JP2017134042A - Liquid storing vessel, liquid suction device, analysis tool, and analyser - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a liquid storing vessel that can equalize or minimize residual liquid quantities when liquid contained in a liquid storing vessel is sucked by using a dispensation element.SOLUTION: A liquid storing vessel 11, whose liquid content is sucked by a dispensation element 71 having at its tip 71a a small hole 71b through which liquid is to be sucked is equipped with a bottom wall part 11g, a side wall part 11f erecting from the bottom wall part 11 g, a liquid storing part 11e for storing liquid so formed as to be surrounded by the bottom wall part 11g and the side wall part 11f, a bottom concave 11i disposed on the liquid storing part 11e side of the bottom wall part 11g and a contact part 11k so formed in the bottom concave 11i as to permit contact by the tip 71a and to create gaps 11m and 11n to let liquid pass downward and laterally from the tip 71a without blocking the small hole 71b when the tip 71a is brought into contact.SELECTED DRAWING: Figure 5

Description

  The present invention relates to a technique for reducing the amount of liquid remaining in a container when the liquid is sucked from the container by a dispensing element such as a pipette tip.

  Conventionally, for example, Patent Document 1 describes an analyzer that performs suction of a liquid stored in a liquid storage container using a dispensing element having a flat tip. In this analyzer, when the liquid is sucked, a slight gap is generated between the tip of the dispensing element and the bottom surface of the liquid container. According to this, since the hole at the tip of the dispensing element is not blocked, the liquid can be smoothly sucked into the dispensing element. As a result, the amount of remaining liquid in the liquid container can be suppressed.

  However, in the prior art, there are points to be improved as described below.

  That is, as described above, the analyzer described in Patent Document 1 creates a gap between the tip of the dispensing element and the bottom surface of the liquid container when sucking the liquid. Usually, the dispensing element has variations in the length direction. Further, when the dispensing element is a pipette tip, the variation in the degree of fitting to the analyzer also causes the variation in the length direction. For this reason, in the analyzer described in Patent Document 1, the gap between the tip of the dispensing element and the bottom surface of the liquid storage container is not constant, and the liquid remaining in the liquid storage container is made uniform. There was a problem that I could not.

JP 2012-159358 A

  The present invention has been conceived under the circumstances as described above, and when the liquid stored in the liquid storage container is sucked using the dispensing element, the residual liquid amount is uniform or An object of the present invention is to provide a liquid container, a liquid suction device, an analysis tool, and an analysis device that can be minimized.

  The liquid storage container provided by the first aspect of the present invention is a liquid storage container in which the liquid stored therein is sucked by the dispensing element having a small hole for sucking the liquid at the tip. A bottom wall portion, a side wall portion standing up from the bottom wall portion, a liquid storage portion for storing a liquid formed so as to be surrounded by the bottom wall portion and the side wall portion, and the bottom wall portion A bottom wall recess provided on the liquid storage portion side, and the bottom wall recess is formed so that the tip can be brought into contact with each other, and the small hole is closed when the tip is brought into contact with each other. And a contact portion that creates a gap through which the liquid passes in the downward and lateral directions of the tip portion.

  Preferably, the bottom wall recess includes an upper opening and at least one inclined surface inclined downward from the upper opening, and the contact portion is formed by the at least one inclined surface. ing.

  Preferably, the upper opening has an elongated shape in a plan view, and the at least one inclined surface is formed starting from a longitudinal side of the elongated shape.

  Preferably, the bottom wall recess has a V-shaped, straight V-shaped, U-shaped, inverted trapezoidal shape, and arc-shaped shape in which the longitudinal cross-sectional shape in the short direction of the elongated shape draws a gentle curve. It is set as the structure formed in either.

  Preferably, the bottom wall recess has a longitudinal longitudinal cross-sectional shape of the elongated shape formed in any one of an arcuate shape, a semicircular shape, an inverted trapezoidal shape, a rectangular shape, a square shape, an inverted triangular shape, and an arc shape. It is supposed to be configured.

  Preferably, the elongated shape is configured to be formed into any of a spindle shape, a rectangular shape, a rounded rectangular shape, and an elliptical shape in plan view.

  Preferably, the bottom wall recess is formed in a shape of either a triangular pyramid or a quadrangular pyramid, and each of the triangular pyramid and the quadrangular pyramid has the at least one inclined surface.

  Preferably, the contact portion is configured to contact the tip portion at a contact point of 2 to 4 points.

  Preferably, the side wall portion has an inner wall surface inclined toward the bottom wall recess, and the inner wall surface is connected to the upper opening of the bottom wall recess.

  Preferably, the bottom wall recess is arranged at a position eccentric from the center of the liquid storage portion in plan view.

  The liquid suction device provided by the second aspect of the present invention is a liquid suction device for sucking the liquid stored in the liquid storage container provided by the first aspect of the present invention, wherein the dispensing is performed. An element, and a drive unit that holds the dispensing element, moves the dispensing element in a direction toward the contact portion, and contacts the tip portion with the contact portion. .

  Preferably, the driving unit further includes an elastic member that holds the dispensing element so as to be displaceable in a direction opposite to the direction toward the contact portion, and the elastic member is driven by the driving unit. When the tip portion comes into contact with the contact portion, the tip portion is elastically deformed, and the dispensing element is allowed to be displaced in the opposite direction, and the tip portion is brought into contact with the contact portion with a predetermined contact force. It is set as the structure made to contact | abut.

  Preferably, the elastic member is a compression spring.

  The liquid suction device provided by the third aspect of the present invention is a liquid suction device for sucking the liquid stored in the liquid storage container provided by the first aspect of the present invention, wherein the dispensing is performed. A second element that holds the dispensing element, moves the tip of the dispensing element in a first direction toward the abutment, and intersects the dispensing element in the first direction A drive unit that holds the displaceable element in the first direction in accordance with the movement of the tip in the first direction along the inner wall surface. It is characterized by being displaced in the direction of 2.

  The analysis tool provided by the fourth aspect of the present invention includes a reaction tank for performing an analysis reaction for analyzing a specific component in a sample by sequentially exchanging a plurality of types of liquids, and the reaction The tank is a liquid storage container provided by the first aspect of the present invention.

  The analyzer provided by the fifth aspect of the present invention is an analyzer for analyzing a specific component in a sample, and uses the analysis tool provided by the fourth aspect of the present invention. The liquid suction device provided by the second aspect, and a detection unit for detecting an analysis reaction generated in the analysis tool.

  According to one embodiment of the present invention, when the liquid contained in the liquid container is sucked using the dispensing element, the remaining amount of the liquid in the liquid container can be made uniform or minimal. .

  Other features and advantages of the present invention will become more apparent from the following description of embodiments of the present invention with reference to the accompanying drawings.

1 is a schematic configuration diagram illustrating an example of an analysis system including a liquid storage container, an analysis tool, a liquid suction device, and an analysis device according to a first embodiment of the present invention. FIG. 2A is a perspective view of an analysis tool constituting the analysis system shown in FIG. FIG. 2B is an exploded perspective view of the analysis tool shown in FIG. It is sectional drawing which shows an example of the liquid transfer operation | movement of the analyzer contained in the analysis system shown in FIG. It is a perspective view of the liquid storage container which comprises the analysis tool shown to FIG. 2 (A). FIG. 5A is a cross-sectional view taken along line VA-VA in FIG. FIG. 5B is a cross-sectional view taken along the line VB-VB in FIG. 6A to 6C are cross-sectional views illustrating an example of an operation in which the liquid suction device included in the analysis system illustrated in FIG. 1 sucks the liquid stored in the liquid storage container illustrated in FIG. . FIGS. 7A to 7D are cross-sectional views illustrating an example of an operation in which the liquid stored in the liquid storage container illustrated in FIG. 4 is sucked. It is sectional drawing which shows the liquid container which concerns on the 2nd Embodiment of this invention contained in the analysis system shown in FIG. FIG. 9A is a cross-sectional view taken along the line IXA-IXA in FIG. 8 and shows an operation of sucking the liquid stored in the liquid storage container. FIG. 9B is a cross-sectional view taken along line IXB-IXB in FIG. 8 and shows an operation of sucking the liquid stored in the liquid storage container. FIG. 10A is a cross-sectional view showing a liquid container according to the third embodiment of the present invention included in the analysis system shown in FIG. FIG. 10B is a cross-sectional view taken along line XB-XB in FIG. 10A, and shows an operation of sucking the liquid stored in the liquid storage container. FIG. 11A is a cross-sectional view showing a liquid container according to the fourth embodiment of the present invention included in the analysis system shown in FIG. FIG. 11B is a cross-sectional view taken along line XIB-XIB in FIG. 11A and shows a state in which the liquid stored in the liquid storage container is sucked. FIG. 12A is a perspective view showing an analysis tool including a liquid container according to the fifth embodiment of the present invention included in the analysis system shown in FIG. FIG. 12B is a cross-sectional view taken along line XIIB-XIIB in FIG. FIG. 13A is a cross-sectional view showing a state in which the liquid stored in the liquid storage container constituting the analysis tool shown in FIG. FIG. 13B is a cross-sectional view taken along line XIIIB-XIIIB in FIG. It is a top view which shows the liquid container which concerns on the 6th Embodiment of this invention contained in the analysis system shown in FIG. FIG. 15A is a cross-sectional view taken along line XVA-XVA in FIG. FIG. 15B is a cross-sectional view taken along line XVB-XVB in FIG.

  Hereinafter, preferred embodiments of the present invention will be specifically described with reference to the drawings. In the following description, directions such as the up and down direction are as described in the drawings. In addition, numerical ranges indicated using “to” indicate ranges including the numerical values described before and after “to” as the minimum value and the maximum value, respectively.

