JP2018012085A - Connection structure between pipette tip and nozzle to be fitted to the pipette tip - Google Patents

Connection structure between pipette tip and nozzle to be fitted to the pipette tip Download PDF

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JP2018012085A
JP2018012085A JP2016144359A JP2016144359A JP2018012085A JP 2018012085 A JP2018012085 A JP 2018012085A JP 2016144359 A JP2016144359 A JP 2016144359A JP 2016144359 A JP2016144359 A JP 2016144359A JP 2018012085 A JP2018012085 A JP 2018012085A
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pipette tip
pipette
nozzle
tip
inner diameter
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JP6780338B2 (en
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孝 小嶋
Takashi Kojima
小嶋　　孝
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Pentel Co Ltd
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Pentel Co Ltd
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Abstract

PROBLEM TO BE SOLVED: To solve such a problem that, in consideration that a distance from a tip of a pipette cylinder to a pipette tip gets to constant, further, a circumferential seal is provided on an inner diameter part of the pipette tip and sealability is secured by subjecting the pipette tip to frictional conforming to a lateral wall (outer circumferential surface) of the pipette cylinder, it is required that, when the pipette tip is fitted to the pipette, the pipette is perpendicularly and coaxially set with respect to the pipette tip and, as the result, the appropriate fitting state cannot be maintained without using a fitting tool or the like.SOLUTION: In a connection structure between a pipette tip and a nozzle to be fitted to the pipette tip, a taper angle of a back side inner diameter part is formed to be larger than a taper angle of outer diameter of a nozzle part of the nozzle, and an air-tight part on the circumference of a circle is provided on the tip side of the nozzle part and a circumferential rib is provided on the back side inner diameter part of the pipette tip.SELECTED DRAWING: Figure 1

Description

本発明は、ピペットや分注器、検査機器などを用いて実験や検査などを行う際、使用されるピペットチップ本体とフィルターからなるピペットチップに関する。   The present invention relates to a pipette tip composed of a pipette tip body and a filter that are used when performing experiments or inspections using a pipette, a dispenser, an inspection device, or the like.

実験や検査などで使用されるピペットチップは、使用するピペットや分注器、検査機器など、その用途によって１０〜３００μＬまたは、それ以上の容量と大小さまざまなサイズが市販されている。多くは使用後、廃棄されてしまう使い捨てのピペットチップで、その材質はプラスチック製が大半を占めている。   Pipette tips used in experiments and inspections are commercially available in various sizes of 10 to 300 μL or more depending on their use, such as pipettes, dispensers, and inspection devices. Most are disposable pipette tips that are discarded after use, and the material is mostly plastic.

ピペットなどのノズル部はテーパ形状で構成されており、ピペットチップの内径面もテーパ形状で構成されている。しかし、互いのテーパ角度は異なり、ピペットチップの種類によってはノズル部のテーパ角度より大きいものがある。使用時、そのテーパ同士を圧入し、装着するが、エアタイト部（密閉）は円周上（リング状）に1ヶ所で、且つ、密閉されていない箇所には隙間が生じるため、そのガタ分、ピペットとピペットチッブの軸心がズレやすく、且つ、横方向の力が働くと外れやすく、ひいては、密閉性に影響が出てきてしまう恐れがあった。   A nozzle portion such as a pipette has a tapered shape, and an inner diameter surface of the pipette tip also has a tapered shape. However, the taper angles are different from each other, and some types of pipette tips are larger than the taper angle of the nozzle portion. During use, the tapers are press-fitted together and attached, but the air tight part (sealed) is one place on the circumference (ring shape), and there is a gap in the part that is not sealed. The axial center of the pipette and the pipette tip is easily displaced, and when the force in the lateral direction is applied, it is likely to come off, which may affect the sealing performance.

仮にテーパ角度が同一の場合、エアタイト部（密閉）がテーパ部全体に及ぶため、円周上（リング状）のエアタイト部（密閉）が形成しにくくなり、ややもすると、Ｃリング状のエアタイト部（密閉）が形成される恐れが出てくる。この場合、本来のピペットに機能である定量の注入を阻害することもある。   If the taper angle is the same, the air tight part (sealed) extends over the entire taper part, making it difficult to form an air tight part (sealed) on the circumference (ring shape). There is a risk that (sealed) will be formed. In this case, a certain amount of injection, which is a function of the original pipette, may be hindered.

