JP2017219337A - Method for measuring ionization degree of carboxyl group of hair protein - Google Patents
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 49
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 36
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 title claims abstract description 27
- XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims abstract description 26
- 239000000126 substance Substances 0.000 claims abstract description 15
- 238000004566 IR spectroscopy Methods 0.000 claims abstract description 10
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 8
- 239000001257 hydrogen Substances 0.000 claims abstract description 8
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 claims abstract description 7
- 229910052805 deuterium Inorganic materials 0.000 claims abstract description 7
- 238000000862 absorption spectrum Methods 0.000 claims abstract description 4
- 238000011282 treatment Methods 0.000 claims description 26
- 238000005259 measurement Methods 0.000 claims description 6
- 238000000691 measurement method Methods 0.000 claims description 3
- 101710196022 Cuticle protein Proteins 0.000 claims description 2
- 150000001408 amides Chemical class 0.000 abstract description 9
- 125000000524 functional group Chemical group 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000004061 bleaching Methods 0.000 description 8
- 239000007844 bleaching agent Substances 0.000 description 5
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 230000037308 hair color Effects 0.000 description 3
- 230000003699 hair surface Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- SFNALCNOMXIBKG-UHFFFAOYSA-N ethylene glycol monododecyl ether Chemical compound CCCCCCCCCCCCOCCO SFNALCNOMXIBKG-UHFFFAOYSA-N 0.000 description 2
- 238000001028 reflection method Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 239000000118 hair dye Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229940057950 sodium laureth sulfate Drugs 0.000 description 1
- SXHLENDCVBIJFO-UHFFFAOYSA-M sodium;2-[2-(2-dodecoxyethoxy)ethoxy]ethyl sulfate Chemical compound [Na+].CCCCCCCCCCCCOCCOCCOCCOS([O-])(=O)=O SXHLENDCVBIJFO-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
本発明は毛髪タンパク質のカルボキシ基のイオン化度測定方法に関し、詳しくは、赤外吸収分光法を用いた毛髪タンパク質のカルボキシ基のイオン化度測定方法に関する。 The present invention relates to a method for measuring the degree of ionization of a carboxy group of a hair protein, and more particularly to a method for measuring the degree of ionization of a carboxy group of a hair protein using infrared absorption spectroscopy.
メラニン色素を分解し毛髪の色を明るくするブリーチ処理や毛髪の色を変える染毛処理等の毛髪に対する化学処理が古くから行われている。しかしながら、ブリーチ処理等の化学処理はメラニン色素だけではなく、毛髪の他の組織にも化学的な変化を及ぼす。特に毛髪組織を形成する主要成分である毛髪タンパク質に与える影響は重要である。また、毛髪組織は性質の異なるキューティクル、コルテックス、メデュラよりなる階層構造を取っており、ブリーチ処理等の化学処理の影響を捉えるためには層構造ごとに検討を行う必要がある。 Chemical treatments for hair have been performed for a long time, such as bleaching treatment for decomposing melanin and lightening hair color, and hair coloring treatment for changing hair color. However, chemical treatments such as bleaching treatment not only affect melanin pigment but also other hair tissues. In particular, the effect on hair proteins, which are the main components that form hair tissue, is important. Further, the hair tissue has a hierarchical structure composed of cuticles, cortex, and medura having different properties, and in order to capture the influence of chemical treatment such as bleaching treatment, it is necessary to examine each layer structure.
ブリーチ処理を行った毛髪ではキューティクルの浮きや剥がれが観察され、毛髪の滑り性が低下、すなわち摩擦抵抗が増加することも知られている（非特許文献１）。しかし、毛髪タンパク質の化学的変化についての知見は乏しい。 It is also known that hair that has undergone bleaching treatment is observed to have a cuticle that floats or peels off, and that the slipperiness of the hair decreases, that is, the frictional resistance increases (Non-Patent Document 1). However, little is known about the chemical changes in hair proteins.