<First Embodiment>
The analysis system AS to which the liquid container of the present invention is applied is used for analyzing a specific component contained in a biological sample. Specifically, the analysis system AS is installed in a hospital or a veterinary hospital, and is used for detection or quantification of a specific component contained in a biological sample S such as blood or urine of a human or animal. As shown in FIG. 1, the analysis system AS includes an analysis tool 1 and an analysis device 2. The biological sample S corresponds to an example of the sample referred to in the present invention. As the biological sample S, liquids such as body fluids and solids such as feces are used. In the case of using a liquid sample, pretreatment for dilution with a diluent is performed as necessary. When analyzing a solid sample, pretreatment is performed by dissolving with a dissolving solution or suspending with a suspension as necessary. Specific examples of the biological sample S include human and animal blood, urine, saliva, serum, plasma, and feces.

[Analysis tools]
As shown in FIGS. 2A and 2B, the analysis tool 1 includes, for example, a substrate 10, a reaction tank 11, a plurality of tanks 12, and a photometric well 13. The analysis tool 1 is, for example, for examining a specific component in the biological sample S by an immunological measurement method. As an immunological measurement method, for example, an enzyme antibody method is employed. As the enzyme antibody method, for example, the ELISA method is adopted. For example, a sandwich method is employed as the ELISA method. Specific components to be analyzed include, for example, rheumatoid factor (RF), carcinoembryonic antigen (CEA), α-fetoprotein (AFP), and HIV antibody. These are contained in serum which is an example of the biological sample S.

  As shown in FIG. 2B, the substrate 10 is for mounting and integrating the reaction tank 11, the plurality of tanks 12, and the photometric well 13. As shown in FIGS. 2A and 2B, the surface of the substrate 10 is affixed with a black color or a black seal in order to prevent light from leaking from the optical system 5. The substrate 10 is molded from a synthetic resin. Specifically, for example, polystyrene (PS), polymethyl methacrylate (PMMA), polyethylene terephthalate (PET), polycarbonate (PC), polyethylene (PE), or polypropylene (PP) is used as the synthetic resin. The substrate 10 includes a mounting hole 10c for mounting the reaction tank 11, a mounting hole 10d for mounting a plurality of tanks 12, and a transmission hole 10f for transmitting light when the photometric well 13 is mounted. Yes.

  The reaction tank 11 is a container for performing an analysis reaction for analyzing a specific component in the biological sample S by sequentially exchanging a plurality of types of liquids. The reaction tank 11 corresponds to an example of a liquid container referred to in the present invention. The reaction tank 11 includes a main body 11a and a seal 11b. The main body 11a is formed of a synthetic resin. Specifically, for example, polystyrene (PS), polymethyl methacrylate (PMMA), polyethylene terephthalate (PET), polycarbonate (PC), polyethylene (PE), or polypropylene (PP) is used as the synthetic resin. The seal 11b is affixed to the upper surface of the flange portion 11c of the main body 11a. The seal 11b is made of, for example, aluminum foil, a multilayer film provided with aluminum foil, or a synthetic resin film. It is formed so as to be breakable by a pipette tip tip 71a described later. The main body 11a and the seal 11b are bonded by, for example, heat welding. As shown in FIG. 1, the reaction tank 11 has a fitting protrusion 11h on the lower surface of the flange 11c. As shown in FIG. 1 and FIG. 2 (B), the reaction vessel 11 inserts the main body 11a into the mounting hole 10c, and fits the fitting protrusion 11h into the fitting hole 10b provided in the substrate 10, thereby 10 is attached.

  An antibody against the specific component is immobilized on the inner surface of the main body 11a. A monoclonal antibody or a polyclonal antibody is used as the immobilized antibody. The immobilized antibody may be, for example, goat, mouse, horse, bovine, chicken, dog, human, pig, rabbit, From Rabbit, Rat, Syrian Hamster, or Xenopus. The antibody is immobilized on the inner surface of the main body 11a by an ordinary method. The reaction tank 11 is mounted on the substrate 10 after the antibody is immobilized. The detailed structure of the reaction tank 11 will be described later. Further, instead of immobilizing the antibody on the inner surface 11e of the main body 11a, an antibody-sensitized magnetic particle solution may be prepared as another reagent.

  Some synthetic resins selected as the material of the main body 11a are difficult to immobilize antibodies by physical adsorption. In that case, after performing VUV treatment, plasma treatment, chemical treatment, etc., a carboxyl group or an amino group is introduced into the inner surface of the main body 11a, and the antibody is immobilized by covalent bonding with these functional groups. In addition, a solid phase may be formed on a coating such as a self-assembled monolayer (SAM). The detailed structure of the reaction tank 11 will be described later.

  As shown in FIG. 1, FIG. 2 (A), and FIG. 2 (B), the plurality of tanks 12 are a biological sample dilution tank 12a, a biological sample dilution liquid tank 12b, a primary antibody liquid tank 12c, and a secondary antibody liquid tank. (Enzyme-labeled antibody solution tank) 12d, enzyme substrate solution tank 12e, reaction stop solution tank 12f, washing buffer solution tank 12g, and waste solution tank 12h. The plurality of tanks 12 are formed of a synthetic resin. As the synthetic resin, for example, polystyrene (PS), polymethyl methacrylate (PMMA), polyethylene terephthalate (PET), polycarbonate (PC), polyethylene (PE), or polypropylene (PP) is used. A seal 12i is attached to the upper surfaces of these tanks. The seal 12i is made of an aluminum foil, a multilayer film provided with the aluminum foil, or a synthetic resin film, and is formed to be breakable by a pipette tip tip 71a described later. As shown in FIG. 2B, the plurality of tanks 12 are mounted on the substrate 10 by being fitted into mounting holes 10 d provided in the substrate 10.

  The biological sample dilution tank 12a is used for dispensing a predetermined amount of the biological sample S and adjusting the mixed solution R1 diluted to an appropriate concentration. The biological sample diluent tank 12b is a tank filled with a biological sample diluent R2 for diluting the biological sample S. The biological sample S dispensed in the biological sample dilution tank 12a is diluted to a predetermined concentration by the biological sample diluent R2. As the biological sample diluent R2, for example, a phosphate buffer is used.

  The primary antibody solution tank 12c is a tank in which the primary antibody solution R3 is accommodated. The primary antibody is an antibody against the specific component, similar to the immobilized antibody, and a monoclonal antibody or a polyclonal antibody is used. The primary antibody is obtained from, for example, the above animal species in the same manner as the immobilized antibody. In the primary antibody solution R3, the primary antibody is dissolved in, for example, a phosphate buffer.

  The secondary antibody solution tank 12d is a tank in which a secondary antibody (enzyme-labeled antibody) solution R4 is accommodated. The enzyme-labeled antibody is dissolved in, for example, a phosphate buffer. The secondary antibody is an antibody against the primary antibody, and a monoclonal antibody or a polyclonal antibody is used. The secondary antibody is obtained from, for example, the above animal species in the same manner as the immobilized antibody. The secondary antibody is labeled with horseradish peroxidase (HRP). The secondary antibody is labeled with, for example, alkaline phosphatase (AP) in addition to HRP.

The enzyme substrate liquid tank 12e is a tank in which an enzyme substrate liquid R5 is accommodated as a reagent for detecting the specific component. Examples of the enzyme substrate include a fluorescent substrate, a chemiluminescent substrate, and a color substrate. When the labeling enzyme is HRP, hydrogen peroxide (H ₂ O ₂ ) is added in addition to the above. The enzyme substrate solution R5 is adjusted to a predetermined pH according to the type. H ₂ O ₂ may be prepared as a separate reagent.

  The fluorescent substrate is used for fluorescence detection of the specific component. In fluorescence detection, the presence or amount of the specific component is detected by detecting fluorescence emission emitted when excitation light is irradiated to a fluorescent substance generated when the fluorescent substrate is decomposed by the labeling enzyme. When the labeling enzyme is HRP, specific examples of the fluorescent substrate include 4-hydroxy-3-methoxyphenylacetic acid, reduced phenoxazine, reduced benzothiazine, or reduced dihydroxanthene. When the labeling enzyme is AP, specific examples of the fluorescent substrate include, for example, 4-methylumbelliferyl phosphate (4-MUP), 2- (5′-chloro-2′-phosphoryloxyphenyl) -6-chloro- 4- (3H) -quinazolinone (CPPCQ), 3,6-fluorescein diphosphate (3,6-FDP), Fast Blue-BB, Fast Red TR, or Fast Red Violet LB diazonium salt It is done.

On the other hand, the chemiluminescent substrate is used for chemiluminescent detection of the specific component. In chemiluminescence detection, the presence or amount of the specific component is detected by detecting chemiluminescence emitted by a chemiluminescent substance generated when the chemiluminescent substrate is decomposed by the labeling enzyme. When the labeling enzyme is HRP, specific examples of chemiluminescent substrates include luminol-based chemiluminescent substrates. When the labeling enzyme is AP, specific examples of the chemiluminescent substrate include, for example, 3- (2′-spiroadamantane) -4-methoxy-4- (3′-phosphoryloxy) phenyl-1,2-dioxetane Sodium salt (AMPPD), 2-chloro-5- {4-methoxyspiro [1,2-dioxetane-3,2 ′-(5′-chloro) tricyclo [3.3.1.1 ^3,7 ] decane] -4-yl} phenyl phosphate disodium salt (CDP-Star®), 3- {4-methoxyspiro [1,2-dioxetane-3,2 ′-(5′-chloro) tricyclo [3. 3.1.1 ^3,7 ] decane] -4-yl} phenyl phosphate disodium salt (CSPD®), [10-methyl-9 (10H) -acridinylidene] phenoxymethylphosphorus Acid-disodium salt (Lumigen®, APS-5), or 9- (4-chlorophenylthiophosphoryloxymethylidene) -10-methylacridan disodium salt.

