JP2017219526A - Syringe type sensor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a sensor capable of simply and rapidly measuring a concentration of the component by simply collecting a solution to be inspected when required.SOLUTION: A syringe sensor optically measures concentration of the component of a solution to be inspected, and includes: an external cylinder having an inlet of the solution to be inspected at the top end and opened at the other end; and a piston partially reciprocable from an opening side into the external cylinder and fluid-tightly inserted. The top end part of a liquid-contacting side of the piston is attached with a fluorescent probe including a fluorescent material for emitting fluorescent light corresponding to the concentration of the component of the solution to be inspected by irradiated exciting light so as to come into contact each other. In the piston, an irradiation end part of the exciting light to the fluorescent probe and a light reception end part of the fluorescent light from the fluorescent probe are arranged on the opposite side of the liquid-contacting side of the fluorescent probe. The irradiation end part and the light reception end part are connected to light emitting and receiving/measurement means of the external side of the piston through inside of the piston.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a sensor that optically measures the concentration of a component of a test solution, for example, oxygen concentration, carbon dioxide concentration, hydrogen ion concentration (pH), and the like, and particularly relates to a syringe type sensor that can be easily measured.

  Concentrations (characteristics) of components of test liquids such as reaction solutions and culture solutions, such as oxygen (dissolved oxygen) concentration, carbon dioxide (dissolved carbon dioxide) Concentration, hydrogen ion concentration, etc. are measured and appropriate management of environmental conditions such as reaction and culture is performed. One of these measuring means uses a fluorescent probe containing a fluorescent substance (luminescent substance). There are sensors that measure optically.

As such an optical measurement sensor, for example, a flow cell type sensor equipped with a fluorescent probe that measures characteristics such as oxygen concentration while extracting a test solution from a culture tank with a pump is known.
In addition, the present applicant, together with other patent applicants, a fluorescent substance that emits fluorescence corresponding to the concentration of the component on the culture solution side wall of the sensor connector that can be fitted to the port provided on the side surface of the culture tank. A sensor has been proposed in which a fluorescent probe is fixed, and excitation light is emitted and fluorescence is received through an optical fiber whose tip is in contact with the opposite wall of the culture solution (see Patent Document 1). .

  JP 2014-135940 A

However, in the case of the above-described flow cell type sensor, when measuring characteristics such as oxygen concentration, there is a certain distance between the culture tank and the sensor, and thus a certain predetermined amount of test liquid is required. .
Moreover, in the case of the sensor comprised by the sensor connector of an above-mentioned culture tank, the port for exclusive use is required on the side surface of a culture tank.
Furthermore, there is a demand to easily collect such test liquids when necessary and to measure the concentration of the components easily and quickly to optimize the environmental conditions such as reaction and culture. .

  The present invention has been made in order to solve the above-mentioned problems and to meet demands. The purpose of the present invention is to simply collect a test solution when necessary and to determine the concentration of the component. An object of the present invention is to provide a sensor that can be measured easily and quickly with a small amount of test liquid.

  As a result of intensive studies to achieve the above-mentioned object, the present inventors usually measured the concentration of the components of the test liquid, usually on the test liquid collection port or top plate provided on the side of the culture tank or the like. A sample solution is sucked and collected with an outer cylinder of a syringe (syringe) and a piston through a short hose etc. that is thinner than a septum for collecting the sample solution provided, and at the tip of the piston on the liquid contact side A fluorescent probe containing a fluorescent substance (luminescent substance) that emits fluorescence corresponding to the concentration of the component of the test solution is irradiated with the irradiated excitation light so as to be in contact with the fluorescent probe, and the excitation light to the fluorescent probe is attached. The concentration of the component is measured at the same time that the irradiation and the light reception of the fluorescence from the fluorescent probe are performed by the light emitting / receiving / measuring device outside the piston through the light emitting end and the light receiving end disposed in the piston. To reduce the concentration of the component Optically simple, yet quickly obtain a new knowledge such that may measure, the present invention has been completed based on these findings.