特開昭６０−１３７４４３号公報JP 60-137443 A

この従来技術は、ピペットチップの内径部に停止部である着座用の棚（肩）を設け、ピペットを前記ピペットチップへ装着する際、ピペットの先端（ノズル）が前記着座用の棚（肩）と当接する。これにより、ピペットの筒の先端からピペットチップ先端の距離が一定となる。更に、前記ピペットチップの内径部には円周シールが設けてあり、前記ピペットの筒の側壁（外周面）に摩擦適合されることにより、密閉性を確保している。
しかし、ピペットにピペットチップを装着する際、ピペットチップに対してピペットを真っ直ぐに、且つ、同軸心に設定する必要があり、つまり、装着用治具などを用いなければ適切な装着状態を維持できない課題があった。 In this prior art, a seating shelf (shoulder), which is a stop, is provided on the inner diameter of the pipette tip, and when the pipette is mounted on the pipette tip, the tip (nozzle) of the pipette is the seating shelf (shoulder). Abut. As a result, the distance from the tip of the pipette tube to the tip of the pipette tip is constant. Further, a circumferential seal is provided on the inner diameter portion of the pipette tip, and the sealability is ensured by being frictionally fitted to the side wall (outer peripheral surface) of the pipette pipe.
However, when mounting the pipette tip on the pipette, it is necessary to set the pipette straight and coaxially with respect to the pipette tip, that is, an appropriate mounting state cannot be maintained unless a mounting jig or the like is used. There was a problem.

また、仮に治具を使用せず、手でピペットにピペットチップを装着した場合、装着前はテーパ分、径方向に隙間が生じるため、つまり、お互いの軸心がズレた状態で、ピペットの筒の側壁（外周面）がピペットチップの内径面と接触しながら挿入され、更に、先端（ノズル）の角部がピペットチップの内径部に設けた円周シールと接触して傷つけてしまい、密閉性を損なう恐れがあった。
装着用治具の定期的なメンテナンスなども作業に加わるため、実験や検査以外も注意を図り、気を使わなければならなかった。 Also, if the pipette tip is attached to the pipette by hand without using a jig, there will be a gap in the radial direction due to the taper before the attachment, that is, the pipette tube will be out of alignment with each other. The side wall (outer peripheral surface) of the pipette is inserted while being in contact with the inner diameter surface of the pipette tip, and the corner portion of the tip (nozzle) is in contact with the circumferential seal provided on the inner diameter portion of the pipette tip and is damaged. There was a risk of damage.
Since regular maintenance of the mounting jig was also added to the work, we had to be careful and careful other than experiments and inspections.

本発明は、ピペットチップとそのピペットチップに装着されるノズルとの連結構造であって、そのノズルのノズル部の外径のテーパ角度より、前記ピペットチップの後方内径部のテーパ角度を大きく形成すると共に、前記ノズル部の先端側に円周上のエアタイト部を設け、且つ、前記ピペットチップの後方の内径部に円周リブを設けたことを要旨とする。   The present invention is a connection structure between a pipette tip and a nozzle attached to the pipette tip, wherein the taper angle of the rear inner diameter portion of the pipette tip is larger than the taper angle of the outer diameter of the nozzle portion of the nozzle. In addition, the gist of the invention is that a circumferential air tight portion is provided on the tip side of the nozzle portion, and a circumferential rib is provided on the inner diameter portion of the rear of the pipette tip.