毛髪タンパク質のような繊維状のタンパク質の性質を調べる方法としては赤外吸収分光法やラマン散乱分光法等が簡便な方法として良く用いられている。例えば、タンパク質の主要構造であるアミド結合について、アミドI、アミドII、アミドIII等の特徴的な信号が測定され、アミド結合の性質が検討される。 Infrared absorption spectroscopy, Raman scattering spectroscopy, and the like are often used as simple methods for investigating the properties of fibrous proteins such as hair proteins. For example, characteristic signals of amide I, amide II, amide III, etc. are measured for the amide bond which is the main structure of the protein, and the nature of the amide bond is examined.
しかしその一方で、アミドI、アミドII、アミドIIIの特徴的な信号はその信号強度が非常に強い場合が多く、ほかの化学結合、官能基の情報を測定しようとする際の大きな妨害となることがしばしばである。毛髪の化学処理によって毛髪タンパク質中のカルボキシ基に与えている影響について検討を試みようとしても、上記のアミド結合由来の信号が非常に強く、その検討は困難な現状にある。 However, on the other hand, the characteristic signals of amide I, amide II, and amide III are often very strong in signal intensity, which is a major obstacle when trying to measure information on other chemical bonds and functional groups. Often it is. Even if an attempt is made to examine the influence of hair chemical treatment on the carboxy group in the hair protein, the signal derived from the amide bond is very strong, and the examination is difficult.
毛髪タンパク質の化学的性質を評価する上で重要な毛髪タンパク質のカルボキシ基の状態を測定する方法を見出そうとするものである。 The present invention seeks to find a method for measuring the state of the carboxy group of a hair protein, which is important in evaluating the chemical properties of the hair protein.
本発明者は、これらの従来の問題点を解決するために鋭意検討した結果、毛髪に水素／重水素交換を行い、赤外吸収分光法によって測定されるＣＯＯ−逆対称伸縮振動の信号強度を用いることにより、これまでの問題点を解決できることを見出し、本発明を完成するに至った。 As a result of intensive studies to solve these conventional problems, the present inventor conducted hydrogen / deuterium exchange on the hair, and obtained the signal intensity of the COO - inverse symmetric stretching vibration measured by infrared absorption spectroscopy. By using it, it discovered that the conventional problem could be solved, and came to complete this invention.
すなわち、本発明は、毛髪に重水を接触させて水素／重水素交換を行う工程と、前記被測定物質を赤外吸収分光法によって測定する工程と、を含む測定方法を用いて毛髪タンパク質について測定した赤外吸収スペクトルのうち、１５８０ｃｍ−１付近のＣＯＯ−逆対称伸縮振動の信号強度を測定することを特徴とする毛髪タンパク質のカルボキシ基のイオン
化度測定方法である。
That is, the present invention measures hair protein using a measurement method comprising a step of bringing hydrogen into contact with heavy water to perform hydrogen / deuterium exchange, and a step of measuring the substance to be measured by infrared absorption spectroscopy. It is the ionization degree measuring method of the carboxy group of the hair protein characterized by measuring the signal strength of COO - inverse symmetric stretching vibration in the vicinity of 1580 cm −1 in the infrared absorption spectrum obtained.
本発明によれば、毛髪タンパク質のカルボキシ基のイオン化度を簡便に測定することができる。毛髪を非破壊的に測定することも可能になる。 According to the present invention, the ionization degree of a carboxy group of a hair protein can be easily measured. It is also possible to measure the hair non-destructively.
以下、本発明を詳細に説明する。 Hereinafter, the present invention will be described in detail.
本発明の毛髪タンパク質のカルボキシ基のイオン化度測定方法は、毛髪に重水を接触させて水素／重水素交換を行う工程と、前記被測定物質を赤外吸収分光法によって測定する工程とを含む。ここで、毛髪と重水との接触は、重水を毛髪に滴下する方法、重水中に毛髪を浸漬させる方法、重水蒸気下に毛髪を放置する方法などがある。 The method for measuring the degree of ionization of a carboxy group of a hair protein of the present invention includes a step of bringing heavy water into contact with hair to perform hydrogen / deuterium exchange, and a step of measuring the substance to be measured by infrared absorption spectroscopy. Here, the contact between the hair and heavy water includes a method of dripping heavy water into the hair, a method of immersing the hair in heavy water, a method of leaving the hair under heavy water vapor, and the like.