That is, the present invention includes the following inventions.
(1) A syringe-type sensor for optically measuring the concentration of a component of a test liquid, the outer cylinder having a suction port for the test liquid at the tip and having the other end opened, and opening in the outer cylinder A piston part of which is reciprocally movable from the side and inserted in a liquid-tight manner. The tip of the piston on the liquid contact side is adjusted to the concentration of the component of the test liquid by irradiated excitation light. A fluorescent probe containing a fluorescent substance that emits corresponding fluorescence is mounted so that the fluorescent substance comes into contact with the fluorescent substance, and an irradiation end of the excitation light to the fluorescent probe and the fluorescent probe are installed in the piston. The fluorescent light receiving end from the fluorescent probe is disposed opposite to the liquid contact side of the fluorescent probe, and the irradiation end and the light receiving end pass through the piston and receive and emit light outside the piston. The sensor connected to a measuring device.

(2) The irradiation end and the light receiving end are tip portions of an optical transmission medium penetrating the piston in the axial direction, and the other end of the optical transmission medium is connected to the light emitting / receiving / measurement device via an optical fiber. The sensor according to (1), which is optically connected.

(3) The sensor according to (2), wherein the optical transmission medium is light transmissive glass or plastic, or an optical fiber.

(4) The irradiation end portion is a light emitting element, and the light receiving end portion is a light receiving element, and a lead wire included in each element is electrically connected to the light receiving and emitting / measuring device. ) Sensor.

(5) The sensor according to (4), wherein the light emitting element is a light emitting diode, and the light receiving element is a photodiode.

(6) Any one of (1) to (5), wherein the other end of the piston is provided with a connector mounting portion, and the irradiation end portion and the light receiving end portion are connected to the light emitting / receiving / measurement device by the connector. Sensor.

(7) The sensor according to any one of (1) to (6), wherein the fluorescent probe is for measuring an oxygen concentration, a carbon dioxide gas concentration, or a hydrogen ion concentration.

  According to the present invention, the sample liquid is aspirated and collected with the outer cylinder of the syringe and the piston through a hose that is thinner and shorter than the port provided on the side wall of the culture tank or the like or the septum provided on the top plate, etc. A fluorescent probe containing the above-described fluorescent substance (luminescent substance) is attached to the tip of the liquid contact side of the piston so as to be in contact with the liquid, and the fluorescent probe is irradiated with excitation light and the fluorescence from the fluorescent probe. Is received by a light emitting / receiving / measuring device outside the piston via a light emitting end and a light receiving end arranged in the piston, and the concentration of the component is measured at the same time. It is possible to provide a syringe type sensor that can be easily collected and the concentration of the component can be measured optically simply and quickly with a small amount of test liquid.

These are the longitudinal cross-sectional views which show 1st Embodiment of the syringe type sensor of this invention. These are the longitudinal cross-sectional views which show one Embodiment of the fluorescent probe of the syringe type sensor of this invention. These are the longitudinal cross-sectional views which show 2nd Embodiment of the syringe type sensor of this invention.

  As described above, the syringe-type sensor of the present invention (hereinafter sometimes simply referred to as a sensor) measures the concentration of a component of a test solution such as a collected chemical reaction solution or a culture solution of microorganisms or animal and plant cells. Used for.

First, the sensor of the present invention employs, in particular, a syringe (injection cylinder) system for collecting a test liquid, and a liquid contact with a piston (inner cylinder) inserted in the outer cylinder of the syringe accordingly. A fluorescent probe is attached to the tip of the side, and the irradiation end and the light receiving end are arranged in the piston for irradiating the fluorescent probe with the excitation light and receiving the fluorescent light from the fluorescent probe from the opposite side of the liquid contact side. It is a structural feature that is performed through the section.
The irradiation end portion and the light receiving end portion are a front end portion of an optical transmission medium such as an optical fiber, or a light emitting element and a light receiving element, respectively, and pass through (via) the piston. It is optically connected to the outer light emitting / receiving / measuring device, and in the latter case, it is electrically connected to the light emitting / receiving / measuring device.