  The color reaction substrate is used for color reaction detection of the specific component. In the color reaction detection, the presence or amount of the specific component is detected by detecting the color produced when the color substrate is decomposed by the labeling enzyme. When the labeling enzyme is HRP, specific examples of the colored substrate include, for example, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), o-phenylenediamine (OPD), 3, 3 ', 5,5'-tetramethylbenzidine (TMB), o-dianisidine, 5-aminosalicylic acid, 3-dimethylaminobenzoic acid (DMAB), 3-methyl-2-benzothiazoline hydrazone (MBTH), 3-amino- 9-ethylcarbazole (AEC), or 3,3′-diaminobenzidine tetrahydrochloride (DAB). When the labeling enzyme is AP, specific examples of the color substrate include p-nitrophenyl phosphate (p-NPP), 5-bromo-4-chloro-3-indolyl phosphate (BCIP), nitro blue tetrazolium ( NBT), Fast-Red, naphthol-AS, or TS phosphate.

  The reaction stop solution tank 12f is a tank for containing the reaction stop solution R6. The reaction stop solution R6 is for stopping the reaction between the secondary antibody labeling enzyme and the enzyme substrate. As the reaction stop solution R6, for example, a sulfuric acid aqueous solution or a sodium hydroxide aqueous solution is used.

  The washing buffer tank 12g is a tank for storing the washing buffer R7. The washing buffer R7 is prepared for washing the pipette tip 71 and for washing the reaction tank 11. As the washing buffer R7, for example, a phosphate buffer or a Tris buffer is used. The surfactant Tween 20 (registered trademark) is added to these buffers. A plurality of washing buffer tanks 12g may be provided according to the amount of the washing buffer R7 used.

  The waste liquid tank 12h is a tank for discarding the reagent liquid (R1 to R6) or the washing buffer R7 used in the reaction tank 11 as a waste liquid 8 described later. A plurality of waste liquid tanks 12h may be provided according to the amount of liquid to be discarded.

  Hereinafter, the mixed solution R1, the biological sample diluent R2, the primary antibody solution R3, the secondary antibody solution R4, the enzyme substrate solution R5, the reaction stop solution R6, and the washing buffer solution R7 are collectively referred to as the liquid L. .

  The photometric well 13 is a container for performing measurement by sequentially exchanging the liquid L in the reaction tank 11 and dispensing a predetermined amount of the measurement liquid L1 that has been reacted. The photometric well 13 is formed of a transparent synthetic resin. As the synthetic resin, for example, polystyrene (PS), polymethyl methacrylate (PMMA), polyethylene terephthalate (PET), polycarbonate (PC), polyethylene (PE), or polypropylene (PP) is used. As shown in FIG. 1, the photometric well 13 has a fitting protrusion 13a. The photometric well 13 is attached to the substrate 10 by fitting the fitting protrusion 13a into the fitting hole 10e provided in the substrate 10 shown in FIG. The analysis tool 1 may be one in which the substrate 10, the reaction tank 11, the plurality of tanks 12, the photometric well 13, and the microtube 30 containing the biological sample S are integrally formed in any combination. In addition, the analysis tool 1 may be integrally formed with a part on which the pipette tip 71 is placed either before or after use or before and after use.

[Analysis equipment]
As shown in FIG. 1, the analyzer 2 is for setting an analysis tool 1 at a predetermined location inside and analyzing a specific component contained in the biological sample S. The analyzer 2 includes a biological sample rack 3, a control unit 40, an input unit 42, a display unit 43, a detection unit 5, and a dispensing unit 6.

  The biological sample rack 3 is for placing the microtube 30 containing the biological sample S and the pipette tips 71 before and after use. The biological sample rack 3 is formed of a synthetic resin. For example, polystyrene (PS), polycarbonate (PC), polyethylene (PE), or polypropylene (PP) is used as the synthetic resin. As will be described later, the biological sample rack 3 is mounted on the mounting table 51 together with the analysis tool 1.

  The control unit 40 includes a Z-axis motor 8b and a pump 70 that constitute the dispensing unit 6, an optical system 50 and an X-axis motor 9b that constitute the detection unit 5, an input unit 42, and a display via a control line 41. Connected to the unit 43. The control unit 40 includes a central processing unit (CPU) (not shown) and a memory (not shown). The control unit 40 includes a read only memory (ROM) and a random access memory (RAM) as memories. The CPU reads a program stored in the ROM, performs control processing of each of the above parts, and performs analysis processing of analysis data. The CPU uses the RAM as a working memory when performing these processes.

  The input unit 42 is a part used for inputting data necessary for analysis or selecting a selection item displayed on the display unit 43 described later. Specific examples of the input unit include a keyboard and a barcode reader. Specific examples of input data include patient ID numbers, analysis items, and parameters required for analysis.

  The display unit 43 is for displaying, for example, selection items and analysis results necessary for analysis. A specific example of the display unit is a liquid crystal monitor, for example.

  The detection unit 5 includes an optical system 50, a mounting table 51, and a horizontal driving unit 9. The optical system 50 includes a light emitting element 50a and a light receiving element 50b. The light emitting element 50a is for irradiating the photometric well 13 of the analytical tool 1 with excitation light in the direction indicated by the arrow N1 when a fluorescent substrate is used as the enzyme substrate. The timing of excitation light irradiation is controlled by the control unit 40. The light receiving element 50b is for receiving, for example, fluorescence emitted from the photometric well 13 in the direction indicated by the arrow N1. The control unit 40 calculates the analysis result based on the data acquired by the light receiving element 50b.

  When a color substrate is used as the enzyme substrate, the light emitting element 50a irradiates the photometric well 13 with ultraviolet light or visible light in the direction indicated by N1. The light receiving element 50b receives the transmitted light emitted from the photometric well 13 in the direction indicated by N1.

  When a chemiluminescent substrate is used as the enzyme substrate, it is not necessary to irradiate the photometric well 13 with light by the light emitting element 50a. The light receiving element 50b receives chemiluminescence emitted from the photometric well 13, for example, in the direction indicated by N1.

  The mounting table 51 is a table for mounting the analysis tool 1 and the biological sample rack 3. The mounting table 51 holds the analysis tool 1 so that the openings of the reaction tank 11, the plurality of tanks 12, and the photometric well 13 face upward. The mounting table 51 holds the biological sample rack 3 so that the opening of the microtube 30 faces upward.

  The horizontal driving unit 9 is for moving the mounting table 51 in the X-axis direction (horizontal direction) orthogonal to the Z-axis direction. That is, the horizontal direction drive unit 9 moves the reaction tank 11, the plurality of tanks 12, the photometric well 13, and the biological sample rack 3 on the analysis tool 1 in the horizontal direction with respect to the nozzle 7 as necessary. Here, the X-axis direction indicates the horizontal direction. The horizontal driving unit 9 includes a linear stage 9a and an X-axis motor 9b. The linear stage 9 a includes a feed screw 90, a guide member 91, and a moving base 92. The moving base 92 is engaged with a feed screw 90 and a guide member 91 extending in the X-axis direction. Further, the movable table 92 is joined to the bottom surface 51 a of the mounting table 51 and holds the mounting table 51. The X-axis motor 9 b is fixed to the housing (not shown) of the analyzer 2, and moves the moving base 92 in the X-axis direction along the guide member 91 by rotating the feed screw 90. The X-axis motor 9b is connected to the control unit 40, and the operation is controlled by the control unit 40.

  As shown in FIG. 1, the dispensing unit 6 includes a nozzle 7, a pump 70, a compression spring 73, and a lifting drive unit 8. The dispensing unit 6 corresponds to an example of the liquid suction device referred to in the present invention. The dispensing unit 6 can perform not only liquid suction but also dispensing. The dispensing unit 6 sucks, moves, and dispenses the biological sample S, the liquid L, or the measurement liquid L1, and thereby the biological sample S is interposed between the reaction tank 11, the plurality of tanks 12, and the photometric well 13. The liquid L or the measurement liquid L1 is transferred. Further, the liquid L or the measurement liquid L1 is repeatedly aspirated and discharged to perform stirring.

  The nozzle 7 includes a nozzle body 72 and a pipette tip 71. The pipette tip 71 is detachably attached to the nozzle body 72. The nozzle 7 discharges or sucks the biological sample S and other liquids from a small hole 71b provided in the pipette tip tip 71a of the pipette tip 71. The pipette tip 71 corresponds to an example of a dispensing element in the present invention. Further, the pipette tip tip 71a corresponds to an example of the tip in the present invention.

  The pipette tip 71 is disposable, and for example, polypropylene is used as a material. The pipette tip tip 71a is flat and its outer peripheral shape is circular. The pipette tip tip 71a has a diameter of 1.0 mm, for example. The dispensing unit 6 pierces the seal 11b of the reaction tank 11 and the seals 12i of the plurality of tanks 12 by the pipette tip tip 71a. The pipette tip 71 has a liquid reservoir 71c that extends upward from the small hole 71b of the pipette tip tip 71a and that stores the biological sample S, the liquid L, or the measurement liquid L1 therein. . The small hole 71b has a diameter of 0.5 mm, for example. The pipette tip 71 has an attachment portion 71d for attaching to the nozzle body 72 above the liquid storage portion 71c. The pipette tip 71 has a tapered portion 71e. The taper portion 71e is formed to have an inclination angle of 1 ° to 15 ° with respect to the axial direction of the pipette tip 71. Further, the cross section of the taper portion 71e is circular.