本発明は、ピペットチップとそのピペットチップに装着されるノズルとの連結構造であって、そのノズルのノズル部の外径のテーパ角度より、前記ピペットチップの後方内径部のテーパ角度を大きく形成すると共に、前記ノズル部の先端側に円周上のエアタイト部を設け、且つ、前記ピペットチップの後方の内径部に円周リブを設けたことにより、前記ノズル部のテーパ部とピペットチップのテーパ部のエアタイト部（密閉）とノズル部のテーパ部と円周リングと当接され、２ヶ所で圧入固定及び当接されているので、横方向の力が加わっても曲がりにくく、更に、前記ノズルと前記ピペットチップの装着時のガタがなく、且つ、同軸心となるため、前記ピペットチップに試薬やさまざまな液体などを定量注入後、プレパラートや試験管などの容器へ的確に、且つ、正確に分注可能とするピペットチップを提供することができる。   The present invention is a connection structure between a pipette tip and a nozzle attached to the pipette tip, wherein the taper angle of the rear inner diameter portion of the pipette tip is larger than the taper angle of the outer diameter of the nozzle portion of the nozzle. In addition, a taper portion of the nozzle portion and a taper portion of the pipette tip are provided by providing a circumferential air tight portion on the tip side of the nozzle portion and providing a circumferential rib on the inner diameter portion of the rear of the pipette tip. Since the air tight part (sealed), the taper part of the nozzle part and the circumferential ring are in contact with each other and are press-fitted, fixed and in contact with each other at two places, it is difficult to bend even when a lateral force is applied. Since there is no backlash when the pipette tip is mounted and it is coaxial, a reagent, various liquids, etc. are quantitatively injected into the pipette tip, and a preparation, test tube, etc. Precisely to the container, and it can provide a pipette tip to accurately can dispense.

本実施例の組立完了後の斜視図Perspective view after assembly of this embodiment is completed 図１の正面図Front view of FIG. 図２の縦断面図2 is a longitudinal sectional view 図２の下面図Bottom view of FIG. 本実施例の分解斜視図Exploded perspective view of this embodiment ピペットチップ本体２の正面図Front view of pipette tip body 2 図６の縦断面図6 is a longitudinal sectional view of FIG. フィルター３の正面図Front view of filter 3 図８の断面図Sectional view of FIG. 本実施例のノズル部４ａの装着前の正面図Front view before mounting of nozzle part 4a of the present embodiment 図１０の縦断面図10 is a longitudinal sectional view of FIG. 本実施例のノズル４ａの装着後の正面図Front view after mounting the nozzle 4a of the present embodiment 図１２の縦断面図12 is a longitudinal sectional view of FIG. 図１３の拡大図（Ａ部）Enlarged view of Fig. 13 (Part A)

