JP2017104130A5 - - Google Patents
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- Publication number
- JP2017104130A5 JP2017104130A5 JP2017041609A JP2017041609A JP2017104130A5 JP 2017104130 A5 JP2017104130 A5 JP 2017104130A5 JP 2017041609 A JP2017041609 A JP 2017041609A JP 2017041609 A JP2017041609 A JP 2017041609A JP 2017104130 A5 JP2017104130 A5 JP 2017104130A5
- Authority
- JP
- Japan
- Prior art keywords
- flavin
- glucose dehydrogenase
- furthermore
- following
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims 6
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- 229960001031 Glucose Drugs 0.000 claims 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N β-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 241001541164 Mucor guilliermondii Species 0.000 claims 1
- 230000024881 catalytic activity Effects 0.000 claims 1
- 230000001419 dependent Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 238000001962 electrophoresis Methods 0.000 claims 1
- 229920002401 polyacrylamide Polymers 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Claims (6)
（１）分子量： ＳＤＳ−ポリアクリルアミド電気泳動で測定した該酵素のポリペプチド部分の分子量が約８８ｋＤａである。(1) Molecular weight: The molecular weight of the polypeptide portion of the enzyme measured by SDS-polyacrylamide electrophoresis is about 88 kDa.
（２）基質特異性： Ｄ−グルコースに対する反応性を１００％としたときのＤ−キシロースに対する反応性が１．３％以下である。(2) Substrate specificity: The reactivity to D-xylose is 1.3% or less when the reactivity to D-glucose is 100%.
（３）Ｋｍ値： Ｄ−グルコースに対するＫｍ値が１５ｍＭ以下。(3) Km value: Km value for D-glucose is 15 mM or less.
（４）至適活性ｐＨ：ｐＨ６(4) Optimum active pH: pH 6
（５）至適活性温度：４５℃(5) Optimal activity temperature: 45 ° C
（６）ｐＨ安定性： ｐＨ４．５〜８．０の範囲で安定(6) pH stability: stable in the range of pH 4.5 to 8.0
（７）温度安定性： ４５℃の温度で１５分間維持した後の残存酵素活性率が９０％以上。(7) Temperature stability: The residual enzyme activity rate after maintaining at a temperature of 45 ° C. for 15 minutes is 90% or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017041609A JP6288332B2 (en) | 2017-03-06 | 2017-03-06 | Novel glucose dehydrogenase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017041609A JP6288332B2 (en) | 2017-03-06 | 2017-03-06 | Novel glucose dehydrogenase |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011222278A Division JP2013081399A (en) | 2011-10-06 | 2011-10-06 | New glucose dehydrogenase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017104130A JP2017104130A (en) | 2017-06-15 |
| JP2017104130A5 true JP2017104130A5 (en) | 2017-07-27 |
| JP6288332B2 JP6288332B2 (en) | 2018-03-07 |
Family
ID=59057971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017041609A Active JP6288332B2 (en) | 2017-03-06 | 2017-03-06 | Novel glucose dehydrogenase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6288332B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX360352B (en) * | 2012-02-15 | 2018-10-30 | Hoffmann La Roche | Fc-receptor based affinity chromatography. |
| BR112017006178A2 (en) | 2014-11-06 | 2018-05-02 | F. Hoffmann-La Roche Ag | fc region, antibodies, pharmaceutical formulation and uses of antibodies |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2010053161A1 (en) * | 2008-11-06 | 2012-04-05 | ユニチカ株式会社 | Modified flavin adenine dinucleotide-dependent glucose dehydrogenase |
| WO2010140431A1 (en) * | 2009-06-04 | 2010-12-09 | キッコーマン株式会社 | Flavin-bound glucose dehydrogenase |
| WO2011034108A1 (en) * | 2009-09-16 | 2011-03-24 | 東洋紡績株式会社 | Modified flavine-adenine-dinucleotide-dependent glucose dehydrogenase |
| US8883434B2 (en) * | 2009-12-05 | 2014-11-11 | Amano Enzyme Inc. | Mutant enzyme and application thereof |
-
2017
- 2017-03-06 JP JP2017041609A patent/JP6288332B2/en active Active
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