  The nozzle 7 may be composed of only the nozzle body 72 without adopting the pipette tip 71 as a component. For example, a liquid such as the biological sample S may be sucked or discharged by the nozzle body tip 72a of the nozzle body 72, and the nozzle body tip 72a may be cleaned as necessary. In this case, the nozzle body distal end portion 72a has a tapered shape and is configured to be able to pierce the seals 11b and 12i. In this case, the nozzle body 72 corresponds to an example of a dispensing element in the present invention. Moreover, the nozzle main body front-end | tip part 72a corresponds to an example of the front-end | tip part said by this invention.

  The nozzle body 72 is made of stainless steel, for example. The nozzle body 72 is held so as to be surrounded by a nozzle support portion 84 described later. A compression spring 73 is disposed below the nozzle support portion 84. The compression spring 73 is for moving the nozzle 7 upward to absorb an impact by contracting when the pipette tip 71 hits a bottom wall recess 11i of the reaction tank 11 described later. The compression spring 73 corresponds to an example of an elastic member in the present invention. The upper end portion of the compression spring 73 is in contact with the lower end portion of the nozzle support portion 84. The nozzle main body 72 passes through the inside of the compression spring 73 and has a pipette tip mounting portion 72b for mounting the pipette tip 71 on the nozzle main body distal end portion 72a located at the lower end. An annular recess is formed in the pipette tip mounting portion 72b, and an O-ring 72c is fitted therein. When the pipette tip mounting portion 72b of the nozzle body 72 is inserted into the attachment portion 71d of the pipette tip 71, both are fitted. On the other hand, in the axial direction of the pipette tip 71, the pipette tip 71 can be removed from the nozzle body 72 by applying a force to the pipette tip 71 in a direction in which the pipette tip 71 is separated from the nozzle body 72.

  The nozzle main body 72 is formed with a first annular groove and a second annular groove (not shown) at a middle position. A first E ring 72d and a second E ring 72e are fitted into each of the annular grooves. The first E ring 72d is disposed above the second E ring 72e. The first E ring 72d and the second E ring 72e are arranged so as to sandwich the nozzle support portion 84 and the compression spring 73. The upper surface of the second E ring 72e is in contact with the lower end portion of the compression spring 73. Thereby, the compression spring 73 is hold | maintained so that it may not fall. In addition, the lower surface of the first E ring 72 d is in contact with the upper surface of the nozzle support portion 84. Thereby, the nozzle 7 is supported by the nozzle support part 84, without falling. The nozzle body 72 has a hollow tubular shape, and connects the pipette tip 71 and a pump 70 described later.

  The pump 70 is for sucking the biological sample S, the liquid L, or the measurement liquid L1 into the pipette tip 71 and discharging it from the pipette tip 71. The pump 70 is connected to the upper end portion 72 f of the nozzle main body 72 via the tube 74. The pump 70 is connected to the control unit 40, and suction and discharge operations are controlled by the control unit 40.

  The elevating drive unit 8 is for elevating and lowering the nozzle 7 in the Z-axis direction with the pipette tip tip 71a facing downward. Here, the Z-axis direction indicates the vertical direction. The elevating drive unit 8 corresponds to an example of a drive unit in the present invention. The lifting drive unit 8 holds the pipette tip 71 via the nozzle body 72. The raising / lowering drive part 8 raises / lowers the pipette tip front-end | tip part 71a to a Z-axis direction by reciprocatingly moving the nozzle 7. FIG. The lift drive unit 8 includes a linear stage 8a and a Z-axis motor 8b. The linear stage 8a includes a feed screw 80, a guide member 81 extending in the Z-axis direction, and a moving table 82. The moving table 82 includes a moving table main body 83 and a nozzle support portion 84. The movable base body 83 engages with the feed screw 80 and the guide member 81 and holds the nozzle 7 so as to be vertically movable within a predetermined range via the nozzle support portion 84. The nozzle support portion 84 is joined and integrated with the movable table main body 83 by the joining portion 85. The Z-axis motor 8b is fixed to the housing (not shown) of the analyzer 2, and the carriage 82 is reciprocated along the guide member 81 in the Z-axis direction by rotating the feed screw 80 of the linear stage 8a. Move. The Z-axis motor 8b is connected to the control unit 40, and the operation is controlled by the control unit 40.

  As shown in FIG. 1, when the dispensing unit 6 punctures the seal 11b of the reaction tank 11 and the seals 12i of the plurality of tanks 12, the tip of the pipette tip in the piercing direction (first direction) indicated by the arrow N2 The part 71a is moved. In FIG. 1, the drilling direction indicated by the arrow N2 is, for example, a direction along the Z axis. The nozzle support portion 84 has a sleeve shape, and the nozzle main body 72 passes through the inside of the nozzle support portion 84. The nozzle support portion 84 supports the nozzle body 72 so as to surround the periphery of the nozzle body 72. The nozzle support portion 84 is formed so that a gap 84 a is formed between the nozzle support portion 84 and the nozzle body 72.

  By providing the gap 84 a, the nozzle 7 has play with respect to the nozzle support portion 84. Therefore, the nozzle 7 can be displaced (freely) with a degree of freedom in a direction (second direction) intersecting the perforating direction indicated by the arrow N2. Therefore, the pipette tip tip 71a is also displaced in this direction. As shown in FIG. 1, the direction in which the nozzle 7 is displaced is the direction indicated by the arrow N3 that intersects the drilling direction. Note that the arrow N3 indicates not all directions but all directions intersecting the drilling direction indicated by the arrow N2.

  Next, with reference to FIG. 1 and FIG. 3, an example of the operation | movement which the analyzer 2 transfers the biological sample S, the liquid L, or the measurement liquid L1 between the tanks of the analysis tool 1 in the analysis system AS is demonstrated. . The transfer of the biological sample S, the liquid L, or the measurement liquid L1 is executed according to the operation control of the elevation drive unit 8 and the horizontal direction drive unit 9 by the control unit 40.

  As shown in FIG. 3, first, the biological sample rack 3 moves along the X axis and is arranged at the reference position B. The nozzle body 72 moves downward along the Z axis in the direction indicated by the arrow N2, and the pipette tip 71 is attached to the nozzle body 72. Thereafter, the pipette tip 71 is arranged at a predetermined height at the reference position B. Next, the washing buffer tank 12g moves to the reference position B. The pipette tip 71 moves downward toward the washing buffer tank 12g, and by driving the pump 70, a predetermined amount of the washing buffer R7 is sucked from the washing buffer tank 12g, and moves upward along the Z axis. Subsequently, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward and discharges the washing buffer R7 to the reaction tank 11. After elapse of a predetermined time, the pipette tip 71 sucks the washing buffer R7 from the reaction tank 11 and moves upward. Subsequently, the waste liquid tank 12 h moves to the reference position B. The pipette tip 71 moves downward toward the waste liquid tank 12h, and discharges the washing buffer R7 as the waste liquid R8.

  Next, the biological sample diluent tank 12b moves to the reference position B. The pipette tip 71 moves downward, sucks a predetermined amount of the biological sample diluent R2 from the biological sample diluent tank 12b, and moves upward. Subsequently, the biological sample dilution tank 12a moves to the reference position B. The pipette tip 71 moves downward toward the biological sample dilution tank 12a, and moves upward after discharging the biological sample diluent R2. Next, the biological sample rack 3 moves to the reference position B. The pipette tip 71 moves downward, aspirates a predetermined amount of the biological sample S from the microtube 30 and moves upward. Thereafter, the biological sample dilution tank 12a moves to the reference position B. The pipette tip 71 moves downward toward the biological sample dilution tank 12a and discharges the biological sample S. Then, the pipette tip 71 mixes the biological sample S and the biological sample diluent R2 by suction and discharge to adjust the mixed solution R1. By this operation, the biological sample S is diluted to a predetermined magnification.

  Next, the pipette tip 71 aspirates a predetermined amount of the mixed solution R1 from the biological sample dilution tank 12a. Thereafter, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11, discharges the mixed solution R1, and moves upward. As a result, the specific component in the mixed solution R1 binds to the antibody immobilized on the reaction tank 11. After incubation for a predetermined time, the pipette tip 71 moves downward, sucks the mixed solution R1 from the reaction tank 11, and moves upward. Thereafter, the waste liquid tank h moves to the reference position B. The pipette tip 71 moves downward, discards the mixed liquid R1 as the waste liquid R8 in the waste liquid tank h, and then moves upward. Next, the washing buffer tank 12g moves to the reference position B. The pipette tip 71 moves downward toward the washing buffer tank 12g, sucks a predetermined amount of the washing buffer R7, and moves upward. Subsequently, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11 and discharges the washing buffer R7. Thereafter, the pipette tip 71 quickly aspirates the washing buffer R7 and moves upward. Subsequently, the waste liquid tank 12 h moves to the reference position B. The pipette tip 71 moves downward toward the waste liquid tank 12h, and discards the washing buffer R7 as the waste liquid R8. The pipette tip 71 repeats this cleaning operation a predetermined number of times.

  Next, the primary antibody solution tank 12c moves to the reference position B. The pipette tip 71 moves downward toward the primary antibody solution tank 12c, sucks a predetermined amount of the primary antibody solution R3, and moves upward. Subsequently, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11 and discharges the primary antibody solution R3. Thereby, a primary antibody couple | bonds with the said biological component. After incubation for a predetermined time, the pipette tip 71 sucks the primary antibody solution R3 from the reaction tank 11 and moves upward. Thereafter, the waste liquid tank 12 h moves to the reference position B. The pipette tip 71 moves downward and discards the primary antibody solution R3 as the waste solution R8 in the waste solution tank 12h. Next, the washing buffer tank 12g moves to the reference position B. The pipette tip 71 moves downward toward the washing buffer tank 12g, sucks a predetermined amount of the washing buffer R7, and moves upward. Subsequently, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11 and discharges the washing buffer R7. Thereafter, the pipette tip 71 quickly aspirates the washing buffer R7 and moves upward. Subsequently, the waste liquid tank 12 h moves to the reference position B. The pipette tip 71 moves downward toward the waste liquid tank 12h, discards the washing buffer R7 as the waste liquid R8, and moves upward. The pipette tip 71 repeats this cleaning operation a predetermined number of times.