本発明を実施するための形態を図面に基づいて説明する。図１乃至図１４は、本発明における実施例である。
ピペットチップ１は、ピペットチップ本体２とフィルター３で構成されている。前記ピペットチップ１は外面形状が円錐筒形状であると共に、内面形状も円錐形状を成している。尚、狭く尖っている方を前方、広い方を後方とする。前記ピペットチップ本体２は外面形状が円錐筒形状でああると共に、内面形状も円錐形状を成している。尚、狭く尖っている方を前方、広い方を後方とする。そのピペットチップ本体２は、プラスチック製の射出成形品である。
前記フィルター３は円柱形状であり、前後の方向性はなく、且つ、撥水性を有する樹脂製の繊維集束体であり、更に、通気性を有している。
前記フィルター３は前記ピペットチップ本体２の後方開口部２ａから挿入され、前記ピペットチップ本体２の中間部に形成されている段部２ｂと前記フィルター３の端面３ａが当接し、これ以上前方へ行かないようになっている。また、前記フィルター３の外径部３ｂは、前記ピペットチップ２の内径部２ｃより大きく形成されている。これにより、フィルター３はピペットチップ２内で圧入固定された状態にある。この圧入状態により、フィルター３は後方へ移動することもなく、また、後方開口部２ａから抜け落ちることもない。尚、この圧入力は輸送による振動や使用時に外れない程度の力であれば良い。また、フィルター３の気孔率は２０〜８０％の範囲で使用されるが、使用する液体（粘度）や異物の大きさにより変わる。
前記ピペットチップ本体２の外観の後方にたてリブ２ｄが軸心に対して円周上に外径面に凸形状で複数、均等に配置されている。前記ピペットチップ本体２はプラスチック製の射出成形品なので、前記内径部２ｃにヒケの発生を抑えるため、たてリブ２ｄのリブ巾Ｔに対して肉厚Ｕを２倍以上にしている。前記たてリブ２ｄはノズル装着時に掛かる荷重に対する受けや転がり防止など、様々な目的で設けられている。
ピペットチップ本体２はプラスチック製の射出成形品であり、本実施例の材質はＰＰ樹脂（ポリプロピレン）であるが、用途に合わせて、ＰＥ樹脂（ポリエチレン）、ＰＣ樹脂（ポリカーボネート）、ＰＳ樹脂（ポリスチレン）、ＰＭＭＡ樹脂（アクリル）、ＰＥＴ樹脂（ポリエチレンテレフタレート）でも良い。また、透明色や半透明色、着色など、適宜選択可能である。フィルター３は、本実施例では撥水性を有する樹脂製の繊維集束体であるが、その材質は用途に合わせて、ポリエステル繊維、アクリル繊維、ナイロン繊維、ポリプロピレン繊維など、適宜選択可能であり、その他の多孔質体（ウレタン、スポンジ、焼結体など）でも良く、その材質も樹脂、金属、セラミックや活性炭など通気性を有しているものなら良く、または形状も円柱形状以外に、用途に合わせて、円錐形状、球形状、楕円形状など、適宜選択可能である。また、上記、それぞれ組み合わせても良い。
ピペットチップ本体２の後方のテーパ内径部２ｅの中間部に形成されている円周溝２ｆは、射出成形の時、離型させるために必要な溝であり、前記テーパ内径部２ｄは１つのテーパ角度で形成されている。
ピペット本体２に液体などを滴下するピペット４は一般のマイクロピペットと仕様は同じく、ダイヤルで吸い込み量を設定し、一定量吸引し、吐出するようになっている。
さらに、前記ピペットチップ本体２のテーパ内径部２ｅのテーパ角度αは、前記ピペット４のノズル部４ａのテーパ外径部４ｂのテーパ角度βより、若干大きく、且つ、テーパ角度の差は３°以下となっている（α＞β，α−β≦３°）。 DESCRIPTION OF EMBODIMENTS Embodiments for carrying out the present invention will be described with reference to the drawings. 1 to 14 show an embodiment of the present invention.
The pipette tip 1 includes a pipette tip body 2 and a filter 3. The pipette tip 1 has a conical cylindrical shape on the outer surface and a conical shape on the inner surface. Note that the narrow and sharp one is the front and the wide one is the rear. The pipette tip body 2 has a conical cylinder shape on the outer surface and a conical shape on the inner surface. Note that the narrow and sharp one is the front and the wide one is the rear. The pipette tip body 2 is a plastic injection molded product.
The filter 3 has a cylindrical shape, has no front-rear directionality, is a resin fiber bundle having water repellency, and has air permeability.
The filter 3 is inserted from the rear opening 2a of the pipette tip body 2, and the step 2b formed in the middle portion of the pipette tip body 2 and the end surface 3a of the filter 3 come into contact with each other so that the filter 3 moves forward. There is no such thing. Further, the outer diameter portion 3 b of the filter 3 is formed larger than the inner diameter portion 2 c of the pipette tip 2. As a result, the filter 3 is press-fitted and fixed in the pipette tip 2. Due to this press-fitted state, the filter 3 does not move rearward and does not fall out of the rear opening 2a. It should be noted that this pressure input may be a force that does not deviate during transportation or during use. Moreover, although the porosity of the filter 3 is used in the range of 20 to 80%, it varies depending on the liquid (viscosity) used and the size of foreign matter.
A plurality of vertical ribs 2d are arranged on the outer circumference of the pipe center on the circumference with respect to the axial center. Since the pipette tip body 2 is a plastic injection-molded product, the wall thickness U is set to be twice or more the rib width T of the vertical rib 2d in order to suppress the occurrence of sink marks in the inner diameter portion 2c. The vertical rib 2d is provided for various purposes such as receiving a load applied when the nozzle is mounted and preventing rolling.
The pipette tip body 2 is an injection-molded product made of plastic, and the material of this embodiment is PP resin (polypropylene). However, PE resin (polyethylene), PC resin (polycarbonate), PS resin (polystyrene) are used depending on the application. ), PMMA resin (acrylic), and PET resin (polyethylene terephthalate). Further, a transparent color, a translucent color, a coloring, or the like can be selected as appropriate. The filter 3 is a resin fiber bundle having water repellency in this embodiment, but the material can be appropriately selected according to the use, such as polyester fiber, acrylic fiber, nylon fiber, polypropylene fiber, etc. Porous material (urethane, sponge, sintered body, etc.) may be used as long as the material is breathable, such as resin, metal, ceramic, activated carbon, etc. Thus, a conical shape, a spherical shape, an elliptical shape, or the like can be selected as appropriate. In addition, the above may be combined.
A circumferential groove 2f formed in an intermediate portion of the taper inner diameter portion 2e at the rear of the pipette tip body 2 is a groove necessary for releasing during injection molding, and the taper inner diameter portion 2d has one taper. It is formed at an angle.
The pipette 4 for dropping liquid or the like on the pipette body 2 has the same specifications as a general micropipette, and the suction amount is set with a dial, and a fixed amount is sucked and discharged.
Further, the taper angle α of the taper inner diameter portion 2e of the pipette tip body 2 is slightly larger than the taper angle β of the taper outer diameter portion 4b of the nozzle portion 4a of the pipette 4, and the difference in taper angle is 3 ° or less. (Α> β, α−β ≦ 3 °).