As a general sensor for optically measuring the concentration of a component of a test liquid, for example, oxygen (dissolved oxygen) concentration, carbon dioxide (dissolved carbon dioxide) concentration, hydrogen ion concentration (hereinafter referred to as “pH”), etc. For example, a patch-type (chip, spot) fluorescent probe (a fluorescent substance (luminescent substance) having a property that the fluorescent characteristics change depending on the oxygen concentration, carbon dioxide concentration, and pH value) commercially available from Presense, Germany The applied patch shape (patch type) (for example, a thin thin piece having an appropriate shape such as a circular shape) and the fluorescent probe are irradiated with excitation light via an optical fiber, and the fluorescence from the fluorescent probe is received. There is known a system (sensor) in which a measuring instrument (light emitting / receiving / measuring device) provided with a light emitting / receiving unit is a separate component.
Further, a fluorescent dissolved oxygen sensor unit commercially available from Able Japan is known.

  The patch-type fluorescent probe described above is constituted by a technique such as applying or dispersing a fluorescent substance on the surface or inside of a light-transmitting transparent glass plate or resin plate, or a transparent resin film. The plate surface and film (sheet) surface of the fluorescent probe are attached to, for example, the inner wall of the culture tank in contact with the liquid, and the fluorescent material is disposed so as to come into contact with the liquid. In this case, when the excitation light is emitted from the light receiving / emitting / measuring device through the transparent culture vessel wall and the optical fiber from the outside of the pasting surface, the fluorescent substance is excited to emit fluorescence, and this fluorescence is transmitted through the optical fiber. It is possible to measure the concentration (characteristics) of components such as dissolved oxygen concentration, dissolved carbon dioxide concentration, pH, etc. by measuring the intensity (phase angle) of fluorescence while receiving light with the light emitting and receiving / measuring device. It is.

As the fluorescent probe and the light emitting / receiving / measuring device used in the sensor of the present invention, when the irradiation end portion and the light receiving end portion are the front end portion of an optical transmission medium such as an optical fiber, the above-mentioned commercially available products may be mentioned. .
For the light emitting / receiving / measuring device when the irradiation end and the light receiving end are a light emitting element and a light receiving element, respectively, the light emitting element and the light receiving element of a commercially available light emitting / receiving device as described above are arranged in the piston. By doing so, it can be configured without an optical fiber.

Hereinafter, the present invention will be described in more detail with reference to the drawings.
(First embodiment)
FIG. 1 is a longitudinal sectional view showing a first embodiment of the syringe type sensor of the present invention.
In the figure, a syringe-type sensor 1 has a cylindrical outer cylinder 2 having a test liquid suction port 2a at the tip and an opening at the other end, and a part of the outer cylinder 2 can reciprocate from the opening side. And a rod-like piston (inner cylinder) 3 inserted in a liquid-tight manner through a packing 3a such as an O-ring.

The suction port 2a of the outer cylinder 2 is connected to an elastic tube 2b made of, for example, silicon rubber for collecting the test liquid, but may be connected in advance.
The elastic tube 2b is preferably thin and short so as to minimize the amount of sample liquid to be collected.
In the sensor 1 of the present invention, the amount of the sample liquid to be collected should be 0.3 ml or more, and the concentration of the above components can be measured with a small amount of the sample liquid.
Further, when the sample liquid is collected, the measurement may be performed immediately after the suction port 2a side of the sensor 1 is directed upward and the piston 3 is slightly pushed upward to evacuate the air.

The material of the outer cylinder 2 and the piston 3 is not particularly limited, and examples thereof include synthetic resin, glass, stainless steel, and the like. For the piston, for example, stainless steel is preferable.
In addition, about the outer cylinder 2, the outer cylinder of a commercially available syringe is used effectively as it is.