  Next, the secondary antibody solution tank 12d moves to the reference position B. The pipette tip 71 moves downward toward the secondary antibody solution tank 12d, sucks a predetermined amount of the secondary antibody solution R4, and moves upward. Subsequently, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11 and discharges the secondary antibody solution R4. Thereby, the secondary antibody binds to the primary antibody. After incubation for a predetermined time, the pipette tip 71 sucks the secondary antibody solution R4 from the reaction tank 11 and moves upward. Thereafter, the waste liquid tank 12 h moves to the reference position B. The pipette tip 71 moves downward toward the waste liquid tank 12h, discards the secondary antibody liquid R4 as the waste liquid R8, and moves upward. Next, the washing buffer tank 12g moves to the reference position B. The pipette tip 71 moves downward, sucks a predetermined amount of the washing buffer R7 from the washing buffer tank 12g, and moves up. Subsequently, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11 and discharges the washing buffer R7. Thereafter, the pipette tip 71 quickly aspirates the washing buffer R7 and moves upward. Subsequently, the waste liquid tank 12 h moves to the reference position B. The pipette tip 71 moves downward toward the waste liquid tank 12h, discards the washing buffer R7 as the waste liquid R8, and moves upward. The pipette tip 71 repeats this cleaning operation a predetermined number of times.

  Next, the enzyme substrate liquid tank 12e moves to the reference position B. The pipette tip 71 moves downward toward the enzyme substrate solution tank 12e, and ascends and moves after sucking a predetermined amount of the enzyme substrate solution R5. Subsequently, the reaction vessel 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11 and discharges the enzyme substrate solution R5. As a result, the labeling enzyme of the secondary antibody reacts with the enzyme substrate contained in the enzyme substrate solution R5. After incubation for a predetermined time, the reaction stop solution tank 12f moves to the reference position B. The pipette tip 71 moves downward toward the reaction stop solution tank 12f, sucks a predetermined amount of the reaction stop solution R6, and moves upward. Then, the reaction tank 11 moves to the reference position B. The pipette tip 71 moves downward toward the reaction tank 11 and discharges the reaction stop solution R6. Thereby, when the labeling enzyme is denatured, the enzyme reaction is stopped and the measurement liquid L1 is generated. Thereafter, the pipette tip 71 sucks the measurement liquid L1 from the reaction tank 11 and moves upward. Subsequently, the photometric well 13 moves to the reference position B. The pipette tip 71 moves downward toward the photometric well 13 and dispenses the measurement liquid L1 into the photometric well 13.

  As shown in FIG. 1, when the substrate to be used is a fluorescent substrate or a colored substrate, the reaction solution is irradiated with light of a predetermined wavelength from the light emitting element 50a of the optical system 50, and the fluorescence emitted from the reaction solution. Light emission or transmitted light is received by the light receiving element 50b. When the substrate to be used is a chemiluminescent substrate, chemiluminescence emitted from the reaction solution is received by the light receiving element 50b.

[Reaction tank structure]
As shown in FIGS. 4, 5 (A), and 5 (B), the reaction vessel 11 includes the main body 11 a and the seal 11 b as described above. The main body 11a includes a flange portion 11c, an opening portion 11d, a liquid storage portion 11e, a side wall portion 11f, and a bottom wall portion 11g. The flange portion 11c has a rectangular shape with a corner, and has an upper surface and a lower surface on the opposite side. The lower surface includes a fitting protrusion 11h. The fitting protrusion 11 h is used for mounting the reaction tank 11 on the substrate 10. The upper surface is a flat surface and is a part for adhering the seal 11b.

  The opening part 11d is provided in the substantially center part of the collar part 11c. The opening portion 11d is a portion for inserting the pipette tip tip portion 71a into the liquid storage portion 11e described later. The opening portion 11d is formed in a rounded rectangular shape having a short diameter in the width direction of the flange portion 11c. Other specific examples of the opening 11d include a rectangular shape, a square shape, or an elliptical shape. The opening 11d is covered with a seal 11b. In the opening portion 11d, the boundary portion with the flange portion 11c has a round shape or a taper shape so that the pipette tip tip portion 71a can easily enter.

  The liquid container 11e is a space provided inside the main body 11a. The side wall part 11f is provided so as to stand up around the bottom wall part 11g, and the liquid storage part 11e is formed by being surrounded by the side wall part 11f and the bottom wall part 11g. As described above, the antibody against the specific component is immobilized on the inner wall surface 11o (that is, the inner surface of the main body 11a) of the side wall portion 11f (not shown). The liquid L filled in the plurality of tanks 12 is sequentially supplied to the liquid storage unit 11e by the pipette tip 71, and a reaction necessary for analyzing the specific component is performed. As a result, the measurement liquid L1 is generated. In the liquid storage portion 11e, the inner wall surface 11o of the side wall portion 11f has a slope that is gently curved toward a bottom wall recess portion 11i described later. The inner wall surface 11o is directly connected to the bottom wall recess 11i. This is to ensure that the pipette tip tip 71a abuts against the bottom wall recess 11i even when the horizontal position of the pipette tip tip 71a is displaced from the bottom wall recess 11i.

  As shown in FIG. 4, FIG. 5 (A), and FIG. 5 (B), the bottom wall 11g includes a bottom wall recess 11i. The bottom wall recess 11i smoothly sucks the liquid L or the measurement liquid L1 when the liquid L or the measurement liquid L1 accommodated in the liquid storage part 11e is aspirated by the pipette tip 71. This is a part for minimizing the remaining amount in the liquid container 11e. The bottom wall recess 11i includes an upper opening 11j and a bottom 11q. The upper opening 11j has an elongated shape in plan view. Specific examples of the elongated shape include a spindle shape, a rectangular shape, a rounded rectangular shape, or an elliptical shape.

  In the bottom wall recess 11i, two inclined surfaces 11k are formed to face each other downward from two longitudinal sides of the upper opening 11j (that is, toward the bottom 11q). As shown in FIG. 5A, the inclined surface 11k is formed in a V shape in which the longitudinal section in the short direction draws a gentle curve. Another example of the inclined surface 11k is one in which the longitudinal section in the short direction is a linear V shape, U shape, inverted trapezoidal shape, or arc shape. The inclined surface 11k corresponds to an example of the contact portion in the present invention. Note that at least one inclined surface 11k is required as the contact portion. That is, another surface facing one inclined surface 11k may be an upright surface, for example.

  As shown in FIG. 5 (A), the bottom wall recess 11i has a smaller dimension in the lateral direction, and the pipette tip tip 71a is inclined at any position in the depth direction. It is formed so as to abut. When the pipette tip tip 71a comes into contact with the inclined surface 11k, the movement of the pipette tip 71 in the depth direction stops. Therefore, a gap 11m is generated below the pipette tip tip 71a. Therefore, as shown in FIGS. 5 (A) and 5 (B), the small hole 71b of the pipette tip end portion 71a is not blocked and is opened.

  The longitudinal sectional shape in the longitudinal direction is a bow shape as shown in FIG. Other examples of the longitudinal cross-sectional shape in the longitudinal direction include a semicircular shape, an inverted trapezoidal shape, a rectangular shape, a square shape, or a linear or curved V shape having an apex around the lowest point. The dimension of the bottom wall recess 11i in the longitudinal direction is set to be larger than the dimension of the pipette tip tip 71a at least at the depth of contact. As a result, a gap 11n is formed in the longitudinal direction of the bottom wall recess 11i and in the lateral direction of the pipette tip tip 71a.

  As described above, since the gap 11m and the gap 11n are secured in the bottom wall recess 11i, the suction and discharge of the liquid L or the measurement liquid L1 by the pipette tip 71 is performed smoothly and is not hindered.

  Next, with reference to FIG. 1 and FIGS. 6 (A) to 6 (C), in the analysis system AS, the dispensing unit 6 of the analysis apparatus 2 moves from the reaction tank 11 of the analysis tool 1 to the liquid L or the measurement liquid L1. An example of the operation at the time of sucking will be described in detail. This operation is controlled by the control unit 40. 6 (A) to 6 (C), the reaction tank 11 is shown by a longitudinal cross-sectional shape in the short direction.

  As shown in FIG. 1, the X-axis motor 9 b moves the moving base 92 along the X-axis direction by rotating a feed screw 90. Thereby, as shown in FIG. 6A, the reaction tank 11 of the analysis tool 1 moves below the nozzle 7. In this operation example, the liquid L or the measurement liquid L1 is, for example, a biological sample diluent R2 that has completed the reaction with the immobilized antibody.

  As shown in FIG. 6A, the axis C1 passing through the pipette tip tip 71a is shifted by, for example, a positional deviation Δ1 from the axis C2 passing through the bottom wall recess 11i. This positional deviation Δ1 is caused by, for example, a deviation in the mounting position of the analysis tool 1. As shown in FIG. 1 and FIG. 6A, the Z-axis motor 8b rotates the feed screw 80 to move the moving base 82 along the guide member 81 in the direction indicated by the arrow N2 (first direction). Move to. The first direction is, for example, the downward direction. Thereby, the moving stand 82 moves the pipette tip end portion 71a toward the seal 11b along the axis C1 in a state where the pipe plate tip 71a is shifted from the planned suction position by Δ1, thereby breaking the seal 11b. As a result, the pipette tip end portion 71a enters the liquid storage portion 11e from the opening portion 11d.