次に使用方法について説明する。前記ピペット４のノズル部４ａが後方から前方へ前進して前記ピペットチップ１への装着を開始する。この時、前記ピペットチップ本体２のテーパ内径部２ｅのテーパ角度αは、前記ノズル部４ａのテーパ外径部４ｂのテーパ角度βより、若干大きく形成されているため、前記ノズル部４ａと前記ピペットチップ１の軸心がズレても、前記テーパ内径部２ｅへ前記テーパ外径部４ｂの前方に配置する角部４ｃが接触し始め案内され、テーパ同士に沿って傷つかずに挿入され、且つ、軸心が少しずつ合わさる。更に、前記ノズル４ａを前進させると前記テーパ内径部２ｅと前記テーパ外径部４ｂの前方テーパ外径部４ｄが圧入固定され、且つ、前記テーパ内径部２ｅと前記前方テーパ外径部４ｄが軸心に対して円周上（リング状）に、且つ、帯状にエアタイト部（密閉）２ｇが設けられる。即ち、テーパー内径部２ｅがエアタイト部（密閉）２ｇとなる。使用時、前記エアタイト部（密閉）２ｇから空気が流入出して、前記ピペット４の機能（定量の注入、注出）を阻害することはない。それと同時に、前記テーパ内径部２ｅの後方に配置している円周リブ２ｈと前記テーパ外径部４ｂの後方テーパ外径部４ｅが軸心に対して円周上（リング状）に、且つ、帯状に当接部２ｉが軽い圧入で嵌装されている。
これにより、前記ピペットチップ１と前記ノズル部４ａは２ヶ所で圧入固定及び当接されているので、装着時のガタがなく、且つ、同軸心となるため、前記ピペットチップに試薬やさまざまな液体などを定量注入後、プレパラートや試験管などの容器へ的確に、且つ、正確に分注可能とすることが出来る。この時、前記テーパ内径部２ｃと前記ノズル外径部４ｂに隙間が形成されるが、前記エアタイト部（密閉）２ｇが円周上（リング状）に,且つ、帯状に設けられているので、液漏れなどは生じない。
本実施例では、ピペットチップ１はフィルター３を有しているが、注入時に薬液や異物が万が一、ピペット内へ進入するのを抑えるためで使用上、特に必要としない場合は外しても良い。
また、ノズル部装着時のガタ防止のために設けた円周リブ２ｈの断面形状は半円形状だが、三角形状、台形状、楕円形状でも良く、また全周ではなく、円周上に２点、４点、８点など多点に配置しても良く、上記、それぞれ組み合わせても良い。 Next, the usage method will be described. The nozzle portion 4a of the pipette 4 advances from the rear to the front and starts to be attached to the pipette tip 1. At this time, the taper angle α of the taper inner diameter portion 2e of the pipette tip body 2 is slightly larger than the taper angle β of the taper outer diameter portion 4b of the nozzle portion 4a, so that the nozzle portion 4a and the pipette Even if the axial center of the tip 1 is displaced, the corner 4c disposed in front of the tapered outer diameter portion 4b is guided to the tapered inner diameter portion 2e, and is inserted without being damaged along the tapers. The axes are aligned little by little. Further, when the nozzle 4a is advanced, the tapered inner diameter portion 2e and the front tapered outer diameter portion 4d of the tapered outer diameter portion 4b are press-fitted and fixed, and the tapered inner diameter portion 2e and the front tapered outer diameter portion 4d are shafts. An air tight portion (sealed) 2g is provided on the circumference (ring shape) with respect to the center and in a band shape. That is, the taper inner diameter portion 2e becomes an air tight portion (sealed) 2g. At the time of use, air flows in and out of the air tight part (sealed) 2 g and does not hinder the function (quantitative injection, extraction) of the pipette 4. At the same time, the circumferential rib 2h disposed behind the tapered inner diameter portion 2e and the rear tapered outer diameter portion 4e of the tapered outer diameter portion 4b are circumferentially (ring-shaped) with respect to the axis, and The contact portion 2i is fitted in a band shape with light press-fitting.
As a result, the pipette tip 1 and the nozzle portion 4a are press-fitted, fixed and brought into contact with each other at two locations, so that there is no backlash at the time of mounting and a coaxial center. Can be dispensed accurately and accurately into containers such as preparations and test tubes. At this time, a gap is formed between the tapered inner diameter portion 2c and the nozzle outer diameter portion 4b, but the air tight portion (sealed) 2g is provided on the circumference (ring shape) and in a band shape, Liquid leakage does not occur.
In this embodiment, the pipette tip 1 has the filter 3, but it may be removed if it is not particularly necessary for use in order to prevent a chemical solution or a foreign substance from entering the pipette at the time of injection.
The cross-sectional shape of the circumferential rib 2h provided to prevent looseness when the nozzle is mounted is semicircular, but it may be triangular, trapezoidal, or elliptical, and two points on the circumference, not the entire circumference. It may be arranged at multiple points such as 4 points, 8 points, etc., and may be combined as described above.