For example, a circular patch type fluorescent probe 4 is attached to the tip 3b of the piston (material example: stainless steel) 3. The fluorescent probe 4 contains a fluorescent substance that emits fluorescence corresponding to the concentration of the component of the test liquid by the irradiated excitation light.
In this case, the fluorescent probe 4 is mounted so that the fluorescent substance is in contact with the test solution.
As described above, the fluorescent probe 4 may be used for measuring oxygen concentration, carbon dioxide concentration, pH, and the like.

In order to attach the fluorescent probe 4 to the tip 3b on the liquid contact side of the piston 3, an appropriate method is employed. For example, the peripheral part of the surface opposite to the liquid contact side of the patch-type fluorescent probe 4 is positioned at the peripheral part of the piston 3, and a gasket 10 a such as an O-ring is interposed between the peripheral parts of the cap nut 10 and the piston 3. By screwing with the outer periphery in the vicinity of the tip 3b, it can be fixed and mounted in a liquid-tight manner.
The size of the patch-type fluorescent probe may be approximately the same as the outer diameter of the piston tip 3b.

In the piston 3, a tip portion of a light transmission medium 5, which will be described later, is formed in a round bar shape penetrating the inside of the piston 3 in the axial direction, and serves as an irradiation end portion 6 of excitation light to the fluorescent probe 4. It is arranged to face the opposite side, and this tip part also functions as a light receiving end part 7 for fluorescence from the fluorescent probe 4.
The facing distance between the fluorescent probe and the irradiation end and the light receiving end is not particularly limited, but is preferably 0 mm (contact) to 2 mm.
In this aspect, since the excitation light irradiation and the fluorescence light reception are alternately performed at regular intervals as described later, the front end portion of the optical transmission medium 5 is connected to the irradiation end portion 6 and the light receiving end portion 7. I will also serve.

The irradiation end 6 and the light receiving end 7 (the tip of the optical transmission medium 5) are connected to a light emitting / receiving / measuring device (not shown) outside the piston 3 via the optical fiber 8 at the other end of the optical transmission medium 5. Optically connected. In this case, the mounting portion 9a of the optical connector 8a is provided at the end of the cylindrical mounting member (cap) 9 fixed to the other end of the piston 3 by screwing or the like, whereby the optical transmission medium is transmitted via the optical fiber 8. The other end of 5 and the light emitting / receiving / measuring device can also be connected.
In addition, 9b is a hollow part into which the optical fiber 8 to be connected is inserted.
The optical transmission medium 5 is preferably fixed in the piston 3 with an adhesive or the like.

The sensor 1 of the present invention can also be configured such that the optical fiber 8 connected to the light emitting / receiving / measuring device can be disconnected by the mounting portion 9a of the optical connector 8a. 1 is operated alone, and then the concentration of the component can be measured by attaching the optical connector 8a of the optical fiber 8 to the attachment portion 9a of the optical connector 8a, which is convenient in handling.
Of course, when the following coated optical fiber is used as the optical transmission medium 5, the optical transmission medium may be directly connected to the light emitting / receiving / measuring device without using the optical connector 8a.
Further, as will be described later, when the sensor 1 is subjected to steam sterilization treatment, a lid cap (not shown) can be screwed onto the mounting portion 9a in order to prevent steam from entering the piston 3.

FIG. 2 is a longitudinal sectional view showing another embodiment in which the fluorescent probe 14 is mounted so that the fluorescent substance comes into contact with the tip 3 b on the liquid contact side of the piston 3.
In this figure, at least the upper part of the fluorescent probe cap 20 having a screw on the inner periphery is made of a light-transmitting transparent material, such as polycarbonate, and the fluorescent probe 14 provided on the upper surface of the cap has a light transmission property. It is fixed with an adhesive such as a silicon adhesive.
Then, the fluorescent probe cap 20 and the outer periphery in the vicinity of the tip 3b of the piston 3 are screwed together via a gasket such as an O-ring so that the fluorescent probe 4 is in contact with the tip 3b on the liquid contact side of the piston 3. It is fixed in a liquid-tight manner.
In this case, the thickness of the upper portion of the fluorescent probe cap 20 to which the fluorescent probe 14 is fixed affects the measured fluorescence intensity, and is preferably less than about 3 mm.