上記工程を経て毛髪タンパク質について測定した赤外吸収スペクトルのうち、１５８０ｃｍ−１付近のＣＯＯ−逆対称伸縮振動の信号強度を測定する。赤外吸収分光法としては、ＡＴＲ法、透過法、反射吸収法、外部反射法、拡散反射法、錠剤法などがある。ＡＴＲ法は毛髪を非破壊的に測定する方法として好ましい。 Of the infrared absorption spectrum measured for the hair protein through the above steps, the signal intensity of the COO - inverse symmetric stretching vibration near 1580 cm −1 is measured. Examples of infrared absorption spectroscopy include ATR method, transmission method, reflection absorption method, external reflection method, diffuse reflection method, and tablet method. The ATR method is preferable as a method for nondestructively measuring hair.
ＡＴＲ法では、毛髪タンパク質の内でキューティクルタンパク質の測定に適している場合が多い。非破壊的に経時的な測定が可能であり、また、同一毛髪を繰り返し処理した後に測定することも可能である。 The ATR method is often suitable for measurement of cuticle protein among hair proteins. Non-destructive measurement over time is possible, and it is also possible to measure after repeatedly processing the same hair.
毛髪タンパク質が化学処理された毛髪タンパク質である場合、毛髪タンパク質のカルボキシ基に変化が起こる場合が多く、本発明の毛髪タンパク質のカルボキシ基のイオン化度測定方法により測定が可能である。 When the hair protein is a chemically treated hair protein, the carboxy group of the hair protein often changes, and can be measured by the method for measuring the degree of ionization of the carboxy group of the hair protein of the present invention.
化学処理としては、ブリーチ処理、染毛処理、パーマ処理等が挙げられる。また、酸またはアルカリによる処理等も含まれる。 Examples of the chemical treatment include bleach treatment, hair dye treatment, and permanent treatment. Moreover, the process by an acid or an alkali is included.
上記化学処理を行ったことがない毛髪を健康な毛髪とする。 Hair that has not been subjected to the chemical treatment is defined as healthy hair.
化学処理を行ったことがない健康な毛髪を用い上記方法にて得た信号強度をＩＨ、化学処理された毛髪を用い上記方法にて得た信号強度をＩＣとからイオン化ダメージ度（ＩＣ−ＩＨ）／ＩＨを算出して、毛髪タンパク質のカルボキシ基のイオン化ダメージ度を求める。 The signal intensity obtained by the above method using healthy hair that has not been chemically treated is I H , and the signal intensity obtained by the above method using chemically treated hair is obtained from I C based on the ionization damage degree (I C− I H ) / I H is calculated to determine the degree of ionization damage of the carboxy group of the hair protein.
また毛髪タンパク質が化学処理する前後でのそれぞれの信号強度をＩ０、Ｉｎとするときにこれらからイオン化ダメージ度（Ｉｎ−Ｉ０）／Ｉ０を算出して、毛髪タンパク質のカルボキシ基のイオン化ダメージ度を求める。 The respective signal intensities of before and after the hair protein is chemically treated by calculating them from the ionization damage value (I n -I 0) / I 0 when the I 0, I n, the carboxy group of the hair protein Determine the degree of ionization damage.
以下に、実施例により本発明をより詳細に説明するが、本発明はこれによってなんら限定されるものではない。 Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.
