporting the biological sample having a conductive fluid attached thereto by adsorbing to the adsorption surface by setting the potential of the electrode with respect to the ground portion to E ₁ (V) higher than 0 (V) by the applied voltage mechanism. When,
A desorption step of setting the potential of the electrode with respect to the ground portion to E ₂ (V) lower than E ₁ (V) by the applied voltage mechanism, and desorbing the transported biological sample from the adsorption surface. Biological sample transport method.

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