Furthermore, as another method of providing the fluorescent probe 4 at the tip 3b of the piston 3, a method of directly applying a fluorescent material to the tip of the light transmission member 5 located at the tip 3b of the piston 3 can be adopted.
An example of producing a fluorescent probe will be described later.

Examples of the optical transmission medium 5 in which the tip of the fluorescent probe 4 is disposed facing the fluorescent probe 4 in the piston 3 include transparent glass (example: quartz glass) and transparent plastic (example: fully fluorinated polymer, polycarbonate). Etc.), a double-structured optical fiber (glass or plastic optical fiber) composed of a core and a clad, an optical fiber coated with this dual structure, and the like are preferred, and these optical fibers are particularly preferred.
In addition, since the optical transmission medium 5 penetrates through the piston 3, the material of the piston 3 is preferably a light-shielding material such as stainless steel. However, in the case of a light-transmitting material, the above-described material is used. Apart from the coated optical fiber, the light transmission medium 5 may be coated or coated with a light shielding material, for example.

The thickness of the optical transmission medium 5 is not particularly limited, but the diameter (in the case of an optical fiber, the diameter of the double structure of the core and the clad) is preferably, for example, 1 to 3 mm.
Further, the diameter of the optical fiber 8 connected to the optical transmission medium 5 is not particularly limited, but the diameter of the double structure of the core and the clad excluding the coating is preferably 1 to 3 mm, for example. .

When the sensor 1 of the present invention is used to collect, for example, a culture solution cultured while preventing contamination by various bacteria or a culture solution in the middle of culture as a test solution, steam such as 30 minutes at 121 ° C. Those that allow sterilization are preferred.
At this time, a heat-resistant member may be employed as a member constituting the sensor 1.
Note that the fluorescent probe of the following production example can be subjected to steam sterilization, and can be steam sterilized by using glass or a double-structured optical fiber made of glass as the optical transmission member.

  Thus, the syringe type sensor 1 of the present invention is an effective sensor that can easily and quickly measure the concentration of the component with a small amount of the test liquid by simply collecting the test liquid when necessary.

(Second Embodiment)
FIG. 3 is a longitudinal sectional view showing a second embodiment of the syringe type sensor of the present invention.
In this embodiment, substantially the same members / locations as those in the first embodiment are denoted by the same reference numerals, and description thereof is omitted.
In the figure, a syringe type sensor 21 is different from the sensor 1 of the first embodiment in that an irradiation end portion 26 and a light receiving end portion 27 in the piston 3 are a light emitting element and a light receiving element, respectively.

  In the sensor 21, the fluorescent probe 4 is mounted in a liquid-tight manner on the tip 3 b of the piston 3 inserted into the outer cylinder 2 in the same manner as described in the first embodiment.

In the piston 3, an irradiation end portion 26 of excitation light to the fluorescent probe 4 and a light receiving end portion 27 of fluorescence from the fluorescent probe 4 are arranged opposite to the opposite side of the fluorescent probe 4 on the liquid contact side.
The irradiation end portion 26 and the light receiving end portion 27 are fixed to the irradiation end portion 26 and the light receiving end portion 27 by an appropriate support member 31 in a hollow portion 30 penetrating the piston 3 in the axial direction. The lead wires 26a, 27a (two each) provided are terminals 32 (terminal connector terminals) provided at the end of a cylindrical mounting portion (cap) 9 fixed to the other end of the piston 3 by screwing or the like. It is electrically connected to the light emitting / receiving / measuring device (not shown) via the mounting portion 9a).
As the irradiation end portion 26, for example, a light emitting element such as a light emitting diode (LED) is used. As the light receiving end portion 27, for example, a light receiving element such as a photodiode (PD) is used.

  As described in the first embodiment, the sensor 21 of the present invention is effective in that a test solution can be easily collected when necessary and the concentration of the component can be easily and quickly measured with a small amount of the test solution. Sensor.