健康な人毛黒髪の毛束（２０ｃｍ、２ｇ）を８質量％ラウレス硫酸ナトリウム水溶液で洗浄後、市販のブリーチ剤で３回処理した後に自然乾燥させたブリーチ処理毛髪（ＨＢ）、健康な人毛黒髪の毛束（２０ｃｍ、２ｇ）を８質量％ラウレス硫酸ナトリウム水溶液で洗浄後、市販のパウダーブリーチ剤で１回処理した後に自然乾燥させたパウダーブリーチ処理毛髪（ＨＰ）と、健康な人毛黒髪の毛束（２０ｃｍ、２ｇ）を８質量％ラウレス硫酸ナ
トリウム水溶液で洗浄後に自然乾燥させた健康毛髪（ＨＨ）を調製した。
Bleached hair (HB), which is a natural human hair, washed with 8% by weight sodium laureth aqueous solution and then dried with a commercially available bleaching agent, and then naturally dried. Hair bleached hair (HP) that was washed with 8% by weight aqueous sodium laureth sulfate solution (20cm, 2g), then treated with a commercially available powder bleaching agent and then naturally dried, and healthy human hair Healthy hair (HH) was prepared by washing (20 cm, 2 g) with an 8% by mass sodium laureth aqueous solution and then naturally drying.
次に、上記各々の毛束を二分割し、一方を重水中に一昼夜浸漬した後に自然乾燥させ、各々の水素／重水素交換毛髪（ＤＢ、ＤＰ、ＤＨ）を調製した。 Next, each of the above-mentioned hair bundles was divided into two, and one was immersed in heavy water all day and night, and then naturally dried to prepare each hydrogen / deuterium exchange hair (DB, DP, DH).
赤外吸収分光測定はサーモサイエンティフィック社製のＮｉｃｏｌｅｔ ｉＺ１０を用い、ＡＴＲ法（ダイヤモンドプリズム、入射角４５°、１回反射型、バックグラウンドは空気）にて測定領域を８００〜４０００ｃｍ−１とし、積算回数３２回、分解能４ｃｍ−１にて行った。 The infrared absorption spectroscopic measurement uses Nicolet iZ10 made by Thermo Scientific, and the measurement area is set to 800 to 4000 cm −1 by the ATR method (diamond prism, incident angle 45 °, single reflection type, background is air). The number of times of integration was 32 and the resolution was 4 cm −1 .
本測定による赤外光の潜り込み深さは約１．５μｍである。同時に測定した各毛髪のキューティクル層の厚さ及び枚数には有意差はなく約３μｍ、６〜７枚であったことこら、上記方法により得られる信号は毛髪キューティクル層に由来するものである。 The penetration depth of infrared light by this measurement is about 1.5 μm. The thickness and number of cuticle layers of each hair measured at the same time were not significantly different and were about 3 μm and 6 to 7, indicating that the signal obtained by the above method is derived from the hair cuticle layer.
その結果、毛髪ＨＢ、ＨＰ、ＨＨではアミドIIの信号（１５４０ｃｍ−１付近）の強度が非常に強く、１５８０ｃｍ−１付近のＣＯＯ−逆対称伸縮振動の信号は判別できなかった。一方、重水処理した毛髪ＤＢ、ＤＰ、ＤＨでは、水素／重水素交換によってアミドIIの信号は１４４０ｃｍ−１付近にシフトし、１５８０ｃｍ−１付近のＣＯＯ−逆対称伸縮振動の信号が判別可能となった。表１に重水処理前後での信号強度の変化を例示する。 As a result, in the hair HB, HP, and HH, the intensity of the amide II signal (near 1540 cm −1 ) was very strong, and the signal of the COO - inverse symmetric stretching vibration near 1580 cm −1 could not be determined. On the other hand, in hair DB, DP, and DH treated with heavy water, the signal of amide II is shifted to around 1440 cm −1 by hydrogen / deuterium exchange, and the signal of COO - inverse symmetric stretching vibration around 1580 cm −1 can be discriminated. It was. Table 1 illustrates the change in signal intensity before and after heavy water treatment.