  Next, as shown in FIG. 6 (B), the pipette tip end portion 71a moves in the first direction along the inner wall surface 11o of the side wall portion 11f. Along with the movement of the pipette tip 71a, the pump 70 is driven, and the liquid L or the measurement liquid L1 is sucked into the pipette tip 71. In the process, the nozzle 7 is displaced in a direction (second direction) intersecting the first direction indicated by the arrow N4 toward the axis C2. The second direction is, for example, the horizontal direction. The direction indicated by the arrow N4 is one direction included in the direction indicated by the arrow N3 described above. Next, as shown in FIG. 6C, the pipette tip end portion 71a further moves in the direction indicated by the arrow N2, slides in the direction indicated by N4, and is in a state along the axis C2. This movement eliminates the positional deviation Δ1.

  As shown in FIG. 6C, the pipette tip tip 71a finally comes into contact with the inclined surface 11k of the bottom wall recess 11i. When the two inclined surfaces 11k and two points on the outer periphery of the pipette tip 71a come into contact with each other, the compression spring 73 is contracted to restrict the downward movement of the pipette tip 71. At the same time, the Z-axis motor 8b stops the movement of the pipette tip tip 71a at a predetermined position. By doing so, even if the pipette tip 71 has a variation in length, the pipette tip tip 71a properly abuts against the abutting portion 11k with a pressure generated by contraction of the compression spring 73. That is, even if the length of the pipette tip 71 is short, the frequency at which the pipette tip tip 71a stays above the bottom wall recess 11i without contacting the contact portion 11k is reduced. Even when the pipette tip 71 is too long, the frequency of collision with the bottom wall portion 11g and crushing of the tip end 71a of the pet tip or breakage of the bottom wall portion 11g is reduced. Thereafter, the pump 70 is driven to suck the liquid L or the measurement liquid L1 into the pipette tip 71.

  Another example of the cause of the positional deviation Δ1 is that caused by the warp of the pipette tip 71. Even in such a case, the pipette tip tip 71a is appropriately guided to the bottom wall recess 11i by the same operation as described above.

  Next, FIGS. 7A to 7D show an example of how the liquid L or the measurement liquid L1 moves in the reaction tank 11 when the liquid L or the measurement liquid L1 is sucked. Indicates. 7A to 7D show the longitudinal sectional shape of the reaction tank 11 in the longitudinal direction. Moreover, this example is an example in the case where there is no position shift between the pipette tip tip 71a and the bottom wall recess 11i.

  As shown in FIG. 7A, the pipette tip 71 that has broken the seal 11b and entered the liquid storage portion 11e is liquid L or measurement liquid from the small hole 71b of the pipette tip end portion 71a, as shown by the arrow. Start sucking L1. Next, as shown in FIG. 7B, the pipette tip 71 moves further downward while continuing the suction of the liquid L or the measurement liquid L1. Next, as shown in FIG. 7C, the two inclined surfaces 11k and the outer peripheral portion of the pipette tip tip 71a come into contact with each other, and the pipette tip tip 71a comes into contact with the contact portion 11k. As a result, since the gap 11m and the gap 11n are secured, the small hole 71b of the pipette tip end 71a is opened (not blocked). Therefore, the liquid L or the measurement liquid L1 does not hinder the suction and discharge of the liquid L or the measurement liquid L1 from the pipette tip tip 71a. Thereafter, as shown in FIG. 7D, the liquid L or the measurement liquid L1 is further sucked into the pipette tip 71 through the gap 11m and the gap 11n as shown by arrows. Finally, most of the liquid L or measurement liquid L1 stored in the liquid storage part 11e is aspirated. The liquid L or the measurement liquid L1 in the bottom wall recess 11i is in liquid junction with the liquid in the pipette tip and is sucked by the action of surface tension. Even if it remains, the liquid L or the measurement liquid L1 remains slightly in the bottom wall recess 11i. As shown in FIG. 7C, the two inclined surfaces 11k and the outer peripheral portion of the pipette tip tip 71a are brought into contact with each other, and the pipette tip tip 71a is brought into contact with the contact portion 11k. The liquid L or the measurement liquid L1 may be started from the beginning. Further, when the liquid L or the measurement liquid L1 in the pipette tip 71 is discharged into the empty reaction tank 11, the pipette tip tip 71a may be brought into contact with the contact portion 11k. In this way, the liquid L or the measurement liquid L1 can be filled without causing bubbles to bite the bottom of the reaction vessel 11.

  In the present embodiment, even when a general-purpose pipette tip 71 having a flat pipette tip tip 71a is used, when the pipette tip tip 71a contacts the inclined surface 11k, the pipette tip tip 71a is small. The hole 71b is opened, and the gap 11m and the gap 11n are secured. Therefore, the passage of the liquid L or the measurement liquid L1 is secured, and the suction of the liquid L or the measurement liquid L1 by the pipette tip 71 is performed without being hindered. Thereby, since the residual liquid amount in the reaction tank 11 can be made uniform or minimized, contamination of the liquid L or the measurement liquid L1 can be suppressed. As a result, the accuracy of the analysis result by the analysis system AS can be improved. In addition, for example, it is not necessary to use a pipette tip that has been subjected to special processing such that the tip is cut obliquely, so that the cost of consumables can be reduced. Conversely, when the liquid L or the measurement liquid L1 is discharged from the pipette tip 71, if the pipette tip tip 71a is brought into contact with the inclined surface 11k, the pipette tip tip 71a is not blocked, and the liquid L or measurement is smoothly performed. The liquid L1 can be discharged.

  Further, when the analysis tool 1 including the reaction tank 11 is measured by the analysis device 2, the function of the compression spring 73 causes the length variation of the pipette tip 71 and the fitting condition between the pipette tip 71 and the nozzle body 72. First, the pipette tip end portion 71a uniformly contacts the contact portion 11k with an appropriate pressure. Therefore, in the analysis system AS, the small hole 71b of the pipette tip tip 71a is not blocked even when a general-purpose pipette tip 71 having a flat pipette tip tip 71a and varying in length is used. In addition, a gap 11m and a gap 11n are secured. Therefore, the passage of the liquid L or the measurement liquid L1 is ensured, so that the suction of the liquid L or the measurement liquid L1 by the pipette tip 71 is performed without being hindered. At that time, it is not necessary to attach an expensive sensor for detecting the position of the pipette tip 71a to the analyzer 2. Thereby, the cost of consumables and analyzers can be reduced. Moreover, since the residual liquid amount in the reaction tank 11 can be made uniform or minimal, contamination of the liquid L or the measurement liquid L1 can be minimized. Thereby, the accuracy of the analysis result by the analysis system AS can be improved.

  Further, in the liquid storage portion 11e, the inner wall surface 11o of the side wall portion 11f has a slope that is gently curved toward the bottom wall recess portion 11i, and is directly connected to the bottom wall recess portion 11i. The nozzle 7 is slid in the second direction along the inclination of the bottom wall recess 11i. Therefore, even when the pipette tip tip 71a is bent or the reaction tank 11 is misaligned, the pipette tip tip 71a can be reliably guided to the bottom wall recess 11i. Thereby, when the liquid L or the measurement liquid L1 is automatically sucked from the liquid storage part 11e by the dispensing part 6, the remaining liquid amount in the reaction tank 11 can be made uniform or minimal. As a result, the accuracy of the analysis result by the analysis system AS can be improved.

<Second Embodiment>
Next, with reference to FIG. 8, FIG. 9 (A), and FIG. 9 (B), a reaction tank 11A according to a second embodiment of the present invention will be described. In the reaction tank 11A, components having the same or similar functions as those of the reaction tank 11 are denoted by the same reference numerals as those of the reaction tank 11. Detailed description of these will be omitted. Similarly to the reaction tank 11, the reaction tank 11A is applied to the analysis tool 1, the analysis apparatus 2, and the analysis system AS of the first embodiment.

  As shown in FIG. 8, FIG. 9 (A), and FIG. 9 (B), the reaction tank 11A has a circular main body 11Aa, a bottom wall portion 11Ag having a bottom wall recess 11Ai and a bottom surface portion 11Ap. It is different from the reaction tank 11 by the point. As shown in FIG. 9A, the upper opening 11Aj of the bottom wall recess 11Ai has an elongated shape, like the bottom wall recess 11Ai of the reaction vessel 11. The longitudinal cross-sectional shape of the reaction tank 11A in the longitudinal direction is a bow shape. Other examples of the longitudinal cross-sectional shape in the longitudinal direction include a semicircular shape, an inverted trapezoidal shape, a rectangular shape, a square shape, or a linear or curved V shape having an apex around the lowest point. Further, as shown in FIG. 9B, the upper opening 11Aj has a spindle shape in plan view. Here, the spindle shape is a shape in which the central portion in the longitudinal direction swells and becomes gradually narrower toward both ends. Further, as shown in FIG. 8, the inclined surface 11Ak is V-shaped in the longitudinal section in the short direction. Other examples of the vertical cross-sectional shape of the inclined surface 11Ak in the short direction include a U shape, an inverted trapezoidal shape, or an arc shape. The inclined surface 11Ak corresponds to an example of the contact portion in the present invention.

  In the bottom wall portion 11Ag of the reaction tank 11A, a bottom surface portion 11Ap is provided around the bottom wall recess portion 11Ai. The bottom surface portion 11Ap is formed horizontally.

  According to the present embodiment, the bottom surface portion 11Ap is provided, and the liquid storage portion 11e can have a large capacity by increasing the bottom surface portion 11Ap. Moreover, bottom wall recessed part 11Ai is provided in bottom wall part 11Ag of 11 A of reaction tanks. Thereby, the suction of the liquid L or the measurement liquid L1 by the pipette tip 71 is smoothly performed without being inhibited. Thereby, even if it is a case where reaction tank 11A is large capacity | capacitance, the improvement of the accuracy of the analysis result using the analysis tool 1 by analysis system AS can be aimed at similarly to 1st Embodiment. As a result, since it is not necessary to use a special pipette tip, it is possible to reduce the cost of consumables.