  Here, the culture solution (test solution) collected in the outer cylinder 2 from the port of the culture tank or the like via the elastic tube 2b of the sensor 1 of the present invention in the first embodiment is as follows. A method for measuring oxygen concentration using a fluorescent probe for measuring oxygen concentration will be described as an example, but this measurement method itself is known.

  Using a known light emitting / receiving device (for example, Fibox 3 (trade name made by Germany Presense), DBF-1131 (trade name made by Able, Japan), etc.), the tip 3b of the piston 3 is covered. From the irradiation end 6 of the tip of the optical transmission medium 5 to the fluorescent probe 4 in contact with the test solution via the optical fiber 8 and the optical transmission medium 5 from the light emitting / receiving / measuring device, for example, wavelengths 400 to The excitation light of 600 nm or the like has an appropriate irradiation time, for example, 500 milliseconds (msec.) To 1 second (sec.), And an appropriate irradiation interval, for example, 500 milliseconds (msec.) To 60 seconds (sec.). ) Etc. and intermittently irradiated.

The fluorescence emitted from the fluorescent material of the fluorescent probe 4 excited by the irradiation of the excitation light is received at the tip of the optical transmission medium 5 during the time period (between irradiation and the next irradiation) when the excitation light is not irradiated. The light is received at the end 7 and transmitted to the light emitting / receiving / measuring device, and the light intensity (phase angle) is measured intermittently (in the light emitting / receiving / measuring device, irradiation with excitation light and light reception are repeated alternately).
At this time, since the emission intensity is reduced due to the presence of oxygen, the oxygen concentration is calculated and measured from the measured value of the received light intensity and the calibration curve of the known oxygen concentration separately prepared in advance. Become.

Next, an example of manufacturing a fluorescent probe will be described.
As the fluorescent probe used in the present invention, as described above, a known probe coated with a fluorescent substance that emits fluorescence corresponding to the concentration of the component of the test solution is effectively used. Will describe a fluorescent probe for measuring oxygen concentration, which is an example of the previously proposed fluorescent probe (see Japanese Patent Application Laid-Open No. 2014-153348).

Example of Preparation of Fluorescent Probe for Oxygen Concentration Measurement (1) Fluorescent substance (luminescent substance) PtT975 (trade name, platinum (II) -tetra (pentafluorophenyl) porphyrin (Pt (US Frontier Scientific)) II) 5 ml of organic solvent acetone was added to 5.6 mg of meso-Tetra (pentafluorophenyl) Porphine) (metal porphyrin complex) and stirred to prepare a suspension.

(2) Put 260 mg of polycarbonate (PC) granules obtained by the following method in a dedicated test tube of a rotary evaporator, preheat the PC to 60 ° C with hot air from a blower while rotating, and then perform the test The suspension prepared in (1) is quickly put into a tube while rotating, and immediately heated from outside the test tube with hot air from a blower so that the temperature in the test tube becomes about 70 ° C., and acetone is added for 10 seconds in contact time. A granular preparation was prepared, which was rapidly vaporized and the fluorescent material adhered and fixed to the surface of the PC granules in an appropriate dispersion state.

Transparent polycarbonate (PC) powdered:
8 g of PC chips were taken in an automatic mortar and pulverized at 300 rpm for 5.5 hours, and then the granules adhered to the inside of the mortar and the like were collected to obtain PC granules.
Subsequently, for this PC granule, a stainless steel 200-mesh (aperture 75 μm) sieve granule was collected, and the non-passed granule was returned to the mortar and further pulverized at 300 rpm for 1 hour. In the same manner, granules passing through a 200 mesh sieve were collected.
These were combined into a PC granule.

(3) A light-transmitting and oxygen-permeable synthetic adhesive (example: silicon RTV rubber (Momentive Performance Performance) is added to the granular preparation of the fluorescent substance prepared in (2) and PC granules. Materials Japan GK TSE389W)) was added and mixed quickly in about 1 minute so as to be uniform to prepare a mixture.