重水処理した毛髪ＤＢ、ＤＰ、ＤＨでは、１５８０ｃｍ−１付近のＣＯＯ−逆対称伸縮振動の信号強度は、各々０．１６２、０．１７５、０．１５３であった。
化学処理を行ったことがない健康な毛髪を用い上記方法にて得た信号強度をＩＨ、化学処理された毛髪を用い上記方法にて得た信号強度をＩＣとからイオン化ダメージ度（ＩＣ−ＩＨ）／ＩＨを算出して、毛髪タンパク質のカルボキシ基のイオン化ダメージ度を求めると、ブリーチ処理毛髪（ＤＢ）は０．０６、パウダーブリーチ処理毛髪（ＤＰ）は０．１１であった。結果を表２に示す。
In the heavy water-treated hair DB, DP, and DH, the signal intensity of the COO - inverse symmetric stretching vibration near 1580 cm −1 was 0.162, 0.175, and 0.153, respectively.
The signal intensity obtained by the above method using healthy hair that has not been chemically treated is I H , and the signal intensity obtained by the above method using chemically treated hair is obtained from I C based on the ionization damage degree (I By calculating C- I H ) / I H and determining the degree of ionization damage of the carboxy group of the hair protein, the bleached hair (DB) was 0.06 and the powder bleached hair (DP) was 0.11. It was. The results are shown in Table 2.
上記重水処理した毛髪ＤＢ、ＤＨについて、毛髪表面の滑り性を摩擦感テスター（カトーテック株式会社製、ＫＥＳ−ＳＥ）を用いて測定したところ、ブリーチ処理毛髪（ＤＢ）は０．１３４（平均摩擦係数）となり、健康毛髪（ＤＨ）の０．１０４に比べ有意に大きく、滑り性が低下していた。 About the hair DB and DH treated with heavy water, when the slipperiness of the hair surface was measured using a friction tester (KES-SE, manufactured by Kato Tech Co., Ltd.), the bleached hair (DB) was 0.134 (average friction The coefficient was significantly greater than 0.104 for healthy hair (DH), and slipperiness was reduced.
化学処理であるブリーチ処理によって毛髪タンパク質に変化が起こり、その結果、毛髪表面の滑り性が低下することは知られているが、その原因の一つとして、
毛髪タンパク質のカルボキシ基のイオン化の現象を捉えることができた。
It is known that the bleaching treatment, which is a chemical treatment, causes a change in hair protein, resulting in a decrease in the slipperiness of the hair surface.
We were able to capture the phenomenon of ionization of the carboxy group of hair proteins.
また、ブリーチ処理よりも毛髪に対する悪影響が強いとされるパウダーブリーチ処理において、毛髪タンパク質のカルボキシ基のイオン化ダメージ度がより大きくなることを明らかにすることができた。 In addition, it has been clarified that the degree of ionization damage of the carboxy group of the hair protein is increased in the powder bleaching treatment, which is considered to have a stronger adverse effect on the hair than the bleaching treatment.
さらに、上記毛髪を酸性緩衝液で処理すると、イオン化したカルボキシ基を元の状態に戻すことが想定されるが、以下の結果が示すように、本発明の髪タンパク質のカルボキシ基のイオン化度測定方法によって、簡便に、確認することができた。 Furthermore, when the hair is treated with an acidic buffer, it is assumed that the ionized carboxy group is restored to its original state. As shown in the following results, the method for measuring the ionization degree of the carboxy group of the hair protein of the present invention Thus, it was possible to confirm easily.