<Third Embodiment>
Next, with reference to FIG. 10 (A) and FIG. 10 (B), the reaction tank 11B which concerns on the 3rd Embodiment of this invention is demonstrated. The reaction tank 11B is different from the reaction tank 11A according to the second embodiment in that the bottom wall portion 11Bg of the main body 11Ba has a bottom surface portion 11Bp. Since the other components are the same as those in the second embodiment, detailed description thereof is omitted.

  As shown in FIGS. 10A and 10B, the bottom surface portion 11Bp is formed to have an inclination toward the bottom wall recess 11Ai. The inclined longitudinal cross-sectional shape is linear. Accordingly, the shape of the bottom surface portion 11Bp is a conical shape. In addition, other specific examples of the inclined longitudinal sectional shape include a stepped shape, a curved shape, or a shape obtained by deforming them. In this case, the shape of the bottom surface portion 11Bp is, for example, a bowl shape.

  According to the present embodiment, the residual liquid is easily collected in the bottom wall recess 11Ai. Therefore, the suction of the liquid L or the measurement liquid L1 from the liquid storage part 11e by the pipette tip 71 is smoothly performed without being hindered. Thereby, since the residual liquid amount in the reaction tank 11B can be made uniform or minimized, contamination of the liquid L or the measurement liquid L1 can be minimized. As a result, the accuracy of the analysis result using the analysis tool 1 by the analysis system AS can be improved. In addition, the reaction vessel 11B has the same effect as that of the second embodiment.

<Fourth Embodiment>
Next, with reference to FIG. 11 (A) and FIG. 11 (B), the reaction tank 11C which concerns on the 4th Embodiment of this invention is demonstrated. The reaction tank 11C is different from the reaction tank 11A according to the second embodiment in that the bottom wall portion 11Cg of the main body 11Ca has a bottom wall recess 11Ci. The bottom wall recess 11Ci has an inclined surface 11Ck. The inclined surface 11Ck corresponds to an example of the contact portion in the present invention. Since the other components are the same as those in the second embodiment, detailed description thereof is omitted.

  As shown in FIGS. 11A and 11B, the shape of the bottom wall recess 11Ci is a quadrangular pyramid. Therefore, the opening 11Cj is a square in plan view. The bottom wall recess 11Ci has four inclined surfaces 11Ck. When the outer shape of the pipette tip tip 71a is circular, the inclined surface 11Ck and the pipette tip tip 71a are in contact with each other at four points of contact. In this case, a gap 11n is formed between the inclined surface 11Ck and the pipette tip end portion 71a, and a gap 11m is formed below the small hole 71b of the tip end portion 71a. The liquid L or the measurement liquid L1 is sucked into the pipette tip 71 as indicated by an arrow.

  The shape of the bottom wall recess 11Cj is not limited to a quadrangular pyramid. Other examples of the shape of the bottom wall recess 11Cj include, for example, a triangular pyramid, a pentagonal pyramid, or a hexagonal pyramid. In this case, the inclined surfaces 11Ck are 3, 5, and 6, respectively. Therefore, the pipette tip tip 71a contacts at three points, five points, and six points, respectively.

  According to this embodiment, bottom wall recessed part 11Ci is provided in bottom wall part 11Cg of reaction tank 11C. Therefore, for the same reason as in the first embodiment, the suction of the liquid L or the measurement liquid L1 by the pipette tip 71 is smoothly performed without being hindered. Thereby, the improvement of the accuracy of the analysis result using the analysis tool 1 by the analysis system AS can be aimed at. Further, for example, it is not necessary to use a pipette tip that has been subjected to special processing such as cutting the tip end portion 71a of the pipette tip obliquely, so that the cost of consumables can be reduced. In addition, the reaction tank 11C has the same effect as that of the second embodiment.

<Fifth Embodiment>
Next, with reference to FIG. 12 (A), FIG. 12 (B), FIG. 13 (A) and FIG. 13 (B), an analysis tool 1A according to a fifth embodiment of the present invention will be described. The analysis tool 1A is different from the analysis tool 1 to which the reaction tank 11A according to the second embodiment is applied in that it includes a reaction tank 11D, a fine flow path 15, a liquid storage portion 15b, and a seal 16. . Further, unlike the analysis tool 1, the analysis tool 1A is not set with reagents necessary for the measurement reaction. These reagents are set, for example, in a rack (not shown) provided at a predetermined location of the analyzer 2. Instead of the reaction tank 11 and the photometric well 13, the analysis tool 1 </ b> A is set in the detection unit 5. Since the other components are the same as those in the second embodiment, detailed description thereof is omitted. As another example, the analysis tool 1 </ b> A can further include a plurality of tanks 12 of the analysis tool 1. In this case, as shown in FIG. 12B, measurement is performed using a later-described measurement cell 15a provided in the middle of the fine channel 15. As yet another example, the analysis tool 1 </ b> A may be configured to include a plurality of tanks 12 and photometric wells 13 as with the analysis tool 1. In this case, measurement is performed using the photometric well 13.

  As shown in FIG. 12B, in the analysis tool 1A, the liquid storage portion 11De of the reaction tank 11D is connected to the fine channel 15 by the inner wall surface 11Do of the side wall portion 11Df. A measurement cell 15 a is provided at an intermediate portion of the fine channel 15. The measurement cell 15a is measured by the detection unit 5 of the analyzer 2. As shown in FIGS. 12A and 12B, the analysis tool 1A includes an upper member 14a, a lower member 14c, and an adhesive layer 14b. The upper member 14a and the lower member 14c are resin molded products, and are manufactured by, for example, injection molding. Since the analysis tool 1A is used for optical measurement, the upper member 14a and the lower member 14c are manufactured using a light-transmitting material. For example, polymethyl methacrylate (PMMA), polystyrene (PS), polyethylene (PE), or polypropylene (PP) is used as the material. The analysis tool 1A is assembled by bonding the upper member 14a and the lower member 14c using the adhesive layer 14b. Specifically, a double-sided tape is used as the adhesive layer 14b. The adhesive layer 14b is punched into the shape of the bottom wall recess 11Di and the fine channel 15. By the upper layer 14a and the lower member 14c being integrated by the adhesive layer 14b, the reaction tank 11D and the fine channel 15 are formed inside. A part of the side wall portion 11Df of the reaction tank 11D protrudes from the upper surface 14d of the upper member 14a.

  As shown in FIGS. 12A and 12B, an opening 14e is formed in the upper surface 14d of the upper member 14a. In order to cover the opening 14e, a seal 16 is attached around the opening 14e on the upper surface 14d. The seal 16 is made of the same material as the seal 11b described above. An air vent hole 16 a is formed in the seal 16. The air vent hole 16a is formed to smoothly perform the operation of dispensing or inhaling the liquid L or the measurement liquid L1 from the reaction tank 11D using the pipette tip 71.

  As shown in FIGS. 13A and 13B, the reaction vessel 11D includes a bottom wall portion 11Dg and a bottom wall recess 11Di. A part of the fine flow path 15 is looking into the bottom wall portion 11Dg. The bottom surface portion 11Dp of the bottom wall portion 11Dg is formed by the adhesive layer 14b. The bottom wall recess 11Di has the same structure as the bottom wall recess 11Ai of the reaction tank 11A of the second embodiment. The bottom wall recess 11Di has an inclined surface 11Dk having the same shape as the inclined surface 11Ak. The inclined surface 11Dk is formed by punching the adhesive layer 14b. The inclined surface 11Dk corresponds to an example of the contact portion in the present invention. The bottom portion 11Dq of the bottom wall recess 11Di is formed by the upper surface of the lower member 14c. Therefore, the bottom wall recess 11Di is a region surrounded by the adhesive layer 14b and the upper surface of the lower member 14c. Therefore, the pipette tip 71 aspirates the liquid L or the measurement liquid L1 as indicated by an arrow, like the reaction tank 11A. As described above, in the reaction vessel 11D, the fine channel 15 is connected to the liquid storage portion 11De on the inner wall surface 11Do. The pipette tip 71 is configured to control the movement of the liquid L or the measurement liquid L1 in the fine channel 15 by suction and discharge. The pipette tip 71 fills the fine channel 15 with the liquid L or the measurement liquid L1 when the liquid L or the measurement liquid L1 is discharged to the liquid storage portion 11De. Further, in the analysis tool 1A, the liquid L or the measurement liquid L1 is sucked and discharged by the pipette tip 71, whereby the liquid L or the measurement liquid L1 is reciprocated and stirred.

  According to the present embodiment, since the reaction tank 11D and the measurement cell 15a are integrated with each other, the liquid L is sequentially replaced and the finally generated measurement liquid L1 is transferred to a separately provided photometric well. There is no need to do. Thereby, the analysis process can be simplified. As a result, it is possible to speed up the analysis of the specific component in the biological sample S. Moreover, reagent reaction and washing can be performed efficiently by reciprocating liquid stirring. Thereby, the improvement of the accuracy of the analysis result using the analysis tool 1 by the analysis system AS can be aimed at. In addition, the analysis tool 1A can have the same effects as those of the second embodiment.

<Sixth Embodiment>
Next, with reference to FIG. 14, FIG. 15 (A), and FIG. 15 (B), the reaction tank 11E which concerns on the 6th Embodiment of this invention is demonstrated. In the reaction vessel 11E, components having the same or similar functions as the components of the reaction vessel 11 according to the first embodiment are denoted by the same reference numerals as those in the reaction vessel 11. Detailed description of these will be omitted. Similarly to the reaction tank 11, the reaction tank 11E is applied to the analysis tool 1, the analysis device 2, and the analysis system AS of the first embodiment.