(4) FEP film (tetrafluoroethylene / hexafluoropropylene copolymer) having a thickness of 2 mil (0.05 mm) as a light-transmitting film (example: Neofron FEP film (single-side hydrophilization treatment) manufactured by Daikin Industries, Ltd.) ) Using NF-0050B-1), thinly apply the mixture prepared in (3) to the treated surface, and then avoid solid dusting and leave it at room temperature for 24 hours or more to completely solidify A solidified product was obtained.

(5) The solidified product obtained in (4) was molded into a circular-like patch type (example: circular patch having a diameter of 4 mm) by an appropriate method using a punch or the like to produce a fluorescent probing with a thin film thickness.
The response speed of the thus prepared fluorescent probe was 95%, which was good at 60 seconds.
The response speed indicating the performance of the fluorescent probe is measured at two points: an oxygen concentration in the measurement cell (container) of 0% (air is completely replaced with nitrogen gas) and an oxygen concentration of 21% (air 100%). .

  Moreover, about the measuring method of the oxygen concentration using the sensor 21 of the present invention in the second embodiment, as described above, the sensor 21 is configured so that the light emitting element and the light receiving element of the light emitting / receiving / measuring device are respectively irradiated end portions in the piston. The configuration is substantially the same as that of the sensor 1 except that the light receiving element and the light receiving element are repositioned, and the light emitting element and the lead wire of the light receiving element are electrically connected to the light receiving / emitting / measuring device. Therefore, the oxygen concentration can be measured in the same manner as in the method of measuring the oxygen concentration using the sensor 1.

DESCRIPTION OF SYMBOLS 1, 21 Syringe type sensor 2 Outer cylinder 2a Suction port 2b Elastic pipe 3 Piston 3a Packing 3b Tip part 4, 14 Fluorescent probe 5 Optical transmission medium 6, 26 Irradiation end part 7, 27 Light reception end part 8 Optical fiber 8a Optical connector 9 Attachment Member (cap)
9a Mounting portion 9b Hollow portion 10 Cap nut 20 Cap for fluorescent probe 26a Lead wire 27a Lead wire 30 Hollow portion 31 Support member 32 Terminal

Claims (7)

A syringe type sensor that optically measures the concentration of a component of a test liquid,
An outer cylinder having a suction port for the test liquid at the tip and having the other end opened;
A piston part of which is reciprocally movable in the outer cylinder from the opening side and is fluid-tightly inserted;
With
A fluorescent probe containing a fluorescent substance that emits fluorescence corresponding to the concentration of the component of the test liquid by irradiated excitation light is in contact with the front end of the piston on the liquid contact side. Is installed,
In the piston, an irradiation end portion of the excitation light to the fluorescent probe and a light receiving end portion of the fluorescence from the fluorescent probe are arranged opposite to the liquid contact side of the fluorescent probe,
The sensor, wherein the irradiation end and the light receiving end are connected to a light emitting / receiving / measuring device outside the piston through the piston.   The irradiation end portion and the light receiving end portion are tip portions of an optical transmission medium that penetrates the piston in the axial direction, and the other end of the optical transmission medium is optically connected to the light emitting / receiving / measurement device via an optical fiber. The sensor of claim 1 connected.   The sensor according to claim 2, wherein the optical transmission medium is light transmissive glass or plastic, or an optical fiber.   The said irradiation end part is a light emitting element, Moreover, the said light receiving end part is a light receiving element, The lead wire with which each element is equipped is electrically connected to the said light emitting / receiving and measuring apparatus. Sensor.   The sensor according to claim 4, wherein the light emitting element is a light emitting diode, and the light receiving element is a photodiode.   The other end of the piston is provided with a connector mounting portion, and the irradiation end portion and the light receiving end portion are connected to the light emitting / receiving / measurement device by the connector. Sensor.   The sensor according to claim 1, wherein the fluorescent probe is used for measuring an oxygen concentration, a carbon dioxide concentration, or a hydrogen ion concentration.

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