１ ピペットチップ
２ ピペットチップ本体
２ａ 後方開口部
２ｂ 段部
２ｃ 内径部
２ｄ たてリブ
２ｅ テーパ内径部
２ｆ 円周溝
２ｇ エアタイト部（密閉）
２ｈ 円周リブ
２ｉ 当接部
３ フィルター
３ａ 端面
３ｂ 外径部
４ ピペット
４ａ ノズル部
４ｂ テーパ外径部
４ｃ 角部
４ｄ 前方テーパ外径部
４ｅ 後方テーパ外径部
Ｔ リブ巾
Ｕ 肉厚
α テーパ角度
β テーパ角度 DESCRIPTION OF SYMBOLS 1 Pipette tip 2 Pipette tip main body 2a Back opening part 2b Step part 2c Inner diameter part 2d Vertical rib 2e Taper inner diameter part 2f Circumferential groove 2g Air tight part (sealed)
2h Circumferential rib 2i Contact part 3 Filter 3a End surface 3b Outer diameter part 4 Pipette 4a Nozzle part 4b Taper outer diameter part 4c Corner part 4d Front taper outer diameter part 4e Rear taper outer diameter part T Rib width U Thickness α Taper angle β taper angle

Claims (1)

ピペットチップとそのピペットチップに装着されるノズルとの連結構造であって、そのノズルのノズル部の外径のテーパ角度より、前記ピペットチップの後方内径部のテーパ角度を大きく形成すると共に、前記ノズル部の先端側に円周上のエアタイト部を設け、且つ、前記ピペットチップの後方の内径部に円周リブを設けたピペットチップとそのピペットチップに装着されるノズルとの連結構造。 The pipette tip is connected to a nozzle attached to the pipette tip, and the taper angle of the rear inner diameter portion of the pipette tip is larger than the taper angle of the outer diameter of the nozzle portion of the nozzle, and the nozzle The pipette tip which provided the air tight part on the circumference in the tip side of the part, and provided the circumference rib in the inner diameter part of the back of the pipette tip, and the connection structure of the nozzle with which the pipette tip is attached.
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JP2018130658A (en) * 2017-02-14 2018-08-23 住友ベークライト株式会社 pipette
CN112399888B (en) * 2018-05-11 2022-06-07 拜欧迪克斯公司 Pipette tip
USD983399S1 (en) 2018-05-11 2023-04-11 Biotix, Inc. Pipette tip
USD983400S1 (en) 2018-05-11 2023-04-11 Biotix, Inc. Pipette tip
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JP7189968B2 (en) 2018-05-31 2022-12-14 エッペンドルフ アーゲー pipette tip
JP2021531156A (en) * 2018-05-31 2021-11-18 エッペンドルフ アーゲー Pipette tip
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JP2020131092A (en) * 2019-02-15 2020-08-31 住友ベークライト株式会社 Pipette filter

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