  As shown in FIG. 14, FIG. 15 (A), and FIG. 15 (B), the bottom wall recess 11Ei is arranged in a position eccentric from the center of the liquid storage portion 11Ee in plan view. Is different. Specifically, the reaction tank 11E has a bottom wall recess 11Ei having an inclined surface 11Ek with which the pipette tip tip 71a abuts at a position where the bottom wall 11Eg of the main body 11Ea is eccentric from the central axis C3 of the main body 11Ea. ing. The inclined surface 11Ek corresponds to an example of the contact portion in the present invention. Here, as shown in FIG. 14, the opening 11 </ b> Ed of the liquid storage portion 11 </ b> Ee is provided at a substantially central portion of the flange portion 11 c. The opening 11Ed is formed in a rounded rectangular shape having a short diameter in the width direction of the flange portion 11c. The central axis C3 is an axis that passes through the center of the opening 11Ed. In addition to the rounded rectangular shape, the shape of the opening 11Ed includes, for example, a square shape, a rectangular shape, or an elliptical shape.

  As shown in FIG. 15B, the bottom wall recess 11Ei is provided, for example, at a position eccentric to the left from the central axis C3 of the main body 11Ea in the front view. Accordingly, the abutting portion 11Ek that abuts when the pipette tip tip 71a sucks the liquid L or the measuring liquid L1 is also eccentric to the left. Accordingly, the side wall portion 11Ef is also deformed leftward in a front view. Thereby, the inner wall surface 11Eo of the portion corresponding to the right side surface of the side wall portion 11Ef is greatly curved. Therefore, when the pipette tip tip 71a comes into contact with the contact portion 11Ek, the right volume of the pipette tip 71 becomes larger than the left side in the liquid storage portion 11Ee. Further, when the pipette tip tip 71a comes into contact with the contact portion 11Ek, the gap 11En generated in the right direction of the pipette tip tip 71a is larger than the gap 11n generated in the left direction.

  According to the present embodiment, as shown in FIG. 15 (B), the liquid L or the measurement liquid is obtained by pumping and discharging in a state where the pipette tip tip 71a is in contact with the contact portion 11Ek of the bottom wall recess 11Ei. When the L1 is stirred, the volume on the right side of the pipette tip 71 is relatively larger than that on the left side, so that when the liquid L or the measurement liquid L1 is discharged from the pipette tip 71 to the reaction vessel 11E, the pipette tip 71 When a part of the left side liquid L or the measurement liquid L1 moves to the right side of the pipette tip 71 to reach the horizontal, and conversely, when the liquid L or the measurement liquid L1 is sucked into the pipette tip 71 from the reaction tank 11E, The liquid L or the measurement liquid L1 disappears from the left side of the pipette tip 71 first. By repeating such a process, the left and right liquids L or the measurement liquid L1 of the pipette tip 71 are circulated, and the stirring efficiency of the liquid L or the measurement liquid L in the reaction tank 11E is improved. By improving the stirring efficiency, the reactivity of the antigen-antibody reaction and the efficiency of BF separation are improved. As a result, the accuracy of the analysis result by the analysis system AS can be improved.

  The present invention is not limited to the contents of the above-described embodiment. The specific configurations of the liquid container, the liquid suction device, the analysis tool, and the analysis device according to the present invention can be varied in design in various ways.

  In the first to fourth and sixth embodiments, the bottom wall recesses 11i, 11Ai, and 11Ci are exemplified by using the analysis tool 1 specially designed for analyzing a specific component contained in the biological sample S as an example. 11Ei has been described. However, the present invention is not limited to these. The bottom wall recesses 11i, 11Ai, 11Ci, and 11Ei of the present invention can also be applied to general-purpose microplates and microtubes. According to such a configuration, even with a general-purpose microplate or microtube, it is possible to efficiently suck the liquid L or the measurement liquid L1 with a small amount of remaining liquid without requiring skill.

  In the first to sixth embodiments, the bottom wall recesses 11i, 11Ai, 11Ci, 11Di, and 11Ei have been described as examples as means for preventing the small holes 71b of the pipette tip tip 71a from being blocked. However, in the present invention, the means for preventing the small hole 71b from being blocked is not limited to the concave shape. The means for preventing the small hole 71b from being blocked may have a convex shape. Specifically, a protrusion is formed on the bottom wall portion of the liquid storage container so that the small hole 71b of the pipette tip tip 71a is not blocked while the protrusion and the pipette tip tip 71a are in contact with each other. . According to such a configuration, even when a general-purpose pipette tip 71 having a flat pipette tip tip 71a is used, the suction of the efficient liquid L or the measuring liquid L1 with a small amount of residual liquid that is not necessary for skill is required. It can be performed.

L Liquid S Biological sample (sample)
1,1A Analysis tool 11, 11A-11E Liquid container 11e, 11De, 11Ee Liquid container 11f, 11Df, 11Ef Side wall 11g, 11Ag, 11Bg, 11Cg, 11Dg, 11Eg Bottom wall 11i, 11Ai, 11Ci, 11Di, 11Ei Bottom wall recess 11j, 11Aj, 11Cj Upper opening 11k, 11Ak, 11Ck, 11Dk, 11Ek Inclined surface (contact portion)
11m, 11n, 11En Gap 11o, 11Do, 11Eo Inner wall surface 2 Analyzer 5 Detector 6 Dispensing unit (liquid suction device)
7 Nozzle 71 Pipette tip (dispensing element)
71a Pipette tip tip (tip)
71b Small hole 72 Nozzle body (dispensing element)
73 Compression spring (elastic member)
72a Nozzle body tip (tip)
8 Lifting drive unit (drive unit)
84 Nozzle support (displacement means)

Claims (16)

A liquid storage container in which the liquid stored inside is sucked by a dispensing element having a small hole for sucking the liquid at the tip,
The bottom wall,
A side wall portion standing from the bottom wall portion;
A liquid storage portion for storing a liquid formed so as to be surrounded by the bottom wall portion and the side wall portion;
A bottom wall recess provided on the bottom side of the liquid container;
The bottom wall recess is formed so that the tip can be brought into contact with the liquid, and when the tip is brought into contact, the small hole is not blocked and the liquid flows downward and laterally in the tip. An abutment that creates a gap through;
A liquid container. The bottom wall recess includes an upper opening and at least one inclined surface inclined downward from the upper opening;
The liquid container according to claim 1, wherein the contact portion is formed by the at least one inclined surface. The upper opening has an elongated shape in plan view,
The liquid container according to claim 2, wherein the at least one inclined surface is formed starting from a longitudinal side of the elongated shape.   The bottom wall recess has any one of a V-shape, a straight V-shape, a U-shape, an inverted trapezoid shape, and an arc shape in which the longitudinal cross-sectional shape in the short direction of the elongated shape draws a gentle curve. The liquid container according to claim 3, wherein the liquid container is formed.   In the bottom wall recess, the longitudinal longitudinal cross-sectional shape of the elongated shape is formed in any one of a bow shape, a semicircular shape, an inverted trapezoidal shape, a rectangular shape, a square shape, an inverted triangular shape, and an arc shape. The liquid container according to claim 3 or 4.   The liquid container according to claim 3, wherein the elongated shape is formed in any of a spindle shape, a rectangular shape, a rounded rectangular shape, and an elliptical shape in plan view. The bottom wall recess is formed in any one of a triangular pyramid and a quadrangular pyramid,
The liquid container according to claim 2, wherein each of the three-sided pyramid and the four-sided pyramid has the at least one inclined surface.   The liquid container according to claim 1, wherein the contact portion is configured to contact the tip portion at a contact point of 2 to 4 points. The side wall portion has an inner wall surface that is inclined toward the bottom wall recess,
The liquid container according to claim 1, wherein the inner wall surface is connected to the upper opening of the bottom wall recess.   The liquid container according to claim 1, wherein the bottom wall recess is disposed at a position eccentric from a center of the liquid container in a plan view. A liquid suction device for sucking the liquid stored in the liquid storage container according to any one of claims 1 to 10,
The dispensing element;
A drive unit that holds the dispensing element, moves the dispensing element in a direction toward the contact portion, and contacts the tip portion with the contact portion;
A liquid suction device comprising: The drive unit holds the dispensing element so as to be displaceable in a direction opposite to the direction toward the contact unit,
An elastic member,
The elastic member is elastically deformed when the tip portion comes into contact with the contact portion by driving the drive portion, and allows the dispensing element to be displaced in the opposite direction, and has a predetermined amount. The liquid suction device according to claim 11, wherein the tip portion is brought into contact with the contact portion with contact force.   The liquid suction device according to claim 12, wherein the elastic member is a compression spring. A liquid suction device for sucking the liquid stored in the liquid storage container according to claim 9,
The dispensing element;
Holding the dispensing element, moving the tip of the dispensing element in a first direction towards the abutment, and moving the dispensing element in a second direction intersecting the first direction; A drive unit that is held displaceably;
With
The said drive part is a liquid suction device which displaces the said dispensing element to a said 2nd direction according to the said front-end | tip part moving to a said 1st direction along the said inner wall surface. Equipped with a reaction tank to perform analysis reaction to analyze specific components in the sample by sequentially exchanging multiple types of liquid,
The said reaction tank is an analysis tool which is a liquid storage container in any one of Claims 1-9. An analysis device for analyzing a specific component in a sample,
Using the analytical tool according to claim 15,
A liquid suction device according to any one of claims 11 to 14,
A detection unit for detecting an analysis reaction generated in the analysis tool;
An analysis device comprising:

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