健康毛髪、ブリーチ処理毛髪、ブリーチ処理後酸性緩衝液（ｐＨ４．０リン酸緩衝液）処理毛髪各々の１５８０ｃｍ−１付近のＣＯＯ−逆対称伸縮振動の信号強度は、０．１４８、０．１６３、０．１４３であった。毛髪タンパク質のカルボキシ基のイオン化ダメージ度を求めるとブリーチ処理毛髪の健康毛髪に対する値は０．１０であった。ブリーチ処理後酸性緩衝液処理毛髪の場合、健康毛髪に対する値は−０．００となり、酸性緩衝液処理によってブリーチ処理毛髪が元の健康毛髪と同程度にまでカルボキシ基のイオン化ダメージ度が回復した。ブリーチ処理後酸性緩衝液処理毛髪のブリーチ処理毛髪に対する値は−０．１２となり、負の値を示した。カルボキシ基のイオン化ダメージ度がどの程度回復したか評価する指標となり得る。表３に結果を示す。 The signal intensity of COO - inverse symmetric stretching vibration around 1580 cm −1 for each of healthy hair, bleached hair, and bleached acid buffer (pH 4.0 phosphate buffer) treated hair is 0.148, 0.163, It was 0.143. When the degree of ionization damage of the carboxy group of the hair protein was determined, the value of bleached hair with respect to healthy hair was 0.10. In the case of acidic buffer-treated hair after bleaching treatment, the value for healthy hair was -0.00, and the degree of ionization damage of the carboxy group was restored to the same level as that of the original healthy hair by the acidic buffer treatment treatment. The value of the bleached hair after the bleach treatment with respect to the bleached hair was −0.12, indicating a negative value. It can be an index for evaluating how much the degree of ionization damage of the carboxy group has been recovered. Table 3 shows the results.
毛髪表面の滑り性もこれに対応して、ブリーチ処理毛髪、ブリーチ処理後酸性緩衝液処理毛髪各々の平均摩擦係数は、０．１３４、０．１２３となり、有意に滑り性の回復が確認できた。 Correspondingly to the slipperiness of the hair surface, the average friction coefficients of the bleached hair and the hair after acidic treatment with the bleach buffer treatment were 0.134 and 0.123, respectively, and it was confirmed that the slipperiness was significantly recovered. .
Claims (8)
前記被測定物質を赤外吸収分光法によって測定する工程と、
を含む測定方法を用いて毛髪タンパク質について測定した赤外吸収スペクトルのうち、１５８０ｃｍ−１付近のＣＯＯ−逆対称伸縮振動の信号強度を測定することを特徴とする毛髪タンパク質のカルボキシ基のイオン化度測定方法。 A step of contacting the hair with heavy water to perform hydrogen / deuterium exchange, a step of measuring the substance to be measured by infrared absorption spectroscopy, and
Measurement of the degree of ionization of the carboxy group of hair protein, wherein the signal intensity of COO - inverse symmetric stretching vibration in the vicinity of 1580 cm −1 is measured among infrared absorption spectra measured for hair protein using a measurement method comprising Method.
It hair protein of claim 1 to 3 for calculating these from the ionization damage value (I n -I 0) / I 0 when the respective signal intensities I 0, I n before and after the chemical treatment A method for measuring the degree of ionization damage of a carboxy group of a hair protein.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2005287853A (en) * | 2004-04-01 | 2005-10-20 | Pola Chem Ind Inc | Evaluation method for hair |
| JP2009210567A (en) * | 2008-02-05 | 2009-09-17 | Kao Corp | Method for measuring natural moisturizing factor by infrared absorption spectrum |
| JP2010256104A (en) * | 2009-04-23 | 2010-11-11 | Kao Corp | Evaluation method |
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| JP2005287853A (en) * | 2004-04-01 | 2005-10-20 | Pola Chem Ind Inc | Evaluation method for hair |
| JP2009210567A (en) * | 2008-02-05 | 2009-09-17 | Kao Corp | Method for measuring natural moisturizing factor by infrared absorption spectrum |
| JP2010256104A (en) * | 2009-04-23 | 2010-11-11 | Kao Corp | Evaluation method |
Non-Patent Citations (2)
| Title |
|---|
| 奈良雅之: "「赤外分光法によるタンパク質の構造機能解析」", ＦＴＩＲ ＴＡＬＫ ＬＥＴＴＥＲ, vol. 8, JPN7020000314, April 2007 (2007-04-01), ISSN: 0004209211 * |
| 宮前裕太: "「近赤外分光法を用いた毛髪表面と内部の損傷状態の非破壊評価法の開発」", 日本化粧品技術者会誌, vol. 41巻、1号, JPN6020004418, 20 March 2007 (2007-03-20), pages 22 - 30, ISSN: 0004209212 * |
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